Thematische Bibliographien / Perfusable

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Auswahl der wissenschaftlichen Literatur zum Thema „Perfusable“

Autor: Grafiati

Veröffentlicht am 4. Juni 2021

Zuletzt aktualisiert am 12. Juni 2025

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Zeitschriftenartikel zum Thema "Perfusable"

Forgacs, Gabor. "Perfusable vascular networks." Nature Materials 11, no. 9 (2012): 746–47. http://dx.doi.org/10.1038/nmat3412.

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Fong, EL, M. Santoro, MC Farach-Carson, FK Kasper, and AG Mikos. "Tissue engineering perfusable cancer models." Current Opinion in Chemical Engineering 3 (February 2014): 112–17. http://dx.doi.org/10.1016/j.coche.2013.12.008.

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Tran, Reginald, Byungwook Ahn, David R. Myers, et al. "Simplified prototyping of perfusable polystyrene microfluidics." Biomicrofluidics 8, no. 4 (2014): 046501. http://dx.doi.org/10.1063/1.4892035.

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Liu, Juan, Huaiyuan Zheng, Patrina Poh, Hans-Günther Machens, and Arndt Schilling. "Hydrogels for Engineering of Perfusable Vascular Networks." International Journal of Molecular Sciences 16, no. 7 (2015): 15997–6016. http://dx.doi.org/10.3390/ijms160715997.

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Štumberger, Gabriela, and Boštjan Vihar. "Freeform Perfusable Microfluidics Embedded in Hydrogel Matrices." Materials 11, no. 12 (2018): 2529. http://dx.doi.org/10.3390/ma11122529.

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We report a modification of the freeform reversible embedding of suspended hydrogels (FRESH) 3D printing method for the fabrication of freeform perfusable microfluidics inside a hydrogel matrix. Xanthan gum is deposited into a CaCl2 infused gelatine slurry to form filaments, which are consequently rinsed to produce hollow channels. This provides a simple method for rapid prototyping of microfluidic devices based on biopolymers and potentially a new approach to the construction of vascular grafts for tissue engineering.

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Xu, Peidi, Ruoxiao Xie, Yupeng Liu, Guoan Luo, Mingyu Ding, and Qionglin Liang. "Bioinspired Microfibers with Embedded Perfusable Helical Channels." Advanced Materials 29, no. 34 (2017): 1701664. http://dx.doi.org/10.1002/adma.201701664.

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He, Jiankang, Lin Zhu, Yaxiong Liu, Dichen Li, and Zhongmin Jin. "Sequential assembly of 3D perfusable microfluidic hydrogels." Journal of Materials Science: Materials in Medicine 25, no. 11 (2014): 2491–500. http://dx.doi.org/10.1007/s10856-014-5270-9.

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Zhang, Yahui, Yin Yu, Adil Akkouch, Amer Dababneh, Farzaneh Dolati, and Ibrahim T. Ozbolat. "In vitro study of directly bioprinted perfusable vasculature conduits." Biomaterials Science 3, no. 1 (2015): 134–43. http://dx.doi.org/10.1039/c4bm00234b.

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Bogorad, Max I., Jackson DeStefano, Johan Karlsson, Andrew D. Wong, Sharon Gerecht, and Peter C. Searson. "Review: in vitro microvessel models." Lab on a Chip 15, no. 22 (2015): 4242–55. http://dx.doi.org/10.1039/c5lc00832h.

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Campbell, Rachel, Karina A. Hernandez, Tatiana Boyko, et al. "Fabrication of perfusable microvessels within tissue engineered constructs." Journal of the American College of Surgeons 217, no. 3 (2013): S143—S144. http://dx.doi.org/10.1016/j.jamcollsurg.2013.07.337.

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Dissertationen zum Thema "Perfusable"

Whisler, Jordan Ari. "Engineered, functional, human microvasculature in a perfusable fluidic device." Thesis, Massachusetts Institute of Technology, 2017. http://hdl.handle.net/1721.1/113761.

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Thesis: Ph. D., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2017.
Cataloged from PDF version of thesis.
Includes bibliographical references (pages 141-154).
Engineered, human tissue models will enable us to study disease more accurately, and develop treatments more economically, than ever before. Functional tissue grown in the laboratory will also provide a much-needed source for the clinical replacement of diseased or damaged tissues. A major hindrance to the development of these technologies has been the inability to vascularize tissue-engineered constructs, resulting in limited size and biological complexity. In this thesis, we report the development of a novel 3D fluidic platform for the generation of functional, human, microvasculature. Using different fabrication methods, we developed both a micro-fluidic system (0.1 - 1 mm tissue dimensions) - used for high throughput disease modeling assays, and a meso-fluidic system (I - 10 mm tissue dimensions) - for generating removable tissue-engineered constructs. These systems were validated by their successful use in a metastasis model - to elucidate the mechanism of cancer cell extravasation, and in the formation of a vascularized, perfusable tissue construct containing pancreatic islets, respectively. Vascularization, in our system, was achieved by encapsulating endothelial cells in a 3D fibrin matrix and relying on their inherent ability to collectively self-assemble into a functional vasculature - as they do during embryonic development. To better understand and characterize this process, we measured the morphological, functional, mechanical, and biological properties of the tissue as they emerged during vascular morphogenesis. We found that juxtacrine interactions between endothelial cells and fibroblasts enhanced the functionality and stability of the newly formed vasculature - as characterized via vascular permeability and gene expression. Under optimal co-culture conditions, the tissue stiffness increased 10- fold, mainly due to organized cellular contraction. Additionally, over the course of 2-weeks, the cells deposited over 50 new extracellular matrix (ECM) proteins, accounting for roughly 1/3 of the total ECM. These results shed light on the mechanisms underlying vascular morphogenesis and will be useful in further developing vascularization strategies for tissue engineering and regenerative medicine applications. Key words: Tissue Engineering, Vascularization, Microfluidics, In Vitro Model.
by Jordan Ari Whisler.
Ph. D.

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Whisler, Jordan Ari. "Engineered, perfusable, human microvascular networks on a microfluidic chip." Thesis, Massachusetts Institute of Technology, 2013. http://hdl.handle.net/1721.1/85772.

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Thesis: S.M., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2013.
Cataloged from PDF version of thesis.
Includes bibliographical references (pages 61-64).
In this thesis, we developed a reliable platform for engineering perfusable, microvascular networks on-demand using state of the art microfluidics technology. We have demonstrated the utility of this platform for studying cancer metastasis and as a test bed for drug discovery and analysis. In parallel, this platform enabled us to study, in a highly controlled environment, the physiologic processes of angiogenesis and vasculogenesis to further elucidate their underlying mechanisms. In addition to using our platform for real-time observation of physiological processes, we also took advantage of the ability to influence these processes through precise control of the extracellular environment. By manipulating the mechanical and bio-chemical inputs to our system, we controlled the dynamics of microvascular network formation as well as key properties of the network morphology. These findings will aid in the design and engineering of organ specific constructs for tissue engineering and regenerative medicine applications. Finally, we explored the potential use of stem cells for engineering microvascular networks in our system. We found that human mesenchymal stem cells can act as secondary, support cells during microvascular network formation.
by Jordan Ari Whisler.
S.M.

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Sphabmixay, Pierre. "Engineering micro-perfusable scaffolds for MesoPhysiological Systems using projection Micro-StereoLithography." Thesis, Massachusetts Institute of Technology, 2020. https://hdl.handle.net/1721.1/129115.

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Thesis: Ph. D., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2020
Cataloged from student-submitted PDF of thesis.
Includes bibliographical references (pages 140-155).
MicroPhysiological Systems (MPS) are in vitro models that capture the complexity of human organs at miniature scale by recreating the native microenvironment of resident cells. These systems offer promising alternatives to in vivo animal models for the development of new drugs, disease modeling and biological research. The organs in the human body are continuously perfused via a dense network of blood vessels delivering oxygen, nutrients and biomolecules locally while clearing waste materials produced by the tissue. As a result, MPS that incorporate microperfusion in a three-dimensional format have been a major focal point in the community driving major efforts towards in vitro vascularization methods. A major obstacle to the development of these MPS was the micrometric scale of the human cells forming the building block of any biological system.
But advances in micro and nanofabrication techniques have led to the creation of a myriad of new MPS that allow the successful culture of 3D tissues under microperfusion. Nevertheless, the translation of in vitro data from MPS to clinical data is confronted with the fundamental problem arising from the multi-dimensional scaling of experimental parameters, from micrometric systems to macroscale organs. This thesis describes the design, fabrication and implementation of a MesoPhysiological System (MePS) for the culture of human cells at mescoscopic scale. The MePS consists of a perfusable 3D printed network of microcapillaries serving as a scaffold for the tissue with built-in vasculature. The manufacturing of the MePS was performed using a Projection Micro-StereoLithography Apparatus which enabled the fabrication of centimetric scaffolds with micrometric features at high through-put.
The geometry of the MePS was carefully designed using computational fluid dynamics and computational model of oxygen transport so that critical physico-chemical parameters of the MePS, such as shear forces and oxygen levels would reach physiological values. Long term cultures of liver and brain tissues were performed in the MePS and featured elevated function and viability compared to other MPS. The increased metabolic rate and hepatic function of the liver MePS permitted to recapitulate critical features of metabolic disorders, such as chronic development of an insulin resistance phenotype in type 2 diabetes mellitus.
by Pierre Sphabmixay.
Ph. D.
Ph.D. Massachusetts Institute of Technology, Department of Mechanical Engineering

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Salameh, Sacha. "A perfusable vascularized full thickness skin model for topical and systemic applications." Thesis, Sorbonne université, 2019. http://www.theses.fr/2019SORUS466.

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De nombreux progrès ont déjà été réalisés pour développer des modèles de peau in vitro plus complexes et s’approchant de plus en plus du tissu in vivo. Cependant, la vascularisation reste l’un des défis à relever pour l’évolution des peaux reconstruites. Notre étude s'est concentrée sur le développement d'un équivalent de peau complète vascularisée et perfusée avec un réseau vasculaire plus complexe que les modèles déjà existants. Ainsi, nous avons combiné trois techniques : le moulage de la matrice, l’auto-assemblage des cellules endothéliales et la microfluidique. Ainsi, nous avons créé un équivalent de peau vascularisée constituée i) d’un épiderme différencié avec une organisation physiologique ii) de trois macrovaisseaux perfusables avec des bourgeonnements formés par angiogenèse, iii) d’un réseau microvasculaire créé par vasculogenèse et connecté aux bourgeons des macrovaisseaux. Les évaluations de l’absorption percutanée et de la diffusion systémique de composés ont démontré qu’avoir un système vasculaire perfusable et plus proche du plexus vasculaire in vivo aboutit à un modèle plus prédictif pour les évaluations d’applications topiques ou systémiques de composés. Cela inclut une large gamme d'utilisations du modèle, soit pour la connaissance, soit pour des études d'efficacité et de sécurité
There is still an unmet need of vascularized in vitro skin models mimicking human skin that could be faithfully used as an alternative to in vivo or ex vivo testing for efficacy and safety studies. Indeed, vascularization and perfusion are still two of the main remaining challenges in skin models. Our study was therefore aimed at developing a perfusable vascularized full thickness skin equivalent with a more complex blood vasculature compared to existing models. We here combined molding, auto-assembly and microfluidics techniques in order to produce a skin equivalent that recapitulates a properly differentiated epidermis and also complex vascular networks connected together in the dermal equivalent. Three perfusable vascular channels that sprouted via angiogenesis were created and eventually connected to a microvascular network, generated by endothelial cells auto-assembly, i.e. vasculogenesis. We then evaluated skin permeability of various compounds with different chemical properties and systemic delivery of a pollutant (Benzo[a]pyrene) and demonstrated that perfusion of in vivo-like vascular plexus resulted in more predictive and reliable model for topical and systemic assessments. This model could therefore further drug discovery and improve clinical translation in dermatology

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Entz, Michael William II. "Effects of Perfusate Solution Composition on the Relationship between Cardiac Conduction Velocity and Gap Junction Coupling." Diss., Virginia Tech, 2018. http://hdl.handle.net/10919/81823.

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Reproducibility of results in biomedical research is an area of concern that should be paramount for all researchers. Importantly, this issue has been examined for experiments concerning cardiac electrophysiology. Specifically, multiple labs have found differences in results when comparing cardiac conduction velocity (CV) between healthy mice and mice that were heterozygous null for the gap junction (GJ) forming protein, Connexin 43. While the results of the comparison study showed differing extracellular ionic concentrations of the perfusates, specifically sodium, potassium, and calcium ([Na+]o, [K+]o, and [Ca2+]o), there was a lack of understanding why certain combinations of the aforementioned ions led to specific CV changes. However, more research from our lab indicates that these changes can predict modifications to a secondary form of cardiac coupling known as ephaptic coupling (EpC). Therefore the work in this dissertation was twofold, 1) to examine the effects of modulating EpC through perfusate ionic concentrations while also modulating GJC and 2) to investigate the effects of modulating all three of the main ions contributed with cardiac conduction (Na+, K+, Ca2+) and the interplay between them. Firstly I designed and tested changes from the use of 3D printed bath for optical mapping procedures. After verification that the bath did not modify electrophysiological or contrile parameters, I studied the effects of physiologic changes to EpC determinants ([Na+]o and [K+]o) on CV during various states of GJ inhibition using the non-specific GJ uncoupler carbenoxolone (CBX). Multiple pacing rates were used to further modify EpC, as an increased pacing rate leads to a decrease in sodium channel availability through modification of the resting membrane potential. with no to low (0 and 15 µM CBX) GJ inhibition, physiologic changes in [Na+]o and [K+]o did not affect CV, however increasing pacing rate decreased CV as expected. When CBX was increased to 30 µM, a combination of decreasing [Na+]o and increasing [K+]o significantly decreased cardiac CV, specifically when pacing rate was increased. Next, the combinatory effects of cations associated with EpC (Na+, K+, and Ca2+) were tested in to examine how cardiac CV reacts to changes in perfusate solution and how this may explain differences in experimental outcomes between laboratories. Briefly, experiments were run where [K+]o was varied throughout an experiment and the values for [Na+]o and [Ca2+]o were at one of two specific values during an experiment. 30 µM CBX was added to half of the experiments to see the changes in the CV-[K+]o relationship with GJ inhibition. With unaltered GJ coupling, elevated [Na+]o maintains CV during hyperkalemia. Interestingly, both [Na+]o and [Ca2+]o must be increased to maintain normal CV during hyperkalemia with reduced GJ coupling. These data suggest that optimized fluids can sustain normal conduction under pathophysiologic conditions like hyperkalemia and GJ uncoupling. Taken as a whole, this dissertation attempts to shed light on the importance of ionic concentration balance in perfusate solutions on cardiac conduction.
Ph. D.

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Webster, Kelly Eileen. "Quantifying Renal Swelling during Machine Perfusion using Digital Image Correlation." Thesis, Virginia Tech, 2017. http://hdl.handle.net/10919/78244.

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While machine perfusion of explanted kidneys is theoretically superior to standard cold storage, it may damage potential transplants unless machine-associated swelling is controlled. This thesis presents the effects of perfusate tonicity on renal swelling during hypothermic machine perfusion. Phosphate buffered solution (PBS) and PBS supplemented with 5% w/v mannitol were used as isotonic (289 mOsm/kg) and hypertonic (568 mOsm/kg) perfusates, respectively. Porcine kidney pairs were procured then flushed and machine perfused; the right and left kidneys were assigned opposite perfusates. An experimental methodology was developed to image porcine kidneys undergoing hypothermic machine perfusion (5 deg C) for 15 minutes followed by 120 minutes without perfusion to quantify surface displacement (renal swelling) with digital image correlation (DIC). Surface displacement and size (thickness) were compared between the right and left kidneys of each pair. In addition, discharged renal fluids (i.e., filtrate and venous outflow) and biopsies were collected. On average, kidneys perfused with the mannitol solution were smaller in size than the kidneys perfused with PBS (p < 0.05) at the start and end of each experiment; however, there was no significant difference between the renal sizes at the end of the 15 minute perfusion interval (p > 0.05). Thus, hypertonic and isotonic perfusates yielded different renal swelling outcomes (i.e., physical size and surface displacement), which suggests that perfusate tonicity influences renal swelling. These experiments are the first time ex vivo renal surface displacement measurements have been collected during machine perfusion.
Master of Science

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Street, Darrin. "Skeletal Muscle Interstitium and Blood pH at Rest and During Exercise in Humans." Queensland University of Technology, 2003. http://eprints.qut.edu.au/15850/.

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The aims of this thesis were to: 1) develop a new method for the determination of interstitial pH at rest and during exercise in vivo, 2) systematically explore the effects of different ingestion regimes of 300 mg.kg-1 sodium citrate on blood and urine pH at rest, and 3) to combine the new interstitial pH technique with the findings of the second investigation in an attempt to provide a greater understanding of H+ movement between the extracellular compartments. The purpose of the first study was to develop a method for the continuous measurement of interstitial pH in vastus lateralis was successfully developed using microdialysis and 2,7-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF). To avoid the presence of an artificial alkalosis during exercise, it was necessary to add 25 mM HCO3- to the perfusate. The outlet of the probe was cut less than 10 mm from the skin and connected to a stainless steel tube completing the circuit to a microflow-through cuvette (8 fÝl) within a fluorescence spectrophotometer. This prevented the loss of carbon dioxide from the dialysate and any subsequent pH artefact. Interstitial pH was collected from six subjects before, during and after five minutes of knee-extensor exercise at three intensities 30, 50, and 70 W. Mean,,bSEM interstitial pH at rest was 7.38,,b0.02. Exercise reduced interstitial pH in an almost linear fashion. The nadir value for interstitial pH at 30, 50 and 70 W exercise was 7.27, 7.16 and 7.04, respectively. The lowest pH was obtained 1 min after exercise, irrespective of workload, after which the interstitial pH recovered in a nearly exponential manner. The mean half time of interstitial recovery was 5.2 min. The changes in interstitial pH exceeded the changes in venous blood pH. This study demonstrated that interstitial pH can be measured using microdialysis and that it is continuously decreased during muscle activity. The purpose of the second study was to establish an optimal ingestion regime for the ingestion of 300 mg.kg-1 of sodium citrate and maximise the alkalotic effect while minimising any side effects. Increasing the effectiveness of alkali ingestion may lead to further increases in muscle performance. Ingesting 300 mg.kg-1 sodium citrate at a rate of 300 mg.min-1 was identified as the optimal ingestion regime to maximise alkalosis at rest, which occurred 3.5 h post-ingestion. This was determined by monitoring eight human subjects ingesting 300 mg.kg-1 sodium citrate at five different rates, control (no ingestant), bolus, 300, 600 and 900 mg.kg.min-1 on five days separated by at least 48 hours. Sodium citrate was ingested in capsule form with water ad libitum, with the exception of bolus, which was combined with 400 ml less than 25 percent orange juice and consumed in less than 1 min. Arterialised blood (mean 71.3,,b3.5 mmHg) acid-base and electrolyte status was assessed via the withdrawal of ~5 ml of blood every 30 min across an eight hour duration, placed on ice and analysed within five minutes. No alkalotic difference was found between ingestion rates (mean 7.445,,b0.004, 7.438,,b0.004 and 7.442,,b0.004 for 300, 600 and 900 mg.min-1, respectively). All experimental ingestion regimes were associated with elevations in [HCO3-] (29.6, 29.7, 29.8, 29.9 and 26.3 mmol.l-1 for bolus, 300, 600, 900 and control, respectively). The 300 ingestion regime had the greatest impact on [H+], a 0.66 meq.l-1,,e10-8 change. Bolus ingestion (3.93,,b0.08 mmol.l-1) of sodium citrate had no effect on control (4.06,,b0.08 mmol.l-1) blood [K+], however, 300 mg.min-1 decreased blood [K+] (p less than 0.05). There was no effect of sodium citrate on blood [Cl-], but after 2.5 h blood [Cl-] was lower than pre-ingestion values (p less than0.05). All ingestion rates of sodium citrate increased (p less than 0.05) urine pH above control. This is the first study to investigate the effect of varying ingestion rates on acid-base status at rest in humans. The results suggest that ingesting sodium citrate in small doses in quick succession induce a greater blood alkalosis than the commonly practised bolus protocol. Using the interstitial pH technique described above and the optimal ingestion regime (300 mg.min-1) identified above, the final experiment was designed to assess the influence of sodium citrate ingestion on interstitial pH at both rest and during exercise. Five subjects ingested 300 mg.kg-1 sodium citrate at 300 mg.min-1 again in capsule form with water ad libitum. Prior to ingestion, each subject had a cannula placed into their cephalic vein and one microdialysis probe (CMA-60) inserted into their left thigh, orientated along the fibres of vastus lateralus. This probe was used for the measurement of pH as described above. At the end of this period, an exercise protocol required five subjects to perform light exercise (10 W) for 10 min, before starting an intense exercise period (~90-95% leg VO2peak) to exhaustion followed by a 15 min recovery period. Dialysate and blood samples were collected across all periods. Mean,,bSEM interstitial pH for placebo and alkalosis were 7.38,,b0.12 and 7.24,,b0.16, respectively. Sodium citrate ingestion was not associated with an interstitial alkalosis. An exercise induced acidosis was observed in the interstitium during placebo but not during alkalosis (p less than 0.05). Mean,,bSEM venous pH were 7.362,,b0.003 and 7.398,,b0.003 for placebo and alkalosis, respectively. Sodium citrate ingestion was not associated with a venous alkalosis. Sodium citrate ingestion was associated with an increase in mean,,bSEM venous [HCO3-] (placebo 25.5,,b0.2, alkalosis 28.1,,b0.2). This increase in the blood bicarbonate buffer system was not associated with an increase in time to exhaustion (placebo 352,,b71, alkalosis 415,,b171). This was the first study to investigate the effects of sodium citrate ingestion on interstitial pH. The results of this study demonstrated that an interstitial alkalosis does not ensue after alkali ingestion, however, it was associated with the lack of an exercise induced acidosis suggesting an improved pH regulation during exercise.

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Bhowmik, Swati. "Development of analytical methods for the gas chromatographic determination of 1,2-epoxy-3-butene, 1,2:3,4-diepoxybutane, 3-butene-1,2-diol, 3,4-epoxybutane-1,2-diol and crotonaldehyde from perfusate samples of 1,3-butadiene exposed isolated mouse and rat livers." [S.l. : s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=967431743.

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Weinreb, Ross H. "Fabrication of a tissue- engineered perfusable skin flap." Thesis, 2016. https://hdl.handle.net/2144/16753.

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To date, the reconstructive approach addressing chronic non-healing wounds, deep tissue damage, and severe wound defects relies upon avascular dermal grafts and autologous flap techniques. Such flaps are limited by donor site availability and morbidity, while current dermal grafts rely upon host cellular invasion for neovascularization and incorporation. These products fail to include an inherent vascular network and the supporting cells necessary to ensure adequate incorporation and graft survival beyond the most optimal wound beds. Herein, we fabricate a pre-vascularized full-thickness cellularized skin equivalent containing a three-dimensional vascularized network of interconnected macro and microchannels lined with vascular cells, within a collagen neodermis populated with fibroblasts, and an epidermis comprised of human keratinocytes capable of providing whole tissue perfusion. Previously, our lab has employed a sacrificial microfiber technique to develop tissue-engineered scaffolds with an inherent hierarchical network of microvessels, which recapitulates the organization of an arteriole, venule, and capillary bed. Utilizing a type-I collagen hydrogel matrix, vascular cells were seeded within pre-fabricated channels and allowed to proliferate to generate an endothelialized microvasculature. These collagen scaffolds were subsequently anastomosed into rat models to demonstrate the clinical feasibility of such approach. The present study aims to more closely recapitulate the in vivo structure of human skin via the incorporation of vital epidermal and dermal components of native skin into a biocompatible construct containing a complex hierarchical vasculature, which may be anastomosed using standard microsurgical techniques and immediately perfused. Pluronic F127 was used as the sacrificial material: 1.5 mm diameter “U” shaped macrofibers and 100-500 µm-interwoven microfibers were heat extruded and then embedded within type-I collagen into which Cyan Fluorescent Protein (CFP)-tagged human placental pericytes and human foreskin fibroblasts (HFF1) had been encapsulated. Following pluronic sacrifice, resultant channels were intraluminally seeded with Red Fluorescent Protein (RFP)-tagged human aortic smooth muscle cells, Green Fluorescent Protein (GFP)-tagged human umbilical vein endothelial cells, and topically seeded with human epidermal keratinocytes (HEK). Construct microstructure was analyzed using multiphoton microscopy (MPM) after 7, 14 and 28 days of culture. Additionally, after 14 and 28 days of culture, endothelial cells were extracted from the construct using collagenase digestion and Real Time (RT)-qPCR performed to analyze expression of markers of angiogenesis and maturation of the vascular network. MPM demonstrated a hierarchical vascular network containing macro and microvessels lined by endothelial and smooth muscle cells, supported by perivascular pericytes, all in appropriate microanatomic arrangement. Neodermal HFF1 proliferated throughout the observation period and the HEK neoepidermis developed into a stratified epidermis along the superior aspect of the construct. Angiogenic sprouting from the nascent vascular network into neovessel like structures was noted. RT- qPCR revealed relative expression of Jagged1, Dll4, Ve-Cadherin, and CD31. We have successfully fabricated a novel tissue-engineered pre-vascularized full thickness skin flap, which recapitulates the inherent hierarchical vasculature found within human skin and is suitable for in vivo perfusion. We provide the platform for an on- demand, geometrically tunable tissue engineered skin equivalent with an anastomosable vascular network. This tissue-engineered skin flap holds the potential to transform reconstructive surgical practice by eliminating the consequences of donor site morbidity, and enabling rationally designed, patient-specific flaps for each unique wound environment and anatomic location.
2017-06-16T00:00:00Z

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Leng, Lian. "Flow-based Organization of Perfusable Soft Material in Three Dimensions." Thesis, 2010. http://hdl.handle.net/1807/24259.

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This thesis presents a microfluidic strategy for the in-flow definition of a 3D soft material with a tunable and perfusable microstructure. The strategy was enabled by a microfluidic device containing up to fifteen layers that were individually patterned in polydimethylsiloxane (PDMS). Each layer contained an array of ten to thirty equidistantly spaced microchannels. Two miscible fluids (aqueous solutions of alginate and CaCl2) were used as working fluids and were introduced into the device via separate inlets and distributed on chip to form a complex fluid at the exit. The fluid microstructure was tuned by altering the flow rates of the working fluids. Upon solidification of alginate in the presence of calcium chloride, the created microstructure was retained and a soft material with a tunable microstructure was formed. The produced material was subsequently perfused using the same microfluidic architecture. The demonstrated strategy potentially offers applications in materials science and regenerative medicine.

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Buchteile zum Thema "Perfusable"

Tobe, Yusuke, Katsuhisa Sakaguchi, Jun Homma, et al. "Reconstruction of a Vascular Bed with Perfusable Blood Vessels Using a Decellularized Porcine Small Intestine for Clinical Application." In IFMBE Proceedings. Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-66169-4_35.

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Gronow, Gemot, and Herbert Kossmann. "Perfusate Oxygenation and Renal Function in the Isolated Rat Kidney." In Advances in Experimental Medicine and Biology. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4684-3291-6_68.

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Miyajima, Masakazu, Kazuaki Shimoji, Misuya Watanabe, Madoka Nakajima, Ikuko Ogino, and Hajime Arai. "Role of Artificial Cerebrospinal Fluid as Perfusate in Neuroendoscopic Surgery: A Basic Investigation." In Acta Neurochirurgica Supplementum. Springer Vienna, 2011. http://dx.doi.org/10.1007/978-3-7091-0923-6_21.

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Ogihara, Tohru, Therese Dupin, Haruyuki Nakane, et al. "Metabolism of Bradykinin in Isolated Perfused Rat Kidney Measurement of Kininase Activity in Perfusate and Urine." In Kinins IV. Springer US, 1986. http://dx.doi.org/10.1007/978-1-4684-5143-6_50.

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Funahara, Yoshinori, Michiko Miki, Mari Hirata, Koji Ogawa, and Hiromichi Kitaguchi. "Increase in Factor VIII Clotting Activity in the Perfusate of Isolated Dog Hind Leg and Heart by Components of Kallikrein-Kinin System." In Kinins IV. Springer US, 1986. http://dx.doi.org/10.1007/978-1-4757-0154-8_10.

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Lange, R., J. Erhard, U. Rauen, A. Hellinger, H. de Groot, and F. W. Eigler. "Injury to hepatocytes and non-parenchymal cells during the preservation of human livers with UW or HTK solution: a determination of hepatocellular enzymes in the effluent perfusate for preoperative evaluation of the transplant quality." In Transplant International. Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-662-00818-8_113.

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Phiroz, D. "Cardiopulmonary bypass perfusate." In Minimized Cardiopulmonary Bypass Techniques and Technologies. Elsevier, 2012. http://dx.doi.org/10.1533/9780857096029.2.133.

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Wilson, Colin. "Perfusate development for the NHBD." In Organ Donation and Transplantation after Cardiac Death. Oxford University Press, 2009. http://dx.doi.org/10.1093/med/9780199217335.003.0005.

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"ACUTE CHANGES IN PERFUSATE CALCIUM AND PHOSPHORUS DO NOT AFFECT 1-α-HYDROXYLASE ACTIVITY IN THE ISOLATED PERFUSED RAT KIDNEY." In Vitamin D. De Gruyter, 1988. http://dx.doi.org/10.1515/9783110846713.187.

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Konferenzberichte zum Thema "Perfusable"

Cook, Colin A., Yang Liu, Jianming Lu, Nanhai Chen, Yuman Fong, and Yu-Chong Tai. "Gas perfusable microfabricated membranes for high-density cell culture." In 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems (MEMS). IEEE, 2017. http://dx.doi.org/10.1109/memsys.2017.7863445.

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Mori, Nobuhito, Yuya Morimoto, and Shoji Takeuchi. "Skin-equivalent integrated with perfusable channels on curved surface." In 2015 28th IEEE International Conference on Micro Electro Mechanical Systems (MEMS). IEEE, 2015. http://dx.doi.org/10.1109/memsys.2015.7050961.

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Suzuki, Ryosuke, Yuya Morimoto, Ai Shima, and Shoji Takeuchi. "Stretchable and Perfusable Microfluidic Device for Cell Barrier Model." In 2020 IEEE 33rd International Conference on Micro Electro Mechanical Systems (MEMS). IEEE, 2020. http://dx.doi.org/10.1109/mems46641.2020.9056239.

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Sawayama, Jun, Fumisato Ozawa, and Shoji Takeuchi. "Continuous Glucose Monitoring of 3D Tissue Using a Perfusable Device." In 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems (MEMS). IEEE, 2019. http://dx.doi.org/10.1109/memsys.2019.8870795.

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Osaki, Tatsuya, Takahiro Kakegawa, Naoto Mochizuki, and Junji Fukuda. "Fabrication of perfusable vasculatures by using micromolding and electrochemical cell transfer." In 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). IEEE, 2013. http://dx.doi.org/10.1109/embc.2013.6611082.

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Ishii, Yasuaki, Yuya Morimoto, Ai Shima, and Shoji Takeuchi. "Formation of Micro-Size Perfusable Channels in mm-Thick Muscle Tissue." In 2020 IEEE 33rd International Conference on Micro Electro Mechanical Systems (MEMS). IEEE, 2020. http://dx.doi.org/10.1109/mems46641.2020.9056142.

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Nagata, Shogo, Yuya Moromoto, and Shoji Takeuchi. "Reconstruction of vascular niche of neural stem cells using perfusable microfluidic device." In 2018 IEEE Micro Electro Mechanical Systems (MEMS). IEEE, 2018. http://dx.doi.org/10.1109/memsys.2018.8346580.

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Nam, Eunryel, and Shoji Takeuchi. "Volatile odorant detection by corneal epithelial cells using a perfusable fluidic chamber." In 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems (MEMS). IEEE, 2017. http://dx.doi.org/10.1109/memsys.2017.7863429.

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Bircsak, K. M., V. van Duinen, S. J. Trietsch, et al. "Abstract 2051: Perfusable 3D angiogenesis in a high-throughput microfluidic culture platform." In Proceedings: AACR Annual Meeting 2018; April 14-18, 2018; Chicago, IL. American Association for Cancer Research, 2018. http://dx.doi.org/10.1158/1538-7445.am2018-2051.

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Cui, Juan, Huaping Wang, Qing Shi, et al. "Microrobotic assembly of shape-controllable microstructures to perfusable 3D cell-laden microtissues." In 2017 IEEE 7th Annual International Conference on CYBER Technology in Automation, Control, and Intelligent Systems (CYBER). IEEE, 2017. http://dx.doi.org/10.1109/cyber.2017.8446611.

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