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Auswahl der wissenschaftlichen Literatur zum Thema „Rhamnolipides (RLs)“
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Zeitschriftenartikel zum Thema "Rhamnolipides (RLs)"
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Chebbi, Alif, Massimiliano Tazzari, Cristiana Rizzi, Franco Hernan Gomez Tovar, Sara Villa, Silvia Sbaffoni, Mentore Vaccari und Andrea Franzetti. „Burkholderia thailandensis E264 as a promising safe rhamnolipids’ producer towards a sustainable valorization of grape marcs and olive mill pomace“. Applied Microbiology and Biotechnology 105, Nr. 9 (20.04.2021): 3825–42. http://dx.doi.org/10.1007/s00253-021-11292-0.
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Abstract Within the circular economy framework, our study aims to assess the rhamnolipid production from winery and olive oil residues as low-cost carbon sources by nonpathogenic strains. After evaluating various agricultural residues from those two sectors, Burkholderia thailandensis E264 was found to use the raw soluble fraction of nonfermented (white) grape marcs (NF), as the sole carbon and energy source, and simultaneously, reducing the surface tension to around 35 mN/m. Interestingly, this strain showed a rhamnolipid production up to 1070 mg/L (13.37 mg/g of NF), with a higher purity, on those grape marcs, predominately Rha-Rha C14-C14, in MSM medium. On olive oil residues, the rhamnolipid yield of using olive mill pomace (OMP) at 2% (w/v) was around 300 mg/L (15 mg/g of OMP) with a similar CMC of 500 mg/L. To the best of our knowledge, our study indicated for the first time that a nonpathogenic bacterium is able to produce long-chain rhamnolipids in MSM medium supplemented with winery residues, as sole carbon and energy source. Key points • Winery and olive oil residues are used for producing long-chain rhamnolipids (RLs). • Both higher RL yields and purity were obtained on nonfermented grape marcs as substrates. • Long-chain RLs revealed stabilities over a wide range of pH, temperatures, and salinities
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Mishra, Alok K., Rikesh K. Dubey, Shivraj M. Yabaji und Swati Jaiswal. „Evaluation of antimycobacterial rhamnolipid production from non-cytotoxic strains of Pseudomonas aeruginosa isolated from rhizospheric soil of medicinal plants“. International Journal of Biological Research 4, Nr. 2 (06.08.2016): 112. http://dx.doi.org/10.14419/ijbr.v4i2.6429.
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Rhamnolipids (RLs) are the bacterial derived biosurfactants and known for a wide range of industrial and therapeutic applications. They exhibit potent anti-bacterial activity against various gram positive, gram negative and acid fast bacteria including Mycobacterium tuberculosis. Since, Pseudomonas is one of the largest known genuses containing a variety of rhamnolipid producing strains. Therefore, in this study, we selectively isolated the Pseudomonas aeruginosa strains from the rhizospheric soil of the Indian plants of medicinal value, e.g. Azadirachta Indica and Ficus spp., and evaluated them for their natural ability to produce antibacterial rhamnolipids. The bacteria were identified on the basis of 16s rRNA sequencing and biochemical characterization. Among 33 of P. aeruginosa isolates from different soil samples, four isolates showed potent inhibitory activity against methicillin resistant Staphylococcus aureus (MRSA) and fast grower mycobacterial spp. The inhibitory potential of the isolates was found to be correlated with their ability to produce RLs in the medium. The industrial viability of the strains was assessed on the basis of cytotoxicity determining alternative allele, exoS/exoU and cell mediated cytotoxicity against murine macrophages J774.1. The newly isolated strains harbor exoS allele and exhibits lower cell mediated cytotoxicity on macrophage cell line as compared to the clinical strains PA-BAA-427 and PA-27853 used as a control in this study.Evaluation of antimycobacterial rhamnolipid production from non-cytotoxic strains of Pseudomonas aeruginosa isolated from rhizospheric soil of medicinal plants
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Yoshimura, Ingrid, Ana Maria Salazar-Bryam, Adriano Uemura de Faria, Lucas Prado Leite, Roberta Barros Lovaglio und Jonas Contiero. „Guava Seed Oil: Potential Waste for the Rhamnolipids Production“. Fermentation 8, Nr. 8 (09.08.2022): 379. http://dx.doi.org/10.3390/fermentation8080379.
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Guava is consumed in natura and is also of considerable importance to the food industry. The seeds and peel of this fruit are discarded, however, guava seeds yield oil (~13%) that can be used for the bioproducts synthesis. The use of a by-product as a carbon source is advantageous, as it reduces the environmental impact of possible harmful materials to nature, while adding value to products. In addition, the use of untested substrates can bring new yield and characterization results. Thus, this research sought to study rhamnolipids (RLs) production from guava seed oil, a by-product of the fructorefinery. The experiments were carried out using Pseudomonas aeruginosa LBI 2A1 and experimental design was used to optimize the variables Carbon and Nitrogen concentration. Characterization of RLs produced occurred by LC-MS. In this study, variables in the quadratic forms and the interaction between them influenced the response (p < 0.05). The most significant variable was N concentration. Maximum RLs yield achieved 39.97 g/L, predominantly of mono-RL. Characterization analysis revealed 9 homologues including the presence of RhaC10C14:2 (m/z 555) whose structure has not previously been observed. This research showed that guava seed oil is an alternative potential carbon source for rhamnolipid production with rare rhamnolipid homologues.
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Zhang, Haoran, Xiaorong Yu, Qing Li, Guangtian Cao, Jie Feng, Yuanyuan Shen und Caimei Yang. „Effects of Rhamnolipids on Growth Performance, Immune Function, and Cecal Microflora in Linnan Yellow Broilers Challenged with Lipopolysaccharides“. Antibiotics 10, Nr. 8 (24.07.2021): 905. http://dx.doi.org/10.3390/antibiotics10080905.
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This present study aimed to investigate the effects of rhamnolipids (RLS) on the growth performance, intestinal morphology, immune function, short-chain fatty acid content, and microflora community in broiler chickens challenged with lipopolysaccharides (LPS). A total of 450 broiler chickens were randomly allocated into three groups: basal diet with no supplement (NCO), basal diet with bacitracin (ANT), and basal diet with rhamnolipids (RLS). After 56 d of feeding, 20 healthy broilers were selected from each group, with half being intraperitoneally injected with lipopolysaccharides (LPS) and the other half with normal saline. Treatments with LPS were labelled LPS-NCO, LPS-ANT, and LPS-RLS, whereas treatments with normal saline were labelled NS-NCO, NS-ANT, and NS-RLS. LPS-challenged birds had lower jejunal villus height and higher crypt depth than unchallenged birds. LPS-RLS broilers had increased jejunal villus height and villus height/crypt depth ratio (V/C) but lower crypt depth than LPS-NCO. Dietary supplementation with RLS reduced the LPS-induced immunological stress. Compared with LPS-NCO, birds in LPS-RLS had lower concentrations of IL-1β, IL-6, and TNF-α. In LPS-challenged broilers, RLS and ANT increased the concentrations of IgA, IgM, and IgY compared with LPS-NCO. In LPS treatments, RLS enhanced the contents of acetic acid, butyrate, isobutyric acid, isovalerate, and valerate more than LPS-NCO birds. High-throughput sequencing indicated that RLS supplementation led to changes in the cecal microbial community of broilers. At the species level, Clostridium-sp-Marseille-p3244 and Slakia_eqcsolifaciens were more abundant in NS-RLS than in NS-NCO broilers. In summary, RLS improved the growth performance and relative abundance of cecal microbiota and reduced the LPS-induced immunological stress in broiler chickens.
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Semkova, Severina, Georgi Antov, Ivan Iliev, Iana Tsoneva, Pavel Lefterov, Nelly Christova, Lilyana Nacheva et al. „Rhamnolipid Biosurfactants—Possible Natural Anticancer Agents and Autophagy Inhibitors“. Separations 8, Nr. 7 (28.06.2021): 92. http://dx.doi.org/10.3390/separations8070092.
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Background/Aim: A number of biologically active substances were proved as an alternative to conventional anticancer medicines. The aim of the study is in vitro investigation of the anticancer activity of mono- and di-Rhamnolipids (RL-1 and RL-2) against human breast cancer. Additionally, the combination with Cisplatin was analyzed. Materials and Methods: Breast cell lines (MCF-10A, MCF-7 and MDA-MB-231) were treated with RLs and in combination with Cisplatin. The viability was analyzed using MTT assay, and investigation of autophagy was performed via acridine orange staining. Results: In contrast to the healthy cells, both tested cancer lines exhibited sensitivity to RLs treatment. This effect was accompanied by an influence on the autophagy-related acidic formation process. Only for the triple-negative breast cancer cell line (MDA-MB-231) the synergistic effect of the combined treatment (10 µM Cisplatin and 1 µg/mL RL-2) was observed. Conclusion: Based on studies on the reorganization of membrane models in the presence of RL and the data about a higher amount of lipid rafts in cancer cell membranes than in non-tumorigenic, we suggest a possible mechanism of membrane remodelling by formation of endosomes. Shortly, in order to have a synergistic effect, it is necessary to have Cisplatin andRL-2 as RL2 is a molecule inducingpositive membrane curvature.
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Sobri, Izuani Mohamad, Murni Halim, Oi-Ming Lai, Ahmad Firdaus Lajis, Mohd Termizi Yusof, Mohd Izuan Effendi Halmi, Wan Lutfi Wan Johari und Helmi Wasoh. „Emulsification Characteristics of Rhamnolipids by Pseudomonas aeruginosa Using Coconut Oil as Carbon Source“. Journal of Environmental Microbiology and Toxicology 6, Nr. 1 (31.07.2018): 7–12. http://dx.doi.org/10.54987/jemat.v6i1.400.
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Rhamnolipids (RLs) production using coconut oil as a carbon source by the bacterium P. aeruginosa is studied. This bacterium was grown in media containing 1% carbon source (glucose/ coconut oil). The RLs were characterized by emulsification index (E24), thermal stability and oil spreading test. Further RLs quantification was carried out by the orcinol assay with L-rhamnose as the standard. The result showed that the highest production of RLs occurred in the presence of both coconut oil and glucose at 96 h (2.51 g/L). A stable emulsification index (E24) was observed using diesel with a maximum value of 57% at room temperature. Good stability to high temperature (120 oC) was observed when exposed at 55%. Oil displacement activity showed the presence of RLs with the highest value was at the highest RLs production. This study shows P. aeruginosa is able to produce RLs using coconut oil as the substrate and may potentially become a good source of biosurfactant for industry in the future.
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Zahidullah, Zahidullah, Muhammad Faisal Siddiqui, Shamas Tabraiz, Farhana Maqbool, Fazal Adnan, Ihsan Ullah, Muhammad Ajmal Shah et al. „Targeting Microbial Biofouling by Controlling Biofilm Formation and Dispersal Using Rhamnolipids on RO Membrane“. Membranes 12, Nr. 10 (25.09.2022): 928. http://dx.doi.org/10.3390/membranes12100928.
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Finding new biological ways to control biofouling of the membrane in reverse osmosis (RO) is an important substitute for synthetic chemicals in the water industry. Here, the study was focused on the antimicrobial, biofilm formation, and biofilm dispersal potential of rhamnolipids (RLs) (biosurfactants). The MTT assay was also carried out to evaluate the effect of RLs on biofilm viability. Biofilm was qualitatively and quantitatively assessed by crystal violet assay, light microscopy, fluorescence microscopy (bacterial biomass (µm2), surface coverage (%)), and extracellular polymeric substances (EPSs). It was exhibited that RLs can reduce bacterial growth. The higher concentrations (≥100 mg/L) markedly reduced bacterial growth and biofilm formation, while RLs exhibited substantial dispersal effects (89.10% reduction) on preformed biofilms. Further, RLs exhibited 79.24% biomass reduction while polysaccharide was reduced to 60.55 µg/mL (p < 0.05) and protein to 4.67 µg/mL (p < 0.05). Light microscopy revealed biofilm reduction, which was confirmed using fluorescence microscopy. Microscopic images were processed with BioImageL software. It was revealed that biomass surface coverage was reduced to 1.1% at 1000 mg/L of RLs and that 43,245 µm2 of biomass was present for control, while biomass was reduced to 493 µm2 at 1000 mg/L of RLs. Thus, these data suggest that RLs have antimicrobial, biofilm control, and dispersal potential against membrane biofouling.
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Wasoh, Helmi, Sarinah Baharun, Murni Halim, Ahmad Firdaus Lajis, Arbakariya Ariff und Oi-Ming Lai. „Production of rhamnolipids by locally isolated Pseudomonas aeruginosa using sunflower oil as carbon source“. Bioremediation Science and Technology Research 5, Nr. 1 (31.07.2017): 1–6. http://dx.doi.org/10.54987/bstr.v5i1.350.
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Biosurfactants are surface active compounds and amphiphatic in nature which consist of hydrophilic head and hydrophobic tail accumulating at the interphase of two immiscible liquid with different polarity. A study was conducted to investigate the effectiveness of sunflower oil in the production of rhamnolipids (RLs) by locally isolated Pseudomonas aeruginosa in shake flask fermentation. In this process, four different fermentation treatments were done for seven days at 30°C and 180 rpm. Sampling was carried out in time intervals of 24 h followed by monitoring of cell growth and biosurfactants production. Colorimetric Orcinol analysis was used for determination of RLs concentrations (g/L). The RLs were studied for emulsification activity using emulsification index (E24%) methods. In addition, oil displacement activity and thermal stability were also studied (4-120°C). All treatments allow the growth of P. aeruginosa and the utilization of sunflower oil as carbon source and glucose as growth initiator were observed to be the best strategy for maximum RLs production. The maximum RLs production was achieved after 120 h with 3.18 g/L of RLs. Diesel shows the highest emulsification activity among the substrate tested ranging from 55.56% - 60.00%. The oil displacement activity was corresponding to RLs concentration with stability up to 120°C (for 60 min). Therefore, from this research a good potential of RLs that may provide good application for industry were produced.
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Giugliano, Rosa, Carmine Buonocore, Carla Zannella, Annalisa Chianese, Fortunato Palma Esposito, Pietro Tedesco, Anna De Filippis, Massimiliano Galdiero, Gianluigi Franci und Donatella de Pascale. „Antiviral Activity of the Rhamnolipids Mixture from the Antarctic Bacterium Pseudomonas gessardii M15 against Herpes Simplex Viruses and Coronaviruses“. Pharmaceutics 13, Nr. 12 (08.12.2021): 2121. http://dx.doi.org/10.3390/pharmaceutics13122121.
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Emerging and re-emerging viruses represent a serious threat to human health at a global level. In particular, enveloped viruses are one of the main causes of viral outbreaks, as recently demonstrated by SARS-CoV-2. An effective strategy to counteract these viruses could be to target the envelope by using surface-active compounds. Rhamnolipids (RLs) are microbial biosurfactants displaying a wide range of bioactivities, such as antibacterial, antifungal and antibiofilm, among others. Being of microbial origin, they are environmentally-friendly, biodegradable, and less toxic than synthetic surfactants. In this work, we explored the antiviral activity of the rhamnolipids mixture (M15RL) produced by the Antarctic bacteria Pseudomonas gessardii M15 against viruses belonging to Coronaviridae and Herpesviridae families. In addition, we investigated the rhamnolipids’ mode of action and the possibility of inactivating viruses on treated surfaces. Our results show complete inactivation of HSV-1 and HSV-2 by M15RLs at 6 µg/mL, and of HCoV-229E and SARS-CoV-2 at 25 and 50 µg/mL, respectively. Concerning activity against HCoV-OC43, 80% inhibition of cytopathic effect was recorded, while no activity against naked Poliovirus Type 1 (PV-1) was detectable, suggesting that the antiviral action is mainly directed towards the envelope. In conclusion, we report a significant activity of M15RL against enveloped viruses and demonstrated for the first time the antiviral effect of rhamnolipids against SARS-CoV-2.
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Sharma, Pushpinder, und Nivedita Sharma. „Microbial Biosurfactants-an Ecofriendly Boon to Industries for Green Revolution“. Recent Patents on Biotechnology 14, Nr. 3 (25.09.2020): 169–83. http://dx.doi.org/10.2174/1872208313666191212094628.
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Biosurfactants have a biological origin, and are widely known as surface active agents. Different classes of biosurfactant have significant importance in both the biotechnological and microbiological arena. Pseudomonas aeruginosa, Bacillus subtilis and Candida sp. are important classes of microorganisms that are highly investigated for the production of rhamnolipids (RLs) biosurfactants. Rhamnolipids have unique surface activity and have gained interest in various industrial applications. Due to their high biodegradability, renewability and functionally maintenance at extreme conditions, microbial biosurfactants are more advantageous than chemical-based biosurfactants. Biosurfactants produced by microorganisms are a potential candidate for biodegradation, environmental cleanup of pollutants and also play a role in the heavy metal removal of metallurgical industries also many patents have been filed. Therefore, greater attention has been paid to biosurfactants and identifying their potential applications for further studies.
Dissertationen zum Thema "Rhamnolipides (RLs)"
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Botcazon, Camille. „Etude du mode d'action de composés antifongiques membranotropes naturels sur deux Sclerotiniacées : cas des rhamnolipides et des fengycines“. Electronic Thesis or Diss., Compiègne, 2023. https://bibliotheque.utc.fr/Default/doc/SYRACUSE/2023COMP2755.
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Les rhamnolipides (RLs) et les fengycines (FGs), des composés sécrétés par des bactéries, ont des propriétés antifongiques contre les champignons phytopathogènes Sclerotinia sclerotiorum et Botrytis cinerea. Cependant, les effets biocides induits et les mécanismes impliqués sont peu étudiés chez les champignons. De par leur caractère amphiphile, un mode d’action membranotrope est proposé pour ces composés intéréssants pour le biocontrôle. Les travaux présentés ici démontrent que les deux Sclérotiniacées ont des sensibilités opposées aux RLs et aux FGs. Une étude en microscopie montre que les RLs peuvent induire une mort cellulaire programmée (PCD) ou nécrotique chez les deux champignons selon la concentration alors que les FGs induisent systématiquement une PCD, probablement par un mécanisme d’autophagie. Des analyses lipidomiques (contenus en acides gras, phospholipides et ergostérol) de souches de S. sclerotiorum et B. cinerea plus ou moins sensibles aux RLs et aux FGs permettent de rapprocher les contenus lipidiques des champignons à leurs sensibilités. Ces données ont été utilisées pour étudier les interactions entre les RLs ou les FGs et des modèles de membranes plasmiques biomimétiques des deux champignons. Les études de dynamique moléculaire montrent que les RLs s’insèrent, sous forme de monomères, dans les différents modèles étudiés sans les fluidifier et que les FGs s’agrègent puis s’insèrent dans certains modèles en induisant une fluidification. L’ergostérol et les acides phosphatidiques défavoriseraient cette insertion tandis que les phosphatidylcholines et les phosphatidyléthanolamines les favoriseraient. Ces travaux permettent de mieux appréhender le mode d’action antifongique des RLs et des FGs, et contribueront, à terme, au développement de produits de biocontrôle plus efficaces pour la protection des cultures vis-à-vis de pathogènes spécifiquesRhamnolipids (RLs) and fengycins (FGs), are compounds produced by bacteria displaying antifungal properties against the phytopathogenic fungi Sclerotinia sclerotiorum and Botrytis cinerea. However, the induced biocidal effects, and the involved mechanisms are poorly understood in fungi. Due to their amphiphilic properties, a membranotropic mode of action is proposed for these interesting compounds for biocontrol. The present work demonstrates that the two Sclerotiniaceae have opposite sensitivities to RLs and FGs. A microscopy study shows that RLs can induce programmed cell death (PCD) or necrotic cell death in both fungi depending on the concentration whereas FGs systematically induce PCD, probably by triggering autophagy. Lipidomic analyses (fatty acid, phospholipid and ergosterol contents) of S. sclerotiorum and B. cinerea strains differently sensitive to RLs and FGs allow to correlate the lipid contents of the fungi to their sensitivities. These data are used to study the interactions of RLs or FGs on biomimetic plasma membrane models of the two fungi. The dynamics show that the RLs monomers insert into the models without fluidizing them and that the FGs auto-aggregate themselves and insert into some models, inducing fluidization. Ergosterol and phosphatidic acids seems to disfavour this insertion while phosphatidylcholine and phosphatidylethanolamine seem to favour it.This work allows to better understand the antifungal mode of action of RLs and FGs, with a view to develop more effective biocontrol products for crop protection targeting specific pathogens
Buchteile zum Thema "Rhamnolipides (RLs)"
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Devale, Anushka, Rupali Sawant, Karishma Pardesi, Surekha Satpute und Shilpa Mujumdar. „Rhamnolipids (RLs) Green Solution for Global Hydrocarbon Pollution“. In Microbial Surfactants, 208–25. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9781003260165-12.
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