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1
Özışık, Damla, und Nihat Akın. „Akkermansia muciniphila and Faecalibacterium prausnitzii as next generation probiotic: Implications for health“. Food and Health 11, Nr. 1 (2025): 77–90. https://doi.org/10.3153/fh25007.
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In the last decade, research in the field of probiotic microbiology has advanced significantly. Studies on the selection, characterization and validation of health claims of probiotic cultures have made significant progress, and molecular-genetic research has helped us understand the mechanistic basis of the beneficial effects of probiotics. These developments have been an important step forward in understanding the positive effects of probiotics on human health. Probiotic foods are usually found in fermented dairy products and contain microorganisms that are beneficial for health. However, while their use in other food types has increased, the use of probiotics is not yet widespread in composite foods. More research is needed to better understand and optimize the health benefits of probiotic composite foods, with an emphasis on issues such as stability, efficacy and digestibility of the probiotic ingredient. In this review, we specifically focus on two emerging new generations of probiotic bacteria, Faecalibacterium prausnitzii and Akkermansia muciniphila, as their presence in the digestive tract may have an impact on cancer incidence. The properties of these new generation probiotics, such as enhancing gastrointestinal immunity, maintaining intestinal barrier integrity, producing beneficial metabolites and enhancing immunotherapy efficacy, were examined. In addition, safety and efficacy studies on the use of new generation probiotics in cancer patients were evaluated.
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Zhai, Qixiao, Saisai Feng, Narbad Arjan und Wei Chen. „A next generation probiotic, Akkermansia muciniphila“. Critical Reviews in Food Science and Nutrition 59, Nr. 19 (29.10.2018): 3227–36. http://dx.doi.org/10.1080/10408398.2018.1517725.
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Li, Jianhong, Jing Xu, Xue Guo, Haoming Xu, Chen Huang, Yuqiang Nie und Youlian Zhou. „Odoribacter splanchnicus—A Next-Generation Probiotic Candidate“. Microorganisms 13, Nr. 4 (03.04.2025): 815. https://doi.org/10.3390/microorganisms13040815.
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As an important intestinal microorganism, Odoribacter splanchnicus frequently appears in high-throughput sequencing analyses, although pure culture research on this microorganism is not as advanced. It is widely present in the mammalian gut and is closely associated with the health status of the host and the incidence of various diseases. In recent years, changes in the abundance of O. splanchnicus have been found to be positively or negatively correlated with health issues, such as obesity, metabolic syndrome, diabetes, and intestinal inflammation. It may exhibit a dual protective or promotional role in specific diseases. Thus, it may play an important role in regulating host metabolism, immune response, and intestinal homeostasis. Additional research has revealed that O. splanchnicus can synthesize various metabolites, especially short-chain fatty acids (SCFAs), which play a key role in promoting intestinal health, enhancing energy metabolism, improving insulin resistance, and regulating immune responses in the host. Therefore, O. splanchnicus is a strong candidate for “next-generation probiotics”, and its potential probiotic function provides novel ideas for the development of functional foods and the prevention and treatment of metabolic and intestinal inflammatory diseases. These findings can help develop new biological treatment strategies and optimize health management plans.
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Sionek, Barbara, Aleksandra Szydłowska, Dorota Zielińska, Katarzyna Neffe-Skocińska und Danuta Kołożyn-Krajewska. „Beneficial Bacteria Isolated from Food in Relation to the Next Generation of Probiotics“. Microorganisms 11, Nr. 7 (30.06.2023): 1714. http://dx.doi.org/10.3390/microorganisms11071714.
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Recently, probiotics are increasingly being used for human health. So far, only lactic acid bacteria isolated from the human gastrointestinal tract were recommended for human use as probiotics. However, more authors suggest that probiotics can be also isolated from unconventional sources, such as fermented food products of animal and plant origin. Traditional fermented products are a rich source of microorganisms, some of which may have probiotic properties. A novel category of recently isolated microorganisms with great potential of health benefits are next-generation probiotics (NGPs). In this review, general information of some “beneficial microbes”, including NGPs and acetic acid bacteria, were presented as well as essential mechanisms and microbe host interactions. Many reports showed that NGP selected strains and probiotics from unconventional sources exhibit positive properties when it comes to human health (i.e., they have a positive effect on metabolic, human gastrointestinal, neurological, cardiovascular, and immune system diseases). Here we also briefly present the current regulatory framework and requirements that should be followed to introduce new microorganisms for human use. The term “probiotic” as used herein is not limited to conventional probiotics. Innovation will undoubtedly result in the isolation of potential probiotics from new sources with fascinating new health advantages and hitherto unforeseen functionalities.
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Lin, Li-Te, Chia-Jung Li, Chia-Chun Wu, Li-Fei Pan und Kuan-Hao Tsui. „Pilot Study on Next-Generation Sequencing Analysis of Vaginal Microbiota in Clinically Infertile Patients Treated with Probiotics“. Journal of Clinical Medicine 13, Nr. 12 (11.06.2024): 3420. http://dx.doi.org/10.3390/jcm13123420.
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Background: In this investigation, we aimed to understand the influence of oral probiotic supplementation on the vaginal microbiota of women preparing for assisted reproductive technology (ART) procedures. Given the importance of a healthy microbiome for reproductive success, this study sought to explore how probiotics might alter the bacterial composition in the vaginal environment. Methods: We recruited a cohort of 30 women, averaging 37 years of age (ranging from 31 to 43 years), who were scheduled to undergo ART. Using 16S ribosomal RNA (rRNA) sequencing, we meticulously analyzed the vaginal microbiota composition before and after the administration of oral probiotic supplements. Results: Our analysis identified 17 distinct microorganisms, including 8 species of Lactobacillus. Following probiotic supplementation, we observed subtle yet notable changes in the vaginal microbiota of some participants. Specifically, there was a decrease in Gardnerella abundance by approximately 20%, and increases in Lactobacillus and Bifidobacterium by 10% and 15%, respectively. Additionally, we noted a significant reduction in the Firmicutes/Bacteroidetes (F/B) ratio in the probiotic group, indicating potential shifts in the overall bacterial composition. Conclusions: These preliminary findings suggest that oral probiotic supplementation can induce significant changes in the vaginal microbiota of middle-aged women undergoing ART, potentially improving their overall bacterial profile. Future studies should consider a larger sample size and a narrower age range to validate these results. Investigating factors related to female hormone production could also provide deeper insights. Understanding the effects of probiotics on the vaginal microbiota in patients with ovarian aging may lead to personalized interventions and better reproductive outcomes.
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Štofilová, Jana, Monika Kvaková, Anna Kamlárová, Emília Hijová, Izabela Bertková und Zuzana Guľašová. „Probiotic-Based Intervention in the Treatment of Ulcerative Colitis: Conventional and New Approaches“. Biomedicines 10, Nr. 9 (09.09.2022): 2236. http://dx.doi.org/10.3390/biomedicines10092236.
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Although there are number of available therapies for ulcerative colitis (UC), many patients are unresponsive to these treatments or experience secondary failure during treatment. Thus, the development of new therapies or alternative strategies with minimal side effects is inevitable. Strategies targeting dysbiosis of gut microbiota have been tested in the management of UC due to the unquestionable role of gut microbiota in the etiology of UC. Advanced molecular analyses of gut microbiomes revealed evident dysbiosis in UC patients, characterized by a reduced biodiversity of commensal microbiota. Administration of conventional probiotic strains is a commonly applied approach in the management of the disease to modify the gut microbiome, improve intestinal barrier integrity and function, and maintain a balanced immune response. However, conventional probiotics do not always provide the expected health benefits to a patient. Their benefits vary significantly, depending on the type and stage of the disease and the strain and dose of the probiotics administered. Their mechanism of action is also strain-dependent. Recently, new candidates for potential next-generation probiotics have been discovered. This could bring to light new approaches in the restoration of microbiome homeostasis and in UC treatment in a targeted manner. The aim of this paper is to provide an updated review on the current options of probiotic-based therapies, highlight the effective conventional probiotic strains, and outline the future possibilities of next-generation probiotic and postbiotic supplementation and fecal microbiota transplantation in the management of UC.
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Jung, Hye Young, und Kwang Wook Kim. „An Evidence-Based Review of Probiotics and Prebiotics“. Science Insights 40, Nr. 6 (30.05.2022): 527–31. http://dx.doi.org/10.15354/si.22.re055.
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Probiotics and prebiotics have a variety of beneficial effects on the host’s health. Extensive studies have established probiotic strains such as Lactobacillus and Bifidobacterium, and further the concept of next-generation probiotics has been advocated. Clinical trials and mechanism of action research have demonstrated that the gut microbiota and host health are inextricably linked, and that probiotics can benefit intestinal-related disorders such as inflammatory bowel disease by controlling the gut microbiota. Accordingly, the host’s gut microbiota has the greatest direct effect on the efficiency of probiotics and prebiotics. Due to the highly individualized gut microbiota, supplementation with probiotics and prebiotics must take the host’s gut microbiota into account. Personalized and specific interventions, as well as the development of next-generation probiotics, will be the new focus of research.
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Asar, Remziye, Sinem Erenler, Dilara Devecioglu, Humeyra Ispirli, Funda Karbancioglu-Guler, Hale Inci Ozturk und Enes Dertli. „Understanding the Functionality of Probiotics on the Edge of Artificial Intelligence (AI) Era“. Fermentation 11, Nr. 5 (05.05.2025): 259. https://doi.org/10.3390/fermentation11050259.
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This review focuses on the potential utilization of artificial intelligence (AI) tools to deepen our understanding of probiotics, their mode of action, and technological characteristics such as survival. To that end, this review provides an overview of the current knowledge on probiotics as well as next-generation probiotics. AI-aided omics technologies, including genomics, transcriptomics, and proteomics, offer new insights into the genetic and functional properties of probiotics. Furthermore, AI can be used to elucidate key probiotic activities such as microbiota modulation, metabolite production, and immune system interactions to enable an improved understanding of their health impacts. Additionally, AI technologies facilitate precision in identifying probiotic health impacts, including their role in gut health, anticancer activity, and antiaging effects. Beyond health applications, AI can expand the technological use of probiotics, optimizing storage survival and broadening biotechnological approaches. In this context, this review addresses how AI-driven approaches can be facilitated by strengthening the evaluation of probiotic characteristics, explaining their mechanisms of action, and enhancing their technological applications. Moreover, the potential of AI to enhance the precision of probiotic health impact assessments and optimize industrial applications is highlighted, concluding with future perspectives on the transformative role of AI in probiotic research.
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Stastna, Miroslava. „The Role of Proteomics in Identification of Key Proteins of Bacterial Cells with Focus on Probiotic Bacteria“. International Journal of Molecular Sciences 25, Nr. 16 (06.08.2024): 8564. http://dx.doi.org/10.3390/ijms25168564.
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Probiotics can affect human health, keep the balance between beneficial and pathogenic bacteria, and their colonizing abilities enable the enhancement of the epithelial barrier, preventing the invasion of pathogens. Health benefits of probiotics were related to allergy, depression, eczema, cancer, obesity, inflammatory diseases, viral infections, and immune regulation. Probiotic bacterial cells contain various proteins that function as effector molecules, and explaining their roles in probiotic actions is a key to developing efficient and targeted treatments for various disorders. Systematic proteomic studies of probiotic proteins (probioproteomics) can provide information about the type of proteins involved, their expression levels, and the pathological changes. Advanced proteomic methods with mass spectrometry instrumentation and bioinformatics can point out potential candidates of next-generation probiotics that are regulated under pharmaceutical frameworks. In addition, the application of proteomics with other omics methods creates a powerful tool that can expand our understanding about diverse probiotic functionality. In this review, proteomic strategies for identification/quantitation of the proteins in probiotic bacteria were overviewed. The types of probiotic proteins investigated by proteomics were described, such as intracellular proteins, surface proteins, secreted proteins, and the proteins of extracellular vesicles. Examples of pathological conditions in which probiotic bacteria played crucial roles were discussed.
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Tullio, Vivian. „Probiotic Yeasts: A Developing Reality?“ Journal of Fungi 10, Nr. 7 (16.07.2024): 489. http://dx.doi.org/10.3390/jof10070489.
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Yeasts are gaining increasing attention for their potential health benefits as probiotics in recent years. Researchers are actively searching for new yeast strains with probiotic properties (i.e, Debaryomyces hansenii; Kluyveromyces marxianus; Yarrowia lipolytica; Pichia hudriavzevii; and Torulaspora delbrueckii) from various sources, including traditional fermented foods, the human gut, and the environment. This exploration is expanding the pool of potential probiotic yeasts beyond the well-studied Saccharomyces boulardii. Research suggests that specific yeast strains possess properties that could be beneficial for managing conditions like inflammatory bowel disease, irritable bowel syndrome, skin disorders, and allergies. Additionally, probiotic yeasts may compete with pathogenic bacteria for adhesion sites and nutrients, thereby inhibiting their growth and colonization. They might also produce antimicrobial compounds that directly eliminate harmful bacteria. To achieve these goals, the approach that uses probiotics for human health is changing. Next-generation yeast probiotics are emerging as a powerful new approach in the field of live biotherapeutics. By using genetic engineering, scientists are able to equip these tools with specialized capabilities. However, most research on these probiotic yeasts is still in its early stages, and more clinical trials are needed to confirm their efficacy and safety for various health conditions. This review could provide a brief overview of the situation in this field.
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He, Xiaoya, Shuyang Zhao und Yan Li. „Faecalibacterium prausnitzii: A Next-Generation Probiotic in Gut Disease Improvement“. Canadian Journal of Infectious Diseases and Medical Microbiology 2021 (05.03.2021): 1–10. http://dx.doi.org/10.1155/2021/6666114.
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The researchers are paying more attention to the role of gut commensal bacteria in health development beyond the classical pathogens. It has been widely demonstrated that dysbiosis, which means the alternations of the gut microbial structure, is closely associated with development of intestinal chronic inflammation-related diseases such as inflammatory bowel disease (IBD), and even infectious diseases including bacterial and viral infection. Thus, for reshaping ecological balance, a growing body of the literatures have proposed numerous strategies to modulate the structure of the gut microbiota, which provide more revelation for amelioration of these inflammation or infection-related diseases. While the ameliorative effects of traditional probiotics seem negligeable, emerging next generation probiotics (NGPs) start to receive great attention as new preventive and therapeutic tools. Encouragingly, within the last decade, the intestinal symbiotic bacterium Faecalibacterium prausnitzii has emerged as the “sentinel of the gut,” with multifunction of anti-inflammation, gut barrier enhancement, and butyrate production. A lower abundance of F. prausnitzii has been shown in IBD, Clostridium difficile infection (CDI), and virus infection such as COVID-19. It is reported that intervention with higher richness of F. prausnitzii through dietary modulation, fecal microbiota transplantation, or culture strategy can protect the mice or the subjects from inflammatory diseases. Therefore, F. prausnitzii may have potential ability to reduce microbial translocation and inflammation, preventing occurrences of gastrointestinal comorbidities especially in COVID-19 patients.
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Ullah, Mati, Ali Raza, Li Ye und Zhu Yu. „Viability and Composition Validation of Commercial Probiotic Products by Selective Culturing Combined with Next-Generation Sequencing“. Microorganisms 7, Nr. 7 (29.06.2019): 188. http://dx.doi.org/10.3390/microorganisms7070188.
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The consumption of dietary supplements to treat health complications or to improve overall health conditions has become a globally increasing trend that leads to the development of a large number of health-related novel products and expands the associated manufacturing industries around the world. In the current study, we applied selective culturing combined with next-generation sequencing to examine the microbial viability in terms of its culturability on culture medium, composition, and possible contamination in the selected 17 commercial probiotic products sold in the mainland China market. Additionally, the relative abundance of each individual bacterial content was also evaluated by using the generated sequencing reads. The tested probiotic product samples were subjected to Illumina HiSeq-2000 sequencing platform and thoroughly analyzed by the in-house developed bioinformatics pipeline. The comprehensive culturing and sequencing analysis revealed both viability and composition inaccuracy among the several tested probiotic products, however, no contaminant was identified during the analysis. Among the total, five probiotic products (29.41%) were found with an inaccurate or lower colony-forming unit (CFU) counts on culture media while four probiotic products (23.52%) have inaccurately labeled classification. This study provides an ideal qualitative and quantitative assessment approach, which can be used as a diagnostic tool for the accurate assessment of commercial probiotic supplements.
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Hizo, Gabriel Henrique, und Pabulo Henrique Rampelotto. „The Impact of Probiotic Bifidobacterium on Liver Diseases and the Microbiota“. Life 14, Nr. 2 (08.02.2024): 239. http://dx.doi.org/10.3390/life14020239.
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Recent studies have shown the promising potential of probiotics, especially the bacterial genus Bifidobacterium, in the treatment of liver diseases. In this work, a systematic review was conducted, with a focus on studies that employed advanced Next Generation Sequencing (NGS) technologies to explore the potential of Bifidobacterium as a probiotic for treating liver pathologies such as Non-Alcoholic Fatty Liver Disease (NAFLD), Non-Alcoholic Steatohepatitis (NASH), Alcoholic Liver Disease (ALD), Cirrhosis, and Hepatocelullar Carcinoma (HCC) and its impact on the microbiota. Our results indicate that Bifidobacterium is a safe and effective probiotic for treating liver lesions. It successfully restored balance to the intestinal microbiota and improved biochemical and clinical parameters in NAFLD, ALD, and Cirrhosis. No significant adverse effects were identified. While more research is needed to establish its efficacy in treating NASH and HCC, the evidence suggests that Bifidobacterium is a promising probiotic for managing liver lesions.
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Abdelghani, Dina Y., Abdallah I. Gad, Mona M. Orabi, Khadiga A. Abou-Taleb, Emam A. Mahmoud, Soha A. Al Amoudi, Ali Zari, Eman Hillal Althubaiti, Sherif Edris und Shimaa A. Amin. „Bioactivity of Organic Fermented Soymilk as Next-Generation Prebiotic/Probiotics Mixture“. Fermentation 8, Nr. 10 (04.10.2022): 513. http://dx.doi.org/10.3390/fermentation8100513.
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Fermented soymilk (soymilk yogurt) was made by fermenting soymilk with five probiotic bacterial strains (Lactobacillus plantarum ATCC 14917, Lactobacillus casei DSM 20011, Lactobacillus acidophilus ATCC 20552, Lactococcus thermophilus DSM 20259, and Bifidobacterium longum B41409) that were used as monocultures and combined with them as consortia cultures. Seven pathogenic strains, E. coli O157H7, S. aureus As4, S. typhimurium As3, S. shigae As2, L. monocytogenes As1, P. aeruginosa ATCC 27853, and B. cereus Dsmz 345, were used to study the antibacterial activity of fermented soymilk by agar well diffusion assay. Results indicated that Gram-negative pathogenesis was more sensitive to probiotic cultures than Gram-positive pathogenesis. E. coli O15H7, S. typhimirium As3, and Shigella shigae As2 were more sensitive to probiotic cultures, presenting inhibition zone diameters (IZA) ranging from 10 to 20 mm, 12 to 16 mm, and 10 to 16 mm, respectively. At the same time, P. aeruginosa Atcc 27853 showed the lowest (IZA), ranging from 3 mm to 8 mm. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined at various concentrations of soymilk fermented by T1, T4, and T5, ranging from 0.031 mg/mL to 1 mg/mL against pathogenic bacterial strains. The sensory properties of FSM were evaluated, and sensory analysis during soymilk fermentation showed significant improvement. The effect of shelf life (storage period) on FSM quality and properties was evaluated; during shelf life (storage period), FSM saved its properties and quality after 28 days of cold storage. Finally, it was stated that the soymilk yogurt can be used as a substitute for buffalo and cow milk for therapeutic feeding in the future.
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Marcial-Coba, Martín Sebastián, Susanne Knøchel und Dennis Sandris Nielsen. „Low-moisture food matrices as probiotic carriers“. FEMS Microbiology Letters 366, Supplement_1 (09.01.2019): i49—i59. http://dx.doi.org/10.1093/femsle/fnz006s.
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ABSTRACT To exert a beneficial effect on the host, adequate doses of probiotics must be administered and maintaining their viability until consumption is thus essential. Dehydrated probiotics exhibit enhanced long-term viability and can be incorporated into low-moisture food matrices, which also possess high stability at refrigeration and ambient temperature. However, several factors associated with the desiccation process, the physicochemical properties of the matrix and the storage conditions can affect probiotic survival. In the near future, an increased demand for probiotics based on functionally dominant members of the gut microbiome (‘next-generation probiotics’, NGP) is expected. NGPs are very sensitive to oxygen and efficient encapsulation protocols are needed. Strategies to improve the viability of traditional probiotics and particularly of NGPs involve the selection of a suitable carrier as well as proper desiccation and protection techniques. Dehydrated probiotic microcapsules may constitute an alternative to improve the microbial viability during not only storage but also upper gastrointestinal tract passage. Here we review the main dehydration techniques that are applied in the industry as well as the potential stresses associated with the desiccation process and storage. Finally, low- or intermediate-moisture food matrices suitable as carriers of traditional as well as NGPs will be discussed.
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Machado, Patrícia, Felipe Neme Ribeiro, Fernanda Cristina Wroblevski Giublin, Naomi Gerzvolf Mieres, Fernanda Stumpf Tonin, Roberto Pontarolo, Marcel Henrique Marcondes Sari, Raul Edison Luna Lazo und Luana Mota Ferreira. „Next-Generation Wound Care: A Scoping Review on Probiotic, Prebiotic, Synbiotic, and Postbiotic Cutaneous Formulations“. Pharmaceuticals 18, Nr. 5 (09.05.2025): 704. https://doi.org/10.3390/ph18050704.
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Background/Objectives: Chronic wounds represent a significant socioeconomic burden, affecting 1–2% of the global population. Wound healing is a complex process involving inflammation, cell proliferation, and tissue remodeling, but factors such as infections, diabetes, aging, and poor nutrition can impair recovery, leading to chronic wounds. Given these challenges, researchers have explored topical probiotics, synbiotics, and postbiotics as alternatives strategies. Strains like Lactobacillus and Bifidobacterium contribute to skin restoration by producing antimicrobial, anti-inflammatory, and immunomodulatory compounds, offering a novel approach to cutaneous restoration. Our study aims to address the potential effects of topical probiotic, synbiotic, and postbiotic formulations for wound healing applications by means of a broad scoping review and evidence-gap mapping. Methods: A systematic literature search of preclinical studies (in vitro and in vivo) was performed in PubMed, Scopus, and Web of Science (January 2025), yielding 3052 articles after duplicates removal, of which 44 met the inclusion criteria. Results: These studies were published between 1986 and 2024, mostly by China (27.3%) and Iran (25.0%). Probiotics were frequently evaluated among the studies included (47.7%) (with Lactobacillus plantarum being the most assessed strain), followed by postbiotics (36.4%) (with predominant use of cell-free supernatants) and synbiotics (15.9%) (especially fructooligosaccharides). Dosage forms included gels (44.4%), films (15.6%), and ointments (13.3%). Conclusions: Most studies indicate that probiotics, synbiotics, and postbiotics have antimicrobial and anti-inflammatory properties, while promoting angiogenesis, tissue regeneration, and skin barrier restoration. The use of different delivery systems may additionally enhance therapeutic outcomes by accelerating wound closure, reducing bacterial load, and modulating immune response. However, methodological limitations in animal studies highlight the need for greater experimental rigor. Further robust clinical trials are essential to confirm efficacy and safety before clinical application of these formulations.
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Nurashila, Alin Shelina, Yuniar Mulyani, Zuzy Anna und Mochamad Untung Kurnia Agung. „ANALYSIS OF BACTERIAL ABUNDANCE IN THE INTESTINES OF COMMON CARP Cyprinus carpio TREATED WITH THE PROBIOTIC Bacillus subtilis“. AQUASAINS 11, Nr. 2 (29.04.2023): 1269. http://dx.doi.org/10.23960/aqs.v11i2.p1269-1276.
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Efforts to increase microbial populations in fish intestines tract can be done by utilizing microbes in the fish's digestive tract as probiotics. The application of Bacillus subtilis in fish feed mixtures can provide better growth performance in modifying microbes in the intestines of fish. The aim of this research was to analyze the bacterial community in the intestines of Common Carp(Cyprinus carpio) that have been given probiotics from the bacteria Bacillus subtilis with metagenome sequencing using 16S rRNA through Next Generation Sequencing (NGS) technology. Common carp samples were collected from maintenance conducted with different treatments, namely control fish without probiotic administration and fish treatment with mixed feed probiotic Bacillus subtilis. This research was conducted in the Laboratory of Fisheries Biotechnology, Faculty of Fisheries and Marine Science, Padjajaran University. Then molecular identification was carried out using Illumina's NGS in Novogene, Singapore. The results of the identification of using probiotic bacteria Bacillus subtilis in Common carp(Cyprinus carpio) feed affect the bacterial community in the fish intestine. In the intestines of carp that were given probiotics, the highest abundance was Proteobacteria, Fusobacteriota, and firmicutes.The highest abundance at the genus level in both samples was domiciled by the genus Cetobacterium followed by the Sphingomonas and Aeromonas.
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Abouelela, Mohamed E., und Yosra A. Helmy. „Next-Generation Probiotics as Novel Therapeutics for Improving Human Health: Current Trends and Future Perspectives“. Microorganisms 12, Nr. 3 (20.02.2024): 430. http://dx.doi.org/10.3390/microorganisms12030430.
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Next-generation probiotics (NGPs) represent an innovative group of beneficial bacteria that are currently undergoing research and development. NGPs are designed not only for conventional use as foods or dietary supplements but are also tailored for pharmaceutical applications. Research indicates that NGPs show therapeutic promise in addressing various chronic ailments. Offering multiple advantages over conventional probiotics, NGPs present opportunities for personalized probiotic therapies, involvement in synthetic biology and gene editing, participation in combination therapies, targeted delivery methods, and application in therapeutic settings. Our review discusses the potential therapeutic effect of the NGPs, covering diverse research trajectories for NGPs, including their identification, characterization, and targeted delivery. Furthermore, this review elucidates the influence of NGPs on critical aspects of human health, specifically, gut health, immune function, and broader health outcomes. Mechanistic insights encompass the production of bioactive compounds, competitive interactions with pathogenic bacteria, the modulation of immune cell activity, and the reinforcement of the gut barrier. What is noteworthy is that the current review points out the prevalent NGP strains and their diverse sources, providing a highlight for the comprehensive framework for understanding their potential applications and their future benefits in the domain of advanced therapeutics.
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Lee, Hak-Jae, Do-Won Ham, Seung-Hwan Seo, Guang-Ho Cha und Eun-Hee Shin. „Probiotic-induced changes in intestinal microbiome inhibits Toxoplasma gondii infection“. Parasites, Hosts and Diseases 62, Nr. 4 (22.11.2024): 408–23. http://dx.doi.org/10.3347/phd.24068.
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Toxoplasma gondii primarily invades the central nervous system, causing latent infections. Cysts persist in the host for life and there is currently no effective treatment. T. gondii infects human hosts through contaminated meat, invading the intestinal tissue and leading to changes in the number and composition of the gut microbiota. Since probiotic ingestion modulates intestinal microbiota changes, we hypothesized that intestinal microbiota dysbiosis caused by T. gondii infection would be restored following probiotic supplementation. To this end, we orally infected C57BL/6 mice with 10 T. gondii cysts and administered supplemental probiotics daily. We analyzed the levels of T. gondii B1 gene DNA, indicative of T. gondii infection, in brain tissue. We investigated alterations in the gut microbiota composition and functional pathways between the probiotic and non-probiotic treatment groups via next-generation sequencing analysis of each fecal sample. The infection level in the probiotic-treated group was significantly reduced after 4 weeks (p<0.05). Probiotic supplementation notably changed the gut microbiota after 2 weeks of infection, increasing the relative abundance of Intestinimonas massiliensis and Lawsonibacter asaccharolyticus. Probiotic supplements appear to modulate the gut microbiota, activating functional pathways involved in intestinal short-chain fatty acid production and strengthening the intestinal barrier, thereby impeding T. gondii infection and subsequent proliferation. Our findings provide valuable insights into T. gondii infection control and future study directions.
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Chuang, Shih-Te, Chien-Ting Chen, Jui-Chun Hsieh, Kuan-Yi Li, Shang-Tse Ho und Ming-Ju Chen. „Development of Next-Generation Probiotics by Investigating the Interrelationships between Gastrointestinal Microbiota and Diarrhea in Preruminant Holstein Calves“. Animals 12, Nr. 6 (10.03.2022): 695. http://dx.doi.org/10.3390/ani12060695.
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(1) Background: We aimed to isolate and identify potential next-generation probiotics (NGP) by investigating the interrelationships between gastrointestinal microbiota and diarrhea in preruminant Holstein calves. (2) Material and methods: Twenty preruminant Holstein calves were divided into healthy and diarrheic groups after the combination outcomes of veterinary diagnosis and fecal scores. The fecal microbiome, plasma cytokines, plasma immunoglobulin (Ig) G and haptoglobin were analyzed. The potential probiotic bacteria were identified by comparing the microbiota difference between healthy and diarrheic calves and correlation analysis with fecal scores and inflammatory markers. The identified bacteria were also isolated for further evaluation for antimicrobial activities and immunoregulatory effects. (3) Results: Microbiota analysis suggested that Ruminococcaceae_UCG_014, Bifidobacterium and Pseudoflavonifractor positively correlated with bovine IgG and negatively correlated with fecal score; inflammatory factors, bovine HP, and IL-8 were classified as beneficial bacteria contributing to the health of the calves. The alternation of gut microbial composition also induced changes in the functional gene enrichment of gut microbiota in calves. The gathering of microbiomic data strongly indicated the possible beneficial effects of Bifidobacterium longum subsp. longum, expected to develop as NGP. After isolation and evaluation of the potential functionality in vitro, two specific bifidobacterial strains demonstrated antimicrobial activities and immunoregulatory effects. (4) Conclusions: The results provide a new probiotic searching approach for preventing gastrointestinal disorders in preruminant calves. Further animal study is necessary to verify the results.
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Kaźmierczak-Siedlecka, Karolina, Giandomenico Roviello, Martina Catalano und Karol Polom. „Gut Microbiota Modulation in the Context of Immune-Related Aspects of Lactobacillus spp. and Bifidobacterium spp. in Gastrointestinal Cancers“. Nutrients 13, Nr. 8 (31.07.2021): 2674. http://dx.doi.org/10.3390/nu13082674.
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Accumulating evidence has revealed the critical roles of commensal microbes in cancer progression and recently several investigators have evaluated the therapeutic effectiveness of targeting the microbiota. This gut microbiota-related approach is especially attractive in the treatment of gastrointestinal cancers. Probiotics supplementation is a microbiota-targeted strategy that appears to improve treatment efficacy; Lactobacillus spp. and Bifidobacterium spp. are among the most commonly used probiotic agents. These bacteria seem to exert immunomodulatory effects, impacting on the immune system both locally and systemically. The gut microbiota are able to affect the efficiency of immunotherapy, mainly acting as inhibitors at immune checkpoints. The effects of immunotherapy may be modulated using traditional probiotic strains and/or next generation probiotics, such as Akkermansia municiphila. It is possible that probiotics might enhance the efficiency of immunotherapy based on PD-1/PD-L1 and CTLA-4 but more data are needed to confirm this speculation. Indeed, although there is experimental evidence for the efficacy of several strains, the health-promoting effects of numerous probiotics have not been demonstrated in human patients and furthermore the potential risks of these products, particularly in oncologic patients, are rarely mentioned.
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Lin, Tzu-Lung, Cha-Chen Lu, Ting-Wen Chen, Chih-Wei Huang, Jang-Jih Lu, Wei-Fan Lai, Ting-Shu Wu, Chih-Ho Lai, Hsin-Chih Lai und Ya-Lei Chen. „Amelioration of Maternal Immune Activation-Induced Autism Relevant Behaviors by Gut Commensal Parabacteroides goldsteinii“. International Journal of Molecular Sciences 23, Nr. 21 (28.10.2022): 13070. http://dx.doi.org/10.3390/ijms232113070.
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Autism spectrum disorder (ASD) is characterized by cognitive inflexibility and social deficits. Probiotics have been demonstrated to play a promising role in managing the severity of ASD. However, there are no effective probiotics for clinical use. Identifying new probiotic strains for ameliorating ASD is therefore essential. Using the maternal immune activation (MIA)-based offspring ASD-like mouse model, a probiotic-based intervention strategy was examined in female mice. The gut commensal microbe Parabacteroides goldsteinii MTS01, which was previously demonstrated to exert multiple beneficial effects on chronic inflammation-related-diseases, was evaluated. Prenatal lipopolysaccharide (LPS) exposure induced leaky gut-related inflammatory phenotypes in the colon, increased LPS activity in sera, and induced autistic-like behaviors in offspring mice. By contrast, P. goldsteinii MTS01 treatment significantly reduced intestinal and systemic inflammation and ameliorated disease development. Transcriptomic analyses of MIA offspring indicated that in the intestine, P. goldsteinii MTS01 enhanced neuropeptide-related signaling and suppressed aberrant cell proliferation and inflammatory responses. In the hippocampus, P. goldsteinii MTS01 increased ribosomal/mitochondrial and antioxidant activities and decreased glutamate receptor signaling. Together, significant ameliorative effects of P. goldsteinii MTS01 on ASD relevant behaviors in MIA offspring were identified. Therefore, P. goldsteinii MTS01 could be developed as a next-generation probiotic for ameliorating ASD.
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Machado, Daniela, Joana Cristina Barbosa, Diana Almeida, José Carlos Andrade, Ana Cristina Freitas und Ana Maria Gomes. „Insights into the Antimicrobial Resistance Profile of a Next Generation Probiotic Akkermansia muciniphila DSM 22959“. International Journal of Environmental Research and Public Health 19, Nr. 15 (27.07.2022): 9152. http://dx.doi.org/10.3390/ijerph19159152.
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Akkermansia muciniphila is a Gram-negative intestinal anaerobic bacterium recently proposed as a novel probiotic candidate to be incorporated in food and pharmaceutical forms. Despite its multiple health benefits, the data addressing its antimicrobial susceptibility profile remain scarce. However, the absence of acquired resistance in probiotic strains is a compulsory criterion for its approval in the qualified presumption of safety list. This study aimed at characterizing the A. muciniphila DSM 22959 strain’s antimicrobial susceptibility profile using phenotypic and in silico approaches. To establish the phenotypic antimicrobial susceptibility profile of this strain, minimum inhibitory concentrations of eight antimicrobials were determined using broth microdilution and E-test methods. Additionally, the A. muciniphila DSM 22959 genome was screened using available databases and bioinformatics tools to identify putative antimicrobial resistance genes (ARG), virulence factors (VF), genomic islands (GI), and mobile genetic elements (MGE). The same categorization was obtained for both phenotypic methods. Resistance phenotype was observed for gentamicin, kanamycin, streptomycin, and ciprofloxacin, which was supported by the genomic context. No evidence was found of horizontal acquisition or potential transferability of the identified ARG and VF. Thus, this study provides new insights regarding the phenotypic and genotypic antimicrobial susceptibility profiles of the probiotic candidate A. muciniphila DSM 22959.
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Singer, Georg, Karl Kashofer, Christoph Castellani und Holger Till. „Hirschsprung’s Associated Enterocolitis (HAEC) Personalized Treatment with Probiotics Based on Gene Sequencing Analysis of the Fecal Microbiome“. Case Reports in Pediatrics 2018 (14.10.2018): 1–6. http://dx.doi.org/10.1155/2018/3292309.
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Approximately 40% of children with Hirschsprung’s disease (HD) suffer from Hirschsprung’s associated enterocolitis (HAEC) despite correct surgery. Disturbances of the intestinal microbiome may play a role. Treatment with probiotics based on individual analyses of the fecal microbiome has not been published for HD patients with recurrent HAEC yet. A boy with trisomy 21 received transanal pull-through at the age of 6 months for rectosigmoid HD. With four years, he suffered from recurrent episodes of HAEC. The fecal microbiome was measured during three healthy and three HAEC episodes by next-generation sequencing. The patient was started on daily probiotics for 3 months; the fecal microbiome was measured weekly. The fecal microbiome differed significantly between healthy and HAEC episodes. HAEC episodes were associated with significant decreases of Actinobacteria and significant increases of Bacteroidetes and Proteobacteria. Probiotic treatment led to a significant increase of alpha diversity and a significant increase of Bifidobacterium and Streptococcus as well as decreases of Rikenellaceae, Pseudobutyrivibrio, Blautia, and Lachnospiraceae. A longitudinal observation of the microbiome has never been performed following correction of Hirschsprung’s disease. Probiotic treatment significantly changed the fecal microbiome; the alterations were not limited to strains contained in the administered probiotics.
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Pető, Ágota, Dóra Kósa, Zoltán Szilvássy, Pálma Fehér, Zoltán Ujhelyi, Gabriella Kovács, István Német, István Pócsi und Ildikó Bácskay. „Scientific and Pharmaceutical Aspects of Christensenella minuta, a Promising Next-Generation Probiotic“. Fermentation 9, Nr. 8 (17.08.2023): 767. http://dx.doi.org/10.3390/fermentation9080767.
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Christensenella minuta (C. minuta), a member of a recently described bacterial family, is one of the most heritable next-generation probiotics. Many observational studies confirmed that the relative abundance of C. minuta is associated with lean body types with a low host body mass index (BMI), and is also influenced by age, diet, and genetics. By utilizing its benefits, it could be suited to many therapies, including human and animal health as well. However, a reliable method for culturing the strain must also be developed to enable the therapeutic administration of the microbe. Sludge microfiltration could be a promising solution for large scale-up cultivation. In this review, different processing methods are also described from pharmaceutical aspects.
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He, Siqin, Simin Lu, Yuanming Huang, Liqiong Song, Tao Yang, Yuchun Xiao und Zhihong Ren. „Next-generation probiotic Bacteroides. dorei: Improving the efficacy of COVID-19 vaccinations“. Journal of Functional Foods 116 (Mai 2024): 106190. http://dx.doi.org/10.1016/j.jff.2024.106190.
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Bahuguna, Nikita, Jigisha Anand, Divya Venugopal, Ankit Khanduri und Nishant Rai. „Biotherapeutic potentials of diverse probiotics of the Himalayan region: A Review“. Environment Conservation Journal 26, Nr. 1 (10.01.2025): 238–48. https://doi.org/10.36953/ecj.30203155.
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The highest ecosystems on Earth are located in India's Himalayan region, covering a distance of 3500 km from Jammu and Kashmir to Arunachal Pradesh in the northeastern part of the country. Limited information on the probiotic diversity within the traditional diets of the Western Himalaya is available. Despite living in the challenging environment of high altitudes, the inhabitants of these areas display healthy and long lifespans, which are primarily attributed to their diet, particularly the traditional fermented foods. The potential for isolating native probiotic bacteria from traditional foods is, therefore, enormous. One of the main strategies for preventative treatment in humans may be to supplement the diet with nutrients that can shield against immune system problems and lower the risk of contracting illnesses like mycosis. The emergence of functional foods and nutraceuticals underscores the importance of reducing reliance on medications and increasing regular consumption of fermented foods. Researchers primarily suggest probiotics and lactic acid bacteria (LAB) as nutraceuticals due to their non-harmful effects on human health, their ability to activate the immune system, and their ability to enhance resistance to various illness situations. As per WHO, probiotic bacteria are defined as “live microorganisms that, when administered in adequate amounts, confer a health benefit on the host.” Antifungal agents like azoles and amphotericin B are recommended as therapeutic agents as per the case. However, the emergence of drug-resistant strains worldwide has reduced the efficacy of these treatments. According to health organizations (national and international, including WHO), there is an urgent need to explore novel and alternative options, including probiotics. The current review highlights the biotherapeutic perspective of diverse probiotic strains, including next-generation probiotics against mycosis.
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Emanowicz, Paulina, Paulina Średnicka, Michał Wójcicki, Marek Roszko und Edyta Juszczuk-Kubiak. „Mitigating Dietary Bisphenol Exposure Through the Gut Microbiota: The Role of Next-Generation Probiotics in Bacterial Detoxification“. Nutrients 16, Nr. 21 (31.10.2024): 3757. http://dx.doi.org/10.3390/nu16213757.
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Bisphenols, such as bisphenol A and its analogs, which include bisphenol S, bisphenol F, bisphenol AF, and tetramethyl bisphenol F, are chemical contaminants commonly found in food that raise serious health concerns. These xenobiotics can potentially have harmful effects on human health. The gut microbiota plays a crucial role in metabolizing and neutralizing these substances, which is essential for their detoxification and elimination. Probiotic supplementation has been studied for its ability to modulate the gut microbiota’s composition and function, enhancing detoxification processes. Next-Generation Probiotics (NGPs) may exhibit better properties than traditional strains and are designed for targeted action on specific conditions, such as obesity. By modulating inflammatory responses and reducing the secretion of pro-inflammatory cytokines, they can significantly improve host health. Research on NGPs’ ability to neutralize obesogenic bisphenols remains limited, but their potential makes this a promising area for future exploration. This review aims to understand the mechanisms of the chemical transformation of bisphenol through its interactions with the gut microbiota and the role of probiotics, particularly NGPs, in these processes. Understanding the interplay between bisphenols, gut microbiota, and NGPs may pave the way for strategies to counteract the negative health effects associated with daily and chronic exposure to bisphenols, which is crucial for food safety and consumer health protection.
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Canaviri-Paz, Pamela, Elin Oscarsson, Anna Kjellström, Hanna Olsson, Chandana Jois und Åsa Håkansson. „Effects on Microbiota Composition after Consumption of Quinoa Beverage Fermented by a Novel Xylose-Metabolizing L. plantarum Strain“. Nutrients 13, Nr. 10 (23.09.2021): 3318. http://dx.doi.org/10.3390/nu13103318.
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Demands for novel lactic acid bacteria with potential to be used as probiotics along with healthy fermented plant-based products increase worldwide. In this study, a novel Lactiplantibacillus plantarum P31891 strain with enzymatic capacity to degrade tannins and ferment xylose was used as starter culture for fermentation of a quinoa-based beverage. The probiotic potential of the selected strain was evaluated in healthy volunteers. Twenty participants consumed the beverage for 14 days; microbiota changes in saliva and faecal samples were analyzed by Terminal Restriction Fragment Length Polymorphism (T-RFLP), Next Generation Sequencing (NGS) and qPCR; and gastrointestinal well-being and digestive symptoms were recorded. The results indicated that the consumption of the beverage with Lactiplantibacillus plantarum P31891 in a probiotic dose (1012 CFU/mL) increased the number of Lactobacillus in the feces but not in saliva. Overall, the bacterial community did not seem to be influenced by the bacterium or by the beverage, as expressed by the diversity indexes, but specific genera were affected, as reflected in changes in amplicon sequence variants. Consequently, Lactiplantibacillus plantarum P31891 showed potential to be categorized as a probiotic strain in the fermented quinoa-based beverage.
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Ma, Changlu, Qichen Liu, Shuwen Zhang, Ailing Qu, Qing Liu, Jiaping Lv und Xiaoyang Pang. „Lactobacillus Kefir M20 Adaptation to Bile Salts: A Novel Pathway for Cholesterol Reduction“. Foods 13, Nr. 21 (24.10.2024): 3380. http://dx.doi.org/10.3390/foods13213380.
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(1) Background: This study investigated the impact of in vitro adaptations to acid and bile stress on the cholesterol-lowering activity of the probiotic Lactobacillus kefir M20. (2) Methods: Lactobacillus kefir M20 was extracted from fermented dairy products in Xinjiang, China, and isolated using MRS medium. The lactic acid bacteria were cultured for stress resistance to acid and bile salts and then gavaged into mice for animal experiments. (3) Results: The adaptation to bile stress treatment resulted in a notable enhancement of the cholesterol-lowering capacity of Lactobacillus kefir M20, with reductions of 16.5% and 33.1% in total and non-HDL cholesterol, respectively, compared to the untreated strain. Furthermore, the daily fecal total bile acid excretion was 9.2, 5.4 and 5.0 times higher in the M20-BSA group compared to the HC, M20 and M20-ASA groups, respectively. (4) Conclusions: This study suggests that targeted probiotics have the potential for application in the next generation of functional foods and probiotic formulations aimed at combating hypercholesterolemia.
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Novotny-Nuñez, Ivanna, Gabriela Perdigón, Chantal Matar, María José Martínez Monteros, Nour Yahfoufi, Silvia Inés Cazorla und Carolina Maldonado-Galdeano. „Evaluation of Rouxiella badensis Subsp Acadiensis (Canan SV-53) as a Potential Probiotic Bacterium“. Microorganisms 11, Nr. 5 (20.05.2023): 1347. http://dx.doi.org/10.3390/microorganisms11051347.
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The advent of omic platforms revealed the significant benefits of probiotics in the prevention of many infectious diseases. This led to a growing interest in novel strains of probiotics endowed with health characteristics related to microbiome and immune modulation. Therefore, autochthonous bacteria in plant ecosystems might offer a good source for novel next-generation probiotics. The main objective of this study was to analyze the effect of Rouxiella badensis acadiensis Canan (R. acadiensis) a bacterium isolated from the blueberry biota, on the mammalian intestinal ecosystem and its potential as a probiotic microorganism. R. acadiensis, reinforced the intestinal epithelial barrier avoiding bacterial translocation from the gut to deep tissues, even after feeding BALB/c mice for a prolonged period of time. Moreover, diet supplementation with R. acadiensis led to increases in the number of Paneth cells, well as an increase in the antimicrobial peptide α defensin. The anti-bacterial effect of R. acadiensis against Staphylococcus aureus and Salmonella enterica serovar Typhimurium was also reported. Importantly, R. acadiensis-fed animals showed better survival in an in vivo Salmonella enterica serovar Typhimurium challenge compared with those that received a conventional diet. These results demonstrated that R. acadiensis possesses characteristics of a probiotic strain by contributing to the reinforcement and maintenance of intestinal homeostasis.
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Jiang, Tianyi, Huan Wu, Xin Yang, Yue Li, Ziyi Zhang, Feng Chen, Liping Zhao und Chenhong Zhang. „Lactobacillus Mucosae Strain Promoted by a High-Fiber Diet in Genetic Obese Child Alleviates Lipid Metabolism and Modifies Gut Microbiota in ApoE-/- Mice on a Western Diet“. Microorganisms 8, Nr. 8 (12.08.2020): 1225. http://dx.doi.org/10.3390/microorganisms8081225.
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Supplementation of probiotics is a promising gut microbiota-targeted therapeutic method for hyperlipidemia and atherosclerosis. However, the selection of probiotic candidate strains is still empirical. Here, we obtained a human-derived strain, Lactobacillus mucosae A1, which was shown by metagenomic analysis to be promoted by a high-fiber diet and associated with the amelioration of host hyperlipidemia, and validated its effect on treating hyperlipidemia and atherosclerosis as well as changing structure of gut microbiota in ApoE-/- mice on a Western diet. L. mucosae A1 attenuated the severe lipid accumulation in serum, liver and aortic sinus of ApoE-/- mice on a Western diet, while it also reduced the serum lipopolysaccharide-binding protein content of mice, reflecting the improved metabolic endotoxemia. In addition, L. mucosae A1 shifted the gut microbiota structure of ApoE-/- mice on a Western diet, including recovering a few members of gut microbiota enhanced by the Western diet. This study not only suggests the potential of L. mucosae A1 to be a probiotic in the treatment of hyperlipidemia and atherosclerosis, but also highlights the advantage of such function-based rather than taxonomy-based strategies for the selection of candidate strains for the next generation probiotics.
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Liu, Qitong, Shu Liu, Qinwen Ye, Xiaoyue Hou, Guang Yang, Jing Lu, Yang Hai, Juan Shen und Yaowei Fang. „A Novel Streptococcus thermophilus FUA329 Isolated from Human Breast Milk Capable of Producing Urolithin A from Ellagic Acid“. Foods 11, Nr. 20 (20.10.2022): 3280. http://dx.doi.org/10.3390/foods11203280.
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Urolithin A, a metabolite of ellagic acid, has many beneficial biological activities for people. Strains capable of producing urolithin A from ellagic acid have the hope of becoming the next-generation probiotics. However, only a few species of these strains have been reported. In this study, FUA329, a strain capable of converting ellagic acid to urolithin A in vitro, was isolated from the breast milk of healthy Chinese women. The results of morphological observation, physiological and biochemical tests, and 16S rRNA gene sequence analysis confirmed that the strain FUA329 was Streptococcus thermophilus. In addition, the S. thermophilus FUA329 growth phase is consistent with the degradation of ellagic acid, and urolithin A was produced in the stationary phase, with a maximum concentration of 7.38 μM at 50 h. The corresponding conversion efficiency of urolithin A from ellagic acid was 82%. In summary, S. thermophilus FUA329, a novel urolithin A-producing bacterium, would be useful for the industrial production of urolithin A and may be developed as a next-generation probiotic.
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Mikhailova, Natalia A., Dmitry A. Voevodin und Sergey A. Lazarev. „A modern view of pro-/eukaryote interactions in the human body as the basis for development of next-generation probiotics“. Journal of microbiology, epidemiology and immunobiology 97, Nr. 4 (02.09.2020): 346–55. http://dx.doi.org/10.36233/0372-9311-2020-97-4-7.
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Multicellular organisms and the saprophytic flora form complex, highly integrated chimeric systems (associative symbioses, metaorganisms) characterized by interplay between pro- and eukaryotic components. To be able to interact symbiotically microorganisms (MO) need a whole body.When grown on artificial media for a long time, symbiotic MO have to adapt to the artificial environment and gradually, though reversibly, lose their ability of associative interaction with the human body, thus causing a decrease in the therapeutic efficacy of MO-derived probiotic products. To increase the therapeutic activity of probiotic MO, they must be functionally rehabilitated.A pathological process induces development of a secondary metabolic dysbiosis; as a result, changes in the regulatory processes of an individual interfere with the restoration of the normal microflora. Therefore, functional rehabilitation of probiotic MO must take place during cultivation, while the cultivation process must replicate the whole-body conditions.
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Lai, Hung-Hsiang, Cheng-Hsun Chiu, Man-Shan Kong, Chee-Jen Chang und Chien-Chang Chen. „Probiotic Lactobacillus casei: Effective for Managing Childhood Diarrhea by Altering Gut Microbiota and Attenuating Fecal Inflammatory Markers“. Nutrients 11, Nr. 5 (23.05.2019): 1150. http://dx.doi.org/10.3390/nu11051150.
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Background: Acute diarrhea is a major cause of childhood morbidity and an economic burden for families. The aim of this study is to explore the effect of probiotics on clinical symptoms, intestinal microbiota, and inflammatory markers during childhood diarrhea. Methods: Children (n = 81) aged six months to six years (mean age 2.31 years) hospitalized for acute diarrhea were randomized to receive probiotics (Lactobacillus casei variety rhamnosus; n = 42) or no probiotics (n = 39) orally twice daily for seven days. Feces samples were also collected to evaluate microbial content using a traditional agar plate and next-generation sequencing. Immunoglobulin A (IgA), lactoferrin, and calprotectin were determined by enzyme-linked immunosorbent assay (ELISA) and compared in different groups. Other clinical symptoms or signs, including fever, vomiting, diarrhea, abdominal pain, bloated abdomen, daily intake, appetite, and body weight were also assessed. Results: Data were collected from 81 individuals across three different time points. Total fecal IgA levels in fecal extracts of the probiotics group were higher than those in the control group, reaching statistical significance (p < 0.05). Concentrations of fecal lactoferrin and calprotectin were significantly downregulated in patients with probiotic Lactobacillus casei variety rhamnosus (Lc) consumption compared to those of the control (p < 0.05). Probiotic Lc administration may be beneficial for gut-microbiota modulation, as shown by the data collected at one week after enrollment. Counts of Bifidobacteria and Lactobacillus species were elevated in stool culture of the probiotic group. Appetite and oral intake, body-weight gain, abdominal pain, bloating, as well as bowel habits (diarrhea) were much better in children receiving probiotics compared with those in the control group. Conclusion: Fecal IgA increased during acute diarrhea under Lc treatment; in contrast, fecal lactoferrin and calprotectin were downregulated during acute diarrhea under Lc treatment. Probiotic Lc may be a useful supplement for application in children during acute diarrhea to reduce clinical severity and intestinal inflammatory reaction.
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He, Yuke, Kevin Mok, Pramote Chumnanpuen, Massalin Nakphaichit und Wanwipa Vongsangnak. „Dissecting Metabolic Functions and Sugar Transporters Using Genome and Transportome of Probiotic Limosilactobacillus fermentum KUB-D18“. Genes 16, Nr. 3 (17.03.2025): 348. https://doi.org/10.3390/genes16030348.
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Background/Objectives: Limosilactobacillus fermentum KUB-D18, a heterofermentative lactic acid bacterium with promising probiotic properties, is known for promoting gut health and nutrient absorption. Originally isolated from chicken intestines, this strain demonstrates versatile metabolic capabilities in diverse gastrointestinal environments. However, the metabolic functions and sugar transport-related genes remain largely unexplored. This study thus aimed to dissect metabolic functions and sugar transports of L. fermentum KUB-D18. Methods: Next-generation and third-generation sequencing techniques using integrative genomic platform towards transportome analysis were performed. Results: The complete genome, sized at 2.12 Mbps with a GC content of 51.36%, revealed 2079 protein-encoding genes, of which 1876 protein functions were annotated and identified in top categories involved in amino acids, nucleotide, energy, and carbohydrate transports and metabolisms. Comparative genes analysis identified 50 core and 12 strain-specific genes linked to probiotic properties, e.g., acid resistances and bile tolerances, antioxidant functions, or anti-inflammatory properties. Further, sugar transportome analysis uncovered 57 transporter genes, demonstrating diverse carbon utilization and phosphotransferase (PTS) systems, corroborated by API 50 CHL test results for carbohydrate metabolism profile. Conclusions: These findings enhance the comprehensive metabolic understanding of L. fermentum KUB-D18, supporting its industrial potential and applications in engineered probiotics.
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Boutin, Sébastien, Céline Audet und Nicolas Derome. „Probiotic treatment by indigenous bacteria decreases mortality without disturbing the natural microbiota of Salvelinus fontinalis“. Canadian Journal of Microbiology 59, Nr. 10 (Oktober 2013): 662–70. http://dx.doi.org/10.1139/cjm-2013-0443.
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Next-generation sequencing is revealing the complex interactive networks of host–bacteria interactions, as it is now possible to screen in detail the microbiota harbored by a host. This study investigated the influence of a probiotic treatment on the survival and microbiota of brook charr (Salvelinus fontinalis), focusing on its disturbance of the natural microbiota (dysbiosis). The results indicated that an indigenous probiotic strain (identified as Rhodococcus sp.) colonized neither the fish skin mucus nor the water following the probiotic treatment. Instead, the probiotic strain was detected only in the biofilm of the test tank. Nevertheless, a substantial beneficial effect of the probiotic treatment was observed: the population of the pathogen Flavobacterium psychrophilum decreased in the treated tank water. This study clearly shows that the indigenous strain chosen for the probiotic treatment did not disturb the natural fish skin mucus microbiota but acted directly through the production system to control the growth of the pathogen and, as a consequence, to enhance fish survival.
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He, Siqin, Liqiong Song, Yuchun Xiao, Yuanming Huang und Zhihong Ren. „Genomic, Probiotic, and Functional Properties of Bacteroides dorei RX2020 Isolated from Gut Microbiota“. Nutrients 17, Nr. 6 (18.03.2025): 1066. https://doi.org/10.3390/nu17061066.
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Background/Objectives: Gut microbiota is essential for maintaining host immune homeostasis and has been confirmed to be closely related to some intestinal and extraintestinal diseases. Bacteroides, as the dominant bacterial genus in the human gut, has attracted great attention due to its excellent metabolic activity, but there are few studies on Bacteroides dorei species. In our previous study, a gut commensal strain, Bacteroides dorei RX2020 (B. dorei), was isolated from healthy human feces and exhibited superior flavonoid metabolic activity, prompting further analysis of its uncharacterized genomic features, probiotic potential, safety, and immunomodulatory activity. Results: The results showed that B. dorei exhibited intrinsic probiotic functionalities with preserved genomic and phenotypic stability, demonstrated safety profiles in murine models through in vivo assessments, and conferred antagonistic activity against enteric foodborne pathogens via competitive exclusion. The strain also demonstrated abundant metabolic activity and was involved in the metabolism of tryptophan and bile acids (BAs). Moreover, B. dorei can promote the production of IFNβ by dendritic cells (DCs) to inhibit the replication of influenza virus in epithelial cells, which may be achieved by regulating host metabolism. Conclusions: This study reveals the potential of B. dorei as next-generation probiotics (NGPs), contributing to a broader understanding and application of these novel probiotics in health and disease management.
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Tochio, Takumi, Yoshihiro Kadota, Toshio Tanaka und Yasuhiro Koga. „1-Kestose, the Smallest Fructooligosaccharide Component, Which Efficiently Stimulates Faecalibacterium prausnitzii as Well as Bifidobacteria in Humans“. Foods 7, Nr. 9 (01.09.2018): 140. http://dx.doi.org/10.3390/foods7090140.
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The concept of prebiotics was established more than 30 years ago. While the prebiotic concept has now expanded thus includes non-carbohydrate substances and diverse categories other than foods, fructooligosaccharides (FOS) have still predominantly been used as pebiotics, because the effects of FOS exclusively act through the enrichment of Bifidobacterium and Lactobacillus spp., which have been classified as beneficial intestinal commensals so far. Now the commercially available FOS products are synthetic mixture of several kinds of FOS components including 1-kestose (GF2), nystose (GF3) and GF4. In our previous studies, superiority of 1-kestose to the longer-chain FOS components such as nystose with regard to bifidogenic activity was clearly demonstrated. Recently, a broader range of beneficial bacteria including butyrate-producing indigenous bacteria have been recognized and expected to be new probiotic strains. Among them, resident Faecalibacterium prausnitzii is a butyrate producer with a significant anti-inflammatory effect thus expected to be useful as a next-generation probiotic. However, this bacterium is extremely oxygen-sensitive thus can be difficult to grow industrially. On the other hand, we have clearly demonstrated a significant prebiotic effect of 1-kestose, which is the smallest component of FOS, on F. prausnitzii in the gut of humans. These findings suggest that 1-kestose has impressive potential as a new prebiotic targeting F. prausnitzii, a next-generation probiotic strain, as well as bifidobacteria.
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Fontana, Federico, Giulia Alessandri, Gabriele Andrea Lugli, Leonardo Mancabelli, Giulia Longhi, Rosaria Anzalone, Alice Viappiani, Marco Ventura, Francesca Turroni und Christian Milani. „Probiogenomics Analysis of 97 Lactobacillus crispatus Strains as a Tool for the Identification of Promising Next-Generation Probiotics“. Microorganisms 9, Nr. 1 (30.12.2020): 73. http://dx.doi.org/10.3390/microorganisms9010073.
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Members of the genus Lactobacillus represent the most common colonizers of the human vagina and are well-known for preserving vaginal health and contrasting the colonization of opportunistic pathogens. Remarkably, high abundance of Lactobacillus crispatus in the vaginal environment has been linked to vaginal health, leading to the widespread use of many L. crispatus strains as probiotics. Nevertheless, despite the scientific and industrial relevance of this species, a comprehensive investigation of the genomics of L. crispatus taxon is still missing. For this reason, we have performed a comparative genomics analysis of 97 L. crispatus strains, encompassing 16 strains sequenced in the framework of this study alongside 81 additional publicly available genome sequences. Thus, allowing the dissection of the L.crispatus pan-genome and core-genome followed by a comprehensive phylogenetic analysis based on the predicted core genes that revealed clustering based on ecological origin. Subsequently, a genomics-targeted approach, i.e., probiogenomics analysis, was applied for in-depth analysis of the eight L. crispatus strains of human origin sequenced in this study. In detail their genetic repertoire was screened for strain-specific genes responsible for phenotypic features that may guide the identification of optimal candidates for next-generation probiotics. The latter includes bacteriocin production, carbohydrates transport and metabolism, as well as a range of features that may be responsible for improved ecological fitness. In silico results regarding the genetic repertoire involved in carbohydrate metabolism were also validated by growth assays on a range of sugars, leading to the selection of putative novel probiotic strains.
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Vernay, T., I. Cannie, F. Gaboriau, S. David-Le Gall, Z. Tamanai-Shacoori, A. Burel, A. Jolivet-Gougeon, O. Loréal und L. Bousarghin. „Bacteroides fragilis prevents Salmonella Heidelberg translocation in co-culture model mimicking intestinal epithelium“. Beneficial Microbes 11, Nr. 4 (12.08.2020): 391–401. http://dx.doi.org/10.3920/bm2020.0004.
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Salmonella Heidelberg is one of the most common serovar causing foodborne illnesses. To limit the development of digestive bacterial infection, food supplements containing probiotic bacteria can be proposed. Commensal non-toxigenic Bacteroides fragilis has recently been suggested as a next-generation probiotic candidate. By using an original triple co-culture model including Caco-2 cells (representing human enterocytes), HT29-MTX (representing mucus-secreting goblet cells), and M cells differentiated from Caco-2 by addition of Raji B lymphocytes, bacterial translocation was evaluated. The data showed that S. Heidelberg could translocate in the triple co-culture model with high efficiency, whereas for B. fragilis a weak translocation was obtained. When cells were exposed to both bacteria, S. Heidelberg translocation was inhibited. The cell-free supernatant of B. fragilis also inhibited S. Heidelberg translocation without impacting epithelial barrier integrity. This supernatant did not affect the growth of S. Heidelberg. The non-toxigenic B. fragilis confers health benefits to the host by reducting bacterial translocation. These results suggested that the multicellular model provides an efficient in vitro model to evaluate the translocation of pathogens and to screen for probiotics that have a potential inhibitory effect on this translocation.
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Dai, Wei, Youjing Lv, Min Quan, Mingfeng Ma, Qingsen Shang und Guangli Yu. „Bacteroides salyersiae Is a Candidate Probiotic Species with Potential Anti-Colitis Properties in the Human Colon: First Evidence from an In Vivo Mouse Model“. Nutrients 16, Nr. 17 (01.09.2024): 2918. http://dx.doi.org/10.3390/nu16172918.
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Previous studies have indicated a critical role of intestinal bacteria in the pathogenesis of ulcerative colitis (UC). B. salyersiae is a commensal species from the human gut microbiota. However, what effect it has on UC development has not been investigated. In the present study, we explored this issue and demonstrated for the first time that oral administration of B. salyersiae CSP6, a bacterium previously isolated from the fecal sample of a healthy individual, protected against dextran sulfate sodium (DSS)-induced colitis in C57BL/6J mice. In particular, B. salyersiae CSP6 improved mucosal damage and attenuated gut dysbiosis in the colon of DSS-fed mice. Specifically, B. salyersiae CSP6 decreased the population of pathogenic Escherichia-Shigella spp. and increased the abundance of probiotic Dubosiella spp. and Bifidobacterium pseudolongum. Additionally, by reshaping the colonic microbiota, B. salyersiae CSP6 remarkably increased the fecal concentrations of equol, 8-deoxylactucin, and tiglic acid, three beneficial metabolites that have been well documented to exert strong anti-inflammatory effects. Altogether, our study provides novel evidence that B. salyersiae is a candidate probiotic species with potential anti-colitis properties in the human colon, which has applications for the development of next-generation probiotics.
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Amat, Samat, Hannah Lantz, Peris M. Munyaka und Benjamin P. Willing. „Prevotella in Pigs: The Positive and Negative Associations with Production and Health“. Microorganisms 8, Nr. 10 (14.10.2020): 1584. http://dx.doi.org/10.3390/microorganisms8101584.
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A diverse and dynamic microbial community (known as microbiota) resides within the pig gastrointestinal tract (GIT). The microbiota contributes to host health and performance by mediating nutrient metabolism, stimulating the immune system, and providing colonization resistance against pathogens. Manipulation of gut microbiota to enhance growth performance and disease resilience in pigs has recently become an active area of research in an era defined by increasing scrutiny of antimicrobial use in swine production. In order to develop microbiota-targeted strategies, or to identify potential next-generation probiotic strains originating from the endogenous members of GIT microbiota in pigs, it is necessary to understand the role of key commensal members in host health. Many, though not all, correlative studies have associated members of the genus Prevotella with positive outcomes in pig production, including growth performance and immune response; therefore, a comprehensive review of the genus in the context of pig production is needed. In the present review, we summarize the current state of knowledge about the genus Prevotella in the intestinal microbial community of pigs, including relevant information from other animal species that provide mechanistic insights, and identify gaps in knowledge that must be addressed before development of Prevotella species as next-generation probiotics can be supported.
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Yue, Mengyun, Jing Wei, Wenjie Chen, Daojun Hong, Tingtao Chen und Xin Fang. „Neurotrophic Role of the Next-Generation Probiotic Strain L. lactis MG1363-pMG36e-GLP-1 on Parkinson’s Disease via Inhibiting Ferroptosis“. Nutrients 14, Nr. 22 (18.11.2022): 4886. http://dx.doi.org/10.3390/nu14224886.
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Parkinson’s disease (PD) is a neurodegenerative disease (NDD) with high and ongoing morbidity, bringing heavy burdens to PD patients seriously. Finding neurotrophic drugs still remains vital due to the limited drug spectrum available currently. Substantial evidence suggests that glucagon-like peptide 1 (GLP-1) exerts neuroprotection on PD, yet the short-lived biological activity markedly hindered its application. Herein, we investigated the neurotrophic role of the next-generation probiotic strain L. lactis MG1363-pMG36e-GLP-1 in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD mice and elucidated the mechanisms. Our data suggested that L. lactis MG1363-pMG36e-GLP-1 markedly enhanced motor deficits induced by MPTP via rescuing dopaminergic (DAergic) neurodegeneration in substantia nigra (SN). We found that L. lactis MG1363-pMG36e-GLP-1 exerts neurotrophic effects via activating the Keap1/Nrf2/GPX4 signalling pathway to down-regulate ACSL4 and up-regulate FSP1 to suppress ferroptosis. Additionally, the decreased oxidative stress levels via suppressing generations of ROS and MDA supported our findings. Lastly, we identified that the L. lactis MG1363-pMG36e-GLP-1 administration reversed dysbiosis in PD mice by increasing Akkermansia, Oscillospira, and Sutterella at the genus level. These results indicated that the neurotrophic effects of the next-generation probiotics L. lactis MG1363-pMG36e-GLP-1 against MPTP-induced Parkinsonism are mediated by modulating oxidative stress, inhibiting ferroptosis, and redressing dysbiosis.
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Abadi, Agung Setia, Anik Hartinah Mariati und Ating Yuniarti. „PROBIOTIK INDIGENOUS Bacillus sp (NL004) YANG DIPERKAYA PADA LIMBAH CAIR TAHU (LCT) TERHADAP PERTUMBUHAN IKAN NILA (Oreochromis niloticus)“. Media Akuakultur 18, Nr. 1 (29.09.2023): 15. http://dx.doi.org/10.15578/ma.18.1.2023.15-20.
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Telah diketahui bahwa penambahan probiotik dipercaya meningkatkan keuntungan dengan cara meningkatkan pertumbuhan dan resistensi serangan penyakit pada ikan. Tujuan penelitian ini adalah untuk mengetahui efektivitas penambahan probiotik indigenous pada laju pertumbuhan spesifik ikan nila (Oreocromis niloticus). Metode yang digunakan dalam penelitian ini dilakukan dengan cara mengisolasi, karakterisasi, dan identifikasi bakteri indigenous dari usus ikan nila. Selanjutnya dilakukan kultur bakteri kandidat probiotik pada limbah cair tahu, dan dilakukan uji viabilitas pada pakan. Tahap selanjutnya dilakukan uji in-vivo dengan menggunakan rancangan acak lengkap yang diulang sebanyak dua kali. Perlakuan A tanpa penambahan probiotik, B dengan penambahan probiotik NL 004 107 cfu/ml/gr. Parameter uji yang dilakukan diantaranya sintasan, laju pertumbuhan spesifik, konversi pakan dan efisiensi pakan. Hasil uji menunjukkan bahwa penambahan C:N rasio pada LCT mempunyai waktu generasi sel lebih baik sebesar 0,29 (generasi/menit), viabilitas pada pakan menunjukkan pertumbuhan sel lebih baik pada dosis 107 cfu/ml/gr. Hasil uji karakter pertumbuhan ikan menunjukkan penambahan probiotik NL 004 meningkatkan laju pertumbuhan spesifik sebesar 2,44 % bb/hari, FCR menurun sebesar 1,09 dan meningkatkan efisiensi pakan sebesar 75,79%.It is known that the addition of probiotics is believed to increase profits by increasing growth and disease resistance in fish. The purpose of this study was to determine the effectiveness of adding indigenous probiotics to the specific growth rate of tilapia (Oreocromis niloticus). The method used in this research is by isolating, characterizing, and identifying bacteria from the intestines of tilapia. Furthermore, probiotic candidate bacteria culture was carried out in tofu liquid waste (TLW), and viability tests were carried out on the feed. The next stage is an in-vivo test using a completely randomized design which was two repetitions. Treatment A without the addition of probiotics, B with the addition of probiotics NL 004 107 cfu.ml-1.gr-1. The test parameters carried out include survival, specific growth rate, feed conversion, and feed efficiency. The test results showed that the addition of C:N ratio in TLW had a better cell generation time of 0.29 (generation.min-1), and viability on feed showed better cell growth at a dose of 107 cfu.ml-1.gr-1. The result of the fish growth character test showed that the addition of probiotics increased the specific growth rate by 2.44% w.day-1, decreased FCR by 1.09, and increased feed efficiency by 75.79%.
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Grondin, Jensine, Huaqing Wang, Sabah Haq, Yun Han Kwon, Lesley MacNeil, Michael Surette und Waliul Khan. „Tu1887 EFFECTS OF THE NEXT GENERATION PROBIOTIC, AKKERMANSIA MUCINIPHILA, ON INTESTINAL INFLAMMATION AND BARRIER FUNCTION“. Gastroenterology 164, Nr. 6 (Mai 2023): S—1152—S—1153. http://dx.doi.org/10.1016/s0016-5085(23)03655-7.
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Grondin, J., H. Wang, S. Haq, Y. H. Kwon, L. MacNeil, M. Surette und W. Khan. „A33 EFFECTS OF THE NEXT GENERATION PROBIOTIC, AKKERMANSIA MUCINIPHILA, ON INTESTINAL INFLAMMATION AND BARRIER FUNCTION“. Journal of the Canadian Association of Gastroenterology 6, Supplement_1 (01.03.2023): 18. http://dx.doi.org/10.1093/jcag/gwac036.033.
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Abstract Background Inflammatory bowel disease (IBD), characterised by chronic intestinal inflammation, is hypothesised to arise from the interplay between susceptibility genes, the immune system, environmental factors, and gut microbiota. Akkermansia muciniphila is a symbiotic bacterium that accounts for 1-5% of the human fecal microbiota. This microbe has been hailed as a next-generation probiotic, principally with regard to its plethora of beneficial host interactions, including the ability to influence mucin secretion and strengthen the intestinal barrier. Purpose Though a clear-cut role and mechanism by which A. muciniphila influences inflammatory conditions is unknown, evidence indicates this microbe is depleted in IBD, suggesting it may have protective effects that are lost in these conditions. Here, we investigate the role and mechanism of A. muciniphila in intestinal inflammation and its influence on intestinal barrier function by utilizing barrier-disrupting models of colitis. Method Across several experimental models of intestinal inflammation including the chemically-induced dextran sulphate sodium (DSS) model, the parasitic-based model of Trichuris muris infection, and the spontaneous Muc2-/- model, A.muciniphila was administered by oral gavage. Disease activity index, macroscopic scoring and histological scoring were all performed to assess the severity of intestinal inflammation. Various pro- and anti-inflammatory cytokines were assessed within colonic tissue using commercially available ELISA kits.To investigate the effects that A. muciniphila has on barrier function in the context of colitis, reverse transcriptase qPCR was used to explore several factors, including several TJPs, AMPs, and mucins. To analyse the composition of the microbiota and changes in diversity with A. muciniphila supplementation, 16S rRNA sequencing of fecal samples was performed. Result(s) Though only minor benefits were derived from this microbe in germ-free mice, in specific pathogen-free (SPF) mice, administration of pasteurized A. muciniphila in a DSS recovery model ameliorated inflammation severity and promoted recovery compared to controls. When gavaged prior to DSS administration, both live and pasteurized A. muciniphila failed to diminish inflammatory markers indicating minimal preventative effects. T. muris-infected SPF mice treated with live A. muciniphila showed increased levels of Th2 and anti-inflammatory cytokines, decreased worm burden, and enhanced levels of the mucin, Muc5ac, compared with those receiving control broth or pasteurized bacteria. Further, both live and pasteurized A. muciniphila ameliorated the severity of inflammation in a mucin 2 deficient (Muc2-/-) mouse model of spontaneous colitis, indicating that these protective effects are Muc2-independent. Conclusion(s) These observations provide us not only with an enhanced understanding of the role A. muciniphila plays in the pathogenesis of intestinal inflammatory conditions but also may fuel novel avenues of treatment for those with IBD. Please acknowledge all funding agencies by checking the applicable boxes below CIHR Disclosure of Interest None Declared
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Gryaznova, Mariya, Inna Burakova, Yuliya Smirnova, Polina Morozova, Egor Chirkin, Artem Gureev, Evgeny Mikhaylov, Olga Korneeva und Mikhail Syromyatnikov. „Effect of Probiotic Bacteria on the Gut Microbiome of Mice with Lipopolysaccharide-Induced Inflammation“. Microorganisms 12, Nr. 7 (30.06.2024): 1341. http://dx.doi.org/10.3390/microorganisms12071341.
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The role of lipopolysaccharide (LPS) in the development of diseases is clear, but the specific mechanisms remain poorly understood. This study aimed to investigate the microbiome aberrations in the guts of mice against the background of LPS, as well as the anti-inflammatory effect of probiotic supplementation with Lactobacillus plantarum from the gut, a mix of commercial probiotic lactic acid bacteria, and Weissella confusa isolated from milk using next-generation sequencing. LPS injections were found to induce inflammatory changes in the intestinal mucosa. These morphological changes were accompanied by a shift in the microbiota. We found no significant changes in the microbiome with probiotic supplementation compared to the LPS group. However, when Lactobacillus plantarum and a mix of commercial probiotic lactic acid bacteria were used, the intestinal mucosa was restored. Weissella confusa did not contribute to the morphological changes of the intestinal wall or the microbiome. Changes in the microbiome were observed with probiotic supplementation of Lactobacillus plantarum and a mix of commercial probiotic lactic acid bacteria compared to the control group. In addition, when Lactobacillus plantarum was used, we observed a decrease in the enrichment of the homocysteine and cysteine interconversion pathways with an increase in the L-histidine degradation pathway.
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Gilfillan, Darby, Allison C. Vilander, Meichen Pan, Yong Jun Goh, Sarah O’Flaherty, Ningguo Feng, Bridget E. Fox et al. „Lactobacillus acidophilus Expressing Murine Rotavirus VP8 and Mucosal Adjuvants Induce Virus-Specific Immune Responses“. Vaccines 11, Nr. 12 (28.11.2023): 1774. http://dx.doi.org/10.3390/vaccines11121774.
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Rotavirus diarrhea-associated illness remains a major cause of global death in children under five, attributable in part to discrepancies in vaccine performance between high- and low-middle-income countries. Next-generation probiotic vaccines could help bridge this efficacy gap. We developed a novel recombinant Lactobacillus acidophilus (rLA) vaccine expressing rotavirus antigens of the VP8* domain from the rotavirus EDIM VP4 capsid protein along with the adjuvants FimH and FliC. The upp-based counterselective gene-replacement system was used to chromosomally integrate FimH, VP8Pep (10 amino acid epitope), and VP8-1 (206 amino acid protein) into the L. acidophilus genome, with FliC expressed from a plasmid. VP8 antigen and adjuvant expression were confirmed by flow cytometry and Western blot. Rotavirus naïve adult BALB/cJ mice were orally immunized followed by murine rotavirus strain ECWT viral challenge. Antirotavirus serum IgG and antigen-specific antibody-secreting cell responses were detected in rLA-vaccinated mice. A day after the oral rotavirus challenge, fecal antigen shedding was significantly decreased in the rLA group. These results indicate that novel rLA constructs expressing VP8 can be successfully constructed and used to generate modest homotypic protection from rotavirus challenge in an adult murine model, indicating the potential for a probiotic next-generation vaccine construct against human rotavirus.
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Szydłowska, Aleksandra, Dorota Zielińska, Barbara Sionek und Danuta Kołożyn-Krajewska. „Factors Affecting the Quality of Probiotic Plant-Based Frozen Desserts—The Authors’ Own Experiments in the Context of the Literature“. Fermentation 10, Nr. 6 (30.05.2024): 291. http://dx.doi.org/10.3390/fermentation10060291.
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Recently, there has been worldwide growth in consumer nutrition awareness, which has resulted in a market-driven increase in the demand for “functional food”, which, in addition to traditional nutrients, also contains ingredients with specific properties that have a beneficial effect on human health. One of the types of functional food is so-called “probiotic food”, which includes, for example, frozen desserts. These products appear attractive to the consumer because of their sensory, nutritional and refreshing qualities. Due to progress in science, genetics, the acquisition of new sources of probiotic microorganisms and new plant varieties, the beneficial effects of the characteristic metabolites of the microbiome—so-called postbiotics—and also aspects of NGPs (Next Generation Probiotics), work is under way to optimize the technology used during the production of such products. At the same time, there is an observed market-based increase in the supply of new formulations based only on plant-origin materials with different technological modifications, including prebiotic enrichment, which allows for the production of a synbiotic product. Therefore, the objective of this study is a narrative review, in combination with the authors’ own experiments, concerning the impact of various factors on functional, plant-origin frozen desserts, from the point of view of maintaining their quality.