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Thematische Bibliographien / Replicating / Zeitschriftenartikel

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Zeitschriftenartikel zum Thema „Replicating“

Autor: Grafiati

Veröffentlicht am 29. Juni 2021

Zuletzt aktualisiert am 29. Juli 2025

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1

Coffman, Lucas C., Muriel Niederle, and Alistair J. Wilson. "A Proposal to Organize and Promote Replications." American Economic Review 107, no. 5 (2017): 41–45. http://dx.doi.org/10.1257/aer.p20171122.

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We make a two-pronged proposal to (i) strengthen the incentives for replication work and (ii) better organize and draw attention to the replications that are conducted. First we propose that top journals publish short “replication reports.” These reports could summarize novel work replicating an existing high-impact paper, or they could highlight a replication result embedded in a wider-scope published paper. Second, we suggest incentivizing replications with the currency of our profession: citations. Enforcing a norm of citing replication work alongside the original would provide incentives f

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Robinson, D. R., and K. Gull. "The configuration of DNA replication sites within the Trypanosoma brucei kinetoplast." Journal of Cell Biology 126, no. 3 (1994): 641–48. http://dx.doi.org/10.1083/jcb.126.3.641.

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The kinetoplast is a concatenated network of circular DNA molecules found in the mitochondrion of many trypanosomes. This mass of DNA is replicated in a discrete "S" phase in the cell cycle. We have tracked the incorporation of the thymidine analogue 5-bromodeoxyuridine into newly replicated DNA by immunofluorescence and novel immunogold labeling procedures. This has allowed the detection of particular sites of replicated DNA in the replicating and segregating kinetoplast. These studies provide a new method for observing kinetoplast DNA (kDNA) replication patterns at high resolution. The techn

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Kelly, Clint D. "Rate and success of study replication in ecology and evolution." PeerJ 7 (September 10, 2019): e7654. http://dx.doi.org/10.7717/peerj.7654.

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The recent replication crisis has caused several scientific disciplines to self-reflect on the frequency with which they replicate previously published studies and to assess their success in such endeavours. The rate of replication, however, has yet to be assessed for ecology and evolution. Here, I survey the open-access ecology and evolution literature to determine how often ecologists and evolutionary biologists replicate, or at least claim to replicate, previously published studies. I found that approximately 0.023% of ecology and evolution studies are described by their authors as replicat

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van ’t Wout, Angélique B., Hetty Blaak, Leonie J. Ran, Margreet Brouwer, Carla Kuiken, and Hanneke Schuitemaker. "Evolution of Syncytium-Inducing and Non-Syncytium-Inducing Biological Virus Clones in Relation to Replication Kinetics during the Course of Human Immunodeficiency Virus Type 1 Infection." Journal of Virology 72, no. 6 (1998): 5099–107. http://dx.doi.org/10.1128/jvi.72.6.5099-5107.1998.

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ABSTRACT To investigate the temporal relationship between human immunodeficiency virus type 1 (HIV-1) replicative capacity and syncytium-inducing (SI) phenotype, biological and genetic characteristics of longitudinally obtained virus clones from two HIV-1-infected individuals who developed SI variants were studied. In one individual, the emergence of rapidly replicating SI and non-syncytium-inducing (NSI) variants was accompanied by a loss of the slowly replicating NSI variants. In the other subject, NSI variants were always slowly replicating, while the coexisting SI variants showed an increa

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Quintini, G., K. Treuner, C. Gruss, and R. Knippers. "Role of amino-terminal histone domains in chromatin replication." Molecular and Cellular Biology 16, no. 6 (1996): 2888–97. http://dx.doi.org/10.1128/mcb.16.6.2888.

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Simian virus 40 minichromosomes were treated with trypsin to specifically remove the amino-terminal histone domains (tails). Trypsin treatment does not affect the spacing and the number of nucleosomes on minichromosomes but indices a more extended conformation, as shown by the reduced sedimentation coefficient of trypsinized minichromosomes compared with the untreated controls. Trypsinized minichromosomes replicate more efficiently than control minichromosomes in in vitro replication assays. The increased template efficiency appears to be due to higher rates of replicative fork movement. In vi

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Reggia, James A., Jason D. Lohn, and Hui-Hsien Chou. "Self-Replicating Structures: Evolution, Emergence, and Computation." Artificial Life 4, no. 3 (1998): 283–302. http://dx.doi.org/10.1162/106454698568594.

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Since von Neumann's seminal work around 1950, computer scientists and others have studied the algorithms needed to support self-replicating systems. Much of this work has focused on abstract logical machines (automata) embedded in two-dimensional cellular spaces. This research was motivated by the desire to understand the basic information-processing principles underlying self-replication, the potential long-term applications of programmable self-replicating machines, and the possibility of gaining insight into biological replication and the origins of life. We view past research as taking thr

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Avemann, K., R. Knippers, T. Koller, and J. M. Sogo. "Camptothecin, a specific inhibitor of type I DNA topoisomerase, induces DNA breakage at replication forks." Molecular and Cellular Biology 8, no. 8 (1988): 3026–34. http://dx.doi.org/10.1128/mcb.8.8.3026-3034.1988.

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The structure of replicating simian virus 40 minichromosomes, extracted from camptothecin-treated infected cells, was investigated by biochemical and electron microscopic methods. We found that camptothecin frequently induced breaks at replication forks close to the replicative growth points. Replication branches were disrupted at about equal frequencies at the leading and the lagging strand sides of the fork. Since camptothecin is known to be a specific inhibitor of type I DNA topoisomerase, we suggest that this enzyme is acting very near the replication forks. This conclusion was supported b

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Avemann, K., R. Knippers, T. Koller, and J. M. Sogo. "Camptothecin, a specific inhibitor of type I DNA topoisomerase, induces DNA breakage at replication forks." Molecular and Cellular Biology 8, no. 8 (1988): 3026–34. http://dx.doi.org/10.1128/mcb.8.8.3026.

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The structure of replicating simian virus 40 minichromosomes, extracted from camptothecin-treated infected cells, was investigated by biochemical and electron microscopic methods. We found that camptothecin frequently induced breaks at replication forks close to the replicative growth points. Replication branches were disrupted at about equal frequencies at the leading and the lagging strand sides of the fork. Since camptothecin is known to be a specific inhibitor of type I DNA topoisomerase, we suggest that this enzyme is acting very near the replication forks. This conclusion was supported b

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Walker, Richard M., Gene A. Brewer, M. Jin Lee, Nicolai Petrovsky, and Arjen van Witteloostuijn. "Best Practice Recommendations for Replicating Experiments in Public Administration." Journal of Public Administration Research and Theory 29, no. 4 (2018): 609–26. http://dx.doi.org/10.1093/jopart/muy047.

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Abstract Replication is an important mechanism through which broad lessons for theory and practice can be drawn in the applied interdisciplinary social science field of public administration. We suggest a common replication framework for public administration that is illustrated by experimental work in the field. Drawing on knowledge from other disciplines, together with our experience in replicating several experiments on topics such as decision making, organizational rules, and government–citizen relationships, we provide an overview of the replication process. We then distill this knowledge

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Jazwinski, S. M. "Participation of ATP in the binding of a yeast replicative complex to DNA." Biochemical Journal 246, no. 1 (1987): 213–19. http://dx.doi.org/10.1042/bj2460213.

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The activity that replicates yeast DNA in vitro can be isolated from cells of the budding yeast Saccharomyces in a high-Mr (approximately 2 × 10(6] form. Several lines of evidence indicate that this fraction contains a multiprotein replicative complex. A functional assay has been developed for the analysis of the interaction of the replicating activity with DNA. Binding of the activity required Mg2+, but did not require the addition of ATP or the other ribo- or deoxynucleoside triphosphates. However, the ATP analogues adenosine 5′-[gamma-thio]triphosphate and adenosine 5′-[beta gamma-imido]tri

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Hoggard, Timothy, Carolin A. Müller, Conrad A. Nieduszynski, Michael Weinreich, and Catherine A. Fox. "Sir2 mitigates an intrinsic imbalance in origin licensing efficiency between early- and late-replicating euchromatin." Proceedings of the National Academy of Sciences 117, no. 25 (2020): 14314–21. http://dx.doi.org/10.1073/pnas.2004664117.

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A eukaryotic chromosome relies on the function of multiple spatially distributed DNA replication origins for its stable inheritance. The spatial location of an origin is determined by the chromosomal position of an MCM complex, the inactive form of the DNA replicative helicase that is assembled onto DNA in G1-phase (also known as origin licensing). While the biochemistry of origin licensing is understood, the mechanisms that promote an adequate spatial distribution of MCM complexes across chromosomes are not. We have elucidated a role for the Sir2 histone deacetylase in establishing the normal

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Makel, Matthew C., Jonathan A. Plucker, and Boyd Hegarty. "Replications in Psychology Research." Perspectives on Psychological Science 7, no. 6 (2012): 537–42. http://dx.doi.org/10.1177/1745691612460688.

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Recent controversies in psychology have spurred conversations about the nature and quality of psychological research. One topic receiving substantial attention is the role of replication in psychological science. Using the complete publication history of the 100 psychology journals with the highest 5-year impact factors, the current article provides an overview of replications in psychological research since 1900. This investigation revealed that roughly 1.6% of all psychology publications used the term replication in text. A more thorough analysis of 500 randomly selected articles revealed th

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Ashari, Siti Auji. "A systematic review of the theory of mind studies and the potential for replications in linguistics." International Journal of Modern Languages and Applied Linguistics 6, no. 4 (2022): 13–37. http://dx.doi.org/10.24191/ijmal.v6i4.19808.

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The theory of mind is a psychological concept that refers to the awareness of one’s mental states and others’. In the seminal study conducted by Kidd and Castano (2013), the researchers found that reading literary fiction led to better performance in the theory of mind test relative to reading popular fiction, nonfiction, or reading nothing at all. This paper collates seven subsequent replications of the original study: five direct replications and two indirect ones using other media, namely the visual narrative of television and movies. The findings of these replications are then compared to

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Iwasaki, Hiromichi, Peng Huang, Michael J. Keating, and William Plunkett. "Differential Incorporation of Ara-C, Gemcitabine, and Fludarabine Into Replicating and Repairing DNA in Proliferating Human Leukemia Cells." Blood 90, no. 1 (1997): 270–78. http://dx.doi.org/10.1182/blood.v90.1.270.

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Abstract The major actions of nucleoside analogs such as arabinosylcytosine (ara-C) and fludarabine occurs after their incorporation into DNA, during either replication or repair synthesis. The metabolic salvage and DNA incorporation of the normal nucleoside, deoxycytidine, is functionally compartmentalized toward repair synthesis in a process regulated by ribonucleotide reductase. The aim of this study was to investigate the metabolic pathways by which nucleoside analogs that do (fludarabine, gemcitabine) or do not (ara-C) affect ribonucleotide reductase are incorporated into DNA in prolifera

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Iwasaki, Hiromichi, Peng Huang, Michael J. Keating, and William Plunkett. "Differential Incorporation of Ara-C, Gemcitabine, and Fludarabine Into Replicating and Repairing DNA in Proliferating Human Leukemia Cells." Blood 90, no. 1 (1997): 270–78. http://dx.doi.org/10.1182/blood.v90.1.270.270_270_278.

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The major actions of nucleoside analogs such as arabinosylcytosine (ara-C) and fludarabine occurs after their incorporation into DNA, during either replication or repair synthesis. The metabolic salvage and DNA incorporation of the normal nucleoside, deoxycytidine, is functionally compartmentalized toward repair synthesis in a process regulated by ribonucleotide reductase. The aim of this study was to investigate the metabolic pathways by which nucleoside analogs that do (fludarabine, gemcitabine) or do not (ara-C) affect ribonucleotide reductase are incorporated into DNA in proliferating huma

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Hasse, Steven B., and Michele P. Calos. "Replication control of autonomously replicating human sequence." Nucleic Acids Research 19, no. 18 (1991): 5053–58. http://dx.doi.org/10.1093/nar/19.18.5053.

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Pierron, Gérard, Dominick Pallotta, and Marianne Bénard. "The One-Kilobase DNA Fragment Upstream of theardC Actin Gene of Physarum polycephalum Is Both a Replicator and a Promoter." Molecular and Cellular Biology 19, no. 5 (1999): 3506–14. http://dx.doi.org/10.1128/mcb.19.5.3506.

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ABSTRACT The 1-kb DNA fragment upstream of the ardC actin gene of Physarum polycephalum promotes the transcription of a reporter gene either in a transient-plasmid assay or as an integrated copy in an ectopic position, defining this region as the transcriptional promoter of the ardC gene (PardC). Since we mapped an origin of replication activated at the onset of S phase within this same fragment, we examined the pattern of replication of a cassette containing the PardCpromoter and the hygromycin phosphotransferase gene, hph, integrated into two different chromosomal sites. In both cases, we sh

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Suzuki, Keisuke, and Takashi Ikegami. "Spatial-Pattern-Induced Evolution of a Self-Replicating Loop Network." Artificial Life 12, no. 4 (2006): 461–85. http://dx.doi.org/10.1162/artl.2006.12.4.461.

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We study a system of self-replicating loops in which interaction rules between individuals allow competition that leads to the formation of a hypercycle-like network. The main feature of the model is the multiple layers of interaction between loops, which lead to both global spatial patterns and local replication. The network of loops manifests itself as a spiral structure from which new kinds of self-replicating loops emerge at the boundaries between different species. In these regions, larger and more complex self-replicating loops live for longer periods of time, managing to self-replicate

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Beare, Brendan K. "Distributional Replication." Entropy 23, no. 8 (2021): 1063. http://dx.doi.org/10.3390/e23081063.

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A function which transforms a continuous random variable such that it has a specified distribution is called a replicating function. We suppose that functions may be assigned a price, and study an optimization problem in which the cheapest approximation to a replicating function is sought. Under suitable regularity conditions, including a bound on the entropy of the set of candidate approximations, we show that the optimal approximation comes close to achieving distributional replication, and close to achieving the minimum cost among replicating functions. We discuss the relevance of our resul

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Stauffer, André, and Moshe Sipper. "An Interactive Self-Replicator Implemented in Hardware." Artificial Life 8, no. 2 (2002): 175–83. http://dx.doi.org/10.1162/106454602320184239.

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Self-replicating loops presented to date are essentially worlds unto themselves, inaccessible to the observer once the replication process is launched. In this article we present the design of an interactive self-replicating loop of arbitrary size, wherein the user can physically control the loop's replication and induce its destruction. After introducing the BioWall, a reconfigurable electronic wall for bio-inspired applications, we describe the design of our novel loop and delineate its hardware implementation in the wall.

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Robinson, ES, PB Samollow, JL VandeBerg, and PG Johnston. "X-chromosome replication patterns in adult, newborn and prenatal opossums." Reproduction, Fertility and Development 6, no. 4 (1994): 533. http://dx.doi.org/10.1071/rd9940533.

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Somatic cells from the opossums Monodelphis domestica and Didelphis virginiana were labelled with 5-bromodeoxyuridine (BrdU), treated with colchicine, stained with acridine orange and examined using fluorescence microscopy. BrdU-incorporated metaphase spreads from females of M. domestica at developmental stages from late bilaminar blastocysts to adults showed replication asynchrony of the two (acrocentric) X chromosomes. The long arm of one X chromosome was the latest replicating region in the entire chromosome complement and is presumed to represent transcriptional inactivation and X dosage c

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Kim, Yusik, Rhonda Righter, and Ronald Wolff. "Job replication on multiserver systems." Advances in Applied Probability 41, no. 2 (2009): 546–75. http://dx.doi.org/10.1239/aap/1246886623.

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Parallel processing is a way to use resources efficiently by processing several jobs simultaneously on different servers. In a well-controlled environment where the status of the servers and the jobs are well known, everything is nearly deterministic and replicating jobs on different servers is obviously a waste of resources. However, in a poorly controlled environment where the servers are unreliable and/or their capacity is highly variable, it is desirable to design a system that is robust in the sense that it is not affected by the poorly performing servers. By replicating jobs and assignin

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Kim, Yusik, Rhonda Righter, and Ronald Wolff. "Job replication on multiserver systems." Advances in Applied Probability 41, no. 02 (2009): 546–75. http://dx.doi.org/10.1017/s0001867800003414.

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Parallel processing is a way to use resources efficiently by processing several jobs simultaneously on different servers. In a well-controlled environment where the status of the servers and the jobs are well known, everything is nearly deterministic and replicating jobs on different servers is obviously a waste of resources. However, in a poorly controlled environment where the servers are unreliable and/or their capacity is highly variable, it is desirable to design a system that is robust in the sense that it is not affected by the poorly performing servers. By replicating jobs and assignin

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Babudri, Nora, Alessandro Achilli, Chiara Martinelli, Elizabeth Moore, Hovirag Lancioni, and Yuri I. Pavlov. "The role of DNA polymerase alpha in the control of mutagenesis in Saccharomyces cerevisiae cells starved for nutrients." Ecological genetics 9, no. 1 (2011): 53–61. http://dx.doi.org/10.17816/ecogen9153-61.

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In nature, microorganisms experience numerous environmental stresses and generally grow poorly most of the time. In the last two decades it has become evident that mutations arise not only in actively dividing cells but also in nonreplicating or slowly replicating cells starved for nutrients. In yeast, precise base selection and proofreading by replicative DNA polymerases δ and ε keep starvation-associated mutagenesis (SAM) at basal levels. Less is known about the role of replicative DNA polymerase α (Pol α). Here we provide evidence that Pol α is involved in the control of SAM in yeast cells

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Boftsi, Maria, Fawn B. Whittle, Juexin Wang, et al. "The adeno-associated virus 2 genome and Rep 68/78 proteins interact with cellular sites of DNA damage." Human Molecular Genetics 31, no. 6 (2021): 985–98. http://dx.doi.org/10.1093/hmg/ddab300.

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Abstract Nuclear DNA viruses simultaneously access cellular factors that aid their life cycle while evading inhibitory factors by localizing to distinct nuclear sites. Adeno-associated viruses (AAVs), which are Dependoviruses in the family Parvovirinae, are non-enveloped icosahedral viruses, which have been developed as recombinant AAV vectors to express transgenes. AAV2 expression and replication occur in nuclear viral replication centers (VRCs), which relies on cellular replication machinery as well as coinfection by helper viruses such as adenoviruses or herpesviruses, or exogenous DNA dama

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Aguilar, Rhiannon R., and Jessica K. Tyler. "Thinking Outside the Cell: Replicating Replication In Vitro." Molecular Cell 65, no. 1 (2017): 5–7. http://dx.doi.org/10.1016/j.molcel.2016.12.019.

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Sobeck, Alexandra, Stacie Stone, Bendert deGraaf, et al. "Coordinated Chromatin-Association of Fanconi Anemia Network Proteins Requires Replication-Coupled DNA Damage Recognition." Blood 104, no. 11 (2004): 723. http://dx.doi.org/10.1182/blood.v104.11.723.723.

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Abstract Fanconi anemia (FA) is a genetic disorder characterized by hypersensitivity to DNA crosslinking agents and diverse clinical symptoms, including developmental anomalies, progressive bone marrow failure, and predisposition to leukemias and other cancers. FA is genetically heterogeneous, resulting from mutations in any of at least eleven different genes. The FA proteins function together in a pathway composed of a mulitprotein core complex that is required to trigger the DNA-damage dependent activation of the downstream FA protein, FANCD2. This activation is thought to be the key step in

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Miller, D. G., M. A. Adam, and A. D. Miller. "Gene transfer by retrovirus vectors occurs only in cells that are actively replicating at the time of infection." Molecular and Cellular Biology 10, no. 8 (1990): 4239–42. http://dx.doi.org/10.1128/mcb.10.8.4239-4242.1990.

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Previous reports have shown that retrovirus infection is inhibited in nonreplicating (stationary-phase [hereafter called stationary]) cells. Infection of stationary cells was shown to occur when the cells were allowed to replicate at times up to a week after infection, suggesting that an unintegrated retrovirus could persist in a form that was competent to integrate after release of the block to replication. However, those studies were complicated by the use of replication-competent virus, which can spread in the infected cells. We have used a replication-defective retrovirus vector to compare

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Miller, D. G., M. A. Adam, and A. D. Miller. "Gene transfer by retrovirus vectors occurs only in cells that are actively replicating at the time of infection." Molecular and Cellular Biology 10, no. 8 (1990): 4239–42. http://dx.doi.org/10.1128/mcb.10.8.4239.

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Previous reports have shown that retrovirus infection is inhibited in nonreplicating (stationary-phase [hereafter called stationary]) cells. Infection of stationary cells was shown to occur when the cells were allowed to replicate at times up to a week after infection, suggesting that an unintegrated retrovirus could persist in a form that was competent to integrate after release of the block to replication. However, those studies were complicated by the use of replication-competent virus, which can spread in the infected cells. We have used a replication-defective retrovirus vector to compare

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Enver, T., A. C. Brewer, and R. K. Patient. "Role for DNA replication in beta-globin gene activation." Molecular and Cellular Biology 8, no. 3 (1988): 1301–8. http://dx.doi.org/10.1128/mcb.8.3.1301-1308.1988.

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Transcriptional activation of the Xenopus laevis beta-globin gene requires the synergistic action of the simian virus 40 enhancer and DNA replication in DEAE-dextran-mediated HeLa cell transfections. Replication does not act through covalent modification of the template, since its requirement was not obviated by the prior replication of the transfected DNA in eucaryotic cells. Transfection of DNA over a 100-fold range demonstrates that replication does not contribute to gene activation simply increasing template copy number. Furthermore, in cotransfections of replicating and nonreplicating con

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Enver, T., A. C. Brewer, and R. K. Patient. "Role for DNA replication in beta-globin gene activation." Molecular and Cellular Biology 8, no. 3 (1988): 1301–8. http://dx.doi.org/10.1128/mcb.8.3.1301.

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Transcriptional activation of the Xenopus laevis beta-globin gene requires the synergistic action of the simian virus 40 enhancer and DNA replication in DEAE-dextran-mediated HeLa cell transfections. Replication does not act through covalent modification of the template, since its requirement was not obviated by the prior replication of the transfected DNA in eucaryotic cells. Transfection of DNA over a 100-fold range demonstrates that replication does not contribute to gene activation simply increasing template copy number. Furthermore, in cotransfections of replicating and nonreplicating con

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Lee-Chen, G. J., and M. Woodworth-Gutai. "Evolutionarily selected replication origins: functional aspects and structural organization." Molecular and Cellular Biology 6, no. 9 (1986): 3077–85. http://dx.doi.org/10.1128/mcb.6.9.3077-3085.1986.

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A selective replicative pressure occurs during the evolution of simian virus 40 variants. When the replication origin is duplicated as an inverted repeat, there is a dramatic enhancement of replication. Having regulatory sequences located between the inverted repeat of ori magnifies their enhancing effect on replication. A passage 20 variant and a passage 45 variant containing three pairs of an inverted repeat of ori replicated more efficiently than a passage 13 variant containing nine copies of ori arranged in tandem. A 69-base-pair cellular sequence inserted between inverted repeats of ori o

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Lee-Chen, G. J., and M. Woodworth-Gutai. "Evolutionarily selected replication origins: functional aspects and structural organization." Molecular and Cellular Biology 6, no. 9 (1986): 3077–85. http://dx.doi.org/10.1128/mcb.6.9.3077.

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A selective replicative pressure occurs during the evolution of simian virus 40 variants. When the replication origin is duplicated as an inverted repeat, there is a dramatic enhancement of replication. Having regulatory sequences located between the inverted repeat of ori magnifies their enhancing effect on replication. A passage 20 variant and a passage 45 variant containing three pairs of an inverted repeat of ori replicated more efficiently than a passage 13 variant containing nine copies of ori arranged in tandem. A 69-base-pair cellular sequence inserted between inverted repeats of ori o

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Taguchi, Naoko, and Shuai Li. "Replication research in contextual and individual influences in pragmatic competence: Taguchi, Xiao & Li (2016) and Bardovi-Harlig & Bastos (2011)." Language Teaching 52, no. 1 (2017): 128–40. http://dx.doi.org/10.1017/s0261444817000222.

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Recent development in L2 pragmatics research in a study abroad context has witnessed an emerging line of studies investigating the joint influences of contextual and individual learner factors on second language (L2) pragmatic development. This paper argues for the replication of two representative quantitative studies in this new research direction. Situated within the field's increasing emphasis on explaining the development of L2 pragmatic competence, the first part of this paper makes a case for the necessity of replicating quantitative studies investigating the study abroad context, highl

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Mardiah, Rika, Hanita Hanita, and Farny Sutriany Jafar. "MENINGKATKAN KEMAMPUAN MENIRU BENTUK MELALUI STRATEGI PEMBELAJARAN GRADASI DENGAN MEDIA PLASTISIN PADA KELOMPOK B DI TK TUNAS BANGSA KERTA BUANA TENGGARONG SEBERANG TAHUN AJARAN 2016/2017." Jurnal Warna : Pendidikan dan Pembelajaran Anak Usia Dini 1, no. 2 (2018): 32–44. http://dx.doi.org/10.24903/jw.v1i2.181.

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The researcher observed that students in Group B at TK Tunas Bangsa Kerta Buana Tenggarong Seberang had problems in replicating shapes. Therefore, to solve the problem, the researcher conducted a classroom action research, with purpose, to find out whether the implementation of gradation learning strategy by utilizing plasticine as the media of learning is able to enhance students’ ability in making shape replication. Moreover, the researcher involved 12 students; 7 males and 5 females, as the research subjects. To collect the data, the researcher employed observation and documentation techniq

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Gosert, Rainer, Denise Egger, Volker Lohmann, et al. "Identification of the Hepatitis C Virus RNA Replication Complex in Huh-7 Cells Harboring Subgenomic Replicons." Journal of Virology 77, no. 9 (2003): 5487–92. http://dx.doi.org/10.1128/jvi.77.9.5487-5492.2003.

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ABSTRACT Formation of a membrane-associated replication complex, composed of viral proteins, replicating RNA, and altered cellular membranes, is a characteristic feature of plus-strand RNA viruses. Here, we demonstrate the presence of a specific membrane alteration, designated the membranous web, that contains hepatitis C virus (HCV) nonstructural proteins, as well as viral plus-strand RNA, in Huh-7 cells harboring autonomously replicating subgenomic HCV RNAs. Metabolic labeling with 5-bromouridine 5′-triphosphate in the presence of actinomycin D revealed that the membranous web is the site of

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Makel, Matthew C., Jonathan A. Plucker, Jennifer Freeman, Allison Lombardi, Brandi Simonsen, and Michael Coyne. "Replication of Special Education Research." Remedial and Special Education 37, no. 4 (2016): 205–12. http://dx.doi.org/10.1177/0741932516646083.

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Increased calls for rigor in special education have often revolved around the use of experimental research design. However, the replicability of research results is also a central tenet to the scientific research process. To assess the prevalence, success rate, and authorship history of replications in special education, we investigated the complete publication history of every replication published in the 36 journals categorized by ISI Web of Knowledge Journal Citation Report as special education. We found that 0.5% of all articles reported seeking to replicate a previously published finding.

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Pan, Zhijian, and James A. Reggia. "Computational Discovery of Instructionless Self-Replicating Structures in Cellular Automata." Artificial Life 16, no. 1 (2010): 39–63. http://dx.doi.org/10.1162/artl.2009.16.1.16104.

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Cellular automata models have historically been a major approach to studying the information-processing properties of self-replication. Here we explore the feasibility of adopting genetic programming so that, when it is given a fairly arbitrary initial cellular automata configuration, it will automatically generate a set of rules that make the given configuration replicate. We found that this approach works surprisingly effectively for structures as large as 50 components or more. The replication mechanisms discovered by genetic programming work quite differently than those of many past manual

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Krude, T., C. Musahl, R. A. Laskey, and R. Knippers. "Human replication proteins hCdc21, hCdc46 and P1Mcm3 bind chromatin uniformly before S-phase and are displaced locally during DNA replication." Journal of Cell Science 109, no. 2 (1996): 309–18. http://dx.doi.org/10.1242/jcs.109.2.309.

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Members of the Mcm-protein family have recently been shown to be involved in restricting DNA replication to a single cycle in Xenopus laevis egg extracts. In this study, we extended these observations to human somatic cells and analysed the localisation of the human Mcm-proteins Cdc21, Cdc46 and P1Mcm3 in replicating HeLa cell nuclei. These Mcm-proteins are entirely nuclear in interphase cells and apparently exist in two populations: a nucleosolic population, and a population bound to a nuclear structure, most likely chromatin. The bound population is detected throughout the nucleus in late G1

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Haase, S. B., S. S. Heinzel, and M. P. Calos. "Transcription inhibits the replication of autonomously replicating plasmids in human cells." Molecular and Cellular Biology 14, no. 4 (1994): 2516–24. http://dx.doi.org/10.1128/mcb.14.4.2516-2524.1994.

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This study addresses the effect of transcription on replication, using a system based on autonomously replicating plasmids in human cells. We added transcriptional elements from the human cytomegalovirus promoter/enhancer and the human beta-actin promoter to autonomously replicating plasmids based on human sequences and found that the transcriptional elements inhibited plasmid replication. Furthermore, conditional inhibition of plasmid replication was demonstrated by using a tetracycline-responsive promoter. We found that replication activity of plasmids carrying this promoter was inversely co

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Haase, S. B., S. S. Heinzel, and M. P. Calos. "Transcription inhibits the replication of autonomously replicating plasmids in human cells." Molecular and Cellular Biology 14, no. 4 (1994): 2516–24. http://dx.doi.org/10.1128/mcb.14.4.2516.

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This study addresses the effect of transcription on replication, using a system based on autonomously replicating plasmids in human cells. We added transcriptional elements from the human cytomegalovirus promoter/enhancer and the human beta-actin promoter to autonomously replicating plasmids based on human sequences and found that the transcriptional elements inhibited plasmid replication. Furthermore, conditional inhibition of plasmid replication was demonstrated by using a tetracycline-responsive promoter. We found that replication activity of plasmids carrying this promoter was inversely co

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Goldstein, Joshua N., and Sandra K. Weller. "In Vitro Processing of Herpes Simplex Virus Type 1 DNA Replication Intermediates by the Viral Alkaline Nuclease, UL12." Journal of Virology 72, no. 11 (1998): 8772–81. http://dx.doi.org/10.1128/jvi.72.11.8772-8781.1998.

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ABSTRACT Herpes simplex virus type 1 (HSV-1) DNA replication intermediates exist in a complex nonlinear structure that does not migrate into a pulsed-field gel. Genetic evidence suggests that the product of the UL12 gene, termed alkaline nuclease, plays a role in processing replication intermediates (R. Martinez, R. T. Sarisky, P. C. Weber, and S. K. Weller, J. Virol. 70:2075–2085, 1996). In this study we have tested the hypothesis that alkaline nuclease acts as a structure-specific resolvase. Cruciform structures generated with oligonucleotides were treated with purified alkaline nuclease; ho

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Scholle, Frank, Kui Li, Francis Bodola, Masanori Ikeda, Bruce A. Luxon, and Stanley M. Lemon. "Virus-Host Cell Interactions during Hepatitis C Virus RNA Replication: Impact of Polyprotein Expression on the Cellular Transcriptome and Cell Cycle Association with Viral RNA Synthesis." Journal of Virology 78, no. 3 (2004): 1513–24. http://dx.doi.org/10.1128/jvi.78.3.1513-1524.2004.

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ABSTRACT Considerable controversy surrounds the impact of hepatitis C virus (HCV) protein expression on viability of host cells and regulation of the cell cycle. Both promotion of cellular proliferation and apoptosis have been observed in different experimental systems. To determine whether expression of the entire complement of HCV proteins in the context of ongoing viral RNA replication significantly alters the host cell transcriptome and cell cycle regulatory processes, we carried out high-density oligonucleotide microarray studies and analyzed cell cycle distributions and S-phase entry in

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Srutkowska, S., G. Konopa, and G. Wegrzyn. "A method for isolation of plasmid DNA replication intermediates from unsynchronized bacterial cultures for electron microscopy analysis." Acta Biochimica Polonica 45, no. 1 (1998): 233–40. http://dx.doi.org/10.18388/abp.1998_4305.

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Electron microscopy is a powerful technique for analysis of DNA replication intermediates. However, isolation of replicating DNA molecules from living cells is tricky and difficult, especially in the case of small DNA molecules (such as bacterial plasmids) whose initiation of replication is not easily synchronized. Here a relatively simple and rapid method for efficient isolation of replicating plasmid molecules from unsynchronized Escherichia coli cultures is described. The efficiency of this procedure is high enough for electron microscopy analysis of plasmid replication intermediates appear

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McCaskill, John S., Norman H. Packard, Steen Rasmussen, and Mark A. Bedau. "Evolutionary self-organization in complex fluids." Philosophical Transactions of the Royal Society B: Biological Sciences 362, no. 1486 (2007): 1763–79. http://dx.doi.org/10.1098/rstb.2007.2069.

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This paper explores the ability of molecular evolution to take control of collective physical phases, making the first decisive step from independent replicators towards cell-like collective structures. We develop a physical model of replicating combinatorial molecules in a ternary fluid of hydrocarbons, amphiphiles and water. Such systems are being studied experimentally in various laboratories to approach the synthesis of artificial cells, and are also relevant to the origin of cellular life. The model represents amphiphiles by spins on a lattice (with Ising coupling in the simplest case), c

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Wu, Rong, Prim B. Singh, and David M. Gilbert. "Uncoupling global and fine-tuning replication timing determinants for mouse pericentric heterochromatin." Journal of Cell Biology 174, no. 2 (2006): 185–94. http://dx.doi.org/10.1083/jcb.200601113.

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Mouse chromocenters are clusters of late-replicating pericentric heterochromatin containing HP1 bound to trimethylated lysine 9 of histone H3 (Me3K9H3). Using a cell-free system to initiate replication within G1-phase nuclei, we demonstrate that chromocenters acquire the property of late replication coincident with their reorganization after mitosis and the establishment of a global replication timing program. HP1 dissociated during mitosis but rebound before the establishment of late replication, and removing HP1 from chromocenters by competition with Me3K9H3 peptides did not result in early

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Wanka, F. "Functional aspects of the nuclear matrix." Acta Biochimica Polonica 42, no. 2 (1995): 127–31. http://dx.doi.org/10.18388/abp.1995_4599.

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A model is proposed of the way in which the unwinding of the chromosomal DNA loops is controlled during DNA replication. It is based on the observation of a permanent binding of replication origins to the nuclear matrix and of a transient attachment of replicating DNA regions to sites in the immediate neighbourhood. DNA unwinding is controlled while the replicating loops are reeled through the replication binding sites. Also a mechanism is proposed to explain how the once-per-cycle replication of individual replicons can be controlled. DNA synthesis is initiated at single-stranded loops expose

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Tunin, Karen, and Priscila Guimarães Otto. "Late-replicating X-chromosome: replication patterns in mammalian females." Genetics and Molecular Biology 25, no. 3 (2002): 305–8. http://dx.doi.org/10.1590/s1415-47572002000300009.

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Silverman, W. A. "Replicating Measurements." PEDIATRICS 112, no. 2 (2003): 415–16. http://dx.doi.org/10.1542/peds.112.2.415.

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Burk, N. M. "Replicating Earth." IEEE Potentials 14, no. 2 (1995): 32–33. http://dx.doi.org/10.1109/45.376644.

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