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Literatura académica sobre el tema "Pseudotype"

Autor: Grafiati

Publicado: 14 de marzo de 2023

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Artículos de revistas sobre el tema "Pseudotype"

1

Chan, Eva, Gabrielle Heilek-Snyder, Nick Cammack, Surya Sankuratri y Changhua Ji. "Development of a Moloney Murine Leukemia Virus-Based Pseudotype Anti-HIV Assay Suitable for Accurate and Rapid Evaluation of HIV Entry Inhibitors". Journal of Biomolecular Screening 11, n.º 6 (7 de junio de 2006): 652–63. http://dx.doi.org/10.1177/1087057106288881.

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There has been increasing interest in the identification of novel HIV entry inhibitors. For the discovery of these entry inhibitors, robust surrogate anti-HIV assays are highly desired. The authors report a novel anti-HIV assay system using Moloney murine leukemia viruses (MMLVs) pseudotyped with cytoplasmic tail-truncated HIV envelope protein gp140. These pseudotyped MMLV-HIVgp140 viral particles carry luciferase transcripts; therefore, robust luciferase signal can be detected in cells infected by these pseudotypes. Polycationic agent polybrene and spinoculation markedly enhanced the infection efficiency of these pseudotypes. It was demonstrated that the tropism of these pseudotypes is dependent on the pseudotyped HIV envelope proteins. MMLV viruses pseudotyped with gp140 from an R5 HIV virus specifically infect CCR5-expressing cells, and viruses pseudotyped with gp140 from an X4 HIV virus specifically infect CXCR4-expressing cells. Furthermore, CCR5 antagonists inhibited only MMLV-gp140(R5) infections, and CXCR4 antagonists inhibited only MMLV-gp140(X4) infections. A variety of known HIV entry inhibitors were tested in both R5- and X4-dependent pseudotype antiviral assays, and the IC50 values generated were consistent with published results. The pseudotype antiviral assay was also used in the characterization of hundreds of novel CCR5 antagonists. The IC50 values determined in this assay were compared with those determined in HIV antiviral and cell-cell fusion (CCF) assays, and good correlation was found between pseudotype antiviral assay and HIV antiviral assay ( R2 = 0.9) or CCF assay ( R2 = 0.8).

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2

Bruett, Linda y Janice E. Clements. "Functional Murine Leukemia Virus Vectors Pseudotyped with the Visna Virus Envelope Show Expanded Visna Virus Cell Tropism". Journal of Virology 75, n.º 23 (1 de diciembre de 2001): 11464–73. http://dx.doi.org/10.1128/jvi.75.23.11464-11473.2001.

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ABSTRACT Pseudotype virus vectors serve as a powerful tool for the study of virus receptor usage and entry. We describe the development of murine leukemia virus (MuLV) particles pseudotyped with the visna virus envelope glycoprotein and encoding a green fluorescent protein reporter as a tool to study the expression of the visna virus receptor. Functional MuLV/visna virus pseudotypes were obtained when the cytoplasmic tail of the visna virus envelope TM protein was truncated to 3, 7, or 11 amino acids in length. MuLV/visna virus particles were used to transduce a panel of cell types from various organisms, including sheep, goat, human, hamster, mouse, monkey, and quail. The majority of the cells examined were susceptible to MuLV/visna pseudotype viruses, supporting the notion that the visna virus cellular receptor is a widely expressed protein found in many species. Of 16 different cell types tested, only mouse embryo fibroblast NIH 3T3 cells, hamster ovary CHO cells, and the human promonocyte cell line U937 cells were not susceptible to transduction by the pseudotyped virus. The production of functional MuLV/visna virus pseudotypes has provided a sensitive, biologically relevant system to study visna virus cell entry and envelope-receptor interactions.

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3

Poluri, Ananthalakshmi, Rebecca Ainsworth, Scott C. Weaver y Richard E. Sutton. "Functional Pseudotyping of Human Immunodeficiency Virus Type 1 Vectors by Western Equine Encephalitis Virus Envelope Glycoprotein". Journal of Virology 82, n.º 24 (8 de octubre de 2008): 12580–84. http://dx.doi.org/10.1128/jvi.01503-08.

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ABSTRACT We investigated the ability of western equine encephalitis virus envelope glycoproteins (WEEV GP) to pseudotype lentiviral vectors. The titers of WEEV GP-pseudotyped human immunodeficiency virus type 1 (HIV) ranged as high as 8.0 × 104 IU/ml on permissive cells. Sera from WEEV-infected mice specifically neutralized these pseudotypes; cell transduction was also sensitive to changes in pH. The host range of the pseudotyped particles in vitro was somewhat limited, which is atypical for most alphaviruses. HIV vectors pseudotyped by WEEV GP may be a useful tool for characterizing WEEV cell binding and entry and screening for small-molecule inhibitors.

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4

Codran, Audrey, Cathy Royer, Daniel Jaeck, Michèle Bastien-Valle, Thomas F. Baumert, Marie Paule Kieny, Carlos Augusto Pereira y Jean-Pierre Martin. "Entry of hepatitis C virus pseudotypes into primary human hepatocytes by clathrin-dependent endocytosis". Journal of General Virology 87, n.º 9 (1 de septiembre de 2006): 2583–93. http://dx.doi.org/10.1099/vir.0.81710-0.

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Hepatitis C virus (HCV) is a major cause of chronic hepatitis worldwide. Studies of the early steps of HCV infection have been hampered by the lack of convenient in vitro or in vivo models. Although several cell-surface molecules that mediate the binding of HCV envelope proteins to target cells have been identified, mechanisms of viral entry into human hepatocytes are still poorly understood. Vesicular stomatitis virus/HCV pseudotyped viruses expressing the HCV envelope glycoproteins on the viral envelope were generated and it was found that their entry into human hepatocytes required co-expression of E1 and E2 on the pseudotype surface. Neutralization of pseudotype infection by anti-HCV antibodies suggested that cellular entry was mediated by HCV envelope glycoproteins and by previously characterized cell-surface molecules, including CD81. An entry assay based on the release of a fluorochrome from labelled HCV pseudotypes provided evidence for a pH-dependent fusion of the pseudotype envelope with a cellular compartment. By using a panel of endocytosis inhibitors, it is postulated that penetration of HCV into primary cultures of hepatocytes takes place by clathrin-mediated endocytosis.

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5

Kretschmer, Maibritt, Patrycja Kadlubowska, Daniel Hoffmann, Birco Schwalbe, Heidi Auerswald y Michael Schreiber. "Zikavirus prME Envelope Pseudotyped Human Immunodeficiency Virus Type-1 as a Novel Tool for Glioblastoma-Directed Virotherapy". Cancers 12, n.º 4 (18 de abril de 2020): 1000. http://dx.doi.org/10.3390/cancers12041000.

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Glioblastoma multiforme is the most lethal type of brain tumor that is not yet curable owing to its frequent resurgence after surgery. Resistance is mainly caused by the presence of a subpopulation of tumor cells, the glioma stem cells (GSCs), which are highly resistant to radiation and chemotherapy. In 2015, Zikavirus (ZIKV)-induced microcephaly emerged in newborns, indicating that ZIKV has a specific neurotropism. Accordingly, an oncolytic tropism for infecting GSCs was demonstrated in a murine tumor model. Like other flaviviruses, ZIKV is enveloped by two proteins, prM and E. The pME expression plasmid along with the HIV-1 vector pNL Luc AM generated prME pseudotyped viral particles. Four different prME envelopes, Z1 to Z4, were cloned, and the corresponding pseudotypes, Z1- to Z4-HIVluc, produced by this two-plasmid system, were tested for entry efficiency using Vero-B4 cells. The most efficient pseudotype, Z1-HIVluc, also infected glioma-derived cell lines U87 and 86HG39. The pseudotype system was then extended by using a three-plasmid system including pME-Z1, the HIV-1 packaging plasmid psPAX2, and the lentiviral vector pLenti-luciferase-P2A-Neo. The corresponding pseudotype, designated Z1-LENTIluc, also infected U87 and 86HG39 cells. Altogether, a pseudotyped virus especially targeting glioma-derived cells might be a promising candidate for a prospective glioblastoma-directed virotherapy.

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6

Spiegel, Martin, Michael Bitzer, Andrea Schenk, Heidi Rossmann, Wolfgang J. Neubert, Ursula Seidler, Michael Gregor y Ulrich Lauer. "Pseudotype Formation of Moloney Murine Leukemia Virus with Sendai Virus Glycoprotein F". Journal of Virology 72, n.º 6 (1 de junio de 1998): 5296–302. http://dx.doi.org/10.1128/jvi.72.6.5296-5302.1998.

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ABSTRACT Mixed infection of cells with both Moloney murine leukemia virus (MoMLV) and related or heterologous viruses produces progeny pseudotype virions bearing the MoMLV genome encapsulated by the envelope of the other virus. In this study, pseudotype formation between MoMLV and the prototype parainfluenza virus Sendai virus (SV) was investigated. We report for the first time that SV infection of MoMLV producer cells results in the formation of MoMLV(SV) pseudotypes, which display a largely extended host range compared to that of MoMLV particles. This could be associated with SV hemagglutinin-neuraminidase (SV-HN) glycoprotein incorporation into MoMLV envelopes. In contrast, solitary incorporation of the other SV glycoprotein, SV fusion protein (SV-F), resulted in a distinct and narrow extension of the MoMLV host range to asialoglycoprotein receptor (ASGP-R)-positive cells (e.g., cultured human hepatoma cells). Since stably ASGP-R cDNA-transfected MDCK cells, but not parental ASGP-R-negative MDCK cells, were found to be transduced by MoMLV(SV-F) pseudotypes and transduction of ASGP-R-expressing cells was found to be inhibited by ASGP-R antiserum, a direct proof for the ASGP-R-restricted tropism of MoMLV(SV-F) pseudotypes was provided. Cultivation of ASGP-R-positive HepG2 hepatoma cells on Transwell-COL membranes led to a significant enhancement of MoMLV(SV-F) titers in subsequent flowthrough transduction experiments, thereby suggesting the importance of ASGP-R accessibility at the basolateral domain for MoMLV(SV-F) pseudotype transduction. The availability of such ASGP-R-restricted MoMLV(SV-F)-pseudotyped vectors opens up new perspectives for future liver-restricted therapeutic gene transfer applications.

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7

Meyer, Keith, Aster Beyene, Terry L. Bowlin, Arnab Basu y Ranjit Ray. "Coexpression of Hepatitis C Virus E1 and E2 Chimeric Envelope Glycoproteins Displays Separable Ligand Sensitivity and Increases Pseudotype Infectious Titer". Journal of Virology 78, n.º 23 (1 de diciembre de 2004): 12838–47. http://dx.doi.org/10.1128/jvi.78.23.12838-12847.2004.

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ABSTRACT We have previously reported that a pseudotype virus generated by reconstitution of hepatitis C virus (HCV) chimeric envelope glycoprotein E1-G or E2-G on the surface of a temperature-sensitive mutant of vesicular stomatitis virus (VSVts045) interacts independently with mammalian cells to initiate infection. Here, we examined whether coexpression of both of the envelope glycoproteins on pseudotype particles would augment virus infectivity and/or alter the functional properties of the individual subunits. Stable transfectants of baby hamster kidney (BHK) epithelial cells expressing either one or both of the chimeric envelope glycoproteins of HCV on the cell surface were generated. The infectious titer of the VSV pseudotype, derived from a stable cell line incorporating both of the chimeric glycoproteins of HCV, was ∼4- to 5-fold higher than that of a pseudotype bearing E1-G alone or ∼25- to 30-fold higher than that of E2-G alone when assayed with a number of mammalian cell lines. Further studies suggested that that the E1-G/E2-G or E2-G pseudotype was more sensitive to the inhibitory effect of heparin than the E1-G pseudotype. Treatment of the E1-G/E2-G pseudotype with a negatively charged sulfated sialyl lipid (NMSO3) displayed a ∼4-fold-higher sensitivity to neutralization than pseudotypes with either of the two individual glycoproteins. In contrast, VSVts045, used as a backbone for the generation of pseudotypes, displayed at least 20-fold-higher sensitivity to NMSO3-mediated inhibition of virus plaque formation. The effect of low-density lipoprotein on the E1-G pseudotype was greater than that apparent for the E1-G/E2-G pseudotype. The treatment of cells with monoclonal antibodies to CD81 displayed an inhibitory effect upon the pseudotype with E1-G/E2-G or with E2-G alone. Taken together, our results indicate that the HCV E1 and E2 glycoproteins have separable functional properties and that the presence of these two envelope glycoproteins on VSV/HCV pseudotype particles increases infectious titer.

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8

Pöhlking, Celine, Sebastian Beier, Jan Patrick Formanski, Michael Friese, Michael Schreiber y Birco Schwalbe. "Isolation of Cells from Glioblastoma Multiforme Grade 4 Tumors for Infection with Zika Virus prME and ME Pseudotyped HIV-1". International Journal of Molecular Sciences 24, n.º 5 (24 de febrero de 2023): 4467. http://dx.doi.org/10.3390/ijms24054467.

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This study aimed to isolate cells from grade 4 glioblastoma multiforme tumors for infection experiments with Zika virus (ZIKV) prME or ME enveloped HIV-1 pseudotypes. The cells obtained from tumor tissue were successfully cultured in human cerebrospinal fluid (hCSF) or a mixture of hCSF/DMEM in cell culture flasks with polar and hydrophilic surfaces. The isolated tumor cells as well as the U87, U138, and U343 cells tested positive for ZIKV receptors Axl and Integrin αvβ5. Pseudotype entry was detected by the expression of firefly luciferase or green fluorescent protein (gfp). In prME and ME pseudotype infections, luciferase expression in U-cell lines was 2.5 to 3.5 logarithms above the background, but still two logarithms lower than in the VSV-G pseudotype control. Infection of single cells was successfully detected in U-cell lines and isolated tumor cells by gfp detection. Even though prME and ME pseudotypes had low infection rates, pseudotypes with ZIKV envelopes are promising candidates for the treatment of glioblastoma.

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9

Sandrin, Virginie, Delphine Muriaux, Jean-Luc Darlix y François-Loïc Cosset. "Intracellular Trafficking of Gag and Env Proteins and Their Interactions Modulate Pseudotyping of Retroviruses". Journal of Virology 78, n.º 13 (1 de julio de 2004): 7153–64. http://dx.doi.org/10.1128/jvi.78.13.7153-7164.2004.

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ABSTRACT Glycoproteins derived from most retroviruses and from several families of enveloped viruses can form infectious pseudotypes with murine leukemia virus (MLV) and lentiviral core particles, like the MLV envelope glycoproteins (Env) that are incorporated on either virus type. However, coexpression of a given glycoprotein with heterologous core proteins does not always give rise to highly infectious viral particles, and restrictions on pseudotype formation have been reported. To understand the mechanisms that control the recruitment of viral surface glycoproteins on lentiviral and retroviral cores, we exploited the fact that the feline endogenous retrovirus RD114 glycoprotein does not efficiently pseudotype lentiviral cores derived from simian immunodeficiency virus, whereas it is readily incorporated onto MLV particles. Our results indicate that recruitment of glycoproteins by the MLV and lentiviral core proteins occurs in intracellular compartments and not at the cell surface. We found that Env and core protein colocalization in intracytoplasmic vesicles is required for pseudotype formation. By investigating MLV/RD114 Env chimeras, we show that signals in the cytoplasmic tail of either glycoprotein differentially influenced their intracellular localization; that of MLV allows endosomal localization and hence recruitment by both lentiviral and MLV cores. Furthermore, we found that upon membrane binding, MLV core proteins could relocalize Env glycoproteins in late endosomes and allow their incorporation on viral particles. Thus, intracellular colocalization, as well as interactions between Env and core proteins, may influence the recruitment of the glycoprotein onto viral particles and generate infectious pseudotyped viruses.

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10

Balliet, John W. y Paul Bates. "Efficient Infection Mediated by Viral Receptors Incorporated into Retroviral Particles". Journal of Virology 72, n.º 1 (1 de enero de 1998): 671–76. http://dx.doi.org/10.1128/jvi.72.1.671-676.1998.

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ABSTRACT Many host cell surface proteins, including viral receptors, are incorporated into enveloped viruses. To address the functional significance of these host proteins, murine leukemia viruses containing the cellular receptors for Rous sarcoma virus (Tva) or ecotropic murine leukemia virus (MCAT-1) were produced. These receptor-pseudotyped viruses efficiently infect cells expressing the cognate viral envelope glycoproteins, with titers of up to 105 infectious units per milliliter for the Tva pseudotypes. Receptor and viral glycoprotein specificity and functional requirements are maintained, suggesting that receptor pseudotype infection recapitulates events of normal viral entry. The ability of the Tva and MCAT-1 pseudotypes to infect cells efficiently suggests that, in contrast to human immunodeficiency virus type 1 entry, neither of these retroviral receptors requires a coreceptor for membrane fusion. In addition, the ability of receptor pseudotypes to target infected cells suggests that they may be useful therapeutic reagents for directing infection of viral vectors. Receptor-pseudotyped viruses may be useful for identifying new viral receptors or for defining functional requirements of known receptors. Moreover, this work suggests that the production of receptor pseudotypes in vivo could provide a mechanism for expanded viral tropism with potential effects on the pathogenesis and evolution of the virus.

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Tesis sobre el tema "Pseudotype"

1

Strang, Blair Lewis. "Development and characterisation of pseudotype HIV vectors". Thesis, University College London (University of London), 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.406402.

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2

Piccini, Giulia. "Evaluation of Influenza Lentiviral Pseudotypes as an alternative source of antigen in the Neuraminidase Inhibition Enzyme-Linked Lectin Assay". Doctoral thesis, Università di Siena, 2020. http://hdl.handle.net/11365/1096232.

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The continuously changing epidemiology of influenza viruses needs to be yearly addressed with a fast and massive production of vaccines that prevent epidemic outbreaks and occasional pandemics worldwide. Currently licensed flu vaccines are designed to elicit antibodies against the viral hemagglutinin (HA). Formulations containing standardized amounts of neuraminidase (NA) are however gaining growing interest in the influenza vaccine field, as NA‐inhibiting (NI) antibodies have been associated with resistance to disease as well as reduced severity and duration of illness. The Neuraminidase Inhibition Enzyme-Linked Lectin Assay (NI-ELLA), which quantifies NI antibody titers based on the ability of specific antibodies to inhibit NA activity, is regarded as a reliable and high-throughput method to measure NI antibodies in human sera. To avoid the non-specific inhibition of HA-binding antibodies that may be present in serum samples, viruses containing an antigenically mismatched HA (reassortants) or Triton-X100(Tx)-treated wild-type (WT) viruses that retain NA functionality have been employed as NA source in the ELLA assay. An innovative approach that exploits NA-bearing pseudotypes (or pseudoviruses, PV) as surrogate viruses expressing the NA of interest has been recently investigated to provide a safer product to be used for the ELLA test. The first aim of this PhD work was to evaluate three different sources of NA (reassortant viruses, Tx-inactivated WT viruses, and lentiviral PV) to compare their ability in the measurement of NI-ELLA titers. In order to do that, two independent panels of sera were analyzed by ELLA assay to investigate the antibody response against influenza NA subtype N1 (A/California/07/2009 (H1N1pdm)) and N2 (A/Hong Kong/4801/2014 (H3N2)). The NI titers were measured either as the 50% endpoint or 50% inhibitory concentration (IC50) and were compared for every source of antigen. The linear regression, Spearman rank, Bland-Altman and Intraclass Correlation (ICC) analyses were employed to assess the comparability of the data obtained. Analysis of results showed that the ELLA performed well with all three sources of NA. The correlation coefficients (r2) for all comparisons were high (r2 > 0.92) when titers were reported either as endpoint or as IC50. Comparisons that included PV as antigen source demonstrated the best concordance, with 90% of N1 titers and 88% of N2 titers measured by the pseudotypes-based ELLA (PV-ELLA) showing less than 2-fold difference compared to those obtained in the assays performed with the other sources of NA. ICC results showed a high concordance between the different methods when either examining N1 or N2 titers (ICC coefficients of 0.861 and 0.817, respectively). Bland-Altman analyses revealed an overall agreement, and showed that titers measured against N2 antigens (reassortant H6N2 and H11N2 PV) were in better agreement than N1 titers measured using reassortant H6N1 and H11N1 PV antigens. For both anti-N1 and anti-N2 titers, the assays performed with the available sources of antigen show similar ability to detect NI titers ≥40, which have recently been associated with protection against influenza. The different ELLA platforms show comparable Geometric Mean Titer (GMT) values as well as similar titers range, suggesting the suitability of performing ELLA with each of the NA sources evaluated. The second aim of this project was to validate the PV-ELLA executed by using H11N1ACal/09 and H11N2HK/14 PV, following the recommendations provided by international guidelines. Both the ELLA performed with H11N2HK/14 PV and H11N1ACal/09 PV fulfill the pre-established criteria for definition of assay specificity, as the titer raised by the high positive serum sample against the homologous PV was always ≥ 4-fold higher than that of the heterologous and negative samples, which always displayed undetectable NI responses. The N1 and N2 PV-ELLA showed a strong linear trend between dilutions of the reference sample vs the respective GMT in the range of serum concentrations evaluated, with r2 coefficients always higher than 0.96 and 95% confidence intervals of the slope within the acceptability range, suggesting excellent linearity of the assay. The Geometric Mean Titers (GMTs) yielded in these linearity experiments show a maximum deviation of ± 1 titer step dilution (i.e. ≤ 2-fold difference in titers) when (i) collected within the same day or in different days, and when (ii) compared to those obtained in a pre-validation testing, suggesting precision and accuracy of the PV-ELLA. None of the variations applied to three critical steps of the assay produced a significant impact on NI titers, indicating that the ELLA performed with H11N1ACal/09 and H11N2HK/14 PV is adequately robust. In summary, the PV-ELLA performed with either N1- and N2-bearing PV has met all the acceptability criteria pre-defined for demonstration of specificity, linearity, precision, accuracy and robustness of the analytical method, confirming that the assay is suitable to measure NA antibody response in the experimental conditions evaluated. These results support the use of lentiviral pseudotypes as an attractive alternative source of NA in the ELLA assay and provide the means to standardize and validate the methodology in laboratories that do not have access to suitable reassortant viruses.

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3

Mahoney, Catherine H. "Studies using pseudotyped retroviral vectors". Thesis, University of Glasgow, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312721.

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4

Grzybowski, Brad. "A pseudotyped viral vector : hPIV3-HIV-1". Thesis, Georgia Institute of Technology, 2003. http://hdl.handle.net/1853/20932.

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5

Thomas, Joan Helen. "Studies in gene transfer using pseudotyped lentiviral vector systems". Thesis, University of Cambridge, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.621818.

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6

Gaiano, Nicholas R. (Nicholas Roger). "Insertional mutagenesis in zebrafish using a pseudotyped retroviral vector". Thesis, Massachusetts Institute of Technology, 1997. http://hdl.handle.net/1721.1/43311.

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Peng, Yue. "A novel vaccine strategy : replication-defective pseudotyped SIVs expressing IFN-[gamma] /". For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2005. http://uclibs.org/PID/11984.

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Thesis (Ph. D.)--University of California, Davis, 2005.
Degree granted in Comparative Pathology. On title page "[gamma]" appears as lower-case Greek letter. Also available via the World Wide Web. (Restricted to UC campuses)

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8

Kinsley, Rebecca. "Development of lentiviral pseudotypes for surveillance studies on animal influenza viruses". Thesis, University of Kent, 2017. https://kar.kent.ac.uk/66297/.

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Pseudotyped viruses (PVs) provide a safe, flexible platform for fundamental virological studies and antibody screening assays. Generation of influenza PVs involves co-transfection of producer cells with plasmids encoding the necessary viral components. The pseudotype virus neutralisation assay (PVNA) is a sensitive technique to measure protective antibody responses which cause neutralisation of virus particles. Many traditional methodologies, e.g. Haemagglutination Inhibition (HI) and Single Radial Haemolysis (SRH), detect only surface glycoprotein binding antibodies whereas the PVNA quantifies infectivity-neutralising responses. Haemagglutinin (HA) and neuraminidase (NA) are the two major surface glycoproteins embedded within the membrane of an influenza virion. HA is responsible for virion attachment and entry into a host cell and NA is essential for viral egress and thus spread of infection. Two enzymes crucial for the infectivity of influenza viruses are; HA-cleaving cellular proteases and the NA itself. Optimisation of both enzymes in PV production is necessary to increase the titre of PVs. Producing high titre PVs is important as this permits minimal quantities to be used in PVNAs, and repeat experiments can be carried out using the same batch of virus, minimising intra-study variability. Optimising PVs and employing them in a novel situation, such as an equine influenza vaccine efficacy trial, has been carried out with promising results. We have demonstrated that data obtained from the PVNA correlates well with the traditional SRH assay and consequently there is the potential for more widespread adoption in research and commercial settings. Furthermore, PVs have been manipulated to assess how single amino acid changes with equine influenza virus can affect the neutralisation efficacy of sera generated by vaccination. Novel PVs, such as those derived from canine and phocine (seal) influenza strains have also been produced and provide a new platform for sero-surveillance of these viruses particularly in the case of wild or feral animals, due to issues with obtaining samples from wild animals during acute infection. Overall, PVs have been demonstrated as useful and readily-manipulated tools for studying antibody responses against equine, canine and phocine influenza viruses.

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9

Carnell, George William. "Development of hybrid haemagglutinin pseudotyped lentiviruses to assess heterosubtypic immunity to influenza". Thesis, University of Kent, 2017. https://kar.kent.ac.uk/66363/.

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The influenza virus still causes hundreds of thousands of deaths globally, on top of morbidity and associated economic burden. We are currently at the height of efforts surrounding the development and employment of 'universal' vaccines against this virus, with clinical trials commencing on the most promising candidates. Despite this, the influenza virus poses more of a threat to human life than it ever has previously, with multiple subtypes of pandemic potential circulating around the globe. The key to current efforts lies in the priming of the immune system towards generating long lasting defences against conserved epitopes and conferring heterosubtypic immunity against the surface glycoprotein haemagglutinin. While vaccine strategies have expanded rapidly over recent years with the advent of 'headless' constructs as well as those derived from consensus, mosaic or chimeric sequences, the serological techniques to test how effective these vaccines are, have advanced less rapidly. Classical serological assays have been shown to be ineffective at detecting the antibodies which modern 'universal' vaccines strife to elicit, replaced by ELISA based approaches combined with mouse models measuring in vivo protection. In this thesis, an alternative method for the detection of heterosubtypic antibodies is used in depth across multiple platforms. Influenza pseudotypes have been employed using chimeric haemagglutinin constructs in a comprehensive project aimed at dissecting head and stalk directed antibodies present in human serum. Characterised broadly neutralising monoclonal antibodies have been tested on panels of influenza pseudotypes including divergent bat influenza viruses which hitherto have not been encountered in humans. A further aspect of influenza immunity has been covered in the detection of anti neuraminidase antibodies which have an important role to play in influenza heterosubtypic immunity. Finally, influenza pseudotypes bearing the glycoproteins from the less studied influenza B virus have been assayed in a large scale project aimed at correlating pseudotype assays with classical approaches.

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10

Amsterdam, Adam (Adam Henry) 1967. "Use of a pseudotyped retoviral vector to accomplish insertional mutagenesis in zebrafish". Thesis, Massachusetts Institute of Technology, 1998. http://hdl.handle.net/1721.1/50024.

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Libros sobre el tema "Pseudotype"

1

Pseudotyped Viruses. Springer, 2023.

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Capítulos de libros sobre el tema "Pseudotype"

1

Mi, Dan, Jiaxin Hu y Zhaohui Qian. "A Lentiviral Pseudotype System to Characterize SARS-CoV-2 Glycoprotein". En Methods in Molecular Biology, 187–99. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2895-9_16.

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2

Nehring, Dirk, Ralf Pörtner y Peter Czermak. "Production of Retroviral Pseudotype Vectors in Fixed Bed Reactors for Use in Gene Therapy". En Cells and Culture, 625–28. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3419-9_107.

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3

Zavada, J. "VSV(BLV) Pseudotypes". En Enzootic Bovine Leukosis and Bovine Leukemia Virus, 219–26. Boston, MA: Springer US, 1987. http://dx.doi.org/10.1007/978-1-4613-2341-9_17.

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4

Kuo, Tzu-Hsing y Jessica L. Whited. "Pseudotyped Retroviruses for Infecting Axolotl". En Methods in Molecular Biology, 127–40. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2495-0_10.

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5

Ji, Zhicheng y Hongkai Ji. "Pseudotime Reconstruction Using TSCAN". En Methods in Molecular Biology, 115–24. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9057-3_8.

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6

Landau, Nathaniel R., Kathleen A. Page y Dan R. Littman. "Envelope Pseudotypes of HIV and HTLV". En Advances in Molecular Biology and Targeted Treatment for AIDS, 225–33. Boston, MA: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4684-5928-9_20.

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7

Sinn, Patrick L., Jeremy E. Coffin, Natarajan Ayithan, Kathleen H. Holt y Wendy Maury. "Lentiviral Vectors Pseudotyped with Filoviral Glycoproteins". En Ebolaviruses, 65–78. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7116-9_5.

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8

Yu, Hong y Young Jik Kwon. "Preparation and Quantification of Pseudotyped Retroviral Vector". En Methods in Molecular Biology, 1–16. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-237-3_1.

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9

Hu, Shuang, Mingjie Li y Ramesh Akkina. "Generation of High-Titer Pseudotyped Lentiviral Vectors". En Methods in Molecular Biology, 125–34. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9065-8_7.

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10

Rupil, Lucía Lara, Marianela del Carmen Serradell y Hugo Daniel Luján. "Production of Based on Virus-Like Particles Pseudotyped with". En Vaccine Design, 503–37. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1884-4_26.

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Actas de conferencias sobre el tema "Pseudotype"

1

Taveira, Elisa Borges, Marco Fidel Guevara-Vega, Igor Andrade Santos, Douglas Carvalho Caixeta, Victoria Riquena Grosche, Thulio Marquez Cunha, Murillo Guimarães Carneiro, Ana Carolina Gomes Jardim y Robinson Sabino-Silva. "SARS-CoV-2 structures detection in artificial saliva using ATR-FTIR associated with Linear Discriminant Analysis". En Latin America Optics and Photonics Conference. Washington, D.C.: Optica Publishing Group, 2022. http://dx.doi.org/10.1364/laop.2022.tu1c.8.

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Resumen

Here, we used ATR-FTIR platform supported by artificial intelligence algorithms to identify unique infrared vibrational modes of a pseudotyped human immunodeficiency virus type-1 (HIV-1) coupled to Spike (S) protein of SARS-CoV-2 (HIV/NanoLuc-SARS-CoV-2 pseudotype virus).

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2

Aqila, Tasmia y Ananda Mohan Mondal. "Pseudotime Based Analysis of Cancer Dynamics". En BCB '19: 10th ACM International Conference on Bioinformatics, Computational Biology and Health Informatics. New York, NY, USA: ACM, 2019. http://dx.doi.org/10.1145/3307339.3343253.

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3

Aminian, K., S. Ameri, W. E. Abbitt y L. E. Cunningham. "Polynomial Approximations for Gas Pseudopressure and Pseudotime". En SPE Eastern Regional Meeting. Society of Petroleum Engineers, 1991. http://dx.doi.org/10.2118/23439-ms.

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4

Aqila, Tasmia, Abdullah Al Mamun y Ananda Mohan Mondal. "Pseudotime Based Discovery of Breast Cancer Heterogeneity". En 2019 IEEE International Conference on Bioinformatics and Biomedicine (BIBM). IEEE, 2019. http://dx.doi.org/10.1109/bibm47256.2019.8983300.

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5

Tabatabaie, S. Hamed, Louis Mattar y Mehran Pooladi-Darvish. "Pseudotime Calculation in Low Permeability Gas Reservoirs". En SPE Unconventional Resources Conference Canada. Society of Petroleum Engineers, 2013. http://dx.doi.org/10.2118/167185-ms.

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6

E. Plessix, R., P. Milcik, C. Corcoran, H. Kuehl y K. Matson. "Full Waveform Inversion with a Pseudotime Approach". En 74th EAGE Conference and Exhibition incorporating EUROPEC 2012. Netherlands: EAGE Publications BV, 2012. http://dx.doi.org/10.3997/2214-4609.20148713.

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7

Alton, EWFW, AC Boyd, JC Davies, DR Gill, U. Griesenbach, TE Harman, SC Hyde y G. McLachlan. "S68 Towards a first-in-human trial with a pseudotyped lentivirus". En British Thoracic Society Winter Meeting, Wednesday 17 to Friday 19 February 2021, Programme and Abstracts. BMJ Publishing Group Ltd and British Thoracic Society, 2021. http://dx.doi.org/10.1136/thorax-2020-btsabstracts.73.

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8

Aanonsen, S. "Application of Pseudotime To Estimate Average Reservoir Pressure". En SPE Annual Technical Conference and Exhibition. Society of Petroleum Engineers, 1985. http://dx.doi.org/10.2118/14256-ms.

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9

Wu, Yunheng y Meixue Li. "Study on fatty liver based on Pseudotime analysis". En ICBIP 2022: 2022 7th International Conference on Biomedical Signal and Image Processing. New York, NY, USA: ACM, 2022. http://dx.doi.org/10.1145/3563737.3563744.

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10

Smith, Blake, Stephanie Dougan y Michael Birnbaum. "1232 Engineering a pseudotyped lentiviral platform to enable lineage-specific transduction of immune cells". En SITC 37th Annual Meeting (SITC 2022) Abstracts. BMJ Publishing Group Ltd, 2022. http://dx.doi.org/10.1136/jitc-2022-sitc2022.1232.

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