Bibliografías temáticas / Actin cytoskeleton

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Literatura académica sobre el tema "Actin cytoskeleton"

Autor: Grafiati
Publicado: 4 de junio de 2021
Última modificación: 26 de julio de 2025

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Artículos de revistas sobre el tema "Actin cytoskeleton"

1

Liu, Yi, Keyvan Mollaeian, and Juan Ren. "An Image Recognition-Based Approach to Actin Cytoskeleton Quantification." Electronics 7, no. 12 (2018): 443. http://dx.doi.org/10.3390/electronics7120443.

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Quantification of the actin cytoskeleton is of prime importance to unveil the cellular force sensing and transduction mechanism. Although fluorescence imaging provides a convenient tool for observing the morphology of the actin cytoskeleton, due to the lack of approaches to accurate actin cytoskeleton quantification, the dynamics of mechanotransduction is still poorly understood. Currently, the existing image-based actin cytoskeleton analysis tools are either incapable of quantifying both the orientation and the quantity of the actin cytoskeleton simultaneously or the quantified results are su
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Breuer, David, Alexander Ivakov, Arun Sampathkumar, Florian Hollandt, Staffan Persson, and Zoran Nikoloski. "Quantitative analyses of the plant cytoskeleton reveal underlying organizational principles." Journal of The Royal Society Interface 11, no. 97 (2014): 20140362. http://dx.doi.org/10.1098/rsif.2014.0362.

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The actin and microtubule (MT) cytoskeletons are vital structures for cell growth and development across all species. While individual molecular mechanisms underpinning actin and MT dynamics have been intensively studied, principles that govern the cytoskeleton organization remain largely unexplored. Here, we captured biologically relevant characteristics of the plant cytoskeleton through a network-driven imaging-based approach allowing us to quantitatively assess dynamic features of the cytoskeleton. By introducing suitable null models, we demonstrate that the plant cytoskeletal networks exhi
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Jack, R. M., R. M. Ezzell, J. Hartwig, and D. T. Fearon. "Differential interaction of the C3b/C4b receptor and MHC class I with the cytoskeleton of human neutrophils." Journal of Immunology 137, no. 12 (1986): 3996–4003. http://dx.doi.org/10.4049/jimmunol.137.12.3996.

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Abstract As measured by fluorescence microscopy and radioligand binding, C3b/C4b receptors (CR1) became attached to the detergent-insoluble cytoskeleton of human neutrophils when receptors were cross-linked by affinity-purified polyclonal F(ab')2 anti-CR1, dimeric C3b, or Fab monoclonal anti-CR1 followed by F(ab')2 goat anti-mouse F(ab')2. CR1 on neutrophils bearing monovalent anti-CR1 was not attached to the cytoskeleton. In contrast, cross-linked CR1 on erythrocytes and cross-linked MHC Class I on neutrophils were not cytoskeleton associated. A possible role for filamentous actin (F-actin) i
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Vaduva, Gabriela, Nancy C. Martin, and Anita K. Hopper. "Actin-binding Verprolin Is a Polarity Development Protein Required for the Morphogenesis and Function of the Yeast Actin Cytoskeleton." Journal of Cell Biology 139, no. 7 (1997): 1821–33. http://dx.doi.org/10.1083/jcb.139.7.1821.

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Yeast verprolin, encoded by VRP1, is implicated in cell growth, cytoskeletal organization, endocytosis and mitochondrial protein distribution and function. We show that verprolin is also required for bipolar bud-site selection. Previously we reported that additional actin suppresses the temperature-dependent growth defect caused by a mutation in VRP1. Here we show that additional actin suppresses all known defects caused by vrp1-1 and conclude that the defects relate to an abnormal cytoskeleton. Using the two-hybrid system, we show that verprolin binds actin. An actin-binding domain maps to th
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Bezanilla, Magdalena, Amy S. Gladfelter, David R. Kovar, and Wei-Lih Lee. "Cytoskeletal dynamics: A view from the membrane." Journal of Cell Biology 209, no. 3 (2015): 329–37. http://dx.doi.org/10.1083/jcb.201502062.

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Many aspects of cytoskeletal assembly and dynamics can be recapitulated in vitro; yet, how the cytoskeleton integrates signals in vivo across cellular membranes is far less understood. Recent work has demonstrated that the membrane alone, or through membrane-associated proteins, can effect dynamic changes to the cytoskeleton, thereby impacting cell physiology. Having identified mechanistic links between membranes and the actin, microtubule, and septin cytoskeletons, these studies highlight the membrane’s central role in coordinating these cytoskeletal systems to carry out essential processes,
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SAUMET, Anne, Nando de JESUS, Chantal LEGRAND та Véronique DUBERNARD. "Association of thrombospondin-1 with the actin cytoskeleton of human thrombin-activated platelets through an αIIbβ3- or CD36-independent mechanism". Biochemical Journal 363, № 3 (2002): 473–82. http://dx.doi.org/10.1042/bj3630473.

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Thrombospondin-1 (TSP-1) is an adhesive glycoprotein which, when secreted from α-granules of activated platelets, can bind to the cell surface and participate in platelet aggregate formation. In this study, we show that thrombin activation leads to the rapid and specific association of a large amount of secreted α-granular TSP-1 with the actin cytoskeleton. This cytoskeletal association of TSP-1 was correlated with platelet secretion, but not aggregation, and was inhibited by cytochalasin D, an inhibitor of actin polymerization. Association of TSP-1 with the actin cytoskeleton was mediated by
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Ballestrem, C., B. Wehrle-Haller, and B. A. Imhof. "Actin dynamics in living mammalian cells." Journal of Cell Science 111, no. 12 (1998): 1649–58. http://dx.doi.org/10.1242/jcs.111.12.1649.

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The actin cytoskeleton maintains the cellular architecture and mediates cell movements. To explore actin cytoskeletal dynamics, the enhanced green fluorescent protein (EGFP) was fused to human β-actin. The fusion protein was incorporated into actin fibers which became depolymerized upon cytochalasin B treatment. This functional EGFP-actin construct enabled observation of the actin cytoskeleton in living cells by time lapse fluorescence microscopy. Stable expression of the construct was obtained in mammalian cell lines of different tissue origins. In stationary cells, actin rich, ring-like stru
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Holly, Stephen P., and Kendall J. Blumer. "Pak-Family Kinases Regulate Cell and Actin Polarization Throughout the Cell Cycle of Saccharomyces cerevisiae." Journal of Cell Biology 147, no. 4 (1999): 845–56. http://dx.doi.org/10.1083/jcb.147.4.845.

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During the cell cycle of the yeast Saccharomyces cerevisiae, the actin cytoskeleton and cell surface growth are polarized, mediating bud emergence, bud growth, and cytokinesis. We have determined whether p21-activated kinase (PAK)-family kinases regulate cell and actin polarization at one or several points during the yeast cell cycle. Inactivation of the PAK homologues Ste20 and Cla4 at various points in the cell cycle resulted in loss of cell and actin cytoskeletal polarity, but not in depolymerization of F-actin. Loss of PAK function in G1 depolarized the cortical actin cytoskeleton and bloc
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Uray, Karen, Evelin Major, and Beata Lontay. "MicroRNA Regulatory Pathways in the Control of the Actin–Myosin Cytoskeleton." Cells 9, no. 7 (2020): 1649. http://dx.doi.org/10.3390/cells9071649.

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MicroRNAs (miRNAs) are key modulators of post-transcriptional gene regulation in a plethora of processes, including actin–myosin cytoskeleton dynamics. Recent evidence points to the widespread effects of miRNAs on actin–myosin cytoskeleton dynamics, either directly on the expression of actin and myosin genes or indirectly on the diverse signaling cascades modulating cytoskeletal arrangement. Furthermore, studies from various human models indicate that miRNAs contribute to the development of various human disorders. The potentially huge impact of miRNA-based mechanisms on cytoskeletal elements
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Vindin, Howard, Leanne Bischof, Peter Gunning, and Justine Stehn. "Validation of an Algorithm to Quantify Changes in Actin Cytoskeletal Organization." Journal of Biomolecular Screening 19, no. 3 (2013): 354–68. http://dx.doi.org/10.1177/1087057113503494.

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The actin cytoskeleton plays an important role in most, if not all, processes necessary for cell survival. Given the fundamental role that the actin cytoskeleton plays in the progression of cancer, it is an ideal target for chemotherapy. Although it is possible to image the actin cytoskeleton in a high-throughput manner, there is currently no validated method to quantify changes in the cytoskeleton in the same capacity, which makes research into its organization and the development of anticytoskeletal drugs difficult. We have validated the use of a linear feature detection algorithm, allowing
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Tesis sobre el tema "Actin cytoskeleton"

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Brown, Jennifer. "Investigating the actin cytoskeleton in cancer." Thesis, University of Glasgow, 2016. http://theses.gla.ac.uk/7266/.

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Dynamic alterations in the actin cytoskeleton, under the regulation of the Rho/ROCK pathway, permit cell motility, cell-to-cell and cell-to-matrix adhesion, and have also been shown to participate in apoptosis and cell proliferation. These facets of cellular behaviour all have the capacity to become dysregulated in cancer; components of the Rho/ROCK pathway are known to play varying roles in these processes, both within primary tumours and within the tumour microenvironment. The LIM kinases are phosphorylated and activated by ROCK, leading to inactivation of cofilin and subsequent stabilisatio
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2

Huber, Florian. "Emergent structure formation of the actin cytoskeleton." Doctoral thesis, Universitätsbibliothek Leipzig, 2012. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-86666.

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Anders als menschengemachte Maschinen verfügen Zellen über keinen festgeschriebenen Bauplan und die Positionen einzelner Elemente sind häufig nicht genau festgelegt, da die Moleküle diffusiven Zufallsbewegungen unterworfen sind. Darüber hinaus sind einzelne Bauteile auch nicht auf eine einzelne Funktion festgelegt, sondern können parallel in verschiedene Prozesse einbezogen sein. Basierend auf Selbstorganisation und Selbstassemblierung muß die Organisation von Anordnung und Funktion einer lebenden Zelle also bereits in ihren einzelnen Komponenten inhärent enthalten sein. Die intrazelluläre Or
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Förster, Florian. "Targeting the actin cytoskeleton with natural compounds." Diss., Ludwig-Maximilians-Universität München, 2014. http://nbn-resolving.de/urn:nbn:de:bvb:19-168914.

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Targeting the cytoskeleton (CSK) of cancer cells offers a valuable strategy in cancer therapy. Whereas drugs which address microtubule CSK such as vinca alkaloids or taxanes are well established in the clinic, compounds binding to the actin CSK are still far away from their therapeutical application. One reason might be the lacking knowledge on their mode of cytotoxicity and moreover their tumor specific mechanism of action. We used the myxobacterial compound Chondramide as a tool to first elucidate the mechanisms of cytotoxicity by actin targeting in different breast cancer cells, namely MC
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Kim, Taeyoon Ph D. Massachusetts Institute of Technology. "Simulation of actin cytoskeleton structure and rheology." Thesis, Massachusetts Institute of Technology, 2007. http://hdl.handle.net/1721.1/39875.

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Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2007.<br>Includes bibliographical references (p. 81-87).<br>Structures consisting of G-actin or other filament-forming monomers show a variety of morphologies with widely different properties in regard to pore size, degree of isotropy, and extent of cross-linking. These characteristics are primarily determined by the concentration and feature of proteins which cross-link filaments, but little is known how the filament-forming monomers and cross-linking proteins are organized in order to produce various netwo
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Gallinger, Julia. "WH2 domains and actin variants as multifunctional organizers of the actin cytoskeleton." Diss., Ludwig-Maximilians-Universität München, 2013. http://nbn-resolving.de/urn:nbn:de:bvb:19-161698.

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Actin is one of the most abundant proteins in eukaryotic cells and regulation of the microfilament system is crucial for a wide range of cellular functions including cell shape, cell motility, cell division and membrane dynamics. The aim of this thesis was (1) to gain a better understanding of the function of distinct actin binding domains in the regulation of the actin cytoskeleton and (2) to elucidate the role of actin variants. WH2 domains (WH2, Wiskott-Aldrich syndrome protein homology 2) are ubiquitous multifunctional regulators of actin dynamics. The protein Spire contains four central
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Speldewinde, Shaun. "Prions, autophagy, ageing and actin cytoskeleton in yeast." Thesis, University of Manchester, 2017. https://www.research.manchester.ac.uk/portal/en/theses/prions-autophagy-ageing-and-actin-cytoskeleton-in-yeast(03085d7f-283a-40e1-bcf7-d9533ff2e2fc).html.

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Prions are infectious protein entities capable of self-replication. Prions are the causal agents behind the transmissible spongiform encephalopathies causing neurodegeneration and death in affected organisms. Prions have been identified in yeast with the best-characterized prions being [PSI+] and [PIN+], whose respective native proteins are the Sup35 translation termination factor and Rnq1 (function unknown). Autophagy is a cellular housekeeping mechanism mediating the degradation of damaged proteins and superfluous organelles. It is a highly sequential process regulated by autophagy related g
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Hayot, Caroline. "Mise au point d'une stratégie pharmacologique originale pour l'obtention de composés anti-cancéreux anti-migratoires." Doctoral thesis, Universite Libre de Bruxelles, 2006. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/210860.

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La migration cellulaire est une étape clé intervenant à un stade précoce de la dissémination des cellules cancéreuses dans l’organisme, et est donc responsable de la formation des métastases qui tuent environ nonante pourcent des patients atteints de cancer. De plus, ces cellules migrantes résistent à l’apoptose grâce à l’activation constitutive de voies de signalisation anti-apoptotiques, et développent donc une résistance vis-à-vis des traitements anti-cancéreux actuels qui sont généralement pro-apoptotiques. Nous avons pris pour cible ce processus de migration cellulaire dans l’espoir d’ide
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Thodey, Catherine. "Actin cytoskeleton dynamics mediate sugar response in Arabidopsis thaliana." Thesis, University of East Anglia, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.518364.

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Price, Leo Sebastian. "Secretion and the actin cytoskeleton in rat mast cells." Thesis, University College London (University of London), 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307776.

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Filippi, Beatrice Maria. "Cellular effects of phosphoinositide derivatives on the actin cytoskeleton." Thesis, Open University, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.424620.

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Libros sobre el tema "Actin cytoskeleton"

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Jockusch, Brigitte M., ed. The Actin Cytoskeleton. Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-46371-1.

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Mannherz, Hans Georg, ed. The Actin Cytoskeleton and Bacterial Infection. Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-50047-8.

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E, Estes James, Higgins Paul J, and International Conference on the Biophysics, Biochemistry, and Cell Biology of Actin (1992 : Troy, N.Y.), eds. Actin: Biophysics, biochemistry, and cell biology. Plenum Press, 1994.

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G, Dos Remedios Cristobal, and Chhabra Deepak, eds. Actin-binding proteins and disease. Springer, 2008.

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G, Dos Remedios Cristobal, and Chhabra Deepak, eds. Actin-binding proteins and disease. Springer, 2008.

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J, Staiger C., ed. Actin: A dynamic framework for multiple plant cell functions. Kluwer Academic Publishers, 2000.

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Sing, Cierra Nicole. Aging Actin' Up: A novel aging determinant regulates the actin cytoskeleton, nutrient sensing, and lifespan in Saccharomyces cerevisiae. [publisher not identified], 2021.

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1933-, Sugi Haruo, and Pollack Gerald H, eds. Mechanism of myofilament sliding in muscle contraction. Plenum Press, 1993.

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Malapitan, Irish Ann. Mapping an F-actin and cytoskeletal binding region in the basic domain of the mouse LSP1 protein. National Library of Canada, 1994.

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Rahlenbeck, Christine. Influence of horizontal rotation on the starch metabolism and the cytoskeletal component f-actin in vicia faba l. leaves: Effect of omnilateral gravistimulation and microgravity simulation. [s.n.], 1993.

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Capítulos de libros sobre el tema "Actin cytoskeleton"

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Isenberg, Gerhard. "Actin and Actin-Associated Proteins." In Cytoskeleton Proteins. Springer Berlin Heidelberg, 1995. http://dx.doi.org/10.1007/978-3-642-79632-6_8.

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Wang, Juan, Ruihui Zhang, Ming Chang, et al. "Actin Cytoskeleton." In Cell Biology. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4614-7881-2_6-1.

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Bershadsky, Alexander D., and Juri M. Vasiliev. "Systems of Actin Filaments." In Cytoskeleton. Springer US, 1988. http://dx.doi.org/10.1007/978-1-4684-5278-5_2.

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Baluška, F., and S. Mancuso. "Actin Cytoskeleton and Action Potentials: Forgotten Connections." In The Cytoskeleton. Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-33528-1_5.

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Dugina, Vera, Richard Arnoldi, Paul A. Janmey, and Christine Chaponnier. "ACTIN." In Cytoskeleton and Human Disease. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-788-0_1.

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Noegel, A. A., B. Köppel, U. Gottwald, W. Witke, R. Albrecht, and M. Schleicher. "Actin and Actin-Binding Proteins in the Motility of Dictyostelium." In The Cytoskeleton. Springer Berlin Heidelberg, 1995. http://dx.doi.org/10.1007/978-3-642-79482-7_13.

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Kelber, Jonathan A., and Richard L. Klemke. "The Actin Cytoskeleton." In Cellular Domains. John Wiley & Sons, Inc., 2011. http://dx.doi.org/10.1002/9781118015759.ch12.

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Amos, Linda A., and W. Bradshaw Amos. "Actin Filaments." In Molecules of the Cytoskeleton. Macmillan Education UK, 1991. http://dx.doi.org/10.1007/978-1-349-21739-7_3.

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Aktories, Klaus, Carsten Schwan, and Alexander E. Lang. "ADP-Ribosylation and Cross-Linking of Actin by Bacterial Protein Toxins." In The Actin Cytoskeleton. Springer International Publishing, 2016. http://dx.doi.org/10.1007/164_2016_26.

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Viita, Tiina, and Maria K. Vartiainen. "From Cytoskeleton to Gene Expression: Actin in the Nucleus." In The Actin Cytoskeleton. Springer International Publishing, 2016. http://dx.doi.org/10.1007/164_2016_27.

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Actas de conferencias sobre el tema "Actin cytoskeleton"

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Liu, Yi, and Juan Ren. "Modeling and Control of Dynamic Cellular Mechanotransduction: Part I — Actin Cytoskeleton Quantification." In ASME 2018 Dynamic Systems and Control Conference. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/dscc2018-9180.

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Living cells respond to external stimuli through the reorganization of the actin cytoskeleton, and the actin cytoskeleton significantly affects the cellular mechanical behavior. However, due to the lack of approaches to actin cytoskeleton quantification, the dynamics of mechanotransduction is still poorly understood. In this study, we propose an image recognition-based quantification (IRQ) approach to actin cytoskeleton quantification. IRQ quantifies the actin cytoskeleton through three parameters: the partial actin-cytoskeletal deviation (PAD), the total actin-cytoskeletal deviation (TAD) and
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Wen, Shin-Min, and Pen-hsiu Grace Chao. "Spatial Actin Structure Does Not Correlate With Nuclear Organization." In ASME 2013 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/sbc2013-14167.

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Cells in situ exhibit a great variety of morphologies that intimately relates to phenotypic controls. Cell morphology regulates cytoskeletal organization, which in turn influences nuclear shape and organization [1–4]. The actomyosin cytoskeleton is connected to a structure known as the linker of nucleoskeleton and cytoskeleton (LINC) complex located on the nuclear membrane. LINC is believed to transmit deformation of the actin cytoskeleton into the nucleus and nucleoskeleton, change nuclear shape as well as chromatin conformation, and modulate gene expression [5, 6]. Khatau and coworkers repor
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Dutta, Surjendu Bikash, Anders Kokkvoll Engdahl, Stefan Belle, et al. "Waveguide chip based super-resolution microscopy for T cell imaging." In Integrated Photonics Research, Silicon and Nanophotonics. Optica Publishing Group, 2022. http://dx.doi.org/10.1364/iprsn.2022.itu1b.6.

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Imaging and the quantitative estimation of T cell actin cytoskeletal dynamics are important to describe immunological processes. This study presents waveguide chip based super-resolution imaging of the filamentous actin cytoskeleton of Jurkat T cells.
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Kiran, Kranthi, Sanjay Govindjee, and Mohammad R. K. Mofrad. "On the Cytoskeleton and Soft Glassy Rheology." In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-176736.

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Cytoskeleton is an integrated system of biomolecules, providing the cell with shape, integrity, and internal spatial organization. Cytoskeleton is a three-dimensional (3-D) network consisting of a complex mixture of actin filaments, intermediate filaments and microtubules that are collectively responsible for the main structural properties and motilities of the cell. A wide range of theoretical models have been proposed for cytoskeletal mechanics, ranging from continuum models for cell deformation to actin filament-based models for cell motility [1]. Numerous experimental techniques have also
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Allen, Kathleen B., and Bradley Layton. "A Mechanical Model for Cytoskeleton and Membrane Interactions in Neuronal Growth Cones." In ASME 2007 International Mechanical Engineering Congress and Exposition. ASMEDC, 2007. http://dx.doi.org/10.1115/imece2007-42008.

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Revealing the molecular events of neuronal growth is critical to obtaining a deeper understanding of nervous system development, neural injury response, and neural tissue engineering. Central to this is the need to understand the mechanical interactions among the cytoskeleton and the cell membrane, and how these interactions affect the overall growth mechanics of neurons. Using ANSYS, the force produced by a cytoskeletal protein acting against a deformable membrane was modeled, and the deformation, stress, and strain were computed for the membrane. Parameters to represent the flexural rigiditi
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Dangaria, Jhanvi H., and Peter J. Butler. "Interaction of Shear Stress, Myosin II, and Actin in Dynamic Modulation of Endothelial Cell Microrheology." In ASME 2008 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2008. http://dx.doi.org/10.1115/sbc2008-192947.

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The endothelial cell (EC) cytoskeleton mediates several biological functions such as adhesion, migration, phagocytosis, cell division, and mechanosensitivity. These functions are carried out in part through dynamic cytoskeletal polymerization, modulation of crosslinking, and development of tension between intracellular organelles and the extracellular matrix via focal adhesion plaques. One important component of the cytoskeleton is actin which polymerizes into filaments and is thought to be prestressed by virtue of crosslinking proteins such as α-actinin, filamin and myosin II molecular motors
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Horiguchi, Atsushi, and Toshihiko Shiraishi. "Study on a Cell Mechanosensing System by Measuring Structural Deformation and Biochemical Response." In ASME 2015 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/imece2015-51456.

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Mechanical stimulation induces new bone formation in vivo and promotes the metabolic activity and the gene expression of osteoblasts in vitro. It was reported that biochemical signals of osteoblasts to sense mechanical stimulation are activated according to their actin cytoskeletal deformation. However, there have been not so many researches on the relationship between cytoskeletal deformation and biochemical response. Here we show an original method to investigate a cell mechanosensing system and the quantitative relationship between the deformation of cytoskeletal structure and the change of
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Shibatay, N., K. Tanaka, K. Okamoto, and T. Onji. "REORGANIZATION OF ACTIN AND MYOSIN IN THE ACTIVATED PLATELETS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643539.

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This study was done to clarify the intracellular dynamic arrangements of myosin(My) and actin(Ac) in activation process of human platelets (PLs) from unactivated to activated stage (clot retraction) in electron microscopy. The observation of unactivated PLs was done either in the fresh whole blood fixed directly with 0.1 % glutaraldehyde or in PLs isolated by gel filtration of platelet rich plasma(PRP) containing prostaglandin I2 (10 ng/ml). The isolated PLs mounted on a glass cover slip were used as activated PLs (adrerent ones). The contracted PLs were prepared in PRP incubated with thrombin
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Bidone, Tamara C., Marco A. Deriu, Francesco Mastrangelo, Giacomo Di Benedetto, Monica Soncini, and Umberto Morbiducci. "Elastic Network Modeling of Actin Filaments." In ASME 2010 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2010. http://dx.doi.org/10.1115/sbc2010-19074.

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Cell mechanics depends on the mechanical properties of actin microfilaments (MFs), microtubules (MTs) and intermediate filaments (IFs), that build the cytoskeleton. Actin microfilaments are the most abundant components and play significant roles in various cellular processes [1]. Among them, the mechanical properties of MFs are essential for the functions of the cytoskeleton and are directly related to their molecular architecture.
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Oswald, Elizabeth S., Pen-hsiu Grace Chao, J. Chloe Bulinski, Gerard A. Ateshian, and Clark T. Hung. "The Role of Microtubule Organization in Chondrocyte Response to Osmotic Loading." In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-176634.

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The cytoskeleton, including actin filaments and microtubules, provides chondrocytes with structure, cytoplasmic organization, and intracellular transport. The cytoskeleton is known to be involved in cellular responses to physiologic mechanical and osmotic loading signals, including morphological changes and mechanostransduction [1, 2]. Here, we examine microtubule (MT) involvement in volume response of chondrocytes to osmotic loading, as well as organization of stable MT with hypoosmotic loading. We also explore the hypothesis that chondrocytes from different zones of cartilage possess cytoske
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Informes sobre el tema "Actin cytoskeleton"

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Sadot, Einat, Christopher Staiger, and Zvi Kam Weizmann. functional genomic screen for new plant cytoskeletal proteins and the determination of their role in actin mediated functions and guard cells regulation. United States Department of Agriculture, 2003. http://dx.doi.org/10.32747/2003.7587725.bard.

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The original objectives of the approved proposal were: 1. To construct a YFP fused Arabidopsis cDNA library in a mammalian expression vector. 2. To infect the library into a host fibroblast cell line and to screen for new cytoskeletal associated proteins using an automated microscope. 3. Isolate the new genes. 4. Characterize their role in plants. The project was approved as a feasibility study to allow proof of concept that would entail building the YFP library and picking up a couple of positive clones using the fluorescent screen. We report here on the construction of the YFP library, the d
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Ramesh, Vijaya. Neurofibromatosis 2 Tumor Suppressor Protein, Merlin, in Cellular Signaling to Actin Cytoskeleton. Defense Technical Information Center, 2000. http://dx.doi.org/10.21236/ada395581.

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Philosoph-Hadas, Sonia, Peter B. Kaufman, Shimon Meir, and Abraham H. Halevy. Inhibition of the Gravitropic Shoot Bending in Stored Cut Flowers Through Control of Their Graviperception: Involvement of the Cytoskeleton and Cytosolic Calcium. United States Department of Agriculture, 2005. http://dx.doi.org/10.32747/2005.7586533.bard.

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Original objectives: The basic goal of the present project was to study the mechanism involved in shoot graviperception and early transduction, in order to determine the sequence of events operating in this process. This will enable to control the entire process of gravity-induced differential growth without affecting vertical growth processes essential for development. Thus, several new postulated interactions, operating at the perception and early transduction stages of the signaling cascade leading to auxin-mediated bending, were proposed to be examined in snapdragon spikes and oat shoot pu
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Syed, Aleem. Spatial and temporal dynamics of receptor for advanced glycation endproducts, integrins, and actin cytoskeleton as probed with fluorescence-based imaging techniques. Office of Scientific and Technical Information (OSTI), 2016. http://dx.doi.org/10.2172/1342583.

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Stoyanova, Tihomira, Veselina Uzunova, Albena Momchilova, Rumiana Tzoneva, and Iva Ugrinova. The Treatment of Breast Cancer Cells with Erufosine Leads to Actin Cytoskeleton Reorganization, Inhibition of Cell Motility, Cell Cycle Arrest and Apoptosis. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, 2021. http://dx.doi.org/10.7546/crabs.2021.01.11.

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Philosoph-Hadas, Sonia, Peter Kaufman, Shimon Meir, and Abraham Halevy. Signal Transduction Pathway of Hormonal Action in Control and Regulation of the Gravitropic Response of Cut Flowering Stems during Storage and Transport. United States Department of Agriculture, 1999. http://dx.doi.org/10.32747/1999.7695838.bard.

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Original objectives: The basic goal of the present project was to increase our understanding of the cellular mechanisms operating during the gravitropic response of cut flowers, for solving their bending problem without affecting flower quality. Thus, several elements operating at the 3 levels o the gravity-induced signal transduction pathway, were proposed to be examined in snapdragon stems according to the following research goals: 1) Signaling: characterize the signal transduction pathway leading to the gravitropic response, regarding the involvement of [Ca2+]cyt as a mediator of IAA moveme
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Sadot, Einat, Christopher Staiger, and Mohamad Abu-Abied. Studies of Novel Cytoskeletal Regulatory Proteins that are Involved in Abiotic Stress Signaling. United States Department of Agriculture, 2011. http://dx.doi.org/10.32747/2011.7592652.bard.

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In the original proposal we planned to focus on two proteins related to the actin cytoskeleton: TCH2, a touch-induced calmodulin-like protein which was found by us to interact with the IQ domain of myosin VIII, ATM1; and ERD10, a dehydrin which was found to associate with actin filaments. As reported previously, no other dehydrins were found to interact with actin filaments. In addition so far we were unsuccessful in confirming the interaction of TCH2 with myosin VIII using other methods. In addition, no other myosin light chain candidates were found in a yeast two hybrid survey. Nevertheless
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Chew, Teng-Leong. Regulation of Actin-Myosin Cytoskeletal Changes Involved in Cancer Metastasis. Defense Technical Information Center, 2001. http://dx.doi.org/10.21236/ada396798.

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Friedman, Haya, Julia Vrebalov, and James Giovannoni. Elucidating the ripening signaling pathway in banana for improved fruit quality, shelf-life and food security. United States Department of Agriculture, 2014. http://dx.doi.org/10.32747/2014.7594401.bard.

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Background : Banana being a monocot and having distinct peel and pulp tissues is unique among the fleshy fruits and hence can provide a more comprehensive understanding of fruit ripening. Our previous research which translated ripening discoveries from tomato, led to the identification of six banana fruit-associated MADS-box genes, and we confirmed the positive role of MaMADS1/2 in banana ripening. The overall goal was to further elucidate the banana ripening signaling pathway as mediated by MADS-boxtranscriptional regulators. Specific objectives were: 1) characterize transcriptional profiles
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Hansen, Peter J., and Amir Arav. Embryo transfer as a tool for improving fertility of heat-stressed dairy cattle. United States Department of Agriculture, 2007. http://dx.doi.org/10.32747/2007.7587730.bard.

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The overall objective of the current proposal is to develop procedures to improve the pregnancy rate achieved following transfer of fresh or cryopreserved embryos produced in the laboratory into heat-stress recipients. The overall hypothesis is that pregnancy rate in heat-stressed lactating cows can be improved by use of embryo transfer and that additional gains in pregnancy rate can be achieved through development of procedures to cryopreserve embryos, select embryos most likely to establish and maintain pregnancy after transfer, and to enhance embryo competence for post-transfer survival thr
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