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1

Leavitt, Markley Carl. "Bacteriophage T5 DNA polymerase relationships of DNA polymerases." Diss., The University of Arizona, 1990. http://hdl.handle.net/10150/185335.

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T5 DNA polymerase, a highly processive single polypeptide enzyme, and PRD1 DNA polymerase, a protein-primed DNA polymerase, have been analyzed for their primary structural features. The amino acid sequence of T5 DNA polymerase reveals a high degree of homology with DNA polymerase I (Pol I) of Escherichia coli and retains many of the amino acid residues which have been implicated in the 3'-5' exonuclease and DNA polymerase activities of that enzyme. Alignment with sequences of polymerase I and T7 DNA polymerase (family A polymerases) was used to identify regions possibly involved in the high pr
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2

Roettger, Michelle P. "Insight into the Fidelity of Two X-Family Polymerases: DNA Polymerase Mu and DNA Polymerase Beta." Columbus, Ohio : Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1211074588.

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3

SALHI, SAMIA. "Dna polymerase de sulfolobus acidocaldarius : interet de l'etude des dna polymerases thermophiles." Paris 7, 1989. http://www.theses.fr/1989PA077169.

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La copie par la dna polymerase de sulfolobus acidocaldarius d'un dna simple brin uni-amorce de sequence connue a ete etudiee. Les parametres cinetiques affectant cette synthese dependent de la sequence du dna. La temperature de reaction a son importance. Les methodes employees sont la reaction polymerase en chaine, la mutagenese dirigee, le sequencage de sanger
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4

Pospiech, H. (Helmut). "The role of DNA polymerases, in particular DNA polymerase ε in DNA repair and replication". Doctoral thesis, University of Oulu, 2002. http://urn.fi/urn:isbn:9514266692.

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Abstract Analysis of the primary structure of DNA polymerase ε B subunit defined similarities to B subunits of eukaryotic DNA polymerases α, δ and ε as well as the small subunits of DNA polymerase DI of Euryarchaeota. Multiple sequence alignment of these proteins revealed the presence of 12 conserved motifs and defined a novel protein superfamily. The members of the B subunit family share a common domain architecture, suggesting a similar fold, and arguing for a conserved function among these proteins. The contribution of human DNA polymerase ε to nuclear DNA replication was studied u
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5

Mischo, Hannah. "Disengaging Polymerases : Transcriptional termination by RNA polymerase II in Saccharomyces cerevisiae and the maintenance of genome integrity." Thesis, University of Oxford, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.514968.

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6

Efthimiopoulos, Georgia. "Bypass of N2-Deoxyguanosinyl Adducts by DNA Polymerases and Kinetic Implications for Polymerase Switching." The Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1366297865.

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7

Siot, Alexandra. "Elaboration et caractérisation de polymères nanochargés." Thesis, IMT Mines Alès, 2018. http://www.theses.fr/2018EMAL0001.

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8

Linley, M. "The detection of polymerase inhibiting lesions using the polymerase arrest polymerase chain reaction assay." Thesis, Swansea University, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.637924.

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There is a constant need to determine the genotoxic potential of the agents to which the human population is exposed. The stringent testing of new products is legislatively controlled and dependent on the accumulation of sufficient scientific data to allow an analysis of the risk. It is important to predetermine any risks in the workplace prior to the presentation of disease and to provide factual public information on personal exposure e.g. the risks associated with UV light. Various experimental assays have been developed to assess the genotoxicity, mutagenicity and mcarcinogenicity of given
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9

Cramer, Janina. "Funktionelle Charakterisierung der RNA-abhängigen RNA-Polymerase des Hepatitis-C-Virus Untersuchung molekularer Mechanismen der Substratspezifität von DNA-abhängigen DNA-Polymerasen /." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=971700796.

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10

Sikorsky, Jan A. "Effect of DNA base modification on polymerase chain reaction efficiency and fidelity." Huntington, WV : [Marshall University Libraries], 2005. http://www.marshall.edu/etd/descript.asp?ref=554.

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11

Lawrence, Michael S. (Michael Scott) 1975. "[RNA polymerase ribozymes]." Thesis, Massachusetts Institute of Technology, 2005. http://hdl.handle.net/1721.1/31193.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2005.<br>Title supplied by cataloger from abstract.<br>Includes bibliographical references.<br>The RNA World is a hypothetical ancient evolutionary era during which RNA was both genome and catalyst. During that time, RNA was the only kind of enzyme yet in existence, and one of its chief duties was the replication of RNA. This scenario presupposes that among all possible RNA sequences, there exist RNA replicase ribozymes, capable of synthesizing RNA using the information in an RNA template. The goal of the present work is
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12

Armache, Karim. "Crystal structures of the complete 12-subunit RNA polymerase II and its subcomplex Rpb4/7, and modeling of RNA polymerases I and III." Diss., lmu, 2005. http://nbn-resolving.de/urn:nbn:de:bvb:19-49156.

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13

Armache, Karim-Jean. "Crystal structures of the complete 12-subunit RNA polymerase II and its subcomplex Rpb4-7, and modeling of RNA polymerases I and III." [S.l.] : [s.n.], 2005. http://edoc.ub.uni-muenchen.de/archive/00004915.

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14

Folkesson, Carl, and Ola Christensson. "Genotypning av laktostolerans (LCT-13910C>T) direkt på blod med realtids-PCR : Utvärdering av Kapa Probe Force." Thesis, Hälsohögskolan, Högskolan i Jönköping, HHJ, Avd. för naturvetenskap och biomedicin, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:hj:diva-30807.

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Hos vuxna individer förekommer två fenotyper gällande produktionen av laktas, vilka kallas laktostolerans och laktosintolerans. Vid laktosintolerans produceras otillräckliga mängder laktas vilket framkallar symptom som magsmärtor och flatulens vid intagandet av mjölkprodukter. En enbaspolymorfism (LCT-13910C&gt;T) har kopplats till laktostolerans hos nordvästeuropéer och kan genotypas med smältkurveanalys i realtids-PCR. På Laboratoriemedicin vid Länssjukhuset Ryhov används idag en metod vid genotypning av LCT-13910C&gt;T där extraktion av DNA från blod krävs innan analys. Anledningen till det
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15

Sun, Hui. "Enhancing analytical capability of piezoelectric quartz crystal and capillary electrophoresis in environmental analysis using polymerase chain reaction, molecularly imprinted polymers and nanotechnology." Click to view the E-thesis via HKUTO, 2006. http://sunzi.lib.hku.hk/hkuto/record/B36959522.

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16

Chan, Annie Yee-Man. "Interactions between the influenza virus RNA polymerase and cellular RNA polymerase II." Thesis, University of Oxford, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.670083.

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17

Neugebauer, Karla M., Inna Grishina, Anita S. Bledau, and Imke Listerman. "Extragenic Accumulation of RNA Polymerase II Enhances Transcription by RNA Polymerase III." PLOS, 2007. https://tud.qucosa.de/id/qucosa%3A27951.

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Recent genomic data indicate that RNA polymerase II (Pol II) function extends beyond conventional transcription of primarily protein-coding genes. Among the five snRNAs required for pre-mRNA splicing, only the U6 snRNA is synthesized by RNA polymerase III (Pol III). Here we address the question of how Pol II coordinates the expression of spliceosome components, including U6. We used chromatin immunoprecipitation (ChIP) and high-resolution mapping by PCR to localize both Pol II and Pol III to snRNA gene regions. We report the surprising finding that Pol II is highly concentrated ∼300 bp upstrea
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18

Neugebauer, Karla M., Inna Grishina, Anita S. Bledau, and Imke Listerman. "Extragenic Accumulation of RNA Polymerase II Enhances Transcription by RNA Polymerase III." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-184076.

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Recent genomic data indicate that RNA polymerase II (Pol II) function extends beyond conventional transcription of primarily protein-coding genes. Among the five snRNAs required for pre-mRNA splicing, only the U6 snRNA is synthesized by RNA polymerase III (Pol III). Here we address the question of how Pol II coordinates the expression of spliceosome components, including U6. We used chromatin immunoprecipitation (ChIP) and high-resolution mapping by PCR to localize both Pol II and Pol III to snRNA gene regions. We report the surprising finding that Pol II is highly concentrated ∼300 bp upstrea
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19

Lee, Sally. "Architecture of RNA polymerase II and RNA polymerase III pre-initiation transcription complexes /." Thesis, Connect to this title online; UW restricted, 1997. http://hdl.handle.net/1773/9213.

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20

Moussa, Sougueh Charmarke. "Elaboration et étude de films de polypyrroles et de polycarbazoles fonctionnalisés obtenus par oxydation électrochimique." Thesis, Bourgogne Franche-Comté, 2019. http://www.theses.fr/2019UBFCD024.

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Description détaillée du sujet : Au cours des dernières années, les électrodes recouvertes d’un film polymère déposé par voie électrochimique ont suscité un engouement croissant dans le domaine des capteurs chimiques et des biocapteurs. Un exemple d’application est celui des capteurs de pH potentiométriques qui utilisent les atomes d’azote présents dans des polymères pour détecter des variations de pH. Ces électrodes sont intéressantes en vue d’applications cliniques et biologiques comme l’analyse in vivo dans la mesure où elles sont, contrairement aux électrodes de pH traditionnelles, miniatu
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21

Chang, Ya-Wen. "The Ras/PKA pathway controls transcription of genes involved in stationary phase entry in Saccharomyces cerevisiae." Columbus, Ohio : Ohio State University, 2003. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1061214472.

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Thesis (Ph. D.)--Ohio State University, 2003.<br>Title from first page of PDF file. Document formatted into pages; contains xiii, 108 p.; also includes graphics. Includes abstract and vita. Advisor: Paul K. Herman, Dept.of Molecular, Cellular, and Developmental Biology. Includes bibliographical references (p. 96-108).
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22

Wolnik, Anna. "Association des copolymères à séquences (1->4)-a-L-guluronane en présence d’ions calcium." Thesis, Grenoble, 2014. http://www.theses.fr/2014GRENV003/document.

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Les alginates forment des gels transparents en solution aqueuse en présence de certains ions divalents. Cette propriété est principalement attribuée à la formation de zones de jonction impliquant les séquences (1-&gt;4)-a-L-guluronane de chaînes adjacentes. Des oligomères d'alginates ont été utilisés comme briques élémentaires pour la synthèse de polymères biohybrides contenant des chaines pendantes oligo-(1-&gt;4)-a-L-guluronane. La rhéologie et la diffusion de la lumière ont permis d'étudier leur gélification ionotropique. De plus, une image atomistique des associations entre chaines latéral
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23

Showalter, Alexander Keith. "KINETIC STUDIES OF TWO ERROR-PRONE DNA REPAIR ENZYMES: POSSIBLE MECHANISMS FOR VIRAL MUTAGENESIS." Connect to this title online, 2002. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1016207119.

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Thesis (Ph. D.)--Ohio State University, 2002.<br>Title from first page of PDF file. Document formatted into pages; contains xii, 97 p.; also contains graphics (some col.). Includes abstract and vita. Advisor: Ming-Daw Tsai, Dept. of Chemistry. Includes bibliographical references (p. 92-97).
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24

Burrows, Judith Ann. "DNA polymerase from Bacillus caldotenax." Thesis, Open University, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.359180.

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25

Niedbala, Angela Rochelle. "Kinetic studies of transcription initiation by wild type T7 RNA polymerase, his-tagged wild type T7 RNA polymerase and GP1-Lys222 T7 RNA polymerase." Thesis, Georgia Institute of Technology, 1995. http://hdl.handle.net/1853/27288.

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26

Sutcliffe, Josephine E. "The regulation of RNA polymerase I and RNA polymerase III transcription by the pocket proteins." Thesis, University of Glasgow, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.327577.

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27

Zhu, Weiguo. "Structure-function analysis of DNA polymerase: Purification, characterization and in vitro mutagenesis of PRD1 DNA polymerase." Diss., The University of Arizona, 1993. http://hdl.handle.net/10150/186573.

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A small lipid-containing bacteriophage PRD1 encodes a DNA polymerase that utilizes a protein primer for the initiation of DNA replication. The purification of PRD1 DNA polymerase has been hampered by the insolubility of the overexpressed enzyme in E. coli cells. A simple and rapid procedure for purification of the overexpressed PRD1 DNA polymerase has been developed. This method is based on guanidine hydrochloride denaturation and renaturation of the insoluble PRD1 DNA polymerase overexpressed in E. coli containing recombinant plasmid pEJG. The purified DNA polymerase was extensively character
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28

Swiatecka-Hagenbruch, Monika. "Phagenähnliche RNA-Polymerasen." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2009. http://dx.doi.org/10.18452/15924.

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Chloroplasten höherer Pflanzen haben kleine Genome. Trotzdem ist ihre Transkriptionsmaschinerie sehr komplex. Plastidäre Gene werden von plastidenkodierten (PEP) und kernkodierten RNA-Polymerasen (NEP) transkribiert. In der vorliegenden Arbeit wurden Promotoren plastidärer Gene und Operons von Arabidopsis thaliana charakterisiert. Zur Unterscheidung zwischen NEP- und PEP-Promotoren wurden erstmals spectinomycinbehandelte, chlorophylldefiziente Arabidopsis-Pflanzen mit fehlender PEP-Aktivität verwendet. Obwohl für einige Gene auch einzelne Promotoren lokalisiert wurden, wird die Transkription d
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29

Chadima, David. "Technologie emulzní polymerace." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2016. http://www.nusl.cz/ntk/nusl-240786.

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The diploma thesis deals with the influence of technological parameters on the emulsion copolymerization of methyl methacrylate with n butyl acrylate. The theoretical part contains knowledge in the field of influences of proces tempereture, concentration of initiator, concentration of emulsifier, stirring rate and dose rate on emulsion polymerization. In the experimental part was observed effect of the concentration of the ionic emulsifier, nonionic emulsifier, furthemore was observed effect of stirring speed and the concentration of initiator K2S2O8 on conversion of copolymerization. During a
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30

Kuhn, Claus-Dieter. "Functional Architecture of RNA polymerase I." Diss., lmu, 2008. http://nbn-resolving.de/urn:nbn:de:bvb:19-81136.

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31

Buttner, M. "RNA polymerase - DNA interactions in Streptomyces." Thesis, University of Bristol, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.354445.

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32

Patel, Premal Harshad. "Evolution of DNA polymerase active site /." Thesis, Connect to this title online; UW restricted, 2001. http://hdl.handle.net/1773/6361.

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33

Ozdemir, Ahmet Yunus. "BIOCHEMICAL STUDIES OF DNA POLYMERASE THETA." Diss., Temple University Libraries, 2019. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/560412.

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Biomedical Sciences<br>Ph.D.<br>POLQ is a unique multifunctional replication and repair gene that encodes a multidomain protein with a N-terminal superfamily 2 helicase and a C-terminal A-family polymerase. Although the function of the polymerase domain has been investigated, little is understood regarding the helicase domain. Multiple studies have reported that polymerase θ-helicase (Polθ-helicase) is unable to unwind DNA. However, it exhibits ATPase activity that is stimulated by single-stranded DNA, which presents a biochemical conundrum. In contrast to previous reports, we demonstrate that
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34

Che, Austin 1979. "Fluorescence assay for polymerase arrival rates." Thesis, Massachusetts Institute of Technology, 2003. http://hdl.handle.net/1721.1/16618.

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Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, February 2004.<br>Includes bibliographical references (p. 87-100).<br>This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.<br>To engineer complex synthetic biological systems will require modular design, assembly, and characterization strategies. The RNA polymerase arrival rate (PAR) is defined to be the rate that RNA polymerases arrive at a specified location on the DNA. Designing and character
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35

Feugeas, Olivier. "Pcr (polymerase chain reaction) et vih." Lille 2, 1990. http://www.theses.fr/1990LIL2M264.

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36

Smith, Brian A. "Mechanistic insights into a reverse polymerase." The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1353507187.

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37

White, Eleanor. "Transcription termination by RNA polymerase II." Thesis, University of Oxford, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.558432.

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RNA Polymerase II (Pol I1) is responsible for the transcription of all protein-encoding genes. Pol II termination is dependent on RNA processing signals (both terminal intron splice sites, and cleavage and polyadenylation signals) as well as specific terminator elements located downstream of the poly(A) site. Detailed analysis of the human ~- globin gene terminator has shown that it contains a sequence-specific region that promotes rapid Co-Transcriptional Cleavage (CoTC) of the nascent transcript - an essential but not well understood step in the human ~-globin gene termination process. In th
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38

Jokela, M. (Maarit). "Replicative DNA polymerase associated B-subunits." Doctoral thesis, University of Oulu, 2004. http://urn.fi/urn:isbn:9514274814.

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Abstract Replicative DNA polymerases (pols) synthesize chromosomal DNA with high accuracy and speed during cell division. In eukaryotes the process involves three family B pols (α, δ, ε), whereas in Archaea, two types of pols, families B and D, are involved. In this study the B-subunits of replicative pols were analysed at the DNA, RNA and protein levels. By cloning the cDNAs for the B-subunits of human and mouse pol ε we were able to show that the encoded proteins are not only homologous to budding yeast pol ε, but also to the second largest subunit of pol α. Later studies have revealed that
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39

Tran, Tuan Anh. "Screening against the dengue virus polymerase." Thesis, Aix-Marseille, 2016. http://www.theses.fr/2016AIXM4006.

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La dengue, une des maladies les plus largement émergents actuellement, avec 390 millions d'infections chaque année (OMS), est causée par le virus de la dengue contre lequel il n’existe pas de traitements. La protéine NS5 a un rôle important dans le cycle de réplication. Cette protéine se compose d'une méthionine S-transférase d’adénosyl en N-terminal et une ARN polymérase dépendante de l'ARN (RdRp) en C-terminal. Cette NS5 RdRp peut catalyser non seulement la synthèse du brin négatif de l'ARN, utilisé comme matrice pour synthétiser l'ARN brin plus-supplémentaire, mais aussi pour la synthèse d'
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40

Ringel, Eva Rieke. "Molecular basis of RNA polymerase III transcription repression by Maf1 & Structure of human mitochondrial RNA polymerase." Diss., lmu, 2011. http://nbn-resolving.de/urn:nbn:de:bvb:19-134070.

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41

Chin, Wing-hong, and 錢永康. "Polymerase activity of chimeric polymerase : a determining factor for an influenza virus to be a pandemic strain." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hdl.handle.net/10722/193398.

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The influenza polymerase is a complex of three subunits, polymerase basic protein 2 (PB2), polymerase basic protein 1 (PB1) and polymerase acidic protein (PA). It associates with the viral RNA segment and nucleoprotein (NP) to form a viral ribonucleoprotein (vRNP) complex which is important for transcription and replication of the viral genome. Concurrently, the previous three influenza pandemics viruses contain reassorted vRNP of different origins. This leads to the aim of study to investigate the role of polymerase in the pandemic viruses. By reconstitution of vRNPs in human cells, it was de
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42

GREEN, GEORGE DAVID. "QUINODIMETHANE POLYMERS." Diss., The University of Arizona, 1986. http://hdl.handle.net/10150/183860.

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Monomers and polymers containing the quinodimethane unit were synthesized. The infrared, nuclear magnetic resonance (NMR) and ultraviolet spectra of these materials were also reported. Oxidation of the enolate dianoion of α, α'-di(carbomethoxy)-α, α'-diphenylquinodimethane (DMPQH₂) with iodine gave 7,8-di(carbomethoxy)-7,8-diphenylguinodimethane (DMPQ) in greater than 50% yield. This compound had a reduction potential of -0.85V (Ag/AgCl reference) but would not form charge transfer complexes with electron donors. Attempted polymerization reactions of DMPQ were also discussed. A series of donor
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43

Dann, A. J. "Phthalocyanine polymers." Thesis, University of Nottingham, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376167.

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44

Smith, A. E. "Functionalised polymers." Thesis, Aston University, 1988. http://publications.aston.ac.uk/9733/.

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Synthetic routes to polymers possessing functional groups were studied. Direct functionalisation of poly(vinyltoluene) by lithiation and carboxylation resulted in the expected carboxylic acid but reaction was complicated by the production of a mixture of products. Reaction occurred both at the polymer backbone and at the pendant methyl group. Reaction with ethyl formate was also difficult to control and a secondary alcohol was formed even when an excess of the carbonyl compounds was employed. Grignard formation of poly(bromostyrene) was successful but once formed, the derivative rearranged res
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45

Arndt, Joseph W. "Characterization and structural determination of metalloenzymes DNA polymerase beta, carboxypeptidase, and acetyl coenzyme-A decarbonylase/synthase /." Columbus, OH : Ohio State University, 2003. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1061312369.

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Thesis (Ph. D.)--Ohio State University, 2003.<br>Title from first page of PDF file. Document formatted into pages; contains xxii, 172 p. : ill., some col. Includes abstract and vita. Advisor: Michael K. Chan, Dept. of Chemistry. Includes bibliographical references (p. 165-172).
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46

Liu, Karen Ka Yan. "Origins of shear strength of polymers and reinforced polymers." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp02/NQ27996.pdf.

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47

Lai, Benjamin Fook Lun. "Bioactive polymers : a comparative study on the antithrombotic properties of soluble polymers and surface grafted polymers." Thesis, University of British Columbia, 2010. http://hdl.handle.net/2429/20871.

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Use of synthetic materials in medical applications is one of the most common practices in modern medicine. Yet occurrence of surface-induced thrombus formation on these materials, especially those associated with cardiovascular applications, generates a need for surface modifications. Limiting thrombus formation on a biomaterial surface represents the ultimate success for blood contacting devices. One interesting approach is to enhance fibrinolysis before the blood clot becomes stabilized. Herein, two synthetic polymers, poly-N- [(2, 2-dimethyl-1, 2-dioxolane) methyl] acrylamide (PDMDOMA)
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48

KUO, THAU-MING. "SYNTHESIS AND CHARACTERIZATION OF MONOMERS AND POLYMERS CONTAINING MULTIPLE P-ARYLENEAZO OR P-BENZOQUINODIIMINE GROUPS: CONDUCTING POLYMERS, LIQUID CRYSTAL POLYMERS, AND DIPOLAR POLYMERS." Diss., The University of Arizona, 1987. http://hdl.handle.net/10150/184215.

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Aniline Black, a polymer containing p-benzoquinodiimine groups, was synthesized chemically or electrochemically by the oxidation of aniline. The polymer salts showed the conductivity of 10⁻¹-10⁻³ ohm⁻¹cm⁻¹. Polymers containing anthroquinodiimine units were also prepared by polycondensations. The syntheses of model compounds containing p-benzoquinodiimine were attempted. Multiazobisphenol monomers were synthesized. 4,4'-(3,3'-Dimethyl-4,4'-biphenylenebisazo) bisphenol 7, 4,4'-[azobis(p-phenyleneazo)] bisphenol 8, and 4,4'-(2-methoxy-1,4-phenylenebisazo) bisphenol 10 displayed liquid crystal (l.
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49

Mäkiniemi, M. (Minna). "Human DNA polymerase ε associated proteins:identification and characterization of the B-subunit of DNA polymerase ε and TopBP1". Doctoral thesis, University of Oulu, 2001. http://urn.fi/urn:isbn:9514259688.

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Abstract DNA polymerase ε from HeLa cells has been purified as a heterodimer of a 261 kDa catalytic subunit and a tightly associated smaller polypeptide, the B-subunit. The cDNAs encoding the B-subunits of both human and mouse Pol ε were cloned and shown to encode proteins with a predicted molecular weight of 59 kDa. These subunits are 90 % identical and share 22 % identity with the 80 kDa B-subunit of Saccharomyces cerevisiae Pol ε. The gene for the human Pol ε B-subunit was localized to chromosome 14q21-q22 by fluorescence in situ hybridization. Primary structure analysis of the Pol ε B-sub
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50

Thuresson, Ann-Charlotte. "Regulation of Mammalian Poly(A) Polymerase Activity." Doctoral thesis, Uppsala University, Department of Cell and Molecular Biology, 2002. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2892.

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<p>Poly(A) polymerase (PAP) is the enzyme catalyzing the synthesis of the adenine tail to the 3’-end of mRNA. This A-tail is present on the majority of the primary RNA transcripts of protein-coding genes, and is important for mRNA stability, export to the cytoplasm and translation. Therefore, PAP is a key regulator of eukaryotic gene expression. This thesis describes the heterogeneity of PAP and the functional significance of multiple isoforms of PAP. </p><p>PAP exists in many different isoforms generated by three different mechanisms, gene duplication, alternative mRNA processing and post-tra
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