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Tesis sobre el tema "Production de mutants de trypanosomes"

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Publicado: 25 de enero de 2025
Última modificación: 31 de julio de 2025

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1

El, Kadri Mohammad. "Role(s) of glycerol metabolism in the biology of African trypanosomes." Electronic Thesis or Diss., Bordeaux, 2024. http://www.theses.fr/2024BORD0456.

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Trypanosoma brucei, un parasite extracellulaire responsable de la trypanosomiase africaine, doit s’adapter à différents environnements dans ses hôtes mammifères et l’insecte vecteur (la mouche tsétsé). Dans le sang des mammifères, le glucose est la principale source de carbone soutenant une croissance rapide du parasite, en alimentant son métabolisme et la production d'ATP. Lorsque les formes sanguines prolifératives « slender » atteignent des densités cellulaires élevées, un quorum sensing induit leur différenciation en formes non réplicatives « stumpy » (stumpy-QS). Ces formes stumpy-QS prot
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2

Fitzek, Elisabeth. "Production and characterization of mutants of UDP-glucose pyrophosphorylase." Thesis, Umeå University, Plant Physiology, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-26081.

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<p>UDP-glucose pyrophosphorylase (UGPase) is a key component of carbohydrate production in plants, especially with respect to sucrose synthesis/ metabolism, by producing UDP-glucose, a key precursor to sucrose and to many polysaccharides in cell walls. UDP-glucose is also utilized in the synthesis of carbohydrate moiety of glycolipids, glycoproteins and a variety of secondary metabolites, among other functions. The UGPase enzyme may have a rate-limiting function in sugar biosynthesis, and its activity is now known to increase upon variety of abiotic stresses, with possible effects on an overal
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3

Niazy, Abdurahman. "Virulence Factor Production in PyrE Mutants of Pseudomonas Aeruginosa." Thesis, University of North Texas, 2010. https://digital.library.unt.edu/ark:/67531/metadc28458/.

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It has been shown previously in our lab that mutations in the pyrimidine pathway reduced the ability of Pseudomonas aeruginosa to produce virulence factors. Knockout mutations in pyrB, pyrC and pyrD genes of the pyrimidine pathway showed that virulence factor production was decreased. Pyoverdin, pyocyanin, hemolysin, iron chelation, motility, and adherence are all considered virulence factors. Here I further investigate the effects of mutations in the pyrimidine pathway by studying a pyrE mutant. I studied the effect of the pyrE mutation on the production of the above virulence factors. Just l
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4

Kuriakose, Shiby. "Molecular regulation of Trypanosoma congolense-induced proinflammatory cytokine production in macrophages and its modulation by diminazene aceturate (Berenil)." PLOS, Frontiers in Immunology, Elsevier, Sage, 2016. http://hdl.handle.net/1993/31677.

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African trypanosomiasis remains a major health problem to both humans and animals due to lack of effective treatment or vaccine to control the disease. Animal trypanosomiasis is considered one of the most important diseases affecting livestock production and agricultural development in sub-Saharan Africa. Although the use of trypanocides remain the most important method for controlling the disease in animals, the mechanisms of action of these compounds are not completely known. The overall aim of this thesis is to decipher the molecular mechanisms involved in Trypanosoma congolense-induced cyt
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5

Zouheiry, Hassane. "La pénicilline amidase d'Escherichia coli : recherche des meilleurs conditions de production par bactéries recombinées, mise au point de production et de caractérisation de mutants, étude de ces mutants." Nancy 1, 1991. http://www.theses.fr/1991NAN10021.

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Ce travail, réalisé en collaboration avec Rhône-Poulenc santé, a consisté d'une part, à étudier la régulation de l'expression du gène cloné de la pénicilline amidase (PA) dans une souche mutante, d'autre part, à développer une technique de mutagenèse chimique adaptée à l'étude d'enzyme, à l'appliquer au gène PA et à caractériser les mutants obtenus. La souche transformée présente les caractéristiques suivantes par rapport à la souche non transformée: une exigence plus importante en source de carbone, un effet non pas négatif mais positif du glucose sur la production de PA, une expression en pa
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6

Zeyniyev, Araz. "Cephamycin C Production By Streptomyces Clavuligerus Mutants Impaired In Regulation Of Aspartokinase." Master's thesis, METU, 2006. http://etd.lib.metu.edu.tr/upload/12607641/index.pdf.

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Aspartokinase is the first enzyme of the aspartate family amino acids biosynthetic pathway. Cephamycin C is a &amp<br>#946<br>-lactam antibiotic produced as a secondary metabolite via the enzymatic reactions in the lysine branch of this pathway in Streptomyces clavuligerus. The aspartokinase activity of S. clavuligerus is under concerted feedback inhibition by two of the end product amino acids, lysine plus threonine. It is also known that carbon flow through the lysine branch of the aspartate pathway is rate limiting step in the formation of cephamycin C. Therefore, genetic alterations in th
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7

Liu, Xiaoguang. "Production of butyric acid and hydrogen by metabolically engineered mutants of Clostridium tyrobutyricum." Connect to resource, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1124114266.

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Thesis (Ph. D.)--Ohio State University, 2005.<br>Title from first page of PDF file. Document formatted into pages; contains xv, 220 p.; also includes graphics (some col.). Includes bibliographical references (p. 189-201). Available online via OhioLINK's ETD Center
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8

Wahab, Adbul. "Regulation of antibiotic production in Streptomyces coelicolor : phenotypic and transcriptomic analysis of AbsA mutants." Thesis, University of Surrey, 2007. http://epubs.surrey.ac.uk/843422/.

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The AbsA two-component system is a negative regulator of antibiotic biosynthesis in Streptomyces coelicolor. To gain further insight into the role of the AbsA system different in-frame deletion mutations were made in the absA1A2 operon in S. coelicolor MT1110, MT1110DeltacdaR and S. lividans 1326. Phenotypic analysis of DeltaabsA1A2 and DeltaabsA2 in the MT1110 derivatives showed that CDA was produced early on Oxoid nutrient agar relative to the parent strain. The absA mutants in S. lividans 1326 (both DeltaabsA1 and DeltaabsA1A2) did not show early CDA production. On solid R2YE both MT1110Del
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9

Xue, Liu. "HIGH-ACTIVITY MUTANTS OF HUMAN BUTYRYLCHOLINESTERASE FOR COCAINE ABUSE TREATMENT." UKnowledge, 2013. http://uknowledge.uky.edu/pharmacy_etds/40.

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Cocaine is a widely abused drug without an FDA-approved medication. It has been recognized as an ideal anti-cocaine medication to accelerate cocaine metabolism producing biologically inactive metabolites via a route similar to the primary cocaine-metabolizing pathway, i.e. butyrylcholinesterase (BChE)-catalyzed hydrolysis. However, the native BChE has a low catalytic activity against cocaine. We recently designed and discovered a set of BChE mutants with a high catalytic activity specifically for cocaine. An ideal, therapeutically valuable mutant of human BChE should have not only a significan
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10

Merah, Mostefa. "Obtention et étude de mutants de Myxococcus xanthus affectés dans la production d'activités enzymatiques exocellulaires." Rouen, 1992. http://www.theses.fr/1992ROUES017.

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Myxococcus xanthus est une bactérie à gram-négatif qui sécrète de nombreuses activités enzymatiques durant la phase exponentielle de croissance. Nous avons tenté dans une première étape de cloner un gène de structure de l'une des trois activités sécrétées par cette bactérie: protéase, endoprotéase acide et glucanase en utilisant le promoteur mobile Ptac permettant de créer des phénotypes conditionnels inductibles par l'IPTG. Les résultats que nous avons obtenus ont montré que le clonage d'un fragment d'ADN de M xanthus situé en aval du Ptac dans E. Coli est faisable. Mais l'obtention condition
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11

Loureiro, Liliana Raquel Rodrigues. "DJ-1 mutants binding partners: insights into Parkinson's Disease." Master's thesis, Universidade de Aveiro, 2013. http://hdl.handle.net/10773/12583.

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Mestrado em Biotecnologia - Biotecnologia Molecular<br>Parkinson´s disease (PD), the second most common progressive neurodegenerative disorder, is a multifactorial disease caused by both genetic and environmental factors. Among the genes associated with PD, DJ-1 is a multifunctional protein involved in oxidative stress response and neuroprotection. DJ-1 mutations, such as L166P, M26I and E163K lead to loss of protein function causing early onset autosomal recessive PD. Moreover, the residue C106 is considered crucial in DJ-1 function as a sensor of oxidative stress. In this study, one missense
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12

Kottier, Sanneke Annet. "Investigation into the use of recombination for the production of site-specific mutants of coronavirus infectious bronchitis virus." Thesis, University of Reading, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.295313.

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13

Asfour, Hani. "Regulation of pyrimidine biosynthesis and virulence factor production in wild type, Pyr- and Crc- mutants in Pseudomonas aeruginosa." Thesis, University of North Texas, 2006. https://digital.library.unt.edu/ark:/67531/metadc5297/.

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Previous research in our laboratory established that pyrB, pyrC or pyrD knock-out mutants in Pseudomonas aeruginosa required pyrimidines for growth. Each mutant was also discovered to be defective in the production of virulence factors. Moreover, the addition of exogenous uracil did not restore the mutant to wild type virulence levels. In an earlier study using non-pathogenic P. putida, mutants blocked in one of the first three enzymes of the pyrimidine pathway produced no pyoverdine pigment while mutants blocked in the fourth, fifth or sixth steps produced copious quantities of pigment, just
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14

Wagner, Françoise. "Activités glutamate déshydrogénase et nitrate réductase du champignon ectomycorhizien Hebeloma cylindrosporum : étude de la variabilité génétique et production de mutants." Lyon 1, 1988. http://www.theses.fr/1988LYO19015.

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15

Wagner, Françoise. "Activités glutamate deshydrogénase et nitrate réductase du champignon ectomycorhizien Hebeloma cylindrosporum étude de la variabilité génétique et production de mutants /." Grenoble 2 : ANRT, 1988. http://catalogue.bnf.fr/ark:/12148/cb376191152.

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16

SANTANA, MARGARIDA. "La chaine respiratoire et la production d'atp chez bacillus subtilis. Caracterisation de mutants de la quinol oxydase et de l'atp synthase." Paris 11, 1995. http://www.theses.fr/1995PA112402.

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La chaine respiratoire de bacillus subtilis contient au moins quatre oxydases terminales. Deux de ces oxydases terminales, la quinol oxydase aa#3-600 et la cytochrome c oxydase caa#3-605 ont ete purifiees. Les genes de l'operon ctacdef qui codent les sous-unites de l'oxydase caa#3-605 ont ete isoles et sequences. Dans notre laboratoire, nous avons isole et sequence les genes de l'operon qoxabcd codant les sous-unites de l'oxydase aa#3-600. L'etude de l'expression des deux operons montre que l'expression de l'operon qoxabcd est constitutive, tandis que celle de l'operon ctacdef est dereprimee q
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17

Chambon, Christophe. "Mutants de saccharomyces cerevisiae bloqués dans l'activité farnesyl diphosphate synthetase : isolement, déterminismes génétique et physiologique de la production de terpènes aromatiques." Poitiers, 1990. http://www.theses.fr/1990POIT2326.

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Deux mutants de levure saccharomyces cerevisiae, vl134 et vl135, auxotrophes pour l'ergosterol et bloques dans l'activite farnesyl diphosphate (erg20) ont ete isoles. Les mutations affectant la farnesyl diphosphate synthetase des souches vl134 et vl135 ont ete notees respectivement erg20-1 et erg20-2. La particularite de ces souches mutantes est leur capacite a excreter des terpenes aromatiques (farnesol et geraniol dans le cas de vl134 et farnesol dans le cas de vl135). L'analyse genetique de ces souches a montre que d'autres mutations sur des genes de la voie de biosynthese de l'ergosterol e
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18

BOUMERDASSI, HASSINA. "Amelioration de la production de diacetyle chez lactococcus lactis subsp. Lactis biovar diacetylactis par modification des conditions de culture et selection de mutants." Massy, ENSIA, 1996. http://www.theses.fr/1996EIAA0061.

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La presente etude porte sur l'amelioration de la production de diacetyle par lactococcus lactis subsp. Lactis biovar diacetylactis. Dans la premiere partie, les effets du controle de la concentration en oxygene dissous ainsi que de l'addition de citrate dans le milieu ont ete etudies. L'oxygene augmente considerablement la production de diacetyle, dont la concentration maximale varie de 0,015 mm sous azote a 0,45 mm sous une pression de 2 atmospheres d'oxygene. L'augmentation de la production de diacetyle est probablement due a la decarboxylation chimique oxydative de l'acetolactate et a la di
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19

Janati-Idrissi, Rachid. "Utilisation de mutants pour l'étude du mécanisme de la fermentation acétono-butylique." Nancy 1, 1987. http://www.theses.fr/1987NAN10088.

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Sélection de mutants plus tolérants vis-à-vis du butanol et produisant plus de solvants, et de mutants ne produisant pas d'acétone en l'absence d'acide acétique dans le milieu. Étude des effets des acides acétique, butyrique et pyruvique sur la production de solvants et sur les activités de différentes enzymes (acétate kinase, butyrate kinase, coenzyme à transférase, acétoacétate décarboxylase,. . . ). Étude des effets du PH
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20

Kwon, Jong-Hee [Verfasser], Matthias [Gutachter] Rögner, and Mathias [Gutachter] Lübben. "Metabolic and fermentative analysis of cyanobacterial mutants for optimization of light-driven biohydrogen production / Jong-Hee Kwon ; Gutachter: Matthias Rögner, Mathias Lübben ; Fakultät für Biologie und Biotechnologie." Bochum : Ruhr-Universität Bochum, 2013. http://d-nb.info/1236813219/34.

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21

Matta-El-Ammouri, Ghassan. "Fermentation acétonobutylique : Obtention de mutants résistants au butanol, action des acides acétique et butyrique sur la formation de solvants." Nancy 1, 1986. http://www.theses.fr/1986NAN10079.

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Dans la fermentation acétonobutylique, la conversion du sucre en solvants est de l'ordre de 33 % ; le rapport habituel butanol/acétone/éthanol est 6/3/1/ ; la concentration maximale de glucose pouvant être dégradé est de 60 g/1 pour une teneur finale en solvants de 20 g/1. La principale limitation de cette fermentation réside dans une inhibition par les produits, en particulier le butanol, qui perturbe le métabolisme bactérien. Ce travail s'est focalisé sur l'obtention de mutants résistants au butanol, et sur le mécanisme d'action des acides acétique et butyrique dans la formation de solvants.
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22

Tailliez, Patrick. "Fermentation de la cellulose et de materiaux lignocellulosiques en ethanol par clostridium thermocellum : performances comparees de la souche sauvage et de mutants asporogenes et resistants a l'ethanol." Paris 7, 1988. http://www.theses.fr/1988PA077158.

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Isolement d'un mutant de clostridium thermocellum non producteur d'acide lactique en utilisant le fluoropyruvate comme agent de selection, pour empecher l'inhibition causee par cet acide et augmenter le rendement en ethanol. Clostridium thermocellum est capable de convertir en ethanol la lignocellulose pretraitee par hydrothermolyse ainsi que la cellulose pure
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23

Junelles, Anne-Marie. "Étude du mécanisme de la solvantogénèse chez clostridium acétobutylicum : Utilisation de mutants altérés dans le métabolisme de l'acétate et du butyrate." Nancy 1, 1988. http://www.theses.fr/1988NAN10161.

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24

Figarella, Araujo Katherine del Carmen. "Rolle der Prostaglandinproduktion in afrikanischen Trypanosomen Charakterisierung des Effektes von Prostaglandin D2 und seiner Metabolite auf die Blutform von Trypanosoma brucei = Role of prostaglandin production in African trypanosomes : Characterization of the effect of prostaglandin D2 and their metabolites in the bloodstream form of trypanosoma brucei /." [S.l. : s.n.], 2005.

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25

Andreani, Nadia Andrea. "INTO THE BLUE: Spoilage phenotypes of Pseudomonas fluorescens in food matrices." Doctoral thesis, Università degli studi di Padova, 2016. http://hdl.handle.net/11577/3424342.

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Spoilage induced by Pseudomonas strains is commonly found in a wide range of food products as a result of the ubiquitous presence of these strains and their ability to induce alteration through different mechanisms. Particular attention has been recently paid on those P. fluorescens strains able to induce a blue discolouration on several food matrices (e.g. dairy or meat products). Actually, poor data are available about this curious event that draw the attention of European consumer from 2010. In the present manuscript a step-by-step investigation of the spoilage potential of Pseudomonas fl
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26

Su, Hui Shan, and 蘇慧珊. "Oxytetracycline production by transformants and mutants of streptomyces rimosus." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/10487520507691630268.

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27

Lin, Ying-Zhen, and 林瑩禎. "Studies on L-tryptophan production by mutants of Brevibacterium divaricatum." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/79326417614756373626.

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28

Zhang, Shi-Feng, and 張士峰. "Studies on L-Lysine production by mutants of brevibacterium divaricatum." Thesis, 1990. http://ndltd.ncl.edu.tw/handle/59530839301447733299.

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29

CHAN, HUNG-DER, and 詹鴻得. "Isolation of Glucose derepressed yeast mutants and its amylase production." Thesis, 1992. http://ndltd.ncl.edu.tw/handle/76546254661120092631.

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30

Qiu, Zi Yi, and 邱紫怡. "Production of astaxanthin by phaffia rhodozyma over-producing mutants in batch fermentation." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/18221920978718248394.

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31

Lu, Yi-Hsueh, and 呂宜學. "Optimization of the fermentation production in Pichia pastoris and the function of rhodostomin mutants." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/62836972419668212888.

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碩士<br>國立成功大學<br>生物化學研究所<br>97<br>Integrins are a family of heterodimeric receptors and modulate many cellular processes, including growth, death, adhesion, and migration. Rhodostomin (Rho) is a potent platelet aggregation inhibitor and belongs to the family of disintegrins. Rho consists of 68 amino acids with a 48PRGDM53P motif and six disulfide bonds. In previous study we expressed Rho in Pichia pastrois (P. pastoris) and used it as the scaffold to design integrin αvβ3-specific drugs. In this study I optimized the fermentation culture condition to increase the protein production of Rho mutant
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32

Pereira, João Miguel Calmeiro. "Optogenetics and biotechnology : production and in vitro characterization of Ab-Initio designed channelrhodopsin-2 mutants." Master's thesis, 2015. http://hdl.handle.net/10316/30363.

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Dissertação de mestrado em Biotecnologia Farmacêutica, apresentada à Faculdade de Farmácia da Universidade de Coimbra<br>Nos últimos anos têm sido desenvolvidas várias ferramentas para permitir o controlo de neurónios específicos, possibilitando o estudo da sua função. Estas novas ferramentas superam a falta de selectividade e o fraco controlo temporal proveniente do uso de estimulação eléctrica no controlo de actividade neuronal. A optogenética refere-se á integração de óptica e genética para obter um ganho ou perda de função em eventos bem definidos dentro de células específicas em teci
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33

Couto, Márcia Regina Linhares. "Construction and validation of Escherichia coli mutants to improve the curcumin production by an engineered strain." Master's thesis, 2016. http://hdl.handle.net/1822/44771.

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Dissertação de mestrado em Bioengenharia<br>Curcumin has been reported for its beneficial therapeutic properties including as anti-cancer agent. However, it has poor bioavailability and it is quickly metabolized in the human body, implying a repetitive oral administration if a therapeutic effect is envisaged. Besides, its extraction from plants is very expensive. For these reasons, the use of microorganisms to produce it on large scale and with greater yields constitutes an interesting alternative. With this aim, Escherichia coli K-12 MG1655 (DE3) was previously engineered with three enzy
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34

Huang, Pei-Cheng, and 黃培城. "Characterization of Ralstonia solanacearum mutants with altered phenotypes on exopolysaccharide production, motility, biofilm formation or Arabidopsis pathogenesis." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/45997149144543922985.

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碩士<br>國立臺灣大學<br>植物科學研究所<br>96<br>Ralstonia solanacearum causes a deadly wilting disease on a wide range of crops. To achieve a successful infection, R. solanacearum is well equipped with many pathogenesis machineries, including swimming and twitching motility (MO), biofilm formation (BF), production of exopolysaccharides (EPS) and avoidance or suppression of host defenses. The general objective of this study is to characterize mutants of candidate genes involved in R. solanacearum virulence and fitness. Firstly, a group of transposon (Tn5)-insertional R. solanacearum mutants defective in EPS,
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35

Huang, Pei-Cheng. "Characterization of Ralstonia solanacearum mutants with altered phenotypes on exopolysaccharide production, motility, biofilm formation or Arabidopsis pathogenesis." 2008. http://www.cetd.com.tw/ec/thesisdetail.aspx?etdun=U0001-2507200813585000.

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36

Rosenberg, Jonathan. "Vitamin B6 Production in Bacillus subtilis." Doctoral thesis, 2018. http://hdl.handle.net/11858/00-1735-0000-002E-E32C-B.

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37

Du, Xuelian. "Characterization of the dinuclear metal center of Pyrococcus furiosus prolidase and production of its mutants with increased activity at low temperatures." 2006. http://www.lib.ncsu.edu/theses/available/etd-08082006-205833/unrestricted/etd.pdf.

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38

Chung, Hsin-Yu, and 鍾心語. "Functional characterization and pathogenicity of outer membrane vesicles isolated from Helicobacter pylori 26695 mutants defective in HP0859, CagA or VacA production." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/jz5sz5.

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39

Syu, Jau-Wei, and 徐晁偉. "NADPH reduction and H2 production of HydA-mutated-overexpressed Chlorella sp. DT mutants as well as generation of HydA-StrepII-overexpressed DT mutant." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/22980525229277648747.

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碩士<br>國立中興大學<br>生命科學系所<br>104<br>Green algae have a photosynthetic system that can produce H2 by hydrogenase (HydA, encoded by hydA) using sun light under certain condition. In the previous studies, an HydA-overexpressed mutant and some O2 tolerant HydA-mutated-overexpressed Chlorella sp. DT (DT) mutants were obtained to enhance the H2 production in this laboratory. However, the increased fold of HydA expression was not compatible to that of H2 production. In order to understand whether Ferredoxin-NADP+-oxidoreductase (FNR) competes electrons with HydA of DT mutants, NADPH reduction and H2 pho
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40

Hurley, James Franklin. "Disruption of Two Gene Loci Putatively Encoding Siderophore-Producing Nonribosomal Peptide Synthetases and Characterization of Siderophore Mutants." 2009. http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7410.

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The soil-borne, rhizosphere-competent, filamentous fungus Trichoderma virens is a well-known biocontrol agent able to control pathogenic fungi through the production of antibiotics, the induction of systemic resistance in host plants, or by directly parasitizing the competing fungus. Competition for iron is another means by which Trichoderma can hinder competing microorganisms, and siderophores are a means by which microorganisms obtain iron. In silico analysis of the T. virens genome suggested that two genes putatively encoding extracellular siderophore-producing nonribosomal peptide syntheta
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41

Cho, Kuan-Yin, and 卓冠吟. "Production of infectious bursal disease virus mutants with surficial histidine substitution and the effect of substitution on its binding ability to immobilized nickel ions." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/gz7u7f.

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碩士<br>國立中興大學<br>生物科技學研究所<br>106<br>Abstract Infectious Bursal Disease Virus (IBDV) mainly infects young chickens aged 3-6 weeks and pathologically causes necrosis of the B lymphocytes and suppression of the immune system. The infection is often accompanied by high morbidity and high mortality and so that often cause the poultry farmers suffering from huge economic loss. Recently, we concluded that the exposed residue His253 of a VP2-formed SVP is crucial for binding affinity of SVP to Ni-NTA. In order to effectively purify IBDV virus particles by IMAC, in this study, predict the exposure area
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