Auswahl der wissenschaftlichen Literatur zum Thema „TurboID“

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Zeitschriftenartikel zum Thema "TurboID"

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Cho, Kelvin F., Tess C. Branon, Sanjana Rajeev, et al. "Split-TurboID enables contact-dependent proximity labeling in cells." Proceedings of the National Academy of Sciences 117, no. 22 (2020): 12143–54. http://dx.doi.org/10.1073/pnas.1919528117.

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Proximity labeling catalyzed by promiscuous enzymes, such as TurboID, have enabled the proteomic analysis of subcellular regions difficult or impossible to access by conventional fractionation-based approaches. Yet some cellular regions, such as organelle contact sites, remain out of reach for current PL methods. To address this limitation, we split the enzyme TurboID into two inactive fragments that recombine when driven together by a protein–protein interaction or membrane–membrane apposition. At endoplasmic reticulum–mitochondria contact sites, reconstituted TurboID catalyzed spatially rest
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Cho, Kelvin F., Tess C. Branon, Namrata D. Udeshi, Samuel A. Myers, Steven A. Carr, and Alice Y. Ting. "Proximity labeling in mammalian cells with TurboID and split-TurboID." Nature Protocols 15, no. 12 (2020): 3971–99. http://dx.doi.org/10.1038/s41596-020-0399-0.

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May, Danielle G., Kelsey L. Scott, Alexandre R. Campos, and Kyle J. Roux. "Comparative Application of BioID and TurboID for Protein-Proximity Biotinylation." Cells 9, no. 5 (2020): 1070. http://dx.doi.org/10.3390/cells9051070.

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BioID is a well-established method for identifying protein–protein interactions and has been utilized within live cells and several animal models. However, the conventional labeling period requires 15–18 h for robust biotinylation which may not be ideal for some applications. Recently, two new ligases termed TurboID and miniTurbo were developed using directed evolution of the BioID ligase and were able to produce robust biotinylation following a 10 min incubation with excess biotin. However, there is reported concern about inducibility of biotinylation, cellular toxicity, and ligase stability.
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Doerr, Allison. "Proximity labeling with TurboID." Nature Methods 15, no. 10 (2018): 764. http://dx.doi.org/10.1038/s41592-018-0158-0.

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Garloff, Vera, and Ignacio Rubio. "Schneller, weiter, TurboID – Modulation einer übereifrigen Biotin-Ligase." BIOspektrum 29, no. 3 (2023): 273–75. http://dx.doi.org/10.1007/s12268-023-1943-6.

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AbstractProtein-protein interactions are key elements of intracellular signalling and metabolic pathways. These interactions can be revealed with the help of proximity ligation screens, prominently biotinylation screens. This approach has profited from the recent development of the highly active biotin ligase TurboID, which however also led to problems of toxicity related to its high basal activity. We have established a simple protocol to improve TurboID performance and enhance protein functionality.
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Makhsatova, S. A., A. B. Kurmanbay, I. A. Akhmetollayev, and A. T. Kulyyassov. "ASSEMBLING THE TURBOID-CONTAINING PLASMID CONSTRUCT FOR INVESTIGATING THE IN VIVO PROTEIN-PROTEIN INTERACTIONS." Eurasian Journal of Applied Biotechnology, no. 3S (September 12, 2024): 47. http://dx.doi.org/10.11134/btp.3s.2024.35.

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In vivo interactions between biomolecules (Proteins, RNA, and DNA) are the basis of cellular functionality including cell cycle, signaling pathways, cellular metabolism, and other biological processes. The traditional methods for detecting protein-protein interactions, such as affinity purification and two-hybrid analysis have limitations for the in-depth study of the cellular proteome. Besides, proteomics of the organelle protein components is still challenging to study, due to the spatial and temporal dynamics of proteins. To address these problems, proximity labeling technology was introduc
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Takano, Tetsuya. "Comprehensive identification of molecules at synapses and non-synaptic cell-adhesion structure." Impact 2023, no. 3 (2023): 46–48. http://dx.doi.org/10.21820/23987073.2023.3.46.

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The brain is incredibly complex and there is so much we don't know about this organ and its mechanisms. Assistant Professor Tetsuya Takano, School of Medicine, Keio University, Japan, is working to better understand neuroscience. One area of interest is neurons and astrocytes; specifically elucidating the protein component functions in each neural circuit. He and his team are working to shed light on the pathological mechanism of psychiatric and neurological disorders and, in doing so, enabling improved treatments and benefiting patients across the globe. The team has developed spatio-temporal
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Russo, Marissa, Emily Norton-Ramos, Maria Jose Ulloa Navas, Alfredo Quinones-Hinojosa, and Hugo Guerrero-Cazares. "Abstract 6579: Elucidating glioblastoma-derived extracellular vesicle cargo using TurboID: Implications for tumor microenvironment adaptation." Cancer Research 85, no. 8_Supplement_1 (2025): 6579. https://doi.org/10.1158/1538-7445.am2025-6579.

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Abstract Glioblastoma (GBM), the most aggressive primary brain tumor in adults, presents significant challenges due to its universal recurrence and limited survival rates, exacerbated when proximal to the lateral ventricles (LV). The subpopulation of brain tumor-initiating cells (BTICs) plays a pivotal role in tumor initiation and invasiveness, interacting with the microenvironment, particularly the cellular components in the subventricular zone (SVZ). Some mechanisms of intercellular communication present in the SVZ include paracrine, autocrine, direct cell contact, gap junctions, nanotubes,
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Rabinovich-Ernst, Orna, Clinton Bradfield, SungHwan Yoon, et al. "TurboID biotin-tagging mass spectrometry identifies specific caspase-11-associated proteins regulating non-canonical inflammasome activation." Journal of Immunology 206, no. 1_Supplement (2021): 15.06. http://dx.doi.org/10.4049/jimmunol.206.supp.15.06.

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Abstract While it has been demonstrated that cytosolic LPS can directly activate caspase11, the cellular processes regulating the non-canonical inflammasome response remain poorly defined. Caspase11 and caspase1 show substantial structural similarity, however, unlike the activation of caspase1 by NLR inflammasomes, there are no sensor or adaptor proteins known to be involved in transmitting cytosolic LPS signal to caspase11. Also, while caspase11 has been shown to associate with LPS, it lacks a characteristic LPS binding domain as observed in many other LPS binding proteins such as MD2 and LBP
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Kim, Han Byeol, and Kwang-eun Kim. "Precision proteomics with TurboID: mapping the suborganelle landscape." Korean Journal of Physiology & Pharmacology 28, no. 6 (2024): 495–501. http://dx.doi.org/10.4196/kjpp.2024.28.6.495.

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Dissertationen zum Thema "TurboID"

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Branon, Tess C. "Directed evolution of TurboID for efficient proximity labeling in living cells and organisms." Thesis, Massachusetts Institute of Technology, 2018. http://hdl.handle.net/1721.1/120909.

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Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemistry, 2018.<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references.<br>Protein interaction networks and protein compartmentalization underlie all signaling and regulatory processes in cells. Traditional approaches to proteomics employ mass spectrometry (MS) coupled to biochemical fractionation or affinity purification but require cell lysis prior to analysis which often results in false-negatives from missed interactions or incomplete purification and false-positives from contaminants. Enzyme-cat
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Falco, Marta. "Mechanistic insights into the EGFR-STK10 pathway in chronic kidney disease." Electronic Thesis or Diss., Université Paris Cité, 2024. https://wo.app.u-paris.fr/cgi-bin/WebObjects/TheseWeb.woa/wa/show?t=6583&f=76942.

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La maladie rénale chronique (MRC) représente un lourd fardeau pour la santé publique, et pourtant, les mécanismes moléculaires qui régissent la progression de la maladie restent mal compris. Parmi les voies de signalisation potentielles, la voie du récepteur du facteur de croissance épidermique (EGFR) joue un rôle crucial dans la progression de la MRC. Cependant, une inhibition prolongée de l'EGFR n'est pas une option envisageable pour les patients atteints de MRC en raison du risque d'effets indésirables importants. Une compréhension approfondie de la signalisation de l'EGFR est essentielle p
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Hajj, Sleiman Nawal. "Approche par nanobody pour capturer les interactomes de complexes protéiques dimériques en contexte cellulaire vivant." Electronic Thesis or Diss., Lyon, École normale supérieure, 2024. http://www.theses.fr/2024ENSL0041.

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L’identité et le devenir de chaque cellule dépend du contenu en protéines et, en particulier, des réseaux d'interactions protéine-protéine (IPP, également appelés interactomes). Les protéines ont la propriété générale de s'engager dans des assemblages macromoléculaires très variés, chacun ayant des fonctions bien distinctes. Par conséquent, identifier les IPP et les lier à des complexes particuliers est un enjeu crucial mais difficile en biologie. Cette problématique a été au cœur de mon travail de doctorat. Une première partie de mon travail est dédiée à l'amélioration d'une méthode existante
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Lilliesköld, Anders. "Genomgång av Turbomin 100 : Förstudie och föreslagna förbättringar av undervisningsjetmotor Turbomin 100." Thesis, Mälardalens högskola, Akademin för innovation, design och teknik, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-11195.

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ABSTRACT This project thesis has been written at the request of Mälardalens University, Västerås. The aeronautical engineering students at Mälardalens University and the pupils of Hässlö upper secondary school, all gets the opportunity to perform a computation lab with a real turbojet engine during their study. The goal of the lab from the University is that it should give the students applied experience from the theory part of which has been tought in the course “Aircraft Engine Technology”.                       The pupils of the Hässlö upper secondary school are performing simpler calculati
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Peiponen, K. E. (Kai-Erik). "Optical spectra analysis of turbid liquids." Doctoral thesis, University of Oulu, 2009. http://urn.fi/urn:isbn:9789514291685.

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Abstract This thesis is devoted to methods of analyzing optical spectra obtained from turbid liquids, i.e., liquids that are optically very thick and/or scatter light. Data for spectral analysis were obtained with a new, multifunction spectrophotometer developed for industrial liquid samples. One characteristic of the spectrophotometer is that spectral analysis methods can be implemented into the software. Here, the emphasis was on data inversion methods, particularly the Kramers-Kronig analysis and the maximum entropy method, which can be used to gain information on the wavelength-dependent c
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Esmonde-White, Francis. "Robust spectroscopic quantification in turbid media." Thesis, McGill University, 2009. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=32512.

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This thesis explores four methods for improving quantitative diffuse reflectance spectroscopy in light scattering media. In the introduction theories of light propagation in scattering media, relevant instrumentation for measuring light scattering properties, spectral data processing methods, and spectroscopically active bioanalytes are outlined. Next, two novel instruments for practical scattering measurements, and two novel data processing techniques are presented. Finally, directions for future researc
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Doan, Thuy Kim Phuong. "Fonctionnement biogéochimique d'un barrage tropical : application au système turbide de Cointzio (Mexique)." Thesis, Grenoble, 2014. http://www.theses.fr/2014GRENU011/document.

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La qualité globale des eaux continentales (lacs et réservoirs) continue à se détériorer dans de nombreuses régions du Mexique. Le réservoir Cointzio, situé dans la partie sud du Plateau Central Mexicain, sur la ceinture “Trans-Mexicain Volcanique Belt » (TMVB), ne fait pas exception. Ce réservoir tropical turbide est un système monomictique chaud (surface = 6 km2, capacité = 66 Mm3, temps de séjour ~ 1 an). Il est stratégique pour l'approvisionnement en eau potable de la ville de Morelia, capitale de l'état du Michoacán, et pour l'irrigation en aval pendant la saison sèche. Le réservoir est me
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Pour, Moghadam Parsa, and Kiarash Khaksa. "Underhåll av Turbomin 100." Thesis, Mälardalens högskola, Akademin för innovation, design och teknik, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-21643.

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Abstract Students at Mälardalen University have long performed their lab on a small turbojet engine in the course Aircraft Engine Technology. Since 2011, the engine has been dysfunctional. This was due to lack of maintenance. Since the lab has been an important element in teaching, the University had decided to get the engine functional again. This thesis project has been a request of Mälardalen University. The task we were assigned to was: 1. To make the engine functional. 2. Make the engine and the measuring equipment mobile. 3. Writing a brief maintenance program, such as what needs to be d
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Ducay, Rey Nann Mark Abaque. "Direct Detection of Aggregates in Turbid Colloidal Suspensions." Miami University / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=miami1439434385.

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Šedý, Jakub. "Turbo konvoluční a turbo blokové kódy." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2011. http://www.nusl.cz/ntk/nusl-219287.

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The aim is to explain the Turbo convolutional and block turbo codes and decoding the secure message. The practical part focuses on the design of a demonstration program in Matlab. The work is divided into four parts. The first two deal with theoretical analysis of coding and decoding. The third section contains a description created a demonstration program that allows you to navigate the process of encoding and decoding. The fourth is devoted to simulation and performance of turbo codes.
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Bücher zum Thema "TurboID"

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Turbide, Rachel. Répertoire des familles Turbide et Turbis: Descendantes de Dominique Dithurbide et Marie-Anne Boudrot. Editions R. Turbide, 1996.

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Bermúdez, Iván Cerdán. Turbio. Huerga & Fierro Editores, 2014.

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Plomer, William. Turbott Wolfe. Modern Library, 2003.

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Plomer, William. Turbott Wolfe. Oxford University Press, 1985.

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Plomer, William. Turbott Wolfe. Harcourt Brace Jovanovich, 1987.

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Kotli͡ar, M. M. Turboty ahronoma. "Tavrii͡a", 1986.

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Livmar, Pabsi. Teoremas turbios. EDP University of Puerto Rico, Inc., 2018.

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Gu, Min, Xiaosong Gan, and Xiaoyuan Deng. Microscopic Imaging Through Turbid Media. Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-662-46397-0.

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Rojas, Gonzalo. Río turbio. Hiperión, 1996.

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Rojas, Gonzalo. Río turbio. El Kultrún/Barba de Palo, 1996.

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Buchteile zum Thema "TurboID"

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Gryffroy, Lore, Joren De Ryck, Veronique Jonckheere, Sofie Goormachtig, Alain Goossens, and Petra Van Damme. "Cataloguing Protein Complexes In Planta Using TurboID-Catalyzed Proximity Labeling." In Methods in Molecular Biology. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3327-4_26.

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Artan, Murat, and Mario de Bono. "Proteomic Analysis of C. Elegans Neurons Using TurboID-Based Proximity Labeling." In Neuromethods. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2321-3_15.

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Lin, Danni, Jiayi Kuang, and Caiji Gao. "Identification of Neighboring Proteins of Endosomal Regulators by Using TurboID-Based Proximity Labeling." In Methods in Molecular Biology. Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-4059-3_11.

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Li, Yuanyuan, Yongliang Zhang, and Savithramma P. Dinesh-Kumar. "TurboID-Based Proximity Labeling: A Method to Decipher Protein–Protein Interactions in Plants." In Methods in Molecular Biology. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3485-1_19.

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Karunadasa, Sumudu S., TaraBryn S. Grismer, Wenxuan Zhai, Andres V. Reyes, and Shou-Ling Xu. "Detection and Quantification of Biotinylated Proteins for TurboID-Based Proximity Labeling Mass Spectrometry in Arabidopsis." In Methods in Molecular Biology. Springer US, 2025. https://doi.org/10.1007/978-1-0716-4694-6_8.

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Karunadasa, Sumudu S., TaraBryn S. Grismer, Andres V. Reyes, Wenxuan Zhai, and Shou-Ling Xu. "Detection and Quantification of Biotinylated Sites for TurboID-Based Proximity Labeling Mass Spectrometry in Arabidopsis." In Methods in Molecular Biology. Springer US, 2025. https://doi.org/10.1007/978-1-0716-4694-6_9.

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De Backer, Jonas, Lindsy De Veirman, and Inge De Clercq. "Characterization of the Protein Interactome of Membrane-Bound Transcription Factors Using TurboID-Based Proximity Labeling in Planta." In Methods in Molecular Biology. Springer US, 2025. https://doi.org/10.1007/978-1-0716-4694-6_11.

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Gooch, Jan W. "Turbid." In Encyclopedic Dictionary of Polymers. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_12215.

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Person-Le Ruyet, Jeannine. "Turbot Culture." In Practical Flatfish Culture and Stock Enhancement. Wiley-Blackwell, 2010. http://dx.doi.org/10.1002/9780813810997.ch7.

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Richards-Kortum, Rebecca. "Fluorescence Spectroscopy of Turbid Media." In Optical-Thermal Response of Laser-Irradiated Tissue. Springer US, 1995. http://dx.doi.org/10.1007/978-1-4757-6092-7_20.

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Konferenzberichte zum Thema "TurboID"

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Zubiaga Elordieta, Ana Mari, Jone Mitxelena Sánchez, James D. Sutherland, and Ekaitz Madariaga Carrero. "Gertuko biotinilazioa E2F7 transkripzio faktorearen interaktoma deskribatzeko estrategia gisa: TurboID sistemaren garapena." In V. Ikergazte. Nazioarteko ikerketa euskaraz. UEU arg, 2023. http://dx.doi.org/10.26876/ikergazte.v.04.15.

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Mair, Andrea. "Bringing the ‘PL’ to Plants - Proximity labeling with TurboID as a new tool to study protein complexes and cellular proteomes in plants." In ASPB PLANT BIOLOGY 2020. ASPB, 2020. http://dx.doi.org/10.46678/pb.20.989688.

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Taylor, Alexander H., Pavan Naik, Simon Nibler, and Nisar Al-Hasan. "Optimization of Variable Geometry Turbine Electric Turbocharger for a Heavy-Duty, On-Highway Fuel Cell." In ASME Turbo Expo 2023: Turbomachinery Technical Conference and Exposition. American Society of Mechanical Engineers, 2023. http://dx.doi.org/10.1115/gt2023-101224.

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Abstract An air compressor is a necessary component on fuel cell electric generators as fuel cells do not pull in air without an external intervention. There are many fuel cell air compression options, each with their own benefits and drawbacks, such as roots-style screw compressors, radial e-compressors, e-turbos with wastegate, and e-turbos with a variable geometry turbine (VTG). Turbocharger turbine wheels for internal combustion engine applications are optimized to maximize exhaust gas enthalpy extraction while limiting centrifugal forces in the blades. In the presence of high temperature
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Jezequel, M. "Turbo4: a high bit-rate chip for turbo code encoding and decoding." In IEE Colloquium. Turbo Codes in Digital Broadcasting - Could it Double Capacity? IEE, 1999. http://dx.doi.org/10.1049/ic:19990784.

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Han, Wook-Shin, Jinsoo Lee, and Jeong-Hoon Lee. "Turboiso." In the 2013 international conference. ACM Press, 2013. http://dx.doi.org/10.1145/2463676.2465300.

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Cizmar, Tomas. "Imaging through Turbid Media." In Computational Optical Sensing and Imaging. OSA, 2014. http://dx.doi.org/10.1364/cosi.2014.cw1c.2.

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Wood, Michael F. G., Nirmalya Ghosh, Marika A. Wallenburg, et al. "Turbid polarimetry for tissue characterization." In European Conference on Biomedical Optics. OSA, 2009. http://dx.doi.org/10.1364/ecbo.2009.7371_06.

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Wood, Michael F. G., Nirmalya Ghosh, Marika A. Wallenburg, et al. "Turbid polarimetry for tissue characterization." In European Conferences on Biomedical Optics, edited by Christian D. Depeursinge and I. Alex Vitkin. SPIE, 2009. http://dx.doi.org/10.1117/12.831744.

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Wang, Lihong V., and Qimin Shen. "Sonoluminescence tomography of turbid media." In BiOS '99 International Biomedical Optics Symposium, edited by Britton Chance, Robert R. Alfano, and Bruce J. Tromberg. SPIE, 1999. http://dx.doi.org/10.1117/12.356828.

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Dayton, A. L., and S. A. Prahl. "Turbid-polyurethane phantom for microscopy." In Biomedical Optics (BiOS) 2008, edited by Robert J. Nordstrom. SPIE, 2008. http://dx.doi.org/10.1117/12.764010.

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Berichte der Organisationen zum Thema "TurboID"

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Jonkman, B. J., and M. L. ,. Jr Buhl. TurbSim User's Guide. Office of Scientific and Technical Information (OSTI), 2006. http://dx.doi.org/10.2172/891594.

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Jonkman, B. J., and M. L. ,. Jr Buhl. TurbSim User's Guide. Office of Scientific and Technical Information (OSTI), 2005. http://dx.doi.org/10.2172/15020326.

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Farr, Norman, Emmanuel Boss, Paul S. Hill, et al. Optical Data Transmission in a Turbid Environment. Defense Technical Information Center, 2011. http://dx.doi.org/10.21236/ada557234.

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Milligan, Timothy G. Evolution of Particle Size in Turbid Discharge Plumes. Defense Technical Information Center, 1999. http://dx.doi.org/10.21236/ada613280.

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Hill, Paul S., and Timothy G. Milligan. Evolution of Particle Size in Turbid Discharge Plumes. Defense Technical Information Center, 1998. http://dx.doi.org/10.21236/ada537161.

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Hill, Paul S., and Timothy G. Milligan. Evolution of Particle Size in Turbid Discharge Plumes. Defense Technical Information Center, 1997. http://dx.doi.org/10.21236/ada629421.

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Hill, Paul S. Evolution of particle size in turbid discharge plumes. Defense Technical Information Center, 1999. http://dx.doi.org/10.21236/ada630881.

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Jonkman, B. J. Turbsim User's Guide: Version 1.50. Office of Scientific and Technical Information (OSTI), 2009. http://dx.doi.org/10.2172/965520.

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Phillips, Lee, and David Fyfe. TURBID: A Routine for Generating Random Turbulent Inflow Data. Defense Technical Information Center, 2011. http://dx.doi.org/10.21236/ada552556.

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10

Carder, Kendall L., and David K. Costello. Optical Variability and Bottom Classification in Turbid Waters: Phase II. Defense Technical Information Center, 2002. http://dx.doi.org/10.21236/ada628344.

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