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Literatura académica sobre el tema "Minion"

Autor: Grafiati

Publicado: 4 de junio de 2021

Última modificación: 14 de febrero de 2022

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Artículos de revistas sobre el tema "Minion"

Rusk, Nicole. "MinION takes center stage". Nature Methods 12, n.º 1 (30 de diciembre de 2014): 12. http://dx.doi.org/10.1038/nmeth.3244.

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Rowan, Chris. "Meet my lab minion". Nature 450, n.º 7167 (noviembre de 2007): 316. http://dx.doi.org/10.1038/nj7167-316c.

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Andracki, Thaddeus. "Minion by John David Anderson". Bulletin of the Center for Children's Books 68, n.º 1 (2014): 5. http://dx.doi.org/10.1353/bcc.2014.0706.

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Minion, F. ,. Chris. "Molecular pathogenesis of mycoplasma animal respiratory pathogens". Frontiers in Bioscience 7, n.º 1-3 (2002): d1410. http://dx.doi.org/10.2741/minion.

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Tafess, Ketema, Timothy Ting Leung Ng, Hiu Yin Lao, Kenneth Siu Sing Leung, Kingsley King Gee Tam, Rahim Rajwani, Sarah Tsz Yan Tam et al. "Targeted-Sequencing Workflows for Comprehensive Drug Resistance Profiling of Mycobacterium tuberculosis Cultures Using Two Commercial Sequencing Platforms: Comparison of Analytical and Diagnostic Performance, Turnaround Time, and Cost". Clinical Chemistry 66, n.º 6 (2 de mayo de 2020): 809–20. http://dx.doi.org/10.1093/clinchem/hvaa092.

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Abstract Background The emergence of Mycobacterium tuberculosis with complex drug resistance profiles necessitates a rapid and comprehensive drug susceptibility test for guidance of patient treatment. We developed two targeted-sequencing workflows based on Illumina MiSeq and Nanopore MinION for the prediction of drug resistance in M. tuberculosis toward 12 antibiotics. Methods A total of 163 M. tuberculosis isolates collected from Hong Kong and Ethiopia were subjected to a multiplex PCR for simultaneous amplification of 19 drug resistance-associated genetic regions. The amplicons were then barcoded and sequenced in parallel on MiSeq and MinION in respective batch sizes of 24 and 12 samples. A web-based bioinformatics pipeline, BacterioChek-TB, was developed to translate the raw datasets into clinician-friendly reports. Results Both platforms successfully sequenced all samples with mean read depths of 1,127× and 1,649×, respectively. The variant calling by MiSeq and MinION could achieve 100% agreement if variants with an allele frequency of <40% reported by MinION were excluded. Both workflows achieved a mean clinical sensitivity of 94.8% and clinical specificity of 98.0% when compared with phenotypic drug susceptibility test (pDST). Turnaround times for the MiSeq and MinION workflows were 38 and 15 h, facilitating the delivery of treatment guidance at least 17–18 days earlier than pDST, respectively. The higher cost per sample on the MinION platform ($71.56) versus the MiSeq platform ($67.83) was attributed to differences in batching capabilities. Conclusion Our study demonstrates the interchangeability of MiSeq and MinION platforms for generation of accurate and actionable results for the treatment of tuberculosis.

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Agakhanov, M. M., E. A. Grigoreva, E. K. Potokina, P. S. Ulianich y Y. V. Ukhatova. "Genome assembly of Vitis rotundifolia Michx. using third-generation sequencing (Oxford Nanopore Technologies)". Proceedings on applied botany, genetics and breeding 182, n.º 2 (1 de julio de 2021): 63–71. http://dx.doi.org/10.30901/2227-8834-2021-2-63-71.

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The immune North American grapevine species Vitis rotundifolia Michaux (subgen. Muscadinia Planch.) is regarded as a potential donor of disease resistance genes, withstanding such dangerous diseases of grapes as powdery and downy mildews. The cultivar ‘Dixie’ is the only representative of this species preserved ex situ in Russia: it is maintained by the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) in the orchards of its branch, Krymsk Experiment Breeding Station. Third-generation sequencing on the MinION platform was performed to obtain information on the primary structure of the cultivar’s genomic DNA, employing also the results of Illumina sequencing available in databases. A detailed description of the technique with modifications at various stages is presented, as it was used for grapevine genome sequencing and whole-genome sequence assembly. The modified technique included the main stages of the original protocol recommended by the MinION producer: 1) DNA extraction; 2) preparation of libraries for sequencing; 3) MinION sequencing and bioinformatic data processing; 4) de novo whole-genome sequence assembly using only MinION data or hybrid assembly (MinION+Illumina data); and 5) functional annotation of the whole-genome assembly. Stage 4 included not only de novo sequencing, but also the analysis of the available bioinformatic data, thus minimizing errors and increasing precision during the assembly of the studied genome. The DNA isolated from the leaves of cv. ‘Dixie’ was sequenced using two MinION flow cells (R9.4.1).

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Lemon, Jamie K., Pavel P. Khil, Karen M. Frank y John P. Dekker. "Rapid Nanopore Sequencing of Plasmids and Resistance Gene Detection in Clinical Isolates". Journal of Clinical Microbiology 55, n.º 12 (11 de octubre de 2017): 3530–43. http://dx.doi.org/10.1128/jcm.01069-17.

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ABSTRACTRecent advances in nanopore sequencing technology have led to a substantial increase in throughput and sequence quality. Together, these improvements may permit real-time benchtop genomic sequencing and antimicrobial resistance gene detection in clinical isolates. In this study, we evaluated workflows and turnaround times for a benchtop long-read sequencing approach in the clinical microbiology laboratory using the Oxford Nanopore Technologies MinION sequencer. We performed genomic and plasmid sequencing of three clinical isolates with both MinION and Illumina MiSeq, using different library preparation methods (2D and rapid 1D) with the goal of antimicrobial resistance gene detection. We specifically evaluated the advantages of using plasmid DNA for sequencing and the value of supplementing MinION sequences with MiSeq reads for increasing assembly accuracy. Resequencing of three plasmids in a referenceKlebsiella pneumoniaeisolate demonstrated ∼99% accuracy of draft MinION-only assembly and >99.9% accuracy of assembly polished with MiSeq reads. Plasmid DNA sequencing of previously uncharacterized clinical extended-spectrum β-lactamase (ESBL)-producingEscherichia coliandK. pneumoniaeisolates using MinION allowed successful identification of antimicrobial resistance genes in the draft assembly corresponding to all classes of observed plasmid-based phenotypic resistance. Importantly, use of plasmid DNA enabled lower depth sequencing, and assemblies sufficient for full antimicrobial resistance gene annotation were obtained with as few as 2,000 to 5,000 reads, which could be acquired in 20 min of sequencing. With a MinION-only workflow that balances accuracy against turnaround time, full annotation of plasmid resistance gene content could be obtained in under 6 h from a subcultured isolate, less time than traditional phenotypic susceptibility testing.

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Sinha, Gunjan. "A New King and His Tiny Minion". Scientific American 275, n.º 2 (agosto de 1996): 24. http://dx.doi.org/10.1038/scientificamerican0896-24b.

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White, Ruby, Christophe Pellefigues, Franca Ronchese, Olivier Lamiable y David Eccles. "Investigation of chimeric reads using the MinION". F1000Research 6 (5 de mayo de 2017): 631. http://dx.doi.org/10.12688/f1000research.11547.1.

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Following a nanopore sequencing run of PCR products of three amplicons less than 1kb, an abundance of reads failed quality control due to template/complement mismatch. A BLAST search demonstrated that some of the failed reads mapped to two different genes -- an unexpected observation, given that PCR was carried out separately for each amplicon. A further investigation was carried out specifically to search for chimeric reads, using separate barcodes for each amplicon and trying two different ligation methods prior to sample loading. Despite the separation of ligation products, chimeric reads formed from different amplicons were still observed in the base-called sequence.The long-read nature of nanopore sequencing presents an effective tool for the discovery and filtering of chimeric reads. We have found that at least 1.7% of reads prepared using the Nanopore LSK002 2D Ligation Kit include post-amplification chimeric elements. This finding has potential implications for other amplicon sequencing technologies, as the process is unlikely to be specific to the sample preparation used for nanopore sequencing.

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White, Ruby, Christophe Pellefigues, Franca Ronchese, Olivier Lamiable y David Eccles. "Investigation of chimeric reads using the MinION". F1000Research 6 (16 de agosto de 2017): 631. http://dx.doi.org/10.12688/f1000research.11547.2.

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Following a nanopore sequencing run of PCR products of three amplicons less than 1kb, an abundance of reads failed quality control due to template/complement mismatch. A BLAST search demonstrated that some of the failed reads mapped to two different genes -- an unexpected observation, given that PCR was carried out separately for each amplicon. A further investigation was carried out specifically to search for chimeric reads, using separate barcodes for each amplicon and trying two different ligation methods prior to sample loading. Despite the separation of ligation products, chimeric reads formed from different amplicons were still observed in the base-called sequence. The long-read nature of nanopore sequencing presents an effective tool for the discovery and filtering of chimeric reads. We have found that at least 1.7% of reads prepared using the Nanopore LSK002 2D Ligation Kit include post-amplification chimeric elements. This finding has potential implications for other amplicon sequencing technologies, as the process is unlikely to be specific to the sample preparation used for nanopore sequencing.

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Tesis sobre el tema "Minion"

Karlsson, Katerina. "Minion- en ordningsam vän i tvättstugan". Thesis, Högskolan i Gävle, Akademin för teknik och miljö, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-16785.

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Minion- en ordningsam vän i tvättstugan. Tvättstugan. En samhällstjänst som alla tar för givet eller en arena för dåligt beteende. Vad den än är så är den stället där beteenden som fusk med städning samt stulna tvättider och kläder sker vilket kan mynna ut i riktigt allvarliga bråk som polisanmäls. Att ändra på tvättstugan och hålla ett vakande öga över den kostar pengar och det har inte alla. Under dessa förhållanden kommer problemen som påträffas där, bara att fortsätta att existera tills alla tvättstugor stängs. Forskning har visat att bilder på ögon motarbetar beteenden som orsakar problem i tvättstugan. De skapar en omedveten känsla av övervakning som korrigerar beteenden genom medvetenheten om det dåliga beteendet, eller av rädslan att bli påkommen och bestraffad. När bilder på ögon har en så stor effekt på oss kommer en interaktiv robot försedd med ögon, röst och en snäll personlighet att ha samma effekt samt förstärka känslan av övervakning. Minion är en liten interaktiv robot som håller tvättstugans besökare sällskap och blir dess beskyddare. Minions huvuduppgift är att påminna tvättstugans besökare att de aldrig är ensamma där och därmed motarbeta dåligt beteende som förstärks på avskilda platser. Minion lyckas med det genom att kommunicera, sjunga, dansa, äta ludd från torktumlaren och berätta lite olika saker om världen och sitt eget liv. Till och med i sin tystnad orsakar Minion känslan av övervakning för den som vistas i tvättstugan. Tyvärr är den existerande prototypen bara ett skal och inte en funktionell robot, därför förklarades Minions funktioner muntligt och med hjälp av bilder under användarstudien. Minion mottogs med spänning och godkännande och det visade sig att Minions alla funktioer är relevanta och uppfylls till fullo. Minion skulle kunna vidareutvecklas till andra områden av vardagslivet där en interaktiv robot kan vara användbar, som att förbättra livskvaliteten för människor i behov av en kamrat.
Minion- an orderly friend in the common laundry room The common laundry room. A service to society that everyone takes for granted or an arena for bad behavior. In either case, it is a place where cheating with cleaning up after yourself as well as hijacking appointments and stealing other people’s belongings can lead to really serious fights that are reported to the police. Changing the common laundry room and keeping a waking eye on it, costs a lot of money something that not everyone has access to. Under these circumstances, the problems found there will simply go on existing until all common laundry rooms are shut down. Research has proven that pictures of eyes can counter act behaviors similar to the ones causing trouble in the common laundry room. These pictures cause an unconscious feeling of being watched that will correct such behaviors either by creating consciousness of the bad behavior or the fear of being caught and then punished. As pictures of eyes have such a severe effect on us, an interactive robot with eyes, a voice and a kind personality will have the same effect as well as reinforce the feeling of being watched. Minion is a small interactive robot that keeps the common laundry room’s visitors company while being its patron. Minion’s main task is to remind the visitors that they are never there alone and can therefore counteract such bad behavior that is reinforced in secluded spaces. Minion succeeds in doing so by having the ability to communicate, sing, dance, eat fluff from the drier and recount facts about the world and its own life. Even while being silent it causes the same feeling of being watched for the ones being in the common laundry room with it. Unfortunately the existing prototype is that only of a shell and not a fully functional robot, as of which Minion’s functions had to be explained through pictures and reenactments during the user study that was made. Minion was embraced with excitement and approval and the case study proved that all of Minion’s functions are relevant as well as completely fulfilled. Minion could be further developed to other areas of daily life where an interactive robot could be of some use, such as in improving the quality of life for people in need of a companion.

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Baxter, John. "Evaluation of Oxford nanopore’s MinION : Use, functionality, and genome assembly". Thesis, Högskolan i Skövde, Institutionen för biovetenskap, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-17367.

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The rapid and reliable detection of pathogens is of utmost importance in healthcare settings to ensure the appropriate treatment thereby reducing morbidity and mortality for the patient. Current culturing, PCR based and NGS species detection methods are time consuming (Opota et al., 2015), limited in their detection (Buckley et al., 2015), or require specialist skills and are expensive (Basho and Eterovic., 2015). Oxford Technologies Nanopore devices could provide detailed genomic sequencing at a fraction of the cost and without the need for technical bioinformatic skills. This study evaluates the MinION device and analysis tools to suggest best practice. Classification and genotyping of 12 Klebsiella isolates were performed using EPI2ME automated workflows and manual de novo assembly. Automated workflows using raw MinION reads provided clinically relevant information identified in ~6hrs. Manual de novo assembly and analysis used hybrid, and single source data took >24hrs. The inclusion of MinION long reads overcome problems assembling short reads. Hybrid genomes provided the most contiguous and highly detailed contigs. MinION only read assemblies contained more errors but still identified similar genotypic findings. Automated workflows are rapid and require minimal bioinformatic know-how. There should be a dialogue between clinicians and bioinformaticians to develop bespoke analysis tools. Although challenges remain around compatible kits and vulnerable flowcells long read sequencing can be an effective tool for species detection and pathogen typing. Furthermore, hybrid assemblies have the potential to advance our genome detailing and discovery.

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Nilsson, Grimstad Kristoffer. "Evaluation of the Oxford Nanopore Minion for the identification and differentiation of MRSA and non-MRSA isolates". Thesis, Högskolan i Skövde, Institutionen för biovetenskap, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-18528.

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Staphylococcus aureus are bacterial pathogens causing infectious diseases. Methicillin-resistant S. aureus, or MRSA, carry the mecA or mecC genes generating resistance to β-lactam antibiotics. MRSA is problematic to treat and crucial to rapidly detect while current diagnostic workflows are time-consuming. DNA sequencing offers an alternative to existing methods. Illumina, the leading sequencing platform, is time-consuming and generates short reads. The Oxford Nanopore Technologies MinION presents rapid sequencing protocols, generating long reads at reduced accuracy. Here, the MinION device was evaluated to rapidly identify and differentiate between MRSA and non-MRSA isolates, with estimations on turn-around-times for clinical implementation.DNA extracted from 12 bacterial isolates were sequenced on the MinION. The automated workflow for raw MinION reads EPI2ME called taxonomy and resistance genes. MinION reads and corresponding Illumina reads were also assembled with Unicycler with short-read, long-read and hybrid assembly, and analyzed with the Center for Genomic Epidemiology bioinformatic tools. MinION and Illumina reads analyzed with the 1928 Diagnostics S. aureus pipelines were also compared. All tools correctly identified mecA in confirmed MRSA isolates, and mecC in an unconfirmed isolate. Similar results in terms of species, virulence and resistance genes were observed. Strain typing was problematic for MinION reads compared to Illumina, due to increased error rates. The minimum estimated turn-around-time for the MinION in this project, from library preparation, was approximately 6.5 hours to 7.5 hours per sample. Although further investigations are required, the MinION offers an intriguing alternative to current methods for identifying MRSA, aiding in rapid diagnosis and treatment.

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Hoskins, Sara Grace. "16th Century Cast-Bronze Ordnance at the Museu de Angra do Heroismo". Texas A&M University, 2003. http://hdl.handle.net/1969.1/556.

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Within the collections of the Museu de Angra do Heroismo (Terceira Island, Azores, Portugal) are nine cast bronze guns from the 16th century. Most were raised from the seafloor between the 1960s and 1990s, but this study comprises the first in-depth research into their design and manufacture. The importance of this kind of study lies in the fact that ordnance is commonly found on shipwrecks of this time. A greater knowledge of guns will help provide information about the ships from which they came. Careful documentation and study of the Museu de Angra cannon will add greatly to their value as museum exhibits, by allowing museum patrons to better understand where the guns came from, how they were cast, and why they were important. This documentation adds to our knowledge of Western European gunfounding technology during the sixteenth century, as four different countries commissioned the guns: Portugal, Spain, France, and England. With detailed documentation and publication, the Museu de Angra bronze guns can be added to the bibliography of ordnance of this period, which will aid future researchers who encounter similar pieces. The Museu de Angra bronze guns, as symbols of the military and naval power of the countries that commissioned them, were sent aboard ships, into the field, and mounted on fortress walls. Bronze guns of this time period are particularly important, as bronze was an expensive commodity, and the demand for ordnance was increasing rapidly. Countries developed more effective ways to make use of iron for the founding of guns, and the use of bronze became more symbolic of wealth. The information that each gun contains includes both the cutting-edge military technology of the time and the artistic statement of the founder. Some of the finest metalwork of the period was displayed in cast bronze guns, and due to the founding techniques, no two are the same, making each an important piece of history.

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Fruchard, Cécile. "Étude des chromosomes sexuels et du déterminisme du sexe chez les plantes : comparaison des systèmes Silene et Coccinia". Thesis, Lyon, 2018. http://www.theses.fr/2018LYSE1108/document.

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Bien que les sexes séparés (dioecie) soient plus rares que chez les animaux, ∼15 600 espèces dioiques ont évolué chez les angiospermes (∼6% de l'ensemble des espèces). La manière dont le sexe de ces plantes est contrôlé est une question centrale de la biologie végétale, mais également de l'agronomie car de nombreuses plantes cultivées sont des plantes dioiques (∼20% des espèces cultivées) mais dont un seul sexe (généralement les femelles) présente un intérêt agronomique. Pourtant, seulement trois gènes du déterminisme du sexe ont été identifiés à ce jour chez les plantes dioiques, chez le kaki, l'asperge et la fraise. La dioecie a vraisemblablement évolué plusieurs fois chez les angiospermes et il est possible que les gènes du déterminisme du sexe soient divers. Deux voies principales d'évolution vers la dioecie ont été identifiées. Les deux partent d'une espèce dont les fleurs sont hermaphrodites, le régime de reproduction ancestral chez les angiospermes, puis passent soit par un intermédiaire monoique (espèce avec des fleurs unisexuées mâles et femelles sur le même individu), soit par un intermédiaire gynodioique (espèce avec des femelles et des individus avec des fleurs hermaphrodites). Cette thèse a pour objet la comparaison de deux systèmes de plantes représentant ces deux voies. Chez Coccinia grandis, une cucurbitacée ayant également des chromosomes XY, l'évolution de la dioecie est passée par la monoecie. Chez Silene latifolia, une plante dioique bien étudiée avec des chromosomes sexuels XY, l'évolution de la dioecie s'est faite à partir de la gynodioecie. Trois gènes contrôlant la monoecie ont été identifiés chez le melon et il a été proposé que ces gènes soient les gènes du déterminisme dans les espèces dioiques proches du melon comme C. grandis. Nous avons donc opté pour une approche gène candidat dans cette espèce. Très peu de ressources génétiques et génomiques sont disponibles chez C. grandis, et nous avons choisi d'utiliser SEXDETector, une méthode probabiliste qui utilise des données RNA-seq pour génotyper des parents et leurs descendants, et qui infère les gènes lies au sexe sans génome de référence. Cette méthode m'a permis d'identifier 1 364 gènes présents sur les chromosomes sexuels de C. grandis. J'ai établi que les gènes differentiellement exprimés entre les sexes étaient plus abondants sur chromosomes sexuels que sur les autosomes. J'ai également observé des marques de la dégénérescence du chromosome Y chez cette plante, comme des diminutions d'expression ou des pertes de gènes. Enfin, mes résultats démontrent la présence de compensation de dosage chez C. grandis. Le test des gènes candidats est en cours. Chez S. latifolia, 3 grandes régions liées au déterminisme ont déjà été identifiées sur le chromosome Y. Pour identifier les gènes du déterminisme, nous avons choisi de séquencer ce chromosome. Le séquençage des chromosomes Y est encore un défi pour la génomique. La phase d'assemblage est très difficile à cause des répétitions présentes en grand nombre sur ces chromosomes. En conséquence, les séquences complètes de chromosome Y sont très rares, et principalement disponibles chez les animaux. Afin de minimiser les problèmes d'assemblage dus aux répétitions, nous avons utilisé des techniques dites de 3eme génération (avec de grandes lectures). J'ai moi-même généré des données MinION (Oxford Nanopore) à partir d'ADN de chromosome Y. L'assemblage a été réalisé en combinant des données Illumina, PacBio et MinION. Notre assemblage final fait une taille de 563 Mb pour un N50 de 6 114 pb, et contient 16 219 gènes annotés de novo
Although rarer than in animals, separate sexes (dioecy) have evolved in ∼15,600 angiosperm species (∼6% of all angiosperm species). How sex is controlled is a central question in plant sciences and also in agronomy as many crops are dioecious (∼20% of crops) with only one useful sex (usually female). Only three master sex-determining genes have been identified in dioecious plants so far, namely in persimmons, asparagus and strawberry. Dioecy likely evolved several times independently in angiosperms, suggesting that sex-determining genes are of diverse origins. Hermaphroditism is the predicted ancestral state of the angiosperm flower. Two main pathways have been identified that explain the evolution of hermaphroditism towards dioecy: either through a monoecious state (with both unisexual male and female flowers on the same individual) or a gynodioecious state (with females and individuals having hermaphroditic flowers). My aim is to compare two plant systems representing each one of these two pathways. In Coccinia grandis, a Cucurbitaceae with an XY chromosome system, dioecy evolved through monoecy. In Silene latifolia, a well-studied dioecious plant with XY sex chromosomes, dioecy evolved through gynodioecy. Three genes controlling monoecy have been identified in melon, and it was suggested that these genes act as sex-determining genes in closely related dioecious species such as C. grandis. I therefore chose a candidate gene approach in this species. Very few genetic and genomic data are available in C. grandis, and we chose to use SEX-DETector, a probabilistic method that uses RNA-seq data to genotype parents and their offspring, and infers sex-linked genes with no need for a reference genome. This method allowed me to identify 1,364 genes that are present on the sex chromosomes of C. grandis. I found that the sex chromosomes are enriched in sex-biasedgenes when compared to autosomes and I characterized Y chromosome degeneration in terms of decreased expression and gene loss. Finally, I showed that dosage compensation occurs in C. grandis. Testing for the three candidates genes is ongoing. In S. latifolia 3 regions involved in sex determination have already been identified on the Y chromosome. We chose to sequence this chromosome to identify sex-determining genes. The sequencing of Y chromosomes remains one of the greatest challenges of current genomics. The assembly step is very difficult because of their highly repeated content. Consequently, fully sequenced Y chromosomes are rare and mainly available for research in animals. To overcome the difficulty of assembling reads with many repeats, I used third generation sequencing (TGS, producing long reads). I produced a dataset using the Oxford Nanopore MinION sequencer with Y chromosome DNA. Assembling was performed using a combination of Illumina, MinION and PacBio sequencing data. The final assembly had a total length of 563 Mb with a scaffold N50 of 6,114 bp, and contained 16,219 de novo annotated genes

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Ulrich, Kristina. "Molecular epidemiological study on Infectious Pancreatic Necrosis Virus isolates from aquafarms in Scotland over three decades". Thesis, University of Stirling, 2018. http://hdl.handle.net/1893/28340.

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Introduction: RNA viruses are economically important pathogens of fish, and among these viruses, infectious pancreatic necrosis virus (IPNV) is of particular concern for the aquaculture industry, especially for farmed rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar). This non-enveloped aquatic virus, which was first isolated in the UK in 1971, belongs to the family of Birnaviridae and has a bi-segmented dsRNA genome of about 6kb. IPNV is classified in 6 genogroups with correspondence to 10 known serotypes and an additional proposed genogroup of marine aquabirnaviruses (MABV). IPNV causes high mortality in fry and a reduced mortality in adult fish, respectively. Fish, which survive, can become carriers and this can lead to a clinical outbreak by releasing infective material into water or by vertical transmission via oocytes, milt and seminal fluids. Methods: This project aimed at determining the phylogeny and genomic changes of IPNV in Scotland by whole genome sequence analysis of IPNV isolates (diagnostic TCID50 supernatants) spanning 3 decades since 1982, using next generation sequencing technology. Viral RNA of IPNV culture supernatant (CHSE-214 and TO cell culture) was processed for next generation sequencing on an Illumina MiSeq platform. Library preparation was performed using the Nextera XT DNA Library Kit, prior to sequencing according to the manufacturer's MiSeq Reagent Kit v3 (150cycles) protocol. To optimize whole genome next generation sequencing for IPNV, we compared two RNA processing protocols, the Glasgow (GLAP) and the Goettingen protocol (GOEP) with focus on missing terminal nucleotides after a de novo genome assembly. Sequences were used to determine the phylogeny and selection pressure on the genome as well as a possible virus-host adaptation. Results: The results showed that both protocols were able to give full length genomes as well as genomes with missing terminal nucleotides. The phylogenetic analysis of 57 sequenced IPVN isolates shows that 78.95 % of the isolates group within genogroup V, which includes serogroup Sp and 5.26 % within genogroup I which includes serogroup Ja. Segment A of 15.79 % of the isolate grouped within genogroup III, which includes serotype Ca1 and Te but only 7.02 % of the segment B isolates grouped in the genogroup III. The remaining 8.77 % of segment B groups within genogroup II, containing the Ab serotype. Previous research has shown that residue substitutions at positions 217 and 221 in the major capsid protein VP2 have an impact on the virulence of the virus, leading to different virulence types: virulent (T217, A221), low virulence (P217, A221), avirulent (T217, T221) and persistent (P217, T221). Whole genome sequence results show that 58.93 % of the sequenced isolates belong to the persistent, 32.14 % to the low virulent type, only one isolate was of a virulent type and 7.15 % had not virulence assigned amino acid compositions in positions 217 and 221. The selection pressure analysis showed that especially VP2 is experiencing selection pressure in the variable region. In the VP1 protein we see two sites under positive selection pressure within specific motifs. VP5 showed positive selected sites mostly within the truncated region of the protein. Other proteins showed no particular interesting sites of selection. The codon adaptation analysis showed highest adaptation index for VP2. Besides VP5, which had an CAI index below one, therefore showing negative adaptation, other IPNV proteins had an CAI of barely above the value of 1. The dinucleotide abundance, focussing on CpG, showed that CpG is underrepresented in segment A and B. Discussion Phylogenetic analysis of the sequenced IPNV strains shows separate clustering of different genogroups. Genetic reassortment is observed in segment B showing a grouping within genogroup III and II although the segment A of these isolates was grouping exclusively within III. We found that over 50 % of the isolates belong to the persistent and over 30 % to the low virulent type, assuming that due to not sterilising vaccination these types were selected in the vaccinated population. The results from the CAI calculations indicate an adaptation of IPNV to its host. Together with the findings that CpG is underrepresented in IPNV it suggests that this leads to an immune escape. Especially since the selection pressure analysis showed positive selection in VP2 within the virulence determination sites of the protein, indicating that IPNV "tries" to downregulate immune recognition. The prevalence of mostly persistent type of isolates indicates together with the assumption of adaptation and immune escape that IPNV is evolving with the host in order to ensure survival.

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Carboni, Lucrezia. "Superfici minime tra matematica e architettura". Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2018.

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Il problema di Plateau richiede di trovare tra tutte le superfici con un assegnato contorno quella di area minima. Le superfici soluzione di tale problema godono di una proprietà geometrica particolare: ogni punto della superficie ha curvature principali opposte. Le superfici che soddisfano quest’ultima proprietà si dicono superfici minime. Anche l’architettura ha fatto ampio uso di tali superfici, sia per la proprietà di assicurare un equilibrio stabile, sia perché permettono di contenere i costi in termini di minor materiale utilizzato, ma anche per la loro eleganza. In questo studio si riprendono alcuni dei concetti e dei principali risultati della geometria differenziale necessari per dare, nel secondo capitolo, una definizione rigorosa di superficie minima e di studiarne le principali proprietà. Successivamente, si studiano alcuni esempi di superfici minime, quali la superficie di Catalan, l’elicoide, unica superficie rigata non banale, e la catenoide, unica superficie minima di rotazione. Nel terzo capitolo vengono analizzati alcuni esempi di applicazione delle superfici minime in architettura.

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Stumbrytė, Eglė. "Minios fenomenas mene.Kūrybinis darbas: „KREPŠINIO SIRGALIŲ PORTRETAI“". Bachelor's thesis, Lithuanian Academic Libraries Network (LABT), 2012. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2012~D_20120702_104846-18714.

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Kintant laikams, minios elgesio, mąstymo bruožai iš esmės lieka nepakitę. Masė vis dar pasiduoda neracionaliems sprendimams, idėjoms, yra valdoma įvairiausių emocijų. Kritiškas individo protas yra nugalimas absurdiško fanatizmo išraiškų. Šiandieninėje Lietuvos kultūroje, vienas iš ryškiausių minią užliejančios karštligės atitikmenų - tai krepšinio sirgaliai, skanduojantys patriotinius šūkius, stabais verčiantys žaidėjus. Šiuolaikiniame mene taip pat nelieka nepastebėta ši tema, analizuojamas krepšinio santykis su visuomene, jo reikšmės kaita skirtingais laikmečiais. Darbo objektas. Trijų tapybos darbų ciklas – sirgalių portretai. Gilinimasis į darbo temą padėjo pažinti šį masininį reiškinį, minios bruožus ir apskritai sąmoningiau įvertinti, koks yra asmeninis santykis su masine karštlige.
As times change, the behaviour of mass and way of thinking remain the same. Mass is still relying on irrational solutions, ideas and is controlled by various emotions. Absurd fanatic manifestations overcome the critical mind of an individual. Nowadays, in the Lithuanian culture, one of the most prominent display of the mass fever can be recognised in basketball fans that cheer, shout patriotic slogans and tend to turn basketball players into idols. In contemporary art this theme is not overlooked – the relation between basketball and the society is constantly being analysed, as well as the change of its meaning in different periods of time. The object of work. The cycle of three painting works – portraits of fans. The theme of the work focuses on this mass phenomenon, analyses the main features of the mass and helps to evaluate own goals more consciously.

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Tainturier, François. "The foundation of Mandalay by King Mindon". Thesis, SOAS, University of London, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.599993.

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Carlsson, Emil. "Mining Git Repositories : An introduction to repository mining". Thesis, Linnéuniversitetet, Institutionen för datavetenskap (DV), 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-27742.

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When performing an analysis of the evolution of software quality and software metrics,there is a need to get access to as many versions of the source code as possible. There isa lack of research on how data or source code can be extracted from the source controlmanagement system Git. This thesis explores different possibilities to resolve thisproblem. Lately, there has been a boom in usage of the version control system Git. Githubalone hosts about 6,100,000 projects. Some well known projects and organizations thatuse Git are Linux, WordPress, and Facebook. Even with these figures and clients, thereare very few tools able to perform data extraction from Git repositories. A pre-studyshowed that there is a lack of standardization on how to share mining results, and themethods used to obtain them. There are several tools available for older version control systems, such as concurrentversions system (CVS), but few for Git. The examined repository mining applicationsfor Git are either poorly documented; or were built to be very purpose-specific to theproject for which they were designed. This thesis compiles a list of general issues encountered when using repositorymining as a tool for data gathering. A selection of existing repository mining tools wereevaluated towards a set of prerequisite criteria. The end result of this evaluation is thecreation of a new repository mining tool called Doris. This tool also includes a smallcode metrics analysis library to show how it can be extended.

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Libros sobre el tema "Minion"

Minion. New York, NY: HarperCollins Publishers, 2014.

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The major's minion. Leicester: Ulverscroft, 2003.

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Banks, L. A. Minion: A vampire huntress legend. New York: St. Martin's Paperbacks, 2004.

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Banks, L. A. Minion: A vampire huntress legend. New York: St. Martin's Griffin, 2003.

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Cinco, Paul y Daurio Ken, eds. Despicable me 2 the official minion manual: Your go-to guide for all things Minion! New York: Scholastic, 2013.

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Dominion Marine Insurance Association of River John. Bye-laws of the [Do]minion Marine Insurance Association of River John. [Pictou, N.S.?: s.n., 1987.

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Tamagusuku-son aza Nakandakari Saishi Iinkai. Minton. [Okinawa-ken Shimajiri-gun Tamagusuku-son]: Tamagusuku-son Aza Nakandakari, 1990.

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Jones, Joan. Minton. Princes Risborough, Buckinghamshire: Shire Publications, 1992.

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Despicable Me Minion Made: Mower Minions. Little, Brown Books for Young Readers, 2016.

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Minion. Walden Pond Press, 2014.

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Capítulos de libros sobre el tema "Minion"

Runtuwene, Lucky R., Josef S. B. Tuda, Arthur E. Mongan y Yutaka Suzuki. "On-Site MinION Sequencing". En Single Molecule and Single Cell Sequencing, 143–50. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-6037-4_10.

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Gent, Ian P., Chris Jefferson y Ian Miguel. "Watched Literals for Constraint Propagation in Minion". En Principles and Practice of Constraint Programming - CP 2006, 182–97. Berlin, Heidelberg: Springer Berlin Heidelberg, 2006. http://dx.doi.org/10.1007/11889205_15.

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Abir, Intisar Reza, Iffat Sharmim Shanim y Nova Ahmed. "MINION: A Following Robot Using Ultrasonic Wave". En Advances in Intelligent Systems and Computing, 387–95. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-10-6872-0_36.

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Lang, Kathrin, Vineeth Surendranath, Philipp Quenzel, Gerhard Schöfl, Alexander H. Schmidt y Vinzenz Lange. "Full-Length HLA Class I Genotyping with the MinION Nanopore Sequencer". En Methods in Molecular Biology, 155–62. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8546-3_10.

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Loewenstern, Andrew. "Scheduling the CB1000 Nanoproteomic Analysis System with Python, Tailor, and Minion". En Principles and Practice of Constraint Programming - CP 2009, 65–72. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-642-04244-7_8.

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Karamitros, Timokratis y Gkikas Magiorkinis. "Multiplexed Targeted Sequencing for Oxford Nanopore MinION: A Detailed Library Preparation Procedure". En Methods in Molecular Biology, 43–51. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7514-3_4.

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Mallett, Andrew. "Targeting Salt Minions". En Salt Open, 43–55. Berkeley, CA: Apress, 2021. http://dx.doi.org/10.1007/978-1-4842-7237-4_4.

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Lucchetti, Roberto. "Minima and quasi minima". En CMS Books in Mathematics, 55–77. New York, NY: Springer New York, 2006. http://dx.doi.org/10.1007/0-387-31082-7_4.

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Hangay, George, Susan V. Gruner, F. W. Howard, John L. Capinera, Eugene J. Gerberg, Susan E. Halbert, John B. Heppner et al. "Minima". En Encyclopedia of Entomology, 2401. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6359-6_4631.

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Rácz, Péter. "Minden fordítás. A fordítás minden?" En Četvrt veka beogradske hungarologije, 251–57. Beograd: Filološki fakultet Univerziteta u Beogradu, 2021. http://dx.doi.org/10.18485/25bghun.2021.ch13.

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Actas de conferencias sobre el tema "Minion"

Shrinivas, Gokul. "Minion". En the 24th Annual International Conference. New York, New York, USA: ACM Press, 2018. http://dx.doi.org/10.1145/3241539.3267730.

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Reiss, Steven P. y Qi Xin. "A Framework for a Programmer's Minion". En 2017 IEEE/ACM 39th International Conference on Software Engineering Companion (ICSE-C). IEEE, 2017. http://dx.doi.org/10.1109/icse-c.2017.54.

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Ghasal, Prahalad Sahay, Prakriti Trivedi, Anjali Chandwani, Madan Lal Tetrawal, Abhinash Jha y Ayush Kumar. "Payload minion stenographic technique for color images". En 2014 International Conference on Optimization, Reliabilty, and Information Technology (ICROIT). IEEE, 2014. http://dx.doi.org/10.1109/icroit.2014.6798343.

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Purtilo, J. M. "MINION: an environment to organize mathematical problem solving". En the ACM-SIGSAM 1989 international symposium. New York, New York, USA: ACM Press, 1989. http://dx.doi.org/10.1145/74540.74559.

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Wotawa, Franz, Mihai Nica y Bernhard K. Aichernig. "Generating Distinguishing Tests Using the Minion Constraint Solver". En 2010 IEEE International Conference on Software Testing Verification and Validation Workshop (ICSTW). IEEE, 2010. http://dx.doi.org/10.1109/icstw.2010.11.

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Georgoulas, Christos, Thomas Linner y Thomas Bock. "A novel MiniOn Agent Assisted Robotic Kitchen Platform". En 29th International Symposium on Automation and Robotics in Construction; Held jointly with the 8th World Conference of the International Society for Gerontechnology. International Association for Automation and Robotics in Construction (IAARC), 2012. http://dx.doi.org/10.22260/isarc2012/0077.

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Corral Vicente, Antonio Enrique y Eduardo Moya de la Torre. "Nuevas tecnologías en manipuladores ingrávidos: manipulador ingrávido 4.0 (MINIon 4.0)". En XL Jornadas de Automática. Universidade da Coruña. Servizo de Publicacións, 2020. http://dx.doi.org/10.17979/spudc.9788497497169.389.

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Kchouk, Mehdi y Mourad Elloumi. "Hybrid error correction approach and de novo assembly for minion sequencing long reads". En 2016 IEEE International Conference on Bioinformatics and Biomedicine (BIBM). IEEE, 2016. http://dx.doi.org/10.1109/bibm.2016.7822504.

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Shetty, Akshat, Adhrit Shetty, Kevin Sijo Puthusseri y Radha Shankaramani. "An Improved Ant Colony optimization Algorithm: Minion Ant(MAnt) and its Application on TSP". En 2018 IEEE Symposium Series on Computational Intelligence (SSCI). IEEE, 2018. http://dx.doi.org/10.1109/ssci.2018.8628805.

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Liu, Kan, Shangang Jia, Qian Du y Chi Zhang. "NanoAsPipe: A transcriptome analysis and alternative splicing detection pipeline for MinION long-read RNA-seq". En 2017 IEEE International Conference on Bioinformatics and Biomedicine (BIBM). IEEE, 2017. http://dx.doi.org/10.1109/bibm.2017.8217937.

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Informes sobre el tema "Minion"

Mayes, Robyn, Bree Hurst y Amelia Hine. PREDICT: Principles of Good Mining Checklist. Queensland University of Technology, julio de 2021. http://dx.doi.org/10.5204/rep.eprints.212047.

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CONTEXT: Social Licence to Operate (SLO) encompasses the broad socio-political understanding on the part of multiple stakeholders that a mining operation’s social and environmental impacts and measures are legitimate and acceptable. The multiple and variously interacting stakeholder groups— local communities, environmental actors, Indigenous communities, regulators, local governments, industry peak bodies, financiers, affiliated businesses—have the proven capacity to confer and/or disrupt a mining operation’s SLO. The presence or absence of a SLO can have significant consequences not only for stakeholder groups, including the mining operation, but also for the shared development of a good mining future. Conceptualisation of what is ‘good mining’ is central to future planning and decisions around development, adoption and reception of new technologies and sustainable mining futures. CHECKLIST PURPOSE This first of its kind tool seeks to facilitate genuine multistakeholder interactions and development of a dynamic shared SLO to advance good mining.

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Nolan E. Hertel y Dwayne Blaylock. Minior Actinide Doppler Coefficient Measurement Assessment. Office of Scientific and Technical Information (OSTI), abril de 2008. http://dx.doi.org/10.2172/927795.

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Singhal, R. K., H. Sahay y S. Ghosh. Continuous mining systems for oil sands mining. Natural Resources Canada/ESS/Scientific and Technical Publishing Services, 1986. http://dx.doi.org/10.4095/304974.

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Lee, K., H. Kargupta, B. G. Stafford, K. L. Buescher y B. Ravindran. Data mining. Office of Scientific and Technical Information (OSTI), diciembre de 1998. http://dx.doi.org/10.2172/334314.

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Parsons, M. Metal mining. Natural Resources Canada/ESS/Scientific and Technical Publishing Services, 2016. http://dx.doi.org/10.4095/311153.

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Singhal, R. K. Mining planning and equipment selection in coal mining. Natural Resources Canada/ESS/Scientific and Technical Publishing Services, 1985. http://dx.doi.org/10.4095/304824.

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Singhal, R. K. y T. S. Golosinski. Continuous mining systems for surface mining of coal. Natural Resources Canada/ESS/Scientific and Technical Publishing Services, 1986. http://dx.doi.org/10.4095/304984.

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Leith, W. Adushkin, V. y A. Spivak. Large mining blasts from the Kursk Mining Region, Russia. Office of Scientific and Technical Information (OSTI), enero de 1997. http://dx.doi.org/10.2172/632818.

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Stewart, D. B. Surface mining laboratory. Natural Resources Canada/ESS/Scientific and Technical Publishing Services, 1986. http://dx.doi.org/10.4095/304902.

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Hasegawa, H. S. Mining induced seismicity. Natural Resources Canada/ESS/Scientific and Technical Publishing Services, 1988. http://dx.doi.org/10.4095/122734.

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