Bibliografías temáticas / Denaturing gradient gel electrophoresis

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Literatura académica sobre el tema "Denaturing gradient gel electrophoresis"

Autor: Grafiati
Publicado: 4 de junio de 2021
Última modificación: 31 de enero de 2023

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Artículos de revistas sobre el tema "Denaturing gradient gel electrophoresis"

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Hovig, Eivind, Birgitte Smith-Sørensen, Anton Brøgger, and Anne-Lise Børresen. "Constant denaturant gel electrophoresis, a modification of denaturing gradient gel electrophoresis, in mutation detection." Mutation Research Letters 262, no. 1 (January 1991): 63–71. http://dx.doi.org/10.1016/0165-7992(91)90108-g.

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Choe, Myeongeun, Sung-Jun Hong, Jong-Hui Lim, Yunyoung Kwak, Chang-Gi Back, Hee-Young Jung, In-Jung Lee, and Jae-Ho Shin. "Korean Paddy Soil Microbial Community Analysis Method Using Denaturing Gradient Gel Electrophoresis." Journal of Applied Biological Chemistry 56, no. 2 (June 30, 2013): 95–100. http://dx.doi.org/10.3839/jabc.2013.016.

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Cremonesi, Laura, Paola Carrera, Antonella Fumagalli, Sabrina Lucchiari, Elena Cardillo, Maurizio Ferrari, Sabina Carla Righetti, Franco Zunino, Pier Giorgio Righetti, and Cecilia Gelfi. "Validation of Double Gradient Denaturing Gradient Gel Electrophoresis through Multigenic Retrospective Analysis." Clinical Chemistry 45, no. 1 (January 1, 1999): 35–40. http://dx.doi.org/10.1093/clinchem/45.1.35.

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Abstract Among established techniques for the identification of either known or new mutations, denaturing gradient gel electrophoresis (DGGE) is one of the most effective. However, conventional DGGE is affected by major drawbacks that limit its routine application: the different denaturant gradient ranges and migration times required for different DNA fragments. We developed a modified version of DGGE for high-throughput mutational analysis, double gradient DGGE (DG-DGGE), by superimposing a porous gradient over the denaturant gradient, which maintains the zone-sharpening effect even during le
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Adil, Essahale. "Corrective Measures of Denaturing Gradient Gel Electrophoresis Limitations." Journal of Environmental Science and Technology 8, no. 1 (December 15, 2014): 1–12. http://dx.doi.org/10.3923/jest.2015.1.12.

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Radojkovic, Dragica, and Jelena Kušic. "Silver Staining of Denaturing Gradient Gel Electrophoresis Gels." Clinical Chemistry 46, no. 6 (June 1, 2000): 883–84. http://dx.doi.org/10.1093/clinchem/46.6.883.

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Fodde, Riccardo, and Monique Losekoot. "Mutation detection by denaturing gradient gel electrophoresis (DGGE)." Human Mutation 3, no. 2 (1994): 83–94. http://dx.doi.org/10.1002/humu.1380030202.

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Valášková, V., and P. Baldrian. "Denaturing gradient gel electrophoresis as a fingerprinting method for the analysis of soil microbial communities." Plant, Soil and Environment 55, No. 10 (October 21, 2009): 413–23. http://dx.doi.org/10.17221/132/2009-pse.

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In soil microbial ecology, the effects of environmental factors and their gradients, temporal changes or the response to specific experimental treatments of microbial communities can only be effectively analyzed using methods that address the structural differences among whole communities. Fingerprinting methods are the most appropriate technique for this task when multiple samples must be analyzed. Among the methods currently used to compare microbial communities based on nucleic acid sequences, the techniques based on differences in the melting properties of double-stranded molecules, denatu
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Willame, Raphaël Boutte, Stana Grubisic, Pierre Balthasart, Annick Wilmotte, and Lucien Hoffmann. "Seasonal cyanobacterial dynamics in a mesoeutrophic reservoir: microscopic counts and DGGE (Denaturing Gradient Gel Electrophoresis)." Algological Studies 129 (September 1, 2009): 71–94. http://dx.doi.org/10.1127/1864-1318/2009/0129-0071.

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Nakatsu, C. H. "Soil Microbial Community Analysis Using Denaturing Gradient Gel Electrophoresis." Soil Science Society of America Journal 71, no. 2 (March 2007): 562–71. http://dx.doi.org/10.2136/sssaj2006.0080.

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Shi, L., G. Yan, Y. Fu, L. Ma, A. Penfornis, and D. Faustman. "Human TAP1 polymorphisms detected by denaturing gradient gel electrophoresis." Tissue Antigens 49, no. 4 (April 1997): 421–26. http://dx.doi.org/10.1111/j.1399-0039.1997.tb02772.x.

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Tesis sobre el tema "Denaturing gradient gel electrophoresis"

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Cadiou, Helene. "Evaluation of denaturing gradient gel electrophoresis (DGGE) as a method of mutation detection." Thesis, University College London (University of London), 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.418093.

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Le, Riche Mia. "Lipoprotein X : biochemical predictors and detection by non-denaturing polyacrylamide gradient gel electrophoresis." Thesis, Stellenbosch : Stellenbosch University, 2007. http://hdl.handle.net/10019.1/18218.

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Assignment (MMed)--University of Stellenbosch, 2007.<br>ENGLISH ABSTRACT: Lipoprotein X (LpX) is an abnormal cholesterol-containing particle that may be present in the serum of subjects with cholestasis, lecithin:cholesterol acyltransferase (LCAT) deficiency and parenteral nutrition. The biochemistry, metabolism, clinical significance and laboratory analysis of LpX is discussed in this study. This laboratory-based project investigated icteric samples received at the Chemical Pathology laboratory, Tygerberg Hospital, for serum predictors of LpX and the use of a modified non-denaturing polyacryl
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Surridge, Angela Karen Joanna. "Denaturing gradient gel electrophoresis characterisation of microbial communities in polycyclic aromatic hydrocarbon and polychlorinated biphenyl contaminated soil." Thesis, Pretoria : [s.n.], 2007. http://hdl.handle.net/2263/25070.

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Fossil fuels are currently the primary industrial energy source on Earth. They are principally composed of complex hydrocarbons in either long-chain or cyclic conformation. Industrial use of petroleum, diesel, oil, tar and other coal-derived products inevitably leads to pollution of the environment. The most serious pollution is caused by polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) that are not easily removed from soil after a spill. Long-chain and cyclic conformation makes fossil fuel hydrocarbons difficult to break down. However, certain free-living soil micr
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Ellwood-Thompson, Rhianedd Eleri. "Occurrence and transmission of Wolbachia endosymbionts in the oak gall wasp community : application of denaturing gradient gel electrophoresis." Thesis, Cardiff University, 2004. http://orca.cf.ac.uk/55379/.

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Eight Wolbachia variants were identified in the wasp community. Identical Wolbachia variants were identified in inquiline and parasitoid wasp species suggesting that horizontal transmission of Wolbachia occurs in this community
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Strandgren, Charlotte. "Studies of the Diversity of Lactobacillus spp. in Fecal Samples Using PCR and Denaturing Gradient Gel Electrophoresis." Thesis, Linköpings universitet, Institutionen för klinisk och experimentell medicin, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-15560.

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Allergic diseases, for example asthma and eczema, are nowadays considered belonging to the most common chronic diseases amongst children in the West, but the cause for this increase in allergy prevalence is unknown. Since studies have indicated a connection between children's exposure of microorganisms during infancy and risk of developing allergic disease, it is suggested that this exposure is a crucial factor in question of allergy development or not. Other studies have established differences in microflora composition between healthy children and children with allergic disease, and several
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Keyser, Maricel. "PCR detection, denaturing gradient gel electrophoresis (DGGE) fingerprinting and identification of the microbial consortium in different types of UASB granules." Thesis, Link to online version, 2006. http://hdl.handle.net/10019/558.

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Venter, Leandra. "Presence of potentially pathogenic heterotrophic plate count (HPC) bacteria occurring in a drinking water distribution system in the North-West Province, South Africa / by Leandra Venter." Thesis, North-West University, 2010. http://hdl.handle.net/10394/4380.

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There is currently growing concern about the presence of heterotrophic plate count (HPC) bacteria in drinking water. These HPC may have potential pathogenic features, enabling them to cause disease. It is especially alarming amongst individuals with a weakened immune system. South Africa, the country with the highest incidents of HIV positive individuals in the world, mainly uses these counts to assess the quality of drinking water in terms of the number of micro-organisms present in the water. These micro-organisms may be present in the bulk water or as biofilms adhered to the surfaces of a d
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Lindelof, Kara L. "Contribution of Biosolids-derived Bioaerosols to the Airborne Microbial Population." University of Toledo / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1302299544.

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PRUDEN, AMY J. "BIODEGRADATION OF METHYL TERT -BUTYL ETHER." University of Cincinnati / OhioLINK, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1027943573.

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Van, Niekerk Bertina Freda. "Functional and structural diversity of the microbial communities associated with the use of Fischer–Tropsch GTL Primary Column Bottoms as process cooling water / van Niekerk B.F." Thesis, North-West University, 2011. http://hdl.handle.net/10394/7284.

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Despite emerging water shortages, most water is only used once, and often with low efficiency. However, with appropriate treatment, water can be re–used to reduce the demand on freshwater sources. The Department of Water Affairs, South Africa, promotes industries to reduce discharges into water resources in order to sustain an overall good water quality of all water systems. All of this ultimately leads to industries striving towards zero effluent discharge. Primary Column Bottoms (PCBs) is a wastewater stream derived from the Fischer–Tropsch Gas to Liquid process and consists mainly of organi
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Capítulos de libros sobre el tema "Denaturing gradient gel electrophoresis"

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Netzer, Kai-Olaf. "Denaturing Gradient Gel Electrophoresis." In Techniques in Molecular Medicine, 86–95. Berlin, Heidelberg: Springer Berlin Heidelberg, 1999. http://dx.doi.org/10.1007/978-3-642-59811-1_6.

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Roelfsema, Jeroen H., and Dorien J. M. Peters. "Denaturing Gradient Gel Electrophoresis (DGGE)." In Springer Protocols Handbooks, 107–15. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-375-6_8.

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Strathdee, Fiona, and Andrew Free. "Denaturing Gradient Gel Electrophoresis (DGGE)." In Methods in Molecular Biology, 145–57. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-565-1_9.

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Roelfsema, Jeroen H., and Dorien J. M. Peters. "Denaturing Gradient Gel Electrophoresis (DGGE)." In Medical Biomethods Handbook, 79–86. Totowa, NJ: Humana Press, 2005. http://dx.doi.org/10.1385/1-59259-870-6:079.

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Fodde, Riccardo, and Monique Losekoot. "Mutation Analysis by Denaturing Gradient Gel Electrophoresis (DGGE)." In Technologies for Detection of DNA Damage and Mutations, 253–65. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-0301-3_19.

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Knapp, Leslie A. "Single Nucleotide Polymorphism Screening with Denaturing Gradient Gel Electrophoresis." In Methods in Molecular Biology, 137–51. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-411-1_8.

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Green, S. J., M. B. Leigh, and J. D. Neufeld. "Denaturing Gradient Gel Electrophoresis (DGGE) for Microbial Community Analysis." In Handbook of Hydrocarbon and Lipid Microbiology, 4137–58. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-540-77587-4_323.

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Green, Stefan J., Mary Beth Leigh, and Josh D. Neufeld. "Denaturing Gradient Gel Electrophoresis (DGGE) for Microbial Community Analysis." In Springer Protocols Handbooks, 77–99. Berlin, Heidelberg: Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/8623_2015_99.

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Lerman, Leonard S. "Electrophoresis of DNA in Denaturing Gradient Gels." In Genetic Engineering, 221–40. Boston, MA: Springer US, 1986. http://dx.doi.org/10.1007/978-1-4615-9456-7_12.

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Jasser, Iwona, Aleksandra Bukowska, Jean-Francois Humbert, Kaisa Haukka, and David P. Fewer. "Analysis of Toxigenic Cyanobacterial Communities through Denaturing Gradient Gel Electrophoresis." In Molecular Tools for the Detection and Quantification of Toxigenic Cyanobacteria, 263–75. Chichester, UK: John Wiley & Sons, Ltd, 2017. http://dx.doi.org/10.1002/9781119332169.ch9.

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Actas de conferencias sobre el tema "Denaturing gradient gel electrophoresis"

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Apperson, Charles S. "Comparison of microbial communities in monogyne and polygyne Solenopsis invicta colonies by denaturing gradient gel electrophoresis." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.112033.

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Arisuryanti, Tuty, Nu-Wei Vivian Wei, and Chris Austin. "Molecular evidence for determination cryptic species of Indonesian swamp eel populations using denaturing gradient gel electrophoresis (DGGE)." In TOWARDS THE SUSTAINABLE USE OF BIODIVERSITY IN A CHANGING ENVIRONMENT: FROM BASIC TO APPLIED RESEARCH: Proceeding of the 4th International Conference on Biological Science. Author(s), 2016. http://dx.doi.org/10.1063/1.4953534.

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Yang, Lijuan, Mengchun Gao, Fangyuan Liang, and Chao Qin. "Microbial Community Analysis During Composting of Digested Sludge and Sawdust by Using Quinone Profile and Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis." In 2009 3rd International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2009. http://dx.doi.org/10.1109/icbbe.2009.5163049.

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Dorfman, Kevin D., and Nabil Laachi. "Band Broadening During High-Throughput Mutation Detection in Microchannels." In ASME 2007 5th International Conference on Nanochannels, Microchannels, and Minichannels. ASMEDC, 2007. http://dx.doi.org/10.1115/icnmm2007-30051.

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Cycling temperature gradient electrophoresis represents a promising method for performing high-throughput DNA mutation detection in a microfluidic platform. Sweeping the temperature between an “all denatured” and “all annealed” state eliminates difficulties introduced by the low thermal mass of the system, while still preserving a mobility difference between the wild type and mutant alleles. We describe a theoretical analysis of this method of mutation detection, based on a multiple-time scales analysis that is valid when the DNA experience many temperature cycles before reaching the detector
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Farache Trajano, Luiza, Rebecca Moore, and Quentin Sattentau. "The Presence of Chemical Cross-Linking Stabilises HIV-1 Envelope Glycoprotein Trimer Antigens in a Model of Intramuscular Immunisation." In Building Bridges in Medical Science 2021. Cambridge Medicine Journal, 2021. http://dx.doi.org/10.7244/cmj.2021.03.001.4.

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Background: The HIV-1 envelope glycoprotein (Env) is the target of antigen design for antibody- based vaccination. In 2019, four trimeric Env vaccines entered an experimental trial: ConM, ConS, and their cross-linked counterparts. The trimers were formulated with MPLA adjuvant. Studies have demonstrated that adjuvants trigger neutrophil infiltration. Neutrophils activate and degranulate releasing proteases, namely elastase and cathepsinG. Aims: To assess the stability and immunogenicity of these vaccines in the presence of adjuvant- recruited neutrophils and their proteolytic enzymes. Methods:
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Electricwala, A., and T. Atkinson. "TANDEM PURIFICATION OF TISSUE PLASMINOGEN ACTIVATOR BY METAL CHELATE AND AFFINITY CHROMATOGRAPHY." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644831.

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A tandem purification procedure was developed by a combination of metal chelate and affinity chromatography. The conditioned medium from different cell lines producing tissue plasminogen activator was first chromatographed on a zinc chelate-agarose column equilibrated with low ionic strength buffer. After thorough washing, the column was connected to a lysine-agarose column, previously equilibrated with the same buffer. Tissue plasminogen activator was eluted from the zinc chelate column by a gradient of imidazole and the effluent was allowed to flow through lysine-agarose matrix. The two colu
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Kempfer, A. C., N. Maugeri, C. Farías, E. Bermejo, M. Gimeno, and M. Lazzari. "PURIFICATION AND PARTIAL CHARACTERIZATION OF A BIOACTIVE SUBSTANCE FROM RAT'S VESSEL WALL INDEPENDENT OF PROSTACYCLIN PRODUCTION." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643362.

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Our previous observations provided evidence that a bioactive substance (BAS) with inhibitory effect on platelet aggregation and with inotropic activity on smooth muscle preparations is present in aortic wall of rats treated previously with indomethacin. The ability to inhibit platelet aggregation was used for monitoring its purification and partial characterization. The original sample was extracted by rinsing aortic rings (1.5 mg dried rings) in buffer Krebs (300 μl) for 40 minutes at room temperature. The substance was purified by gel filtration (Bio-gel P-30, Sephadex G-100, Sephadex G-75)
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Heeb, M. J., D. F. Mosher, and J. H. Griffin. "PROTEIN C IS ACTIVATED AND GIVES 110,000 MW COMPLEXES AND PROTEIN S IS CLEAVED AND DECREASED _IN VIVO IN PATIENTS WITH INTRAVASCULAR COAGULATION (DIC)." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644696.

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Immunoblotting studies using denaturing and nondenaturing polyacrylamide gel electrophoresis conditions were performed on 100 plasmas from 88 patients with suspected DIC, in order to determine whether the anticoagulant regulatory proteins C and S (PC and PS) cure altered in vivo during DIC. 70 of these plasmas from 65 patients contained 5-35% of PC antigen in the form of activated protein C (APC) complexed with inhibitor(s).24 normal plasmas showed no detectable APC-inhibitor complexes.The complexes in DIC plasmas had a MW of 110 K on SDS-PAGE, as did complexes formed when APC was incubated wi
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Jørgensen, M. "HEPARIN INDEPENDENT PURIFICATION OF ANTITHROMBIN III (AT III) BY IMMUNO-AFFINITY CHROMATOGRAPHY RESULTING IN A FUNCTIONALLY INTACT MOLECULE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643682.

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Previous methods for purification of AT III are based on its heparin-binding capacity. However, in congenital AT III deficiency abnormal inhibitor molecules with impaired binding of heparin and/or thrombin has been reported. The aim of the present study was to develop a purification method based on immuno-affinity chromatography, and thus independent of the heparin binding capacity.Rabbits were immunized with human AT III purified by a three-step procedure involving dextran sulphate precipitation, affinity chromatography on heparin-Sepharose and ion-exchange chromatography on DEAE-Sephadex A-5
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Meijers, Joost C. M., Pim N. M. Tijburg та Bonno N. Bouma. "INHIBITION OF HUMAN BLOOD COAGULATION FACTOR Xa BY α2-MACROGLO-BULIN". У XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643838.

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The inactivation of activated factor X (factor Xa) by α2 macroglobulin (α2M) was studied. Irreversible inhibition was observed with the initial formation of a reversible enzyme-inhibitor complex The secopd-order rate constant for the reaction was 8.4 × 104 M−1 min−1. The binding ratio was found to be 2 mol factor Xa/ mol α2M. Interaction of factor Xa with α2M resulted in the appearance of four thiolgroups/molecule α2. The apparent second-order rate constants for the appearance of thiolgroups were dependent on the factor Xa concentration. Sodium dodecyl sulphate gradient polyacrylamide gel elec
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