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Tesi sul tema "Proteins – Biotechnology"

Autore: Grafiati
Pubblicato: 4 giugno 2021
Ultima modifica: 19 febbraio 2022

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1

Schroeder, Michael, Annalisa Marsico, Andreas Henschel, Christof Winter, Anne Tuukkanen, Boris Vassilev та Kerstin Scheubert. "Structural fragment clustering reveals novel structural and functional motifs in α-helical transmembrane proteins". Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-177368.

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Background A large proportion of an organism's genome encodes for membrane proteins. Membrane proteins are important for many cellular processes, and several diseases can be linked to mutations in them. With the tremendous growth of sequence data, there is an increasing need to reliably identify membrane proteins from sequence, to functionally annotate them, and to correctly predict their topology. Results We introduce a technique called structural fragment clustering, which learns sequential motifs from 3D structural fragments. From over 500,000 fragments, we obtain 213 statistically signifi
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2

Ndabambi, Nonkululeko. "Recombinant expression of the pRb- and p53-interacting domains from the human RBBP6 protein for in vitro binding studies." Thesis, University of the Western Cape, 2004. http://etd.uwc.ac.za/index.php?module=etd&amp.

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The aim of this thesis was to produce DNA expression constructs and use them to investigate the feasibility of recombinantly expression proteins for future interaction studies between human RBBP6 and p53 and pRb proteins.
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3

Measey, Thomas J. Schweitzer-Stenner Reinhard. "Unfolded, misfolded, and self-organized short alanine-rich peptides : implications for fundamental science, human disease, and biotechnology /." Philadelphia, Pa. : Drexel University, 2010. http://hdl.handle.net/1860/3317.

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4

Schroeder, Michael, Annalisa Marsico, Andreas Henschel, Christof Winter, Anne Tuukkanen, Boris Vassilev та Kerstin Scheubert. "Structural fragment clustering reveals novel structural and functional motifs in α-helical transmembrane proteins". BioMed Central, 2010. https://tud.qucosa.de/id/qucosa%3A28887.

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Abstract (sommario):
Background A large proportion of an organism's genome encodes for membrane proteins. Membrane proteins are important for many cellular processes, and several diseases can be linked to mutations in them. With the tremendous growth of sequence data, there is an increasing need to reliably identify membrane proteins from sequence, to functionally annotate them, and to correctly predict their topology. Results We introduce a technique called structural fragment clustering, which learns sequential motifs from 3D structural fragments. From over 500,000 fragments, we obtain 213 statistically signifi
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5

Kim, Daniel. "Characterization of the Mata pre-." Scholarly Commons, 2009. https://scholarlycommons.pacific.edu/uop_etds/738.

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6

Sutherland, George A. "De novo designed proteins for applications in research and biotechnology." Thesis, University of Sheffield, 2019. http://etheses.whiterose.ac.uk/22719/.

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While the advances of the scientific community have enabled extraordinary improvements in the capabilities of synthetic biology, there is a continued desire in biotechnology for enhanced or entirely novel biological functions. As proteins are either directly or indirectly responsible for the vast majority of naturally occurring biological activities, the modification of peptide structures constitutes a promising approach to address the ambitions of biotechnology. Central to the work in this thesis is the recognition that naturally occurring protein structures are intangibly complex due to the
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7

Lu, Mason. "Serological analysis and possible exploitation of AID/APOBEC proteins in biotechnology." Thesis, University of Cambridge, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.612091.

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8

Kim, Daniel. "Characterization of the MATα pre-/pro- peptide by mutagenesis as a means to optimize secretion in pichia pistoris". Scholarly Commons, 2009. https://scholarlycommons.pacific.edu/uop_etds/738.

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The unicellular yeast, Pichia pastoris has currently emerged as one of the most popular host systems for heterologous proteins due to its relatively cheap cost, easy genetic manipulability, ability to perform post-translational modifications on proteins, and respiratory growth capabilities which allow it to be cultured in very high concentrations. Over 700 foreign proteins have been recombinantly expressed using P. pastoris. Although P. pastoris appears to be an ideal host system, its main drawback is its inability to efficiently export some heterologous proteins into the extracellular medium.
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9

Huang, Edwin P. C. Biotechnology &amp Biomolecular Sciences Faculty of Science UNSW. "Recombinant protein production utilising a metallothionein expression system and a Super-CHO cell line." Awarded by:University of New South Wales. School of Biotechnology and Biomolecular Sciences, 2006. http://handle.unsw.edu.au/1959.4/24940.

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A novel metal-inducible and amplifiable metallothionein (MT) expression system, pNK, was firstly optimised and characterised for the production of a reporter protein, human growth hormone (hGH) in a suspension CHO cell line grown in a serum-free media. The pNK-based hGH production was demonstrated in cadmium-free condition under various fermentation modes (batch, fed-batch and perfusion) and scales (flask to bench-top bioreactor). Improvement of specific productivity of recombinant protein from pNK was shown to be possible by addition of butyrate or substrate substitution of glutamine by gluta
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10

Landry, David Michael. "Proteins in High Electric Fields." BYU ScholarsArchive, 2013. https://scholarsarchive.byu.edu/etd/4286.

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Being able to control protein function directly and in real time is attractive. In this thesis, I discuss controlling protein activity using alternating current electric fields on the order of 1 MV/m. Since protein structures result in localized and/or protein-wide charge gradients, it is expected that a sufficiently high electric field applied across a protein should result in structural distortions which can temporarily alter or halt protein function. The field is set to oscillate above the influences of the electrochemical double layer effects (1 kHz) and below the level needed for hydrodyn
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11

Baktula, Avinash M. "A Method Based on Conserved Multiple Amino Acid Properties to Predict Amino Acid Substitutions Which Maintain the Protein Structure." TopSCHOLAR®, 2004. http://digitalcommons.wku.edu/theses/1107.

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A METHOD BASED ON CONSERVED MULTIPLE AMINO ACID PROPERTIES TO PREDICT AMINO ACID SUBSTITUTIONS WHICH MAINTAIN THE PROTEIN STRUCTURE Avinash M. Baktula September 16, 2004 1-117 Directed by: Claire A. Rinehart, Doug McElroy and Sigrid Jacobshagen Department of Biology Western Kentucky University Proteins often contain several domains, each with a distinct structure. Such domains have evolved as units that, when combined in various arrangements, produce proteins of unique structure. This study was conducted to identify amino acid substitutions that don’t change structure. Amino acid properties wh
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12

Xu, Qilong. "Isolation and characterization of second protein L-ISOASPARTATE METHYLTRANSFERASE gene in Arabidopsis thaliana." Lexington, Ky. : [University of Kentucky Libraries], 2004. http://lib.uky.edu/ETD/ukyplph2004d00428/XUDISS04.pdf.

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Thesis (Ph. D.)--University of Kentucky, 2004.<br>Title from document title page (viewed on June 22, 2006). Document formatted into pages; contains viii, 116 p. : ill. (some col.). Includes abstract and vita. Includes bibliographical references (p. 103-114).
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13

Corrocher, Flávia Adolfo [UNESP]. "Caracterização funcional de fatores de transcrição hipotéticos de Neurospora crassa." Universidade Estadual Paulista (UNESP), 2012. http://hdl.handle.net/11449/88005.

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Made available in DSpace on 2014-06-11T19:23:05Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-01-16Bitstream added on 2014-06-13T19:29:00Z : No. of bitstreams: 1 corrocher_fa_me_araiq.pdf: 1976492 bytes, checksum: 9e6bb08376e3b4ea3c3817e9d3fe792a (MD5)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)<br>O fungo Neurospora crassa é um organismo amplamente utilizado como organismo modelo na compreensão de aspectos fundamentais da biologia dos eucariotos. Nosso laboratório tem utilizado este fungo pa
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14

Winchester, Christopher Charles. "The roles of Hsp70 proteins in antigen processing and presentation." Thesis, University of Oxford, 1997. http://ora.ox.ac.uk/objects/uuid:567dff45-08ce-43b4-b011-d08afea42f76.

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The ability of members of the hsp70 family to bind to peptides in vivo and in vitro suggests that they may be involved in the processing of antigens for binding to Major Histocompatibility (MHC) class I and/or class n molecules. The aims of this thesis have been to provide evidence for the involvement of hsp70s in antigen processing and to characterise the binding of peptides by hspTOs by structural and functional studies. Firstly, the peptide-binding domains of two hsp70s, hsp70hom and PBP74, were expressed in isolation from the rest of the molecule for structure determination. Both of these
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15

Hultmann, Lisbeth. "Endogenous proteolytic enzymes - Studies of their impact on fish muscle proteins and texture." Doctoral thesis, Norwegian University of Science and Technology, Department of Biotechnology, 2004. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-178.

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<p>This thesis covers studies on endogenous proteolytic enzymes and their impact on fish muscle proteins and texture. The studies have been performed using Atlantic salmon (<i>Salmo salar</i>) and cod (<i>Gadus morhua</i>) subjected to different treatments and storage conditions.</p><p>The textural properties were very different in the two species. Salmon fillets were significantly softer and less resilient than cod fillets, and the properties changed somewhat differently during storage experiments. Different proteolytic enzymes have been reported to participate in muscle softening. Some of th
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16

Kurt, Lutfiye. "Partial Removal Of Proteins From Lactic Acid Fermentation Broth And Recovery Of Proteins From Brewery Wastes By Foam Fractionation Technique." Master's thesis, METU, 2006. http://etd.lib.metu.edu.tr/upload/12607749/index.pdf.

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Foam separation is a simple and economic method for separation of surface-active molecules such as proteins and enzymes from aqueous solutions. In this study, lactic acid broth, spent brewer&rsquo<br>s yeast extract and residual beer was used to investigate the applicability and efficiency of foam separation technique in partial purification of fermentation products and recovery of valuable components from industrial waste streams. The effects of the process variables initial feed concentration, air flow rate, foaming time, liquid pool height and temperature on separation performance were st
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17

Adams, Vicki 1976. "Functional analysis of the clostridial large resolvase TnpX." Monash University, Dept. of Microbiology, 2003. http://arrow.monash.edu.au/hdl/1959.1/5729.

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18

Lang, Claus. "Magnetosome-specific expression of chimeric proteins in Magnetospirillum gryphiswaldense for applications in cell biology and biotechnology." Diss., lmu, 2009. http://nbn-resolving.de/urn:nbn:de:bvb:19-105376.

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19

Kriel, Johan Hendrik. "Development of synthetic signal sequences for heterologous protein secretion from Saccharomyces cerevisiae." Thesis, Stellenbosch : Stellenbosch University, 2003. http://hdl.handle.net/10019.1/53364.

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Thesis (MSc)--Stellenbosch University, 2003.<br>ENGLISH ABSTRACT: Protein secretion and intracellular transport are highly regulated processes and involve the interplay of a multitude of proteins. A unique collection of thermosensitive secretory mutants allowed scientists to demonstrate that the secretory pathway of the yeast Saccharomyces cerevisiae is very similar to that of the higher eukaryotes. All proteins commence their journey in the endoplasmic reticulum, where they undergo amino-linked core glycosyl modification. After passage through the Golgi apparatus, where the remodelling
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20

Yin, Zhao. "Characterization of the biological function of AtEXO70E2." HKBU Institutional Repository, 2018. https://repository.hkbu.edu.hk/etd_oa/483.

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Exocyst positive organelle (EXPO) is a newly discovered double membrane organelle involved in exocytosis and likely other vesicle trafficking processes. EXPO is likely generated from the ER, fused with plasma membrane and released a single membrane vesicle to cell exterior. The Arabidopsis protein Exo70E2 was found to be associated with EXPO and therefore is considered as a marker of EXPO and might play a role in EXPO-mediated vesicle trafficking. Understanding the biological function of AtExo70E2 (abbreviated as E2 in this thesis) will be very helpful in unraveling the function of EXPO. The a
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21

Njunge, James Mwangi. "Characterization of the Hsp40 partner proteins of Plasmodium falciparum Hsp70." Thesis, Rhodes University, 2014. http://hdl.handle.net/10962/d1013186.

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Human malaria is an economically important disease caused by single-celled parasites of the Plasmodium genus whose biology displays great evolutionary adaptation to both its mammalian host and transmitting vectors. This thesis details the 70 kDa heat shock protein (Hsp70) and J protein chaperone complements in malaria parasites affecting humans, primates and rodents. Heat shock proteins comprise a family of evolutionary conserved and structurally related proteins that play a crucial role in maintaining the structural integrity of proteins during normal and stress conditions. They are considere
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22

Ongey, E. (Elvis). "Incorporation of non-canoncical amino acids into recombinant human proteins heterologously expressed in E. coli by bioprocess parturbations." Master's thesis, University of Oulu, 2014. http://urn.fi/URN:NBN:fi:oulu-201406121759.

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The purity of heterologous recombinant proteins is of utmost importance to the pharmaceutical sector since most are consumed as therapeutic agents by humans. Variability caused by co- and posttranslational modifications is a major concern in pharmaceutical production. In order to develop strategies which guarantee a homogeneous product in a robust production process, it is important to better understand the metabolic basis of the synthesis of related non-canonical amino acids. So far, studies have identified high glucose fluxes in connection to oxygen limitation and overexpression of leucine-r
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23

Campos, Katherine Helen de Sa. "Identification and characterization of components that overcome secretion limitations of the yeast Pichia pastoris." Scholarly Commons, 2013. https://scholarlycommons.pacific.edu/uop_etds/844.

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Abstract (sommario):
The methylotrophic yeast. Pichia pastoris, is a powerful, adaptable, and inexpensive recombinant expression system commonly used to secrete heterologous protein. Although P. pastoris is a popular host organism, secretion inefficiency continues to be a major hurdle in its ability to produce high levels of foreign protein. Optimization of cis- and trans-acting factors has greatly enhanced the secretory capabilities of P. pastoris, however protein-specific engineering of a host organism is costly and not always effective. P. pastoris' secretion inefficiency is commonly due to trans-acting factors
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24

Maggi, Silviane. "Hemolinfa bruta e frações de Lonomia obliqua Walker, 1855 (Lepidoptera, Saturniidae : avaliação do perfil proteico e viabilidade celular em cultura hipocampal primária de ratos Wistar." reponame:Repositório Institucional da UCS, 2015. https://repositorio.ucs.br/handle/11338/1263.

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elevação da expectativa de vida e a diminuição no índice de natalidade têm como consequência o envelhecimento da população mundial, com aumento da incidência de doenças neurodegenerativas. Pessoas acometidas por esses males dispõem atualmente de limitados recursos terapêuticos. Estes muitas vezes são só paliativos, com baixa ou nula perspectiva quanto à reversão dos danos instalados. Por isso, o estudo de substâncias possivelmente neuroprotetoras, que apresentam potencial aplicação como novas drogas, tem sido alvo de pesquisas. Estudos realizados a partir das toxinas produzidas por lagartas da
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25

Corrocher, Flávia Adolfo. "Caracterização funcional de fatores de transcrição hipotéticos de Neurospora crassa /." Araraquara : [s.n.], 2012. http://hdl.handle.net/11449/88005.

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Orientador: Maria Célia Bertolini<br>Banca: Cleslei Fernando Zanelli<br>Banca: Iran Malavazi<br>Resumo: O fungo Neurospora crassa é um organismo amplamente utilizado como organismo modelo na compreensão de aspectos fundamentais da biologia dos eucariotos. Nosso laboratório tem utilizado este fungo para estudar os mecanismos bioquímicos e moleculares envolvidos na regulação do metabolismo do glicogênio. A avaliação de uma coleção de linhagens mutantes individualmente nocauteadas em genes que codificam fatores de transcrição permitiu identificar várias proteínas potencialmente envolvidas na regu
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26

Danial, John Shokri Hanna. "Imaging lipid phase separation on droplet interface bilayers." Thesis, University of Oxford, 2015. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.711943.

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27

Lee, Jooyoung. "Anti-CRISPR Proteins: Applications in Genome Engineering." eScholarship@UMMS, 2020. https://escholarship.umassmed.edu/gsbs_diss/1091.

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Clustered, regularly interspaced, short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) constitute a bacterial and archaeal adaptive immune system. The ongoing arms race between prokaryotic hosts and their invaders such as phages led to the emergence of anti-CRISPR proteins as countermeasures against the potent antiviral defense. Since the first examples of anti-CRISPRs were shown in a subset of CRISPR-Cas systems, we endeavored to uncover these naturally-occurring inhibitors that inactivate different types of CRISPR-Cas systems. In the first part of my thesis, we have identifi
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28

Pan, Tao. "Towards early stage disease detection in microdevices : fabrication and testing of micro total analysis systems for bioanalytical applications / /." Diss., CLICK HERE for online access, 2007. http://contentdm.lib.byu.edu/ETD/image/etd1836.pdf.

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29

Elgh, Fredrik. "Human antibody responses to hantavirus recombinant proteins & development of diagnostic methods." Doctoral thesis, Umeå universitet, Virologi, 1996. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-141300.

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Rodent-borne hantaviruses (family Bunyaviridae) cause two distinct human infections; hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). HFRS is a common viral zoonosis, characterized by fever, renal dysfunction and hemostatic imbalance. Four HFRS-associated hantaviruses have been described: Hantaan virus and Seoul virus mainly found in Asia, Dobrava virus, encountered in the Balkan region and Puumala virus (PUU), causing mild HFRS (nephropathia epidemica; NE) in Europe. HPS, recently discovered in the Americas, involves adult respiratory distress syndrome wit
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30

Nohoesu, Oviavo. "Characterization of SABP2-Interacting Proteins (SIP) 428: an NAD+-Dependent Deacetylase Enzyme in Plant Abiotic Stress Signaling." Digital Commons @ East Tennessee State University, 2021. https://dc.etsu.edu/etd/3961.

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Abiotic stress leads to a change in the water content of plants. Salinity and osmotic stress affect both the morphology and physiology of plants. Plants have therefore responded to these environmental changes by adapting and tolerating them. The SABP2-interacting proteins (SIP) 428-silenced RNAi transgenic tobacco lines were subjected to various abiotic stresses (salinity, osmotic, and drought). The effect of SIP428-silencing on the tobacco plants subjected to these abiotic stresses was monitored. The results from the root growth data show that the sip428-silenced lines exhibit enhanced tolera
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31

Greenham, Trevor. "Pointillism in Plant Systems Biology: I. Proteomic Analysis of Plant Exosome-like Particles II. Amyloplast-binding Puroindoline Fusion Proteins for Recombinant Protein Expression." Thesis, Université d'Ottawa / University of Ottawa, 2019. http://hdl.handle.net/10393/39647.

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Expanding upon our understanding of plant defense is critical, particularly with the perilous threats of climate change and overpopulation to our food security, health and well-being. In this study, we focused on plant defense using two distinct approaches. First, we performed a proteomic analysis of plant exosome-like nanoparticles in order to elucidate their defense related protein cargo. Secondly, we used a wheat antimicrobial protein, puroindoline, as a fusion partner for the expression of recombinant proteins in rice endosperm. Plant exosome-like nanoparticles (ELP) were isolated from fr
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32

Strannermyr, Malin. "Increased expression of proteins in CHO cells by identification of signal peptides for improved secretion of translated proteins." Thesis, Linköpings universitet, Teknisk biologi, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-150364.

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Main purpose of this study was to increase protein expression in Chinese hamster ovary (CHO) cells by improving protein secretion of translated proteins. The goal was to find signal peptides from the screening of signal peptide libraries for improvement of protein secretion using a CHO-cell express selection system. Biopharmaceutical products, proteins such as monoclonal antibodies (mAbs), are most commonly produced using mammalian expression systems such as the expression in CHO cells. The posttranslational modifications of the proteins being expressed in CHO cells are similar to the expressi
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33

Dennis, Allison Marie. "Quantum dot-fluorescent protein pairs as fluorescence resonance energy transfer pairs." Diss., Georgia Institute of Technology, 2009. http://hdl.handle.net/1853/37079.

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Fluorescence resonance energy transfer (FRET)-based biosensors have been designed to fluorometrically detect everything from proteolytic activity to receptor-ligand interactions and structural changes in proteins. While a wide variety of fluorophores have demonstrated effectiveness in FRET probes, several potential sensor components are particularly notable. Semiconductor quantum dots (QDs) are attractive FRET donors because they are rather bright, exhibit high quantum yields, and their nanoparticulate structure enables the attachment of multiple acceptor molecules. Fluorescent proteins (FPs)
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34

Lundell, Emma. "Interactions of the Human Recombinant Proteins JUNO and IZUMO1." Thesis, KTH, Skolan för kemi, bioteknologi och hälsa (CBH), 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-277123.

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Det uppskattas att 15% av alla par världen över lider avinfertilitet. Ungefär hälften beror på manlig infertilitet och 40% av dessa fall kan ännu inte förklaras. Därmed är nuvarande metoder för att diagnostisera manlig infertilitet otillräckliga och ytterligare tekniker behövs. En lyckad befruktning kräver att spermierna uttrycker membranproteinet Izumo1 som måste känna igen dess receptorprotein Juno, belägen vid ytan av äggmembranet. Bindningen mellan Juno och Izumo1 är essentiell för befruktning hos däggdjur då den bidrar till att gameternabinder och skapar en ny distinkt organism. Juno är e
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35

Brohée, Sylvain. "Etude bioinformatique du réseau d'interactions entre protéines de transport ches les Fungi." Doctoral thesis, Universite Libre de Bruxelles, 2008. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/210432.

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Les protéines associées aux membranes sont d'une importance cruciale pour la cellule. Cependant, en raison d'une plus grande difficulté de manipulation, les données biochimiques les concernant sont très lacunaires, notamment au point de vue de la formation de complexes entre ces protéines.<p><p>L'objectif global de notre travail consiste à combler ces lacunes et à préciser les interactions entre protéines membranaires chez la levure Saccharomyces cerevisiae et plus précisément, entre les transporteurs. Nous avons commencé notre travail par l'étude d'un jeu de données d'interactions à grande éc
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36

Venter, Alida. "The functional analysis of Vitaceae polygalacturonase-inhibiting protein (PGIP) encoding genes overexpressed in tobacco." Thesis, Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/4350.

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Thesis (MScAgric (Viticulture and Oenology. Wine Biotechnology))--University of Stellenbosch, 2010.<br>ENGLISH ABSTRACT: Agriculture worldwide is under great pressure to produce enough food in order to sustain the ever-growing world population. Among the many challenges faced by food producers, crop losses and damage caused by fungal plant pathogens is a major problem. The study of fungal pathogens and the interaction between plants and fungi is therefore essential, and has been carried out for many years. Much has been learned in this time, but the full mechanisms of the various modes of
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37

Einarsson, Ellen. "Evaluation of 5´- and 3´-UTR Translation Enhancing Sequences to Improve Translation of Proteins in CHO Cells." Thesis, Linköpings universitet, Teknisk biologi, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-150363.

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The purpose of this project was to identify and evaluate nucleotide sequences enhancing translation of proteins in Chinese hamster ovary (CHO) cells. Candidate sequences were placed in the 5´-untranslated region (UTR) or 3´ UTR respectively and evaluated in a CHO-based expression system with a fluorescent Fc-fusion protein as a model protein.Five plasmid vectors were constructed, two of which designed to have a randomized nucleotide library in their 5´ and 3´ UTR respectively, and three of which designed to hold varying repeats of a known enhancing translation (ET) sequence in their 5´ or 3´ U
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38

Campos, Katherine Helen de Sa. "Identification and characterization of components that overcome secretion limitations of the yeast Pichia pastoris : a thesis." Scholarly Commons, 2001. https://scholarlycommons.pacific.edu/uop_etds/844.

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39

Pal, Ramendra K. "Fabrication of flexible, biofunctional architectures from silk proteins." VCU Scholars Compass, 2017. http://scholarscompass.vcu.edu/etd/4995.

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Advances in the biomedical field require functional materials and processes that can lead to devices that are biocompatible, and biodegradable while maintaining high performance and mechanical conformability. In this context, a current shift in focus is towards natural polymers as not only the structural but also functional components of such devices. This poses material-specific functionalization and fabrication related questions in the design and fabrication of such systems. Silk protein biopolymers from the silkworm show tremendous promise in this regard due to intrinsic properties: mechani
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40

Gibert, Amat Jordi. "Producció de transglutaminasa de blat de moro (TGZ) en Escherichia coli i Pichia pastoris: Estudi i millora del procés." Doctoral thesis, Universitat Ramon Llull, 2017. http://hdl.handle.net/10803/416598.

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La transglutaminasa (TGasa) és un enzim que catalitza modificacions postraduccionals de proteïnes mitjançant enllaços ε-(γ-glutamil) i ponts covalents d’amida. En plantes, aquest enzim està poc estudiat, i només ha estat clonat el gen TGasa de Zea mays (tgz). Aquesta tesi resumeix la feina feta per desenvolupar un sistema d'expressió utilitzant dos microorganismes recombinants, Escherichia coli (E. coli) i Pichia pastoris (P. pastoris) per produir i caracteritzar l'enzim transglutaminasa (TGZ). Treballs anteriors en E. coli van mostrar que la proteïna recombinant era present principalment en
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41

Silva, Elisangela Teixeira da. "Estabilização de proteases para aplicação tecnológica." Universidade Católica de Pernambuco, 2013. http://www.unicap.br/tede//tde_busca/arquivo.php?codArquivo=889.

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Enzimas são biocatalisadores específicos que são utilizadas em vários campos de atuação, desde a indústria de alimentos, até na formulação de detergentes. As proteases são biocatalisadores de grande interesse comercial na indústria, movimentando bilhões de dólares com produção de toneladas de detergentes para diferentes aplicações. A demana de proteases no mercado brasileiro promove pesquisas como também o empreendorismo nesse segmento embora mais investimentos por parte das agências do governo devem ser necessárias. A potencialidade da matéria prima renovável e o aumento do desenvolviemnto
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42

Silva, Fernanda Cristina Padilha da Rocha e. "Estudo teórico-experimental da separação gravitacional de emulsões compostas por água do mar, derivados de petróleo e biossurfactantes." Universidade Católica de Pernambuco, 2014. http://www.unicap.br/tede//tde_busca/arquivo.php?codArquivo=971.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico<br>As refinarias de petróleo, assim como outros processos industriais em grande escala, são fontes potenciais de poluição ambiental. Os acidentes ocorridos com derramamento de petróleo e seus derivados no Brasil, no período de 1975 a 2012, somam milhões de litros de poluentes que promoveram a contaminação de solos, rios e mar. Os processos fisíco-químicos tais como, a centrifugação, ultrafiltração e flotação por ar dissolvido (FAD), podem ser eficazes quando usados para separar óleos emulsionados. Nesse sentido, o processo de FAD co
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43

Gnesa, Eric Henry. "The conserved C-terminal domain of spider tubuliform spidroin 1 contributes to extensibility in synthetic fibers." Scholarly Commons, 2011. https://scholarlycommons.pacific.edu/uop_etds/771.

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Spider silk is renowned for its extraordinary mechanical properties, having a balance of high tensile strength and extensibility. To date, the majority of studies have focused on the production of dragline silks from synthetic spider silk gene products. Here we report the first mechanical analysis of synthetic egg case silk fibers spun from the Latrodectus hesperus tubuliform silk proteins, TuSp1 and ECP-2. We provide evidence that recombinant ECP-2 proteins can be spun into fibers that display mechanical properties similar to other synthetic spider silks. We also demonstrate that silks spun f
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44

Reis, Marina von Atzingen dos. "Expressão, purificação e caracterização de proteínas de superfície de Leptospira interrogans." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-13082009-093341/.

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O sequenciamento genômico da L. interrogans sorovar Copenhageni e ferramentas de bioinformática nos permitiram selecionar 15 genes que codificam proteínas hipotéticas conservadas preditas de superfície. A conservação foi confirmada por PCR em seis dos sorovares mais predominantes de L. interrogans. As proteínas recombinantes foram clonadas em vetor de expressão de E. coli em fusão com seis resíduos de histidina, que permite a purificação por cromatografia de afinidade a metal. Seis proteínas apresentaram reatividade com anticorpos presentes em soros de pacientes com leptospirose. Através de um
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45

Zanol, Franciele Maria. "Caracterização molecular e funcional de PA0657, uma protease ATP-dependente de Pseudomonas aeruginosa." reponame:Repositório Institucional da UCS, 2013. https://repositorio.ucs.br/handle/11338/886.

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Uma característica associada à capacidade de Pseudomonas aeruginosa sobreviver e disseminar-se frente a situações adversas encontradas no ambiente e no organismo de hospedeiros é a regulação de genes de resposta a condições de estresse celular, que incluem o evento de choque térmico. A exposição do microrganismo a um ambiente de alta temperatura resulta na indução da síntese de proteínas específicas, representadas por chaperonas e proteases, que conferem um aumento da viabilidade celular microbiana em condições consideradas letais. Presume- se que o gene PA 0657 de P. aeruginosa O1 possa estar
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46

Volk, Anna-Luisa. "Cell line and protein engineering tools for production and characterization of biologics." Doctoral thesis, KTH, Proteomik och nanobioteknologi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-212931.

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Our increasing understanding of disease mechanisms coupled with technological advances has facilitated the generation of pharmaceutical proteins, which are able to address yet unmet medical needs. Diseases that were fatal in the past can now be treated with novel biological medications improving and prolonging life for many patients. Pharmaceutical protein production is, however, a complex undertaking, which is by no means problem-free. The demand for more complex proteins and the realization of the importance of post-translational modifications have led to an increasing use of mammalian cells
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47

Kubo, Tatiana Miyuki Ogawa. "Preparação e caracterização de microparticulas de hialuronato de sodio para encapsulação e liberação controlada de proteinas para aplicação nasal." [s.n.], 2005. http://repositorio.unicamp.br/jspui/handle/REPOSIP/267236.

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Orientador: Maria Helena Andrade Santana<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia Quimica<br>Made available in DSpace on 2018-08-04T23:14:50Z (GMT). No. of bitstreams: 1 Kubo_TatianaMiyukiOgawa_M.pdf: 3390196 bytes, checksum: 90fbf573dfcb12a87da26f2fdcbfb4dd (MD5) Previous issue date: 2005<br>Resumo: Neste trabalho foi feito o estudo da preparação de microesferas de hialuronato de sódio pelo método de emulsificação e evaporação de solvente, utilizando temperatura como agente de retificação física. O assunto foi abordado com ênfase na influência d
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48

Viana, Carolina de AraÃjo. "CaracterizaÃÃo bioquÃmica e atividades biolÃgicas de quitinases laticÃferas de Calotropis procera." Universidade Federal do CearÃ, 2015. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=15513.

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CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior<br>Calotropis procera à uma planta laticÃfera, pertencente à famÃlia Apocynaceae. ProteÃnas de defesa foram descritas no lÃtex com atividade contra insetos fitÃfagos e fungos fitopatogÃnicos. A fraÃÃo proteica do lÃtex tambÃm apresenta atividades farmacolÃgicas, dentre tais efeito citotÃxico seletivo em cÃlulas carcinogÃnicas. A partir da fraÃÃo proteica efetiva sobre cÃlulas neoplÃsicas, o trabalho teve por meta central identificar proteÃna(s) citotÃxica(s) e proceder a sua caracterizaÃÃo bioquÃmica, biolÃgica e suas perspectivas bio
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49

Dehouck, Yves. "Développement de potentiels statistiques pour l'étude in silico de protéines et analyse de structurations alternatives." Doctoral thesis, Universite Libre de Bruxelles, 2005. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/211040.

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Cette thèse se place dans le cadre de l'étude in silico, c'est-à-dire assistée par ordinateur, des liens qui unissent la séquence d'une protéine à la (ou aux) structure(s) tri-dimensionnelle(s) qu'elle adopte. Le décryptage de ces liens présente de nombreuses applications dans divers domaines et constitue sans doute l'une des problématiques les plus fascinantes de la recherche en biologie moléculaire.<p><p>Le premier aspect de notre travail concerne le développement de potentiels statistiques dérivés de bases de données de protéines dont les structures sont connues. Ces potentiels présentent p
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50

Becker, John van Wyk 1975. "Evaluation of the role of PGIPs in plant defense responses." Thesis, Stellenbosch : Stellenbosch University, 2007. http://hdl.handle.net/10019.1/17476.

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Dissertation (PhD)--University of Stellenbosch, 2007.<br>ENGLISH ABSTRACT: Plants have developed sophisticated means of combating plant diseases. The events that prepare the plant for, and follow plant-pathogenic interactions, are extremely complex and have been the topic of intensive investigation in recent years. These interactions involve a plethora of genes and proteins, and intricate regulation thereof; from the host and pathogen alike. Studying the contribution of single genes and their encoded proteins to the molecular dialogue between plant and pathogen has been a focus of plant
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