Bibliografías temáticas / Mucor circinelloides

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Literatura académica sobre el tema "Mucor circinelloides"

Autor: Grafiati
Publicado: 4 de junio de 2021
Última modificación: 1 de febrero de 2022

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Artículos de revistas sobre el tema "Mucor circinelloides"

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Saito, S., T. J. Michailides y C. L. Xiao. "Mucor Rot—An Emerging Postharvest Disease of Mandarin Fruit Caused by Mucor piriformis and other Mucor spp. in California". Plant Disease 100, n.º 6 (junio de 2016): 1054–63. http://dx.doi.org/10.1094/pdis-10-15-1173-re.

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In recent years, an emerging, undescribed postharvest disease was observed on mandarin fruit after extended storage in California. We collected decayed mandarin fruit from three citrus packinghouses in the Central Valley of California in 2015 and identified this disease as Mucor rot caused by Mucor spp. Mucor rot occurred in 11 of the 15 grower lots sampled, and the percentage of Mucor rot in the total decayed fruit varied among affected grower lots, ranging from 3.3 to 93.1% with an average of 49.2%. In total, 197 isolates of Mucor spp. were obtained from decayed mandarin fruit and identified based on internal transcribed spacer sequence and morphological characteristics. Of the 197 isolates, 182 (92.4%) were identified as Mucor piriformis, 7 (3.6%) were M. circinelloides (6 M. circinelloides f. lusitanicus and 1 M. circinelloides f. circinelloides), 4 (2%) were M. racemosus f. racemosus, 3 (1.5%) were M. hiemalis, and 1 (0.5%) was M. mucedo. All species grew at 0 and 5°C, except M. circinelloides, which did not grow at 0°C. Mycelial growth was arrested at 27°C for M. piriformis; 35°C for M. racemosus f. racemosus, M. circinelloides f. lusitanicus, M. hiemalis and M. mucedo; and 37°C for M. circinelloides f. circinelloides. Optimal mycelial growth occurred at 20°C for M. piriformis and M. mucedo, 25°C for M. racemosus f. racemosus and M. hiemalis, 27°C for M. circinelloides f. lusitanicus, and 30°C for M. circinelloides f. circinelloides. M. piriformis grew significantly faster than the other four species at 5 and 20°C, and M. mucedo was the slowest in growth among the five species. Sporangiospores of M. piriformis, M. racemosus f. racemosus, and M. hiemalis germinated at both 5 and 20°C. M. circinelloides germinated at 20°C but did not germinate at 5°C after incubation for 48 h. All five Mucor spp. caused decay on mandarin fruit inoculated with the fungi, and the lesion size caused by M. piriformis was significantly larger than that caused by other species at both 5 and 20°C. Our results indicated that Mucor rot in mandarin fruit in California is caused by Mucor spp. consisting of M. piriformis, M. circinelloides, M. racemosus f. racemosus, M. hiemalis, and M. mucedo, with M. piriformis being the dominant and most virulent species. Previously, M. racemosus was reported on citrus. This is the first report of Mucor rot in citrus caused by M. piriformis, M. circinelloides, M. hiemalis, and M. mucedo.
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Wagner, L., J. B. Stielow, G. S. de Hoog, K. Bensch, V. U. Schwartze, K. Voigt, A. Alastruey-Izquierdo, O. Kurzai y G. Walther. "A new species concept for the clinically relevant Mucor circinelloides complex". Persoonia - Molecular Phylogeny and Evolution of Fungi 44, n.º 1 (29 de junio de 2020): 67–97. http://dx.doi.org/10.3767/persoonia.2020.44.03.

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Mucor species are common soil fungi but also known as agents of human infections (mucormycosis) and used in food production and biotechnology. Mucor circinelloides is the Mucor species that is most frequently isolated from clinical sources. The taxonomy of Mucor circinelloides and its close relatives (Mucor circinelloides complex – MCC) is still based on morphology and mating behaviour. The aim of the present study was a revised taxonomy of the MCC using a polyphasic approach. Using a set of 100 strains molecular phylogenetic analysis of five markers (ITS, rpb1, tsr1, mcm7, and cfs, introduced here) were performed, combined with phenotypic studies, mating tests and the determination of the maximum growth temperatures. The multi-locus analyses revealed 16 phylogenetic species of which 14 showed distinct phenotypical traits and were recognised as discrete species. Five of these species are introduced as novel taxa: M. amethystinus sp. nov., M. atramentarius sp. nov., M. variicolumellatus sp. nov., M. pseudocircinelloides sp. nov., and M. pseudolusitanicus sp. nov. The former formae of M. circinelloides represent one or two separate species. In the MCC, the simple presence of well-shaped zygospores only indicates a close relation of both strains, but not necessarily conspecificity. Seven species of the MCC have been implemented in human infection: M. circinelloides, M. griseocyanus, M. janssenii, M. lusitanicus, M. ramosissimus, M. variicolumellatus, and M. velutinosus.
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Gonzalez-Hernandez, G. A., L. Herrera-Estrella, V. Rocha-Ramirez, M. I. G. Roncero y J. F. Gutierrez-Corona. "Biolistic transformation of Mucor circinelloides". Mycological Research 101, n.º 8 (agosto de 1997): 953–56. http://dx.doi.org/10.1017/s0953756297003614.

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Somogyv�ri, F., Cs V�gv�lgyi, T. Papp y L. Ferenczy. "Electrofusion of Mucor circinelloides protoplasts". Biotechnology Techniques 10, n.º 8 (agosto de 1996): 607–10. http://dx.doi.org/10.1007/bf00157370.

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Nagy, �., Cs V�gv�lgyi, �. Balla y L. Ferenczy. "Electrophoretic karyotype of Mucor circinelloides". Current Genetics 26, n.º 1 (julio de 1994): 45–48. http://dx.doi.org/10.1007/bf00326303.

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Tan, Yun Nian, Pei Pei Lee y Wei Ning Chen. "Dual Extraction of Crustacean and Fungal Chitosan from a Single Mucor circinelloides Fermentation". Fermentation 6, n.º 2 (12 de abril de 2020): 40. http://dx.doi.org/10.3390/fermentation6020040.

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Mucor circinelloides is a fungus that has been reported to produce ethanol, oil, protein, phosphate and glucosamine, depending on the available nutrients and cultivation conditions. Due to its ability to produce extracellular proteases, it is able to ferment polypeptides and amino acids broken down from various protein sources. In this study, we attempted to culture the Mucor circinelloides on waste substrates to deproteinize prawn shells for the extraction of chitin and subsequently extract chitosan from its fungal cell wall in a concurrent fermentation. The physio-chemical properties of the extracted crustacean chitin and fungal chitosan were determined by Fourier Transform Infrared Spectroscopy (FTIR) and Elemental Analysis (EA). We found that Mucor circinelloides grown on okara and coffee waste behaved as an excellent protease producer and successfully extracted chitin from prawn shells with a degree of deacetylation of 69.94% and 68.82%, respectively, comparable to commercial chitin (70.46%). The fungal chitosan extracted from the fermentation of Mucor circinelloides on red grape pomace substrate showed a degree of deacetylation of 61.05%, comparable to commercial chitosan (64.00%). Our results suggested feasibility of extracting chitosan from seafood waste-streams using cost-effective microbial fermentation.
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Chaparro Pedraza, Aura Patricia y Silvia E. Campuzano F. "Aislamiento, identificación y evaluación de la actividad antimicrobiana de metabolitos producidos por Mucor circinelloides (Cepa Nativa SPG 321)". Nova 16, n.º 29 (10 de septiembre de 2018): 63–70. http://dx.doi.org/10.22490/24629448.2690.

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Objetivo. Identificar y evaluar la capacidad antimicrobiana de los metabolitos aislados a partir delproceso de fermentación en una cepa de Mucor circinelloides. Método. En el presente estudio setrabajó la cepa nativa de Mucor circinelloides SPG 321 suministrada por el Grupo de Investigaciónde Biotrasformación (GIBUJ), con el fin de evaluar su actividad antimicrobiana. La cinética decrecimiento determinó que la ideofase culminó a la hora 264, hora determinada como la finalizaciónde la fermentación. Se separó el micelio del caldo y posteriormente se fraccionaron los extractos concromatografías de capa fina y columna en sílica gel, eluidas con diferentes relaciones de solventes.Resultados. Los resultados arrojados por la técnica de gases acoplado a masas CG-EM confirmanla importancia de Mucor circinelloides en la producción de ácidos grasos insaturados. A partir delmicelio se obtuvo un esterol, compuesto M. cB3. La fracción CHCl3 en biomasa mostró actividadinhibitoria para los microorganismos Gram positivos.
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Hussain, Nazir, Hameed, Yang, Mustafa y Song. "Optimization of Diverse Carbon Sources and Cultivation Conditions for Enhanced Growth and Lipid and Medium-Chain Fatty Acid (MCFA) Production by Mucor circinelloides". Fermentation 5, n.º 2 (23 de abril de 2019): 35. http://dx.doi.org/10.3390/fermentation5020035.

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Leeuw, Ntsoaki J., Chantel W. Swart, Desmond M. Ncango, Wilmarie M. Kriel, Carolina H. Pohl, Pieter W. J. van Wyk y Johan L. F. Kock. "Anti-inflammatory drugs selectively target sporangium development in Mucor". Canadian Journal of Microbiology 55, n.º 12 (diciembre de 2009): 1392–96. http://dx.doi.org/10.1139/w09-096.

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It is known that acetylsalicylic acid, an anti-inflammatory and anti-mitochondrial drug, targets structure development and functions of yeasts depending on elevated levels of mitochondrial activity. Using antibody probes, we previously reported that sporangia of Mucor circinelloides also contain increased mitochondrial activity, yielding high levels of 3-hydroxyoxylipins. This was, however, not found in Mortierella alpina (subgenus Mortierella ). In this study we report that acetylsalicylic acid (aspirin) also targets sporangium development of Mucor circinelloides selectively, while hyphae with lower levels of mitochondrial activity are more resistant. Similar results were obtained when the anti-inflammatory compounds benzoic acid, ibuprofen, indomethacin, and salicylic acid were tested. The anti-inflammatory drugs exerted similar effects on this dimorphic fungus as found under oxygen-limited conditions. Interestingly, sporangium development of Mortierella alpina was found not to be selectively targeted by these drugs. Mortierella alpina, which could not exhibit dimorphic growth under oxygen-limited conditions, was also more sensitive to the anti-inflammatory drugs when compared with Mucor circinelloides. These results prompt further research to assess the applicability of these antimitochondrial antifungals to protect plants and animals against Mucor infections.
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Dickinson, Liliane, Marianne Harboe, Robyn van Heeswijck, Per Strøman y Lars Peter Jepsen. "Expression of active mucor miehei aspartic protease in Mucor circinelloides". Carlsberg Research Communications 52, n.º 4 (julio de 1987): 243–52. http://dx.doi.org/10.1007/bf02907167.

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Tesis sobre el tema "Mucor circinelloides"

1

Jackson, Frances Mary. "Lipid biosynthesis in Mucor circinelloides". Thesis, University of Bristol, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.319098.

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Silva, Cinthya Barbosa da. "Valorização biotecnológica do glicerol residual do biodiesel para produção de biomassa e lipídeo unicelular (sco) por mucor circinelloides ucp 050 isolado de manguezal". Universidade Católica de Pernambuco, 2014. http://www.unicap.br/tede//tde_busca/arquivo.php?codArquivo=1016.

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O glicerol (também conhecido como glicerina) é um subproduto importante no processo de fabricação de biodiesel, representando cerca de 10% do subproduto formado na reação de transesterificação deste biocombustível. O custo de purificação deste subproduto é alto para que o mesmo seja inserido nas indústrias de alimentos, farmacêutica, e cosméticos. Dessa forma, vários métodos de eliminação e utilização deste glicerol residual têm sido tentados, incluindo combustão, compostagem, digestão anaeróbica, ração animal, bem como sua conversão biológica em produtos de valor agregado. Além disso, o estabelecimento de uma dinâmica de inovação tecnológica e desenvolvimento cientifico-tecnológico relacionadas à glicerina como uma possível matéria-prima para os processos biotecnológicos microbianos é de grande relevância. Nesse estudo foram avaliados os efeitos da glicerina residual do biodiesel sobre o crescimento, a morfologia, expressão de enzimas oxidativas; peroxidase, superóxido dismutase e glutation-S-transferase, peroxidação de lipídeos, produção de lipídeos e perfil de ácidos graxos do fungo Mucor circinelloides. Os resultados revelam alterações em todos os parâmetros testados. A presença de glicerina o meio de cultura induz a um aumento de biomassa celular comparado ao crescimento em glicose e glicerol puro. O reaproveitamento do resíduo revela-se uma alternativa viável minimizar o excesso de glicerina produzido para a produção de biomassa, lipídeos e enzimas antioxidantes, apontando o potencial industrial e biotecnológico do isolado. Além disso, da habilidade de utilização do subproduto, o fungo poderia ser utilizado como substrato para a produção de biodiesel.
Glycerol (also known as glycerin) is an important by-product in the process of manufacture of biodiesel, corresponding to 10% of the by-product formed in the reaction of transesterificação of this biofuel. The purification costs of raw glycerol is high and disables its use in the industries of foods, druggs, and cosmetics. Thus, several methods of reuse of residual glycerol have been attemped, including combustion, anaerobic digestion, animal food, as well as its biological conversion in products of aggregate value. Moreover, the establishment of a dynamics of related technological innovation and scientific development to the glycerin as a possible raw material for the microbial biotechnological processes are of great importance. In this study the effects of the residual glycerin of biodiesel were evaluated on the growth, biomass production, morphology, oxidative enzymes: superoxide dismutase; gluthation-S-Transferase and catalase, lipid peroxidation, production of lipids and fatty acid of Mucor circinelloides. The results revealed alterations in all parameters tested. The glycerin presence in culture medium induced increase in cellular growth and biomass compared to growth in glucose and pure glycerol. The residue metabolization is a viable alternative for exceeding glycerin produced for production of biomass, lipids and antioxidant enzymes, pointing the industrial and biotechnological potential application of the isolate. Moreover, the ability of use of the by-product also can be applied as substrate for biodiesel production.
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Li, Yonghua. "A biochemical and molecular study of lipid biosynthesis in Mucor circinelloides". Thesis, University of Hull, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.395508.

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Aidil, bin Abdul Hamid Aidil Bin Abdul. "The biochemistry of lipid accumulation in Mucor circinelloides and Mortierella alpina". Thesis, University of Hull, 1998. http://hydra.hull.ac.uk/resources/hull:8031.

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1. The profile and biochemistry of growth and lipid accumulation in M. circinelloides and Mt. alpina were investigated. 2. A nitrogen-limited condition was vital in triggering lipid accumulation in both fungi, which was in agreement with previous work reported in oleaginous yeasts (Botham and Ratledge, 1979; Boulton and Ratledge, 1984). Good growth and lipid production by M. circinelloides were obtained only when it was grown in fermenters. Growth in stirred bottles (whirlipots) did not result in high lipid yields. This was caused by the anaerobic nature of cultivation in the whirlipots which affected the utilization of ammonium by the cells. As a result the cultures became carbon-limited instead of nitrogen-limited. Conversely, in a fermenter culture which had an efficient aeration, the culture reached a nitrogen-limited condition at an early stage of the incubation which led to a higher lipid production of the cells. 3. The lipid production in both fungi increased in parallel with the increase in the C:N ratio of the medium but the fatty acid compositions were not affected. 4. Ten enzymes potentially linked to the regulation of lipid biosynthesis (fatty acid synthase, acetyl-CoA carboxylase, ATP:Citrate lyase, AMP:deaminase, carnitine acetyl transferase, malic enzyme, glucose-6- phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, NADP:isocitrate dehydrogenase and NAD:isocitrate dehydrogenase) were detected in both fungi. In both fungi, the profile of all enzymes stated above was similar with the activities increased coincidentally with the depletion of ammonia in the medium. 5. The only differences in the enzymatic profiles of the two fungi was the early depletion of ME activity in M. circinelloides where it disappeared after approximately 40 h of incubation, coincident with the cessation of lipid accumulation, although other key enzymes of lipid biosynthesis (FAS, ACC and ACL) together with the activities of other NADPHgenerating enzymes were still active and the glucose was still present. Conversely, ME in Mt. alpina culture was present until the late stage of fermentation and the cell lipid continued to increase until the end of the fermentation. This suggests ME is a major provider of NADPH for lipid biosynthesis which was in agreement with previous observations in Aspergillus nidu/ans (Wynn and Ratledge, 1997) and M. circinelloides (Wynn et al., 1997). 6. The depletion of ME activity in M. circinelloides after approximately 40 h of incubation was as a result of the cessation of the protein from being synthesized, triggered by the depletion of ammonium in the culture. This - was evident as malic enzyme activity returned after its initial depletion when ammonium tartrate was added into the culture. Also, the restitution of malic enzyme activity was prevented when cycloheximide, a protein synthesis inhibitor, was added simultanously with the addition of ammonium tartrate. 7. The NAD:isocitrate dehydrogenase from both fungi showed an increased affinity for its substrate, isocitrate, in, the presence of AMP. However, the enzyme did not show an absolute requirement for AMP for its activity as it could still be activated in the absence of AMP at a saturating concentration of isocitrate. 8. ME was purified some 20-fold purification from both fungi. Both showed a similar Km values for NADP (approximately 0.04 mM) but a slightly higher Km value for malate was obtained in Mt. alpina compared to M. circinelloides (1 mM and 0.4 mM, respectively). 9. ME from both fungi showed various degrees of inhibition by tartronic acid, oxaloacetate, palmitoyl-CoA and oleoyl-CoA. At 10 mM, tartronic acid caused approximately 40 % inhibition in the activity of ME from both fungi while OAA inhibited ME from M. circinelloides more strongly (70 %) than that from Mt. alpina ( 45 % ). At a final concentration of 1 mM, palmitoyl-CoA and oleoyl-CoA caused a 100 % inhibition on ME from M. circinelloides and approximately 90 % on ME from Mt. alpina. 10. FAS purified from both fungi showed a similar Km values for malonyl-CoA and acetyl-CoA (approximately 0.013 and 0.016 mM, - respectively) while a higher Km value for NAOPH was observed in Mt. alpina compared to M. circinelloides (0.038 and 0.01 mM, respectively). 11. Despite a range of experiment using different approaches, no direct evidence of a physical association between FAS and ME was obtained when experiments were performed to observe the formation of complexes between the two enzymes in vitro.
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Trieu, Trung Anh. "Identifying components of the non-Canonical RNA silencing mechanism in Mucor circinelloides = Identificación de componentes del mecanismo no canónico de silenciamiento mediado por RNA en Mucor circinelloides". Doctoral thesis, Universidad de Murcia, 2015. http://hdl.handle.net/10803/308346.

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Introducción: El conocimiento cada vez mayor sobre la relevancia funcional de los pequeños RNAs endógenos (esRNAs) como riboreguladores ha estimulado la identificación y caracterización de estas moléculas en numerosos eucariotas. En el hongo basal Mucor circinelloides, un patógeno oportunista humano, se han descrito esRNAs que regulan la expresión de muchos genes que codifican proteínas. En la biogénesis de estos esRNAs participan una RNasa III denominada Dicer, una proteína Argonauta y dos RNA polimerasas dependientes de RNA (RdRP). Además de participar en esta ruta canónica de silenciamiento mediado por RNA, los genes rdrp de M. circinelloides están implicados en la producción de una nueva clase de esRNA, que son independientes de Dicer. Estos esRNAs muestran un sesgo muy fuerte en la polaridad de las cadenas y una distribución aleatoria de tamaños, lo que sugiere que son productos de degradación de RNAm endógenos. El objetivo global de esta tesis es caracterizar esta posible ruta de degradación de RNAm, identificar la RNasa implicada y utilizar los mecanismos de silenciamiento de Mucor para realizar un análisis funcional a escala genómica. Estos objetivos globales se concretan en los siguientes: Objetivos: i) Análisis de la función de la ruta independiente de Dicer y dependiente de RdRp en la regulación de la expresión génica. ii) Identificación in silico de RNasas de M. circinelloides candidatas a participar en la ruta de degradación independiente de Dicer. iii) Análisis del papel de los genes candidatos en la producción de esRNAs independientes de Dicer y dependientes de RdRP. iv) Construcción de genotecas genómicas para el análisis funcional de genes mediante RNA de interferencia (RNAi), utilizando vectores de silenciamiento para identificar secuencias de M. circinelloides con un posible papel en patogénesis. v) Generación de mutantes nulos de los genes candidatos para confirmar el fenotipo e investigar su papel en la patogénesis de Mucor. Métodos: El análisis de la función génica se llevó a cabo mediante manipulaciones genéticas in vitro e in vivo. Estas incluyen métodos para aislar, amplificar y analizar la expresión de genes específicos y para la transformación genética de células vivas. Se construyeron genotecas de DNA genómico en vectores de silenciamiento con promotores duales para el análisis funcional del genoma y la identificación de genes candidatos con un posible papel en la patogénesis de Mucor. Se utilizaron análisis fenotípicos para evaluar las funciones de los genes candidatos en el crecimiento, la morfogénesis y la virulencia de este hongo. Resultados: Los análisis de expresión demostraron que la nueva ruta independiente de Dicer y dependiente de RdRP regula la expresión génica mediante la degradación específica de RNAm por una RNasa previamente desconocida. Esta ruta regula principalmente genes conservados implicados en metabolismo y procesos de señalización celular, tales como los requeridos para la biosíntesis del grupo hemo, y controla las respuestas a señales ambientales específicas. La búsqueda en el genoma de Mucor identificó varias RNasas candidatas, y el análisis funcional de los mutantes nulos correspondientes permitió identificar una nueva proteína, R3B2, que es específica de hongos basales. Esta RNasa III participa tanto en la ruta no canónica de degradación independiente de Dicer, como en la ruta canónica de silenciamiento dependiente de Dicer, lo que pone de manifiesto su papel crucial en la biogénesis y función de los esRNAs reguladores. Se ha utilizado el mecanismo canónico de silenciamiento para llevar a cabo el análisis funcional a escala genómica en Mucor, para lo cual se construyeron dos genotecas genómicas basadas en RNAi. La introducción de estas genotecas en M. circinelloides permitió identificar varios transformantes con fenotipos anormales. Los análisis moleculares y de silenciamiento demostraron que dos genes específicos fueron los responsables de las alteraciones fenotípicas estudiadas. El análisis fenotípico de los mutantes nulos correspondientes confirmó el papel de dichos genes en la morfogénesis y patogénesis de M. circinelloides. Conclusiones: Se ha identificado y caracterizado una nueva ruta no canónica de silenciamiento, independiente de Dicer y dependiente de RdRP, que regula la expresión génica mediante la degradación de RNAm específicos. La RNasa implicada en esta ruta, denominada R3B2, presenta una arquitectura de dominios única, es específica de hongos basales y también está implicada en el mecanismo canónico de RNAi. Estos resultados asignan un nuevo papel para las proteínas RdRP en un proceso de degradación de RNA que podría representar el primer paso en la evolución del RNAi. Se ha desarrollado con éxito un procedimiento para realizar análisis funcional a gran escala utilizando RNAi, lo que ha permitido identificar dos genes que participan en la morfogénesis de Mucor. El gen mcmyo5 codifica una miosina de clase V que juega un papel esencial en la morfogénesis y la patogénesis de Mucor. El gen mcclasp codifica una proteína CLASP, que también participa en la morfogénesis, pero no juega ningún papel importante en la patogénesis de Mucor.
The increasing knowledge on the functional relevance of endogenous small RNAs (esRNAs) as riboregulators has stimulated the identification and characterization of these molecules in numerous eukaryotes. In the basal fungus Mucor circinelloides, an emerging opportunistic human pathogen, esRNAs that regulate the expression of many protein coding genes have been described. These esRNAs share common machinery for their biogenesis consisting of an RNase III endonuclease Dicer, a single Argonaute protein and two RNA-dependent RNA polymerases. Besides participating in this canonical dicer-dependent RNA interference (RNAi) pathway, the M. circinelloides rdrp genes are involved in the production of a novel dicer-independent esRNA class, which showed a very strong strand bias, being exclusively sense to the mRNAs, and a random spread of size distribution, suggesting that they are degradation products of endogenous mRNAs. The overall objective of this thesis is to characterize this putative mRNA degradation pathway, identify the RNase involved and use the Mucor silencing mechanisms for whole-genome functional analysis. These global objectives are specified in the following: Objectives: i) Functional analysis of the rdrp-dependent dicer-independent pathway in the regulation of gene expression. ii) In silico identification of M. circinelloides candidate RNases to be involved in the dicer-independent degradation pathway. iii) Functional studies of the candidate genes in the production of rdrp-dependent dicer-independent esRNAs. iv) Construction of genomic libraries for functional analysis by knocking-down genes using silencing vectors to identify M. circinelloides sequences with putative roles in pathogenesis. v) Generation of null mutants for each candidate gene to confirm the phenotype and investigate their roles in Mucor pathogenesis. Methods: in vivo and in vitro genetic manipulations were used to analyze gene functions. These include methods to isolate, amplify and analyze the expression of specific genes and genetic transformation of living cells. Genomic DNA libraries were constructed in silencing vectors containing dual promoters for whole-genome functional analysis and identification of candidate genes with a possible role in Mucor pathogenesis. Phenotypic analyses were used to evaluate the functions of candidate genes in growth, morphogenesis and virulence of this fungus. Results: Expression analysis demonstrated that the new rdrp-dependent dicer-independent pathway regulates gene expression by promoting the specific degradation of mRNAs by a previously unknown RNase. This pathway mainly regulates conserved genes involved in metabolism and cellular processes and signaling, such as those required for heme biosynthesis, and controls responses to specific environmental signals. Searching the Mucor genome for candidate RNases to participate in this pathway, and functional analysis of the corresponding knockout mutants identified a new protein, R3B2, which is only found in basal fungi. This RNase III-like protein participates in both the rdrp-dependent dicer-independent non-canonical pathway and the canonical dicer-dependent RNAi pathway, highlighting its crucial role in the biogenesis and function of regulatory esRNAs. RNAi was applied to carry out whole-genome functional analysis in Mucor. Two RNAi-based genomic libraries were constructed. Introduction of these libraries into M. circinelloides identified several transformants with abnormal phenotypes. Silencing and molecular analyses demonstrated that two specific genes were responsible for the phenotypic alterations. Phenotypic analyses of the corresponding disruption mutants revealed the role of those genes in M. circinelloides morphogenesis and pathogenesis. Conclusions: A novel non-canonical RNA silencing mechanism promoting mRNA degradation in M. circinelloides has been identified and characterized. This pathway is rdrp-dependent dicer-independent and regulates gene expression by degrading specific mRNAs. The RNase involved in this pathway, R3B2, presents unique domain architecture and it is also involved in the canonical dicer-dependent RNAi pathway. Our results expand the role of RdRPs in gene silencing and reveal the involvement of these proteins in a new RNA degradation process that could represent the first step in the evolution of RNAi. A new approach for large-scale functional genomics using RNAi has been successful developed in M. circinelloides. Two genes that participate in Mucor morphogenesis have been identified. Gene mcmyo5 encodes a Myosin class V protein that plays an essential role in Mucor morphogenesis and pathogenesis. Gene mcclasp encodes a CLASP protein, which is also involved in morphogenesis, but does not play any significant role in Mucor pathogenesis.
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Silva, Franco Fátima. "Regulación de las respuestas a la luz en el hongo mucor circinelloides". Doctoral thesis, Universidad de Murcia, 2013. http://hdl.handle.net/10803/124096.

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Introducción La luz regula gran cantidad de procesos fisiológicos y del desarrollo en muchos organismos. La mayoría de respuestas a la luz caracterizadas en hongos dependen de fotorreceptores de luz azul similares a la proteína Wc-1 de Neurospora crassa. La respuesta a la luz mejor conocida en el hongo Mucor circinelloides es la síntesis de β-caroteno. En este organismo, CrgA, una proteína que muestra características de ligasas de ubiquitina, reprime la carotenogénesis en la oscuridad, afectando también a otros procesos celulares como la esporulación y el crecimiento vegetativo. Objetivos El objetivo principal de la tesis era investigar las vías de transducción de la señal luminosa en hongos, concretamente en las respuestas fototrópica y fotocarotenogénica, utilizando M. circinelloides como modelo. Este objetivo general se estructuró en los siguientes objetivos específicos: 1. Identificación y aislamiento de los genes wc-1 de M. circinelloides. 2. Caracterización de las respuestas fototrópica y fotocarotenogénica. 3. Generación de mutantes nulos para cada gen wc-1 de M. circinelloides y análisis de sus fenotipos. 4. Generación de mutantes dobles para el gen crgA y cada uno de los genes wc-1 y análisis de sus fenotipos. Metodología M. circinelloides presenta el mayor repertorio de herramientas moleculares dentro de los cigomicetos y una respuesta fotocarotenogénica medianamente caracterizada, con mutantes disponibles en genes estructurales y reguladores de la síntesis de carotenos. Los genes mcwc-1 se identificaron y clonaron utilizando una genoteca de M. circinelloides en el fago λ y mediante hibridaciones tipo Southern. Para la caracterización de los genes mcwc-1a, mcwc-1b y mcwc-1c y el estudio de su relación con el gen crgA se generaron mutantes simples y dobles (crgAΔ) nulos para cada uno de los genes mcwc-1. Se analizó el contenido en β-caroteno de sus micelios y el fototropismo de los esporangióforos. También se estudiaron los cambios en los niveles de mRNAs en respuesta a la luz de los genes carotenogénicos y de los genes mcwc-1 utilizando hibridaciones tipo Northern. Además, en el caso de la proteína Mcwc-1b, se analizó su abundancia, sus modificaciones post-traduccionales y su posible interacción con CrgA por medio de hibridaciones tipo Western y co-inmunoprecipitación. Resultados y Conclusiones En primer lugar, utilizando diferentes longitudes de onda del espectro visible, se demostró que los esporangióforos de M. circinelloides poseen fototropismo positivo inducido por luz azul y verde, mientras que la carotenogénesis se induce sólo por luz azul. Además, se identificaron tres genes white-collar-1 en M. circinelloides (mcwc-1a, mcwc-1b y mcwc-1c) que codifican proteínas similares a Wc-1 de Neurospora crassa. Las tres contienen un dominio LOV (luz, oxígeno y voltaje) parecido al presente en receptores de luz azul de plantas y hongos. Los resultados obtenidos en el análisis de los mutantes simples mcwc-1Δ implicaron al gen mcwc-1a en el control del fototropismo y al gen mcwc-1c en la fotocarotenogénesis. Los resultados indican que mcwc-1a y mcwc-1c controlan diferentes rutas de transducción de la luz, aunque es posible que existan interacciones entre ambas rutas, ya que mcwc-1a está implicado en el regulación por luz de la expresión de mcwc-1c. Análisis de los dobles mutantes crgAΔ mcwc-1Δ demostraron que el efecto de crgA en la carotenogénesis depende de mcwc-1b, que actúa como un activador de la carotenogénesis. Por último, se demostró que CrgA está implicado en la mono- y diubiquitilación no degradativa de Mcwc-1b, resultando en su inactivación. La existencia de tres genes mcwc-1 y los fenotipos observados de sus mutantes apoyan la sucesiva duplicación de los genes tipo wc-1 postulada en cigomicetos, seguida de una especialización de sus funciones permitida por la aparición de las nuevas copias.
Introduction Light regulates many developmental and physiological processes in a large number of organisms. Most light responses studied in fungi require blue-light photoreceptors similar to Wc-1 of Neurospora crassa. The best-known light response in the fungus Mucor circinelloides is the biosynthesis of β-carotene. In this organism, CrgA, a protein that shows characteristics of ubiquitin ligases, represses carotenogenesis in the dark and affects also other cellular processes, like sporulation or vegetative growth. Objectives The main goal of this thesis was to study light transduction pathways in fungi, specifically photocarotenogenesis and phototropism responses, using M. circinelloides as a model. The main objective was divided in the following specific objectives: 1. Identification and isolation of wc-1 genes in M. circinelloides. 2. Characterization of photocarotenogenesis and phototropic responses. 3. Generation of knockout mutants of each wc-1 gene in M. circinelloides and analysis of their phenotypes. 4. Generation of double knockout mutants of crgA and each wc-1 gene and analysis of their phenotypes. Methods The most complete molecular toolset in zygomycetes is available in M. circinelloides. The photocarotenogenesis response has been studied in this organism and there are several mutants available in structural and regulatory genes of the biosynthesis of carotenes. A bacteriophage λ genomic library of M. circinelloides and Southern blots were used to identify and clone the three wc-1 genes of M. circinelloides (mcwc-1). To characterize mcwc-1a, mcwc-1b and mcwc-1c and to study their relationships with crgA, simple and double (crgAΔ) knockout mutants for each mcwc-1 gene were generated. Phototropism of sporangiophores and β-carotene content in the mycelium were analysed. Changes in the level of mRNAs in response to light of carotenogenic genes and of mcwc-1 genes were also studied by Northern blots. In addition, the expression levels of Mcwc-1b, its post-translational modifications and it possible interaction with CrgA were analysed using Western blots and co-immunoprecipitation. Conclusions Here, we show that M. circinelloides sporangiophores exhibit a positive phototropism. Analysis of light responses to different light wavelengths within the visible spectrum demonstrated that phototropism is induced by green and blue light, whereas carotenogenesis is only induced by blue light. Three white-collar-1 genes (mcwc-1a, mcwc-1b and mcwc-1c), coding for proteins showing similarity with the Wc-1, were identified in this thesis. All three contain a LOV (light, oxygen or voltage) domain, similar to the one present in fungal and plant blue-light receptors. The study of the knockout mutants for each mcwc-1 gene, showed that mcwc-1c is involved in the light transduction pathway that controls carotenogenesis and that positive phototropism is controlled by mcwc-1a gene. It seems therefore that mcwc-1a and mcwc-1c genes control different light transduction pathways, although cross-talk between both pathways probably exists, because mcwc-1a is involved in the light regulation of mcwc-1c expression. Analysis of double knockout mutants crgAΔ mcwc-1Δ showed that the effect of crgA on carotenogenesis is mediated by mcwc-1b, which acts as a carotenogenesis activator. Finally, it was demonstrated that CrgA is involved in the proteolysis-independent mono- and di-ubiquitylation of Mcwc-1b, which results in its inactivation. The existence and characteristics of the three mcwc-1 genes and the phenotypes of their knockout mutants support the successive duplication of the wc-1 like genes hypothesized in zygomycetes, followed by the functional specialization allowed by the presence of several copies.
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Marques, Nathália Sa Alencar do Amaral. "Produção, caracterização e aplicação de biossurfactante produzido por Mucor circinelloides UCP/WFCC 0001". Universidade Católica de Pernambuco, 2017. http://www.unicap.br/tede//tde_busca/arquivo.php?codArquivo=1297.

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Biossurfactantes são metabólitos secundários de origem microbiana com propriedades tensoativas capazes de atuar como solubilizantes, dispersantes, formadores de espuma e agentes molhantes. Surfactantes de origem biológica são mais vantajosos do que os sintéticos, visto que exibem pouca toxicidade, biodegradabilidade e alta eficiência. Os surfactantes de origem biológica podem ser classificadas de acordo com alguns critérios como, por exemplo, seu peso molecular, carga iônica e tipo de secreção. Contudo, o princípio fundamental de classificação continua a ser a sua estrutura química, o que lhes permite ser divididos nas seguintes classes: glicolipídicos, lipopeptídicos, surfactantes particulares, ácidos graxos, fosfolipídios, lipídios neutros e biossurfactantes poliméricos. Essas biomoléculas possuem uma vasta gama de aplicações na indústria de alimentos, na agricultura, em couro, em cosméticos, na área têxtil, recuperação avançada do petróleo e na biorremediação. A contaminação de solos e águas subterrâneas por hidrocarbonetos tornou-se um foco de grande preocupação, a principal fonte destes poluentes provém de operações rotineiras de lavagem de navios, vazamentos de óleo no mar e, especialmente acidentes relacionados a exploração e transporte de petróleo. O uso de biossurfactante tornou-se uma ferramenta essencial na biorremediação de áreas impactadas por petróleo e seus derivados, estes hidrocarbonetos tornam-se mais acessíveis para degradação microbiana local quando tratados com agentes tensoativos. O biossurfactante lipopeptídico produzido por M. circinelloides apresentou atividade superficial de 26 mN/m relativamente estável em condições ambientais adversas, com uma CMC de 1,5% e rendimento de 6 g/L. O tensioativo exibiu semelhança aos surfactantes químicos por apresentar uma área de deslocamento de óleo de 50 cm2 em dispersão de água-óleo, bem como reduzir a viscosidade do óleo de 843,6 cP para 14,7 cP. Além disso, o biossurfactante foi capaz de recuperar 95,9% de óleo de motor adsorvido em uma amostra de solo argiloso, apresentando considerável potencial para uso em processos de biorremediação, especialmente na indústria de petróleo.
Biosurfactants are secondary metabolites of microbial origin with tensoactive properties capable of acting as solubilizers, dispersants, foaming agents and wetting agents. Surfactants of biological origin are more advantageous than synthetic ones, since they exhibit little toxicity, biodegradability and high efficiency. Surfactants of biological origin can be classified according to some criteria such as, for example, their molecular weight, ionic charge and type of secretion. However, the fundamental principle of classification remains their chemical structure, which allows them to be divided into the following classes: glycolipids, lipopeptides, particular surfactants, fatty acids, phospholipids, neutral lipids and polymeric biosurfactants. These biomolecules have a wide range of applications in the food industry, agriculture, leather, cosmetics, textiles, advanced oil recovery and bioremediation. Contamination of soils and groundwater by hydrocarbons has become a focus of major concern, most in developing countries, because of their wide environmental distribution, which can reach the ground, groundwater and air. Consequently, these toxic compounds are the leading causes of death from toxicity. However, the main source of hydrocarbons in the environment comes from routine shipwreck operations, oil leaks in the seabed and especially accidents related to the exploration and transportation of oil. The use of biosurfactant has become an essential tool in the bioremediation of areas impacted by oil and its derivatives, these hydrocarbons become more compatible for microbial degradation when treated with surfactants. The lipopeptide biosurfactant produced by M. circinelloides showed a relatively stable surface activity of 26 mN / m in adverse environmental conditions, with a CMC of 1.5% and yield of 6 g / L. The surfactant exhibited similarity to chemical surfactants by having a 50 cm2 oil displacement area in water-oil dispersion, as well as reducing the oil viscosity from 843.6 cP to 14.7 cP. The biosurfactant was able to recover 95.9% of adsorbed motor oil in a clayey soil sample, presenting considerable potential for use in bioremediation processes, especially in the petroleum industry.
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Song, Yuanda. "Studies on the role of malic enzyme in lipid accumulation in Mucor circinelloides". Thesis, University of Hull, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342864.

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Sindle, Astrid Elizabeth. "Evaluation of the effect of morphological control of dimorphic Mucor circinelloides on heterologous enzyme production". Thesis, Link to the online version, 2006. http://hdl.handle.net/10019/1207.

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Almeida, Alex Fernando de [UNESP]. "Cultivo de Mucor circinelloides em substratos líquido e sólido para produção de ácidos graxos insaturados". Universidade Estadual Paulista (UNESP), 2007. http://hdl.handle.net/11449/94981.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Os ácidos graxos insaturados, presentes naturalmente em óleos vegetais e peixes de áua fria, possuem potentes atividades biológicas como precursores antiinflamatórios e hormonais. A busca por novas fontes de organismos ricos em ácidos graxos insaturados da família ômega 6 tem-se intensificado. Os fungos filamentosos do solo da ordem Mucorales são conhecidos por possuírem naturalmente altas concentrações destes ácidos graxos. Neste estudo foram inicialmente utilizadas duas linhagens de Mucor circinelloides isoladas de solo de área de caatinga (M1) e cerrado (M2), com as quais foram realizados teste preliminares para selecionar a melhor linhagem produtora de ácido gama-linolênico (AGL). A partir dos resultados obtidos nos cultivos em sistema automatizado foram selecionadas as melhores fontes de carbono, glicose e maltose, para produção de biomassa e também o melhor pH 5,8 para o cultivo submerso. Nos cultivos submerso objetivando-se melhor produção de AGL, selecionouse a linhagem M1 sob as condições de cultivo com pH 5,8, temperatura 25° C, rotação de 150 rpm e inóculo com de 1.107 esporos.mL-1. Os cultivos em substrato sólido foram realizados com farinha de soja, farelo de trigo, polpa cítrica, bagaço de cana-de-açúcar e casca de mandioca, acrescidos com extrato de levedura 1% e mantidos a 25° C, por 168 horas. Foram observados decréscimos no teor de lipídios totais (LT) em todos os cultivos realizados. A farinha de soja foi o melhor substrato para produção de AGL. Este substrato suplementado com diferentes suplementos carbônicos demonstrou que a mistura de óleo de canola: óleo de gergelim 2% (1:1 p/p) foi o maior indutor de AGL (6,2 g.Kg-1 Bioproduto; 26,0 g.g-1 LT). A adição de casca de arroz ao substrato melhorou a aera o do substrato e o aproveitamento deste pelo fungo, acentuando a forma o de AGL (8,4 g.Kg-1 Bioproduto; 36,3 g.g-1 LT). Os testes de temperatura
Unsaturated fatty acid naturally occur in vegetable oils and cold-water fishes. They have powerful biological activities being anti-inflammatory and hormone precursor. The search for new organisms which are rich in omega 6 unsaturated fatty acid family has been intensified. Soil filamentous fungi from Mucorales order are known by having high fatty acids concentrations. Firstly, two Mucor circinelloides strains isolated from caatinga soil area, M1, and from cerrado, M2, have been used. The preliminary tests have been carried out to select the best strain for production of gamma-linolenic acid (GLA). From the automatized incubator system results, either the best carbon sources, glucose and maltose or the best pH, 5.8, have been selected for biomass production in submerged culture. In that cultures focused on better GLA production, M1 strain has been selected under pH 5.8, temperature of 25 C, 150 rpm rotation and with a 1.107 mL-1 spore inocula. The cultures in solid substrate have been carried out with soy flour, wheat bran, citric pulp, sugar cane bagasse and cassava rind, added with 1% yeast extract, at 25 C, for 168 hour. The increase of total lipids (TL) values of all cultures has been observed. The soy flour was the best substrate for GLA production. The substrate added with different carbonic supplements, demonstrated that canola:sesame 2% (1:1 w/w) oil mixture was the best inducer of GLA production (6.2 g.Kg-1 Bioproduct; 26.0 mg.g-1 TL). The rice rind addition in the substrate improved the aeration and the exploitation of it, for the fungi strain, increasing the GLA synthesis (8.4 g.Kg-1 Bioproduct; 36.3 mg.g-1 TL). Temperature tests have shown that 20 C during 144 hours followed by 12 C during 24 hours has leaded to a greater GLA production (12.9 g.Kg-1 Bioproduct; 43.4 mg.g-1 TL) When the spore concentration as increased to 1.108 spores.mL-1 there was an increase in the GLA production (14.2 g.Kg-1; 58.1 mg.g- 1 TL). The culture ...
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Capítulos de libros sobre el tema "Mucor circinelloides"

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Garre, Victoriano, José Luis Barredo y Enrique A. Iturriaga. "Transformation of Mucor circinelloides f. lusitanicus Protoplasts". En Fungal Biology, 49–59. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-10142-2_4.

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Rodríguez-Sáiz, Marta, Juan-Luis de la Fuente y José-Luis Barredo. "Metabolic Engineering of Mucor circinelloides for Zeaxanthin Production". En Microbial Carotenoids From Fungi, 133–51. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-918-1_8.

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Torres-Martínez, Santiago, Rosa M. Ruiz-Vázquez, Victoriano Garre, Sergio López-García, Eusebio Navarro y Ana Vila. "Molecular Tools for Carotenogenesis Analysis in the Zygomycete Mucor circinelloides". En Microbial Carotenoids From Fungi, 85–107. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-918-1_5.

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Nicolás, Francisco E., María Isabel Navarro-Mendoza, Carlos Pérez-Arques, Sergio López-García, Eusebio Navarro, Santiago Torres-Martínez y Victoriano Garre. "Molecular Tools for Carotenogenesis Analysis in the Mucoral Mucor circinelloides". En Methods in Molecular Biology, 221–37. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8742-9_13.

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Iturriaga, Enrique A., Tamás Papp, María Isabel Álvarez y Arturo P. Eslava. "Gene Fusions for the Directed Modification of the Carotenoid Biosynthesis Pathway in Mucor circinelloides". En Microbial Carotenoids From Fungi, 109–22. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-918-1_6.

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Iturriaga, Enrique A., María Isabel Alvarez, Arturo P. Eslava y Tamás Papp. "Expression Vectors and Gene Fusions for the Directed Modification of the Carotenoid Biosynthesis Pathway in Mucor circinelloides". En Methods in Molecular Biology, 239–56. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8742-9_14.

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Papp, Tamás, Árpád Csernetics, Ildikó Nyilasi, Csaba Vágvölgyi y Enrique A. Iturriaga. "Integration of a Bacterial β-Carotene Ketolase Gene into the Mucor circinelloides Genome by the Agrobacterium tumefaciens-Mediated Transformation Method". En Microbial Carotenoids From Fungi, 123–32. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-918-1_7.

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Ruiz-Vázquez, Rosa M. "RNA silencing in filamentous fungi: Mucor circinelloides as a model organism". En RNA Interference Technology, 270–79. Cambridge University Press, 2005. http://dx.doi.org/10.1017/cbo9780511546402.023.

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Antczak, T., D. Hiler, A. Krystynowicz, M. Szczęsna, S. Bielecki y E. Galas. "Activity of immobilised in situ intracellular lipases from Mucor circinelloides and Mucor racemosus in the synthesis of sucrose esters". En Progress in Biotechnology, 221–27. Elsevier, 2000. http://dx.doi.org/10.1016/s0921-0423(00)80071-4.

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Ruiz-Vázquez, Rosa M., Francisco E. Nicolás, Santiago Torres-Martínez y Victoriano Garre. "Distinct RNAi Pathways in the Regulation of Physiology and Development in the Fungus Mucor circinelloides". En Advances in Genetics, 55–102. Elsevier, 2015. http://dx.doi.org/10.1016/bs.adgen.2015.07.002.

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Actas de conferencias sobre el tema "Mucor circinelloides"

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Klanchui, Amornpan, Wanwipa Vongsangnak, Kobkul Laoteng y Asawin Meechai. "In Silico Analysis of Mucor Circinelloides Genome-Scale Model for Enhancing Lipid Production". En CSBio '16: 7th International Conference on Computational Systems-Biology and Bioinformatics. New York, NY, USA: ACM, 2016. http://dx.doi.org/10.1145/3029375.3029383.

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Sá Alencar do Amaral Marques, Nathália, Rafael de Souza Mendonça, Daylin Rubio Ribeaux, Antônio Vinicius Pinho Sá y Galba Maria Campos Takaki. "PRODUÇÃO SUSTENTÁVEL DE BIOSSURFACTANTE POR MUCOR CIRCINELLOIDES UCP 0069 UTILIZANDO RESÍDUOS AGROINDUSTRIAIS COMO SUBSTRATOS". En XVII Encontro Nacional de Estudantes de Engenharia Ambiental e V Fórum Latino-Americano de Engenharia e Sustentabilidade. Recife, Brasil: Even3, 2019. http://dx.doi.org/10.29327/15304.17-160612.

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CARVALHO, A. K. F., J. D. RIVALDI, P. C. M. DA RÓS y H. F. de CASTRO. "TRANSESTERIFICAÇÃO ENZIMÁTICA DE SINGLE CELL OIL (SCO) DE Mucor circinelloides PARA A PRODUÇÃO DE BIODIESEL". En XX Congresso Brasileiro de Engenharia Química. São Paulo: Editora Edgard Blücher, 2015. http://dx.doi.org/10.5151/chemeng-cobeq2014-1536-18779-151151.

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MACIEL, T. C., L. M. N. PAIXÃO, S. L. R. OLIVEIRA, S. O. SANCHO y S. RODRIGUES. "ESTUDO DA INFLUÊNCIA DO pH E DA TEMPERATURA NA PRODUÇÃO DE CELULASES POR Mucor circinelloides". En XX Congresso Brasileiro de Engenharia Química. São Paulo: Editora Edgard Blücher, 2015. http://dx.doi.org/10.5151/chemeng-cobeq2014-0712-24323-149969.

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Acioly, L. M. L., A. A. Barbosa da Silveira, M. N. Anjos, G. K. B. Silva, M. V. Leite, K. Okada y G. M. Campos-Takaki. "Biosurfactant production by Mucor circinelloides using apple peel, vegetable oil and corn steep liquor as substrate". En MICROBES IN APPLIED RESEARCH - Current Advances and Challenges. WORLD SCIENTIFIC, 2012. http://dx.doi.org/10.1142/9789814405041_0069.

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Mai, Lê Ngọc, Nguyễn Thu Hà, Nguyễn Văn Hân, Nguyễn Duy Khánh y Triệu Anh Trung. "TẠO PLASMID RNAi TƯƠNG ỨNG VỚI GEN m2l1 (ID 149958) THUỘC HỌ GEN MYOSIN II Ở NẤM Mucor circinelloides". En HỘI NGHỊ KHOA HỌC QUỐC GIA VỀ NGHIÊN CỨU VÀ GIẢNG DẠY SINH HỌC Ở VIỆT NAM. Nhà xuất bản Khoa học tự nhiên và Công nghệ, 2020. http://dx.doi.org/10.15625/vap.2020.000111.

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ANDRADE, G. S. S., A. K. F. CARVALHO y H. F. DE CASTRO. "ESTABILIDADE OPERACIONAL DA LIPASE DE Mucor circinelloides IMOBILIZADA EM POLIURETANO NA ETANÓLISE DO ÓLEO DE BABAÇU EM REATOR DE LEITO FIXO". En XX Congresso Brasileiro de Engenharia Química. São Paulo: Editora Edgard Blücher, 2015. http://dx.doi.org/10.5151/chemeng-cobeq2014-1774-17627-146405.

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Halim, Yuniwaty, Hardoko, Nicholas Candra y Ratna Handayani. "Optimum Condition for the Production of N-acetylglucosamine from Tiger Shrimp Shells using Semi Pure Extracellular Chitinase Enzyme Produced by Mucor circinelloides". En ASEAN Food Conference. SCITEPRESS - Science and Technology Publications, 2019. http://dx.doi.org/10.5220/0009980501850191.

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RICO, A. L. L., H. F. DE CASTRO y P. C. OLIVEIRA. "PRODUÇÃO ENZIMÁTICA DE BIODIESEL ETÍLICO EM REATOR DE LEITO FIXO E FLUXO CONTÍNUO UTILIZANDO CÉLULAS INTEGRAS DE Mucor Circinelloides IMOBILIZADAS EM ESPUMA DE POLIURETANO". En XX Congresso Brasileiro de Engenharia Química. São Paulo: Editora Edgard Blücher, 2015. http://dx.doi.org/10.5151/chemeng-cobeq2014-1730-17852-151319.

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Lima Pache de Faria, Emanuelle, Daniela Vieira Cortez, Pedro Carlos Oliveira y Heizir Ferreira de Castro. "Influência do tempo espacial na produção de monoésteres de etila por células íntegras de Mucor circinelloides em reator de leito fixo a partir da etanólise de óleo de coco". En Simpósio Nacional de Bioprocessos e Simpósio de Hidrólise Enzimática de Biomassa. Campinas - SP, Brazil: Galoá, 2015. http://dx.doi.org/10.17648/sinaferm-2015-33476.

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