Bibliografías temáticas / Proteins maturation

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Literatura académica sobre el tema "Proteins maturation"

Autor: Grafiati
Publicado: 10 de marzo de 2023
Última modificación: 31 de julio de 2025

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Artículos de revistas sobre el tema "Proteins maturation"

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Hellen, Christopher U. T., and Eckard Wimmer. "Maturation of poliovirus capsid proteins." Virology 187, no. 2 (1992): 391–97. http://dx.doi.org/10.1016/0042-6822(92)90440-z.

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Sumi, Mamta P., and Arnab Ghosh. "Hsp90 in Human Diseases: Molecular Mechanisms to Therapeutic Approaches." Cells 11, no. 6 (2022): 976. http://dx.doi.org/10.3390/cells11060976.

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The maturation of hemeprotein dictates that they incorporate heme and become active, but knowledge of this essential cellular process remains incomplete. Studies on chaperon Hsp90 has revealed that it drives functional heme maturation of inducible nitric oxide synthase (iNOS), soluble guanylate cyclase (sGC) hemoglobin (Hb) and myoglobin (Mb) along with other proteins including GAPDH, while globin heme maturations also need an active sGC. In all these cases, Hsp90 interacts with the heme-free or apo-protein and then drives the heme maturation by an ATP dependent process before dissociating fro
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Garoff, Henrik, Roger Hewson, and Dirk-Jan E. Opstelten. "Virus Maturation by Budding." Microbiology and Molecular Biology Reviews 62, no. 4 (1998): 1171–90. http://dx.doi.org/10.1128/mmbr.62.4.1171-1190.1998.

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SUMMARY Enveloped viruses mature by budding at cellular membranes. It has been generally thought that this process is driven by interactions between the viral transmembrane proteins and the internal virion components (core, capsid, or nucleocapsid). This model was particularly applicable to alphaviruses, which require both spike proteins and a nucleocapsid for budding. However, genetic studies have clearly shown that the retrovirus core protein, i.e., the Gag protein, is able to form enveloped particles by itself. Also, budding of negative-strand RNA viruses (rhabdoviruses, orthomyxoviruses, a
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Remington, S. James. "Fluorescent proteins: maturation, photochemistry and photophysics." Current Opinion in Structural Biology 16, no. 6 (2006): 714–21. http://dx.doi.org/10.1016/j.sbi.2006.10.001.

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Matthews, Glenn. "Introduction: Proteolytic maturation of secretory proteins." Seminars in Cell & Developmental Biology 9, no. 1 (1998): 1–2. http://dx.doi.org/10.1006/scdb.1997.0193.

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Gross, I. "Regulation of fetal lung maturation." American Journal of Physiology-Lung Cellular and Molecular Physiology 259, no. 6 (1990): L337—L344. http://dx.doi.org/10.1152/ajplung.1990.259.6.l337.

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The recent identification of the genes for the surfactant proteins has greatly facilitated the study of the regulation of fetal lung alveolar epithelial cell development at the molecular level. In general, expression of the genes for the surfactant proteins is enhanced by the same hormones that stimulate phospholipid synthesis. There are, however, some notable differences that indicate that the genes for the different components of surfactant are independently regulated. Species differences in the response of the surfactant proteins to hormones such as glucocorticoids and adenosine 3',5'-cycli
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Roland, Mélanie, Jonathan Przybyla-Toscano, Florence Vignols, et al. "The plastidial Arabidopsis thaliana NFU1 protein binds and delivers [4Fe-4S] clusters to specific client proteins." Journal of Biological Chemistry 295, no. 6 (2020): 1727–42. http://dx.doi.org/10.1074/jbc.ra119.011034.

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Proteins incorporating iron–sulfur (Fe-S) co-factors are required for a plethora of metabolic processes. Their maturation depends on three Fe-S cluster assembly machineries in plants, located in the cytosol, mitochondria, and chloroplasts. After de novo formation on scaffold proteins, transfer proteins load Fe-S clusters onto client proteins. Among the plastidial representatives of these transfer proteins, NFU2 and NFU3 are required for the maturation of the [4Fe-4S] clusters present in photosystem I subunits, acting upstream of the high-chlorophyll fluorescence 101 (HCF101) protein. NFU2 is a
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Watanabe, Satoshi, Daisuke Sasaki, Taiga Tominaga, and Kunio Miki. "Structural basis of [NiFe] hydrogenase maturation by Hyp proteins." Biological Chemistry 393, no. 10 (2012): 1089–100. http://dx.doi.org/10.1515/hsz-2012-0197.

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Abstract [NiFe] hydrogenases catalyze reversible hydrogen production/consumption. The active site of [NiFe] hydrogenases contains a complex NiFe(CN)2CO center, and the biosynthesis/maturation of these enzymes is a complex and dynamic process, primarily involving six Hyp proteins (HypABCDEF). HypA and HypB are involved in the Ni insertion, whereas the other four Hyp proteins (HypCDEF) are required for the biosynthesis, assembly and insertion of the Fe(CN)2CO group. Over the last decades, a large number of functional and structural studies on maturation proteins have been performed, revealing de
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Davis, Brandi N., Aaron C. Hilyard, Giorgio Lagna, and Akiko Hata. "SMAD proteins control DROSHA-mediated microRNA maturation." Nature 454, no. 7200 (2008): 56–61. http://dx.doi.org/10.1038/nature07086.

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PAGES, Jean Marie, and Claude LAZDUNSKI. "Maturation of Exported Proteins in Escherichia coli." European Journal of Biochemistry 124, no. 3 (2005): 561–66. http://dx.doi.org/10.1111/j.1432-1033.1982.tb06630.x.

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Tesis sobre el tema "Proteins maturation"

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Gilker, Eva Adeline Gilker. "INTERACTIONS AND LOCALIZATION OF PROTEIN PHOSPHATASES, YWHA PROTEINS AND CELL CYCLE CONTROL PROTEINS IN MEIOSIS." Kent State University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=kent1532699317257539.

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Yarbrough, Daniel Kenneth. "Structural and mutational analysis of chromophore maturation in long wavelength fluorescent proteins /." view abstract or download file of text, 2004. http://wwwlib.umi.com/cr/uoregon/fullcit?p3120630.

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Thesis (Ph. D.)--University of Oregon, 2004.<br>Typescript. Includes vita and abstract. Includes bibliographical references (leaves 142-152). Also available for download via the World Wide Web; free to University of Oregon users.
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Meissner, Christina Sylvia. "Influence of HCMV proteins pUL71 and pUL77 on viral maturation." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2011. http://dx.doi.org/10.18452/16414.

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Die Bildung infektiöser Viruspartikel des humanen Zytomegalievirus (HCMV) ist ein mehr-stufiger Prozess. Sie beginnt mit der Verpackung der DNA in die Kapside im Kern, gefolgt von weiterer Reifung während des Transports durch das Zytoplasma und der abschließenden Freisetzung aus der Zelle. Im Zuge dieser Arbeit wurden zwei Proteine, die Einfluss auf die ebengenannten Prozesse haben, analysiert. Der erste Teil der Arbeit befasst sich mit der funktionellen Charakterisierung des HCMV Pro-teins pUL77. Es ist bekannt, dass das homologe Protein pUL25 in alpha-Herpesvirinae essentiell für die DNA-
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Atherton, Ruth Elizabeth. "Catalytic activity and maturation of the metalloprotease-disintegrin protein, MDC9 /." Access full-text from WCMC, 1999. http://proquest.umi.com/pqdweb?did=733095101&sid=8&Fmt=2&clientId=8424&RQT=309&VName=PQD.

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Cotta, Doné Stefania. "Nephrin - intracellular trafficking and podocyte maturation /." Stockholm, 2007. http://diss.kib.ki.se/2007/978-91-7357-411-2/.

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Tominaga, Taiga. "Structural studies on cyano group biosynthesis by [NiFe] hydrogenase maturation proteins." 京都大学 (Kyoto University), 2013. http://hdl.handle.net/2433/180637.

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Popescu, Costin-Ioan. "Maturation and processing of endogenous and viral proteins in the endoplasmic reticulum." Thesis, University of Oxford, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.422667.

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Hodson, D. J. "The RNA-binding proteins TIS11b and TIS11d regulate lymphocyte development and maturation." Thesis, University of Cambridge, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.604134.

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Over-expression studies in cultured B cells showed that both TIS11b and TIS11d were able to block plasma cell differentiation <i>in vitro</i>. This action was specific as class switch recombination was not affected. TIS11b conditional knockout mice showed normal lymphocyte development and maturation and a normal humoral immune response. TIS11b conditional knockout mice lacked marginal zone B cells and had reduced numbers of peritoneal B1 cells. However they mounted a normal humoral response to immunisation with NP-KLH, with normal affinity maturation and a normal memory response. Single knocko
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Lyall, Natalie. "The role of RAB2 in the maturation of macrophage phagosomes containing Candida albicans." Thesis, University of Aberdeen, 2018. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=238253.

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Phagosome maturation is a dynamic process involving the engulfment and degradation of pathogens by phagocytic cells. However, several pathogens have employed mechanisms that facilitate their survival and escape from the phagosome. The fungal pathogen, Candida albicans, is capable of switching from yeast to hyphal form to facilitate its pathogenicity and escape from the phagosome. Rab GTPases are key regulators in phagosome maturation by mediating interactions with the endocytic pathway and leading to biogenesis of the phagolysosome. The temporal localisation dynamics of Rab2 on maturing phagos
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Nangola, Sawitree. "The interference of human immunodeficiency virus assembly and maturation by ankyrin repeat proteins." Thesis, Paris 11, 2011. http://www.theses.fr/2011PA112044.

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Le but de ce travail est de découvrir des nouvelles protéines suceptibles d'interférer avec le cycle vital du virus HIV. De par leur repliement, les protéines à motifs ankyrines peuvent constituer une ossature protéique trés bien adaptée à cet objectif. Plusieurs interacteurs spécifiques de la protéine MA-CA du HIV ont été sélectionnées par exposition sur phage à partir d'une bibliothèque de variants d'ankyrines. Trois protéines isolées ont été produites à partir de clones ayant une forte activité de liaison. Le meilleur interacteur protéique (1D4) interagit avec un épitope situié sur le domai
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Libros sobre el tema "Proteins maturation"

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Workshop on Applied Molecular Evolution and the Maturation of the Immune Response (1989 Santa Fe, N.M.). Molecular evolution on rugged landscapes: Proteins, RNA, and the immune system : the proceedings of the Workshop on Applied Molecular Evolution and the Maturation of the Immune Response, held March, 1989 in Santa Fe, New Mexico. Edited by Perelson Alan S. 1947- and Kauffman Stuart A. Addison-Wesley Pub. Co., 1991.

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McEwen, Robert Kenneth. Characterisation of the maturation and secretion of the acid extracellular proteinase of Yarrowia Lipolytica. University of Birmingham, 1996.

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VanSlyke, Judy K. Proteolytic maturation of vaccinia virus structural proteins. 1992.

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Growth and Maturation Factors (Growth & Maturation Factors). John Wiley & Sons, 1985.

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Whitehead, Stephen S. Proteolytic maturation of Vaccinia virus structural proteins: Enzyme and substrate analysis. 1994.

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Lee, Peiyu. Proteolytic maturation of vaccinia virus virion-associated proteins: Analysis of substrate determinants. 1993.

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Suh, Kyungsun. Early events in the maturation of vesicular stomatitis virus G protein oligosaccharides. 1991.

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Luginbühl, Béatrice Barbara Cécile. Affinity maturation towards low picomolar anti-prion protein antibodies: Directed evolution of binding to conformationally unstable target proteins. 2006.

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Murphey, James Micheal. Soluble protein characteristics of Gewurztraminer and White Riesling grapes and wine during maturation. 1988.

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Biochemical, technological and structural changes in wheat proteins during grain maturation: Final technical report. Dept. of Biochemistry, Institute of Plant Biology, University of Agriculture, 1988.

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Capítulos de libros sobre el tema "Proteins maturation"

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Cooper, Trevor G. "Epididymal Proteins and Sperm Maturation." In Spermatogenesis — Fertilization — Contraception. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-662-02815-5_12.

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Cooper, Trevor G. "Epididymal Secretion and Resorption of Proteins." In The Epididymis, Sperm Maturation and Fertilisation. Springer Berlin Heidelberg, 1986. http://dx.doi.org/10.1007/978-3-642-71471-9_15.

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Fung, Jia Jun, Karla Blöcher-Juárez, and Anton Khmelinskii. "High-Throughput Analysis of Protein Turnover with Tandem Fluorescent Protein Timers." In Methods in Molecular Biology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-1732-8_6.

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AbstractTandem fluorescent protein timers (tFTs) are versatile reporters of protein dynamics. A tFT consists of two fluorescent proteins with different maturation kinetics and provides a ratiometric readout of protein age, which can be exploited to follow intracellular trafficking, inheritance and turnover of tFT-tagged proteins. Here, we detail a protocol for high-throughput analysis of protein turnover with tFTs in yeast using fluorescence measurements of ordered colony arrays. We describe guidelines on optimization of experimental design with regard to the layout of colony arrays, growth co
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Sripathy, K. V., and Steven P. C. Groot. "Seed Development and Maturation." In Seed Science and Technology. Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-19-5888-5_2.

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AbstractIn plants, a fascinating set of post-fertilization events result in the development of a dispersal unit known as a seed. During the maturation phase, seeds accumulate storage reserves and acquire desiccation tolerance, followed by an increase in seed vigour during maturation drying. Physiological (or mass) maturity may be attributed to the stage of seed maturation when maximum seed dry matter accumulation has occurred, marking the end of the seed-filling phase. The stage of maturity at harvest is one of the most important factors that can influence the quality of seeds. Recent studies
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Ghosh, Arnab, and Dennis J. Stuehr. "Hsp90 and Its Role in Heme-Maturation of Client Proteins: Implications for Human Diseases." In Heat Shock Proteins. Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-23158-3_12.

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Cuasnicú, Patricia S., Débora J. Cohen, Diego A. Ellerman, Dolores Busso, Vanina G. Da Ros, and Mauro M. Morgenfeld. "Changes in Specific Sperm Proteins During Epididymal Maturation." In The Epididymis: From Molecules to Clinical Practice. Springer US, 2002. http://dx.doi.org/10.1007/978-1-4615-0679-9_22.

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Robinson, Colin, Ian W. Brock, Laurence Hazell, and Andrew Hulford. "Transport and Maturation of Cytosolically Synthesised Thylakoid Proteins." In Research in Photosynthesis. Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-009-0383-8_31.

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Narayan, Mahesh. "The Case of Oxidative Folding of Ribonuclease A: Factors Impacting Fold Maturation of ER-Processed Proteins." In Folding of Disulfide Proteins. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-7273-6_2.

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Adiga, P. R., and C. V. Ramana Murty. "Vitamin Carrier Proteins During Embryonic Development in Birds and Mammals." In Ciba Foundation Symposium 98 - Molecular Biology of Egg Maturation. John Wiley & Sons, Ltd, 2008. http://dx.doi.org/10.1002/9780470720790.ch8.

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Kante, A., J. M. Berrez, and N. Latruffe. "Synthesis and Maturation of D-β-Hydroxybutyrate Dehydrogenase (BDH) from Mitochondrial Inner Membrane." In Dynamics of Membrane Proteins and Cellular Energetics. Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-73905-7_16.

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Actas de conferencias sobre el tema "Proteins maturation"

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Daufin, G., J. P. Escudier, H. Carrère, S. Bérot, L. Fillaudeau, and M. Decloux. "Application of Membrane Processes in Food and Dairy Industry." In CORROSION 2000. NACE International, 2000. https://doi.org/10.5006/c2000-00313.

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Abstract Membrane processes have become major tools in food processing for more than 25 years. The food industry represents a significant part of the turnover of the membrane manufacturing industry worldwide. The main applications of membrane operations are in the dairy industry (whey protein concentration, milk protein standardization, etc.) far before beverages (wine, beer, fruit juices, etc.) and egg products. Among the very numerous applications on an industrial scale a few striking particular separations which represent the last advances in food processing are reported. Clarification of f
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Savić, Željko, Aleksandar Čukić, Ljiljana Anđušić, and Božidar Milošević. "Changes of total proteins during maturation period of Sjenica cheese." In Zbornik radova 26. medunarodni kongres Mediteranske federacije za zdravlje i produkciju preživara - FeMeSPRum. Poljoprivredni fakultet Novi Sad, 2024. http://dx.doi.org/10.5937/femesprumns24028s.

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Proteins are one of the most important milk quality parameters and the course of coagulation, composition and technological properties of cheese curd depend on them. Proteins form the basis of the structure of Curds and cheese in which milk fat is incorporated, and represent the substrate for enzymes during the ripening of Sjenica cheese. Sjenica cheese is originally produced on the Sjenica-Pester plateau, from whole, fresh, sheep and cow milk, and belongs to the group of white cheeses in brine. Considering the importance of proteins, this research aimed to determine the protein content after
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Jackson, C. W., N. K. Hutson, and S. A. Steward. "CHANGES IN PROTEIN SYNTHESIS PROFILES OF MEGAKARYOCYTES (MK) DURING MATURATION." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643545.

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Several key differentiation events occur within the recognizable MK compartment; however, little is known about the macromolecular changes responsible for these events. In this study, protein synthesis profiles of morphologically immature and mature guinea pig MK populations have been analyzed by twodimensional gel electrophoresis after in vivo labeling with 35S-methionine. MK were enriched by a bovine plasma aggregation enrichment procedure (Blood 69:173, 1987) and then fractionated into immature and mature populations based on differences in their respective buoyant densities (Brit. J. Haema
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Schick, B. P., C. J. Walsh, and T. Jenkins-West. "CHANGES IN PROTEOGLYCAN AND SULFATED PROTEIN SYNTHESIS DURING MEGAKARYOCYTE MATURATION IN VIVO." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644620.

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We investigated changes in sulfated proteoglycan (PG) and sulfated protein synthesis during megakaryocyte (MK) maturation in vivo by characterizing the (35S)-labeled molecules in MKs and platelets (PLTs) obtained daily from 3 hr to 5 days after injection of guinea pigs with (35S)sulfate. Radioactivity in macromolecules was maximal in MKs 3 hr and in PLTs 3 days after the injection. The cells were solubilized in 8M urea/50mM Tris/0.2% Triton X-100/0.1M NaCl, and PGs and sulfoproteins were separated by DEAE-Sephacel chromatography. PGs (65% of cell 35s) were eluted as two fractions, one (PG-1, 8
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Ilina, E. L., A. S. Kiryushkin, E. D. Guseva, and K. N. Demchenko. "Methodological approaches to agrobacterium-mediated transformation of buckwheat (Fagopyrum esculentum Moench)." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.106.

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The method of Agrobacterium rhizogenes-mediated transformation of buckwheat has been established; composite plants have been obtained. The distribution of the cellular response to auxin by reporter proteins with different maturation times coincides.
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Renkl, A. C., J. Wussler, T. Ahrens, et al. "OPN INDUCES DENDRITIC CELL (DC) ACTIVATION AND POLARIZES THEIR MATURATION TOWARDS A DC1 PHENOTYPE." In 3rd International Conference on Osteopontin and SIBLING (Small Integrin-Binding Ligand, N-linked Glycoprotein) Proteins, 2002. TheScientificWorld Ltd, 2002. http://dx.doi.org/10.1100/tsw.2002.312.

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Cramer, Elisabeth M., F. John, William Vainchenker, and Janine Breton-Gorius. "PRODUCTION AND LOCALISATION OF ALPHA-GRANULE PROTEINS IN MATURING MEGAKARYOCYTES: AN OVERVIEW ON ULTRA-STRUCTURAL ASPECTS OF MEGAKARYOCYTE MATURATION." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642952.

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In order to study the production of α- granule proteins in maturing megakaryocytes, we used immunocytochemical techniques performed on cultured and enriched bone marrow megakaryocytes. Cultures were prepared from bone marrow CFU-MK with the methylcellulose and plasma clot techniques. Preparation of bone marrow megakaryocytes was carried out from human or pig rib marrow separated on percoll gradient and counterflow centrifugation. Megakaryocyte preparations were 90$ pure and represented 85$ of those in the whole marrow. Activation was prevented with prostacyclin and prefixation with low concent
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Moore, John J., Robert M. Moore, Deepak Kumar, et al. "Differential Expression of Fibulin Family Proteins in Mechanically Strong vs. Weak Fetal Membrane Fragments." In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-175332.

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Untimely rupture of the fetal membranes (FM), the amnion and choriodecidua, which normally surround and protect the fetus prior to delivery, is a major cause of preterm birth and results in significant infant mortality and morbidity. The physiological mechanism which normally leads the FM to weaken and fail prior to birth is not known. Conventional thinking that FM rupture is precipitated by the stress of uterine contractions during labor fails to explain the 10% of term deliveries and 40% of preterm deliveries in which FM rupture is the sentinel event, preceding any uterine contractions. Rece
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Colman, R. W., A. Gewirtz, D. L. Wang, et al. "BIOSYNTHESIS AND EXPRESSION OF FACTOR V IN MAGAKARYOCYTES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642955.

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Coagulation factor V (FV), is a single chain, multifunctional glycoprotein of Mr 350,000 which interacts with a variety of hemostatic proteins such as factor Xa, prothrombin, thrombin and protein C, on the surface of platelets and vascular endothelial cells. FV serves as both a cofactor and substrate in the generation of thrombin and plays a critical regulatory role in both physiologic hemostasis and pathologic thrombosis. The biosynthesis of FV and its subsequent expression are therefore expected to be precisely controlled and may differ in the three sites of synthesis - hepatocytes, endothel
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Batista, Michael, Hadi T. Nia, Karen Cox, et al. "Effects of Chondroadherin on Cartilage Nanostructure and Biomechanics via Murine Model." In ASME 2013 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/sbc2013-14516.

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While small leucine rich proteins/proteoglycans (SLRPs) are present in very low concentrations in the extracellular matrix (ECM), they have been shown to be critical determinants of the proper ECM assembly and function in connective tissues [1] including bone [2], cornea [3], and cartilage [4]. However, their direct and indirect roles in matrix biomechanics and the potential for osteoarthritis-related dysfunction of cartilage remain unclear. With the advent of new high resolution nanotechnological tools, the direct quantification of cartilage biomechanical properties using murine models can pr
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Informes sobre el tema "Proteins maturation"

1

Theg, Steven. Targeting Maturation and Quality Control of Photosynthetic Membrane Proteins. Office of Scientific and Technical Information (OSTI), 2018. http://dx.doi.org/10.2172/1457570.

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Galili, Gad, and Alan Bennett. Role of Molecular Chaperone in Wheat Storage Protein Assembly. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7604926.bard.

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Following sequestration into the ER, wheat gliadins assemble into complexes that initiate the formation of protein bodies. In the present work we have characterized the DNA sequence and regulation of expression of a plant BiP and also studied its interaction with wheat storage proteins as well as its role in the maturation of these storage proteins. In the Israeli lab, immunoprecipitation studies were made using anti BiP and anti storage proteins sera, both in wheat and in transgenic tobacco plants expressing a wheat gliadin storage proteins. In both cases, we could show that BiP interacts wit
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3

Schuster, Gadi, and David Stern. Integrated Studies of Chloroplast Ribonucleases. United States Department of Agriculture, 2011. http://dx.doi.org/10.32747/2011.7697125.bard.

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Gene regulation at the RNA level encompasses multiple mechanisms in prokaryotes and eukaryotes, including splicing, editing, endo- and exonucleolytic cleavage, and various phenomena related to small or interfering RNAs. Ribonucleases are key players in nearly all of these post-transcriptional mechanisms, as the catalytic agents. This proposal continued BARD-funded research into ribonuclease activities in the chloroplast, where RNase mutation or deficiency can cause metabolic defects and is often associated with plant chlorosis, embryo or seedling lethality, and/or failure to tolerate nutrient
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4

Blumwald, Eduardo, and Avi Sadka. Citric acid metabolism and mobilization in citrus fruit. United States Department of Agriculture, 2007. http://dx.doi.org/10.32747/2007.7587732.bard.

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Accumulation of citric acid is a major determinant of maturity and fruit quality in citrus. Many citrus varieties accumulate citric acid in concentrations that exceed market desires, reducing grower income and consumer satisfaction. Citrate is accumulated in the vacuole of the juice sac cell, a process that requires both metabolic changes and transport across cellular membranes, in particular, the mitochondrial and the vacuolar (tonoplast) membranes. Although the accumulation of citrate in the vacuoles of juice cells has been clearly demonstrated, the mechanisms for vacuolar citrate homeostasi
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5

Flaishman, Moshe, Herb Aldwinckle, Shulamit Manulis, and Mickael Malnoy. Efficient screening of antibacterial genes by juvenile phase free technology for developing resistance to fire blight in pear and apple trees. United States Department of Agriculture, 2008. http://dx.doi.org/10.32747/2008.7613881.bard.

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Objectives: The original objectives of this project were to: Produce juvenile-free pear and apple plants and examine their sensitivity to E. amylovora; Design novel vectors, for antibacterial proteins and promoters expression, combined with the antisense TFL1 gene, and transformation of Spadona pear in Israel and Galaxy apple in USA. The original objectives were revised from the development of novel vectors with antibacterial proteins combined with the TFL-1 due to the inefficiency of alternative markes initially evaluated in pear, phoshomannose-isomerase and 2-deoxyglucose-6-phosphate phospha
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6

Swartz, James. Using in vitro maturation and cell-free expression to explore [FeFe] hydrogenase activation and protein scaffolding requirements. Office of Scientific and Technical Information (OSTI), 2017. http://dx.doi.org/10.2172/1341627.

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Funkenstein, Bruria, and Cunming Duan. GH-IGF Axis in Sparus aurata: Possible Applications to Genetic Selection. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7580665.bard.

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Many factors affect growth rate in fish: environmental, nutritional, genetics and endogenous (physiological) factors. Endogenous control of growth is very complex and many hormone systems are involved. Nevertheless, it is well accepted that growth hormone (GH) plays a major role in stimulating somatic growth. Although it is now clear that most, if not all, components of the GH-IGF axis exist in fish, we are still far from understanding how fish grow. In our project we used as the experimental system a marine fish, the gilthead sea bream (Sparus aurata), which inhabits lagoons along the Mediter
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8

Firon, Nurit, Prem Chourey, Etan Pressman, Allen Hartwell, and Kenneth J. Boote. Molecular Identification and Characterization of Heat-Stress-Responsive Microgametogenesis Genes in Tomato and Sorghum - A Feasibility Study. United States Department of Agriculture, 2007. http://dx.doi.org/10.32747/2007.7591741.bard.

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Exposure to higher than optimal temperatures - heat-stress (HS) - is becoming increasingly common to all crop plants worldwide. Heat stress coinciding with microgametogenesis, especially during the post-meiotic phase that is marked by starch biosynthesis, is often associated with starch-deficient pollen and male sterility and ultimately, greatly reduced crop yields. The molecular basis for the high sensitivity of developing pollen grains, on one hand, and factors involved in pollen heat-tolerance, on the other, is poorly understood. The long-term goal of this project is to provide a better und
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9

Gafni, Yedidya, Moshe Lapidot, and Vitaly Citovsky. Dual role of the TYLCV protein V2 in suppressing the host plant defense. United States Department of Agriculture, 2013. http://dx.doi.org/10.32747/2013.7597935.bard.

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TYLCV-Is is a major tomato pathogen, causing extensive crop losses in Israel and the U.S. We have identified a TYLCV-Is protein, V2, which acts as a suppressor of RNA silencing. Intriguingly, the counter-defense function of V2 may not be limited to silencing suppression. Our recent data suggest that V2 interacts with the tomato CYP1 protease. CYP1 belongs to the family of papain-like cysteine proteases which participate in programmed cell death (PCD) involved in plant defense against pathogens. Based on these data we proposed a model for dual action of V2 in suppressing the host antiviral defe
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10

Bostock, Richard M., Dov Prusky, and Martin Dickman. Redox Climate in Quiescence and Pathogenicity of Postharvest Fungal Pathogens. United States Department of Agriculture, 2003. http://dx.doi.org/10.32747/2003.7586466.bard.

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Monilinia fructicola causes brown rot blossom blight and fruit rot in stone fruits. Immature fruit are highly resistant to brown rot but can become infected. These infections typically remain superficial and quiescent until they become active upon maturation of the fruit. High levels of chlorogenic acid (CGA) and related compounds occur in the peel of immature fruit but these levels decline during ripening. CGA inhibits cutinase expression, a putative virulence factor, with little or no effect on spore germination or hyphal growth. To better understand the regulation of cutinase expression by
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