Thematische Bibliographien / Yellow press

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Auswahl der wissenschaftlichen Literatur zum Thema „Yellow press“

Autor: Grafiati

Veröffentlicht am 4. Juni 2021

Zuletzt aktualisiert am 30. Mai 2022

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Zeitschriftenartikel zum Thema "Yellow press"

Martínez-Salanova-Sánchez, Enrique. „The yellow press“. Comunicar 15, Nr. 30 (01.03.2008): 102–5. http://dx.doi.org/10.3916/c30-2008-02-001.

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Lopez, Lori Kido. „The Yellow Press“. Journal of Communication Inquiry 35, Nr. 3 (23.06.2011): 235–51. http://dx.doi.org/10.1177/0196859911412377.

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ABDUAZIMOV, OQILJON. „THEORY OF “TANAZIAN PRESS”“. Main Issues Of Pedagogy And Psychology 3, Nr. 3 (20.12.2013): 173–78. http://dx.doi.org/10.24234/miopap.v3i3.321.

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In the article following issues are explained by the researcher encouraging lots of scholars ideas, such as: the influence of “tanazian press” to the press activity, appearing eroding process in the society as a result of “tanazian press”, two new outlooks “wrecking ship”, “turned upside–down boat”, Jalaliddin Rumi’s outlooks about different suppositions can be met in the society, responsibility of journalists, “yellow press” and its bad activities, spreading unethical materials, as well as pornography, positively illumination of gay and lesbian marriages, aggressive crimes, activity of pimping, sexual attack, prostitution, abortion, cloning technologies by foreign journalists, rising of the world population getting old in some countries, their bad influences and etc.

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Noor, Muhamad Mulki Mulyadi, und Susanto Zuhdi. „Conflict in Private Land: The Role of “Yellow Journalism” in the Turmoil of Batu Ceper, Tangerang 1934“. Indonesian Historical Studies 4, Nr. 2 (01.12.2020): 113–27. http://dx.doi.org/10.14710/ihis.v4i2.8875.

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This article discusses the conflict and social unrest in the Batu Ceper private lands. The events in Batu Ceper was an example of anti-extortion movement erupted due to the Cuke and Kompenian problems against the background of the socio-economic issuessince the late 19th century. This study identifies “yellow journalism” concept which succeeded in uplifting the Batu Ceper event with a bombastic and sensational headline in the form of an exciting debate between the newspapers of the landlord’s defender (the white press) and the peasant advocates (press Indonesier). The victory of the white press in the court did not mean the end of potential chaos, because the anxiety which became the factor of chaos never faded away due to a mere court ruling. This article reflects the field of social history, in which the study uses mass media as its primary focus. It shows the characteristic of disruption in a historical perspective.

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Fanani, Fajriannoor. „Harian Kuning dan Media Sensasional Islam“. Jurnal The Messenger 4, Nr. 2 (18.01.2016): 1. http://dx.doi.org/10.26623/themessenger.v4i2.148.

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Yellow press or yellow journalism is types of journalism that have little concern to accuracy and impartiality. This kind of journalism usually used in many metropolitan newspaper that heavily sensationalized crime, sex, and metaphysical news. This kind of newspaper allegedly also found in some Islamic magazine with its own characteristic, in this case Sabili Magazine. These magazines have some unique journalism characteristic that could be compared to yellow journalism. This is including sensationalist, bombastic, and partisan. This writing tries to show how Sabili magazine used types of journalism that have similarities with yellow journalism.

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WOODARD, JAMES P. „Pages from a Yellow Press: Print Culture, Public Life and Political Genealogies in Modern Brazil“. Journal of Latin American Studies 46, Nr. 2 (29.04.2014): 353–79. http://dx.doi.org/10.1017/s0022216x14000030.

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AbstractAn examination of the Brazilian newspaper O Combate, this article accomplishes four goals. First, it defines the politics of a periodical long cited but little understood by historians. Second, it documents O Combate's place, alongside other ‘yellow press’ outlets, in the making of a ‘public sphere’ in São Paulo. Third, it situates the same publications' role in the bringing into being of a more commercial, publicity-driven press, which would shed the yellow press's radicalism and abet the collapse of the public sphere of its heyday. Fourth, it suggests that O Combate's radical republicanism was one fount of the democratic radicalism of the late 1920s and early 1930s, as well as of the regionally chauvinist constitutionalism of 1932–7. In this rare application of the ‘public sphere’ idea to twentieth-century Brazil, readers may also detect an account closer to Jürgen Habermas’ original formulation than that found in the historiography of nineteenth-century Spanish America.

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Rong, Huren, Jingyu Gu, Miren Rong, Hong Liu, Jiayao Zhang und Hao Dong. „Strength and Microscopic Damage Mechanism of Yellow Sandstone with Holes under Freezing and Thawing“. Advances in Civil Engineering 2020 (30.04.2020): 1–13. http://dx.doi.org/10.1155/2020/5921901.

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In order to study the damage characteristics of the yellow sandstone containing pores under the freeze-thaw cycle, the uniaxial compression test of saturated water-stained yellow sandstones with different freeze-thaw cycles was carried out by rock servo press, the microstructure was qualitatively analyzed by Zeiss 508 stereo microscope, and the microdamage mechanism was quantitatively studied by using specific surface area and pore size analyzer. The mechanism of weakening mechanical properties of single-hole yellow sandstone was expounded from the perspective of microstructure. The results show the following. (1) The number of freeze-thaw cycles and single-pore diameter have significant effects on the strength and elastic modulus of the yellow sandstone; the more the freeze-thaw cycles and the larger the pore size, the lower the strength of the yellow sandstone. (2) The damage modes of the yellow sandstone containing pores under the freeze-thaw cycle are divided into five types, and the yellow sandstone with pores is divided into two areas: the periphery of the hole and the distance from the hole; as the number of freeze-thaw cycles increases, different regions show different microscopic damage patterns. (3) The damage degree of yellow sandstone is different with freeze-thaw cycle and pore size. Freeze-thaw not only affects the mechanical properties of yellow sandstone but also accelerates the damage process of pores. (4) The damage of the yellow sandstone by freeze-thaw is logarithmic function, and the damage of the yellow sandstone is a power function. The damage equation of the yellow sandstone with pores under the freezing and thawing is a log-power function nonlinear change law and presents a good correlation.

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Hershatter, Gail. „Disquiet in the House of Gender“. Journal of Asian Studies 71, Nr. 4 (November 2012): 873–94. http://dx.doi.org/10.1017/s0021911812001180.

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When I was a child at the height of the Cold War, the press painted China in vivid primary colors—red menace, yellow peril, blue-clad masses. China was a back-burner story, however, compared to the Soviet threat, and my education through high school never touched upon it. A shift in public attention began with the 1971 China visit of the U.S. ping-pong team and Nixon's state visit in February 1972. Suddenly the mainstream U.S. press discovered beggar-free streets, healthy children, acupuncture anesthesia, and national optimism.

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Rongyi, Zhang, Tan Zhiqiong und Cheng Shanying. „First Report of Leaf Rot Caused by Fusarium oxysporum and Pythium aphanidermatum on Aechmea fasciata in Hainan Province, China“. Plant Disease 87, Nr. 5 (Mai 2003): 599. http://dx.doi.org/10.1094/pdis.2003.87.5.599a.

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Aechmea fasciata is a bromeliad that is propagated by tissue culture as an ornamental plant. A high percentage (25 to 55%) of 1- to 5-month-old seedlings were found decayed in nursery gardens that have been established in recent years in Hainan Province, People's Republic of China. There were two types of symptoms: (i) in yellow rot, the decay appears first in the new leaf apex as a water-soaked, yellow lesion, and the lesion spreads from the leaf apex to the leaf base until the whole leaf becomes water soaked and yellow; and (ii) in brown rot, decay occurs first at the base of older leaves. The lesion is water soaked but later becomes brown, and the lesions develop from leaf base to leaf apex and spread to adjacent leaves. Eventually the whole plant is rotted, but the decay remains brown. Both types of symptoms on A. fasciata have not been reported in China. Fusarium sp. was isolated from the yellow rot type lesions, and Pythium sp. was isolated from the brown rot type lesions. Isolates of Fusarium and Pythium spp. were inoculated on wounded and unwounded healthy plants. Yellow rot type and brown rot type lesions were observed on wounded host three days after inoculation with the respective pathogen. No lesions were observed on unwounded inoculated plants or on wounded and unwounded control plants. The same Fusarium and Pythium spp. were reisolated from the yellow and brown rot type lesions, respectively, in inoculated plants, thus fulfilling Koch's postulates. Colonies of the Fusarium sp. were white on potato dextrose agar, purple on rice medium, and first yellow, and then blue green in 4 days on selective carrot medium. On carnation media, microconidia were abundant, 1- to 2-celled, oval- to kidney-shaped, and 2.4 to 3.6 × 4.8 to 7.2 μm. Macroconidia were rare, sickle-shaped with attenuated apical cells and foot-shaped basal cells. Chlamydospores were also abundant, globose, formed singly or in pairs, and intercalary or on short lateral branches. This pathogen was identified as Fusarium oxysporum based on these characteristics (1,3). The Pythium sp. on potato dextrose agar was white and cottony. On selective carrot medium, hyphae were 3.6 to 7.5 μm in diameter. Sporangia had irregular swellings and were acrogenous. Oogonia were globose, colorless, acrogenous or intercalary, 14.8 to 27.6 μm in diameter. Antheridia were copulated with oogonia in its apex or lateral. Oospores were spherical, aplerotic, and 13.2 to 25.2 μm in diameter. This pathogen was identified as Pythium aphanidermatum based on these characters (2). To our knowledge, this is first report of leaf rot caused by F. oxysporum and P. aphanidermatum on A. fasciata in the People's Republic of China. References: (1) C. Booth. The Genus Fusarium. The Eastern Press, London, 1971. (2) W. Jingchao. Pages 30–32 in: Manual of Fungi Identification. The Shanghai Science Technology Press, 1979. (3) P. E. Nelson et al. Fusarium Species-An Illustrated Manual of Identification. The Pennsylvania State University Press University Park, 1976.

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Cremonte, Martín, und Valcheff García Fernando. „Carlos Astrada. Textos de juventud || Natascha Veldhorst. Van Gogh and Music“. Boletín de Estética, Nr. 56 (15.12.2021): 113–22. http://dx.doi.org/10.36446/be.2021.56.282.

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Carlos Astrada. Textos de juventud: de la revolución universitaria a la vanguardia filosófica 1916-1927, introducción y compilación por Natalia Bustelo y Lucas Domínguez Rubio. Buenos Aires: CeDInCI Editores, 2021, 449 páginas. Natascha Veldhorst. Van Gogh and Music. A Symphony in Blue and Yellow, trad. de Diane Webb. New Haven and London: Yale University Press, 2018, 175 páginas.

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Dissertationen zum Thema "Yellow press"

Сушкова, Олена Миколаївна, Елена Николаевна Сушкова, Olena Mykolaivna Sushkova und Д. Сапун. „Явище "жовтої преси": для журналістів - хліб, для читачів - видовище“. Thesis, Видавництво СумДУ, 2007. http://essuir.sumdu.edu.ua/handle/123456789/16885.

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У сучасній журналістиці практично всіх країн світу жовта преса за всієї своєї примітивності займає досить значне місце, виконуючи розважальну функцію. В Україні за радянських часів жовта преса не існувала з огляду на цензурні перешкоди, але сьогодні вона створена і досить швидко розповсюджується. При цитуванні документа, використовуйте посилання http://essuir.sumdu.edu.ua/handle/123456789/16885

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Bonfig, Audrey E. „The Hatfields and the McCoys: America’s Feud, as Portrayed in the New York, West Virginia, and Kentucky Press, 1888-1890“. Ohio University / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1460146154.

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Botha, Marzahn. „Die geelpers in Suid-Afrika : 'n analise van die Kaapse Son, Daily Voice en Daily Sun / M. Botha“. Thesis, North-West University, 2009. http://hdl.handle.net/10394/2649.

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Although the yellow press has been in existence in Great Britain and the USA since the start of the 19th century, it has only recently been introduced in the South African media environment. The mainstream press like Rapport and Beeld have showed certain characteristics of the yellow press for a while now, but rather present a combination of both trivial and more serious news to the reader. The arrival of the yellow media which brought along a more sensational approach to news became also visible in more serious papers. This phenomenon is called "tabloidisation". The yellow press can be distinguished from serious papers on the basis of its nature and presentation. These characteristics can be divided into three categories, namely news themes, language and style, and presentation. The yellow press can be recognised by the amount of news items that focus on scandals and gossip involving celebrities, sex and crime. These media communicate in a informal and an easy understandable way with the readers,. These publications often make use of crude language and the stories are mostly people-driven. The yellow press publication can be seen as an entertainment package, because the aim is to entertain the reader. A vivid news package is presented to the reader by using bold headlines, large photos, graphics, colour and a captivating front page. This study investigates the characteristics of the yellow press. It investigates whether and how these characteristics are presented under the categories news themes, language, style and presentation of three local daily tabloids, namely the Kaapse Son, Daily Voice and the Daily Sun.
Thesis (M.A. (Communication Studies))--North-West University, Potchefstroom Campus, 2009.

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Di, Paolo Osvaldo. „CADÁVERES EN EL ARMARIO: EL POLICIAL PALIMPSÉSTICO EN LA LITERATURA ARGENTINA CONTEMPORÁNEA“. UKnowledge, 2011. http://uknowledge.uky.edu/gradschool_diss/36.

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This dissertation investigates the emergence of detective fiction and film from 1994 to the present. The corpus appears during the government of Carlos Menem and its intent to insert Argentina into a globalized economy. Poverty, insecurity and violence prevail in the Argentine society and ten detective novels, based on real-life murders, appear in 1994. Consequently, I explore each murder case, beginning with the newspaper article, and trace its transformation into short fiction, novel and/or film. The articles about the homicides follow the tendencies of the sensationalist yellow press. The writers and film directors, however, transform those stories, following and also subverting the characteristics of the classic detective fiction or the hard-boiled. In doing so, these recreations of the murder cases aim to denounce or criticize specific aspects of Argentine society: domestic violence, discrimination, stigmatization and corruption, among others.

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Dernerová, Žaneta. „Blesk - historie a vývoj nejčtenějšího deníku v České republice“. Master's thesis, 2017. http://www.nusl.cz/ntk/nusl-357694.

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This master's thesis aims at the development of the most successful Czech tabloid journal Blesk. It starts with the comparison of Czech and foreign tabloids. Subsequently, it captures the history of Czechoslovakian and Czech tabloids from the First Republic until the present day. This thesis also deals with the journals that unsuccessfully tried to compete with the journal Blesk. Nowadays, the only remaining tabloid journal is Aha!, nevertheless it is owned by the same publishing company as the journal Blesk. Further, the thesis pays attention to the main personalities who helped to shape the form of the journal. It inquires into the development of the graphical shape of the front pages and it tries to interpret their symbolical message, both by the means of qualitative analyses. Alongside that, it deals with the editors and their influence on the development of the journal, its visual form, and the choice of topics on the front pages. The journal Blesk also includes advertisements, and this thesis focuses on that too.

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Sipes, Sandra C. „I need a hero: a study of the power of the myth and yellow journalism newspaper coverage of the events prior to the Spanish-American war“. Thesis, 2006. http://hdl.handle.net/10057/564.

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Like most wars, the Spanish-American War had its heroes: the heroes who rescued Cuban prisoner Evangelina Cisneros, the heroes who gave aid to starving, suffering Cubans, and the heroes who investigated the possibility of a sinister element in the mysterious explosion of the battleship Maine. Even the yellow press could be construed as a hero since its leaders spared no expense in sending reporters to Cuba to capture the events leading up to the Spanish-American War for the American public. Designed to explore the hero and the heroic in journalistic coverage of war, this thesis involved qualitative textual analysis of front-page newspaper stories published in New York City during the Spanish-American War. Using Joseph Campbell's power of the myth and the hero as a framework, this thesis explores three major themes: 1) the story of Evangelina Cisneros, 2) the desperate situation of the Cuban people, and 3) the sinking of the battleship Maine. The following research questions are explored: What events in the nine-month period leading up to the war call for heroic action? Who were the heroes according to the yellow newspapers of Hearst and Pulitzer? How did these yellow newspaper stories mirror Campbell's concept of the mythic hero and his/her heroic journey? The analysis shows that these articles answered the human need for excitement, for drama, for a hero, and the need to be a hero.
Thesis (M.A.)--Wichita State University, College of Liberal Arts and Sciences, Elliott School of Communication.
"July 2006."
Includes bibliographic references (leaves 60-64)

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Machek, Jakub. „Pražský ilustrovaný kurýr. Masový tisk jako obraz světa obyčejných lidí“. Doctoral thesis, 2012. http://www.nusl.cz/ntk/nusl-307970.

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- Jakub Machek Prague Illustrated Courier. The Prague Penny-press as a Window into the World of Common Men. This work is dedicated to early sensational pictorial press (Pražský Illustrovaný Kurýr and its less successful predecessors) and its urban readers, as an example of the rising Fin de siècle popular culture in the Czech lands and its significance for revealing fundamental social and political changes that took place at the turn of the 20th century. During this era, even the members of lower social classes broadened their economical and cultural potency and thus capacity to absorb the development of new common culture generated by the rapid industrialization and urbanization. Production and reception of emerging popular press is analysed as a marker of the new modern urban experience and its social influences. Global pattern of successful mass daily press was, however, modified for Prague audience, its specific local beliefs, values and desires, distinctively different from those of western urban centre dwellers.

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Bücher zum Thema "Yellow press"

Daniel, Lynch. Yellow: A novel. New York: Walker, 1992.

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Walsdorf, John J. The Yellow Barn Press: A history and bibliography. Council Bluffs, Iowa: Yellow Barn Press, 2001.

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The yellow journalism: The press and America's emergence as a world power. Evanston, IL: Northwestern University Press, 2007.

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Minnesota rag: Corruption, yellow journalism, and the case that saved freedom of the press. Minneapolis: University of Minnesota Press, 2003.

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Milton, Joyce. The yellow kids: Foreign correspondents in the heyday of yellow journalism. New York: Harper & Row, 1989.

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Yellow dogs and dark horses: Thirty years on the campaign beat with John Robert Starr. Little Rock: August House, 1987.

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Ini, María Gabriela. Bartolo se pinta solo: Madres, hijas y esposas : tragedia y sátira de prensa durante la epidemia de fiebre amarilla de 1871. Buenos Aires: Ediciones Biblioteca Nacional, 2012.

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Simon, Berege, Yusuph Rachel und Media Council of Tanzania, Hrsg. Enhancing visibility and portrayal of women in Tanzanian media: A contrasting case staudy of Mwananchi Communications Limited against the Yellow Press. Dar es Salaam: Media Council of Tanzania, 2014.

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Baybakova, Larisa. In search of a modern concept of US foreign policy of the late XIX-early XX century. ru: INFRA-M Academic Publishing LLC., 2020. http://dx.doi.org/10.12737/1071748.

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The monograph of the Russian American historian is devoted to a number of conceptual problems of US foreign policy in the period of early globalization (late XIX-early XX century). The significance of the socio-economic factor is reinterpreted from the standpoint of modern theory and methodology; the role of the ideology used by the political elite to justify American expansion is traced. New interpretations of the causes and consequences of the Spanish-American war of 1898 are given: for the first time, the place of the "yellow" press in inciting anti-Spanish sentiment among ordinary Americans is shown in detail as one of the first manifestations of successful manipulation of public opinion; the level of combat capability of the American army, which achieved victory over a weaker enemy, but was unprepared to conduct an armed struggle for achieving geopolitical interests with leading European powers, is critically assessed. The archival material, first introduced into scientific circulation, traces the mediation activities of President Roosevelt As the first successful experience in the peaceful settlement of regional conflicts, and also shows the search by top officials for a new world order under the auspices of the United States, with an emphasis on the use of the principles of international arbitration. It is addressed to researchers, teachers, and students interested in the history of the United States.

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Oppenheim, E. Phillips. The Yellow Crayon (Dodo Press). Dodo Press, 2007.

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Buchteile zum Thema "Yellow press"

Borchard, Gregory A. „Yellow Journalism“. In A Narrative History of the American Press, 131–50. New York : Routledge, 2018.: Routledge, 2018. http://dx.doi.org/10.4324/9781315658667-9.

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Turner, Mark W. „Urban Encounters and Visual Play in the Yellow Book“. In Encounters in the Victorian Press, 138–60. London: Palgrave Macmillan UK, 2005. http://dx.doi.org/10.1057/9780230522565_9.

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Bradbury, Steven G. „11. National Security And The “New Yellow Press”“. In Journalism After Snowden, herausgegeben von Emily Bell und Taylor Owen, 172–85. New York Chichester, West Sussex: Columbia University Press, 2017. http://dx.doi.org/10.7312/bell17612-014.

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Sykora, Katharina. „Dorian Gray in the Mirror of the Yellow Press (Dorian Gray im Spiegel der Boulevardpresse, 1984)“. In Lexicon of Global Melodrama, 175–78. Bielefeld, Germany: transcript Verlag, 2022. http://dx.doi.org/10.14361/9783839459737-041.

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„THE YELLOW SHIRT MOVEMENT“. In The Foreign Press' Changing Perceptions of Thailand’s Monarchy, 18–19. ISEAS Publishing, 2015. http://dx.doi.org/10.1355/9789814695541-007.

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Evangelista, Stefano. „Controversies in the Periodical Press“. In Literary Cosmopolitanism in the English Fin de Siècle, 164–205. Oxford University Press, 2021. http://dx.doi.org/10.1093/oso/9780198864240.003.0005.

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This chapter argues that the periodical medium played a fundamental role in the construction of literary cosmopolitanism as a discursive phenomenon. It focuses on two periodicals launched in the fin de siècle: the American Cosmopolitan and the European Cosmopolis. The commercially oriented and middle-brow Cosmopolitan promoted cosmopolitanism as a female-gendered social identity linked to class privilege, as testified by the serialization of Elizabeth Bisland’s round-the-world trip in 1889. However, it also interrogated the cosmopolitan tendencies of modern American literature embodied by the writings of Henry James. By contrast, the short-lived Cosmopolis was a high-brow periodical that aimed to revive Kant’s Enlightenment ideal and Goethe’s notion of world literature. It was committed to multilingualism and to fighting nationalism. The chapter closes with an analysis of Cosmopolis as a competitor to the iconic 1890s English literary periodicals, the Yellow Book and The Savoy.

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„Der Klatsch der Yellow-Press – immer neu und doch das Gleiche“. In Ephemeres, 157–68. transcript-Verlag, 2005. http://dx.doi.org/10.14361/9783839403464-009.

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Uhl, Matthias. „Der Klatsch der Yellow-Press – immer neu und doch das Gleiche“. In Ephemeres, 157–68. transcript Verlag, 2005. http://dx.doi.org/10.1515/9783839403464-009.

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Cohen, Nathan. „Shund and the Tabloids: Jewish Popular Reading in Inter-War Poland“. In Polin: Studies in Polish Jewry Volume 16, 188–212. Liverpool University Press, 2003. http://dx.doi.org/10.3828/liverpool/9781874774730.003.0011.

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This chapter describes Jewish popular reading in inter-war Poland, looking at shund and the Polish tabloid press. In the first third of the twentieth century, as the Polish press was developing rapidly, sensationalist newspapers began to proliferate. While this type of press had been widespread in the United States and western Europe since the middle of the nineteenth century, it first emerged in Poland only in 1910, with Ilustrowany Kurier Codzienny (Illustrated Daily Courier) in Kraków. In Warsaw, the first tabloid newspapers, Kurier Informacyjny i Telegraficzny (Information and Telegraphic Courier) and Ekspres Poranny (Morning Express), appeared in 1922. In 1926, Kurier Informacyjny i Telegraficzny changed its name, now printed in red, to Kurier Czerwony (Red Courier). In time, the colour red became emblematic of sensationalist newspapers in Poland, and they were nicknamed czerwoniaki (Reds), similar to the ‘yellow’ press in the West.

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„29. »Yellow peril«, yellow press und Kriegsfurcht: Das Krisenmanagement der Regierung Roosevelt zwischen San Francisco und Tokyo, 1906–1908“. In Vermiedene Kriege, herausgegeben von Militärgeschichtliches Forschungsam, 579–602. Berlin, Boston: De Gruyter, 1997. http://dx.doi.org/10.1515/9783486830347-031.

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Berichte der Organisationen zum Thema "Yellow press"

Gafni, Yedidya, und Vitaly Citovsky. Molecular interactions of TYLCV capsid protein during assembly of viral particles. United States Department of Agriculture, April 2007. http://dx.doi.org/10.32747/2007.7587233.bard.

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Tomato yellow leaf curl geminivirus (TYLCV) is a major pathogen of cultivated tomato, causing up to 100% crop loss in many parts of the world. The present proposal, a continuation of a BARD-funded project, expanded our understanding of the molecular mechanisms by which CP molecules, as well as its pre-coat partner V2, interact with each other (CP), with the viral genome, and with cellular proteins during assembly and movement of the infectious virions. Specifically, two major objectives were proposed: I. To study in detail the molecular interactions between CP molecules and between CP and ssDNA leading to assembly of infectious TYLCV virions. II. To study the roles of host cell factors in TYLCV assembly. Our research toward these goals has produced the following major achievements: • Characterization of the CP nuclear shuttling interactor, karyopherin alpha 1, its pattern of expression and the putative involvement of auxin in regulation of its expression. (#1 in our list of publication, Mizrachy, Dabush et al. 2004). • Identify a single amino acid in the capsid protein’s sequence that is critical for normal virus life-cycle. (#2 in our list of publications, Yaakov, Levy et al. in preparation). • Development of monoclonal antibodies with high specificity to the capsid protein of TYLCV. (#3 in our list of publications, Solmensky, Zrachya et al. in press). • Generation of Tomato plants resistant to TYLCV by expressing transgene coding for siRNA targeted at the TYLCV CP. (#4 in our list of publications, Zrachya, Kumar et al. in press). •These research findings provided significant insights into (i) the molecular interactions of TYLCV capsid protein with the host cell nuclear shuttling receptor, and (ii) the mechanism by which TYLCV V2 is involved in the silencing of PTGS and contributes to the virus pathogenicity effect. Furthermore, the obtained knowledge helped us to develop specific strategies to attenuate TYLCV infection, for example, by blocking viral entry into and/or exit out of the host cell nucleus via siRNA as we showed in our publication recently (# 4 in our list of publications). Finally, in addition to the study of TYLCV nuclear import and export, our research contributed to our understanding of general mechanisms for nucleocytoplasmic shuttling of proteins and nucleic acids in plant cells. Also integration for stable transformation of ssDNA mediated by our model pathogen Agrobacterium tumefaciens led to identification of plant specific proteins involved.

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Grumet, Rebecca, und Benjamin Raccah. Identification of Potyviral Domains Controlling Systemic Infection, Host Range and Aphid Transmission. United States Department of Agriculture, Juli 2000. http://dx.doi.org/10.32747/2000.7695842.bard.

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Potyviruses form one of the largest and most economically important groups of plant viruses. Individual potyviruses and their isolates vary in symptom expression, host range, and ability to overcome host resistance genes. Understanding factors influencing these biological characteristics is of agricultural importance for epidemiology and deployment of resistance strategies. Cucurbit crops are subject to severe losses by several potyviruses including the highly aggressive and variable zucchini yellow mosaic virus (ZYMV). In this project we sought to investigate protein domains in ZYMV that influence systemic infection and host range. Particular emphasis was on coat protein (CP), because of known functions in both cell to cell and long distance movement, and helper component-protease (HC-Pro), which has been implicated to play a role in symptom development and long distance movement. These two genes are also essential for aphid mediated transmission, and domains that influence disease development may also influence transmissibility. The objectives of the approved BARD project were to test roles of specific domains in the CP and HC-Pro by making sequence alterations or switches between different isolates and viruses, and testing for infectivity, host range, and aphid transmissibility. These objectives were largely achieved as described below. Finally, we also initiated new research to identify host factors interacting with potyviral proteins and demonstrated interaction between the ZYMV RNA dependent RNA polymerase and host poly-(A)-binding protein (Wang et al., in press). The focus of the CP studies (MSU) was to investigate the role of the highly variable amino terminus (NT) in host range determination and systemic infection. Hybrid ZYMV infectious clones were produced by substituting the CP-NT of ZYMV with either the CP-NT from watermelon mosaic virus (overlapping, but broader host range) or tobacco etch virus (TEV) (non- overlapping host range) (Grumet et al., 2000; Ullah ct al., in prep). Although both hybrid viruses initially established systemic infection, indicating that even the non-cucurbit adapted TEV CP-NT could facilitate long distance transport in cucurbits, after approximately 4-6, the plants inoculated with the TEV-CPNT hybrid exhibited a distinct recovery of reduced symptoms, virus titer, and virus specific protection against secondary infection. These results suggest that the plant recognizes the presence of the TEV CP-NT, which has not been adapted to infection of cucurbits, and initiates defense responses. The CP-NT also appears to play a role in naturally occurring resistance conferred by the zym locus in the cucumber line 'Dina-1'. Patterns of virus accumulation indicated that expression of resistance is developmentally controlled and is due to a block in virus movement. Switches between the core and NT domains of ZYMV-NAA (does not cause veinal chlorosis on 'Dina-1'), and ZYMV-Ct (causes veinal chlorosis), indicated that the resistance response likely involves interaction with the CP-NT (Ullah and Grumet, submitted). At the Volcani Center the main thrust was to identify domains in the HC-Pro that affect symptom expression or aphid transmissibility. From the data reported in the first and second year report and in the attached publications (Peng et al. 1998; Kadouri et al. 1998; Raccah et al. 2000: it was shown that: 1. The mutation from PTK to PAK resulted in milder symptoms of the virus on squash, 2. Two mutations, PAK and ATK, resulted in total loss of helper activity, 3. It was established for the first time that the PTK domain is involved in binding of the HC-Pro to the potyvirus particle, and 4. Some of these experiments required greater amount of HC-Pro, therefore a simpler and more efficient purification method was developed based on Ni2+ resin.

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