Literatura académica sobre el tema "Wheat Disease and pest resistance"
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Artículos de revistas sobre el tema "Wheat Disease and pest resistance"
1
Bhatta, Madhav, Alexey Morgounov, Vikas Belamkar, Stephen N. Wegulo, Abdelfattah A. Dababat, Gül Erginbas-Orakci, Mustapha El Bouhssini, et al. "Genome-Wide Association Study for Multiple Biotic Stress Resistance in Synthetic Hexaploid Wheat." International Journal of Molecular Sciences 20, no. 15 (July 26, 2019): 3667. http://dx.doi.org/10.3390/ijms20153667.
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Genetic resistance against biotic stress is a major goal in many wheat breeding programs. However, modern wheat cultivars have a limited genetic variation for disease and pest resistance and there is always a possibility of the evolution of new diseases and pests to overcome previously identified resistance genes. A total of 125 synthetic hexaploid wheats (SHWs; 2n = 6x = 42, AABBDD, Triticum aestivum L.) were characterized for resistance to fungal pathogens that cause wheat rusts (leaf; Puccinia triticina, stem; P. graminis f.sp. tritici, and stripe; P. striiformis f.sp. tritici) and crown rot (Fusarium spp.); cereal cyst nematode (Heterodera spp.); and Hessian fly (Mayetiola destructor). A wide range of genetic variation was observed among SHWs for multiple (two to five) biotic stresses and 17 SHWs that were resistant to more than two stresses. The genomic regions and potential candidate genes conferring resistance to these biotic stresses were identified from a genome-wide association study (GWAS). This GWAS study identified 124 significant marker-trait associations (MTAs) for multiple biotic stresses and 33 of these were found within genes. Furthermore, 16 of the 33 MTAs present within genes had annotations suggesting their potential role in disease resistance. These results will be valuable for pyramiding novel genes/genomic regions conferring resistance to multiple biotic stresses from SHWs into elite bread wheat cultivars and providing further insights on a wide range of stress resistance in wheat.
2
Karsou, B., and R. Samara. "Plant Extracts Inducing Enzyme Activity in Grains Against Loose Smut Disease." Scientia Agriculturae Bohemica 52, no. 3 (September 1, 2021): 49–59. http://dx.doi.org/10.2478/sab-2021-0006.
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Abstract This study investigated the role of endogenous Palestinian plant extracts in inducing wheat and barley resistance systems against loose smut disease with the aim to alternate the chemical pest control with natural fungicides. Twenty indigenous herbal plant extracts and essential oils were assessed for their biological and antifungal properties against Ustilago tritici and Ustilago nuda. Their potential role in inducing resistance pathways was studied on four different cultivars of wheat and barley. Two common enzyme indicators – guaiacol peroxidase (POX) and polyphenol oxidase (PPO) – are expressed in plants only after physical or chemical induction. The antifungal activity of the plant extracts was investigated in vitro. Totally 70 % of the plant extracts showed antifungal activity against Ustilago tritici and Ustilago nuda. Coridothyme extracts ranked first (61 %) in the fungal growth inhibition, followed by varthemia, salvia, ambrosia, artemisia, and lemon thyme. Some plant extracts significantly increased the POX and PPO effect compared to control for all the wheat and barley cultivars tested. The study revealed that oregano, clove or lavender and pomegranate, common yarrow or chamomile oil effectively induced the resistance indicator enzymes in wheat and barley.
3
Fedak, G. "Molecular aids for integration of alien chromatin through wide crosses." Genome 42, no. 4 (August 1, 1999): 584–91. http://dx.doi.org/10.1139/g99-046.
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Wide crosses in wheat have now been performed for over 100 years. In that time, approximately 100 genes have been transferred for numerous traits, including biotic and abiotic stresses and value-added traits. Resistance genes from alien sources do become defeated with time, so the search for additional variability must continue. Recent screening of alien species has identified accessions with multiple pest resistance plus combinations of pest resistance and value-added traits. The majority of existing induced recombinants are of a noncompensating type with considerable linkage drag, so sequential useage of Ph mutants is recommended to produce smaller interstitial recombinants. Molecular methods, including GISH, RAPD, RFLP, AFLP, and microsatellites, are being widely used to identify integrated alien chromosomes, chromosome segments, and genes.Key words: Triticum aestivium, molecular markers, disease resistance, gene introgression, interspecific hybrids.
4
Krut’, M. V. "An overview of innovative developments from the scientific provision of plant selection to resistance to diseases and pests." Scientific Journal Grain Crops 5, no. 1 (2021): 23–29. http://dx.doi.org/10.31867/2523-4544/0154.
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The Institute of Plant Protection of NAAS developed methods of plant selection for resistance to major pathogens and assessment methodology the resistance of winter wheat, potatoes, clover and alfalfa to pests to create complex resistant varieties. Donors of potato resistance to cancer, Alternaria, Phomosis and cyst-forming nematodes were identified. Methods for determining the resistance of cereals to high and low temperatures were developed. A collection of the Aegilops biuncialis L. wild wheat samples as sources of new resistance genes to plant diseases and pests was compiled. The soft winter wheat resistance genes to diseases by DNA markers were identified at the Institute of Plant Production named after V. Ya. Yuriev. The V. M. Remeslo Myronivka Institute of Wheat formed a set of winter wheat cultivars with group and complex resistance to diseases and pests. The Institute of Oilseed Crops of NAAS established physiological and biochemical mechanisms of resistance of sunflower, soybean, crown flax to pathogens. Collections of sunflower lines based on complex resistance to sunflower broomrape, dry rot, downy mildew and soybean lines based on complex resistance to white rot and Anthracnose were also created. In the National Scientific Center "Institute of Agriculture of NAAS", the fodder lupine resistance to the most important pathogens was investi-gated. The Institute of Agriculture in the Carpathian Region of NAAS revealed the spring barley, oat, rape, fiber flax varieties and selection numbers resistant to basic diseases; and the Institute of Rice of NAAS – rice cultivars resistant to diseases and pest pathogens. The resistance to main phytophagous insects of the modern genotypes of hemp, fiber flax and crown flax was assessed by the Institute of Agriculture of the North-East of NAAS. The assessment method of breeding value for the initial material of the main vegetables on the basis of disease resistance was developed by the Institute of Vegetables and Melons Growing of NAAS. Scientists of the Institute of Agroecology and Environmental Management of NAAS and V. M. RemesloMyronivka Institute of Wheat of NAAS revealed the cucumber and barley resistance to viral diseases. Key words: crops, pests, pathogens, resistance, resistance gene, resistance source.
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Altaf, Adil, Amir Zaman Shah, Sadia Gull, Shahid Hussain, Muhammad Faheem, Ad Al Amin Miah, and Xinkai Zhu. "Progress in modern crop science research in wheat biology." Journal of Global Innovations in Agricultural Sciences 10, no. 1 (March 28, 2022): 43–49. http://dx.doi.org/10.22194/jgias/10.953.
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Every crop breeding effort seeks to enhance production. Despite several advances, most worldwide breeding efforts have long sought to improve grain production potential, insect/pest/disease resistance, grain quality, and stress tolerance. Almost all wheat breeding programs aim to increase grain yield potential. Wheat breeders have achieved substantial improvements in crop yield. Genetic transformation, cloning, and genetic engineering increase production potential in wheat. The primary breeding strategy for wheat is the pedigree. However, hybrids and population improvement are also utilized. Breeders utilized biotechnology to increase breeding success. Biotechnology and genome editing are examples of current technology that can improve global agriculture production by assisting crop development. Traditional wheat breeding methods have been supplemented with biotechnology to speed up wheat improvement efforts. These methods will speed up wheat biology research and help to develop wheat breeding plans. However, many programs in developing countries (especially) are still trying to include them.
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Jauhar, Prem P., and Ravindra N. Chibbar. "Chromosome-mediated and direct gene transfers in wheat." Genome 42, no. 4 (August 1, 1999): 570–83. http://dx.doi.org/10.1139/g99-045.
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Wild grasses, including relatives of wheat, have several desirable characters that can be introduced into both bread wheat and durum wheat. Since current wheat cultivars lack certain traits, for example, resistance to fusarium head blight (scab), related wild grasses may be the only option for useful variability. Wide hybridization of wheat with grasses, coupled with cytogenetic manipulation of the hybrid material, has been instrumental in the genetic improvement of wheat. Chromosome engineering methodologies, based on the manipulation of pairing control mechanisms and induced translocations, have been employed to transfer into wheat specific disease and pest resistance genes from annual (e.g., rye) or perennial (e.g., Thinopyrum spp., Lophopyrum spp., and Agropyron spp.) members of the wheat tribe, Triticeae. The advent of in situ hybridization techniques, for example, fluorescent GISH combined with Giemsa C-banding, has proved immensely useful in characterizing alien chromatin specifying resistance to various pathogens and pests. The use of DNA markers (RAPDs and RFLPs) helps to identify desirable genotypes more precisely and, thereby, facilitates gene transfer into wheat. Such markers may be particularly helpful in monitoring the introgression of alien genes in the wheat genome. In fact, several cultivars, particularly of bread wheat, contain superior traits of alien origin. The development of novel gene-transfer techniques in the past decade that allow direct delivery of DNA into regenerable embryogenic callus of wheat has opened up new avenues of alien-gene transfer into wheat cultivars. Thus, transgenic bread and durum wheats have been produced and methods of gene delivery standardized. The application of transgenic technology has not only yielded herbicide-resistant wheats, but has also helped to improve grain quality by modifying the protein and starch profiles of the grain. These in vitro approaches to gene transfer are developing rapidly, and promise to become an integral part of plant breeding efforts. However, the new biotechnological tools will complement, not replace, conventional plant breeding.Key words: alien-gene transfer, fluorescent GISH, Giemsa banding, homoeologous chromosome pairing, molecular markers, transgenic bread wheat, transgenic durum wheat.
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Gaurav, Kumar, Sanu Arora, Paula Silva, Javier Sánchez-Martín, Richard Horsnell, Liangliang Gao, Gurcharn S. Brar, et al. "Population genomic analysis of Aegilops tauschii identifies targets for bread wheat improvement." Nature Biotechnology 40, no. 3 (November 1, 2021): 422–31. http://dx.doi.org/10.1038/s41587-021-01058-4.
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AbstractAegilops tauschii, the diploid wild progenitor of the D subgenome of bread wheat, is a reservoir of genetic diversity for improving bread wheat performance and environmental resilience. Here we sequenced 242 Ae. tauschii accessions and compared them to the wheat D subgenome to characterize genomic diversity. We found that a rare lineage of Ae. tauschii geographically restricted to present-day Georgia contributed to the wheat D subgenome in the independent hybridizations that gave rise to modern bread wheat. Through k-mer-based association mapping, we identified discrete genomic regions with candidate genes for disease and pest resistance and demonstrated their functional transfer into wheat by transgenesis and wide crossing, including the generation of a library of hexaploids incorporating diverse Ae. tauschii genomes. Exploiting the genomic diversity of the Ae. tauschii ancestral diploid genome permits rapid trait discovery and functional genetic validation in a hexaploid background amenable to breeding.
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Kozub, N. A., I. A. Sozinov, A. Ya Bidnyk, N. A. Demianova, Ya B. Blume, and A. A. Sozinov. "Development of common wheat lines with the recombinant arm 1RS as a source of new combinations of disease and pest resistance genes." Interdepartmental Thematic Scientific Collection of Plant Protection and Quarantine, no. 62 (September 3, 2016): 143–50. http://dx.doi.org/10.36495/1606-9773.2016.62.143-150.
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A combination of recombinant-inbred lines of the F6 generation from the cross B-16 ќ AR 7086 between lines with two wheat-rye translocations, 1BL/1RS from the Petkus and 1AL/1RS from the rye Insave, was developed. Using gliadin and secalin loci as genetic markers we identified recombinant arm 1RS in positions 1A and 1B in about 10% of lines. The rest of lines with the rye material may also carry recombinant 1RS, which can be identified with DNA markers. Lines with recombinant arm 1RS may serve as a source of new combination of rye genes for disease and pest resistance.
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Junker, Yvonne, Sebastian Zeissig, Seong-Jun Kim, Donatella Barisani, Herbert Wieser, Daniel A. Leffler, Victor Zevallos, et al. "Wheat amylase trypsin inhibitors drive intestinal inflammation via activation of toll-like receptor 4." Journal of Experimental Medicine 209, no. 13 (December 3, 2012): 2395–408. http://dx.doi.org/10.1084/jem.20102660.
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Ingestion of wheat, barley, or rye triggers small intestinal inflammation in patients with celiac disease. Specifically, the storage proteins of these cereals (gluten) elicit an adaptive Th1-mediated immune response in individuals carrying HLA-DQ2 or HLA-DQ8 as major genetic predisposition. This well-defined role of adaptive immunity contrasts with an ill-defined component of innate immunity in celiac disease. We identify the α-amylase/trypsin inhibitors (ATIs) CM3 and 0.19, pest resistance molecules in wheat, as strong activators of innate immune responses in monocytes, macrophages, and dendritic cells. ATIs engage the TLR4–MD2–CD14 complex and lead to up-regulation of maturation markers and elicit release of proinflammatory cytokines in cells from celiac and nonceliac patients and in celiac patients’ biopsies. Mice deficient in TLR4 or TLR4 signaling are protected from intestinal and systemic immune responses upon oral challenge with ATIs. These findings define cereal ATIs as novel contributors to celiac disease. Moreover, ATIs may fuel inflammation and immune reactions in other intestinal and nonintestinal immune disorders.
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Morgounov, Alexey, Aygul Abugalieva, Kadir Akan, Beyhan Akın, Stephen Baenziger, Madhav Bhatta, Abdelfattah A. Dababat, et al. "High-yielding winter synthetic hexaploid wheats resistant to multiple diseases and pests." Plant Genetic Resources: Characterization and Utilization 16, no. 3 (May 12, 2017): 273–78. http://dx.doi.org/10.1017/s147926211700017x.
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AbstractDevelopment of winter wheat (Triticum aestivum) synthetics started at CIMMYT-Mexico in 2004, when winter durum wheat (Triticum turgidum) germplasm from Ukraine and Romania was crossed with Aegilops tauschii accessions from the Caspian Sea region. Chromosomes were doubled after pollination and embryo rescue, but chromosome number and cytological validation was not performed. F2 populations were grown in Mexico and were shipped to Turkey in 2008. During 2009–2015, these populations were subjected to rigorous pedigree selection under dry, cold, disease-affected environments of the Central Anatolian Plateau. The wide segregation and partial sterility observed in 2009 gradually decreased and, by 2016, most of the F8 single spike progenies demonstrated good fertility and agronomic performance. Since 2013, lines have been selected from synthetic populations and evaluated at multiple sites. Superior lines were characterized for resistance to leaf, stripe and stem rust, plant height, and reaction to common bunt and soil-borne pathogens. Thousand kernel weight of many lines exceeded 50 g, compared with the check varieties that barely reached 40 g. Threshability of synthetic lines varied from 0 to 95%, demonstrating genetic variation for this important domestication trait. Screening against Hessian fly, sunny pest and Russian wheat aphid identified several resistant genotypes. Both durum and Aegilops parents affected synthetic wheat traits. Several studies are underway to reveal the genetic diversity of synthetic lines and the basis of resistance to diseases and insects. This synthetic germplasm represents a new winter bread wheat parental pool. It is available upon request to interested breeding/research programmes.
Tesis sobre el tema "Wheat Disease and pest resistance"
1
Golegaonkar, Prashant G. "Genetic and molecular analysis of resistance to rust diseases in barley." Thesis, The University of Sydney, 2007. http://hdl.handle.net/2123/3549.
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The responses of 92 barley genotypes to selected P. hordei pathotypes was assessed in greenhouse tests at seedling growth stages and in the field at adult plant growth stages to determine known or unknown resistances. On the basis of multipathotype tests, 35 genotypes were postulated to carry Rph2, Rph4, Rph5, Rph12, RphCantala alone or combinations of Rph2 + Rph4 and Rph1 + Rph2, whereas 52 genotypes lacked detectable seedling resistance to P. hordei. Five genotypes carried seedling resistance that was effective to all pathotypes tested, of which four were believed to carry uncharacterised resistance based on pedigree information. Field tests at adult plant growth stages indicated that while 28 genotypes were susceptible, 57 carried uncharacterised APR to P. hordei. Pedigree analysis indicated that APR in the test genotypes could have been derived from three different sources. The resistant responses of seven cultivars at adult plant growth stages were believed to be due to the presence of seedling resistance effective against the field pathotypes. Genetic studies conducted on 10 barley genotypes suggested that ‘Vada’, ‘Nagrad’, ‘Gilbert’, ‘Ulandra (NT)’ and ‘WI3407’ each carry one gene providing adult plant resistance to P. hordei. Genotypes ‘Patty’, ‘Pompadour’ ‘Athos’, ‘Dash’ and ‘RAH1995’ showed digenic inheritance of APR at one field site and monogenic inheritance at a second. One of the genes identified in each of these cultivars provided high levels of APR and was effective at both field sites. The second APR gene was effective only at one field site, and it conferred low levels of APR. Tests of allelism between resistant genotypes confirmed a common APR gene in all genotypes with the exception of ‘WI3407’, which based on pedigree information was genetically distinct from the gene common in ‘Vada’, ‘Nagrad’, ‘Patty’, ‘RAH1995’ and ‘Pompadour’. An incompletely dominant gene, Rph14, identified previously in an accession of Hordeum vulgare confers resistance to all known pathotypes of P. hordei in Australia. The inheritance of Rph14 was confirmed using 146 and 106 F3 lines derived from the crosses ‘Baudin’/ ‘PI 584760’ (Rph14) and ‘Ricardo’/‘PI 584760’ (Rph14), respectively. Bulk segregant analysis on DNA from the parental genotypes and resistant and susceptible DNA bulks from F3 lines using diversity array technology (DArT) markers located Rph14 to the short arm of chromosome 2H. Polymerase chain reaction (PCR) based marker analysis identified a single simple sequence repeat (SSR) marker, Bmag692, linked closely to Rph14 at a map distance of 2.1 and 3.8 cM in the populations ‘Baudin’/ ‘PI 584760’and ‘Ricardo’/‘PI 584760’, respectively. Seedlings of 62 Australian and two exotic barley cultivars were assessed for resistance to a variant of Puccinia striiformis, referred to as BGYR, which causes stripe rust on several wild Hordeum species and some genotypes of cultivated barley. With the exception of six Australian barley cultivars and an exotic cultivar, all displayed resistance to the pathogen. Genetic analyses of six Australian barley cultivars and the Algerian barley ‘Sahara 3771’, suggested that they carried either one or two major seedling resistance genes to the pathogen. A single recessive seedling resistance gene, Bgyr1, identified in ‘Sahara 3771’ was located on the long arm of chromosome 7H and flanked by restriction fragment length polymorphism (RFLP) markers wg420 and cdo347 at genetic distances of 12.8 and 21.9 cM, respectively. Mapping resistance to BGYR at adult plant growth stages using a doubled haploid population derived from the cross ‘Clipper’/‘Sahara 3771’ identified two major QTLs on the long arms of chromosomes 3H and 7H that explained 26 and 18% of total phenotypic variation, respectively. The QTL located on chromosome 7HL corresponded to the seedling resistance gene Bgyr1. The second QTL was concluded to correspond to a single adult plant resistance gene designated Bgyr2, originating from cultivar ‘Clipper’.
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Golegaonkar, Prashant G. "Genetic and molecular analysis of resistance to rust diseases in barley." University of Sydney, 2007. http://hdl.handle.net/2123/3549.
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Doctor of Philosophy<br>The responses of 92 barley genotypes to selected P. hordei pathotypes was assessed in greenhouse tests at seedling growth stages and in the field at adult plant growth stages to determine known or unknown resistances. On the basis of multipathotype tests, 35 genotypes were postulated to carry Rph2, Rph4, Rph5, Rph12, RphCantala alone or combinations of Rph2 + Rph4 and Rph1 + Rph2, whereas 52 genotypes lacked detectable seedling resistance to P. hordei. Five genotypes carried seedling resistance that was effective to all pathotypes tested, of which four were believed to carry uncharacterised resistance based on pedigree information. Field tests at adult plant growth stages indicated that while 28 genotypes were susceptible, 57 carried uncharacterised APR to P. hordei. Pedigree analysis indicated that APR in the test genotypes could have been derived from three different sources. The resistant responses of seven cultivars at adult plant growth stages were believed to be due to the presence of seedling resistance effective against the field pathotypes. Genetic studies conducted on 10 barley genotypes suggested that ‘Vada’, ‘Nagrad’, ‘Gilbert’, ‘Ulandra (NT)’ and ‘WI3407’ each carry one gene providing adult plant resistance to P. hordei. Genotypes ‘Patty’, ‘Pompadour’ ‘Athos’, ‘Dash’ and ‘RAH1995’ showed digenic inheritance of APR at one field site and monogenic inheritance at a second. One of the genes identified in each of these cultivars provided high levels of APR and was effective at both field sites. The second APR gene was effective only at one field site, and it conferred low levels of APR. Tests of allelism between resistant genotypes confirmed a common APR gene in all genotypes with the exception of ‘WI3407’, which based on pedigree information was genetically distinct from the gene common in ‘Vada’, ‘Nagrad’, ‘Patty’, ‘RAH1995’ and ‘Pompadour’. An incompletely dominant gene, Rph14, identified previously in an accession of Hordeum vulgare confers resistance to all known pathotypes of P. hordei in Australia. The inheritance of Rph14 was confirmed using 146 and 106 F3 lines derived from the crosses ‘Baudin’/ ‘PI 584760’ (Rph14) and ‘Ricardo’/‘PI 584760’ (Rph14), respectively. Bulk segregant analysis on DNA from the parental genotypes and resistant and susceptible DNA bulks from F3 lines using diversity array technology (DArT) markers located Rph14 to the short arm of chromosome 2H. Polymerase chain reaction (PCR) based marker analysis identified a single simple sequence repeat (SSR) marker, Bmag692, linked closely to Rph14 at a map distance of 2.1 and 3.8 cM in the populations ‘Baudin’/ ‘PI 584760’and ‘Ricardo’/‘PI 584760’, respectively. Seedlings of 62 Australian and two exotic barley cultivars were assessed for resistance to a variant of Puccinia striiformis, referred to as BGYR, which causes stripe rust on several wild Hordeum species and some genotypes of cultivated barley. With the exception of six Australian barley cultivars and an exotic cultivar, all displayed resistance to the pathogen. Genetic analyses of six Australian barley cultivars and the Algerian barley ‘Sahara 3771’, suggested that they carried either one or two major seedling resistance genes to the pathogen. A single recessive seedling resistance gene, Bgyr1, identified in ‘Sahara 3771’ was located on the long arm of chromosome 7H and flanked by restriction fragment length polymorphism (RFLP) markers wg420 and cdo347 at genetic distances of 12.8 and 21.9 cM, respectively. Mapping resistance to BGYR at adult plant growth stages using a doubled haploid population derived from the cross ‘Clipper’/‘Sahara 3771’ identified two major QTLs on the long arms of chromosomes 3H and 7H that explained 26 and 18% of total phenotypic variation, respectively. The QTL located on chromosome 7HL corresponded to the seedling resistance gene Bgyr1. The second QTL was concluded to correspond to a single adult plant resistance gene designated Bgyr2, originating from cultivar ‘Clipper’.
3
Wilkes, Meredith Ann. "The Role Of Hydroxamic Acids In Take-all Resistance." Thesis, The University of Sydney, 1997. https://hdl.handle.net/2123/27618.
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The occurrence of hydroxamic acids (Hx) and their affects on take-all have
been investigated in this study. An improved HPLC procedure for the
separation and quantification of Hx in wheat, rye and triticale roots was
established. This method completely separated 2,4-Dihydroxy—1,4—
benzoxazin—3-one (DIBOA), 2 , 4-Dihydroxy - 7- methoxy - 1 4- benzoxazin
-3-one (DIMBOA), 2(3)-benzoxazolinone (BOA) and 6-
methoxybenzoxazolinone (MBOA) within 17 min.
DIMBOA was the only Hx found in wheat roots, whereas both DIMBOA
and DIBOA were present in the roots of triticale and rye. The Hx content of
whole roots of wheat, rye and triticale reached a maximum 3 to 4 days after
germination, depending on species. The DIMBOA content of wheat roots
ranged from 0.4 to 1.5 umoles / g f.wt in the varieties studied. The DIMBOA
content of the triticale varieties ranged from 0.9 to 2.0 umoles/ g f.wt, and
DIBOA from 0.26 to 1.1 umoles / g f.wt. DIMBOA concentrations in rye
roots ranged from 0.3 to 0.5 umoles/ g f.wt, whereas DIBOA levels ranged
from zero to 1.1 umoles/g.f.wt.
The Hx content of Wheat, rye and triticale roots was highest in the
youngest parts of the root. The root tip of these cereals always contained
significantly higher levels of Hx than the older parts of the root.
When extracts prepared from triticale and rye roots were incorporated into
the nutrient media, growth of two isolates of Gaeumannomyces graminis
var.tritici (th) (E31 and WP 28) was inhibited. Similar extracts prepared
from wheat did not inhibit the growth of th. The fungal strain WP 28
actually grew more rapidly on medium containing extracts from wheat
(cv. Sunstar) roots. The inhibitory effect of triticale and rye extracts was
attributed to the presence of DIBOA. The inhibitory effect of these particular extracts correlated to the resistance of the respective plant to take-all in the field as reported by Hollins et al . (1986).
Hydroxamic acids inhibited the growth of th when incorporated into the
growth media. DIBOA and BOA significantly inhibited the growth of both
strains of the fungus at concentrations as low as 0.5 mM. DIMBOA and 6-
methoxybenzoxazolinone (MBOA) did not significantly inhibit the growth
of th EBI at 0.5 mM. However, at higher concentrations DIMBOA and
MBOA were inhibitory. The Hx at the concentrations studied (0.5 to 5.0
mM) were only fungistatic, though, as the fungal colonies resumed
growth when removed from the inhibitor. There was no significant
difference in the growth of the two fungal isolates on media containing
extracts or Hx.
The wheat variety with the lowest DIMBOA content was the most
susceptible to infection by the fungus. Wheat contained only DIMBOA,
which was undetectable by 21 days. The cereals, rye and triticale, which
contined both DIMBOA and DIBOA were more resistant to the take-all
fungus. Hydroxamic acid levels in triticale and rye were low or not
detectable at 21 and 35 days. Rye was the more resistant species out of the
two. Increased synthesis of Hx was not observed in roots of these cereals as
a response to infection by the take—all fungus. On the basis of these results,
it was concluded that DIBOA was more effective than DIMBOA in
conferring resistance to take-all.
Wheat varieties which had an individual rye chromosome inserted were
assayed for Hx content. All lines contained DIMBOA but one line (CSB 5R)
also contained DIBOA. This preliminary result indicates that the gene(s)
responsible for DIBOA synthesis may be on chromosome 5 of rye.
4
Wellings, Colin Ross. "Host: pathogen studies of wheat stripe rust in Australia." Thesis, Department of Agricultural Genetics and Biometry, 1986. http://hdl.handle.net/2123/14544.
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Horn, Marizanne. "Transfer of genetic resistance to the Russian wheat aphid from rye to wheat." Thesis, Stellenbosch : Stellenbosch University, 1997. http://hdl.handle.net/10019.1/55770.
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Thesis (MSc.) -- Stellenbosch University, 1997.<br>ENGLISH ABSTRACT: An octoploid triticale was derived from the F1 of a Russian wheat aphid
resistant rye, 'Turkey 77', and 'Chinese Spring' wheat. The alloploid was
crossed (a) to common wheat, and (b) to the 'Imperial' rye to 'Chinese Spring'
disomic addition lines. F2 progeny from these crosses were tested for
Russian wheat aphid resistance and C-banded. Resistance was found to be
associated with chromosome arm 1RS of the 'Turkey 77' rye genome. This
initial work was done by MARAIS (1991) who made a RWA resistant,
monotelosomic 1RS ('Turkey 77') addition plant available for the study. The
F3 progeny of this monotelosomic addition plant was used to confirm the
RWA resistance on chromosome 1RS. The monotelosomic addition plant
was then crossed with the wheat cultivar 'Gamtoos', which has the 1BL.1 RS
'Veery' translocation. Unlike the 1RS segment in 'Gamtoos', the 'Turkey 77'-
derived 1RS telosome did not express the rust resistance genes 5r31 and
Lr26 which could then be used as markers. From the F1 a monotelosomic
1RS addition plant that was also heterozygous for the 1BL.1 RS translocation,
was selected and testcrossed with an aphid susceptible common wheat, 'Inia
66'. Meiotic pairing between the .rye arms resulted in the recovery of five
euploid, Russian wheat aphid resistant plants out of a progeny of 99
euploids. One recombinant also retained 5r31 and Lr26 and was allowed to
self pollinate. With the aid of SOS-PAGE profiles, Russian wheat aphid
resistant 1BL.1 RS translocation homozygotes were identified and it was
possible to confirm that the Russian wheat aphid resistance gene was in fact
transferred to the 1BL.1RS ('Veery') translocation.
Two attempts were made to map the Russiar, wheat aphid locus or loci.
(1) Telosomic mapping was attempted. For this purpose a plant with 2n =
40 + 1BL.1 RS + 1RS was obtained, and testcrossed with a Russian wheat
aphid susceptible wheat. (2) A disomic, recombined 1BL.1 RS translocation
line with Russian wheat aphid resistance but lacking the Lr26 and Sr31 alleles was crossed with 'Gamtoos' and the F1 testcrossed. The testcross in
both strategies were done with 'Chinese Spring'. In the first experiment the
Sr31 locus was located 10.42 map units from the Lr26 locus. The rust
resistance data implied that the genetic distance estimates may be unreliable
and therefore the laborious Russian wheat aphid resistance tests were not
done. In the second experiment a Russian wheat aphid resistance gene was
located 14.5 map units from the Lr26 locus. In the latter cross nonmendel
ian segregation of the Russian wheat aphid resistance evidently
occurred which implied that the estimated map distance may be inaccurate.
It was also not possible to determine the number of genes involved from the
data.<br>Digitized at 300 dpi Colour & b/W PDF format (OCR), using ,KODAK i 1220 PLUS scanner. Digitised, Ricardo Davids on request from ILL 25 April 2013<br>AFRIKAANSE OPSOMMING: 'n Oktaplo"lede triticale is gemaak vanaf die F1 van 'n kruising tussen 'n
Russiese koringluis-weerstandbiedende rog, 'Turkey 77', en die
koringkultivar 'Chinese Spring'. Die alloplo"led is gekruis met gewone
broodkoring en met 'Imperial' rog/'Chinese Spring' disomiese addissielyne.
Die F2 nageslag vanaf hierdie kruisings is getoets vir Russiese koringluisweerstandbiedendheid
en C-bande is ook gedoen. Weerstand is gevind wat
geassosieer is met die 1RS chromosoomarm van 'Turkey 77'. Hierdie
oorspronklike werk is deur MARAIS (1991) gedoen en uit sy materiaal is 'n
monotelosomiese 1RS ('Turkey 77') addissieplant beskikbaar gestel vir die
huidige studie. Die F3 nageslag van hierdie monotelosomiese addissieplant
is gebruik om die weerstand teen die Russiese koringluis op chromosoom
1RS te bevestig. Die monotelosomiese addissieplant is ook gekruis met die
koringkultivar 'Gamtoos' wat die 1BL.1 RS-translokasie dra. Hoewel die 1RS
segment van 'Gamtoos' die roesweerstandsgene, Sr31 en Lr26 uitdruk, is dit
nie die geval met die 'Turkey 77' 1RS telosoom nie. Hierdie gene kon dus as
merkergene gebruik word. Vanuit die F1 is 'n monotelosomiese 1RS
addissieplant geselekteer wat ook heterosigoties was vir die 1BL.1 RStranslokasie.
Hierdie plant is getoetskruis met 'n luisvatbare gewone
broodkoring, 'Inia 66'. Meiotiese paring tussen die rogarms het daartoe gelei
dat vyf euplo"lede Russiese koringluis-weerstandbiedende nageslag uit 99
euplo"lede nageslag geselekteer kon word. Een rekombinant het ook Sr31
en Lr26 behou en is toegelaat om self te bestuif. Met behulp van SDSPAGE
profiele is Russiese koringluis-weerstandbiedende 1BL.1 RStranslokasie
homosigote ge"ldentifiseer en kon bevestig word dat die
weerstandsgeen vir die Russiese koringluis oorgedra is na die 1BL.1 RS
('Veery') -translokasie.
Twee strategies is gevolg om die Russiese koringluislokus of -loci te karteer:
(1) 'n Telosomiese analise is gedoen. 'n Plant met 2n = 40 + 1BL.1 RS +
1RS is verkry en met 'n luisvatbare koring bestuif. (2) 'n Gerekombineerde, disomiese plant met Russiese koringluis-weerstandbiedendheid maar sonder
die Lr26 en Sr31 allele is gekruis met 'Gamtoos' en die F1 getoetskruis. Die
toetskruisouer in beide die strategiee was 'Chinese Spring'. In die eerste
eksperiment is die Sr31-lokus 10.42 kaarteenhede vanaf die Lr26-lokus
gelokaliseer. Die raesdata het ge"impliseer dat onbetraubare genetiese
kaarteenhede geskat sou word en daarom is die omslagtige Russiese
koringluis weerstandsbepalings nie gedoen nie. In die tweede eksperiment
is die Russiese koringluis-weerstandsgeen op 14.5 kaarteenhede vanaf die
Lr26-lokus gelokaliseer. Nie-Mendeliese segregasie van die Russiese
koringluis-weerstand in hierdie karteringseksperiment het ge'impliseer dat die
berekende kaartafstand onakkuraat mag wees. Dit was ook nie moontlik om
op grand van die data die aantal gene betrakke af te lei nie.
6
Galagedara, Nelomie Nayanathara. "Identification of Quantitative Trait Loci for Resistance to Tan Spot in Durum Wheat." Thesis, North Dakota State University, 2018. https://hdl.handle.net/10365/28765.
Texto completoResumen
Tan spot, caused by Pyrenophora tritici-repentis (Ptr), is a major foliar disease on wheat. The pathosystem involves three pairs of necrotrophic effector (NE) and host sensitivity (S) gene interactions, namely Ptr ToxA-Tsn1, Ptr ToxB-Tsc2 and Ptr ToxC-Tsc1. Additionally, genetic factors conferring race-nonspecific resistance have been identified. The objectives of this study were to map tan spot resistance QTL and investigate the role of NE-S interactions in disease in durum using association and bi-parental mapping. Evaluation of a worldwide collection of durum accessions allowed identifying highly resistant nineteen lines to multiple Ptr races. Association mapping revealed genomic regions on chromosomes 1A, 2B and 3B significantly associated with resistance to tan spot, which likely correspond to Tsc1, Tsc2 and racenonspecific resistance. Using a bi-parental population derived from Ben and PI 41025, we found that ToxA-Tsn1 interaction plays no significant role in disease, instead a major race-nonspecific QTL on chromosome 5A was identified.
7
Njom, Henry Akum. "Mechanism and synchronicity of wheat (Triticum aestivum) resistance to leaf rust (Puccinia triticina) and Russian wheat aphid (Duiraphis noxia) SA1." Thesis, University of Fort Hare, 2016. http://hdl.handle.net/10353/2700.
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Wheat (Triticum aestivum and T. Durum) is an extremely important agronomic crop produced worldwide. Wheat consumption has doubled in the last 30 years with approximately 600 million tons consumed per annum. According to the International Maize and Wheat Improvement Center, worldwide wheat demand will increase over 40 percent by 2020, while land as well as resources available for the production will decrease significantly if the current trend prevails. The wheat industry is challenged with abiotic and biotic stressors that lead to reduction in crop yields. Increase knowledge of wheat’s biochemical constitution and functional biology is of paramount importance to improve wheat so as to meet with this demand. Pesticides and fungicides are being used to control biotic stress imposed by insect pest and fungi pathogens but these chemicals pose a risk to the environment and human health. To this effect, there is re-evaluation of pesticides currently in use by the Environmental Protection Agency, via mandates of the 1996 Food Quality Protection Act and those with higher perceived risks are banned. Genetic resistance is now a more environmental friendly and effective method of controlling insect pest and rust diseases of wheat than the costly spraying with pesticides and fungicides. Although, resistant cultivars effectively prevent current prevailing pathotypes of leaf rust and biotypes of Russian wheat aphid from attacking wheat, new pathotypes and biotypes of the pathogen/pest may develop and infect resistant cultivars. Therefore, breeders are continually searching for new sources of resistance. Proteomic approaches can be utilised to ascertain target enzymes and proteins from resistant lines that could be utilised to augment the natural tolerance of agronomically favourable varieties of wheat. With this ultimate goal in mind, the aim of this study was to elucidate the mechanism and synchronicity of wheat resistance to leaf rust (Puccinia triticina) and Russian wheat aphid (Duiraphis noxia) SA1. To determine the resistance mechanism of the wheat cultivars to leaf rust infection and Russian wheat aphid infestation, a proteomics approach using two-dimensional gel electrophoresis was used in order to determine the effect of RWA SA1 on the wheat cultivars proteome. Differentially expressed proteins that were up or down regulated (appearing or disappearing) were identified using PDQuestTM Basic 2-DE Gel analysis software. Proteins bands of interest were in-gel trypsin digested as per the protocol described in Schevchenko et al. (2007) and analysed using a Dionex Ultimate 3000 RSLC system coupled to an AB Sciex 6600 TripleTOF mass spectrometer. Protein pilot v5 using Paragon search engine (AB Sciex) was used for comparison of the obtained MS/MS spectra with a custom database containing sequences of Puccinia triticina (Uniprot Swissprot), Triticum aestivum (Uniprot TrEMBL) and Russian wheat aphid (Uniprot TrEMBL) as well as a list of sequences from common contaminating proteins. Proteins with a threshold of ≥99.9 percent confidence were reported. A total of 72 proteins were putatively identified from the 37 protein spots excised originating from either leaf rust or Russian wheat aphid experiments. Sixty-three of these proteins were associated with wheat response to stress imposed by RWA SA1 feeding while 39 were associated with infection by Puccinia triticina. Several enzymes involved in the Calvin cycle, electron transport and ATP synthesis were observed to be differentially regulated suggesting greater metabolic requirements in the wheat plants following aphid infestation and leaf rust infection. Proteins directly associated with photosynthesis were also differentially regulated following RWA SA1 infestation and P.
8
Ramburan, Viresh Premraj. "Genetic mapping of adult plant stripe rust resistance in the wheat cultivar Kariega." Thesis, Stellenbosch : Stellenbosch University, 2003. http://hdl.handle.net/10019.1/53438.
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Thesis (PhD (Agric)) -- Stellenbosch University, 2003.<br>ENGLISH ABSTRACT: Stripe (yellow) rust of wheat, caused by Puccinia striiformis f.sp. tritici, was first detected as a
single introduction into South Africa in 1996. Two additional pathotypes have since been
identified. Control of the disease may be achieved by use of genetic adult plant resistance
(APR) as is present in the local cultivar 'Kariega'. The aim of this project was to understand the
genetic basis of the APR in 'Kariega' to facilitate breeding of new varieties with genetic
resistance to stripe rust.
A partial linkage map of a 'Kariega X Avocet S' doubled haploid population covering all 21
wheat chromosomes was generated using 208 DNA markers, viz, 62 SSR, 133 AFLP, 3 RGA
and 10 SRAP markers, and 4 alternative loci. The different marker techniques detected varying
polymorphism, viz, overall SSR: 46%, AFLP: 7%, SRAP: 6% and RGA: 9%, and the markers
produced low levels of missing data (4%) and segregation distortion (5%). A significant feature
of the linkage map was the low polymorphism found in the D genome, viz, 19% of all mapped
DNA markers, 11% of all AFLP markers and 30% of the total genome map distance. A region
exhibiting significant segregation distortion was mapped to chromosome 4A and a seedling
resistance gene for stem rust (Puccinia graminis f.sp . tritici), Sr26, mapped to chromosome 6A
close to three SSR markers. The leaf tip necrosis gene, Ltn, which was also segregating in the
population, mapped to chromosome 7D. Protocols for SRAP and RGA were optimised, and
SRAP marker use in wheat genetic linkage studies is reported for the first time.
The linkage map was used together with growth chamber and replicated field disease scores for
QTL mapping. Chromosomes showing statistically significant QTL effects were then targeted
with supplementary SSR markers for higher resolution mapping. The quality of disease
resistance phenotypic data was confirmed by correlation analysis between the different scorers
for reaction type (0.799±0.023) and for transformed percentage leaf area infected
(0.942±0.007).
Major QTL were consistently identified on chromosome 7D (explaining some 25-48% of the
variation) and on chromosome 2B (21-46%) using transformed percentage leaf area infected and transformed reaction type scores (early and final) with interval mapping and modified
interval mapping techniques. Both chromosomal regions have previously been identified in
other studies and the 7D QTL is thought likely to be the previously mapped APR gene Yr 18.
Minor QTL were identified on chromosomes lA and 4A with the QTL on 4A being more
prominent at the early field scoring for both score types. A QTL evidently originating from
'Avocet S' was detected under growth chamber conditions but was not detected in the field,
suggesting genotype-environment interaction and highlighting the need for modifications of
growth chamber conditions to better simulate conditions in the field.
The genetic basis of the APR to stripe rust exhibited by 'Kariega' was established by mapping
of QTL controlling this trait. The linkage map constructed will be a valuable resource for
future genetic studies and provides a facility for mapping other polymorphic traits in the
parents of this population with a considerable saving in costs.<br>AFRIKAANSE OPSOMMING: Streep of geelroes van koring word veroorsaak deur Puccinia striiformis f. sp tritici, en is die
eerste keer in 1996 in Suid-Afrika na introduksie van 'n enkele patotipe waargeneem. Twee
verdere patotipes is sedertdien in Suid-Afrika gei"dentifiseer. Beheer van die siekte word veral
moontlik gemaak deur die gebruik van genetiese volwasseplantweerstand soos gei"dentifiseer in
die plaaslike kultivar 'Kariega'. Die doel van hierdie studie was om die genetiese grondslag van
die streeproesweerstand te ontrafel ten einde die teling van nuwe bestande kultivars moontlik te
maak.
'n Verdubbelde haplo1ede populasie uit die kruising 'Kariega X Avocet S' is aangewend om 'n
gedeeltelike koppelingskaart vir die volle stel van 21 koring chromosome saam te stel. Die kaart
het uit 208 DNA merkers, nl., 62 SSR, 133 AFLP, 3 RGA, 10 SRAP merkers en 4 ander lokusse
bestaan. Totale polimorfisme wat deur die verskillende merkersisteme opgespoor is, was as volg:
SSR: 46%, RGA: 9%, AFLP: 7% en SRAP: 6%. Die mate van ontbrekende data was gering
(4%) asook die mate van segregasie distorsie (5%) van 'n enkele geval wat op chromosoom 4A
gekarteer is. 'n Prominente kenmerk van die koppelingskaart is die relatiewe gebrek aan
polimorfiese merkers op die D-genoom, nl., slegs 19% van alle DNA merkers en 11% van alle
AFLP merkers wat slegs 30% van die totale genoom kaartafstand bestaan het. Die stamroes
(Puccinia graminis f. sp. tritici) saailingweerstandsgeen, Sr26, karteer op chromosoom 6A naby
drie SSR merkers. Die geen vir blaartipnekrose, Ltn, karteer op chromosoom 7D. Protokolle vir
SRAP en RGA merkers is ge-optimiseer en gebruik van SRAP merkers in koppelings-analise
word vir die eerste keer in koring gerapporteer.
Die koppelingskaart is in kombinasie met groeikamerdata en gerepliseerde veldproefdata gebruik
om die gene (QTL) vir volwasseplant streeproesweerstand te karteer. Chromosome met statisties
betekenisvolle QTL is met aanvullende SSR merkers geteiken om die resolusie van kartering
verder te verhoog. Die kwaliteit van fenotipiese data, soos in die proewe aangeteken, is bevestig
deur korrelasies te bereken tussen lesings geneem deur onafhanklike plantpataloe (0.799 ± 0.023
vir reaksietipe en 0.942 ± 0.007 vir getransformeerde persentasie blaaroppervlakte besmet).
Hoofeffek QTL vir die twee maatstawwe van weerstand is deur middel van die metodes van
interval QTL kartering en gemodifiseerde interval QTL kartering konsekwent op chromosome
7D (25-48% van variasie verklaar) en 2B (21-46% van variasie verklaar) ge"identifiseer. In
vorige studies is aangetoon dat beide chromosome 7D en 2B QTL vir volwasseplant
streeproesweerstand dra. Die 7D QTL is waarskynlik die weerstandsgeen, Yr 18. QTL met klein
effekte op weerstand is op chromosome lA en 4A ge"identifiseer. Die effek van laasgenoemde
geen was meer prominent in die velddata in die vroee datum van weerstandsbeoordeling. Een
QTL, afkomstig van 'Avocet S', is slegs onder groeikamertoestande identifiseerbaar. Dit dui op
moontlike genotipe-omgewing wisselwerking en beklemtoon die noodsaaklikheid om
aanpassings te maak in groeikamertoestande vir beter simulasie van veldproeftoestande.
Die genetiese grondslag van volwasseplantweerstand teen streeproes in die kultivar 'Kariega' is
deur QTL kartering bepaal. Die 'Kariega X Avocet S' koppelingskaart kan as 'n waardevolle basis
dien vir toekomstige genetiese ontledings van ander polimorfiese kenmerke in die populasie.
9
Bierman, Anandi. "Mapping and survey sequencing of Dn resistance genes in Triticum aestivum L." Thesis, Stellenbosch : Stellenbosch University, 2015. http://hdl.handle.net/10019.1/96912.
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Thesis (PhD)--Stellenbosch University, 2015<br>ENGLISH ABSTRACT : Diuraphis noxia Kurdjumov (Russian Wheat Aphid; RWA) is a pest of wheat and barley that has spread from its home range in the fertile crescent to most wheat producing countries except Australia. Since its first introduction to South Africa and the USA in the late 20th century, breeding programs for wheat phenotypes
resistant to the aphid were put in place. Conventional breeding practices rely on phenotypic screening to verify traits carried by offspring and genetic tools such as marker assisted selection (MAS) have greatly aided this process in speed and accuracy. The size and complexity of the wheat genome, its allopolyploid nature and repetitive elements have, however, posed a challenge to studies on the genetics of this cereal crop. Many studies have focused on chromosome 3B which is the largest of the wheat chromosomes and easily separated from the redundant genomic background by techniques such as flow cytometry. The similarity in size of the remaining chromosomes however, limits the application of flow cytometry to their isolation. Databases such as Grain-Genes (http://wheat.pw.usda.gov/GG2/index.shtml) house marker data from various mapping studies for all wheat chromosomes and in 2014 the International Wheat Genome Sequencing Consortium (IWGSC) completed the draft genome sequence of wheat categorized by chromosome. Sources of resistance (Dn resistance genes) against RWA are located on chromosome 7D. but despite the marker and sequence data available currently, mapping studies specific for the Dn resistance genes are few. Additionally, sequence data available is derived from cultivars susceptible to RWA and is not comprehensively annotated and assembled in many cases. In this study, we demonstrate a novel, combined approach to isolate and characterize the Dn resistance genes through the use of a genetic map constructed from Amplified Fragment Length Polymorphism (AFLP), Expressed Sequence Tag (EST) and microsatellite markers and a physical map constructed from Next Generation
Sequencing (NGS) data of ditelosomic chromosomes (7DS and 7DL) isolated by microdissection on the PALM microbeam system. A 122.8 cM genetic map was produced from 38 polymorphic AFLP markers and two ESTs with the microsatellite Xgwm111 as anchor to related genetic maps. Through comparison to maps available on GrainGenes the location of the Dn1 resistance gene was narrowed down to a deletion bin (7DS5-0.36-0.62) on the short arm of chromosome 7D with an AFLP marker (E-ACT/M-CTG_0270.84) mapping closely at 3.5 cM and two ESTs mapping at 15.3 cM and 15.9 cM from Dn1. Isolation of individual chromosome arms 7DS and 7DL using the PALM Microbeam system
allowed sequencing of the chromosome without the redundancy of the remainder
of the hexaploid genome. Through isolating the chromosome arms in this way, a >80-fold reduction in genome size was achieved as well as a major reduction in repetitive elements. Analysis of the sequencing data confirmed that 7DL is the physically shorter arm of the chromosome though it contains the majority of protein coding sequences.<br>AFRIKAANSE OPSOMMING : Diuraphis noxia Kurdjumov (Russiese koring-luis; RWA) is « pes wat op koring en gars voorkom. Die pes het vanaf sy tuiste in die midde Ooste na meeste koringproduserende lande behalwe Australië versprei. Sedert die eerste bekendstelling van RWA in Suid Afrika en die VSA in die vroeë 20ste eeu is teelprogramme
ten gunste van koring lyne met weerstand teen RWA begin. Tradisionele teelprogramme maak op fisieise observasie van die fenotipe staat om te verifieer of plante in die nageslag die gewenste eienskap dra. Genetiese metodes soos merkerondersteunde
seleksie (MAS) versnel hierdie selekteringsproses grootliks. Die grootte en kompleksiteit van die koring genoom asook die polyploïde en herhalende natuur
daarvan is « groot hindernis vir genetiese studies van hierdie graangewas. Baie studies het op chromosoom 3B gefokus wat die grootste van die koring chromosome
is en dus maklik vanaf die res van die oorbodige genomiese agtergond deur tegnieke soos vloeisitometrie geskei word. Die ooreenkoms in grootte tussen die res
van die chromosome bemoeilik die toepassing van vloeisitometrie om hulle te isoleer. Databasisse soos GrainGenes (http://wheat.pw.usda.gov/GG2/index.shtml)
bevat merker data vanaf verskeie karterings-studies vir al die chromosome en in 2014 het die "International Wheat Genome Sequencing Consortium"(IWGSC) die
voorlopige basispaarvolgorde van die koring genoom bekendgestel, gekategoriseer volgens chromosoom. Weerstandsbronne (Dn weerstandsgene) teen RWA kom
meestal op chromosoom 7D voor. Ten spyte van merker en basispaarvolgorde data tans beskikbaar is karterings-studies spesifiek tot die Dn gene skaars en basispaarvolgorde data is vanaf kultivars afkomstig wat nie weerstandbiedend teen RWA is nie en waarvan die annotasie en samestelling baie keer nie goed is nie. In hierdie studie demonstreer ons « nuwe, gekombineerde aanslag om die Dn weerstandsgene te isoleer en karakteriseer deur van « genetiese kaart opgestel met "Amplified Fragment Length Polymorphism"(AFLP), "Expressed Sequence Tag"(EST) en mikrosatelliet
merkers asook « fisiese kaart saamgestel deur die volgende-generasiebasispaarvolgordebepaling
van ditelosomiese chromosome (7DS en 7DL) geïsoleer
deur mikrodisseksie met die "PALM Microbeam"sisteem gebruik te maak. « Genetiese kaart van 122.8 cM was met 38 polimorfiese AFLP merkers en twee EST
merkers geskep. Die mikrosatelliet, Xgwm111, is ook ingesluit en het as anker vir verwante genetiese-kaarte gedien. Deur vergelyking met genetiese-kaarte op
GrainGenes is die posisie van die Dn1 weerstandsgeen vernou na « delesie bin (7DS5-0.36-0.62) op die kort arm van chromosoom 7D met « AFLP merker (EACT/
M-CTG_0270.84) wat ongeveer 3.5 cM vanaf die geen karteer. Die twee EST merkers is 15.3 cM en 15.9 cM vanaf die geen gekarteer. Isolering van die individuele
chromosoom arms, 7DS en 7DL, deur van die "PALM Microbeam"sisteem gebruik te maak het basispaarvolgordebepaling van die chromosoom toegelaat sonder die oortolligheid van die res van die hexaploïde genoom. Deur die chromosoom so te isoleer is « >80-maal verkleining in genoom grootte bereik insluitend « groot reduksie in herhalende elemente. Analise van die data vanaf basispaarvolgordebepaling
het bevestig dat chromosoom 7D die fisiese kleiner chromosoom is maar dat dit die meerderheid van proteïn koderende basispaarvolgordes bevat.
10
Khan, Imtiaz Ahmed. "Utilisation of molecular markers in the selection and characterisation of wheat-alien recombiant chromosomes." Title page, contents and summary only, 1996. http://web4.library.adelaide.edu.au/theses/09PH/09phk451.pdf.
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Bibliography: leaves 137-163. his is a comprehensive study of induced homoeologous recombination along most of the complete genetic length of two homoeologous chromosomes in the Triticeae (7A of common wheat and 7Ai of Agropyron intermedium), using co-dominant DNA markers. Chromosome 7Ai was chosen as a model alien chromosome because is has been reported to carry agronomically important genes conferring resistance to stem rust and barley yellow dwarf virus on its short and long arms, respectively.
Libros sobre el tema "Wheat Disease and pest resistance"
1
Sharma, Indu. Disease resistance in wheat. Wallingford, Oxfordshire, UK: CABI, 2012.
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2
Forsström, Per-Olov. Broadening of mildew resistance in wheat. Alnarp: Swedish University of Agricultural Sciences, 2002.
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4
Stem rust of wheat: From ancient enemy to modern foe. St. Paul, Minn: APS Press, 2001.
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5
Marasas, C. N. The economic impact in developing countries of leaf rust resistance breeding in CIMMYT-related spring bread wheat. México, D.F., México: CIMMYT, 2004.
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6
Horst, Mielke. Untersuchungen über Fusarium culmorum (W.G.Sm.) Sacc. als Fuss- und Ährenkrankheitserreger beim Weizen. Berlin: Kommissionsverlag P. Parey, 1988.
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7
International Wheat Conference (7th Mar del Plata, Argentina 2005). Wheat production in stressed environments: Proceedings of the 7th International Wheat Conference, 27 November - 2 December 2005, Mar del Plata, Argentina. Dordrecht, the Netherlands: Springer, 2007.
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8
Sharma, I., ed. Disease resistance in wheat. Wallingford: CABI, 2012. http://dx.doi.org/10.1079/9781845938185.0000.
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9
1960-, Parker Jane, ed. Molecular aspects of plant disease resistance. Ames, Iowa: Blackwell, 2008.
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10
Rosenthal, Ed. Marijuana pest and disease control. Oakland, CA: Quick American, 2012.
Buscar texto completoCapítulos de libros sobre el tema "Wheat Disease and pest resistance"
1
Ayliffe, Michael, Ming Luo, Justin Faris, and Evans Lagudah. "Disease Resistance." In Wheat Improvement, 341–60. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-90673-3_19.
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AbstractWheat plants are infected by diverse pathogens of economic significance. They include biotrophic pathogens like mildews and rusts that require living plant cells to proliferate. By contrast necrotrophic pathogens that cause diseases such as tan spot, Septoria nodurum blotch and spot blotch require dead or dying cells to acquire nutrients. Pioneering studies in the flax plant-flax rust pathosystem led to the ‘gene-for-gene’ hypothesis which posits that a resistance gene product in the host plant recognizes a corresponding pathogen gene product, resulting in disease resistance. In contrast, necrotrophic wheat pathosystems have an ‘inverse gene-for-gene’ system whereby recognition of a necrotrophic fungal product by a dominant host gene product causes disease susceptibility, and the lack of recognition of this pathogen molecule leads to resistance. More than 300 resistance/susceptibility genes have been identified genetically in wheat and of those cloned the majority encode nucleotide binding, leucine rich repeat immune receptors. Other resistance gene types are also present in wheat, in particular adult plant resistance genes. Advances in mutational genomics and the wheat pan-genome are accelerating causative disease resistance/susceptibility gene discovery. This has enabled multiple disease resistance genes to be engineered as a transgenic gene stack for developing more durable disease resistance in wheat.
2
Mottaleb, Khondoker Abdul. "Impacts of Transboundary Crop Diseases on Sustainable Crop Production: The Case of Maize Lethal Necrosis (MLN) in Africa." In Emerging-Economy State and International Policy Studies, 163–79. Singapore: Springer Nature Singapore, 2022. http://dx.doi.org/10.1007/978-981-19-5542-6_13.
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AbstractMore than half of the world’s population relies on wheat, maize, and rice for their daily dietary energy. In 2019, the daily per person average calorie intake was 2,963 kilocalories (kcal), in which the share was more than 18.2% (538 kcal) for wheat, 5.4% (159 kcal) for maize, and 18.3% (542 kcal) for rice. It is projected that by 2050, the total global population is expected to reach between 8.9 and 10.6 billion from 7.8 billion in 2020. Thus, it will be imperative to produce more wheat, maize, and rice to ensure the food security of the world’s burgeoning population. While it is imperative to produce more food, the emergence and re-emergence of lethal crop diseases and their spread from the epicenters to new regions continuously threaten crop yield, farmers’ income, and the world’s food security. For example, the emergence of maize lethal necrosis (MLN) in Africa has generated a credible threat to global and African food security. This study quantified MLN-induced maize production loss in Kenya, DR Congo, and Tanzania. Applying the time-series projection method, this study estimates that the loss in maize production due to MLN was 442 thousand tons in Kenya, nearly 12 thousand tons in DR Congo, and 663 thousand tons in Tanzania. As more pest- and disease-related crop losses are expected due to the changes in global climate, this study concludes by suggesting that it is imperative to invest more in research and development of disease-resistant crop varieties globally to ensure food and nutrition security, particularly in the global south.
3
Johnson, R., and F. G. H. Lupton. "Breeding for disease resistance." In Wheat Breeding, 369–424. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-3131-2_13.
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Tadesse, Wuletaw, Marion Harris, Leonardo A. Crespo-Herrera, Body Mori, Zakaria Kehel, and Mustapha El Bouhssini. "Insect Resistance." In Wheat Improvement, 361–78. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-90673-3_20.
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AbstractStudies to-date have shown the availability of enough genetic diversity in the wheat genetic resources (land races, wild relatives, cultivars, etc.) for resistance to the most economically important insect pests such as Hessian fly, Russian wheat aphid, greenbug, and Sun pest. Many R genes – including 37 genes for Hessian fly, 11 genes for Russian wheat aphid and 15 genes for greenbug – have been identified from these genetic resources. Some of these genes have been deployed singly or in combination with other genes in the breeding programs to develop high yielding varieties with resistance to insects. Deployment of resistant varieties with other integrated management measures plays key role for the control of wheat insect pests.
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Gupta, A. K., and R. G. Saini. "Leaf Rust Resistance in Wheat." In Durability of Disease Resistance, 235–37. Dordrecht: Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-2004-3_25.
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Zhang, H. S., R. E. Niks, R. G. Dekens, and H. H. Lubbers. "Inheritance of Resistance to Wheat Leaf Rust (Puccinia Recondita) in four Accessions of Diploid Wheat." In Durability of Disease Resistance, 358. Dordrecht: Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-2004-3_83.
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McIntosh, R. A., C. R. Wellings, and R. F. Park. "Wheat Rusts and the Genetic Bases of Disease Resistance." In Wheat Rusts, 1–28. Dordrecht: Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-011-0083-0_1.
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Bartoš, P., E. Stuchlíková, and R. Hanušová. "Durability of Wheat Disease Resistance in Czechoslovakia." In Durability of Disease Resistance, 307. Dordrecht: Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-2004-3_35.
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Van Silfhout, C. H. "Durable Resistance in the Pathosystem: Wheat — Stripe Rust." In Durability of Disease Resistance, 135–45. Dordrecht: Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-2004-3_11.
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Broers, L. H. M. "Breeding for Partial Resistance in Wheat to Stripe Rust." In Durability of Disease Resistance, 179–83. Dordrecht: Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-2004-3_14.
Texto completoActas de conferencias sobre el tema "Wheat Disease and pest resistance"
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"Using synthetic forms of RS5 and RS7 to expand the genetic diversity of common wheat for disease resistance." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Novosibirsk ICG SB RAS 2021, 2021. http://dx.doi.org/10.18699/plantgen2021-036.
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Novikova, I. I., E. V. Popova, L. E. Kolesnikov, and Yu R. Kolesnikova. "Influence of biologicals on photosynthetic pigments in wheat leaves." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.185.
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Multifunctional biological products based on strains of microorganisms that are antagonists of pathogens and plant disease resistance activators - chitosan and its derivatives increase the content of chlorophyll α and b in flag leaves of wheat, the number and weight of grains in the ear, potential yield, and also reduce the development of yellow rust. The maximum biological effectiveness for these indicators was noted in the experimental version, where Bacillus subtilis VKM B-2604D and B. subtilis VKM B-2605D strains that are part of the Vitaplan biological product and chitosan salicylate (Chitosan II) complex was used.
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STRAZDINA, Vija, Valentina FETERE, Liga FEODOROVA-FEDOTOVA, Janis JASKO, and Olga TREIKALE. "REACTION OF WINTER WHEAT GENOTYPES ON THE YELLOW (STRIPE) RUST PUCCINIA STRIIFORMIS, WES." In RURAL DEVELOPMENT. Aleksandras Stulginskis University, 2018. http://dx.doi.org/10.15544/rd.2017.124.
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Yellow rust, caused by Puccinia striiformis Wes. is one of the most significant diseases constraint to winter wheat production in the world. Since 2011 in Europe have appeared distinct new races – Warrior, Kranich, Warrior (-) that have caused wide epidemics on different cultivars of wheat. Grain yield losses can be prevented by using a combination of varietal resistance and fungicides. Information on wheat variety susceptibility to local yellow (stripe) rust Puccinia striiformis Wes. races can help to reduce the risk of yield losses in high disease pressure situations. Field trials with eight most popular and perspective winter wheat varieties in Latvia were established in the North-Western part of Latvia (Stende Research Centre) in autumn of 2016. The trial was designed as two randomized complete blocks (treated and untreated) and data were statistically interpreted. Two applications of fungicides at BBCH 29-32 by T1 (prothioconazol 53 g L-1, spiroxamin 224 g L-1, tebucanazole 148 g L-1) and at BBCH 37-39 - T2 (bixafen 65 g L-1, prothioconazol 130 g L-1, fluopyram 65 g L-1- 1.5 L ha-1) were used to control the YR. Yield and 1000 kernel weight (TKW) were determined. Preliminary results indicated the difference between genotypes resistance/susceptibility to YR. The severity of infection level was 1- 80% depending on genotype resistance. Application of fungicides increased grain yield by 2.9 % to 33.0% and TKW by 3.4% - 33.2 % depending on variety. Observations showed the difference in the occurrence of symptoms on YR in different varieties of winter wheat under conditions of 2017 in Stende.
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Sasco, Elena. "Efectele genetice implicate în răspunsul grăului comun la filtratul de cultură Drechslera sorokiniana (SACC.) subram." In VIIth International Scientific Conference “Genetics, Physiology and Plant Breeding”. Institute of Genetics, Physiology and Plant Protection, Republic of Moldova, 2021. http://dx.doi.org/10.53040/gppb7.2021.71.
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Helminthosporiosis caused by the fungus Drechslera sorokiniana (Sacc.) causes significant crop and quality losses to Triticum aestivum L. in agroecological conditions with extreme humidity. Increasing the resistance is considered the most cost-effective and sustainable approach to disease control. The aim of this study was to determine the genetic effects involved in the inheritance of resistance, using the ge-netic model of character reproduction in descendants of wheat. Generations F1, F2, BCP1 and BCP2, de-scended from the mutual crossing of the parents Basarabeanca / Moldova 30 and Moldova 30 / Moldova 3 (P1 and P2) were evaluated for the response of callus characters to the action of D. sorokiniana culture filtrate on the medium Murashige Skoog. Fungal metabolites have decreased the effects of gene actions and epistatic interactions, but also their variance. The phenomenon corresponds to the decrease of callus indices. A great importance for the heredity of the character of the surface of the callus manifested the epistatic effects of additive-dominant (ad) type. In the case of callus biomass comparable to the mean val-ues were the a actions, but also the ad and dd epistatic effects. The predominant involvement of epistatic effects indicates the need for resistance selections to helminthosporiosis in late generations of wheat.
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Eynck, Christina. "Camelina breeding and development- a Canadian perspective." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/bsmv8815.
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Climate change is predicted to have a more profound impact on the Canadian Prairies compared to other regions in the world, with higher relative temperatures, longer periods of water stress and increased frequency of droughts. Camelina sativa (camelina) is a promising alternative, climate-resilient oilseed that could become part of a Canadian strategy to battle climate change and its detrimental effects on agriculture. Albeit currently a small crop, camelina has enormous potential for growth: favorable agronomics, like early maturity, frost and drought tolerance, pest and disease resistance, as well as exceptional winter hardiness in true winter types in combination with a unique oil profile render it an excellent feedstock crop not only for biofuel, but also high value feed and food uses. Uses for camelina oil and meal include industrial applications (e.g. biodiesel, lubricants, and polymers) and higher value areas such as cosmetics, Omega-3 supplements for human and companion animal nutrition, and applications in the livestock, poultry and aquaculture feed sectors. As a relatively undeveloped crop, there is significant potential for improvement of both agronomic and seed quality characteristics. This presentation will provide an overview of current camelina breeding and crop development efforts underway at the AAFC Research and Development Center in Saskatoon in collaboration with industry. This includes variety and germplasm development in spring- and winter-type camelina, insights into the genomics of camelina as well as recent developments in the Canadian camelina industry.
Informes sobre el tema "Wheat Disease and pest resistance"
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Sela, Hanan, Eduard Akhunov, and Brian J. Steffenson. Population genomics, linkage disequilibrium and association mapping of stripe rust resistance genes in wild emmer wheat, Triticum turgidum ssp. dicoccoides. United States Department of Agriculture, January 2014. http://dx.doi.org/10.32747/2014.7598170.bard.
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The primary goals of this project were: (1) development of a genetically characterized association panel of wild emmer for high resolution analysis of the genetic basis of complex traits; (2) characterization and mapping of genes and QTL for seedling and adult plant resistance to stripe rust in wild emmer populations; (3) characterization of LD patterns along wild emmer chromosomes; (4) elucidation of the multi-locus genetic structure of wild emmer populations and its correlation with geo-climatic variables at the collection sites. Introduction In recent years, Stripe (yellow) rust (Yr) caused by Pucciniastriiformis f. sp. tritici(PST) has become a major threat to wheat crops in many parts of the world. New races have overcome most of the known resistances. It is essential, therefore, that the search for new genes will continue, followed by their mapping by molecular markers and introgression into the elite varieties by marker-assisted selection (MAS). The reservoir of genes for disease and pest resistance in wild emmer wheat (Triticumdicoccoides) is an important resource that must be made available to wheat breeders. The majority of resistance genes that were introgressed so far in cultivated wheat are resistance (R) genes. These genes, though confering near-immunity from the seedling stage, are often overcome by the pathogen in a short period after being deployed over vast production areas. On the other hand, adult-plant resistance (APR) is usually more durable since it is, in many cases, polygenic and confers partial resistance that may put less selective pressure on the pathogen. In this project, we have screened a collection of 480 wild emmer accessions originating from Israel for APR and seedling resistance to PST. Seedling resistance was tested against one Israeli and 3 North American PST isolates. APR was tested on accessions that did not have seedling resistance. The APR screen was conducted in two fields in Israel and in one field in the USA over 3 years for a total of 11 replicates. We have found about 20 accessions that have moderate stripe rust APR with infection type (IT<5), and about 20 additional accessions that have novel seedling resistance (IT<3). We have genotyped the collection using genotyping by sequencing (GBS) and the 90K SNP chip array. GBS yielded a total 341K SNP that were filtered to 150K informative SNP. The 90K assay resulted in 11K informative SNP. We have conducted a genome-wide association scan (GWAS) and found one significant locus on 6BL ( -log p >5). Two novel loci were found for seedling resistance. Further investigation of the 6BL locus and the effect of Yr36 showed that the 6BL locus and the Yr36 have additive effect and that the presence of favorable alleles of both loci results in reduction of 2 grades in the IT score. To identify alleles conferring adaption to extreme climatic conditions, we have associated the patterns of genomic variation in wild emmer with historic climate data from the accessions’ collection sites. The analysis of population stratification revealed four genetically distinct groups of wild emmer accessions coinciding with their geographic distribution. Partitioning of genomic variance showed that geographic location and climate together explain 43% of SNPs among emmer accessions with 19% of SNPs affected by climatic factors. The top three bioclimatic factors driving SNP distribution were temperature seasonality, precipitation seasonality, and isothermality. Association mapping approaches revealed 57 SNPs associated with these bio-climatic variables. Out of 21 unique genomic regions controlling heading date variation, 10 (~50%) overlapped with SNPs showing significant association with at least one of the three bioclimatic variables. This result suggests that a substantial part of the genomic variation associated with local adaptation in wild emmer is driven by selection acting on loci regulating flowering. Conclusions: Wild emmer can serve as a good source for novel APR and seedling R genes for stripe rust resistance. APR for stripe rust is a complex trait conferred by several loci that may have an additive effect. GWAS is feasible in the wild emmer population, however, its detection power is limited. A panel of wild emmer tagged with more than 150K SNP is available for further GWAS of important traits. The insights gained by the bioclimatic-gentic associations should be taken into consideration when planning conservation strategies.
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Breiman, Adina, Jan Dvorak, Abraham Korol, and Eduard Akhunov. Population Genomics and Association Mapping of Disease Resistance Genes in Israeli Populations of Wild Relatives of Wheat, Triticum dicoccoides and Aegilops speltoides. United States Department of Agriculture, December 2011. http://dx.doi.org/10.32747/2011.7697121.bard.
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Wheat is the most widely grown crop on earth, together with rice it is second to maize in total global tonnage. One of the emerging threats to wheat is stripe (yellow) rust, especially in North Africa, West and Central Asia and North America. The most efficient way to control plant diseases is to introduce disease resistant genes. However, the pathogens can overcome rapidly the effectiveness of these genes when they are wildly used. Therefore, there is a constant need to find new resistance genes to replace the non-effective genes. The resistance gene pool in the cultivated wheat is depleted and there is a need to find new genes in the wild relative of wheat. Wild emmer (Triticum dicoccoides) the progenitor of the cultivated wheat can serve as valuable gene pool for breeding for disease resistance. Transferring of novel genes into elite cultivars is highly facilitated by the availability of information of their chromosomal location. Therefore, our goals in this study was to find stripe rust resistant and susceptible genotypes in Israeli T. dicoccoides population, genotype them using state of the art genotyping methods and to find association between genetic markers and stripe rust resistance. We have screened 129 accessions from our collection of wild emmer wheat for resistance to three isolates of stripe rust. About 30% of the accessions were resistant to one or more isolates, 50% susceptible, and the rest displayed intermediate response. The accessions were genotyped with Illumina'sInfinium assay which consists of 9K single nucleotide polymorphism (SNP) markers. About 13% (1179) of the SNPs were polymorphic in the wild emmer population. Cluster analysis based on SNP diversity has shown that there are two main groups in the wild population. A big cluster probably belongs to the Horanum ssp. and a small cluster of the Judaicum ssp. In order to avoid population structure bias, the Judaicum spp. was removed from the association analysis. In the remaining group of genotypes, linkage disequilibrium (LD) measured along the chromosomes decayed rapidly within one centimorgan. This is the first time when such analysis is conducted on a genome wide level in wild emmer. Such a rapid decay in LD level, quite unexpected for a selfer, was not observed in cultivated wheat collection. It indicates that wild emmer populations are highly suitable for association studies yielding a better resolution than association studies in cultivated wheat or genetic mapping in bi-parental populations. Significant association was found between an SNP marker located in the distal region of chromosome arm 1BL and resistance to one of the isolates. This region is not known in the literature to bear a stripe rust resistance gene. Therefore, there may be a new stripe rust resistance gene in this locus. With the current fast increase of wheat genome sequence data, genome wide association analysis becomes a feasible task and efficient strategy for searching novel genes in wild emmer wheat. In this study, we have shown that the wild emmer gene pool is a valuable source for new stripe rust resistance genes that can protect the cultivated wheat.
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Fahima, Tzion, and Jorge Dubcovsky. Map-based cloning of the novel stripe rust resistance gene YrG303 and its use to engineer 1B chromosome with multiple beneficial traits. United States Department of Agriculture, January 2013. http://dx.doi.org/10.32747/2013.7598147.bard.
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Research problem: Bread wheat (Triticumaestivum) provides approximately 20% of the calories and proteins consumed by humankind. As the world population continues to increase, it is necessary to improve wheat yields, increase grain quality, and minimize the losses produced by biotic and abiotic stresses. Stripe rust, caused by Pucciniastriiformisf. sp. tritici(Pst), is one of the most destructive diseases of wheat. The new pathogen races are more virulent and aggressive than previous ones and have produced large economic losses. A rich source for stripe-rust resistance genes (Yr) was found in wild emmer wheat populations from Israel. Original Project goals: Our long term goal is to identify, map, clone, characterize and deploy in breeding, novel wild emmer Yr genes, and combine them with multiple beneficial traits. The current study was aiming to map and clone YrG303 and Yr15, located on chromosome 1BS and combine them with drought resistance and grain quality genes. Positional cloning of YrG303/Yr15: Fine mapping of these genes revealed that YrG303 is actually allelic to Yr15. Fine genetic mapping using large segregating populations resulted in reduction of the genetic interval spanning Yr15 to less than 0.1 cM. Physical mapping of the YrG303/Yr15 locus was based on the complete chromosome 1BS physical map of wheat constructed by our group. Screening of 1BS BAC library with Yr15 markers revealed a long BAC scaffold covering the target region. The screening of T. dicoccoidesaccession-specific BAC library with Yr15 markers resulted in direct landing on the target site. Sequencing of T. dicoccoidesBAC clones that cover the YrG303/Yr15 locus revealed a single candidate gene (CG) with conserved domains that may indicate a role in disease resistance response. Validation of the CG was carried out using EMS mutagenesis (loss-of- function approach). Sequencing of the CG in susceptible yr15/yrG303 plants revealed three independent mutants that harbour non-functional yr15/yrG303 alleles within the CG conserved domains, and therefore validated its function as a Pstresistance gene. Evaluation of marker-assisted-selection (MAS) for Yr15. Introgressions of Yr15 into cultivated wheat are widely used now. Recently, we have shown that DNA markers linked to Yr15 can be used as efficient tools for introgression of Yr15 into cultivated wheat via MAS. The developed markers were consistent and polymorphic in all 34 tested introgressions and are the most recommended markers for the introgression of Yr15. These markers will facilitate simultaneous selection for multiple Yr genes and help to avoid escapees during the selection process. Engineering of improved chromosome 1BS that harbors multiple beneficial traits. We have implemented the knowledge and genetic resources accumulated in this project for the engineering of 1B "super-chromosome" that harbors multiple beneficial traits. We completed the generation of a chromosome including the rye 1RS distal segment associated with improved drought tolerance with the Yr gene, Yr15, and the strong gluten allele 7Bx-over-expressor (7Bxᴼᴱ). We have completed the introgression of this improved chromosome into our recently released variety Patwin-515HP and our rain fed variety Kern, as well as to our top breeding lines UC1767 and UC1745. Elucidating the mechanism of resistance exhibited by Yr36 (WKS1). The WHEAT KINASE START1 (WKS1) resistance gene (Yr36) confers partial resistance to Pst. We have shown that wheat plants transformed with WKS1 transcript are resistant to Pst. WKS1 is targeted to the chloroplast where it phosphorylates the thylakoid-associatedascorbateperoxidase (tAPX) and reduces its ability to detoxify peroxides. Based on these results, we propose that the phosphorylation of tAPX by WKS1 reduces the ability of the cells to detoxify ROS and contributes to cell death. Distribution and diversity of WKS in wild emmer populations. We have shown that WKS1 is present only in the southern distribution range of wild emmer in the Fertile Crescent. Sequence analysis revealed a high level of WKS1 conservation among wild emmer populations, in contrast to the high level of diversity observed in NB-LRR genes. This phenomenon shed some light on the evolution of genes that confer partial resistance to Pst. Three new WKS1 haplotypes displayed a resistance response, suggesting that they can be useful to improve wheat resistance to Pst. In summary, we have improved our understanding of cereals’ resistance mechanisms to rusts and we have used that knowledge to develop improved wheat varieties.
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Crowley, David E., Dror Minz, and Yitzhak Hadar. Shaping Plant Beneficial Rhizosphere Communities. United States Department of Agriculture, July 2013. http://dx.doi.org/10.32747/2013.7594387.bard.
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PGPR bacteria include taxonomically diverse bacterial species that function for improving plant mineral nutrition, stress tolerance, and disease suppression. A number of PGPR are being developed and commercialized as soil and seed inoculants, but to date, their interactions with resident bacterial populations are still poorly understood, and-almost nothing is known about the effects of soil management practices on their population size and activities. To this end, the original objectives of this research project were: 1) To examine microbial community interactions with plant-growth-promoting rhizobacteria (PGPR) and their plant hosts. 2) To explore the factors that affect PGPR population size and activity on plant root surfaces. In our original proposal, we initially prqposed the use oflow-resolution methods mainly involving the use of PCR-DGGE and PLFA profiles of community structure. However, early in the project we recognized that the methods for studying soil microbial communities were undergoing an exponential leap forward to much more high resolution methods using high-throughput sequencing. The application of these methods for studies on rhizosphere ecology thus became a central theme in these research project. Other related research by the US team focused on identifying PGPR bacterial strains and examining their effective population si~es that are required to enhance plant growth and on developing a simulation model that examines the process of root colonization. As summarized in the following report, we characterized the rhizosphere microbiome of four host plant species to determine the impact of the host (host signature effect) on resident versus active communities. Results of our studies showed a distinct plant host specific signature among wheat, maize, tomato and cucumber, based on the following three parameters: (I) each plant promoted the activity of a unique suite of soil bacterial populations; (2) significant variations were observed in the number and the degree of dominance of active populations; and (3)the level of contribution of active (rRNA-based) populations to the resident (DNA-based) community profiles. In the rhizoplane of all four plants a significant reduction of diversity was observed, relative to the bulk soil. Moreover, an increase in DNA-RNA correspondence indicated higher representation of active bacterial populations in the residing rhizoplane community. This research demonstrates that the host plant determines the bacterial community composition in its immediate vicinity, especially with respect to the active populations. Based on the studies from the US team, we suggest that the effective population size PGPR should be maintained at approximately 105 cells per gram of rhizosphere soil in the zone of elongation to obtain plant growth promotion effects, but emphasize that it is critical to also consider differences in the activity based on DNA-RNA correspondence. The results ofthis research provide fundamental new insight into the composition ofthe bacterial communities associated with plant roots, and the factors that affect their abundance and activity on root surfaces. Virtually all PGPR are multifunctional and may be expected to have diverse levels of activity with respect to production of plant growth hormones (regulation of root growth and architecture), suppression of stress ethylene (increased tolerance to drought and salinity), production of siderophores and antibiotics (disease suppression), and solubilization of phosphorus. The application of transcriptome methods pioneered in our research will ultimately lead to better understanding of how management practices such as use of compost and soil inoculants can be used to improve plant yields, stress tolerance, and disease resistance. As we look to the future, the use of metagenomic techniques combined with quantitative methods including microarrays, and quantitative peR methods that target specific genes should allow us to better classify, monitor, and manage the plant rhizosphere to improve crop yields in agricultural ecosystems. In addition, expression of several genes in rhizospheres of both cucumber and whet roots were identified, including mostly housekeeping genes. Denitrification, chemotaxis and motility genes were preferentially expressed in wheat while in cucumber roots bacterial genes involved in catalase, a large set of polysaccharide degradation and assimilatory sulfate reduction genes were preferentially expressed.
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Steffens, John C., and Eithan Harel. Polyphenol Oxidases- Expression, Assembly and Function. United States Department of Agriculture, January 1995. http://dx.doi.org/10.32747/1995.7571358.bard.
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Polyphenol oxidases (PPOs) participate in the preparation of many plant products on the one hand and cause considerable losses during processing of plant products on the other hand. However, the physiological functions of plant PPO were still a subject of controversy at the onset of the project. Preliminary observations that suggested involvement of PPOs in resistance to herbivores and pathogens held great promise for application in agriculture but required elucidation of PPO's function if modulation of PPO expression is to be considered for improving plant protection or storage and processing of plant products. Suggestions on a possible role of PPO in various aspects of chloroplast metabolism were also relevant in this context. The characterization of plant PPO genes opened a way for achieving these goals. We reasoned that "understanding PPO targeting and routing, designing ways to manipulate its expression and assessing the effects of such modifications will enable determination of the true properties of the enzyme and open the way for controlling its activity". The objective of the project was to "obtain an insight into the function and biological significance of PPOs" by examining possible function(s) of PPO in photosynthesis and plant-pest interactions using transgenic tomato plants; extending our understanding of PPO routing and assembly and the mechanism of its thylakoid translocation; preparing recombinant PPOs for use in import studies, determination of the genuine properties of PPOs and understanding its assembly and determining the effect of PPO's absence on chloroplast performance. Results obtained during work on the project made it necessary to abandon some minor objectives and devote the effort to more promising topics. Such changes are mentioned in the 'Body of the report' which is arranged according to the objectives of the original proposal. The complex expression pattern of tomato PPO gene family was determined. Individual members of the family are differentially expressed in various parts of the plant and subjected to developmentally regulated turnover. Some members are differentially regulated also by pathogens, wounding and chemical wound signals. Wounding systemically induces PPO activity and level in potato. Only tissues that are developmentally competent to express PPO are capable of responding to the systemic wounding signal by increased accumulation of PPO mRNA. Down regulation of PPO genes causes hyper susceptibility to leaf pathogens in tomato while over expression regulation of PPO expression in tomato plants is their apparent increased tolerance to drought. Both the enhanced disease resistance conferred by PPO over expression and the increased stress tolerance due to down regulation can be used in the engineering of improved crop plants. Photosynthesis rate and variable fluorescence measurements in wild type, and PPO-null and over expressing transgenic tomato lines suggest that PPO does not enable plants to cope better with stressful high light intensities or reactive oxygen species. Rather high levels of the enzyme aggravate the damage caused under such conditions. Our work suggests that PPO's primary role is in defending plants against pathogens and herbivores. Jasmonate and ethylene, and apparently also salicylate, signals involved in responses to wounding and defense against herbivores and pathogens, enhance markedly and specifically the competence of chloroplasts to import and process pPPO. The interaction of the precursor with thylakoid membranes is primarily affected. The routing of PPO shows other unusual properties: stromal processing occurs in two sites, resulting in intermediates that are translocated across thylakoids by two different mechanisms - a DpH- and a Sec-dependent one. It is suggested that the dual pattern of processing and routing constitutes a'fail safe' mechanism, reflecting the need for a rapid and flexible response to defense challenges. Many of the observations described above should be taken into consideration when manipulation of PPO expression is contemplated for use in crop improvement.