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1

Brahmanta, Arya, Sutjipto Sutjipto, and Ida Bagus Narmada. "Histological changes during orthodontic tooth movement due to hyperbaric oxygen therapy." Dental Journal (Majalah Kedokteran Gigi) 49, no. 2 (February 14, 2017): 63. http://dx.doi.org/10.20473/j.djmkg.v49.i2.p63-66.

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Background: Mechanical force of orthodontics causes changes in periodontal ligament vascularization and blood flow, resulting in biochemical and cellular changes as well as changes in the contour of the alveolar bone and in the thickness of the periodontal ligaments. Hyperbaric oxygen (HBO) therapy is one of many solutions stimulating the growth of new blood vessels and increasing tissue oxygenation. Thus, HBO plays a role in recovery of periodontal ligament and osteoblasts. Purpose: This study aimed to determine the effects of HBO therapy for seven days on periodontal ligament size and osteob
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Pinheiro, Gabriela Veloso Vieira da Silva, Robinson Sabino-Silva, Melissa Rodrigues de Araujo, Shaiene Patrícia Gomes, Stephanie Wutke Oliveira, Emília Maria Gomes Aguiar, Léia Cardoso-Sousa, Carla Castiglia Gonzaga, and Marcela Claudino. "Experimental Acute Sepsis Reduced Number of Osteocalcin Immunolabeled Cells in Periodontal Ligament." Brazilian Dental Journal 31, no. 2 (April 2020): 143–51. http://dx.doi.org/10.1590/0103-6440202003024.

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Abstract The aim of this study was to evaluate the effect of acute sepsis in the periodontal ligament, alveolar and furcation bone in absence of periodontitis induction through histological and immunohistochemical analyses. A septic rat model was established by cecal ligation and puncture (CLP). Twelve rats were randomly divided into CLP (n=6) and Sham (n=6) groups. The animals were euthanized at 24 h and hemimandibles were submitted to histomorfometric (bone matrix, collagenous fibers, fibroblasts, osteocytes, inflammatory cells, and blood vessels) and immunohistochemical (BMP-2/4, RANKL and
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Khertek, M. V., and S. V. Logvinov. "Morfoquantitative and ultrastructural analysis of blood and lymph vessels in the first molars and premolars periodontal ligament." Bulletin of Siberian Medicine 10, no. 6 (December 28, 2011): 57–60. http://dx.doi.org/10.20538/1682-0363-2011-6-57-60.

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Morfoquantitative and ultrastructural analysis of the specific volumes of blood and lymph vessels in the periodontal first molars and premolars with different surfaces (medial, distal, buccal, and palatal) and at different levels of the roots are studied. It was noted that at physiological pressure on the molars and premolars, the specific volume of the investigated values are different. Throughout the root of the observed two zones of compression and expansion of periodontal. Depending on these areas of blood and lymphatic vessels was significantly different.
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Hasegawa, Tomoka, Yukina Miyamoto-Takasaki, Miki Abe, Zixuan Qiu, Tomomaya Yamamoto, Yimin, Taiji Yoshida, et al. "Histochemical examination on principal collagen fibers in periodontal ligaments of ascorbic acid-deficient ODS-od/od rats." Microscopy 68, no. 5 (July 3, 2019): 349–58. http://dx.doi.org/10.1093/jmicro/dfz021.

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Abstract In this study, we aimed to clarify the role of ascorbic acid in collagen synthesis in periodontal ligaments using osteogenic disorder Shionogi (ODS)/ShiJcl-od/od rats lacking L-gulonolactone oxidase. These rats cannot synthesize ascorbic acid in vivo. Eight-week-old ODS/ShiJcl-od/od male rats were administered ascorbic acid solution at a concentration of 200 mg/dL (control group, n = 6) or ascorbic acid solution at concentration of 0.3 mg/dL (insufficient group, n = 12). Six rats of the insufficient group were then given with ascorbic acid solution at concentration of 200 mg/dL for ad
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Al-Maula, Bushra Habeeb, Zena Jehad Wally, Mohanad Jameel Najm Al-Magsoosi, Rasha Hatem Dosh, Ruba M. Mustafa, Suhad Jabbar Hamed Al-Nasrawi, Abdullatif Alfutimie, and Julfikar Haider. "Studying Effects of Calcium Oxide Nanoparticles on Dentinogenesis in Male Wistar Rats." International Journal of Dentistry 2021 (July 24, 2021): 1–9. http://dx.doi.org/10.1155/2021/9983538.

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This study aimed to evaluate potential impacts of calcium oxide nanoparticles (CaO-NPs) at different dosages on predentin thickness, number of blood vessels, periodontal ligament thickness, and blood glucose level of Wistar rats. Twelve rats were randomly gathered into four groups, untreated (control) and CaO-NP-treated groups at three concentrations (25, 50, and 100 mg/kg of the body weight) over a period of 60 days. Histological investigation was performed on twenty-four lower incisor teeth extracted from all the tested groups under a light microscope, and an automatic Fujifilm was used to m
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Karakida, Lilian Mary, Cristiano Miranda de Araujo, Aline Cristina Batista Rodrigues Johann, Elisa Souza Camargo, Orlando Motohiro Tanaka, and Odilon Guariza Guariza Filho. "Interaction of Anabolic Androgenic Steroids and Induced Tooth Movement in Rats." Brazilian Dental Journal 28, no. 4 (August 2017): 504–10. http://dx.doi.org/10.1590/0103-6440201601119.

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Abstract This study evaluated the interaction between tooth movement and two anabolic androgenic steroids (AAS), Deposteron® and Nebido®. One hundred Wistar rats were divided into 3 groups: control (C) n=30, Nebido experimental (N) n=35 and Deposteron experimental (D) n=35. The control group was subdivided into 6 subgroups: 1, 2, 3, 5, 7 and 14. The experimental groups were subdivided into 7 subgroups: 0, 1, 2, 3, 5, 7 and 14, which corresponded to the day of animal’s euthanasia after applying orthodontic force. Orthodontic devices were used to induce tooth movement using 50 cN of reciprocal f
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Zanoni, Jacqueline N., Nathalia M. Lucas, Aline R. Trevizan, and Ivan D. S. Souza. "Histological evaluation of the periodontal ligament from aged wistar rats supplemented with ascorbic acid." Anais da Academia Brasileira de Ciências 85, no. 1 (March 1, 2013): 327–35. http://dx.doi.org/10.1590/s0001-37652013005000003.

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Ascorbic acid (AA) is able to neutralize reactive oxygen species and is essential for collagen synthesis. In aging process oxidative stress is elevated. This study aims to investigate the effects of AA supplementation on the periodontal ligament (PL) of rats during aging. Twenty five rats were used and divided into groups: J90 (90-day-old control), E345 (345-day-old control), E428 (428-day-old control), EA345 (345-day-old supplemented with AA from 90-day-old on) and EA428 (428-day-old supplemented with AA from 90-day-old on). We analyzed the thickness, density of fibroblasts and blood vessels
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Imber, Jean-Claude, Andrea Roccuzzo, Alexandra Stähli, Nikola Saulacic, James Deschner, Anton Sculean, and Dieter Daniel Bosshardt. "Immunohistochemical Evaluation of Periodontal Regeneration Using a Porous Collagen Scaffold." International Journal of Molecular Sciences 22, no. 20 (October 9, 2021): 10915. http://dx.doi.org/10.3390/ijms222010915.

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(1) Aim: To immunohistochemically evaluate the effect of a volume-stable collagen scaffold (VCMX) on periodontal regeneration. (2) Methods: In eight beagle dogs, acute two-wall intrabony defects were treated with open flap debridement either with VCMX (test) or without (control). After 12 weeks, eight defects out of four animals were processed for paraffin histology and immunohistochemistry. (3) Results: All defects (four test + four control) revealed periodontal regeneration with cementum and bone formation. VCMX remnants were integrated in bone, periodontal ligament (PDL), and cementum. No d
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Imamura, Kentaro, Yusuke Hamada, Wataru Yoshida, Tasuku Murakami, Saki Nakane-Koyachi, Kouki Yoshikawa, and Atsushi Saito. "Investigating the Effects of Dehydrated Human Amnion-Chorion Membrane on Periodontal Healing." Biomolecules 12, no. 6 (June 20, 2022): 857. http://dx.doi.org/10.3390/biom12060857.

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Each growth factor (GF) has different effects and targets, and plays a critical role in periodontal healing. Dehydrated human amnion-chorion membrane (dHACM) contains various GFs and has been used to enhance wound healing. The purpose of this study was to evaluate the effects of dHACM on periodontal healing, using in vitro and in vivo experimental approaches. Standardized periodontal defects were created in rats. The defects were randomly divided into three groups: Unfilled, filled with hydroxypropyl cellulose (HPC), and dHACM+HPC. At 2 and 4 weeks postoperatively, periodontal healing was anal
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Parlange, L. M., and M. R. Sims. "A T.E.M. stereological analysis of blood vessels and nerves in marmoset periodontal ligament following endodontics and magnetic incisor extrusion." European Journal of Orthodontics 15, no. 1 (February 1, 1993): 33–44. http://dx.doi.org/10.1093/ejo/15.1.33.

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11

Freezer, S. R., and M. R. Sims. "A transmission electron-microscope stereological study of the blood vessels, oxytalan fibres and nerves of mouse-molar periodontal ligament." Archives of Oral Biology 32, no. 6 (1987): 407–12. http://dx.doi.org/10.1016/0003-9969(87)90075-6.

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12

Schröder, Agnes, Leonie Barschkies, Jonathan Jantsch, Peter Proff, Lina Gölz, James Deschner, and Christian Kirschneck. "Role of Oxygen Supply in Macrophages in a Model of Simulated Orthodontic Tooth Movement." Mediators of Inflammation 2020 (July 29, 2020): 1–11. http://dx.doi.org/10.1155/2020/5802435.

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Apart from periodontal ligament fibroblasts, immune cells like macrophages also play an important mediating role in orthodontic tooth movement (OTM). Upon orthodontic force application to malpositioned teeth, macrophages in the periodontal ligament get exposed to both mechanical strain and hypoxic conditions (via a compression of blood vessels). In this study, we assessed the relative impact of orthodontically induced mechanical strain and hypoxic conditions on macrophages for the mediation and regulation of OTM. Macrophages were stimulated with physiological orthodontic compressive forces of
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Niklas, A., P. Proff, M. Gosau, and P. Römer. "The Role of Hypoxia in Orthodontic Tooth Movement." International Journal of Dentistry 2013 (2013): 1–7. http://dx.doi.org/10.1155/2013/841840.

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Orthodontic forces are known to have various effects on the alveolar process, such as cell deformation, inflammation, and circulatory disturbances. Each of these conditions affecting cell differentiation, cell repair, and cell migration, is driven by numerous molecular and inflammatory mediators. As a result, bone remodeling is induced, facilitating orthodontic tooth movement. However, orthodontic forces not only have cellular effects but also induce vascular changes. Orthodontic forces are known to occlude periodontal ligament vessels on the pressure side of the dental root, decreasing the bl
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14

Butera, Andrea, Carolina Maiorani, Annalaura Morandini, Manuela Simonini, Arianna Colnaghi, Stefania Morittu, Stefania Barbieri, et al. "Assessment of Oral Microbiome Changes in Healthy and COVID-19-Affected Pregnant Women: A Narrative Review." Microorganisms 9, no. 11 (November 19, 2021): 2385. http://dx.doi.org/10.3390/microorganisms9112385.

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During pregnancy, there are several metabolic changes and an alteration in the composition of microorganisms that inhabit the oral cavity, with an increase in pathogenic bacteria that promote the onset of gingival diseases. This review is based on research in reference to the PICO model (Problem/Intervention/Comparison/Outcome), related to changes in the oral microbiome of pregnant women and possible oral consequences in patients with COVID-19. The results showed a growth of some pathogenic bacteria in pregnant women, including Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum,
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Balic, Anamaria. "Biology Explaining Tooth Repair and Regeneration: A Mini-Review." Gerontology 64, no. 4 (2018): 382–88. http://dx.doi.org/10.1159/000486592.

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The tooth is an intricate composition of precisely patterned, mineralized matrices and soft tissues. Mineralized tissues include enamel (produced by the epithelial cells called ameloblasts), dentin and cementum (produced by mesenchymal cells called odontoblasts and cementoblasts, respectively), and soft tissues, which include the dental pulp and the periodontal ligament along with the invading nerves and blood vessels. It was perceived for a very long time that teeth primarily serve an esthetical function. In recent years, however, the role of healthy teeth, as well as the impact of oral healt
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16

Blaushild, N., Y. Michaeli, and S. Steigman. "Histomorphometric Study of the Periodontal Vasculature of the Rat Incisor." Journal of Dental Research 71, no. 12 (December 1992): 1908–12. http://dx.doi.org/10.1177/00220345920710121001.

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This study assessed quantitatively the vascular system in the cementum-related periodontal ligament (PDL) along the rat incisor. The lower left incisors of six rats (± 200 g) were subjected to routine histological procedures and cross-sectioned serially (2 μm), and the distance between each section and the apex was computed. The PDL of five sections at different levels along the tooth was divided into mesial, lingual, and lateral parts. The number and area of small and terminal arterioles, capillaries (C), sinusoids (S), post-capillary venules (PCV), and connecting venules, as well as the area
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17

Usumi-Fujita, Risa, Jun Hosomichi, Noriaki Ono, Naoki Shibutani, Sawa Kaneko, Yasuhiro Shimizu, and Takashi Ono. "Occlusal hypofunction causes periodontal atrophy and VEGF/VEGFR inhibition in tooth movement." Angle Orthodontist 83, no. 1 (June 18, 2012): 48–56. http://dx.doi.org/10.2319/011712-45.1.

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Abstract Objective: To examine changes in microvasculature and the expression of vascular endothelial growth factor A (VEGF-A) and VEGF receptor 2 (VEGFR-2) in rat hypofunctional periodontal ligament (PDL) during experimental tooth movement. Materials and Methods: Twelve-week-old male Sprague-Dawley rats were divided into normal occlusion and occlusal hypofunction groups. After a 2-week bite-raising period, rat first molar was moved mesially using a 10-gf titanium-nickel alloy closed coil spring in both groups. On days 0, 1, 2, 3, and 7 after tooth movement, histologic changes were examined by
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18

Nayak, Bob N., William A. Wiltshire, Ben Ganss, Howard Tenenbaum, Christopher A. G. McCulloch, and Charles Lekic. "Healing of Periodontal Tissues Following Transplantation of Cells in a Rat Orthodontic Tooth Movement Model." Angle Orthodontist 78, no. 5 (September 1, 2008): 826–31. http://dx.doi.org/10.2319/082807-396.1.

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Abstract Objective: To determine the fate and differentiation of transplanted periodontal ligament (PL) precursor cells and mouse embryonic stem (ES) cells and their relative capacity to regenerate wounded periodontium. Materials and Methods: Orthodontic tooth movement was introduced 24 hours before transplantation of PL or ES cells, and rats were euthanized either 24 hours or 72 hours after cell transplantation. The control rats received either no tooth movement and no cell transplantation or tooth movement and no cell transplantation. Differentiation of transplanted cells was assessed from m
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Ribeiro, Jucienne Salgado, José Vinicius Bolognesi Maciel, Luégya Amorin Henriques Knop, Maria Ângela Naval Machado, Ana Maria Trindade Grégio, and Elisa Souza Camargo. "Effect of Growth Hormone in Experimental Tooth Movement." Brazilian Dental Journal 24, no. 5 (October 2013): 503–7. http://dx.doi.org/10.1590/0103-6440201302286.

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The aim of this study was to evaluate, by histological analysis, the effect of growth hormone (GH) on periodontal ligament and alveolar bone during experimental tooth movement in rats. Eighty male Wistar rats divided into control (C) and experimental (E) groups were examined after 3, 7, 14 and 21 days under controlled climate conditions. Orthodontic force (30 cN) was applied on the maxillary first molar by an orthodontic appliance. Group E received 0.1 IU/kg/day of GH and Group C received 0.5 mL/kg/day of saline. The samples were processed and evaluated under optical microscopy and polarized l
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Sasaki, T., T. Shimizu, C. Watanabe, and Y. Hiyoshi. "Cellular Roles in Physiological Root Resorption of Deciduous Teeth in the Cat." Journal of Dental Research 69, no. 1 (January 1990): 67–74. http://dx.doi.org/10.1177/00220345900690011101.

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This study has attempted to assess the importance of mesenchymal cells, fibroblasts, cementoblasts, and mononuclear phagocytes (i.e., macrophages) in physiological root resorption of feline deciduous teeth. Deciduous incisors of three- to six-month-old kittens undergoing root resorption were investigated by means of electron microscopy. In an early phase of root resorption, the resorption organ consisted of many fibroblasts and relatively few macrophages and odontoclasts, the last with a wide, clear zone and narrow, immature, ruffled border. In the active phase of root resorption, the resorpti
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Tirez, Elisabeth, and Mariano S. Pedano. "Regeneration of the Pulp Tissue: Cell Homing versus Cell Transplantation Approach: A Systematic Review." Materials 15, no. 23 (December 2, 2022): 8603. http://dx.doi.org/10.3390/ma15238603.

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Background: The main objective of this systematic review was to compare the apical healing, root maturation and histological characteristics of teeth treated with cell-based versus cell-free techniques. Methods: The methodology of this review was based on the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines. A literature search strategy was carried out on PubMed, EMBASE and the Web of Science databases. The last search was done on 1 August 2021. Articles written in languages other than English were excluded. Two researchers independently selected the studi
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Utreja, Achint, and Carla A. Evans. "Marfan Syndrome—An Orthodontic Perspective." Angle Orthodontist 79, no. 2 (March 1, 2009): 394–400. http://dx.doi.org/10.2319/112707-558.1.

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Abstract Marfan syndrome is a heritable disorder of connective tissue that can affect the heart, blood vessels, lungs, eyes, bones, and ligaments. It is characterized by tall stature, elongated extremities, scoliosis, and a protruded or caved-in breastbone. Patients typically have a long, narrow face. A high-arched palate produced by a narrow maxilla and skeletal Class II malocclusion due to mandibular retrognathia are other common features. For a patient with no family history of the disorder, at least three body systems must be affected before a diagnosis can be made. Individuals affected by
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23

Shintcovsk, Ricardo Lima, Luégya Knop, Orlando Motohiro Tanaka, and Hiroshi Maruo. "Nicotine effect on bone remodeling during orthodontic tooth movement: Histological study in rats." Dental Press Journal of Orthodontics 19, no. 2 (April 2014): 96–107. http://dx.doi.org/10.1590/2176-9451.19.2.096-107.oar.

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Introduction: Nicotine is harmful to angiogenesis, osteogenesis and synthesis of collagen. Objective: The aim of this study was to investigate the effect of nicotine on bone remodeling during orthodontic movement in rats. Methods: Eighty male Wistar rats were randomly divided into three groups: Group C (control), group CM (with orthodontic movement) and group NM (nicotine with orthodontic movement) groups. The animals comprising groups C and CM received 0.9% saline solution while group NM received nicotine solution (2 mg/kg). A nickel-titanium closed-coil spring was used to induce tooth moveme
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Rahardjo, Pambudi, Maria Lisdiana Tandjung, and Dandy Bayu Angkasa. "PENGARUH KOMBINASI TERAPI OKSIGEN HIPERBARIK DAN PROPOLIS TERHADAP DIAMETER PEMBULUH DARAH DI DAERAH TARIKAN LIGAMEN PERIODONTAL SELAMA PERGERAKAN GIGI UNTUK MENCEGAH RELAPS." DENTA 13, no. 1 (February 1, 2019). http://dx.doi.org/10.30649/denta.v13i1.183.

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<strong><em>Background</em></strong><em>: The mechanical force of orthodontic device causes occurrence of a tension and a pressure area in the periodontal ligament. In the tension area, an inflammatory response characterized by vasodilation of blood vessels will occur. The function of blood vessel is to flow blood and other nutrients to accelerate the periodontal ligament remodeling process, so that relapse can be prevented. <strong>Objective:</strong> To determine the effect of combination of propolis 3%, 5% for 14 days and HBOT 2,4 ATA 3x30 minutes w
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Chang, Pi En, Shujin Li, Hyun-Yi Kim, Dong-Joon Lee, Yoon Jeong Choi, and Han-Sung Jung. "BBS7–SHH Signaling Activity Regulates Primary Cilia for Periodontal Homeostasis." Frontiers in Cell and Developmental Biology 9 (December 7, 2021). http://dx.doi.org/10.3389/fcell.2021.796274.

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Objectives: Mechanical stimuli are essential for the maintenance of periodontal ligament (PDL) homeostasis. Although there are several studies on atrophic changes in PDL due to occlusal hypofunction, the underlying mechanism is still unknown. Here, we aimed to explore the changes of gene expression in occlusal hypofunctional PDL and elucidate the related role in maintaining the PDL homeostasis.Methods: To investigate the transcriptomic difference between control and hypofunctional PDL tissue from patients, RNA sequencing was performed on 34 human teeth. The atrophic changes in PDL were evaluat
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Faccini, Melissa, Felipe Agostini, Tassio Drieu, Francisco Ubiratan Ferreira de Campos, Aguinaldo Garcez, Glauber Fabre Carinhena, Samira Salmeron, Ana Regina Casaroto, Fabricio Pinelli Valarelli, and Karina Maria Salvatore Freitas. "Preliminary Histological Evaluation of the Application of Ozone in the First Days of Orthodontic Force Induction in Animal Model." European Journal of Dentistry, August 24, 2021. http://dx.doi.org/10.1055/s-0041-1731886.

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Abstract Objectives The aim of the study is to histologically evaluate the effect of ozone therapy on orthodontic force induction in an animal model. Materials and Methods Twenty-four Wistar rats were divided into three groups (n = 8). A NiTi coil spring was installed from the maxillary first molar to the maxillary central incisor. G1 was control and G2/G3 received 1 mL of ozonated gas at concentrations of 10 and 60 µg/mL, in the buccal mucosa above the first molar roots. The animals were euthanized 3 and 5 days after the procedure. Histological sections were obtained, longitudinally of the fi
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"Effects of simulated weightlessness on the migration of osteoblast precursor cells relative to blood vessels in rat periodontal ligament utilizing 3H-thymidine label." American Journal of Orthodontics and Dentofacial Orthopedics 100, no. 1 (July 1991): 96. http://dx.doi.org/10.1016/s0889-5406(08)80028-5.

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Ullrich, Niklas, Agnes Schröder, Jonathan Jantsch, Gerrit Spanier, Peter Proff, and Christian Kirschneck. "The role of mechanotransduction versus hypoxia during simulated orthodontic compressive strain—an in vitro study of human periodontal ligament fibroblasts." International Journal of Oral Science 11, no. 4 (November 5, 2019). http://dx.doi.org/10.1038/s41368-019-0066-x.

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Abstract During orthodontic tooth movement (OTM) mechanical forces trigger pseudo-inflammatory, osteoclastogenic and remodelling processes in the periodontal ligament (PDL) that are mediated by PDL fibroblasts via the expression of various signalling molecules. Thus far, it is unknown whether these processes are mainly induced by mechanical cellular deformation (mechanotransduction) or by concomitant hypoxic conditions via the compression of periodontal blood vessels. Human primary PDL fibroblasts were randomly seeded in conventional six-well cell culture plates with O2-impermeable polystyrene
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Shalehin, N., Y. Seki, H. Takebe, S. Fujii, T. Mizoguchi, H. Nakamura, N. Yoshiba, et al. "Gli1+-PDL Cells Contribute to Alveolar Bone Homeostasis and Regeneration." Journal of Dental Research, July 4, 2022, 002203452211069. http://dx.doi.org/10.1177/00220345221106921.

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The periodontal ligament (PDL) contains mesenchymal stem cells (MSCs) that can differentiate into osteoblasts, cementoblasts, and fibroblasts. Nevertheless, the distribution and characteristics of these cells remain uncertain. Gli1, an essential hedgehog signaling transcription factor, functions in undifferentiated cells during embryogenesis. Therefore, in the present study, the differentiation ability of Gli1+ cells was examined using Gli1-CreERT2/ROSA26-loxP-stop-loxP-tdTomato (iGli1/Tomato) mice. In 4-wk-old iGli1/Tomato mice, Gli1/Tomato+ cells were only slightly detected in the PDL, aroun
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