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Thematische Bibliographien / Thin layer Chromatography-Direct Bioautography

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Konferenzberichte

Auswahl der wissenschaftlichen Literatur zum Thema „Thin layer Chromatography-Direct Bioautography“

Autor: Grafiati

Veröffentlicht am 7. Juni 2025

Zuletzt aktualisiert am 2. August 2025

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Zeitschriftenartikel zum Thema "Thin layer Chromatography-Direct Bioautography"

1

Choma, I. M. "Thin‐Layer Chromatography‐Direct Bioautography of Flumequine Residues in Milk." Journal of Liquid Chromatography & Related Technologies 29, no. 14 (2006): 2083–93. http://dx.doi.org/10.1080/10826070600759942.

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2

Choma, Irena M., and Edyta M. Grzelak. "Bioautography detection in thin-layer chromatography." Journal of Chromatography A 1218, no. 19 (2011): 2684–91. http://dx.doi.org/10.1016/j.chroma.2010.12.069.

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3

Vishwa, Patel1* Niyati Acharya2. "Application of TLC Bioautography for Natural Bioactive Screening." International Journal of Pharmaceutical Sciences 3, no. 5 (2025): 1376–86. https://doi.org/10.5281/zenodo.15378848.

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Bioautography is a mean of targeted-directed isolation of active molecules on chromatogram. Bioautography is technique to detect substances affecting the growth rates to test organisms in complex mixtures and matrices. The antimicrobial, antioxidant and enzyme inhibitory activities can be performed on TLC bioautography. Bioautography methods are divided into three categories. 1)Agar diffusion or contact bioautography 2) Immersion or agar-overlay bioautography 3) Direct bioautography. High throughput method enabling analyses of many samples in parallel and the comparison of their activity. Both

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4

Anuar, Nadia, Sadia Sultan, and Kamran Ashraf. "An Overview of Antimicrobial and Antioxidant Bioautography Method Analysis: Cosmos caudatus and Orthosiphon stamineus." International Journal of Pharmaceuticals, Nutraceuticals and Cosmetic Science 5, no. 1 (2022): 1–12. http://dx.doi.org/10.24191/ijpnacs.v5i1.01.

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Medicinal herbs such as Cosmos caudatus and Orthosiphon stamineus are sources of a huge number of bioactive compounds with antioxidant and antimicrobial properties. C. caudatus plant has bioactive compounds such as phenolics, flavonoids, and terpenoids. On the other hand, phytochemicals from O. stamineus have been shown to have a wide range of traditional applications due to their antioxidant and antibacterial characteristics. The bioautography method is used to isolate active molecules on chromatogram followed by the chemical and biological detection system. In antimicrobial bioautography, ag

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5

Choma, Irena, Adam Choma, and Krystyna Staszczuk. "Direct bioautography-thin-layer chromatography of flumequine and doxycycline in milk." Journal of Planar Chromatography – Modern TLC 15, no. 3 (2002): 187–91. http://dx.doi.org/10.1556/jpc.15.2002.3.5.

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6

Choma, I. M., A. Choma, I. Komaniecka, K. Pilorz, and K. Staszczuk. "Semiquantitative Estimation of Enrofloxacin and Ciprofloxacin by Thin‐Layer Chromatography–Direct Bioautography." Journal of Liquid Chromatography & Related Technologies 27, no. 13 (2004): 2071–85. http://dx.doi.org/10.1081/jlc-120039419.

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7

Isnaeni, Isnaeni, Andri Astuti, and Muhammad Yuwono. "Validation of Thin-Layer Chromatography-Bioautographic Method for Determination of Streptomycin." JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA 4, no. 1 (2018): 34. http://dx.doi.org/10.20473/jfiki.v4i12017.34-38.

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Background: A simple bio-assay for determination of streptomycin hyphenated with planar chromatography techniques was developed. Objective: This study aims to validate the method for identification and determination of streptomycin in injection preparations with TLC-bioautography. Methods: Thin Layer Chromatography (TLC) was performed on the silica Gel GF-254 using KH2PO4 solution as mobile solvent. The visualization was performed by spraying 2% resorcinol. Direct bi autography was developed using Escherichia coli ATCC 25922 as a bacterial test, grown on the nutrient agar medium at 37oC for 24

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8

Balázs, Viktória Lilla, Barbara Horváth, Erika Kerekes, et al. "Anti-Haemophilus Activity of Selected Essential Oils Detected by TLC-Direct Bioautography and Biofilm Inhibition." Molecules 24, no. 18 (2019): 3301. http://dx.doi.org/10.3390/molecules24183301.

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Essential oils (EOs) are becoming increasingly popular in medical applications because of their antimicrobial effect. Direct bioautography (DB) combined with thin layer chromatography (TLC) is a screening method for the detection of antimicrobial compounds in plant extracts, for example, in EOs. Due to their lipophilic character, the common microbiological assays (etc. disk diffusion) could not provide reliable results. The aim of this study was the evaluation of antibacterial and anti-biofilm properties of the EO of cinnamon bark, clove, peppermint, thyme, and their main components against Ha

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9

Jesionek, Wioleta, Edyta Grzelak, Barbara Majer-Dziedzic, and Irena Choma. "Thin-layer chromatography — Direct bioautography for the screening of antimicrobial properties of plant extracts." Journal of Planar Chromatography – Modern TLC 26, no. 2 (2013): 109–13. http://dx.doi.org/10.1556/jpc.26.2013.2.1.

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10

Choma, I. M., A. Choma, and K. Staszczuk. "DETERMINATION OF FLUMEQUINE IN MILK BY THIN-LAYER CHROMATOGRAPHY-BIOAUTOGRAPHY." Journal of Liquid Chromatography & Related Technologies 25, no. 10-11 (2002): 1579–87. http://dx.doi.org/10.1081/jlc-120005705.

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Buchteile zum Thema "Thin layer Chromatography-Direct Bioautography"

1

Schiller, Jurgen, Beate Fuchs, Kristin Teuber, Ariane Nimptsch, Kathrin Nimptsch, and Rosmarie Süß. "TLC/HPTLC with Direct Mass Spectrometric Detection: A Review of the Progress Achieved in the Last 5 Years." In High-Performance Thin-Layer Chromatography (HPTLC). Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-14025-9_16.

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2

Sherma, Joseph. "Studies on the Antioxidant Activity of Foods and Food Ingredients by Thin-Layer Chromatography–Direct Bioautography with 2,2′-Diphenyl-1-Picrylhydrazyl Radical (DPPH*)." In Advances in Chromatography. CRC Press, 2017. http://dx.doi.org/10.1201/9781315158075-9.

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3

Gervasi, Teresa, and Giuseppina Mandalari. "Antimicrobial Activity Methods." In Methods for Preclinical Evaluation of Bioactive Natural Products. BENTHAM SCIENCE PUBLISHERS, 2023. http://dx.doi.org/10.2174/9789815123043123010006.

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Plant extracts are widely utilized for their antimicrobial effect, alone or in combination with traditional drugs. However, the current global lack of harmonization on the assays used to investigate their biological effect makes the result comparison very challenging. In the present chapter, we highlight the variables used for the antimicrobial testing, which may affect the end result. The in vitro antimicrobial methods, including diffusion methods, dilution methods and thin-layer chromatography-bioautography are reported, together with a range of assays aimed at evaluating the mechanisms of a

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Konferenzberichte zum Thema "Thin layer Chromatography-Direct Bioautography"

1

Sen, Ashis K., Jeff Darabi, Ranu Nayak, and Daniel R. Knapp. "Desorption Electrospray Ionization Using a Porous Alumina Surface." In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-176016.

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Desorption electrospray ionization (DESI) is a technique used for direct sampling of a sample or an analyte deposited on a surface under ambient conditions [1]. In DESI, ionized droplets of a spray are directed towards the sample causing desorption of ions due to exchange of charge and momentum. The resulting ions are carried into an ion trap mass spectrometer and analyzed. DESI was originally demonstrated by Takats et al. [1]. They described the new method and applied the same to analyze various compounds present on a variety of surfaces. Followed by this, several researchers [1–5] have inves

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