Thematische Bibliographien / NHPC

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Auswahl der wissenschaftlichen Literatur zum Thema „NHPC“

Autor: Grafiati
Veröffentlicht am 11. September 2023

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Zeitschriftenartikel zum Thema "NHPC"

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Sakashita, Toshihide, Yoshiteru Hashimoto, Ken-Ichi Oinuma und Michihiko Kobayashi. „Transcriptional Regulation of the Nitrile Hydratase Gene Cluster in Pseudomonas chlororaphis B23“. Journal of Bacteriology 190, Nr. 12 (11.04.2008): 4210–17. http://dx.doi.org/10.1128/jb.00061-08.

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ABSTRACT An enormous amount of nitrile hydratase (NHase) is inducibly produced by Pseudomonas chlororaphis B23 after addition of methacrylamide as the sole nitrogen source to a medium. The expression pattern of the P. chlororaphis B23 NHase gene cluster in response to addition of methacrylamide to the medium was investigated. Recently, we reported that the NHase gene cluster comprises seven genes (oxdA, amiA, nhpA, nhpB, nhpC, nhpS, and acsA). Sequence analysis of the 1.5-kb region upstream of the oxdA gene revealed the presence of a 936-bp open reading frame (designated nhpR), which should encode a protein with a molecular mass of 35,098. The deduced amino acid sequence of the nhpR product showed similarity to the sequences of transcriptional regulators belonging to the XylS/AraC family. Although the transcription of the eight genes (nhpR, oxdA, amiA, nhpABC, nhpS, and acsA) in the NHase gene cluster was induced significantly in the P. chlororaphis B23 wild-type strain after addition of methacrylamide to the medium, transcription of these genes in the nhpR disruptant was not induced, demonstrating that nhpR codes for a positive transcriptional regulator in the NHase gene cluster. A reverse transcription-PCR experiment revealed that five genes (oxdA, amiA, nhpA, nhpB, and nhpC) are cotranscribed, as are two other genes (nhpS and acsA). The transcription start sites for nhpR, oxdA, nhpA, and nhpS were mapped by primer extension analysis, and putative −12 and −24 σ54-type promoter binding sites were identified. NhpR was found to be the first transcriptional regulator of NHase belonging to the XylS/AraC family.
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Zou, Zhanghua, Yu Lei, Yingming Li, Yanhua Zhang und Wei Xiao. „Nitrogen-Doped Hierarchical Meso/Microporous Carbon from Bamboo Fungus for Symmetric Supercapacitor Applications“. Molecules 24, Nr. 20 (12.10.2019): 3677. http://dx.doi.org/10.3390/molecules24203677.

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We report the synthesis of nitrogen-doped hierarchical meso/microporous carbon using renewable biomass bamboo fungus as precursor via two-step pyrolysis processes. It is found that the developed porous carbon (NHPC-800) features honeycomb-like cellular framework with well-developed porosity, huge specific surface area (1708 m2 g−1), appropriate nitrogen-doping level (3.2 at.%) and high mesopore percentage (25.5%), which are responsible for its remarkable supercapacitive performances. Electrochemical tests suggest that the NHPC-800 electrode offers the largest specific capacitance of 228 F g−1, asplendid rate capability and stable electrochemical behaviors in a traditional three-electrode system. Additionally, asymmetric supercapacitor device is built based on this product as well. An individual as-assembled supercapacitor of NHPC-800//NHPC-800 delivers the maximum energy density of 4.3 Wh kg−1; retains the majority of capacitanceat large current densities; and shows terrific cycling durability with negligible capacitance drop after long-term charge/discharge for beyond 10,000 cycles even at a high current density of 10 A g−1. These excellent supercapacitive properties of NHPC-800 in both three- and two-electrode setups outperform those of lots of biomass-derived porous carbons and thus make it a perspective candidate for producing cost-effective and high-performance supercapacitors
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Ba, Yuerong, Wei Pan, Shangchao Pi, Yaomin Zhao und Liwei Mi. „Nitrogen-doped hierarchical porous carbon derived from a chitosan/polyethylene glycol blend for high performance supercapacitors“. RSC Advances 8, Nr. 13 (2018): 7072–79. http://dx.doi.org/10.1039/c8ra00016f.

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Wu, X. X., und H. Zhou. „Hierarchical porous N-doped carbon supported palladium (Pd/NHPC) as a sustainable catalyst for the reduction of 4-nitrophenol with good activity and lifetime“. New Journal of Chemistry 41, Nr. 18 (2017): 10245–50. http://dx.doi.org/10.1039/c7nj01947e.

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Tang, Minghui, Jiang Deng, Mingming Li, Xuefeng Li, Haoran Li, Zhirong Chen und Yong Wang. „3D-interconnected hierarchical porous N-doped carbon supported ruthenium nanoparticles as an efficient catalyst for toluene and quinoline hydrogenation“. Green Chemistry 18, Nr. 22 (2016): 6082–90. http://dx.doi.org/10.1039/c6gc01858k.

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Wang, Wenxiu, Hongying Quan, Weimin Gao, Ren Zou, Dezhi Chen, Yinghu Dong und Lin Guo. „N-Doped hierarchical porous carbon from waste boat-fruited sterculia seed for high performance supercapacitors“. RSC Advances 7, Nr. 27 (2017): 16678–87. http://dx.doi.org/10.1039/c7ra01043e.

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Cao, Guiqiang, Da Bi, Jingxiang Zhao, Jing Zheng, Zhikang Wang, Qingxue Lai und Yanyu Liang. „Transformation of ZIF-8 nanoparticles into 3D nitrogen-doped hierarchically porous carbon for Li–S batteries“. RSC Advances 10, Nr. 29 (2020): 17345–52. http://dx.doi.org/10.1039/c9ra10063f.

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Li, Min, Feng Liu, Supeng Pei, Zongshang Zhou, Kai Niu, Jianbo Wu und Yongming Zhang. „Synthesis of Platinum Nanocrystals Dispersed on Nitrogen-Doped Hierarchically Porous Carbon with Enhanced Oxygen Reduction Reaction Activity and Durability“. Nanomaterials 13, Nr. 3 (21.01.2023): 444. http://dx.doi.org/10.3390/nano13030444.

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Platinum-based catalysts are widely used for efficient catalysis of the acidic oxygen reduction reaction (ORR). However, the agglomeration and leaching of metallic Pt nanoparticles limit the catalytic activity and durability of the catalysts and restrict their large-scale commercialization. Therefore, this study aimed to achieve a uniform distribution and strong anchoring of Pt nanoparticles on a carbon support and improve the ORR activity and durability of proton-exchange membrane fuel cells. Herein, we report on the facile one-pot synthesis of a novel ORR catalyst using metal–nitrogen–carbon (M–N–C) bonding, which is formed in situ during the ion exchange and pyrolysis processes. An ion-exchange resin was used as the carbon source containing R-N+(CH3)3 groups, which coordinate with PtCl62− to form nanosized Pt clusters confined within the macroporous framework. After pyrolysis, strong M-N-C bonds were formed, thereby preventing the leaching and aggregation of Pt nanoparticles. The as-synthesized Pt supported on the N-doped hierarchically porous carbon catalyst (Pt/NHPC-800) showed high specific activity (0.3 mA cm−2) and mass activity (0.165 A mgPt−1), which are approximately 2.7 and 1.5 times higher than those of commercial Pt/C, respectively. The electrochemical surface area of Pt/NHPC-800 remained unchanged (~1% loss) after an accelerated durability test of 10,000 cycles. The mass activity loss after ADT of Pt/NHPC-800 was 18%, which is considerably lower than that of commercial Pt/C (43%). Thus, a novel ORR catalyst with highly accessible and homogeneously dispersed Pt-N-C sites, high activity, and durability was successfully prepared via one-pot synthesis. This facile and scalable synthesis strategy for high-efficiency catalysts guides the further synthesis of commercially available ORR catalysts.
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Dahal, Milan. „Sikkim’s Initiatives in Hydropower“. Hydro Nepal: Journal of Water, Energy and Environment 3 (26.05.2009): 38–41. http://dx.doi.org/10.3126/hn.v3i0.1919.

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Sikkim is rich in hydropower potential in spite of its small area. National Hydroelectric Power Corporation Limited (NHPC) and other private developers are entering in hydropower sector of Sikkim. Though some of local people are in the protest of dams, the Government of Sikkim is hopeful and determines to achieve benefit largely from hydropower Keywords: Sikkim, Himalayas, hydropower, environment doi: 10.3126/hn.v3i0.1919 Hydro Nepal Journal of Water, Energy and Environment Issue No. 3, July 2008. Page: 38-41
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Patel, Asish, Sukhwinder Kaur, Lynette Smith, Chandrakanth Are und Surinder Batra. „Diagnostic potential of mucins in pancreatic juice for pancreatic cancer.“ Journal of Clinical Oncology 34, Nr. 4_suppl (01.02.2016): 222. http://dx.doi.org/10.1200/jco.2016.34.4_suppl.222.

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222 Background: Pancreatic juice remains an underutilized resource for diagnosing pancreatic cancer. Mucins are high molecular weight glycoproteins differentially upregulated in pancreatic cancer, and we hypothesize that their profile in pancreatic juice may have diagnostic potential. Methods: Pancreatic juice was obtained during endoscopy from non-healthy non-pancreatic control (NHPC, n = 57), chronic pancreatitis (CP, n = 23), and pancreatic cancer (PC, n = 23) patients. Sandwich ELISA was used to detect MUC1, MUC4, MUC5AC, CA125, and CA19-9. Kruskal-Wallis test and Wilcoxon rank sum test for group and pairwise comparison was done with p < 0.05 as significant. Logistic regression with ROC curve modeling of log transformed data was done for each biomarker individually and in combination to determine odds ratio (OR), sensitivity (SN), and specificity (SP) for PC. Results: PC vs NHPC: MUC5AC had the best individual performance for diagnosing PC with an OR = 2.78 (95% CI = 1.51-5.13), AUC = 0.81, and optimal SN/SP of 0.83 and 0.67, respectively. CA125 was increased in PC with an OR = 2.31 (95% CI = 1.4-4.0), AUC = 0.73, and optimal SN/SP of 0.88 and 0.67. CA19-9 was increased in PC with an OR = 1.5 (95% CI = 1.2-1.8), AUC = 0.76, and optimal SN/SP of 0.73 and 0.70. A combination of MUC1, MUC5AC, CA125, and CA19-9 outperformed all individual markers and had the largest AUC (0.89) with optimal SN/SP of 0.84 and 0.79. PC vs CP: MUC1 concentration in PC was significantly less than CP with an OR = 0.21 (95%CI = 0.088-0.49), AUC = 0.82, and optimal SN/SP of 0.87 and 0.78. PC vs NHPC+CP: MUC1 was decreased significantly in PC with an OR = 0.65 (95% CI = 0.44-0.96), AUC = 0.69, and optimal SN/SP of 0.87 and 0.63. CA125 was increased in PC with an OR = 1.64 (95%CI = 1.1-2.4), AUC = 0.66, and optimal SN/SP of 0.67 and 0.64. CA19-9 was increased in PC with an OR = 1.32 (95%CI = 1.1-1.6), AUC = 0.68, and optimal SN/SP of 0.63 and 0.67. A combination of MUC1, MUC5AC, CA125, and CA19-9 had an AUC = 0.86 with optimal SN/SP of 0.87 and 0.77 for PC. Conclusions: MUC1, MUC5AC, CA125, and CA19-9 combination provides a significantly improved diagnostic panel compared to any individual marker in pancreatic juice for detecting malignancy.
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Dissertationen zum Thema "NHPC"

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RASHID, FAAKIRAH. „STABILITY ANALYSIS OF HIMALAYAN SLOPES“. Thesis, DELHI TECHNOLOGICAL UNIVERSITY, 2021. http://dspace.dtu.ac.in:8080/jspui/handle/repository/18785.

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There is a large number of destabilized slopes in the Himalayan region mainly due to geological environment which is complex and having active tectonics present in the area. There is also deformation and metamorphism in the varied lithology of area which results in a rugged topography. The instability in the slopes is also being accelerated by the activities resulting from various ongoing construction and tunneling processes for the development in the region. There has been huge economic losses and a serious number of mishaps, which is endangering the Himalayan ecosystem due to various landslide related problems. The Himalayan region experiences frequent landslides along the national highways and mountain roads particularly the sections where there is debris slope. Factor of safety for each of the slopes under construction has been determined using numerical analysis first and then GEOSTUDIO correspondingly. In order to increase the stability of slope in the most efficient stabilization and ground improvement techniques have been proposed. It is very important that in the various vulnerable sections of the Himalayas slope stability assessment should be carried out. The site under consideration is Razdan Pass which is a high mountain pass at 3557m above the sea level. It is located in the Gurez valley of Jammu and Kashmir which is the highest point which connects Bandipora with Gurez. The road over the pass is winding and is called Bandipora-Gurez Highway. With NHPC Kishanganga Hydro Power Project Bandipore Jammu & Kashmir being in the vicinity and a new tunnel Razdan Pass Tunnel in the construction phase it becomes very important that stability of this slope is being assessed, for in many places over hundreds of meters the road is unprotected by the guardrails. Numerous tunnels have been constructed between 2007 and 2018 and also 37m tall concrete- face rock fill dam has been constructed during the construction of Kishanganga Hydro Power Project which adds to the already unstable slope which is snow covered for almost 6 months a year. Stability analysis of slopes using numerical analysis GEOSTUDIO is aimed at knowing about the factors that can potentially trigger a slope movement, understanding why a slope failure has occurred and, as well as the prevention of starting of such movement, at least slow it down or arrest it completely through mitigation countermeasures.
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Vallet, Anne-Laure. „Réactivités de NHC-Boranes Soufrés“. Thesis, Paris 11, 2014. http://www.theses.fr/2014PA112261.

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Avec le développement des concepts de la chimie verte, il est devenu nécessaire de remplacer les métaux toxiques par des composés plus respectueux de l’environnement. Donneurs d’hydrogène pour des réactions radicalaires, les hydrures de trialkylétain sont encore très utilisés. Les NHC-boranes se sont révélés de bons remplaçants pour les réactions de désoxygénation. Cependant, les réactions de déshalogénation effectuées avec des NHC-boranes sont moins efficaces et il a fallu avoir recours au concept de catalyse à polarité inversée. Ces travaux ont été le point de départ de cette thèse où la synthèse de nouveaux NHC-boranes possédant une liaison B-S ou B-N est développée. L’étude des propriétés de ces nouveaux complexes a été effectuée et des applications en chimie organique et en science des polymères ont été trouvées. Par ailleurs, en vue d’étudier les effets polaires sur la formation et la réactivité des radicaux boryles, une nouvelle famille de carbène-boranes a été synthétisée
Along with the development of green chemistry, it became necessary to avoid toxic metallic complexes in organic reactions and replace them by more sustainable compounds. An hydrogen donors for radical reactions, trialkylstannanes are still widely used. NHC-boranes seem to be good substitutes for deoxygenation reactions. However, dehalogenation reactions are less effective and polar reversal catalysis was used. This work was the starting point of this Ph.D thesis where the synthesis of new NHC-boranes bearing a B-S or B-N bound is developed. The study of the properties of these new complexes was performed and applications in organic chemistry as well as in polymer science were found. Besides, to study polar effects on the formation and on the reactivity of boryl radicals, a new family of carbene-boranes was synthesized
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Quinn, Peter. „Ortho-substituent effects in NHC-catalysis“. Thesis, Durham University, 2018. http://etheses.dur.ac.uk/12876/.

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N-Heterocyclic carbenes (NHCs) are extensively used in contemporary organocatalysis in diverse C-C bond forming reactions. Although successful, high catalyst loadings are typically required and chemoselectivity highly depends on catalyst type and structure. Catalysts are commonly selected by screening based approaches because it is not entirely clear how the structures of both the N-aryl ring and aldehyde substrate affect reactivity. In particular, ortho-substituents on both the NHC and aryl aldehyde have been found to display enhanced rate and equilibrium constants for deprotonation and hydroxyaryl adduct formation, however, the mechanistic reason is unclear. Chapter 2 describes the synthesis, kinetic evaluation and structural analysis using X-ray crystallography and DFT calculations of a range of triazolium salts, which are precursors to triazolyl NHCs. The findings from this chapter suggest that large dihedral angles between the triazolium and N-aryl ring, which are found in ortho-substituted systems, lead to relatively fast proton transfer from the triazolium salts. This is attributed to a ground state destabilisation of the cationic starting material through reduced inter-ring conjugation. Chapter 3 describes the kinetic studies aimed at investigating ortho-substituent effects on both the catalyst and aldehyde substrate on the intial hydroxyaryl adduct forming equilibrium. Ortho-substituted NHCs result in relatively fast rates and large equilibrium constants for hydroxyaryl adduct formation. This may be attributed to the relatively large dihedral angle between the triazolyl and N-aryl rings. Approach of the incoming electrophile requires the N-aryl ring to rotate out of plane of the triazolyl ring, which results in an energetic penalty for non-ortho-substituted NHCs. An ortho-alkoxy substituent on the aryl aldehyde have also been found to lead to enhanced rate and equilibrium constants for formation relative to unsubstitued aryl aldehyde. These findings are rationalised in terms of the formation of an increasingly optimal intramolecular H-bond in the transition state for hydroxyaryl adduct formation. Chapter 4 describes the attempts to determine a pKa for the conjugate acid of the Breslow intermediate based on the triazolyl scaffold. Methylated analogues were prepared and the kinetics of deprotonation by NMR and stopped flow UV-vis spectrophotometry are in agreement and suggest that a small proportion of O-methylated BI is observable in aqueous solution. Attempts were also made to reprotonate the BI to determine a pKa value in aqueous solution.
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Baffert, Mathieu. „Synthesis of ruthenium complexes having one or more N-heterocyclic carbene ligands supported on hybrid mesostructured silicas and their use in the hydrogenation of carbon dioxide“. Phd thesis, Université Claude Bernard - Lyon I, 2011. http://tel.archives-ouvertes.fr/tel-00838661.

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The goal of this PhD was to elaborate supported Ru-NHC catalytic materials based on hybrid organic-inorganic materials having imidazolium units perfectly distributed within a silica matrix. Passivation of these imidazolium materials followed by formation of NHC-carbene and reaction with [RuCl2(p-cymene)]2 provided these well-defined surface sites of general structures RuCl2(NHC)(L), where L was para-cymene (p-cymene) or THF depending on the reaction conditions, which could be further replaced by PMe3. These systems were then tested in the hydrogenation of CO2 in presence of amine to give formamides. The mono-NHC systems were highly active only in the presence of PMe3 ligands, but suffered from Ru leaching, evidencing the low stability of the NHC-Ru bond under the reaction conditions. On the other hand, dinuclear bis-NHC Ru systems were also developed, and they displayed much improved activity and stability in the hydrogenation of CO2 in the presence of PMe3 compared to the mono-NHC systems. This allowed the use of much higher reaction temperatures (200 °C) and provided heterogeneous catalysts with performances close to those obtained with the best homogeneous catalysts, Cl2Ru(dppe)2.
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Fauché, Kévin. „NHC portant des azotures : intermédiaires dans la synthèse catalysée d‘hétérocycles polyazotés et auto-fonctionnalisation de complexes métal-NHC“. Thesis, Université Clermont Auvergne‎ (2017-2020), 2018. http://www.theses.fr/2018CLFAC062/document.

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Les carbènes N-hétérocycliques (NHC) sont très utilisés pour complexer les métaux de transition. Ils quittent rarement ce rôle de ligand ancillaire et trouvent, depuis une vingtaine d’années, des applications en catalyse ou, plus récemment, en chimie médicinale. Dans ce travail, nous discuterons d’une méthode de synthèse douce conduisant à la formation de complexes AgI – NHC via une source d’argent soluble. Cette méthode nous a permis d’obtenir des complexes bien connus mais également d’accéder à une nouvelle série de complexes NHC-Ag-phosphine. Nous présenterons également une nouvelle réaction où des NHC portant une fonction azoture à proximité du carbone du carbène quittent leur rôle de ligand ancillaire et conduisent à la formation d’hétérocycles azotés par cyclisation carbène-nitrène. Cette réaction sera présentée en détail, ainsi que la caractérisation spectroscopique concernant une sous-série de composés fluorescents obtenus par cette méthode. Enfin, nous présenterons une stratégie de post-fonctionnalisation de complexes développée dans notre équipe. Des complexes argent(I)-NHC portant un azoture proches du centre carbénique catalysent leur propre fonctionnalisation. De plus, des complexes de cuivre(I) portant des azotures en position éloignée du centre métallique seront greffés sur des nanoparticules magnétiques pour servir de catalyseur recyclables
N-heterocyclic carbenes (NHC) are widely used to complex transition metals. They rarely leave their role as ancillary ligand and find, since 20 years, applications in catalysis or, more recently, in medicinal chemistry. In this work, we will discuss a mild synthetic method leading to the formation of AgI – NHC complexes via a soluble silver species. This method allowed us to obtain well known complexes but also to access a new series of NHC-Ag-phosphine complexes. We will also present a new reaction where NHC ligands bearing an azide function close to the carbenic center leave their role as ancillary ligand and lead to the formation of nitrogen rich heterocycles by a carbene-nitrene cyclization. This reaction will be presented in detail, along with the spectroscopic characterization regarding a sub-series of fluorescent compounds obtained by this method. Finally, we will present a post-functionalization strategy of complexes developed in our team. Silver(I)-NHC complexes tagged by an azide close to the carbenic center catalysed their own functionalization. Moreover, copper(I) complexes tagged by an azide function in a distant position from the metallic centre will be grafted on magnetic nanoparticles to act as recyclable catalysts
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Ma, Ruoyu. „Chiral NHC-Au(I) Catalyzed Enantioselective Reactions“. Miami University / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=miami1585432603722902.

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Brenet, Simon. „Nouvelle structure BINOL-Maléimide. Applications en catalyse d'oxydation asymétrique“. Thesis, Grenoble, 2013. http://www.theses.fr/2013GRENV050/document.

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Les réactions asymétriques catalysées constituent une approche élégante au problème de l'introduction de centres stéréogène pour la synthèse de molécules complexes. Dans ce contexte, les oxydations sont notables de par la possibilité de créer en une seule étape à la fois le centre asymétrique ainsi que les fonctions dont les réactivités sont à la base des méthodologies de synthèse. La sélectivité d'un catalyseur est le plus souvent contrôlée par sa structure même. Notre groupe étudie les oxydations aérobies asymétriques par activation C-H par des analogues chiraux du N-hydroxyphtalimide (NHPI). Nous avons développé une nouvelle structure de catalyseur comportant un cœur BINOL-maléimide afin de dépasser les limitations intrinsèque des précédentes générations de catalyseurs. La première série n'a pas montré d'énantiosélectivité, cela nous a conduit a synthétiser une deuxième série dont les caractéristiques permettraient d'améliorer l'efficacité de ces catalyseurs d'oxydation aérobie asymétrique. Le développement d'un analogue portant deux iodes nous a conduit à étudier cette structure en tant que cœur de catalyseurs à base d'iode hypervalent. Nous avons analysé trois séries de ces catalyseurs pour l'oxytosylation de cétones prochirales et montré que notre structure est comparable avec celles présentées dans la littérature
Catalysed asymmetric reactions are an elegant approach towards the synthesis of complex molecules for the controlled introduction of stereocenters. Within this field, oxidations are noteworthy since they allow to create in one step both the stereogenic elements and the moieties whose reactivities are the cornerstone of the methodologies of synthesis. The selectivity of a catalyst is most often controlled by its own structure. Our group has investigated catalysed asymmetric aerobic oxidations by C-H activation through the use of chiral analogues of N-hydroxyphtalimide (NHPI). We have developed a new structure of catalyst based upon a BINOL-fused maleimide core to circumvent the inherent limitations of previous catalysts generations. The first series showed no enantioselectivity and led us to synthesize a second series whose features are expected to improve the overall efficiency of those catalysts for asymmetric aerobic oxidations. The development of an analogue bearing two iodine moieties prompted us to study this structure as a core for chiral hypervalent organo-iodine(lll) catalysts. We have investigated three series of such catalysts for the asymmetric α-oxytosylation of prochiral ketones and showed that our structure is comparable with the ones found in the literature
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Saker, Oliver John. „Mono- and tri-nuclear ruthenium complexes incorporating N-heterocyclic carbene ligands“. Thesis, University of Bath, 2008. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.512305.

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Giavara, Sabrina. „Functional analysis of Nhp6, Tel1 and Xrs2 proteins in Saccharomyces cerevisiae“. Thesis, University of Cambridge, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.614868.

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VIDEIRA, Marco André Moras. „Estudo dos antiporters Na+/H+ NhaC e NhaE de Neisseria meningitidis“. Master's thesis, Instituto de Higiene e Medicina Tropical, 2013. http://hdl.handle.net/10362/19208.

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Os antiporters Na+/H+ são proteínas membranares, encontradas em todos os reinos e envolvidas na homeostasia do Na+ e do pH. Dois antiporters Na+/H+, denominados NmNhaC e NmNhaE foram identificados no genoma da bactéria patogénica, Neisseria meningitidis, e estudados através da sua expressão heteróloga numa estirpe de E. coli deficiente em antiporters Na+/H+, a estirpe E. coli KNabc. Os antiporters Na+/H+ de N. meningitidis, foram capazes de conferir resistência ao Na+, a esta estirpe, sugerindo que os dois possam contribuir para a homeostasia do Na+ e pH. O NmNhaE é também capaz de transportar Li+, e parece ser o principal antiporter Na+/H+ a contribuir para este papel em N. meningitidis. O NmNhaE tem atividade de antiporter Na+/H+ entre valores de pH 6.5 a 9, e a atividade Na+(Li+)/H+ ótima é observada a pH 7. Além disso, verificou-se que o NmNhaE tem maior afinidade para o Na+. Com o objetivo de encontrar resíduos importantes na função e estrutura do NmNhaE, dez resíduos de aminoácidos foram selecionados apartir de comparações de alinhamentos entre sequências de aminoácidos, e também através de estudos anteriores de mutagénese dirigida noutros antiporters Na+/H+, tendo as mutações pontuais sido intruzidas no gene nhaE, resultando nas mutações E91Q, H161L, G174S, D182N, H201L, V258T, H272L, H356L, H361L e G413S. Quando a expressão dos mutantes foi induzida em E. coli KNabc, todos os mutantes, com exceção dos mutantes G174S e H201L, foram capazes de complementar a estirpe KNabc, permitindo que esta crescesse em meio LBK líquido com NaCl 500 mM. Este facto sugere que as mutações G174S e H201L podem impedir a atividade de antiporter Na+/H+. Nos mutantes E91Q, V258T e H361L o Km determinado a pH 7, para o Na+, é semelhante ao determinado no NhaE selvagem, contudo o seu Vmax sofreu uma descida de quase duas vezes. Além disso, a atividade medida a pH 7 para os mutantes V258T e E91Q, e a atividade medida a 7.5 para o mutante V258T, diminuiu relativamente ao NhaE selvagem. Este resultado juntamente com as atividades medidas a pH 7 e 7.5, sugerem que os resíduos E91, V258, H361 estejam envolvidos na resposta ao pH. No mutante H272L, verificou-se um aumento no Km determinado para o Na+ a pH 7 para quase o dobro, enquanto o Vmax se manteve inalterado em relação ao NhaE selvagem. Os parâmetros cinéticos e as atividades medidas a pH 7 e 7.5 sugerem que o resíduo H272 pode estar envolvido no transporte de Na+ e também na resposta ao pH. Nos restantes residuos não se verificou atividade de antiporter Na+/H+. Este trabalho contribuiu para uma melhor compreensão do transporte de Na+ nos antiporters Nha de N. menigitidis, nomeadamente no funcionamento e estrutura do NhaE. No futuro, este trabalho pode fornecer informação relevante quanto ao papel do Na+ na infeção e sobrevivência desta bactéria no hospedeiro.
Na+/H+ antiporters are membrane proteins present in all kingdoms involved in Na+ and pH homeostasis. Two Na+/H+ antiporters, NmNhaC and NmNhaE, from the pathogenic bacterium, Neisseria meningitis, were identified in the genome and studied through heterologous expression in the E. coli KNabc, a strain lacking the major Na+/H+ antiporters. N. meningitidis Na+/H+ antiporters, were able to restore Na+ resistance to E. coli KNabc, suggesting that they may contribute to the Na+ and pH homeostasis. NmNhaE is also able to transport Li+ ions and appears to be the main Na+/H+ antiporter contributing to this role in N. meningitidis. The Na+/H+ antiporter activity of NmNhaE is active between pH 6.5 to 9, the optimal Na+(Li+)/H+ antiporter activity being observed at pH 7. Furthermore, NmNhaE displayed higher affinity towars Na+. To address important functional and structural residues in NmNhaE, ten amino acid residues were selected from sequence alignments comparisions and previous studies of site directed mutagenesis in other Na+/H+ antiporters and point mutations were introduced in the nhaE gene, resulting in E91Q, H161L, G174S, D182N, H201L, V258T, H272L, H356L, H361L and G413S mutations. When their expression was induced, all mutants but G174S and H201L, allowed E. coli KNabc strain it to grow in LBK with NaCl 500 mM. This suggests that mutations G174S and H201L may block the antiporter activity. No alteration was seen regarding the Km measured for Na+ at pH 7 of mutants E91Q, V258T and H361L when compared to the NhaE wild type, however their Vmax suffered a decrease of almost two times. In addition, the activities measured at pH 7 and 7.5 showed a decrease in V258T, while E91Q showed a decrease in activity at pH 7, when compared with the NhaE wild type. The determined kinetic parameters and the measured activities suggests that residues E91, V258, H361 may be involved in the pH response. The comparision between the NhaE kinetic parameters with those of the recombinant H272L showed almost one fold increase in the Km for Na+ at pH 7, while the Vmax showed no difference. The kinetic parameters and the activities measured at pH 7 and pH 7.5 suggests that residue H272 may be involved in the translocation of Na+ and also in pH response. No Na+/H+ antiporter activity was observed in the remaining residues. This work contributed for a better understanding of Na+ transport in N. meningitidis Nha Na+/H+ antiporters namely the function of Na+/H+ antiporters and important residues to their function. In the future, this work can provide useful information about the role of Na+ in the infection and survival of this bacterium in the host.
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Bücher zum Thema "NHPC"

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Hò̂, Trà̂n Phương. Tây Sơn tam kiệt. [Hà Nội]: Nhà xuá̂t bản Văn học, 1997.

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Council, National House-Building. NHBC standards. Amersham: National House-Building Council, 1991.

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Trần, Vinh. Nhạc chèo. Hà Nội: Nhà xuất bản Sân khấu, 2011.

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Council, National House-Building. NHBC standards. Amersham: National House-Building Council, 1990.

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Council, National House-Building. NHBC standards. Amersham: National House-Building Council, 1999.

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Council, National House-Building. NHBC standards. Amersham: National House-Building Council, 1991.

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Council, National House-Building. NHBC standards. Amersham: National House-Building Council, 1990.

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Council, National House-Building. NHBC standards. Amsterdam: National House-Building Council, 1992.

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National Health Service Corps (U.S.), Hrsg. NHSC professional policies. Rockville, Md: U.S. Dept. of Health & Human Services, Public Health Service, Health Resources and Services Administration, Bureau of Health Care Delivery and Assistance, National Health Service Corps, 1985.

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Oánh, Thtam. Nhạc thxoi gian. Falls Church, VA: Minh Văn, 1995.

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Buchteile zum Thema "NHPC"

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Peter, Helga. „NHP“. In Springer Reference Medizin, 1. Berlin, Heidelberg: Springer Berlin Heidelberg, 2020. http://dx.doi.org/10.1007/978-3-642-54672-3_695-1.

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Hofmann, Peter, und Marcel Brill. „NHCP Ligands for Catalysis“. In Molecular Catalysts, 207–34. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2014. http://dx.doi.org/10.1002/9783527673278.ch10.

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Dashti, Amir, Vidisha Singh und Ann Chahroudi. „Designing Cure Studies in NHPs“. In Methods in Molecular Biology, 215–28. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-1871-4_16.

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Hanagal, David D., und Nileema N. Bhalerao. „NHPP Software Reliability Growth Models“. In Infosys Science Foundation Series, 27–37. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-16-0025-8_3.

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Schlepphorst, Christoph. „Einleitung“. In Ruthenium-NHC-katalysierte asymmetrische Arenhydrierung, 1–24. Wiesbaden: Springer Fachmedien Wiesbaden, 2015. http://dx.doi.org/10.1007/978-3-658-08967-2_1.

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Schlepphorst, Christoph. „Synthese neuartiger NHCs“. In Ruthenium-NHC-katalysierte asymmetrische Arenhydrierung, 25–42. Wiesbaden: Springer Fachmedien Wiesbaden, 2015. http://dx.doi.org/10.1007/978-3-658-08967-2_2.

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Schlepphorst, Christoph. „Asymmetrische Hydrierung von Pyrazinen“. In Ruthenium-NHC-katalysierte asymmetrische Arenhydrierung, 43–50. Wiesbaden: Springer Fachmedien Wiesbaden, 2015. http://dx.doi.org/10.1007/978-3-658-08967-2_3.

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Schlepphorst, Christoph. „Zusammenfassung und Ausblick“. In Ruthenium-NHC-katalysierte asymmetrische Arenhydrierung, 51. Wiesbaden: Springer Fachmedien Wiesbaden, 2015. http://dx.doi.org/10.1007/978-3-658-08967-2_4.

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Schlepphorst, Christoph. „Experimenteller Teil“. In Ruthenium-NHC-katalysierte asymmetrische Arenhydrierung, 53–70. Wiesbaden: Springer Fachmedien Wiesbaden, 2015. http://dx.doi.org/10.1007/978-3-658-08967-2_5.

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Teixeira-Salmela, Luci Fuscaldi. „Nottingham Health Profile (NHP)“. In Encyclopedia of Quality of Life and Well-Being Research, 4388–91. Dordrecht: Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-007-0753-5_1969.

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Konferenzberichte zum Thema "NHPC"

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BNS Naveen, Kumar, und Sahoo Radhendra Narayan. „Successful excavation of Surge Shaft in weak rock mass: A case study of 240MW URI-II HE Project, NHPC, JaK“. In Recent Advances in Rock Engineering (RARE 2016). Paris, France: Atlantis Press, 2016. http://dx.doi.org/10.2991/rare-16.2016.29.

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Haque, Md Asraful, und Nesar Ahmad. „An Imperfect SRGM based on NHPP“. In 2021 Third International Conference on Inventive Research in Computing Applications (ICIRCA). IEEE, 2021. http://dx.doi.org/10.1109/icirca51532.2021.9544959.

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Araujo, Elinaldo B. „Using NHPP to Evaluate QMS Reliability Growth“. In 2019 Annual Reliability and Maintainability Symposium (RAMS). IEEE, 2019. http://dx.doi.org/10.1109/rams.2019.8769003.

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Yadati, Naganand, Vikram Nitin, Madhav Nimishakavi, Prateek Yadav, Anand Louis und Partha Talukdar. „NHP: Neural Hypergraph Link Prediction“. In CIKM '20: The 29th ACM International Conference on Information and Knowledge Management. New York, NY, USA: ACM, 2020. http://dx.doi.org/10.1145/3340531.3411870.

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Duy, Phan Đình, Trần Ngọc Đức, Trần Văn Quang und Vũ Đức Lung. „HỆ THỐNG ĐIỀU KHIỂN MA TRẬN PHUN NƯỚC NGHỆ THUẬT THEO NHẠC DỰA TRÊN BỘ PHÂN LỚP ÂM NHẠC“. In NGHIÊN CỨU CƠ BẢN VÀ ỨNG DỤNG CÔNG NGHỆ THÔNG TIN. Publishing House for Science and Technology, 2017. http://dx.doi.org/10.15625/vap.2016.00028.

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Yang Zhihong und Liu Cihua. „Estimating parameters of Ohba three-parameters NHPP Models“. In 2011 International Conference on Computer Science and Service System (CSSS). IEEE, 2011. http://dx.doi.org/10.1109/csss.2011.5974854.

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Nicol, David M., und Lawrence M. Leemis. „A continuous piecewise-linear NHPP intensity function estimator“. In 2014 Winter Simulation Conference - (WSC 2014). IEEE, 2014. http://dx.doi.org/10.1109/wsc.2014.7019915.

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Nagaraju, Vidhyashree, und Lance Fiondella. „An adaptive EM algorithm for NHPP software reliability models“. In 2015 Annual Reliability and Maintainability Symposium (RAMS). IEEE, 2015. http://dx.doi.org/10.1109/rams.2015.7105160.

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Garmabaki, A. H. S., A. Barabadi, F. Yuan, J. Lu und Y. Z. Ayele. „Reliability modeling of successive release of software using NHPP“. In 2015 IEEE International Conference on Industrial Engineering and Engineering Management (IEEM). IEEE, 2015. http://dx.doi.org/10.1109/ieem.2015.7385750.

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Gonzalez, Camila A., Alvaro Torres und Mario A. Rios. „Reliability assessment of distribution power repairable systems using NHPP“. In 2014 IEEE PES Transmission & Distribution Conference and Exposition - Latin America (PES T&D-LA). IEEE, 2014. http://dx.doi.org/10.1109/tdc-la.2014.6955225.

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Berichte der Organisationen zum Thema "NHPC"

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NAVAL HEALTH RESEARCH CENTER SAN DIEGO CA. NHRC Update, Issue No.8. Fort Belvoir, VA: Defense Technical Information Center, Januar 1998. http://dx.doi.org/10.21236/ada339064.

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NAVAL HEALTH RESEARCH CENTER SAN DIEGO CA. NHRC Update, Issue No. 7. Fort Belvoir, VA: Defense Technical Information Center, Oktober 1997. http://dx.doi.org/10.21236/ada339073.

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NAVAL HEALTH RESEARCH CENTER SAN DIEGO CA. NHRC Update Issue No. 10. Fort Belvoir, VA: Defense Technical Information Center, Juli 1998. http://dx.doi.org/10.21236/ada353687.

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Bush, Ruth A., Janet L. Dickeson und William E. Hamilton. An Overview of NHRC Medical Engineering Process. Fort Belvoir, VA: Defense Technical Information Center, Oktober 2006. http://dx.doi.org/10.21236/ada462166.

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Carlson, R., und L. Winkler. Guidelines for Next Hop Client (NHC) Developers. RFC Editor, Mai 1999. http://dx.doi.org/10.17487/rfc2583.

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Surdyk, Shelby, Carolyn Furbish, Jennifer Larsen und Mary Hake. Amphibian surveys at Klondike Gold Rush NHP: 2019 summary. National Park Service, Juli 2022. http://dx.doi.org/10.36967/2293389.

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Story, Madison, Adam Smith und Sunny Adams. Fort McCoy firing ranges and military training lands : a history and analysis. Engineer Research and Development Center (U.S.), September 2022. http://dx.doi.org/10.21079/11681/45600.

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The US Congress codified the National Historic Preservation Act of 1966 (NHPA), the nation’s most effective cultural resources legislation to date, mostly through establishing the National Register of Historic Places (NRHP). The NHPA requires Federal agencies to address their cultural resources, which are defined as any prehistoric or historic district, site, building, structure, or object. Section 110of the NHPA requires Federal agencies to inventory and evaluate their cultural resources, and Section 106 requires them to determine the effect of Federal undertakings on those potentially eligible for the NRHP. Fort McCoy is entirely within Monroe County in west-central Wisconsin. It was first established as the Sparta Maneuver Tract in 1909.The post was renamed Camp McCoy in 1926. Since 1974, it has been known as Fort McCoy. This report provides a historic context for ranges, features, and buildings associated with the post’s training lands in support of Section 110 of the NHPA.
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Kanan, Matthew W. Local Electric Field Effects on Rhodium-Porphyrin and NHC-Gold Catalysts. Fort Belvoir, VA: Defense Technical Information Center, Januar 2015. http://dx.doi.org/10.21236/ad1013216.

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Adams, Sunny, Madison Story und Adam Smith. Evaluation of 11 buildings in the Fort McCoy cantonment. Engineer Research and Development Center (U.S.), September 2022. http://dx.doi.org/10.21079/11681/45350.

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The United States Congress codified the National Historic Preservation Act of 1966 (NHPA), the nation’s most effective cultural resources legislation to date, mostly through establishing the National Register of Historic Places (NRHP). The NHPA requires federal agencies to address their cultural resources, which are defined as any prehistoric or historic district, site, building, structure, or object. Section 110 of the NHPA requires federal agencies to inventory and evaluate their cultural resources, and Section 106 requires them to determine the effect of federal undertakings on those potentially eligible for the NRHP. Fort McCoy is in west-central Wisconsin, entirely within Monroe County. It was first established as the Sparta Maneuver Tract in 1909. The post was renamed Camp McCoy in 1926. Since 1974, it has been known as Fort McCoy. This report provides historic context and determinations of eligibility for buildings in the cantonment constructed between 1946 and 1975 and concludes that none are eligible for the NRHP. In consultation with the Wisconsin State Historic Preservation Officer (WISHPO), this work fulfills Section 110 requirements for these buildings.
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Story, Madison, und Adam Smith. Fort Hunter Liggett : a history and analysis. Engineer Research and Development Center (U.S.), Januar 2023. http://dx.doi.org/10.21079/11681/46340.

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The US Congress codified the National Historic Preservation Act of 1966 (NHPA), the nation’s most effective cultural resources legislation to date, mostly through establishing the National Register of Historic Places (NRHP). The NHPA requires Federal agencies to address their cultural resources, which are defined as any prehistoric or historic district, site, building, structure, or object. Section 110 of the NHPA requires Federal agencies to inventory and evaluate their cultural resources, and Section 106 requires them to determine the effect of Federal undertakings on those potentially eligible for the NRHP. Fort Hunter Liggett is located on California’s Central Coast within Monterey County. The fort has been used as a training facility for large-scale maneuvers and live-fire exercises since its establishment as a US Army training facility in 1941. The periods of significance for Criterion A are: from 1769 to 1833, relating to the founding and development of Mission San Antonio de Padua; from 1834 to 1923, relating to Euro-American land grants and ranchos; from 1923 to 1940, relating to Hearst’s purchase of the property and subsequent development; from 1940 to 1945, relating to the establishment of the Hunter Liggett Military Reservation (HLMR) and activities related to WWII; from 1959 to 1970, relating to the establishment and buildup of CDEC; and from 1975 to 1980, relating to HLMR’s redesignation as Fort Hunter Liggett and associated development. This report provides a comprehensive historic context for ranges, features, and buildings at Fort Hunter Liggett in support of Section 110 of the NHPA.
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