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Literatura académica sobre el tema "Semen"

Autor: Grafiati
Publicado: 4 de junio de 2021
Última modificación: 9 de junio de 2025

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Artículos de revistas sobre el tema "Semen"

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E., O. Ewuola, and E. Akinyemi D. "Semen Characteristics of Rabbit Bucks Orally Administered Exogenous L-Selenomethionine." Annual Research & Review in Biology 13, no. 3 (June 9, 2017): 1–8. https://doi.org/10.9734/ARRB/2017/19196.

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An experiment was carried out to evaluate the semen quality of rabbits administered varied levels of exogenous L-Selenomethionine (L-SeMet). Twenty four male rabbits (10 months old) were randomly allotted to four treatments in a completely randomised design. Treatment 1 (control) was without exogenous L-SeMet, while treatments 2, 3 and 4 were administered 0.2 mgkg<sup>-1</sup>, 0.3 mgkg<sup>-1</sup> and 0.4 mgkg<sup>-1</sup> oral supplementation of L-SeMet, respectively at 48hours interval for 6 weeks. Semen samples were collected at day 21 and day 42 using artificial vagina from the bucks and assessed for volume, mass activity, progressive motility, percentage dead and live sperm cells and sperm concentration. Sperm progressive motility and sperm concentration increased significantly (P&lt;0.05) as the concentration of L-SeMet increases at day 21. However, percentage dead sperm cells significantly (P&lt;0.05) reduced with increase in the level of L-SeMet supplementation. At day 42, it was also observed that mass activity, sperm progressive motility and sperm concentration were significantly (P&lt;0.05) highest in rabbits administered 0.4 mgkg<sup>-1</sup> L-SeMet. However, semen volume was not significantly different among the treatments on both days of assessment. This suggested that exogenous supplementation of L-SeMet up to 0.4 mgkg<sup>-1</sup> improved semen attributes and sperm cells formation of the rabbit bucks.
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Alkindi. "Monitoring Kepuasan Pelanggan Terhadap Marketing Mix PT XYZ Menggunakan Diagram Pengendali T2 Hotelling dan Generalized Varians." Syntax Literate ; Jurnal Ilmiah Indonesia 9, no. 12 (December 27, 2024): 7734–52. https://doi.org/10.36418/syntax-literate.v9i12.52400.

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Industri di Indonesia terus berkembang dan semakin berkreasi, tidak terkecuali pada industri persemenan. Jika dibandingkan tahun 2009, jumlah produsen semen saat ini bertambah menjadi dua kali lipat. Kenaikan kapasitas terpasang dan bertambahnya produsen semen tidak diimbangi oleh pertumbuhan permintaan semen yang mengakibatkan pada menurunnya utilitas pabrik-pabrik produsen. Kondisi di tahun 2024 merupakan kondisi oversupply dimana terdapat 18 produsen semen dengan kapasitas terpasang pabrik sebesar 122 juta ton/tahun sedangkan permintaan semen sebesar 65 juta ton menyebabkan utilitas pabrik produsen semen menjadi 53,1% yang tergolong rendah. Tren penurunan utilitas ini merupakan efek lanjutan akibat banyaknya produsen semen baru yang terdaftar sejak tahun 2015. Persaingan yang ketat di industri semen membuat produsen semen diharuskan menjaga kualitas dan performa di segmen marketing. Salah satu konsep marketing yang diterapkan di industri semen adalah marketing mix 7P yaitu strategi pemasaran dari segi product, price, promotion, place, people, process, dan physical evidence. Ketujuh elemen tersebut membuat konsep marketing menjadi lebih kompleks karena juga melibatkan aspek di luar produk yang dipasarkan. Penelitian ini akan melakukan analisa dan pengendalian marketing mix 7P terhadap kepuasan konsumen. Identifikasi dan penerapan marketing mix 7P menggunakan diagram pengendali T2 Hotelling dan Generalized Varians. Berdasarkan diagram pengendali Generalized Varians, kepuasan pelanggan tidak terkendali terhadap varians di tahun 2020. Faktor yang menyebabkan terjadinya keadaan yang luar biasa adalah place dan process. Berdasarkan evaluasi dan monitoring diagram pengendali, diketahui bahwa masalah utama pada kategori place adalah semen sulit ditemukan atau stok semen terbatas sedangkan masalah utama pada kategori process adalah waktu pengiriman sering terlambat. Pada Diagram Ishikawa dijelaskan bahwa penyebab tersebut mayoritas terjadi karena adanya pandemi COVID-19 mengakibatkan Kunjungan tenaga penjualan ke toko berkurang dan semakin jarang yang diakibatkan tenaga penjualan belum berpengalaman menghadapi situasi khusus dan sedikitnya toko bangunan yang masih buka.
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Budai, Csilla, István Egerszegi, József Rátky, and András Kovács. "Storage of ram semen in gelatin supplemented extender." Acta Agraria Debreceniensis, no. 48 (July 31, 2012): 7–10. http://dx.doi.org/10.34101/actaagrar/48/2444.

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The aim of our study was to examine how different gelatin concentrations affect ram semens viability in liquid storage at 5 oC for five days. Our hypothesis was if we add gelatin to the semen extender, than the viability of ram semen will be better in the extenders containing gelatin, than the control. We used two different semen extenders:1.5% UHT milk and 1.5% UHT milk + 5% egg yolk. We added 0; 0.5; 1.0; 1.5; 2.0% Dr. Oetker gelatin to the semen extenders. We stored the semen for five days at 5 oC and in every 24 hour we made sampling.We stained the smears with Kovács-Foote staining and evaluated them with light-microscope. We categorized the cells in five groups like: live and intact cells, live cells with injured acrosome, dead cells, live head with dead tail and live tail with dead head. We used one-way analysis of variance (ANOVA) to assign how gelatin concentration affects the number of the categorized cells. On the fifth day, the viability was the best in the following semen extenders: 1.5% fat UHT milk + 1.0% gelatin and 1.5% fat UHT milk + 1.5% gelatin, but it was not significant (p&gt;0.05).
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Utami, Liona Harmoni, Chundakus Habsya, and Rima Sri Agustin. "Pengaruh Fly Ash sebagai Pengganti Sebagian Semen terhadap Berat Isi dan Kuat Tekan Segmen Kolom Praktis Modular Ramah Lingkungan." Indonesian Journal Of Civil Engineering Education 9, no. 1 (July 31, 2023): 14. http://dx.doi.org/10.20961/ijcee.v9i1.66607.

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&lt;p&gt;Limbah &lt;em&gt;fly ash&lt;/em&gt; terus meningkat sebanding dengan meningkatnya produksi semen dan kebutuhan manusia dalam penggunaan listrik. &lt;em&gt;Fly ash &lt;/em&gt;tergolong dalam limbah B3 berdampak negatif bagi kesehatan dan lingkungan. Penggunaan limbah &lt;em&gt;fly ash&lt;/em&gt; pada beton dapat meningkatkan kuat tekan beton, sehingga menjadi salah satu solusi mengurangi dampak negatif limbah &lt;em&gt;fly ash&lt;/em&gt;. &lt;em&gt;Fly ash &lt;/em&gt;akan digunakan sebagai pengganti sebagian semen dengan 4 variasi sempel segmen kolom praktis (SKPM) berukuran 150 mm x 150 mm x 150 mm dengan lubang tengah berukuran 80 mm x 80 mm x 150 mm sebanyak 32 buah untuk pengujian berat isi dan kuat tekan SKPM. Tujuan penelitian ini untuk mengkaji: (1) Pengaruh &lt;em&gt;fly ash&lt;/em&gt; sebagai pengganti sebagian semen terhadap berat isi Segmen Kolom Praktis Modular (SKPM). (2) Pengaruh &lt;em&gt;fly ash&lt;/em&gt; sebagai pengganti sebagian semen terhadap semen kuat tekan SKPM. (3) Hubungan berat isi dan kuat tekan SKPM. (4) Pengaruh &lt;em&gt;fly ash&lt;/em&gt; sebagai pengganti sebagian semen terhadap kriteria beton ramah lingkungan. Penelitian metode kuantitatif eksperimental dilakukan di laboratorium. SKPM menggunakan perbandingan semen dan pasir sebesar 1:7 dengan nilai faktor air binder (fab) 0,48. Pengujian berat isi mengacu SNI 1973:2008 dan pengujian kuat tekan mengacu pada SNI 03-0349-1989. Perawatan beton dilakukan selama 28 hari dari waktu pembuatan sampai waktu pengujian. Hasil penelitian ini adalah: (1) Adanya pengaruh &lt;em&gt;fly ash&lt;/em&gt; sebagai pengganti sebagian semen terhadap semen mengakibatkan kenaikan berat isi SKPM dengan nilai maksimal sebesar 2013,261 kg/m³ pada variasi &lt;em&gt;fly ash&lt;/em&gt; 30%. (2) Adanya pengaruh &lt;em&gt;fly ash&lt;/em&gt; sebagai pengganti sebagian semen mengakibatkan terjadi kenaikan kuat tekan SKPM dengan nilai maksimal sebesar 73,561 kgf/cm² pada variasi &lt;em&gt;fly ash&lt;/em&gt; 20%. (3) Berat isi SKPM memenuhi standar SNI 1973:2008 dan kuat tekan SKPM memenuhi standar 03-0349-1989 mutu I dengan nilai maksimal variasi 20% &lt;em&gt;fly ash&lt;/em&gt;. (4) Adanya pengaruh &lt;em&gt;fly ash&lt;/em&gt; sebagai pengganti sebagian semen memiliki ciri-ciri kriteria beton ramah lingkungan sesuai dengan kategori penilaian Material &lt;em&gt;Resource and Cycle&lt;/em&gt; pada perangkat penilaian &lt;em&gt;Greenship Rating Tools&lt;/em&gt; versi 1.0 dengan &lt;em&gt;recycle&lt;/em&gt; limbah dan membuat material SKPM prapabrikasi.&lt;/p&gt;
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Akbar Irawan and Mohammad Azhar. "ANALISIS PENGGUNAAN SLAG UNTUK MEREDUKSI SEMEN PADA CAMPURAN BETON." Jurnal Gradasi Teknik Sipil 6, no. 2 (December 30, 2022): 142–49. http://dx.doi.org/10.31961/gradasi.v6i2.1471.

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Penelitian penggunaan slag semen pada campuran beton sebagai cementious telah dimulai sejak tahun 1774. Saat ini di negara-negara maju slag semen telah dimanfaatkan pada setiap campuran beton, bahkan persentasenya mencapai 30% dari cementious, hal ini dapat dilihat dari produksi slag semen yang terus meningkat dari tahun ke tahun. Pembuatan beton dengan menggunakan slag sement sebagai campuran agregat halus untuk subtitusi terhadap semen yang selanjutnya dilakukan pengujian terhadap benda uji beton untuk mengetahui seberapa besar pengaruhnya terhadap nilai kuat tekan pada beton, serta bertujuan juga untuk mendapatkan campuran bahan beton dengan biaya yang lebih efisien dibandingkan beton non-slag. Kuat tekan Beton akan mengidentifikasi mutu dari sebuah konstruksi, semakin tinggi kekuatan struktur yang direncanakan , maka semakin tinggi pula mutu beton yang harus di hasilkan. Beberapa faktor yang mempengaruhi kuat tekan beton, yaitu faktor air semen (w/c), jumlah air, jumlah dan kualitas semen, kualitas agregat, umur beton, serta perawatan beton. Apabila w/c tidak sesuai dengan beton yang direncanakan maka beton akan mengalami perubahan kekuatan. Hubungan antara kuat tekan beton dengan nilai w/c berbanding terbalik, kuat tekan akan menjadi tinggi apabila angka w/c kecil dan sebaliknya kuat tekan akan rendah apabila w/c makin besar. Hasil penelitian ini menunjukan bahwa perbandingan komposisi untuk memperoleh mutu yang sama didapatkan hasil pada beton slag ada penambahan air 7 liter/m³ terhadap non-slag, dan mengurangi sementious sebesar 2 kg/m³, serta slag semen memberikan kontribusi terhadap kuat tekan lebih besar dari pada beton non-slag sebesar 4,3%, akan tetapi dalam mencapai hal itu, slag semen membutuhkan air yang lebih banyak, sedangkan dari segi biaya, dibandingkan beton non-slag, slag semen dapat menekan biaya sebesar Rp 15.354,00/m3.
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Dasrul, Dasrul, Sri Wahyuni, Sugito Sugito, Abdulah Hamzah, Zulzya Zaini, Abdul Haris, and Gholib Gholib. "Correlation Between Testosterone Concentrations with Scrotal Circumference, and Semen Characteristics in Aceh Bulls." E3S Web of Conferences 151 (2020): 01015. http://dx.doi.org/10.1051/e3sconf/202015101015.

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This study was conducted to examine testosterone concentrations its relationship with the scrotal circumference and physical characteristics of semen in aceh bulls. Semen samples were collected weekly from jugular vein of three aceh bulls aged 4-5 years old for 10 weeks. Testosterone concentration was measured by enzyme-linked immunosorbent assay (ELISA) method. Semens were collected by using artificial vagina and evaluated for physical characteristics namely ejaculatory volume, pH, and sperm motility, concentration, and abnormalities. Data were analyzed using correlation-regresion test. Testosterone concentrations showed a positive correlation with scrotal circumference (r = 0.799), number of sperm (r = 0.703), sperm motility (r = 0.857) and sperm abnormalities (r = -0,877). No correlation, however, was found between testosterone concentrations with semen volume (r = 0.038) and pH (r = 0.418). It can be concluded that testosterone concentrations correlated positively with scrotal circumference, numbers of sperm, sperm of motility and sperm of abnormality.
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La Vignera, Sandro, Rosita A. Condorelli, Enzo Vicari, Dario Tumino, Giuseppe Morgia, Vincenzo Favilla, Sebastiano Cimino, and Aldo E. Calogero. "Markers of semen inflammation: supplementary semen analysis?" Journal of Reproductive Immunology 100, no. 1 (November 2013): 2–10. http://dx.doi.org/10.1016/j.jri.2013.05.001.

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Lee, Seungho, Youngsik Kim, Junghoon Kim, Guemsan Lee, Goya Choi, and Youngsung Ju. "Identification of Armeniacae Semen and Persicae Semen." Korea Journal of Herbology 29, no. 4 (July 30, 2014): 61–67. http://dx.doi.org/10.6116/kjh.2014.29.4.61.

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Riskiah, Delian Aupanisa, and Safaruddin Safaruddin. "PROSES PRODUKSI SEMEN PORTLANT PT. SEMEN BATURAJA." Jurnal Multidisipliner Bharasumba 1, no. 04 (October 10, 2022): 430–44. http://dx.doi.org/10.62668/bharasumba.v1i04.297.

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Proses pembuatan semen yang dilakukan pada PT. Semen Baturaja (Persero) Tbk ini menggunakan proses kering (Dry process). Proses produksi ini dimulai dari penyediaan bahan mentah, penggilingan bahan mentah, pembakaran, pendinginan klinker, penggilingan klinker, dan pengantongan semen.pada pt semen baturaja terdapat tambang batu kapur ( lime stone) dan tanah liat (clay). Kemudian penggilingan batu kapur dengan menggunakan dobble hammer, dan untuk tanah liat menggunakan dobble roller hamer. Yang terjadi pada alat raw mill yaitu pencampuran bahan, penghancuran, pengayakan, dan pemanasan. pada proses kiln membentuk klinker yaitu salah satu bahan pembuat semen.hasil dari proses klinkerinasisi itu sendiri tidak hanya di salurkan langsung pada poroses cemen mill, tetapi klinker juga di salurkan ke baprik pt semen batubara di unit cemen mill yg ada di palembang. Jadi proses kilinkerinasi sendiri hanya di lakukan di pt semen baturaja yang berad di batu raja. proses produksi ini menggunakan bahan baku utama yaitu batu kapur (lime stone) dan juga tananah liat (clay). Pada produksi ini juga menggunakan bahan tambahan yaitu pasir besi dan pasir silika. pada cemen mill menggunakan pozolan, klinker, dan gypsum. PT semen baturaja memproduksi 2 tipe semen yaitu semen OPC dan juga semen PCC, yang dibedakan oleh komposisi klinker yg ada pada semen sesuai dengan kebutuhan semen itu sendiri.PT. Semen Baturaja (Persero) Tbk mulai beroperasi secara komersil mulai pada tanggal 1 Juni 1981 dengan kapasitas produksi 450.000 ton klinker per tahun dan untuk meningkatkan kapasitas produksi untuk memenuhi kebutuhan semen maka dilaksanakan optimalisasi produksi klinker secara berkala mulai dari optimalisasi proyek terak-I sampai optimalisasi proyek terak –II (OPT-I sampai OPT-II).
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Kim, Young-Sik, and Young-Sung Ju. "Identification of Morphological Appearance of Minute Seed Herbs Using Stereoscope (Report II) - Alli Tuberosi Semen✳Alli Fistulosi Semen, Malvae Semen✳Abutili Semen, Plantaginis Semen✳Schizonepetae Semen." Korea Journal of Herbology 31, no. 4 (July 30, 2016): 61–69. http://dx.doi.org/10.6116/kjh.2016.31.4.61.

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Tesis sobre el tema "Semen"

1

Badenoch, D. F. "Semen analysis and fertility." Thesis, University of Oxford, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.375208.

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Carson, Claire Elizabeth. "Risk factors for poor semen quality : a study of men undergoing semen analysis." Thesis, London School of Hygiene and Tropical Medicine (University of London), 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.424325.

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Vilakazi, David Mxolisi. "Factors affecting the quality of semen of AI dairy bulls in South Africa." Pretoria : [s.n.], 2005. http://upetd.up.ac.za/thesis/available/etd-09022005-150724.

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Farrás, Marcel Cavalcanti [UNESP]. "Avaliação da fragmentação do DNA espermático de sêmen refrigerado de garanhões." Universidade Estadual Paulista (UNESP), 2012. http://hdl.handle.net/11449/98144.

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Made available in DSpace on 2014-06-11T19:29:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-01-26Bitstream added on 2014-06-13T18:58:50Z : No. of bitstreams: 1 farras_mc_me_botfmvz.pdf: 544796 bytes, checksum: 513d0af00d97f12c80a9d388b7fd8f78 (MD5)<br>Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)<br>Nas últimas décadas, muitos estudos têm sido realizados nas diferentes espécies com o intuito de se determinar os fatores envolvidos no processo da fragmentação do DNA espermático. A biologia molecular voltada à área da reprodução tem resultado em inúmeras técnicas para a avaliação da qualidade da cromatina e determinação da fragmentação do DNA espermático. Em trabalhos recentes, um novo teste denominado Halomax® tem se mostrado tão eficiente quanto a outros tradicionais para a avaliação da fragmentação do DNA espermático, apresentando como vantagens a sua praticidade e o fato não requerer o uso de equipamentos de alto custo. O presente experimento teve por objetivo comparar as raças Mangalarga Marchador (MM) e Quarto de Milha (QM) quanto à resistência à refrigeração, bem como utilizar o Teste Dispersão de Cromatina Espermática denominado Halomax® para avaliar a fragmentação da cromatina espermática do sêmen refrigerado dos garanhões dessas raças em duas temperaturas de armazenamento de sêmen refrigerado (5°C e 15°C). Os resultados deste trabalho demonstraram que não houve diferença entre as temperaturas de armazenamento (5°C e 15°C), mostran do que ambas são eficientes na manutenção da viabilidade espermática pelo período de 24 horas para ambas as raças estudadas. Além disso, foi observada uma característica espermática superior dos garanhões da raça Quarto de Milha quando comparada aos da raça Mangalarga Marchador, tanto nos parâmetros de velocidade espermática, avaliado pelo CASA, quanto com relação à fragmentação do DNA espermático, revelando uma maior sensibilidade dos animais da raça Mangalarga ao processo de refrigeração do sêmen<br>Many studies have been conducted in different species in order to determine the factors involved in the process of sperm DNA fragmentation. Molecular biology studies focused on the reproductive area has resulted in several techniques for assessing the quality of chromatin and sperm DNA fragmentation. Recently, a new test called HALOMAX ® has been shown to be as efficient as other traditional assessment of sperm DNA fragmentation, having the advantage of practicality and not require the use of expensive equipment. The aim of this study was to compare breeds Mangalarga and Quarter Horses for cooling resistance and use the Sperm Chromatin Dispersion Test called HALOMAX ® to evaluate the sperm chromatin fragmentation using two cooled semen storage systems (5°C and 15°C). Our results showed no difference between storage systems (5°C and 15°C), showing that both are effective in maintaining sperm viability for a period of 24 hours for both breeds studied. In addition, the Quarter Horse shown superiority in semen quality parameters assessed by CASA and sperm DNA fragmentation when compared with the Mangalarga, revealing higher sensitivity of the Mangalarga stallions for cooling semen
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Falleiros, Marcel Barbosa [UNESP]. "Avaliação comparativa do sêmen ovino refrigerado nos meios Glicina-Gema-Leite, Glicina-Gema purificada-Leite e Glicina-extrato de lipoproteínas de baxa densidade-Leite." Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/98149.

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Made available in DSpace on 2014-06-11T19:29:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-01-29Bitstream added on 2014-06-13T19:17:39Z : No. of bitstreams: 1 falleiros_mb_me_botfmvz.pdf: 753532 bytes, checksum: 09df2201bbfb5625659993b6522a5251 (MD5)<br>Universidade Estadual Paulista (UNESP)<br>Objetivou-se estudar os efeitos do extrato de lipoproteínas de baixa densidade sobre o sêmen ovino durante a refrigeração. Vinte amostras de 5 carneiros foram refrigeradas por 24 e 48 horas na geladeira para refrigeração Minitube, nos meios diluentes Glicina Gema Leite, Glicina Gema purificada Leite e Glicina Extrato Leite e submetidas a teste de exaustão (37ºC/240 minutos) sendo avaliados in vitro por meio das análises da cinética espermática computadorizada, da morfologia e da integridade da membrana plasmáticas. Após 24 e 48 horas de refrigeração, os meios Glicina Gema purificada Leite e Glicina Extrato Leite apresentaram resultados superiores ao meio Glicina Gema Leite, após o teste de exaustão, para o parâmetro de integridade de membrana plasmática. Para a integridade de acrossomo o meio Glicina Gema purificada Leite foi superior (P<0,05) em relação ao meio Glicina Gema Leite durante o teste de exaustão. Nos demais parâmetros estudados de cinética espermática e morfologia (cauda dobrada), não houveram diferenças significativas (P>0,05) entre os meios. Entre os momentos, houve diferença significativa (P<0,05) em todos os meios durante o teste de exaustão<br>The objective was to study the effects of extract of low density lipoproteins on ovine semen during cooling. Twenty samples of five sheep were chilled for 24 and 48 hours in the refrigerator for cooling Minitube, in extenders Glycine Yolk Milk, Glycine purified Yolk Milk and Glycine Extract Milk and tested to exhaustion (37 ° C/240 min) were evaluated in vitro by means of computerized analysis of sperm kinetics, morphology and plasma membrane integrity. After 24 and 48 hours of refrigeration, the extenders Glycine purified Yolk Milk and Glycine Extract Milk showed better results than extender Glycine Yolk Milk, after the exhaustion test, for the parameter of membrane integrity. For the integrity of the acrosome through Glycine purified Yolk Milk was higher (P <0.05) than Glycine Yolk Milk during the exhaustion test. In other parameters of sperm kinetics and morphology, there were no significant differences (P> 0.05) among extenders. Between times, significant difference (P <0.05) in all extenders during the exhaustion test
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Arman, Chairussyuhur. "Use of compounds of epididymal and plant origin in maintaining viability of ram spermatozoa." Title page, contents and summary only, 1996. http://web4.library.adelaide.edu.au/theses/09PH/09pha727.pdf.

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Bibliography: leaves 267-292. This study aimed at finding better ways of storing ram semen at refrigerator or room temperature with particular reference to ingredients readily available in Indonesia, namely coconut extract and quail egg yolk. Coconut extract showed consistent advantages with regard to sperm motility and quail egg yolk was as effective as hen egg yolk. Investigations were extended to examine storage for subnormal semen such as would be produced during periods of heat stress. Motility was assessed visually and using a Hamilton Thorn semen evaluation apparatus.
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Dirscherl, Friederike Karin Maria [Verfasser]. "Effects of semen collection practices on the bacterial load of stallion semen / Friederike Karin Maria Dirscherl." Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2011. http://d-nb.info/1013294599/34.

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Gacem, Sabrina. "Computerised analysis of semen in equids." Doctoral thesis, Universitat Autònoma de Barcelona, 2021. http://hdl.handle.net/10803/672020.

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L’enfocament que es proposa en aquesta tesi es basa en el fet que hi ha un buit d’informació sobre l’optimització de l’examen de la solidesa reproductiva juntament amb els protocols d’anàlisi de semen en els equids, tal com es va veure al capítol anterior. A l’ase, no es van definir protocols específics per a l’anàlisi de semen, només per a mostres de sementals on hi ha un acord general sobre el protocol a utilitzar. Quan es comprova el tracte reproductor, s’utilitza una ecografia en mode B com a mètode de diagnòstic fàcil i no invasiu. D’altra banda, es pot utilitzar una ecografia Doppler polsada per estudiar la perfusió testicular de sang. Els índexs de Doppler pulsat de l’artèria testicular i les mides ASG van mostrar una correlació positiva amb la qualitat del semen en sementals i diferents espècies. Malauradament, no es van fer estudis d’aquest tipus en ruc, cosa que constitueix el primer objectiu de la tesi. No obstant això, quan es tracta d’anàlisi de semen, la tecnologia CASA representa el millor mètode objectiu que s’utilitza avui en dia. Permet obtenir dades quantitatives fiables, fins i tot si és necessari definir protocols que assegurin la consistència i l’aplicació universal dels resultats. Tanmateix, aquesta estandardització mai s’ha fet seguint un punt de vista integrador, que compon el segon objectiu general. Hi ha tres aspectes principals a tenir en compte a l’hora d’optimitzar les anàlisis automatitzades de semen mitjançant la tecnologia CASA-Mot, a saber, el tipus, la profunditat de la cambra de comptatge, la dilució i la velocitat de fotogrames d’adquisició d’imatges. Els avenços tecnològics van permetre ara l’adquisició d’alta freqüència de captura i, al mateix temps, el procés d’aquestes imatges en segons. A més, es va definir l’estructura de les subpoblacions d’espermatozoides en marcs més alts i la distribució SP entre les races dels sementals. El tercer objectiu era col·laborar en l’ús i la millora de la nova tècnica Trumorph®, per a l’anàlisi de morfologia de l’esperma que permet l’anàlisi d’espermatozoides immobilitzats vius.<br>El enfoque defendido en esta tesis se basa en el hecho de que existe un vacío de información con respecto a la optimización del examen de solidez reproductiva junto con los protocolos de análisis de semen en équidos, como se vio en el capítulo anterior. En burro, no se definieron protocolos específicos para el análisis de semen, solo para muestras de sementales donde existe un acuerdo general sobre el protocolo a utilizar. Cuando se comprueba el tracto reproductivo, la ecografía en modo B se utiliza como un método de diagnóstico fácil y no invasivo. Por otro lado, la ecografía Doppler pulsado se puede utilizar para estudiar la perfusión sanguínea testicular. Los índices de Doppler pulsado de la arteria testicular y los tamaños de ASG mostraron una correlación positiva con la calidad del semen en sementales y diferentes especies. Lamentablemente, estos estudios no se realizaron en burro, lo que constituye el primer objetivo de la tesis. Sin embargo, cuando se trata de análisis de semen, la tecnología CASA representa el mejor método objetivo utilizado en la actualidad. Permite obtener datos cuantitativos confiables, incluso si es necesario para definir protocolos asegurando la consistencia y aplicación universal de los resultados. Sin embargo, esta estandarización nunca se ha hecho siguiendo un punto de vista integrador, que compone el segundo objetivo general. Hay tres aspectos principales a considerar al optimizar los análisis de semen automatizados mediante la tecnología CASA-Mot, a saber, el tipo, la profundidad de la cámara de recuento, la dilución y la velocidad de cuadros de la adquisición de imágenes. Los avances en tecnología permitieron ahora la adquisición de alta frecuencia de captura y al mismo tiempo el proceso de estas imágenes en segundos. Además, se definió la estructura de las subpoblaciones de espermatozoides en cuadros más altos y la distribución de SP entre las razas de sementales. El tercer objetivo fue colaborar en el uso y la mejora de la nueva técnica Trumorph®, para el análisis de la morfología espermática que permite el análisis de espermatozoides inmovilizados vivos.<br>The approach advocated in this thesis is based on the fact that there is a gap of information regarding optimization of breeding soundness examination along with semen analysis protocols in equids as seen in the previous chapter. In donkey, no specific protocols for semen analysis were defined, only for stallion samples where there is a general agreement about the protocol to use. When the reproductive tract is checked, B-mode ultrasonography is used as a non-invasive and easy diagnostic method. On the other hand, pulsed Doppler ultrasonography can be used to study testicular blood perfusion. Pulsed-Doppler indices of testicular artery and ASG sizes showed a positive correlation with semen quality in stallion and different species. Unfortunately, no such studies were done in donkey, which makes up the first objective of the thesis. However, when it’s about semen analysis, CASA technology represents the best objective method used today. It permits to obtain reliable quantitative data, even if it is needed to define protocols assuring the consistency and universal application of the results. However, this standardization has never been done following an integrative point of view, which composes the second general objective. There are three main aspects to consider when optimizing automated semen analyses by CASA-Mot technology, namely the type, depth of the counting chamber, the dilution, and the frame rate of image acquisition. The advances in technology allowed now the acquisition of high capture frequency and at the same time the process of these images in seconds. Also, was defined sperm subpopulations structure at higher frames and SP distribution between stallions’ breeds. The third objective was to collaborate in the use and the improvement of the new Trumorph® technique, for sperm morphology analysis which permits the analysis of alive immobilized sperm.<br>Universitat Autònoma de Barcelona. Programa de Doctorat en Medicina i Sanitat Animals
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Mataveia, Gracinda Andre. "Effect of heterologous seminal plasma and semen extenders on motility of frozen-thawed ram sperm." Diss., Pretoria : [s.n.], 2006. http://upetd.up.ac.za/thesis/available/etd-05142008-123139/.

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Vogler, Cheryl Jean. "Effects of elevated testicular temperature on viability of cryopreserved semen and morphological characteristics of ejaculated spermatozoa." Thesis, Virginia Tech, 1990. http://hdl.handle.net/10919/42217.

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Two successive ejaculates were collected from six mature Holstein bulls at 3 d intervals for 7 wks. Elevated testicular temperature was induced by complete coverage of the scrotum with insulated material for 48 h. Viability (motility and acrosome integrity) and morphological characteristics of sperm before and after thermal insult were examined. For assessment of results, collection days were grouped: days -6, -3, 0 = Period 1 (d 0 = day of testis coverage after semen collection on that day), days 3, 6, 9 = Period 2 , days 12, 15...39 = Period 3. Semen was cryopreserved on each day of collection until morphological abnormalities of sperm increased to >50%. Semen viability before and after freezing was lower in Period 3 than in Period 1 (P≤.01). These differences coincided with increased abnormal morphology. No differences in viability were observed between Period 1 and Period 2 for unfrozen semen. Once frozen, spermatozoa ejaculated during Period 2 were significantly different from Period 1 for both viability measurements, but only after 3 h incubation at 37°C (P≤.01). Mean percent pre-insult abnormal sperm level was 19.6 ± 5.7 and sperm morphology in Period 1 (pre-insult) did not differ from that in Period 2. Morphological change was first noted in Period 3 on d 12 and 15 (47.5 ± 27.4 and 65.0 ± 27.0 % abnormal sperm, respectively). Abnormal sperm peaked on d 21 (83.2 ± 22.8 %). Although bulls varied in degree and time of response post-insult, all bulls exhibited the same sequence of appearance for specific abnormalities. The sequence and peak means for these abnormalities observed over all bulls were as follows: decapitated sperm, d 15 (33.9 ± 28.8 %); diadem defect, d 18 (55.6 ± 25.8 %); pyriform heads and nuclear vacuoles (excluding diadems), d 21 (18.3 ± 17.6 and 20.8 ± 10.5 %, respectively); knobbed acrosomes, d 27 (11.6 ± 13.6 % ). Sperm morphology was followed through d 39, by which time all bulls were producing ≤50% abnormal cells (35.2 ± 8.0 %). We concluded that viability of epididymal/rete sperm was adversely affected by elevated testicular temperatures, as noted by lowered viability of cryopreserved semen, and that there is a sequence in appearance of abnormal cell types in repsponse to thermal insult of the testis.<br>Master of Science
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Libros sobre el tema "Semen"

1

Tubau, Iván. Semen. Alzira, Valencia: Edicions Bromera, 2003.

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Vitoshnev, S. Semen Budennyĭ. Minsk: OOO "Kuzʹma", 1998.

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Hrabovet͡sʹkyĭ, V. V. Semen Vysochan. Ivano-Frankivsʹk: Kafedra istoriï Ukraïny Prykarpatsʹkoho universytetu im. V. Stefanyka, 1995.

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Galen. On semen. Berlin: Akademie Verlag, 1992.

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Demin, Lev Mikhaĭlovich. Semen Dezhnev. Moskva: "Molodai︠a︡ Gvardii︠a︡", 1990.

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Alʹtov, S. Semen Alʹtov. Moskva: Ėksmo, 2004.

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Ali͡ai͡ev, H. I͡E. Semen Frank. Sankt-Peterburg: "Nauka, ", 2017.

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Marrodán, Mario Angel. Semen de santo. Vigo, España: Ediciones Cardeñoso, 1989.

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Porus, V. N. Semen Li︠u︡dvigovich Frank. Moskva: ROSSPĖN, 2012.

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Torres, Cipriano. Semen y celindas. Murcia: Mestizo, 1994.

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Capítulos de libros sobre el tema "Semen"

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Cui, Yufeng, Bin Hu, Chenyue Dou, Yanyan Kang, Yongliang Ni, and Qifeng Nie. "Semen." In In Vitro Diagnostic Industry in China, 361–69. Singapore: Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-99-3110-1_21.

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Gooch, Jan W. "Semen." In Encyclopedic Dictionary of Polymers, 923. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_14771.

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Glover, T. D. "Semen Analysis." In Advances in Clinical Andrology, 15–29. Dordrecht: Springer Netherlands, 1988. http://dx.doi.org/10.1007/978-94-009-1237-3_3.

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Burch, Rebecca L. "Semen Familiarity." In Encyclopedia of Evolutionary Psychological Science, 1–2. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-16999-6_2012-1.

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Wolfner, Mariana F., and Tracey Chapman. "Semen Toxicity." In Encyclopedia of Evolutionary Psychological Science, 1–5. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-16999-6_3081-1.

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Taymor, Melvin L. "Semen Analysis." In Infertility, 89–98. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4613-0627-6_12.

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Cooper, Trevor G. "Semen Analysis." In Andrology, 125–38. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-540-78355-8_9.

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Ridley, John W. "Semen Evaluation." In Fundamentals of the Study of Urine and Body Fluids, 277–300. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-78417-5_12.

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Dawe-Long, Stuart Benjamin John. "Semen Analysis." In Atlas of Assisted Reproductive Technologies, 167–77. Singapore: Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-99-0020-6_9.

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Nordhoff, Verena, Elisabetta Baldi, Barbara Hellenkemper, and Eberhard Nieschlag. "Semen Analysis." In Andrology, 151–63. Cham: Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-31574-9_9.

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Actas de conferencias sobre el tema "Semen"

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Samková, Eva, Simona Janoušek Honesová, Karolína Reindl, and Jan Bárta. "PRODUKTY LNĚNÝCH SEMEN V MLÉČNÝCH VÝROBCÍCH / THE FLAXSEED PRODUCTS IN DAIRY PRODUCTS." In L. KONFERENCE O JAKOSTI POTRAVIN A POTRAVINOVÝCH SUROVIN / THE 50th FOOD QUALITY AND SAFETY CONFERENCE, 318–28. Mendel University in Brno, 2024. http://dx.doi.org/10.11118/978-80-7509-996-9-0318.

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Khalsum, Ummu, Fitriyanti Mayasari, and Ansar Suyuti. "Enhancement of Voltage Quality through DSTATCOM Implementation in the Electrical Infrastructure of Semen Tonasa." In 2024 4th International Conference of Science and Information Technology in Smart Administration (ICSINTESA), 353–57. IEEE, 2024. http://dx.doi.org/10.1109/icsintesa62455.2024.10747869.

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Grozea, Adrian, Melanca Lovrenschi, Violeta Igna, Ioana Grozea, and Silvia Patruica. "STUDY ON THE INFLUENCE OF WINTERING TEMPERATURE ON CERTAIN SEMEN CHARACTERISTICS IN STERLET (ACIPENSER RUTHENUS)." In SGEM International Multidisciplinary Scientific GeoConference, 121–30. STEF92 Technology, 2024. https://doi.org/10.5593/sgem2024v/6.2/s24.15.

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Sterlet (Acipenser ruthenus) is a species with seasonal reproduction, temperature being considered one of the most important key factors that ensures the seasonal reproductive cycle. The wintering temperature should be carefully controlled to prevent any adverse effects on reproduction. In many fish species, the wintering at high temperatures have a negative impact on reproduction success. The aim of this study is to evaluate the impact of wintering temperature on sterlet males by evaluating the quantity and quality of the semen collected from them during the breeding season. Two groups of males were overwintered at different temperatures, with minimums of 10 degrees C (G1) and 6 degrees C (G2), respectively. The semen collected from these males was analyzed, determining the volume of collected sperm, the concentration of spermatozoa and the number of spermatozoa, all of which were determined for two collections made at an interval of 2 hours. Gonadal measurements were also taken to determine if there were any differences between the two groups. The results showed that the volume of milt, sperm concentration, as well as gonad mass and the gonadosomatic index, did not differ significantly between the two groups. Thus, it can be concluded that wintering of sterlet males at temperatures no lower of 10 degrees C does not negatively influence the reproductive characteristics analyzed, showing only insignificant differences compared to males wintered at lower temperatures.
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Dehghan, Massoud, Ahmad Kalhor, Mohammad Hossein Nasr-Esfahani, and Marziyeh Tavalaee. "Predicting Sperm DNA Fragmentation Markers using Basic Semen Parameters and Age: A Preliminary Data Analysis." In 2025 29th International Computer Conference, Computer Society of Iran (CSICC), 1–7. IEEE, 2025. https://doi.org/10.1109/csicc65765.2025.10967411.

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张, 野., 河向 和, 向阳 余, 烨帆 蔡, 心影 彭, 伟槟 洪, and 玉杰 沈. "A study on the detection of authenticity of Cuscutae Semen based on hyperspectral imaging technology." In Tenth Symposium on Novel Optoelectronic Detection Technology and Applications, edited by Chen Ping, 126. SPIE, 2025. https://doi.org/10.1117/12.3056732.

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Jiang, Yufeng, Shiying Zheng, Chunyin Yan, and Wei Zhang. "The application of computer-assisted semen analysis parameters in semen quality analysis." In 2009 International Conference on Future BioMedical Information Engineering (FBIE). IEEE, 2009. http://dx.doi.org/10.1109/fbie.2009.5405901.

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Hong-yu, Li, Zhang Li-hua, Yuan Guang-xin, Zhou Yan-ju, Zhang Xue-li, Kong De-xin, Song Jia-yin, and Li Wei. "Research for ultramicrofingerprint of Semen Cuscutae." In 2011 International Conference on Human Health and Biomedical Engineering (HHBE). IEEE, 2011. http://dx.doi.org/10.1109/hhbe.2011.6027895.

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TKACHEV, Alexander V., Olga L. TKACHEVA, Lyudmila V. GAZZAVI-ROGOZINA, Tatyana V. ZUBOVA, Oksana V. SMOLOVSKAYA, and Vladimir A. PLESHKOV. "Modern Technology of Poultry Semen Cryopreservation." In IV International Scientific and Practical Conference "Modern S&T Equipments and Problems in Agriculture". Sibac, 2020. http://dx.doi.org/10.32743/kuz.mepa.2020.235-244.

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Zheng, Guang, Anjie Qiao, and Cheng Lv. "Therapeutic Mechanisms of Persicae Semen towards Healing Processes." In 2019 IEEE International Conference on Bioinformatics and Biomedicine (BIBM). IEEE, 2019. http://dx.doi.org/10.1109/bibm47256.2019.9044450.

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Chaudhari, Nandini M., and Bhausaheb V. Pawar. "Light scattering study on semen analysis methods/techniques." In 2013 Nirma University International Conference on Engineering (NUiCONE). IEEE, 2013. http://dx.doi.org/10.1109/nuicone.2013.6780079.

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Informes sobre el tema "Semen"

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Tummaruk, Padet, Kakanang Buranaamnuay, Wanpen Adulyanubap, Jinda Singlor, and Mongkol Techakumphu. Development of techniques for freezing boar semen. Chulalongkorn University, 2006. https://doi.org/10.58837/chula.res.2006.91.

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The objective of the present study was to investigate the feasibility of boar semen cryopreservation in Thailand and to investigate factors that could affect the boar semen quality after cryopreservation. Fifteen purebred boars (5 Landrace, 5 Yorkshire and 5 Duroc) from 2 commercial herds in Nakorn-prathom province were used in the experiment. Three ejaculates from each boar were collected with over one-week interval using gloved-hand technique. After collection, the semen was diluted with isothermal Beltsville thawing solution (BTS) extender. Diluted semen was held at 15 °C for 2 h and centrifuged. The supernatant was discarded and the semen precipitant was re-suspended with lactose-egg yolk (LEY) extender. The diluted semen was cool down to 5°C within 90 min. Two parts of semen were mixed with one part of LEY extender with 9% glycerol and 1.5% Equex-STM®. Thereafter, the processed semen was loaded into 0.5 mL straws. The straws were placed in liquid nitrogen (LN2) vapor approximately 3 cm above the level of the LN2 for 20 min and then were plunged into LN2. Thawing was achieved by immersing the straws in water at 50 °C for 12 second. After thawing, the semen was diluted (1:4) with an extender consisting of 95% BTS and 5% LEY extender. The extended thawed semen was incubated in a 38 °C water-bath for 30 min before evaluating the semen quality after thawing. Sperm concentration, individual motility, sperm viability, percentage of normal apical ridge (NAR), sperm plasma membrane function (sHost) and sperm plasma membrane integrity (SYBR) were evaluated. The semen qualities before and after thawing were compared for each boar using paired t-test. Pearson’s correlation was used to evaluate the correlation among all sperm parameters that were measured. Data on individual motility, viability, NAR, sHost positive spermatozoa and SYBR after thawing were analyzed using the General Linear Model (GLM) procedure of the SAS. On average, the sperm concentration of pre-diluted fresh semen was 529.7x106 spz/mL and the sperm concentration of frozen thawed semen was 811x106 spz/mL. The individual motility, the sperm viability, the NAR, the sHost and the SYBR of boar spermatozoa after frozen-thawed were 28%, 36.2%, 26.4%, 18.5% and 30.9%, respectively. All of the sperms parameters measured significantly decreased after frozen and thawed i.e., individual motility decreased by 44.2%, sperm viability decreased by 37.9% and NAR deceased by 59.5%. The individual motility of frozen-thawed spermatozoa was significantly correlated with the sperm viability (P&lt;0.001), the NAR (P&lt;0.02), the sHOST (P&lt;0.001) and the membrane integrity (SYBR) (P&lt;0.001). The higher concentration of frozen-thawed semen resulted in the lower membrane integrity (r=-0.3, P=0.04). Breed of boars and the individual boar within the same breed significantly influenced most of the sperm parameters after being frozen-thawed. The sperm viability of frozen-thawed semen in Duroc and Landrace boars was significantly higher than Yorkshire boars (P&lt;0.05). The membrane integrity of frozen-thawed semen in Landrace boar was significantly higher than Yorkshire boars (P&lt;0.05). In conclusions, the semen cryopreservation of boar could be performed successfully in Thailand with an average concentration of spermatozoa in frozen thawed semen of 811x106 spz/mL, the individual motility of 28% and the sperm viability of 36%. Breed of boars and the individual boar within the same breed significantly influenced most of the sperm parameters after being frozen-thawed.
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Dzhoglov, Spas, Doychin Boyadzhiev, and Evgeniya Neshova Ivanova. Association between Some Environment and Lifestyle Factors with Male Semen Quality Parameters: Semen Volume, Spermatozoa Concentra tion and Motility. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, January 2021. http://dx.doi.org/10.7546/crabs.2021.01.15.

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Weller, Joel, Harris Lewin, Micha Ron, and George Wiggans. Detection and Mapping of Genes Affecting Traits of Economic Importance in Dairy Cattle with the Aid of Molecular Genetic Markers. United States Department of Agriculture, December 1995. http://dx.doi.org/10.32747/1995.7613024.bard.

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Forty-seven poly-TG microsatellites were developed at the U of IL, and 11 genetic markers were developed at ARO, nine of which were poly-AGC microsatellites. Markers were typed on the reference families of CSIRO, Australia; GRANADA, Texas; and IRRF, Illinois, for chromosome assignment and linkage mapping. Nine North American al organizations contributed semen to the Dairy Bull DNA Repository (DBDR), which currently has 65,743 units from 3366 bulls. Semen was obtained for 31 out of 35 grandsires. Semen of 28 and 23 sons of two Israeli bulls was also collected. Eighteen grandsires were genotyped for 75 microsatellites. One thousand, three hundred and sixty-two sons with evaluation from 17 families were genotyped for 24 markers. Eleven thousand, six hundred and twenty sons genotypes were determined, of which 8,802 were informative. The genotype data was matched to the bulls' daughter yield deviations (DYD) for seven traits; milk, fat, and protein production; fat and protein percent; somatic cell concentration (SCS); and productive herd life. Seven loci had significant effects at p&lt;0.05, but only two loci, TGLA263 and MGTG7, had significant effects at p&lt;0.01, and the effect of TGLA263 on fat percentage was significant at p&lt;0.0001. There was at least one significant effect for each of the seven traits analyzed.
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Mabry, John. Comparison of Semen Delivery Systems for Commercial Swine Production in Iowa. Ames (Iowa): Iowa State University, January 2004. http://dx.doi.org/10.31274/ans_air-180814-97.

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Ouzounova-Raykova, Vessela, Radoslav Baykushev, Mohamed El Tibi, Simeon Rangelov, Dragomir Petrov, and Ivan Mitov. Genital Mycoplasmas and Ureaplasmas and Abnormal Semen Quality in Infertile Bulgarian Men. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, July 2018. http://dx.doi.org/10.7546/crabs.2018.07.15.

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Phillips, Patrick E., Jacqueline N. Myers, and Grant A. Dewell. Viability of Tritrichomonas foetus Following the Freeze-Thaw Cycle Used for Freezing Bovine Semen. Ames (Iowa): Iowa State University, January 2016. http://dx.doi.org/10.31274/ans_air-180814-571.

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Wang, Shunwei, Dingying Tu, Yinghua Zhang, Yourong Mao, and Jun Chen. Association of testicular microlithiasis with semen quality : a systematic review and a meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, March 2024. http://dx.doi.org/10.37766/inplasy2024.3.0075.

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Wen, Lequan, Haokun Tian, Xing Huang, Tiangang Song, Lirui Tang, Wenjie Wei, Shuo Tian, Yan Huang, and Xu Zhang. Effect of SARS-CoV-2 on semen parameters: A meta-analysis of 39 articles from 15 countries. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, September 2023. http://dx.doi.org/10.37766/inplasy2023.9.0002.

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Wen, Lequan, Haokun Tian, Xing Huang, Tiangang Song, Lirui Tang, Wenjie Wei, Shuo Tian, Yan Huang, and Xu Zhang. Effect of SARS-CoV-2 on semen parameters: A meta-analysis of 39 articles from 15 countries. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, February 2024. http://dx.doi.org/10.37766/inplasy2024.2.0083.

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Tummaruk, Padet, Peerapong Sumransarp, Wanpen Adulyanubap, Jinda Singlor, and Mongkol Techakumphu. Fertilization rate after deep intra uterine insemination in pig. Chulalongkorn University, 2006. https://doi.org/10.58837/chula.res.2006.92.

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The objectives of the present study were to investigate fertilization rate and number of embryo on each side of the reproductive tract after inseminated multiparous sows using deep intra uterine insemination (DIUI) technique with fresh semen. Eight crossbred multiparous sows were used in the experiment. The sows were examined for the onset of standing oestrus every 6 h by using a back pressure test in the presence of a mature boar. Transrectal ultrasonography was performed every 4 h using a 5 MHz probe to examine the time when ovulation took place in all sows. During the second oestrus after weaning, the sows were inseminated once at about 6-8 h before expected ovulation using diluted fresh semen from a proven sire. The insemination was performed using DIUI technique. The long flexible catheter (1.8 m) was inserted through the conventional artificial insemination (AI) catheter and was moved forward along one uterine horn until its total length. The diluted fresh semen with 150x106 motile sperm in 5.0 mL Beltsville thawing solution (BTS) was deposited at the proximal third in one side of the uterine horn (unknown side). Subsequently, a warm BTS 2.5 mL was used to flush the semen into the uterine horn after insemination. All sows (n=8) were generally anesthetized at about 61.1±12 h after insemination (range 48-72 h). The embryos were flushed from the oviduct and the proximal part of the uterine horn. The number of embryos and number of ovulation on the left and right sides of the reproductive tract within animal were compared using pair t-test. It was found that fertilization was found in 5 out of 8 sows (62.5%) after DIUI. On average, 11.2±2.2 embryos/sows were recovered from both sides of the reproductive tracts. The number of ovulation per sows was 16.4±2.6 ova. The number of ovulation on the left and the right side of the ovaries did not differ significantly (8.5 vs 7.9; P=0.75). Of the 5 pregnant sows, 57 embryos were recovered. Of these embryos, 3 embryos developed to 8-cell stages (5.3%) and 54 embryos developed to 4-cell stages (94.7%). Number of embryos recovered from the left and the right side of the reproductive tracts were not significantly differences (left-right =+4.2, P=0.20). The overall recovery rate of the oocytes and embryos was 66.4% (87/131). Fertilization rate in all pregnant sows was 100% (all ovum were fertilized). It could be concluded that using DIUI technique in pig with 150x106 spermatozoa, fertilization took place in both side of the oviducts.
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