Literatura académica sobre el tema "Viruses Polymerase chain reaction"

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Artículos de revistas sobre el tema "Viruses Polymerase chain reaction"

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Nazir, Iffat, Hafiz Zaid Mahmood, and Sana E Mustafa. "Polymerase chain reaction: a creative review." Journal of Applied Biotechnology & Bioengineering 7, no. 4 (2020): 157–59. http://dx.doi.org/10.15406/jabb.2020.07.00228.

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In molecular biology, a scientific technique PCR (polymerase chain reaction) is used to generate thousands to millions of copies of a single particular DNA sequences to amplify a single or few copies of a piece of DNA across several orders of magnitude. For multiple applications, PCR is an ordinary and often vital practice used in medicinal and biological research labs and is used for diagnosis and investigation of multiple diseases. In PCR mainly three major steps are involved. Denaturation, annealing, and extension. PCR can be used to detect not only the human genome but also the genome of v
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Taylor, K., and C. G. Copley. "Detection of rodent RNA viruses by polymerase chain reaction." Laboratory Animals 28, no. 1 (1994): 31–34. http://dx.doi.org/10.1258/002367794781065861.

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Piiparinen, H., and A. Vaheri. "Genotyping of herpes simplex viruses by polymerase chain reaction." Archives of Virology 119, no. 3-4 (1991): 275–83. http://dx.doi.org/10.1007/bf01310676.

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Shaw, Collie B., Neal Obermyer, Stephen J. Wetmore, George A. Spirou, and R. Wesley Farr. "Incidence of Adenovirus and Respiratory Syncytial Virus in Chronic Otitis Media with Effusion Using the Polymerase Chain Reaction." Otolaryngology–Head and Neck Surgery 113, no. 3 (1995): 234–41. http://dx.doi.org/10.1016/s0194-5998(95)70111-7.

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The aim of this study is to investigate the role of adenovirus and respiratory syncytial virus in the cause of chronic otitis media with effusion by use of the polymerase chain reaction for detection. The polymerase chain reaction has proved to be more sensitive and specific than viral cultures and immunoassays in the detection of viruses in other specimens. Adenovirus and respiratory syncytial virus were chosen because these viruses have been the most commonly isolated viruses in middle ear effusions in studies using other techniques. The effusions (132 total) were sterilely collected from 88
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Bowles, Neil E., Jiyuan Ni, Debra L. Kearney, et al. "Detection of viruses in myocardial tissues by polymerase chain reaction." Journal of the American College of Cardiology 42, no. 3 (2003): 466–72. http://dx.doi.org/10.1016/s0735-1097(03)00648-x.

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Li, Ruhui, Sarbagh Salih, and Suzanne Hurtt. "Detection of Geminiviruses in Sweetpotato by Polymerase Chain Reaction." Plant Disease 88, no. 12 (2004): 1347–51. http://dx.doi.org/10.1094/pdis.2004.88.12.1347.

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Geminivirus infection of sweetpotato (Ipomoea spp.) germplasm acquired from foreign regions is common. Graft inoculation of the indicator host, Ipomoea setosa, is the accepted detection method for these viruses, but the assay is laborious and requires up to 8 weeks. When infected sweetpotato is subjected to meristem tip culture to eliminate these viruses, the eradication rate is low. In this study, a polymerase chain reaction (PCR) detection assay was developed for the detection of geminiviruses in a variety of sweetpotato cultivars. Different methods were evaluated to extract nucleic acids su
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Gjøen, K. V., and A. L. Bruu. "Specific detection of Coxsackie viruses A by the polymerase chain reaction." Clinical and Diagnostic Virology 8, no. 3 (1997): 183–88. http://dx.doi.org/10.1016/s0928-0197(97)00017-2.

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Parra, B., and J. Slots. "Detection of human viruses in periodontal pockets using polymerase chain reaction." Oral Microbiology and Immunology 11, no. 5 (1996): 289–93. http://dx.doi.org/10.1111/j.1399-302x.1996.tb00183.x.

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Green, J., J. P. Norcott, D. Lewis, C. Arnold, and D. W. Brown. "Norwalk-like viruses: demonstration of genomic diversity by polymerase chain reaction." Journal of Clinical Microbiology 31, no. 11 (1993): 3007–12. http://dx.doi.org/10.1128/jcm.31.11.3007-3012.1993.

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Sheikh Ali, Hanan M., A. I. Khalafalla, and A. H. Nimir. "Detection of camel pox and vaccinia viruses by polymerase chain reaction." Tropical Animal Health and Production 41, no. 8 (2009): 1637–41. http://dx.doi.org/10.1007/s11250-009-9359-y.

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Tesis sobre el tema "Viruses Polymerase chain reaction"

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McMullen, Kevin Patrick. "Inhibitory effects of food matrices on real-time reverse transcription polymerase chain reaction detection of foodborne viruses." [Tampa, Fla. : s.n.], 2003. http://purl.fcla.edu/fcla/etd/SFE0000100.

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Wati, Satiya. "Rapid detection of Norwalk-like viruses (NLV's) /." Title page, contents and abstract only, 1999. http://web4.library.adelaide.edu.au/theses/09SM/09smw333.pdf.

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Feugeas, Olivier. "Pcr (polymerase chain reaction) et vih." Lille 2, 1990. http://www.theses.fr/1990LIL2M264.

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Mackay, Ian M. "Investigations into the utility of real-time PCR for the detection, quantitation and characterisation of clinically relevant viruses /." St. Lucia, Qld, 2003. http://adt.library.uq.edu.au/public/adt-QU20031120.155312/index.html.

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Lam, Yiu-pong, and 林耀邦. "Performance evaluation of the automated NucliSens easyMAG and Qiagen EZ1 Advanced XL nucleic acid extraction platform for detection of RNAand DNA viruses in clinical samples." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B46448020.

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Nunez, Desmond A. "The prevalence of human papilloma viruses in laryngeal squamous cell carcinoma : a polymerase chain reaction investigation." Thesis, University of Leicester, 1998. http://hdl.handle.net/2381/29552.

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Walia, Charanjiv Singh, University of Western Sydney, of Science Technology and Environment College, and of Science Food and Horticulture School. "Development of a method for the identification of novel viruses in marsupials with the polymerase chain reaction (PCR)." THESIS_CSTE_SFH_Walia_C.xml, 2002. http://handle.uws.edu.au:8081/1959.7/815.

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Four main types of viruses capable of causing systemic and gastrointestinal infections, namely Coronavirus, Rotavirus, Parvovirus or Morbillivirus (Tennant et al, 1991) have been investigated in marsupials. A pilot study to develop and optimise the methodology was undertaken using Canine Coronavirus and the study was then extended to marsupials and other target viruses.In the marsupial portion of the study, a fragment of the correct size for the amplification of pol gene, 409 bp, was obtained from two different faecal samples from tammar wallaby (from Macquarie Fauna Park) and one western grey
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Walia, Charanjiv Singh. "Development of a method for the identification of novel viruses in marsupials with the polymerase chain reaction (PCR) /." View thesis View thesis View thesis, 2002. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20030422.101329/index.html.

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劉永棠 and Wing-tong Ricky Lau. "Multiplex RT-PCR for typing and subtyping influenza and respiratory syncytial viruses." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2002. http://hub.hku.hk/bib/B31970667.

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Lau, Wing-tong Ricky. "Multiplex RT-PCR for typing and subtyping influenza and respiratory syncytial viruses." Hong Kong : University of Hong Kong, 2002. http://sunzi.lib.hku.hk/hkuto/record.jsp?B25151599.

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Libros sobre el tema "Viruses Polymerase chain reaction"

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Becker, Yechiel, and Gholamreza Darai, eds. Diagnosis of Human Viruses by Polymerase Chain Reaction Technology. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84766-0.

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Liu, Jiang Jian. Detection of bovine viral diarrhea virus by polymerase chain reaction. University of Prince Edward Island, 1990.

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Zhou, Chengfeng. Detection of infectious pancreatic necrosis virus by polymerase chain reaction. University of Prince Edward Island, 1992.

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Zhou, Chengfeng. Detection of infectious pancreatic necrosis virus by polymerase chain reaction. National Library of Canada, 1992.

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Liu, Jiang Jian. Detection of bovine viral diarrhea virus by polymerase chain reaction. National Library of Canada, 1990.

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Khodari, Yousif Abdulwahed Mohammad. Quantification of Herpes simplex virus type 1(HSV-1) DNA by the polymerase chain reaction. University of Manchester, 1995.

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Ahmed, Monzur. The application of the polymerase chain reaction (PCR) to the detection of Hepatitis C virus. University of Birmingham, 1997.

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Smith, Lee Martin. Development and application of a polymerase chain reaction test for the HPRS-103 strain ofavian leukosis virus. University of Birmingham, 1996.

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Murphy, Karen. Helpers simplex virus encephalitis: Establishment and evaluation of the polymerase chain reaction to overcome present diagnostic difficulties. The Author], 1995.

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Asia, World Health Organization Regional Office for South-East. Establishment of PCR laboratory in developing countries. World Health Organization, Regional Office for South-East Asia, 2011.

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Capítulos de libros sobre el tema "Viruses Polymerase chain reaction"

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Skern, Tim, Heinrich Kovar, Gunhild Jug, et al. "Polymerase Chain-Reaction (PCR) Detection of Rhinoviruses." In Diagnosis of Human Viruses by Polymerase Chain Reaction Technology. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84766-0_21.

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Stohwasser, Ralf, Lutz B. Giebel, Karl Raab, E. K. F. Bautz, and Gholamreza Darai. "Polymerase Chain Reaction for Detection of Hantaviruses." In Diagnosis of Human Viruses by Polymerase Chain Reaction Technology. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84766-0_28.

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Tordo, N., H. Bourhy, and D. Sacramento. "Polymerase Chain Reaction Technology for Rabies Virus." In Diagnosis of Human Viruses by Polymerase Chain Reaction Technology. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84766-0_29.

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Mahalingam, Ravi, Randall Cohrs, Aud N. Dueland, and Donald H. Gilden. "Polymerase Chain Reaction Diagnosis of Varicella Zoster Virus." In Diagnosis of Human Viruses by Polymerase Chain Reaction Technology. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84766-0_11.

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Mangano, Mark F., Richard L. Hodinka, and Jordan G. Spivack. "Detection of Human Cytomegalovirus by Polymerase Chain Reaction." In Diagnosis of Human Viruses by Polymerase Chain Reaction Technology. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84766-0_12.

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Ho, Linda, and George Terry. "Diagnosis of Prenatal Rubella by Polymerase Chain Reaction." In Diagnosis of Human Viruses by Polymerase Chain Reaction Technology. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84766-0_18.

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Clewley, Jonathan P. "Polymerase Chain Reaction Diagnosis of Human Parvovirus B19." In Diagnosis of Human Viruses by Polymerase Chain Reaction Technology. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84766-0_22.

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Allard, Annika, and Göran Wadell. "Polymerase Chain Reaction for the Detection of Adenoviruses." In Diagnosis of Human Viruses by Polymerase Chain Reaction Technology. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84766-0_23.

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Stewart, Janet N., Samir Mounir, and Pierre J. Talbot. "Detection of Coronaviruses by the Polymerase Chain Reaction." In Diagnosis of Human Viruses by Polymerase Chain Reaction Technology. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84766-0_24.

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Muranyi, Walter, and Rolf M. Flügel. "Detection of Human Spumaviruses by Polymerase Chain Reaction." In Diagnosis of Human Viruses by Polymerase Chain Reaction Technology. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84766-0_5.

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Actas de conferencias sobre el tema "Viruses Polymerase chain reaction"

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Lien, Kang-Yi, Wan-Chi Lee, Huan-Yao Lei, and Gwo-Bin Lee. "Micro Reverse Transcription Polymerase Chain Reaction Systems Using Super-paramagnetic Beads for Virus Detection." In 2006 1st IEEE International Conference on Nano/Micro Engineered and Molecular Systems. IEEE, 2006. http://dx.doi.org/10.1109/nems.2006.334869.

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Badeni, Sanaullah, Alam Mengal, Hajirah Dotani, et al. "Prevalence and diagnosis of Hepatitis B virus based on Polymerase Chain Reaction (PCR) in district Noshki Balochistan." In 2014 11th International Bhurban Conference on Applied Sciences and Technology (IBCAST). IEEE, 2014. http://dx.doi.org/10.1109/ibcast.2014.6778125.

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Stoicescu, Ramona, Razvan-Alexandru Stoicescu, Codrin Gheorghe, Adina Honcea, and Iulian Bratu. "CONSIDERATIONS ON SARS-COV-2 DIAGNOSIS IN THE LABORATORY OF UNIVERSITY EMERGENCY CLINICAL HOSPITAL OF CONSTANTA." In GEOLINKS Conference Proceedings. Saima Consult Ltd, 2021. http://dx.doi.org/10.32008/geolinks2021/b1/v3/07.

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Coronaviruses are members of the Coronaviridae family. They are enveloped, non-segmented, positive-sense, single-stranded RNA viruses. Their genome size is about 30 kilobases (kb) which consist, at the 5’ end, of non-structural open reading frames (ORFs: ORF1a, ORF 1b) which code for 16 non structural proteins, and at the 3’ end the genes which code for four structural proteins including membrane (M), envelope (E), spike (S), and nucleocapsid (N) proteins. Due to the rapid spread of COVID-19, a reliable detection method is needed for patient diagnosis especially in the early stages of the dise
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Pei, Shao Ning, Yi-Lun Wang, Chih-Ting Lin, and Ming C. Wu. "Isothermal real-time polymerase chain reaction detection of Herpes Simplex Virus Type 1 on a light-actuated digital microfludics platform." In 2013 Transducers & Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII). IEEE, 2013. http://dx.doi.org/10.1109/transducers.2013.6627385.

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Feng, Jun-li, and Ji-shuang Chen. "Determination Suppressive Effect of Satellite 369 on Accumulation of Cucumber Mosaic Virus by Real-Time Reverse Transcript-Polymerase Chain Reaction." In 2008 2nd International Conference on Bioinformatics and Biomedical Engineering (ICBBE '08). IEEE, 2008. http://dx.doi.org/10.1109/icbbe.2008.102.

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Zambrano, Héctor, Jesse Waggoner, Karina León, et al. "LB1.66 Detection of zika virus and cytomegalovirus in cervical cytology samples of pregnant women from guayaquil, ecuador, using two real-time polymerase chain reaction (RT-PCR) molecular assays." In STI and HIV World Congress Abstracts, July 9–12 2017, Rio de Janeiro, Brazil. BMJ Publishing Group Ltd, 2017. http://dx.doi.org/10.1136/sextrans-2017-053264.171.

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Persat, Alexandre, Tomoyuki Morita, and Juan G. Santiago. "On-Chip Isothermal Polymerase Chain Reaction." In ASME 2007 International Mechanical Engineering Congress and Exposition. ASMEDC, 2007. http://dx.doi.org/10.1115/imece2007-43070.

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We present a novel technique for on-chip PCR where temperature is held constant and uniform in the reactor. Specific chemicals, known as denaturants, have the ability to melt DNA. A flow control scheme establishes spatio-temporal fluctuations in the concentration of denaturants along a microchannel, while electromigration drives DNA through this spatially varying denaturant concentration field. Preliminary results show denaturation and extension of a 200 base pairs (bp) DNA template.
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Costa, Gabriel Sousa Silva, Anselmo C. Paiva, Geraldo Braz Júnior, and Marco Melo Ferreira. "COVID-19 automatic diagnosis with CT images using the novel Transformer architecture." In Simpósio Brasileiro de Computação Aplicada à Saúde. Sociedade Brasileira de Computação - SBC, 2021. http://dx.doi.org/10.5753/sbcas.2021.16073.

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Even though vaccines are already in use worldwide, the COVID-19 pandemic is far from over, with some countries re-establishing the lockdown state, the virus has taken over 2 million lives until today, being a serious health issue. Although real-time reverse transcription-polymerase chain reaction (RTPCR) is the first tool for COVID-19 diagnosis, its high false-negative rate and low sensitivity might delay accurate diagnosis. Therefore, fast COVID-19 diagnosis and quarantine, combined with effective vaccination plans, is crucial for the pandemic to be over as soon as possible. To that end, we pr
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Lin, Yu-Cheng, and Hua-Lin Wu. "Nanoparticle-Assisted High Efficient Polymerase Chain Reaction." In 2008 Second International Conference on Integration and Commercialization of Micro and Nanosystems. ASMEDC, 2008. http://dx.doi.org/10.1115/micronano2008-70214.

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This paper reports that the Au nanoparticles could dramatically enhance efficiency of polymer chain reaction (PCR) and shorten reaction time. The excellent energy transfer property of the nanoparticles should be the major factor in improving the PCR efficiency. With the addition of 0.7 nM of 13 nm Au nanoparticles into the PCR reagent, the PCR efficiency was increased. The results demonstrated that Au nanoparticles increase the sensitivity of PCR detection 5–10 fold in a slower PCR system (i.e. conventional PCR) and at least 104 fold in a quicker PCR system (i.e. real-time PCR). This innovatio
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Huang, Hai-Hui, G. E. Hedrick, Robert Burnap, and Haobo Liu. "Genome-wide polymerase chain reaction primer design." In the 2000 ACM symposium. ACM Press, 2000. http://dx.doi.org/10.1145/335603.335697.

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Informes sobre el tema "Viruses Polymerase chain reaction"

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Burke, Allen P. Detection and Clinicopathologic Correlation of Human Immunodeficiency Virus (HIV-1) Nucleic Acids and Antigens in Reticuloendothelial and Central Nervous System Tissues, by Immunohistochemistry, in situ Hybridization, and Polymerase Chain Reaction. Defense Technical Information Center, 1992. http://dx.doi.org/10.21236/ada259307.

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O'Leary, Timothy J., Melanie Cushion, Cynthia Wright, Thomas Fanning, and Mark Tsai. Polymerase Chain Reaction Based Diagnostic Assays for Pneumocystis Carinii. Defense Technical Information Center, 1992. http://dx.doi.org/10.21236/ada248259.

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Black, Jonathan. Quantitative Real-Time Polymerase Chain Reaction (qPCR) of Filamentous Fungi in Carpet. RTI Press, 2009. http://dx.doi.org/10.3768/rtipress.2009.mr.0011.0909.

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Churchill, M. E., M. A. Gemmell, and G. E. Woloschak. Polymerase chain reaction detection of retinoblastoma gene deletions in paraffin-embedded mouse lung adenocarcinomas. Office of Scientific and Technical Information (OSTI), 1991. http://dx.doi.org/10.2172/10173425.

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Arnett, Clint M., Giselle Rodriguez, and Stephen W. Maloney. Polymerase Chain Reaction (PCR) Analysis of Microbial Consortia on Wastewater Treatment Processes for High Explosives. Defense Technical Information Center, 2009. http://dx.doi.org/10.21236/ada544671.

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Zhang, N. Automation and integration of polymerase chain reaction with capillary electrophoresis for high throughput genotyping and disease diagnosis. Office of Scientific and Technical Information (OSTI), 1999. http://dx.doi.org/10.2172/348906.

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Denaro, Tracy R., Sarah K. Chelgren, Jara N. Lang, Ellen M. Strobel, Lori M. T. Balster, and Marlin D. Vangsness. DNA Isolation of Microbial Contaminants in Aviation Turbine Fuel via Traditional Polymerase Chain Reaction (PCR) and Direct PCR. Preliminary Results. Defense Technical Information Center, 2005. http://dx.doi.org/10.21236/ada446701.

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Zhelev, Doncho V., Christopher Dupuis, Suelynn Ren, Anna Le, Mia Hunt, and Henry Gibbons. Single Nucleotide Polymorphisms (SNP)-specific Quantitative Real Time Polymerase Chain Reaction (PCR) Assay for Analyzing Competition and Emergence of the Military Hypersporulating Strains of Bacillus Atrophaeous var. Globigii. Defense Technical Information Center, 2012. http://dx.doi.org/10.21236/ada570597.

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Lance, Richard, and Xin Guan. Variation in inhibitor effects on qPCR assays and implications for eDNA surveys. Engineer Research and Development Center (U.S.), 2021. http://dx.doi.org/10.21079/11681/41740.

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Aquatic environmental DNA (eDNA) surveys are sometimes impacted by polymerase chain reaction (PCR) inhibitors. We tested varying concentrations of different inhibitors (humic, phytic, and tannic acids; crude leaf extracts) for impacts on quantitative PCR (qPCR) assays designed for eDNA surveys of bighead and silver carp (Hypophthalmichthys nobilis and Hypophthalmichthys molitrix). We also tested for inhibition by high concentrations of exogenous DNA, hypothesizing that DNA from increasingly closely related species would be increasingly inhibitory. All tested inhibitors impacted qPCR, though on
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