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Zeitschriftenartikel zum Thema „Vitrification process“

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Veröffentlicht am 25. Mai 2024

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1

Faltus, Milos, Alois Bilavcik, Stacy Denise Hammond Hammond, and Jiri Zamecnik. "Vitrification process control by DSC." Cryobiology 109 (December 2022): 23–24. http://dx.doi.org/10.1016/j.cryobiol.2022.11.074.

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2

Odagaki, Takashi, and Akira Yoshimori. "Localization transition in the vitrification process." Physica B: Condensed Matter 296, no. 1-3 (2001): 174–79. http://dx.doi.org/10.1016/s0921-4526(00)00796-1.

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3

Short, Rick, Nick Gribble, Edward Turner, and Andrew D. Riley. "Using the Vitrification Test Rig for Process Improvements on the Waste Vitrification Plants." Advances in Science and Technology 73 (October 2010): 176–82. http://dx.doi.org/10.4028/www.scientific.net/ast.73.176.

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The Vitrification Test Rig (VTR) is a full scale non-active waste vitrification plant (WVP), that replicates the lines used for immobilising highly active reprocessing waste at Sellafield in the UK. In the high level waste (HLW) vitrification process, liquid HLW is dried in a rotating tube furnace then mixed with an alkali borosilicate glass frit. This mixture is heated to form a homogeneous product glass that is poured, cooled and stored in steel canisters. The primary function of the VTR is to trial and develop methods to increase the efficiency of high level waste processing at the active W

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4

Romero, M., and J. M. Rincón. "El proceso de vitrificación/cristalización controlada aplicado al reciclado de residuos industriales inorgánicos." Boletín de la Sociedad Española de Cerámica y Vidrio 39, no. 1 (2000): 155–63. http://dx.doi.org/10.3989/cyv.2000.v39.i1.884.

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5

Peymani, R., S. Najmabadi, H. Badrzadeh, T. M. Macaso, Z. Azadbadi, and A. Ahmady. "Comparison of two vitrification solutions on the outcome of vitrification/thaw process in a closed vitrification system, V-Kim." Fertility and Sterility 90 (September 2008): S286—S287. http://dx.doi.org/10.1016/j.fertnstert.2008.07.1105.

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6

F. N. C, Anyaegbunam. "Hazardous Waste Vitrification by Plasma Gasification Process." IOSR Journal of Environmental Science, Toxicology and Food Technology 8, no. 3 (2014): 15–19. http://dx.doi.org/10.9790/2402-08311519.

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7

Schug, Brett W., and Matthew J. Realff. "Analysis of waste vitrification product-process systems." Computers & Chemical Engineering 22, no. 6 (1998): 789–800. http://dx.doi.org/10.1016/s0098-1354(98)80002-1.

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8

Masrat-Un-Nisa, Asloob Ahmad Malik, Khursheed Ahmad Sofi, Arjuma Khatun, and Nahida Yousuf. "Recent Advancements in Vitrification Cryodevices for Gamete and Gonadal Tissue." Cryoletters 43, no. 3 (2022): 129–39. http://dx.doi.org/10.54680/fr22310110112.

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Cryopreservation of gametes and gonadal tissue is nowadays primarily accomplished through vitrification. Variables such as cooling rate, viscosity and volume of vitrification solution are critical in gamete vitrification. In addition, sample size and stepwise exposure are also crucial for gonadal tissue vitrification. Recently a class of cryodevices has been developed to reduce the volume of vitrification solution so as to achieve higher cooling rates. Vitrification devices are classified as "open" or "closed" depending on whether the medium comes into direct contact with liquid nitrogen durin

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Widjiati, Widjiati, Epy Muhammad Luqman, and Portia Sumarsono. "Comparison of Morula and Blastula Embryo Vitrification by Using Cryoprotectant Ethylene Glycol, Propanediol, DMSO and Insulin Transferrin Selenium." KnE Life Sciences 3, no. 6 (2017): 205. http://dx.doi.org/10.18502/kls.v3i6.1129.

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Vitrification is freezing method with low temperature (-196ºC) using high concentrations of cryoprotectants with a view to preventing the formation of ice crystals that can damage cells and decrease the viability of the embryo blastomeres. Embryos post warming which has low viability when transferred to a recipient will decrease the pregnancy rate. Intracellular cryoprotectants used in vitrification is ethylene glycol, propanediol, or DMSO. The third type of cryoprotectants has different capacities to protect the morula and blastocyst stage embryos. This study aims to decide the exact type of

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10

Odagaki, Takashi. "Non-Ergodicity and Non-Gaussianity in Vitrification Process." Progress of Theoretical Physics Supplement 126 (1997): 9–12. http://dx.doi.org/10.1143/ptps.126.9.

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11

Odagaki, T. "Non-Ergodicity and Non-Gaussianity in Vitrification Process." Progress of Theoretical Physics Supplement 126 (May 16, 2013): 9–12. http://dx.doi.org/10.1143/ptp.126.9.

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12

Blatter, A., N. Koch, and U. Kambli. "The process of spontaneous vitrification evaluated by calorimetry." Journal of the Less Common Metals 145 (December 1988): 81–88. http://dx.doi.org/10.1016/0022-5088(88)90264-0.

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13

Chang, Ching-Chien, Daniel B. Shapiro, and Zsolt Peter Nagy. "The effects of vitrification on oocyte quality." Biology of Reproduction 106, no. 2 (2021): 316–27. http://dx.doi.org/10.1093/biolre/ioab239.

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Abstract Vitrification, is an ultra-rapid, manual cooling process that produces glass-like (ice crystal-free) solidification. Water is prevented from forming intercellular and intracellular ice crystals during cooling as a result of oocyte dehydration and the use of highly concentrated cryoprotectant. Though oocytes can be cryopreserved without ice crystal formation through vitrification, it is still not clear whether the process of vitrification causes any negative impact (temperature change/chilling effect, osmotic stress, cryoprotectant toxicity, and/or phase transitions) on oocyte quality,

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Yang, Yanzhou, Jie Chen, Hao Wu, et al. "The Increased Expression of Connexin and VEGF in Mouse Ovarian Tissue Vitrification by Follicle Stimulating Hormone." BioMed Research International 2015 (2015): 1–13. http://dx.doi.org/10.1155/2015/397264.

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Ovarian follicular damages were caused by cryoinjury during the process of ovarian vitrification and ischemia/reperfusion during the process of ovarian transplantation. And appropriate FSH plays an important role in antiapoptosis during ovarian follicle development. Therefore, in this study, 0.3 IU/mL FSH was administered into medium during mouse ovarian cryopreservation by vitrification to ascertain the function of FSH on ovarian vitrification and avascular transplantation. The results suggested that the expressions of Cx37, Cx43, apoptotic molecular caspase-3, and angiogenesis molecular VEGF

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Campbell, Lia H., and Kelvin G. M. Brockbank. "Development of a Vitrification Preservation Process for Bioengineered Epithelial Constructs." Cells 11, no. 7 (2022): 1115. http://dx.doi.org/10.3390/cells11071115.

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The demand for human bioengineered tissue constructs is growing in response to the worldwide movement away from the use of animals for testing of new chemicals, drug screening and household products. Presently, constructs are manufactured and delivered just in time, resulting in delays and high costs of manufacturing. Cryopreservation and banking would speed up delivery times and permit cost reduction due to larger scale manufacturing. Our objective in these studies was development of ice-free vitrification formulations and protocols using human bioengineered epithelial constructs that could b

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Liu, Yue, Andy Lin, Terrence R. Tiersch, and William Todd Monroe. "A 3D Printed Vitrification Device for Storage in Cryopreservation Vials." Applied Sciences 11, no. 17 (2021): 7977. http://dx.doi.org/10.3390/app11177977.

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Sperm cryopreservation by vitrification is a promising approach for small-bodied animals such as zebrafish (Danio rerio). However, most vitrification tools adopted in aquatic research were initially designed for applications other than sperm (such as human embryo freezing) and, thus, pose challenges for adoption to sperm vitrification. Three-dimensional (3D) printing combined with open hardware sharing is an emerging strategy to address challenges in the development of cryopreservation tools. The goal of this study was to develop a 3D printed Vitrification Device for Cryo-Vials (VDCV) that can

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Trifunović, Vanja. "Vitrification as a method of soil remediation." Zastita materijala 62, no. 3 (2021): 166–79. http://dx.doi.org/10.5937/zasmat2103166t.

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Various types of contaminated soil and hazardous waste that have a negative impact on the environment and human health can be treated with the vitrification process. This process is based on thermal treatment of contaminated soil or waste at high temperatures, with the addition of additives, whereby the soil/waste melts and a stable glass is formed. The resulting glass and glass-ceramic products have good mechanical resistance, chemically are resistant and immobilize contaminants, thus preventing their further negative impact on the environment. This paper presents a literature review of the v

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Lan, Tianyang, Kang Zhang, Feifei Lin, et al. "Effects of MICU1-Mediated Mitochondrial Calcium Uptake on Energy Metabolism and Quality of Vitrified-Thawed Mouse Metaphase II Oocytes." International Journal of Molecular Sciences 23, no. 15 (2022): 8629. http://dx.doi.org/10.3390/ijms23158629.

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Background: Oocyte vitrification has been widely used in the treatment of infertility and fertility preservation. However, vitrification-induced mitochondrial damage adversely affects oocyte development. Several studies have reported that mitochondrial calcium uptake protein 1 (MICU1) regulates the uptake of mitochondrial calcium by the mitochondrial calcium uniporter (MCU) and subsequently controls aerobic metabolism and oxidative stress in mitochondria, but research considering oocytes remains unreported. We evaluated whether the addition of MICU1 modulators enhances mitochondrial activity,

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Marco-Jiménez, F., L. Casares-Crespo, and J. S. Vicente. "Porcine oocyte vitrification in optimized low toxicity solution with open pulled straws." Zygote 22, no. 2 (2012): 204–12. http://dx.doi.org/10.1017/s0967199412000524.

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SummaryOne of the greatest challenges for reproductive cryobiologists today is to develop an efficient cryopreservation method for human and domestic animal oocytes. The objective of the present study was to optimize a low toxicity solution called VM3 to vitrify porcine oocytes using an open pulled straw (OPS) device and to evaluate the effects on viability, chromosomal organization and cortical granules distribution. Two experiments were conducted in this study. Firstly, we determined the minimum concentration of cryoprotectant present in the VM3 solution required (7.6 M) for vitrification us

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Deptula, Andrzej, Magdalena Milkowska, Wieslawa Lada, et al. "Vitrification of Nuclear Wastes by Complex Sol-Gel Process." Advanced Materials Research 518-523 (May 2012): 3216–20. http://dx.doi.org/10.4028/www.scientific.net/amr.518-523.3216.

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For synthesis of silica glasses designed to contain high-level nuclear wastes，a patented complex sol-gel process has been used. Surrogates of the nuclear waste elements Cs, Sr, Co, and Nd (generically denoted Me) were used. Gels in the forms of powders and sintered compacts were prepared by hydrolysis and polycondensation of tetraethoxide/Me nitrate solutions, which contained ascorbic acid as a catalyst. Transformation to final products was studied by thermogravimetric analysis, infrared spectroscopy, and X-ray diffraction. Preliminary testing of Me leaching was also completed in water. Most o

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Langerman, M. A., and R. J. MacKinnon. "Scaling considerations for modelling the in situ vitrification process." Applied Mathematical Modelling 15, no. 10 (1991): 542–49. http://dx.doi.org/10.1016/0307-904x(91)90056-u.

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22

Hoang, V. V. "Atomic mechanism of vitrification process in simple monatomic nanoparticles *." European Physical Journal D 61, no. 3 (2011): 627–35. http://dx.doi.org/10.1140/epjd/e2011-10651-1.

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23

Ševelová, Helena, and Miloslava Lopatářová. "Closed system for bovine oocyte vitrification." Acta Veterinaria Brno 81, no. 2 (2012): 201–6. http://dx.doi.org/10.2754/avb201281020201.

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The aim of our study was to develop a vitrification carrier for bovine oocyte cryopreservation. The carrier was to be cheap enough, elementary in its construction and meet contemporary requirements for a safe closed system. In a closed system, a cell is prevented from direct exposure to liquid nitrogen, thus minimizing the risk of cross-contamination. Furthermore, two questions regarding the proper vitrification technique were resolved: if it is necessary to partially denude the oocytes before the vitrification process or whether intact cumulus oocyte complexes should be frozen; and if it is m

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Madrid Gaviria, Stephania, Sergio A. Morado, Albeiro López Herrera, et al. "Resveratrol supplementation promotes recovery of lower oxidative metabolism after vitrification and warming of in vitro-produced bovine embryos." Reproduction, Fertility and Development 31, no. 3 (2019): 521. http://dx.doi.org/10.1071/rd18216.

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Although vitrification is the current method of choice for oocyte and embryo cryopreservation, it may have detrimental effects on reduction–oxidation status and mitochondrial activity. The aim of this study was to evaluate the effect of supplementing invitro culture (IVC) media and/or vitrification solutions with the antioxidant resveratrol on active mitochondria, mitochondrial superoxide production and lipid peroxidation. Abattoir-derived oocytes were matured and fertilised invitro using standard procedures. Following IVF (21h later), zygotes were cultured in IVC medium supplemented with 0 or

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Nowok, Andrzej, Piotr Kuś, Mateusz Dulski, et al. "Role of intermolecular interactions and conformational changes in the polymorphism and vitrification process of 2,2′′-bis-substituted para-terphenyls." CrystEngComm 22, no. 18 (2020): 3164–78. http://dx.doi.org/10.1039/d0ce00351d.

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26

Lin, Chiahsin, Wen-Chung Hsieh, Kanokpron Loeslakwiboon, Cheng-Liang Huang, Ting-Chun Chen, and Sujune Tsai. "Refined Techniques for Enabling Long-Term Cryo-Repository Using Vitrification and Laser Warming." Bioengineering 10, no. 5 (2023): 605. http://dx.doi.org/10.3390/bioengineering10050605.

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Vitrification and ultrarapid laser warming are crucial for the cryopreservation of animal embryos, oocytes, and other cells of medicinal, genetic, and agricultural value. In the present study, we focused on alignment and bonding techniques for a special cryojig that combines a jig tool and jig holder into one piece. This novel cryojig was used to obtain a high laser accuracy of 95% and a successful rewarming rate of 62%. The experimental results indicated that our refined device improved laser accuracy in the warming process after long-term cryo-storage through vitrification. We anticipate tha

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W Lestari, Silvia, Nurin N. Fitriyah, and Ria Margiana. "An Update of Oocyte Vitrification: A Modification of Sucrose and Trehalose as Extracellular Cryoprotectant." Biomedical and Pharmacology Journal 11, no. 1 (2018): 209–14. http://dx.doi.org/10.13005/bpj/1365.

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As well as the development of assisted reproductive technology (ART), as the current treatment of woman who failed in achieving pregnancy, the development of an advance vitrification method also grows rapidly. The successful of oocyte vitrification depends on the type and the concentration of cryoprotectant. This study was addressed to elaborate empirical evidence and recent studies of sucrose and trehalose as an extracellular CPA with the aim of achieving the success of oocyte vitrification. Several researchers in agreement that trehalose, as extracellular cryoprotectant, also has a role as i

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Su, Fengmin, Yiming Fan, Chi Zhang, Yifan Wang, Yanyang Wang, and Benli Peng. "Vitrification by Transient Vacuum Flashing Spray Cooling of Liquid Nitrogen." Cryoletters 43, no. 3 (2022): 167–74. http://dx.doi.org/10.54680/fr22310110212.

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BACKGROUND: The transient vacuum flashing spray cooling of liquid nitrogen (LN2 ) on a microstructured surface can provide ultra-fast cooling rate and may improve cell survival rates. OBJECTIVE: To utilize flashing spray cooling of LN2 instead of film boiling to improve further cell vitrification. METHOD: This study analyzed the effects of the three key parameters (flow rate of liquid nitrogen, ambient pressure, and spray distance) on the cooling process by experimentation. RESULTS: The experimental results showed that the vacuum flashing spray cooling of LN2 can gain higher cooling rates than

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Roth, G., and S. Weisenburger. "Vitrification of high-level liquid waste: glass chemistry, process chemistry and process technology." Nuclear Engineering and Design 202, no. 2-3 (2000): 197–207. http://dx.doi.org/10.1016/s0029-5493(00)00358-7.

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Isachenko, V., I. Lapidus, E. Isachenko, et al. "Human ovarian tissue vitrification versus conventional freezing: morphological, endocrinological, and molecular biological evaluation." REPRODUCTION 138, no. 2 (2009): 319–27. http://dx.doi.org/10.1530/rep-09-0039.

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Cryopreservation as a process can be divided into two methods: conventional freezing and vitrification. The high effectiveness of vitrification in comparison with conventional freezing for human oocytes and embryos is shown, whereas data on human ovarian tissue are limited. The aim of this study was to compare the safety and effectiveness of conventional freezing and vitrification of human ovarian tissue. Ovarian tissue fragments from 15 patients were transported to the laboratory within 22–25 h in a special, isolated transport box that can maintain a stable temperature of between 5 and 8 °C f

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Fu, Xufeng, Yaping Yan, Shanshan Li, et al. "Vitrification of Rhesus Macaque Mesenchymal Stem Cells and the Effects on Global Gene Expression." Stem Cells International 2017 (2017): 1–14. http://dx.doi.org/10.1155/2017/3893691.

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Mesenchymal stem cells (MSCs) are one of the most promising adult stem cells for clinical application in a cell therapy. The development of large-scale cryopreservation techniques, such as vitrification, for MSCs is a prerequisite for clinical therapies. Dimethyl sulfoxide (DMSO) and ethylene glycol (EG) are two types of cryoprotectants widely used for cell vitrification. However, the effects of DMSO and EG on the biological characteristics and transcriptome profiles of MSCs after cryopreservation remain unknown. In the present study, the viability, immunophenotype of cell surface markers, pro

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Li, Yong, Xue Gang Liu, and Jin Chen. "The Application Research of Concentration & Denitration Technology in Advanced Reprocessing Process." Advanced Materials Research 560-561 (August 2012): 637–43. http://dx.doi.org/10.4028/www.scientific.net/amr.560-561.637.

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The proper management of spent fuel arising from nuclear power production is a key issue for the sustainable development of nuclear energy. While conventional reprocessing process, PUREX process, was successful to recover uranium and plutonium, in recent years some countries have turned to focus on advanced reprocessing process, which features of partitioning of minor actinides (MA) and long-lived fission products(LLFP). Most advanced reprocessing processes under development involve new extractants and additional extraction cycles. In China, TRPO extraction process has been developed to partit

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Nakahara, Keisuke. "Vitrification Process for Recovery of Phosphorus from Sewage Sludge Ash." RESOURCES PROCESSING 50, no. 2 (2003): 68–73. http://dx.doi.org/10.4144/rpsj1986.50.68.

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Kikuchi, Ryunosuke. "Vitrification Process for Treatment of Sewage Sludge and Incineration Ash." Journal of the Air & Waste Management Association 48, no. 11 (1998): 1112–15. http://dx.doi.org/10.1080/10473289.1998.10463766.

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Egorov, E. A., and V. V. Zhizhenkov. "Influence of Mechanical Vitrification on Flexible-Chain Polymer Drawing Process." International Journal of Polymeric Materials 22, no. 1-4 (1993): 41–47. http://dx.doi.org/10.1080/00914039308012056.

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Odagaki, T., J. Matsui, and M. Higuchi. "Dynamical characteristics of the vitrification process of an ideal system." Journal of Physics: Condensed Matter 10, no. 49 (1998): 11491–98. http://dx.doi.org/10.1088/0953-8984/10/49/035.

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Ranganathan, S., and P. Ramachandra Rao. "Thermodynamic modelling of the spontaneous vitrification process using transformation diagrams." Calphad 34, no. 4 (2010): 387–92. http://dx.doi.org/10.1016/j.calphad.2010.07.004.

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Iwaszko, Józef, Monika Zajemska, Anna Zawada, Stanisław Szwaja, and Anna Poskart. "Vitrification of environmentally harmful by-products from biomass torrefaction process." Journal of Cleaner Production 249 (March 2020): 119427. http://dx.doi.org/10.1016/j.jclepro.2019.119427.

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Yoshioka, M., S. Torata, J. Igarashi, T. Takahashi, and M. Horie. "Glass melter and process development for PNC Tokai vitrification facility." Waste Management 12, no. 1 (1992): 7–16. http://dx.doi.org/10.1016/0956-053x(92)90003-2.

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Faizah, Zakiyatul, R. Haryanto Aswin, Hamdani Lunardhi, and Widjiati Widjiati. "Hyaluronan Expression on Vitrified Oocytes Before and After In Vitro Maturation (EKSPRESI HYALURONAN PADA OOSIT YANG DIVITRIFIKASI SEBELUM DAN SESUDAH MATURASI IN VITRO)." Jurnal Veteriner 19, no. 1 (2018): 71. http://dx.doi.org/10.19087/jveteriner.2018.19.1.71.

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Oocyte vitrification is a major challenge in assisted reproductive technology. Oocyte vitrification with cumulus cells provide benefits in the process of maturation and fertilization. Vitrification leads to rapid temperature changes, therefore the decreasing in temperature could damage the cells even when the morphology was normal. Vitrification of mature oocytes is common because of its low sensitiveness towards low temperatures than immature oocytes. The aim of the research was to compare the effect of vitrification before and after in vitro maturation to the expression of hyaluronan. Matura

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Sayenko, Sergii, Volodymyr Shkuropatenko, Yevhenii Svitlychnyi, et al. "Vitrification of a Simulator of Vat Residues from Liquid Radioactive Waste." East European Journal of Physics, no. 1 (March 2, 2023): 94–101. http://dx.doi.org/10.26565/2312-4334-2023-1-11.

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The study on the posibility of the use of the optimal glass compositions for vitrification of an imitator of vat residues of liquid radioactive waste from nuclear power plants with VVER-1000 reactors was carried out. The main process parameters such as vitrification temperature, strength, corrosion resistance, absence of crystalline phases, minimization of glass-forming additives and inclusion the maximum amount of waste were analyzed. It has been established that the melting temperature of lead-borosilicate glass matrices was 1150 °C, which satisfies the requirements for vitrification of low-

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Wang, Mengying, Plamen Todorov, Wanxue Wang, et al. "Cryoprotectants-Free Vitrification and Conventional Freezing of Human Spermatozoa: A Comparative Transcript Profiling." International Journal of Molecular Sciences 23, no. 6 (2022): 3047. http://dx.doi.org/10.3390/ijms23063047.

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Introduction: Spermatozoa cryopreservation is an important technique to preserve fertility for males. This study aimed at exploring the stability of epigenetics information in human spermatozoa, manipulated by two different technologies, freezing and vitrification. Methods: Spermatozoa samples were distributed into three groups: 1. Fresh spermatozoa (control group), 2. Frozen spermatozoa, 3. Vitrified spermatozoa. Epigenetic differences of fresh and cryopreserved spermatozoa were evaluated using high-throughput RNA sequencing. Results: Differentially expressed genes (DEGs) in frozen (1103 gene

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Matrosov, Andrey, Arkady Soloviev, Elena Ponomareva, et al. "Finite Element Modeling of Crystallization with Temperature Jump to Improve Cryopreservation of Fish Germ Cells." Processes 12, no. 2 (2024): 413. http://dx.doi.org/10.3390/pr12020413.

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This article is devoted to the further development of a viable technology for low-temperature cryopreservation of reproductive cells of sturgeon fish using acoustic–mechanical fields and intelligent control of the freezing process. Before vitrification begins, the piezoactuator acts on a mixture of cryoprotectant and reproductive cells. This promotes intensive mixing of the cryoprotector and its diffusion through the cell membrane. When vitrification is carried out directly, a phase transition phenomenon is observed, accompanied by crystal formation. This article presents a new mathematical mo

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Do, V. H., S. Catt, J. E. Kinder, S. Walton, and A. W. Taylor-Robinson. "Vitrification of in vitro-derived bovine embryos: targeting enhancement of quality by refining technology and standardising procedures." Reproduction, Fertility and Development 31, no. 5 (2019): 837. http://dx.doi.org/10.1071/rd18352.

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Bovine invitro fertilisation technology has been widely exploited in commercial settings. The majority of invitro-derived cattle embryos are transferred into recipient cows as recently collected (i.e. ‘fresh’) embryos due to the lack of a reliable cryopreservation method that results in favourable pregnancy rates following transfer of thawed embryos. This is a primary reason for the poor industry uptake of this extreme temperature freezing process. Numerous investigations into vitrification have revealed the importance of rapid cooling and warming rates, enhancing embryo viability after cryopr

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Vajta, Gábor. "Vitrification in human and domestic animal embryology: work in progress." Reproduction, Fertility and Development 25, no. 5 (2013): 719. http://dx.doi.org/10.1071/rd12118.

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According to the analysis of papers published in major international journals, rapidly increasing application of vitrification is one of the greatest achievements in domestic animal and especially human embryology during the first decade of our century. This review highlights factors supporting or hampering this progress, summarises results achieved with vitrification and outlines future tasks to fully exploit the benefits of this amazing approach that has changed or will change many aspects of laboratory (and also clinical) embryology. Supporting factors include the simplicity, cost efficienc

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de Mello, Fernanda, Daniel Jaen Alonso, Natália Pires Vieira Morais de Faria, et al. "Alterations in Gene Expression and the Fatty Acid Profile Impact but Do Not Compromise the In Vitro Maturation of Zebrafish (Danio rerio) Stage III Ovarian Follicles after Cryopreservation." Animals 13, no. 22 (2023): 3563. http://dx.doi.org/10.3390/ani13223563.

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The vitrification of ovarian follicles is a strategic tool that may contribute to advances in aquaculture and the conservation of many important species. Despite the difficulties inherent to the cryopreservation of oocytes, some successful protocols have been developed for different species, but little is known about the capacity of oocytes to develop after thawing. Therefore, the profiles of the reproductive pathway genes and fatty acid membrane composition during the initial stages of development were analyzed in fresh ovarian follicles and follicles after the vitrification process. There we

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Yun, Jong-Il, Reinhardt Klenze, and Jae-Il Kim. "Laser-Induced Breakdown Spectroscopy for the On-Line Multielement Analysis of Highly Radioactive Glass Melt Simulants. Part II: Analyses of Molten Glass Samples." Applied Spectroscopy 56, no. 7 (2002): 852–58. http://dx.doi.org/10.1366/000370202760171518.

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The paper presents the application of laser-induced breakdown spectroscopy (LIBS) for the on-line multielement analyses of glass melts in a vitrification process of high level liquid waste (HLLW). The third harmonic pulse of an Nd:YAG laser is used for the generation of plasma on the molten glass surface and the plasma emission is monitored by an echelle spectrometer with an intensified charge-coupled device (ICCD), which simultaneously covers the wavelength range from 200 to 780 nm. Twelve different reference HLLW glass melts with a complex composition of about 27 chemical elements are simula

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Fuller, D., J. Herrick, J. Graham, and J. Barfield. "42 Vitrification of invitro-produced feline embryos." Reproduction, Fertility and Development 32, no. 2 (2020): 146. http://dx.doi.org/10.1071/rdv32n2ab42.

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Preservation of feline embryos is useful in propagating endangered species, preserving valuable genetics, and supporting biomedical research. Although a wide variety of cryoprotectants (CP) and protocols are successfully used for vitrification of invitro-produced (IVP) embryos, there are often species-specific differences in viability of embryos post-warming. The purpose of this study was to evaluate the viability of IVP feline embryos after vitrification using two common CPs, propanediol (PrOH) or ethylene glycol (EG). Embryos were produced with oocytes and frozen-thawed epididymal sperm coll

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Gutierrez-Castillo, Emilio, Hao Ming, Brittany Foster, et al. "Effect of vitrification on global gene expression dynamics of bovine elongating embryos." Reproduction, Fertility and Development 33, no. 5 (2021): 338. http://dx.doi.org/10.1071/rd20285.

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Embryo vitrification involves exposure to high concentrations of cryoprotectants and osmotic stress during cooling and warming in the cryopreservation process. Many of these factors can potentially affect gene expression. In this study, invitro-produced bovine embryos at the blastocyst stage were subjected to vitrification. Four recipients each were used for transferring non-vitrified (n=80) and vitrified (n=80) embryos. A total of 12 non-vitrified and 9 vitrified viable day-14 (D14) embryos were recovered by uterine flushing. RNA-seq analysis of the whole embryo or isolated trophectoderm (TE)

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Zakošek Pipan, Maja, Margret L. Casal, Nataša Šterbenc, Irma Virant Klun, and Janko Mrkun. "Vitrification Using Soy Lecithin and Sucrose: A New Way to Store the Sperm for the Preservation of Canine Reproductive Function." Animals 10, no. 4 (2020): 653. http://dx.doi.org/10.3390/ani10040653.

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A challenge in freezing semen for short and long-term availability is avoiding damage to intact spermatozoa caused by the freezing process. Vitrification protocols provide better results through less manipulation of semen and shorter freezing time compared to slow freezing techniques. Our research was aimed at improving vitrification methods for canine semen. Semen quality was determined in 20 ejaculates after collection. Each ejaculate was divided into eight aliquots, each with a different extender. The control extender contained TRIS, citric acid, fructose, and antibiotics. Soy lecithin and

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