Bibliografías temáticas / Cell migration

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Literatura académica sobre el tema "Cell migration"

Autor: Grafiati
Publicado: 4 de junio de 2021
Última modificación: 25 de julio de 2025

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Artículos de revistas sobre el tema "Cell migration"

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Trepat, Xavier, Zaozao Chen, and Ken Jacobson. "Cell Migration." Comprehensive Physiology 2, no. 4 (2012): 2369–92. https://doi.org/10.1002/j.2040-4603.2012.tb00467.x.

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AbstractCell migration is fundamental to establishing and maintaining the proper organization of multicellular organisms. Morphogenesis can be viewed as a consequence, in part, of cell locomotion, from large‐scale migrations of epithelial sheets during gastrulation, to the movement of individual cells during development of the nervous system. In an adult organism, cell migration is essential for proper immune response, wound repair, and tissue homeostasis, while aberrant cell migration is found in various pathologies. Indeed, as our knowledge of migration increases, we can look forward to, for
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Thomas, L. A., and K. M. Yamada. "Contact stimulation of cell migration." Journal of Cell Science 103, no. 4 (1992): 1211–14. http://dx.doi.org/10.1242/jcs.103.4.1211.

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Mass migrations of dense cell populations occur periodically during embryonic development. It is known that extracellular matrices, through which the cells migrate, facilitate locomotion. However, this does not explain how cells, such as neural crest, can migrate as a dense cohort of cells in essentially continuous contact with one another. We report here that unique behavioral characteristics of the migrating cells may contribute to cohesive migration. We used time-lapse video microscopy to analyze the migration of quail neural crest cells and of two crest derivatives, human melanoma cells an
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Deniz, Özdemir. "KAN0438757: A NOVEL PFKFB3 INHIBITOR THAT INDUCES PROGRAMMED CELL DEATH AND SUPPRESSES CELL MIGRATION IN NON-SMALL CELL LUNG CARCINOMA CELLS." Biotechnologia Acta 16, no. 5 (2023): 34–44. http://dx.doi.org/10.15407/biotech16.05.034.

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Aim. PFKFB3 is glycolytic activators that is overexpressed in human lung cancer and plays a crucial role in multiple cellular functions including programmed cell death. Despite the many small molecules described as PFKFB3 inhibitors, some of them have shown disappointing results in vitro and in vivo. On the other hand KAN0438757, selective and potent, small molecule inhibitor has been developed. However, the effects of KAN0438757, in non-small cell lung carcinoma cells remain unknown. Herein, we sought to decipher the effect of KAN0438757 on proliferation, migration, DNA damage, and programmed
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Ffrench-Constant, C., and R. O. Hynes. "Patterns of fibronectin gene expression and splicing during cell migration in chicken embryos." Development 104, no. 3 (1988): 369–82. http://dx.doi.org/10.1242/dev.104.3.369.

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A variety of evidence suggests that fibronectin (FN) promotes cell migration during embryogenesis, and it has been suggested that the deposition of FN along migratory pathways may also play a role in cell guidance. In order to investigate such a role for FN, it is important to determine the relative contribution of migrating and pathway-forming cells to the FN in the migratory track, as any synthesis of FN by the migrating cells might be expected to mask guidance cues provided by the exogenous FN from pathway-forming cells. We have therefore used in situ hybridization to determine in developin
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Deryugina, E. I., and M. A. Bourdon. "Tenascin mediates human glioma cell migration and modulates cell migration on fibronectin." Journal of Cell Science 109, no. 3 (1996): 643–52. http://dx.doi.org/10.1242/jcs.109.3.643.

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The role of tenascin in mediating tumor cell migration was studied using two cell migration models. In migration/invasion Transwell assays U251.3 glioma cells rapidly migrated through the 8 mu m pore size membranes onto tenascin- and fibronectin-coated surfaces. In this assay the number of cells migrating onto tenascin was 52.2 +/- 9.6% greater than on fibronectin within 4 hours. To assess cell migration rates and cell morphology, U251.3 migration was examined in a two-dimension spheroid outgrowth assay. The radial distance migrated by U251.3 cells from tumor spheroids was found to be 53.8 +/-
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Torrence, S. A. "Positional cues governing cell migration in leech neurogenesis." Development 111, no. 4 (1991): 993–1005. http://dx.doi.org/10.1242/dev.111.4.993.

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The stereotyped distribution of identified neurons and glial cells in the leech nervous system is the product of stereotyped cell migrations and rearrangements during embryogenesis. To examine the dependence of long-distance cell migrations on positional cues provided by other tissues, embryos of Theromyzon rude were examined for the effects of selective ablation of various embryonic cell lines on the migration and final distribution of neural and glial precursor cells descended from the bilaterally paired ectodermal cell lines designated q bandlets. The results suggest that neither the commit
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Li, David, and Yu-li Wang. "Coordination of cell migration mediated by site-dependent cell–cell contact." Proceedings of the National Academy of Sciences 115, no. 42 (2018): 10678–83. http://dx.doi.org/10.1073/pnas.1807543115.

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Contact inhibition of locomotion (CIL), the repulsive response of cells upon cell–cell contact, has been the predominant paradigm for contact-mediated responses. However, it is difficult for CIL alone to account for the complex behavior of cells within a multicellular environment, where cells often migrate in cohorts such as sheets, clusters, and streams. Although cell–cell adhesion and mechanical interactions play a role, how individual cells coordinate their migration within a multicellular environment remains unclear. Using micropatterned substrates to guide cell migration and manipulate ce
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Bradley, David. "Cell migration." Materials Today 14, no. 1-2 (2011): 10. http://dx.doi.org/10.1016/s1369-7021(11)70010-4.

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Horwitz, Rick, and Donna Webb. "Cell migration." Current Biology 13, no. 19 (2003): R756—R759. http://dx.doi.org/10.1016/j.cub.2003.09.014.

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Wang, Heng, and Jiong Chen. "Cell migration: Collective cell migration is intrinsically stressful." Current Biology 34, no. 7 (2024): R275—R278. http://dx.doi.org/10.1016/j.cub.2024.02.061.

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Tesis sobre el tema "Cell migration"

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Falk, Anna. "Stem cells : proliferation, differentiation, migration /." Stockholm, 2005. http://diss.kib.ki.se/2006/91-7140-497-X/.

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Sundström, Magnus. "Signal transduction in mast cell migration /." Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2001. http://publications.uu.se/theses/91-554-5130-6/.

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SUN, Xue-Zhi, Sentaro TAKAHASHI, Chun GUI, et al. "Neuronal Migration and Neuronal Migration Disorder in Cerebral Cortex." Research Institute of Environmental Medicine, Nagoya University, 2002. http://hdl.handle.net/2237/2773.

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Chometon-Luthe, Gretel. "Epithelial cell migration on laminins." [S.l. : s.n.], 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=975579185.

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Burthem, John. "Hairy cell adhesion and migration." Thesis, University of Liverpool, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.240394.

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Dawson, M. "Mast cell migration in allergy." Thesis, University College London (University of London), 2012. http://discovery.ucl.ac.uk/1357935/.

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The symptomology associated with allergic diseases are a direct consequence of the release of pro-inflammatory mediators from mast cells following bi- or multivalent antigen cross-linking with the high affinity immunoglobulin (Ig) E receptor, FcεR1. Chemokines, small 8-15 kDa polypeptides, control the activation and recruitment of immune cells during the allergic response. Previous studies have demonstrated that co-stimulation by the chemokine, macrophage inflammatory protein-1α (Mip-1α) and cross-linking by IgE with antigen result in four phenomenon 1) enhanced degranulation in ex vivo conjun
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Erlandsson, Anna. "Neural Stem Cell Differentiation and Migration." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl.[distributör], 2003. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3546.

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Belotti, Yuri. "Microfluidic methods for investigating cell migration and cell mechanics." Thesis, University of Dundee, 2016. https://discovery.dundee.ac.uk/en/studentTheses/fb5ac03d-a752-45a1-8b95-37c8180dc7d9.

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In this thesis I explore how migratory properties of the model organism Dictyostelium discoideum are influenced by dimensionality and topology of the environment that surrounds the cell. Additionally, I sought to develop a microfluidic device able to measure mechanical properties of single cells with a sufficient throughput to account for the inherent heterogeneity of biological samples. Throughout this thesis I made use of microfabrication methods such as photo-lithography and soft-lithography, to develop ad hoc microstructured substrates. These tools enabled me to tackle different biological
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RUNYAN, CHRISTOPHER MICHAEL. "The Role of Cell Death in Germ Cell Migration." University of Cincinnati / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1210732680.

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Shuib, Anis Suhaila. "Investigation of blood cells migration in large stenosed artery." Thesis, University of Edinburgh, 2012. http://hdl.handle.net/1842/6265.

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Atherosclerosis is one of the main diseases responsible for the high global mortality rate involving heart and blood vessel disorders. The build-up of fatty materials in the inner wall of the human artery prevents sufficient oxygen and nutrients reaching the organs of the body. Atherosclerosis is a chronic, long term condition, which develops and progresses over time; however, the disease does not present any symptoms until an advanced stage is reached, which results in potential permanent debility and sometimes sudden death. This thesis is concerned with the progression of atherosclerosis in
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Libros sobre el tema "Cell migration"

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Guan, Jun-Lin. Cell Migration. Humana Press, 2004. http://dx.doi.org/10.1385/1592598609.

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Wells, Claire M., and Maddy Parsons, eds. Cell Migration. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-207-6.

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Gautreau, Alexis, ed. Cell Migration. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7701-7.

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Filippi, Marie-Dominique, and Hartmut Geiger, eds. Stem Cell Migration. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-145-1.

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Margadant, Coert, ed. Cell Migration in Three Dimensions. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2887-4.

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Frank, Entschladen, and Zänker Kurt S, eds. Cell migration: Signalling and mechanisms. Karger, 2010.

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D'Ambrosio, Daniele, and Francesco Sinigaglia. Cell Migration in Inflammation and Immunity. Humana Press, 2003. http://dx.doi.org/10.1385/1592594352.

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Doris, Wedlich, ed. Cell migration in development and disease. Wiley-VCH, 2005.

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1949-, Husband Alan J., ed. Migration and homing of lymphoid cells. CRC Press, 1988.

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Tazawa, M. Cell Dynamics: Cytoplasmic Streaming Cell Movement-Contraction and Migration Cell and Organelle Division Phototaxis of Cell and Cell Organelle. Springer Vienna, 1989.

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Capítulos de libros sobre el tema "Cell migration"

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Rovensky, Yury A. "Cell Migration." In Adhesive Interactions in Normal and Transformed Cells. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-304-2_6.

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Seynhaeve, Ann L. B., and Timo L. M. ten Hagen. "An In Vivo Model to Study Cell Migration in XYZ-T Dimension Followed by Whole-Mount Re-evaluation." In Cell Migration in Three Dimensions. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2887-4_19.

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AbstractCell migration is a very dynamic process involving several chemical as well as biological interactions with other cells and the environment. Several models exist to study cell migration ranging from simple 2D in vitro cultures to more demanding 3D multicellular assays, to complex evaluation in animals. High-resolution 4D (XYZ, spatial + T, time dimension) intravital imaging using transgenic animals with a fluorescent label in cells of interest is a powerful tool to study cell migration in the correct environment. Here we describe an advanced dorsal skinfold chamber model to study endot
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Twyman, R. M. "Germ-cell migration." In BIOS Instant Notes in Developmental Biology. Taylor & Francis, 2023. http://dx.doi.org/10.1201/9781003416371-26.

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Preziosi, Luigi. "Cell Migration, Biomechanics." In Encyclopedia of Applied and Computational Mathematics. Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-540-70529-1_69.

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Moissoglu, Konstadinos, Stephen J. Lockett, and Stavroula Mili. "Visualizing and Quantifying mRNA Localization at the Invasive Front of 3D Cancer Spheroids." In Cell Migration in Three Dimensions. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2887-4_16.

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AbstractLocalization of mRNAs at the front of migrating cells is a widely used mechanism that functionally supports efficient cell movement. It is observed in single cells on two-dimensional surfaces, as well as in multicellular three-dimensional (3D) structures and in tissue in vivo. 3D multicellular cultures can reveal how the topology of the extracellular matrix and cell-cell contacts influence subcellular mRNA distributions. Here we describe a method for mRNA imaging in an inducible system of collective cancer cell invasion. MDA-MB-231 cancer cell spheroids are embedded in Matrigel, induce
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Bradbury, Joshua J., Holly E. Lovegrove, Marta Giralt-Pujol, and Shane P. Herbert. "Analysis of mRNA Subcellular Distribution in Collective Cell Migration." In Cell Migration in Three Dimensions. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2887-4_22.

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AbstractThe movement of groups of cells by collective cell migration requires division of labor between group members. Therefore, distinct cell identities, unique cell behaviors, and specific cellular roles are acquired by cells undergoing collective movement. A key driving force behind the acquisition of discrete cell states is the precise control of where, when, and how genes are expressed, both at the subcellular and supracellular level. Unraveling the mechanisms underpinning the spatiotemporal control of gene expression in collective cell migration requires not only suitable experimental m
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Svoren, Martin, Elena Camerini, Merijn van Erp, Feng Wei Yang, Gert-Jan Bakker, and Katarina Wolf. "Approaches to Determine Nuclear Shape in Cells During Migration Through Collagen Matrices." In Cell Migration in Three Dimensions. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2887-4_7.

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AbstractFibrillar collagen is an abundant extracellular matrix (ECM) component of interstitial tissues which supports the structure of many organs, including the skin and breast. Many different physiological processes, but also pathological processes such as metastatic cancer invasion, involve interstitial cell migration. Often, cell movement takes place through small ECM gaps and pores and depends upon the ability of the cell and its stiff nucleus to deform. Such nuclear deformation during cell migration may impact nuclear integrity, such as of chromatin or the nuclear envelope, and therefore
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Brüning-Richardson, A., and C. Kirby. "Cell Migration in Cancer; Cell Migration in 2D and 3D." In Learning Materials in Biosciences. Springer Nature Switzerland, 2024. http://dx.doi.org/10.1007/978-3-031-64532-7_5.

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Jacobs, Kathryn A., and Julie Gavard. "3D Endothelial Cell Migration." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7701-7_6.

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Prahl, Louis S., and David J. Odde. "Modeling Cell Migration Mechanics." In Advances in Experimental Medicine and Biology. Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-95294-9_9.

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Actas de conferencias sobre el tema "Cell migration"

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Grivey, Adrien, Kevin Cinglant, Fabrice Comblet, and Ali Khenchaf. "Range Cell Migration Processing Loss in Automotive Radar." In 2024 21st European Radar Conference (EuRAD). IEEE, 2024. http://dx.doi.org/10.23919/eurad61604.2024.10734886.

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Trusiak, Maciej, Piotr Arcab, Mikołaj Rogalski, Piotr Rogujski, and Luiza Stanaszek. "Multiplexed label-free high-throughput holographic lensless method for live cell migration sensing." In Computational Optical Sensing and Imaging. Optica Publishing Group, 2024. http://dx.doi.org/10.1364/cosi.2024.ctu1b.3.

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Cell migration plays crucial role in regeneration, morphogenesis and cancer metastasis. We present a novel hardware-software method for multiplexed (3-cameras) holographic lensless label-free full-culture live-cell quantitative migration sensing with single-cell sensitivity and sub-cellular motion precision.
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Maini, Philip K. "Modelling collective cell migration." In INTERNATIONAL CONFERENCE OF NUMERICAL ANALYSIS AND APPLIED MATHEMATICS ICNAAM 2019. AIP Publishing, 2020. http://dx.doi.org/10.1063/5.0026549.

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Na, Sungsoo. "Engineering Tools for Studying Coordination Between Biochemical and Biomechanical Activities in Cell Migration." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53709.

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Cell migration is achieved by the dynamic feedback interactions between traction forces generated by the cell and exerted onto the underlying extracellular matrix (ECM), and intracellular mechano-chemical signaling pathways, e.g., Rho GTPase (RhoA, Rac1, and Cdc42) activities [1,2,3]. These components are differentially distributed within a cell, and thus the coordination between tractions and mechanotransduction (i.e, RhoA and Rac1 activities) must be implemented at a precise spatial and temporal order to achieve optimized, directed cell migration [4,5]. Recent studies have shown that focal a
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Han, Sangyoon J., and Nathan J. Sniadecki. "Traction Forces During Cell Migration Predicted by the Multiphysics Model." In ASME 2011 International Mechanical Engineering Congress and Exposition. ASMEDC, 2011. http://dx.doi.org/10.1115/imece2011-63843.

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Cells rely on traction forces in order to crawl across a substrate. These traction forces come from dynamic changes in focal adhesions, cytoskeletal structures, and chemical and mechanical signals from the extracellular matrix. Several computational models have been developed that help explain the trajectory or accumulation of cells during migration, but little attention has been placed on traction forces during this process. Here, we investigated the spatial and temporal dynamics of traction forces by using a multiphysics model that describes the cycle of steps for a migrating cell on an arra
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Krishnamoorthy, Srikumar, and Changxue Xu. "Fabrication of a Graded Micropillar Surface for Guided Cell Migration." In ASME 2020 15th International Manufacturing Science and Engineering Conference. American Society of Mechanical Engineers, 2020. http://dx.doi.org/10.1115/msec2020-8332.

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Abstract The migration of cells is caused by the interaction of cells and the local microenvironment around them, such as changes in stiffness, chemical gradients etc. The local topography of substrates in contact with cells is a key factor that regulates the migration of cells. The interaction between the topography of the substrate and cells is crucial for the understanding of tissue development and regeneration. In this paper, the fabrication of a graded micropillar substrate for studying topography-based cell migration is described in detail. The fabrication protocol comprises of the utili
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Monteiro, Gary A., Harini G. Sundararaghavan, Anthony V. Fernandes, and David I. Shreiber. "Enhancing Cell Migration in Collagen Gels by Modulating Collagen Adhesivity." In ASME 2008 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2008. http://dx.doi.org/10.1115/sbc2008-192945.

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The organized movement of cells is critical during tissue morphogenesis and wound healing. While different tissue cells use distinct mechanisms for migration, the underlying biophysical balance of adhesive and tractional forces for effective migration is similar. The extracellular matrix provides the structural framework through which a cell can migrate. In particular, collagen is an abundant and ubiquitous ECM protein that supports cell migration. The excellent biocompatibility and physiological relevance of collagen have made it a primary material for tissue engineered regenerative therapies
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Zielinski, Rachel, Cosmin Mihai, and Samir Ghadiali. "Multi-Scale Modeling of Cancer Cell Migration and Adhesion During Epithelial-to-Mesenchymal Transition." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53511.

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Cancer is a leading cause of death in the US, and tumor cell metastasis and secondary tumor formation are key factors in the malignancy and prognosis of the disease. The regulation of cell motility plays an important role in the migration and invasion of cancer cells into surrounding tissues. The primary modes of increased motility in cancerous tissues may include collective migration of a group of epithelial cells during tumor growth and single cell migration of mesenchymal cells after detachment from the primary tumor site [1]. In epithelial cancers, metastasizing cells lose their cell-cell
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Kraning-Rush, Casey M., Shawn P. Carey, and Cynthia A. Reinhart-King. "Molded Collagen Microchannels for the Study of Cancer Cell Invasion." In ASME 2012 10th International Conference on Nanochannels, Microchannels, and Minichannels collocated with the ASME 2012 Heat Transfer Summer Conference and the ASME 2012 Fluids Engineering Division Summer Meeting. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/icnmm2012-73093.

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Metastasis is the cause of 90% of cancer-related deaths and yet the precise mechanism of metastasis is poorly understood[1]. To metastasize, cells break free from the primary tumor, migrate through the surrounding tissue, and enter the vascular system to move to a secondary site. To migrate through the stroma, cell can both degrade the tissue and use physical force to move the tissue from its path. However, the relative roles of matrix degradation and cellular force are not well-understood. Previous work has shown that as cell move through the matrix, they create channels that other cells can
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Ohashi, Toshiro, and Akito Sugawara. "Traction Force Measurement During Collective Cell Migration Measured by Multichannel Micropillar Device." In ASME 2013 11th International Conference on Nanochannels, Microchannels, and Minichannels. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/icnmm2013-73163.

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Cell migration is essential for a variety of biological and pathological processes such as wound healing, inflammation and tumor metastasis. However, the mechanical environment within a group of cells during collective migration has not been well characterized. In this study, a polydimethylsiloxane (PDMS) multichannel device was fabricated using standard photolithography and soft lithography techniques and was used to monitor cellular traction forces during migration. A migration rate of 5.7 μm/h was measured in microchannels and leading cells in the moving front of the migration generated tra
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Informes sobre el tema "Cell migration"

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Gonzalez-Nieves, Reyda. Regulation of Cell Migration in Breast Cancer. Defense Technical Information Center, 2011. http://dx.doi.org/10.21236/ada553111.

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Cutler, Mary L. Targeting Epithelial Cell Migration to Accelerate Wound Healing. Defense Technical Information Center, 2010. http://dx.doi.org/10.21236/ada552380.

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Cutler, Mary. Targeting Epithelial Cell Migration to Accelerate Wound Healing. Defense Technical Information Center, 2012. http://dx.doi.org/10.21236/ada627078.

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Paul, Satashree. Flavivirus and its Threat. Science Repository, 2021. http://dx.doi.org/10.31487/sr.blog.30.

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A number of studies found that the virus can activate the endothelial cells and affect the structure and function of the blood?brain barrier, promoting immune cell migration to benefit the virus nervous system target cells infected by flaviviruses.
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Chanvorachote, Pithi. Roles of nitric oxide, reactive oxygen species, and their derivatives in regulation of lung cancer cell metastasis. Chulalongkorn University, 2013. https://doi.org/10.58837/chula.res.2013.28.

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The capability of cancer cells to resist to anoikis, migrate and invade surrounding tissues is associated with high metastatic potential and advanced stage of cancers. Recently, caveolin-1 (Cav-1) protein has garnered increased attention in implicating the aggressive behavior of cancer cells. We demonstrate herein that nitric oxide and hydrogen peroxide play a role in inhibiting anoikis process of lung cancer cells via caveolin-1 dependent mechanism. The Cav-1 function in inhibition of anoikis was demonstrated to be cause through Mcl-1 dependent mechanism. The present study demonstrated that C
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Flopper, George E., and Jr. Cell Migration as a Therapeutic Target in Malignant Breast Cancer. Defense Technical Information Center, 2001. http://dx.doi.org/10.21236/ada396961.

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Plopper, George E., and Jr. Cell Migration as a Therapeutic Target in Malignant Breast Cancer. Defense Technical Information Center, 2002. http://dx.doi.org/10.21236/ada409486.

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Plopper, George E. Cell Migration as a Therapeutic Target in Malignant Breast Cancer. Defense Technical Information Center, 1999. http://dx.doi.org/10.21236/ada390932.

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Plopper, George E., and Jr. Cell Migration as a Therapeutic Target in Malignant Breast Cancer. Defense Technical Information Center, 2000. http://dx.doi.org/10.21236/ada392816.

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Haugh, Jason M. Integration of Soluble and Adhesive Gradient Signals in Directed Cell Migration. Defense Technical Information Center, 2006. http://dx.doi.org/10.21236/ada467054.

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