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Artykuły w czasopismach na temat „16S rDNA sequence of S”

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1

Tajbakhsh, Mercedeh, Babak Noory Nayer, Kamyar Motavaze, et al. "Phylogenetic relationship of Salmonella enterica strains in Tehran, Iran, using 16S rRNA and gyrB gene sequences." Journal of Infection in Developing Countries 5, no. 06 (2011): 465–72. http://dx.doi.org/10.3855/jidc.1504.

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Introduction: We assessed whether 16S rDNA and gyrB gene sequences, alone or combined, were suitable for determining the phylogenetic relationship among Salmonella enterica strains isolated from Tehran, Iran. Patients over five years of age enrolled in an acute diarrheal surveillance project in Tehran province between May 2004 and October 2006 were selected as our study group. Methodology: 16S ribosomal DNA (rDNA) and gyrB genes from 40 Salmonella isolates obtained from patients with acute diarrhea were sequenced and the data was used to generate phylogenetic trees that facilitated isolate com
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Rome, Sophie, Jean-Claude Cleyet-Marel, Luis A. Materon, Philippe Normand, and Brigitte Brunel. "Rapid identification of Medicago nodulating strains by using two oligonucleotide probes complementary to 16S rDNA sequences." Canadian Journal of Microbiology 43, no. 9 (1997): 854–61. http://dx.doi.org/10.1139/m97-124.

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Symbiotic bacteria associated with the Medicago genus are separated into two closely related species named Sinorhizobium meliloti and Sinorhizobium medicae. To discriminate rapidly between these two bacterial species, two 15-base DNA probes, 16Smfs and 16Smed, were designed from the alignment of 16S rDNA sequences to differentiate S. meliloti from S. medicae. Their specificities were evaluated by dot-blot hybridization experiments on 25 reference strains representing 13 species of Rhizobium and Sinorhizobium, and by comparison with all 16S rDNA sequences available in the GenBank data base. No
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3

Valiunas, D., R. Jomantiene, A. Ivanauskas, et al. "First Report of a New Phytoplasma Subgroup, 16SrIII-T, Associated with Decline Disease Affecting Sweet and Sour Cherry Trees in Lithuania." Plant Disease 93, no. 5 (2009): 550. http://dx.doi.org/10.1094/pdis-93-5-0550b.

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During July 2007, sweet (Prunus avium) and sour cherry (P. cerasus) trees exhibiting disease symptoms suggestive of possible phytoplasma infection were observed in a large orchard in the Kaunas Region of Lithuania. Samples of leaf tissue were collected from 13 sweet cherry trees that were affected by a decline disease (designated cherry decline, ChD) characterized by symptoms that included leaf reddening and premature leaf drop and two sour cherry trees exhibiting proliferation of branches and nonseasonal flowering. To assess the diseased trees for phytoplasma infection, DNA was extracted with
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Nogales, Balbina, Edward R. B. Moore, Enrique Llobet-Brossa, Ramon Rossello-Mora, Rudolf Amann, and Kenneth N. Timmis. "Combined Use of 16S Ribosomal DNA and 16S rRNA To Study the Bacterial Community of Polychlorinated Biphenyl-Polluted Soil." Applied and Environmental Microbiology 67, no. 4 (2001): 1874–84. http://dx.doi.org/10.1128/aem.67.4.1874-1884.2001.

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ABSTRACT The bacterial diversity assessed from clone libraries prepared from rRNA (two libraries) and ribosomal DNA (rDNA) (one library) from polychlorinated biphenyl (PCB)-polluted soil has been analyzed. A good correspondence of the community composition found in the two types of library was observed. Nearly 29% of the cloned sequences in the rDNA library were identical to sequences in the rRNA libraries. More than 60% of the total cloned sequence types analyzed were grouped in phylogenetic groups (a clone group with sequence similarity higher than 97% [98% for Burkholderia andPseudomonas-ty
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5

Deng, Cheng Xun, Mei Yue, Ling Wang, and Yu Hui Li. "Molecular Identification of a Strain Acidithiobacillus Ferrooxidans and its Biological Characteristics." Advanced Materials Research 402 (November 2011): 3–6. http://dx.doi.org/10.4028/www.scientific.net/amr.402.3.

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A strain of Acidithiobacillus ferrooxidans designated S t1 was isolated and identified from the acid mine drainage of Tianmashan Coal Mine, Tongling county, Anhui province. Its morphological, physiological and biochemical characters, growth conditions of pH value and temperature, 16S rDNA sequence and the phylogenetic tree was studied. Results showed that the bacteria strains from different sites have divergence at the curve of pH and Eh values during the enriching culture. The isolated strain S t1 grew in a pH rang from 2.0 to 3.0 and temperature 25 to 35°C with over 98％similarity toAcidithio
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Texeira, D. C., J. Ayres, E. W. Kitajima, et al. "First Report of a Huanglongbing-Like Disease of Citrus in Sao Paulo State, Brazil and Association of a New Liberibacter Species, “Candidatus Liberibacter americanus”, with the Disease." Plant Disease 89, no. 1 (2005): 107. http://dx.doi.org/10.1094/pd-89-0107a.

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Huanglongbing (HLB) (ex-greening) is one of the most serious diseases of citrus. The causal agent is a noncultured, sieve tube-restricted α-proteobacterium, “Candidatus Liberibacter africanus” in Africa and “Candidatus Liberibacter asiaticus” in Asia (2). The disease has never been reported from the American continent. However, Diaphorina citri, the Asian psyllid vector of HLB, is found in South, Central, and North America (Florida and Texas). Early in 2004, leaf and fruit symptoms resembling those of HLB were observed in several sweet orange orchards near the city of Araraquara, Sao Paulo Sta
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Layton, A. C., P. N. Karanth, C. A. Lajoie, et al. "Quantification of Hyphomicrobium Populations in Activated Sludge from an Industrial Wastewater Treatment System as Determined by 16S rRNA Analysis." Applied and Environmental Microbiology 66, no. 3 (2000): 1167–74. http://dx.doi.org/10.1128/aem.66.3.1167-1174.2000.

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ABSTRACT The bacterial community structure of the activated sludge from a 25 million-gal-per-day industrial wastewater treatment plant was investigated using rRNA analysis. 16S ribosomal DNA (rDNA) libraries were created from three sludge samples taken on different dates. Partial rRNA gene sequences were obtained for 46 rDNA clones, and nearly complete 16S rRNA sequences were obtained for 18 clones. Seventeen of these clones were members of the beta subdivision, and their sequences showed high homology to sequences of known bacterial species as well as published 16S rDNA sequences from other a
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Gomes, Eliane Aparecida, Sílvia Neto Jardim, Claudia Teixeira Guimarães, Isabel Regina Prazeres de Souza, and Elizabeth de Oliveira. "Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plants." Pesquisa Agropecuária Brasileira 39, no. 1 (2004): 61–65. http://dx.doi.org/10.1590/s0100-204x2004000100009.

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The objective of this work was to characterize the genetic variability of phytoplasma and Spiroplasma kunkelii isolated from maize plants showing symptoms of stunt collected from different Brazilian geographic regions. A DNA fragment of 500 base pairs (bp) was amplified from the spiralin gene in S. kunkelii and one fragment of 1,200 bp was generated from 16S rDNA gene in phytoplasma. The partial sequences of the spiralin gene showed similarity of 98% among the isolates of S. kunkelii analyzed. These sequences were compared with the sequence of the spiralin gene from other Spiroplasma species d
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Ma, Xiping, Xianzhu Li, Wanlong Li, Di Wang, Chengbin Xu, and Xuelian Meng. "Identification and characterization of a cold-adapted and halotolerant nitrobenzene-degrading bacterium." RSC Advances 5, no. 98 (2015): 80276–82. http://dx.doi.org/10.1039/c5ra14280f.

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Coombs, Justin T., and Christopher M. M. Franco. "Isolation and Identification of Actinobacteria from Surface-Sterilized Wheat Roots." Applied and Environmental Microbiology 69, no. 9 (2003): 5603–8. http://dx.doi.org/10.1128/aem.69.9.5603-5608.2003.

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ABSTRACT This is the first report of filamentous actinobacteria isolated from surface-sterilized root tissues of healthy wheat plants (Triticum aestivum L.). Wheat roots from a range of sites across South Australia were used as the source material for the isolation of the endophytic actinobacteria. Roots were surface-sterilized by using ethanol and sodium hypochlorite prior to the isolation of the actinobacteria. Forty-nine of these isolates were identified by using 16S ribosomal DNA (rDNA) sequencing and found to belong to a small group of actinobacterial genera including Streptomyces, Microb
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Rascoe, J., M. Berg, U. Melcher, et al. "Identification, Phylogenetic Analysis, and Biological Characterization of Serratia marcescens Strains Causing Cucurbit Yellow Vine Disease." Phytopathology® 93, no. 10 (2003): 1233–39. http://dx.doi.org/10.1094/phyto.2003.93.10.1233.

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A serious vine decline of cucurbits known as cucurbit yellow vine disease (CYVD) is caused by rod-shaped bacteria that colonize the phloem elements. Sequence analysis of a CYVD-specific polymerase chain reaction (PCR)-amplified 16S rDNA product showed the microbe to be a γ-proteobacterium related to the genus Serratia. To identify and characterize the bacteria, one strain each from watermelon and zucchini and several noncucurbit-derived reference strains were subjected to sequence analysis and biological function assays. Taxonomic and phylogenetic placement was investigated by analysis of the
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Komaki, Hisayuki, and Tomohiko Tamura. "Differences at Species Level and in Repertoires of Secondary Metabolite Biosynthetic Gene Clusters among Streptomyces coelicolor A3(2) and Type Strains of S. coelicolor and Its Taxonomic Neighbors." Applied Microbiology 1, no. 3 (2021): 573–85. http://dx.doi.org/10.3390/applmicrobiol1030037.

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Streptomyces coelicolor A3(2) is used worldwide for genetic studies, and its complete genome sequence was published in 2002. However, as the whole genome of the type strain of S. coelicolor has not been analyzed, the relationship between S. coelicolor A3(2) and the type strain is not yet well known. To clarify differences in their biosynthetic potential, as well as their taxonomic positions, we sequenced whole genomes of S. coelicolor NBRC 12854T and type strains of its closely related species—such as Streptomyces daghestanicus, Streptomyces hydrogenans, and Streptomyces violascens—via PacBio.
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Munyaneza, J. E., V. G. Sengoda, J. M. Crosslin, J. A. Garzón-Tiznado, and O. G. Cardenas-Valenzuela. "First Report of “Candidatus Liberibacter solanacearum” in Tomato Plants in México." Plant Disease 93, no. 10 (2009): 1076. http://dx.doi.org/10.1094/pdis-93-10-1076a.

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Tomato (Solanum lycopersicum) plants exhibiting symptoms resembling those of permanent yellowing disease (known in Mexico as “permanente del tomate”) that is commonly associated with phytoplasmas (1) were observed in tomato fields in Sinaloa, México in March 2009. Plant symptoms also resembled those caused by “Candidatus Liberibacter solanacearum” infection (2). Affected plants showed an overall chlorosis, severe stunting, leaf cupping, purple discoloration of veins, excessive branching of axillary shoots, and leaf scorching (1,2). Symptom incidence ranged from 18 to 40%. To investigate whethe
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Pellegrin, Véronique, Stefan Juretschko, Michael Wagner, and Gilles Cottenceau. "Morphological and Biochemical Properties of aSphaerotilus sp. Isolated From Paper Mill Slimes." Applied and Environmental Microbiology 65, no. 1 (1999): 156–62. http://dx.doi.org/10.1128/aem.65.1.156-162.1999.

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ABSTRACT Four strains of filamentous bacteria were isolated from slimes collected in different paper mill factories. Morphological and physiological characterization of the isolates indicated an affiliation with the genus Sphaerotilus. However, while the physiological properties of the isolates were almost identical, pronounced physiological differences between the isolates andSphaerotilus natans DSM 6575T, DSM 565, and DSM 566 with respect to their ability to metabolize complex polysaccharides, sugars, polyalcohols, or organic acids as carbon sources were detected. In contrast to the analyzed
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15

Li, Z. N., H. Min, Y. Yan, Z. Zhao, W. J. Wu, and Y. F. Wu. "First Report of Syringa oblata and S. reticulata Leafroll Disease in China." Plant Disease 93, no. 3 (2009): 322. http://dx.doi.org/10.1094/pdis-93-3-0322c.

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Syringa oblata is an important ornamental tree widely grown in China. In September of 2008, S. oblata plants exhibiting symptoms of leafroll and yellowing were found in a garden on the Northwest A&F University campus. Samples were collected from this site. Total DNA was extracted from 0.5 g of phloem tissue from leaf midribs and stems of each sample. DNA samples were analyzed with a nested PCR assay using phytoplasma 16S rDNA universal primers R16mF2/R16mR1 followed by specific primers R16F2n/R16R2 (1), which amplified a 1,452- and 1,246-bp product, respectively. We tested all 30 lilac sam
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Stunžėnas, Virmantas, Romualda Petkevičiūtė, and Gražina Stanevičiūtė. "Phylogeny of Sphaerium solidum (Bivalvia) based on karyotype and sequences of 16S and ITS1 rDNA." Open Life Sciences 6, no. 1 (2011): 105–17. http://dx.doi.org/10.2478/s11535-010-0101-6.

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AbstractThe present work represents the first karyological and molecular characterisation of Sphaerium solidum, a rare European clam. Specimens of S. solidum were collected in Lithuania and Hungary. The modal diploid chromosome number found in both populations was 2n=30. Small, biarmed B chromosomes were found in 42.3% of cells studied in clams from Lithuania and in 11.8% of cells in clams from Hungary. Comparative analysis revealed no significant (P<0.05) interspecific differences in chromosome morphology of S. solidum and that of previously studied S. corneum. DNA sequence analyses of S.
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Urbanaviciene, L., R. Jomantiene, and R. E. Davis. "First Report of Barley as Host of a Phytoplasma Belonging to Group 16SrI, Subgroup B, and Ribosomal Protein Subgroup rpI-B in Lithuania." Plant Disease 89, no. 3 (2005): 339. http://dx.doi.org/10.1094/pd-89-0339a.

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Numerous diseased plants of barley (Hordeum vulgaris L.) exhibiting twisted, abnormally thin and yellowed awns, reduced spikelets, and general stunting and yellowing were observed in fields in the Vilnius and Kaisiadorys regions of Lithuania. The possible association of a phyto-plasma with the disease, termed barley deformation (BaDef), was assessed using polymerase chain reaction (PCR). Three phytoplasma universal primer pairs (P1/P7, R16F2n/R16R2, and rpF1/rpR1) (1,2,4) were employed to amplify ribosomal (r) RNA gene (rDNA) and ribosomal protein (rp) gene sequences. Template DNA extractions
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TAWONG, WITTAYA, PONGSANAT PONGCHAROEN, TOMOHIRO NISHIMURA, and WEERACHAI SAIJUNTHA. "Siamcapillus rubidus gen. et sp. nov. (Oculatellaceae), a novel filamentous cyanobacterium from Thailand based on molecular and morphological analyses." Phytotaxa 558, no. 1 (2022): 33–52. http://dx.doi.org/10.11646/phytotaxa.558.1.2.

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Cyanobacterial diversity has been extensively studied; however, scant knowledge exists concerning genetic data and higher accuracy for tropical cyanobacterial diversity. In particular, the existence of unnamed monophyletic genera within the Oculatellaceae requires further investigation. Here, two novel strains (NUACC13 and NUACC14) were isolated from a cyanobacterial mat covering border soil of a small freshwater pond in Phitsanulok Province, Thailand. A polyphasic approach combining genetic (16S rDNA and rpoC1 gene phylogenies, evolutionary distance and ITS secondary structures) and morpholog
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Zhang, Chun Hong, Dong Dong Xiao, Pei Shi Sun, Ping Zou, and Qiu Ling Chen. "Screening, Identification and Characterization of a Cyanoethylated Wastepaper-Degrading Bacteria." Applied Mechanics and Materials 316-317 (April 2013): 349–53. http://dx.doi.org/10.4028/www.scientific.net/amm.316-317.349.

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A cyanoethylated wastepaper-degrading bacterial strain S11 was screened from the surface humus soil in the forest of Yunnan University. The bacterium was identified through analysis of morphological, biological and biochemical features and 16S rRNA sequence. S11 enjoyed above 99% homology with Ochrobactrum sp.CRRI29’s 16S rDNA sequence, therefore the baterium belonged to Ochrobactrum sp. The research on degradation features of S11 revealed that S11 bacterium could effectively degrade cyanoethylated wastepaper under 35°C, pH 11.0, 25% inoculum size and 50g/L substrate concentration; under the o
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Munyaneza, J. E., V. G. Sengoda, J. M. Crosslin, J. A. Garzón-Tiznado, and O. G. Cardenas-Valenzuela. "First Report of “Candidatus Liberibacter solanacearum” in Pepper Plants in México." Plant Disease 93, no. 10 (2009): 1076. http://dx.doi.org/10.1094/pdis-93-10-1076b.

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Bell pepper (Capsicum annuum) plants exhibiting symptoms that resembled those of potato psyllid (Bactericera cockerelli Sulc) damage and “Candidatus Liberibacter solanacearum” infection (2) were observed in a pepper field in La Cruz de Elota, Sinaloa, México in March 2009, with an infection rate of 1.5%. Plants exhibited chlorotic or pale green apical growth and leaf cupping, sharp tapering of the leaf apex, shortened internodes, and an overall stunting (2). Total DNA was extracted from the top whole leaf tissue of nine symptomatic and five asymptomatic pepper plants with cetyltrimethylammoniu
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Acinas, Silvia G., Josefa Antón, and Francisco Rodríguez-Valera. "Diversity of Free-Living and Attached Bacteria in Offshore Western Mediterranean Waters as Depicted by Analysis of Genes Encoding 16S rRNA." Applied and Environmental Microbiology 65, no. 2 (1999): 514–22. http://dx.doi.org/10.1128/aem.65.2.514-522.1999.

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ABSTRACT In a previous study (S. G. Acinas, F. Rodrı́guez-Valera, and C. Pedrós-Alió, FEMS Microbiol. Ecol. 24:27–40, 1997), community fingerprinting by 16S rDNA restriction analysis applied to Mediterranean offshore waters showed that the free-living pelagic bacterial community was very different from the bacterial cells aggregated or attached to particles of more than about 8 μm. Here we have studied both assemblages at three depths (5, 50, and 400 m) by cloning and sequencing the 16S rDNA obtained from the same samples, and we have also studied the samples by scanning electron microscopy
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Sun, Yu Ying, Shu Jun Wang, and Ji Quan Zhang. "Cloning and Recombinant Expression of Chitosanase Gene from Bacillus Sp. S-1." Advanced Materials Research 343-344 (September 2011): 994–99. http://dx.doi.org/10.4028/www.scientific.net/amr.343-344.994.

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In this work, we report the characterization of a chitosanase-producing bacterium isolated from soil. This strain was grouped under the genus Bacillus by virtue of its morphological, physiological properties and 16S rDNA gene sequence and named it Bacillus sp. S-1. According to the information of chitosanase full-length sequences deposited in NCBI, a pair of degenerated primes was designed and a partial sequence of chitosanase gene was obtained by polymerase chain reaction (PCR) using Bacillus sp. S-1 genome DNA as the template. A genome walking library was constructed followed as the protocol
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Guo, F. L., H. Y. Zhang, X. M. Yu, W. Q. Zhao, D. Q. Liu, and C. Goyer. "First Report of Streptomyces galilaeus Associated with Common Scab in China." Plant Disease 98, no. 5 (2014): 683. http://dx.doi.org/10.1094/pdis-07-13-0699-pdn.

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During a survey of potato scab pathogens in China from 2003 to 2012, a new pathogen was found in Shanxi and Neimenggu provinces. The incidence was approximately 20% of all recovered strains. The lesions caused by the pathogen were slightly raised and similar to those caused by Streptomyces scabies (3). Lesions were excised (approximately 10 mm3) from 40 infected tubers, surface-disinfested with 0.3% NaOCl for 30 s, rinsed in sterile water three times, cut into 5 mm3, then sliced into 1-mm pieces, and plated on water agar amended with ampicillin (50 μg/ml). Plates were incubated at 28°C in the
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Siddiqui, Huma, Karin Lagesen, Alexander J. Nederbragt, Lars M. Eri, Stig L. Jeansson, and Kjetill S. Jakobsen. "Pathogens in Urine from a Female Patient with Overactive Bladder Syndrome Detected by Culture-independent High Throughput Sequencing: A Case Report." Open Microbiology Journal 8, no. 1 (2014): 148–53. http://dx.doi.org/10.2174/1874285801408010148.

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Introduction:Overactive bladder syndrome (OAB) is described as urgency, with or without urgency incontinence. A range of medical conditions shares the symptoms of OAB, however the diagnosis is contingent on the exclusion of urinary tract infection (UTI). Knowing that urine dipstick and routine culture of bacteria can miss UTI diagnosis caused by low-count bacteriuria or “difficult-to-culture” pathogens, we examined a case of OAB with a culture-independent approach.Case presentation:A 61-year-old Norwegian female with a long history of urinary symptoms and a diagnosis of OAB was selected as a s
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Rachman, Cinta, Petia Kabadjova, Rosica Valcheva, Hervé Prévost, and Xavier Dousset. "Identification of Carnobacterium Species by Restriction Fragment Length Polymorphism of the 16S-23S rRNA Gene Intergenic Spacer Region and Species-Specific PCR." Applied and Environmental Microbiology 70, no. 8 (2004): 4468–77. http://dx.doi.org/10.1128/aem.70.8.4468-4477.2004.

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ABSTRACT The genus Carnobacterium is currently divided into the following eight species: Carnobacterium piscicola, C. divergens, C. gallinarum, C. mobile, C. funditum, C. alterfunditum, C. inhibens, and C. viridans. An identification tool for the rapid differentiation of these eight Carnobacterium species was developed, based on the 16S-23S ribosomal DNA (rDNA) intergenic spacer region (ISR). PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of this 16S-23S rDNA ISR was performed in order to obtain restriction profiles for all of the species. Three PCR amplicons, which were desi
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Gumtow, R. L., A. A. Khan, A. M. Bocsanczy, J. M. F. Yuen, A. J. Palmateer, and D. J. Norman. "First Report of a Leaf Spot Disease of Golden Dewdrop (Duranta erecta) Caused by Pseudomonas cichorii and a Xanthomonas Species in Florida." Plant Disease 97, no. 6 (2013): 836. http://dx.doi.org/10.1094/pdis-12-12-1117-pdn.

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Duranta erecta (Verbenaceae) is used extensively in southern states as an ornamental shrub and has replaced boxwood as the most common short hedge accenting flower beds. Over the past 2 years, during warm wet periods, dark necrotic leaf spots have been observed on golden dewdrop plants in Florida. Isolations from these spots on Difco nutrient agar (NA) consistently yielded two types of bacterial colonies that were not always simultaneously present: 1) round butyrous, bright yellow and 2) flat cream-colored. Both were 2 mm in size after 48 h, gram-negative, and produced a hypersensitivity react
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Wu, Yeying, Yalan Feng, Mingyang Xue, et al. "Isolation and Identification of Staphylococcus saprophyticus from Diseased Hybrid Sturgeon." Fishes 8, no. 5 (2023): 250. http://dx.doi.org/10.3390/fishes8050250.

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Hybrid sturgeon is an important economic fish species in China. In 2021, a bacterium was isolated from the liver and kidneys of freshwater-farmed hybrid sturgeon in Yichang City, Hubei Province, causing a disease with high mortality and surface bleeding. Through morphological observation, 16S rDNA gene sequence analysis, pathogenicity, an antimicrobial sensitivity test, as well as serum physiological and biochemical analysis, it was identified as Staphylococcus saprophyticus and named E702. The 16S rDNA gene sequence of E702 is highly homologous to S. saprophyticus in GenBank. Phylogenetic ana
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Crosslin, J. M., P. B. Hamm, J. E. Eggers, S. I. Rondon, V. G. Sengoda, and J. E. Munyaneza. "First Report of Zebra Chip Disease and “Candidatus Liberibacter solanacearum” on Potatoes in Oregon and Washington State." Plant Disease 96, no. 3 (2012): 452. http://dx.doi.org/10.1094/pdis-10-11-0894.

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In August of 2011, potato (Solanum tuberosum) tubers grown in the lower Columbia Basin of southern Washington State and northern Oregon were observed with internal discolorations suggestive of zebra chip disease (ZC). Symptoms included brown spots, streaks, and stripes in and near the vascular tissue, typical of ZC (1). Symptoms were observed in cvs. Alturas, Russet Norkotah, Pike, Ranger Russet, Umatilla Russet, and Russet Burbank. Foliar symptoms on plants that produced symptomatic tubers included purple discoloration in upper leaves, leaf rolling, axial bud elongation, chlorosis, leaf scorc
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Foissac, Xavier, Jean-Luc Danet, Leyla Zreik, et al. "Cloning of the spoT Gene of “CandidatusPhlomobacter fragariae” and Development of a PCR-Restriction Fragment Length Polymorphism Assay for Detection of the Bacterium in Insects." Applied and Environmental Microbiology 66, no. 8 (2000): 3474–80. http://dx.doi.org/10.1128/aem.66.8.3474-3480.2000.

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ABSTRACT Marginal chlorosis is a new disease of strawberry in which the uncultured phloem-restricted proteobacterium “CandidatusPhlomobacter fragariae” is involved. In order to identify the insect(s) vector(s) of this bacterium, homopteran insects have been captured. Because a PCR test based on the 16S rRNA gene (rDNA) applied to these insects was unable to discriminate between “P. fragariae” and other insect-associated proteobacteria, isolation of “P. fragariae” genes other than 16S rDNA was undertaken. Using comparative randomly amplified polymorphic DNAs, an amplicon was specifically amplif
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30

Lee, Ing-Ming, Kristi D. Bottner, Joseph E. Munyaneza, et al. "Carrot Purple Leaf: A New Spiroplasmal Disease Associated with Carrots in Washington State." Plant Disease 90, no. 8 (2006): 989–93. http://dx.doi.org/10.1094/pd-90-0989.

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During the growing seasons of 2003 and 2004, a disease occurred in several carrot crops in south central Washington with symptoms suggestive of infection by phytopathogenic mollicutes (phytoplasmas and spiroplasmas). In the fall, many affected carrot plants exhibited extensive purple or yellow-purple leaf discoloration, general stunting of shoots and taproots, and formation of bunchy, fibrous secondary roots. For detection of the putative causal agents, polymerase chain reaction (PCR) assays were performed using primers specific to phytoplasmas as well as primers specific to plant-pathogenic s
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Saeed, S. T., A. Khan, and A. Samad. "First Report on the Molecular Identification of Phytoplasma (16SrII-D) Associated with Witches' Broom of Kalmegh (Andrographis paniculata) in India." Plant Disease 99, no. 1 (2015): 155. http://dx.doi.org/10.1094/pdis-08-14-0854-pdn.

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Andrographis paniculata (family Acanthaceae), also known as “King of Bitters” or Kalmegh, is an important medicinal plant used for the treatment of various diseases. It has antimicrobial, antiviral, anti-inflammatory, hepatoprotective, antidiabetic, antihyperglycemic, and antioxidant properties (1). During June 2014, while performing a routine survey of the commercial trial fields of Kalmegh at Central Institute of Medicinal and Aromatic Plants (CIMAP), Lucknow, India, typical phytoplasma disease symptoms such as virescence, proliferation, and witches' broom along with little leaf and stunted
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Ren, Y. Z., Y. Q. Liu, S. L. Ding, G. Y. Li, and H. Zhang. "First Report of Boll Rot of Cotton Caused by Pantoea agglomerans in China." Plant Disease 92, no. 9 (2008): 1364. http://dx.doi.org/10.1094/pdis-92-9-1364b.

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Since the summer of 2006, bacterial boll rot of cotton has been observed on fruits of ‘Xinluzao 31’ (Xinluzao 6 × Acala) in Xinjiang Province. It resulted in as much as 20% yield loss in several fields. Symptoms do not appear on the outer carpel. In the infected cotton bolls, fibers do not mature completely and seed tissue exhibits brown necrotic coloration. Lint and seeds from 24 surface-disinfested cotton bolls were triturated and plated onto King's medium B (KB). Plates were incubated at 28°C for 48 h. Forty eight strains with yellow pigmentation on KB were characterized. All were nonfluore
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Stefan, Andrei, Jannick Van Cauwenberghe, Craita Maria Rosu, et al. "Nodules of Medicago spp. Host a Diverse Community of Rhizobial Species in Natural Ecosystems." Agronomy 14, no. 9 (2024): 2156. http://dx.doi.org/10.3390/agronomy14092156.

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Biological nitrogen fixation by rhizobia-nodulated legumes reduces the dependence on synthetic nitrogen fertilizers. Identification of locally adapted rhizobia may uncover economically valuable strains for sustainable agriculture. This study investigated the diversity and symbiotic potential of rhizobia associated with three Medicago species from Eastern Romania’s ecosystems. Phenotypic screening ensured that only rhizobial species were retained for molecular characterization. 16S rDNA sequencing clustered the isolates into four distinct groups: Sinorhizobium meliloti, Sinorhizobium medicae, R
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Salem, N. M., F. Quaglino, A. Abdeen, et al. "First Report of ‘Candidatus Phytoplasma solani’ Strains Associated with Grapevine Bois Noir in Jordan." Plant Disease 97, no. 11 (2013): 1505. http://dx.doi.org/10.1094/pdis-04-13-0428-pdn.

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During a survey carried out in Jordanian vineyards in August and October 2012, grapevine (Vitis vinifera L.) plants showing typical grapevine yellows (GY) disease symptoms, including leaf discoloration and curling, berry shriveling, and irregular maturation of wood, were observed. In the same vineyards, bindweed (Convolvulus arvensis L.) plants showing stunting and leaf chromatic alteration were found, suggesting the involvement of phytoplasmas in the disease etiology. Using a CTAB method, total DNA was extracted from leaf veins of 25 symptomatic and two asymptomatic grapevines, and from five
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Quaglino, F., D. Maghradze, N. Chkhaidze, P. Casati, O. Failla, and P. A. Bianco. "First Report of ‘Candidatus Phytoplasma solani’ and ‘Ca. P. convolvuli’ Associated with Grapevine Bois Noir and Bindweed Yellows, Respectively, in Georgia." Plant Disease 98, no. 8 (2014): 1151. http://dx.doi.org/10.1094/pdis-01-14-0026-pdn.

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A survey carried out in Georgian vineyards, located in the Khaketi region, in September 2013, showed the presence of vines of the cultivar Chardonnay with typical grapevine yellows (GY) symptoms including leaf discoloration and curling, berry shriveling, and irregular maturation of wood. In the same vineyards, bindweed (Convolvulus arvensis L.) plants showing shoot proliferation and leaf yellowing were found, suggesting the involvement of phytoplasmas in the disease etiology. Total DNA was extracted by a CTAB method from leaf veins of 18 symptomatic and two asymptomatic grapevines, and from fo
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Holeva, M. C., P. E. Glynos, C. D. Karafla, E. M. Koutsioumari, K. B. Simoglou, and E. Eleftheriadis. "First Report of Candidatus Phytoplasma solani Associated with Potato Plants in Greece." Plant Disease 98, no. 12 (2014): 1739. http://dx.doi.org/10.1094/pdis-05-14-0488-pdn.

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In August 2013, potato plants (Solanum tuberosum) cv. Banba displaying symptoms resembling those caused by Candidatus Phytoplasma solani (potato stolbur phytoplasma) were observed in a 2-ha field in the area of the Peripheral Unit of Drama (northern Greece). The plants were 10 weeks old and their symptoms included reddening and upward rolling of leaflets, reduced size of leaves, shortened internodes, and aerial tuber formation. Incidence of affected plants was estimated to be 40% in the field. Four symptomatic potato plants were collected for laboratory testing of possible phytoplasma infectio
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Wanner, Leslie A. "A New Strain of Streptomyces Causing Common Scab in Potato." Plant Disease 91, no. 4 (2007): 352–59. http://dx.doi.org/10.1094/pdis-91-4-0352.

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Common scab is a serious disease of potatoes (Solanum tuberosum) and other root and tuber crops, affecting the quality and market value of these crops. The disease is caused by gram-positive soil bacteria in the genus Streptomyces. A new common scab-causing streptomycete was isolated from scabby potatoes originating in southeastern Idaho. Research has supported a model of horizontal transfer of pathogenicity determinants among streptomycetes, and the new strain has hallmarks of the recently characterized Streptomyces pathogenicity island (PAI); it has genes encoding the synthetase for the path
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Zhang, Yinjun, Jialin Chen, Changsheng Chen, and Shijin Wu. "Isolation of a Bacillus Aryabhattai Strain for the Resolution of (R, S)-Ethyl Indoline-2-Carboxylate to Produce (S)-Indoline-2-Carboxylic Acid." Catalysts 9, no. 2 (2019): 206. http://dx.doi.org/10.3390/catal9020206.

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The strain screened from sludge can selectively hydrolyze (S)-ethyl indoline-2-carboxylate to produce (S)-indoline-2-carboxylic acid. It was identified as the Bacillus aryabhattai strain based on its morphology, metabolism, and 16S rDNA sequence analysis. Glucose and yeast powder were used as the best carbon and nitrogen sources to cultured cells with an initial pH of seven. Subsequently, we optimized the key parameters for selective hydrolysis. Finally, when the substrate concentration had reached 3%, with a 35 °C water bath, a pH of seven, and a speed of 600 rpm, the e.e.p value attained 96%
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Crosslin, J. M., N. Olsen, and P. Nolte. "First Report of Zebra Chip Disease and “Candidatus Liberibacter solanacearum” on Potatoes in Idaho." Plant Disease 96, no. 3 (2012): 453. http://dx.doi.org/10.1094/pdis-10-11-0853.

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In September 2011, potato (Solanum tuberosum L.) tubers graded in a packing facility in south-central Idaho were observed with internal discolorations suggestive of zebra chip disease (ZC). Symptoms were observed in 1 to 2% of tubers of cv. Russet Norkotah and included brown spots and streaks especially in and near the vascular tissue. Some tubers also showed a dark and sunken stolon attachment typical of ZC (1). Initially, tissue samples were taken from seven symptomatic tubers and tested by PCR for “Candidatus Liberibacter solanacearum”, the bacterium associated with ZC. Primers specific for
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40

Heilig, Hans G. H. J., Erwin G. Zoetendal, Elaine E. Vaughan, Philippe Marteau, Antoon D. L. Akkermans, and Willem M. de Vos. "Molecular Diversity of Lactobacillus spp. and Other Lactic Acid Bacteria in the Human Intestine as Determined by Specific Amplification of 16S Ribosomal DNA." Applied and Environmental Microbiology 68, no. 1 (2002): 114–23. http://dx.doi.org/10.1128/aem.68.1.114-123.2002.

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ABSTRACT A Lactobacillus group-specific PCR primer, S-G-Lab-0677-a-A-17, was developed to selectively amplify 16S ribosomal DNA (rDNA) from lactobacilli and related lactic acid bacteria, including members of the genera Leuconostoc, Pediococcus, and Weissella. Amplicons generated by PCR from a variety of gastrointestinal (GI) tract samples, including those originating from feces and cecum, resulted predominantly in Lactobacillus-like sequences, of which ca. 28% were most similar to the 16S rDNA of Lactobacillus ruminis. Moreover, four sequences of Leuconostoc species were retrieved that, so far
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41

Thaenasa, Anisanee, Nakorn Pradit, Warayutt Pilap, et al. "Exploring the Genetic Diversity of the Jewel Beetles Sternocera aequisignata Saunders, 1866, and S. ruficornis Saunders, 1866 (Coleoptera: Buprestidae) in Thailand and Lao PDR." Insects 16, no. 3 (2025): 322. https://doi.org/10.3390/insects16030322.

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Jewel beetles of the genus Sternocera are widely distributed across tropical regions, including Thailand and Lao PDR, where the green-legged S. aequisignata Saunders, 1866, and red-legged S. ruficornis Saunders, 1866, are commonly found. These jewel beetles have significant economic importance, as they are edible, and their iridescent wings are used to create jewelry, leading to high market demand and intensive harvesting. Additionally, their habitats are being rapidly destroyed, resulting in population decline. However, genetic information on these species remains limited. This study aims to
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BİNİCİ, BURCU, DENİZ ŞİRİN, and MEHMET SAİT TAYLAN. "Molecular genetics and phylogeny of Ephippigera species group of genus Saga Charpentier, 1825 (Orthoptera: Ensifera: Saginae) in Anatolia." Zootaxa 4991, no. 2 (2021): 331–42. http://dx.doi.org/10.11646/zootaxa.4991.2.6.

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The genus Saga, one of the largest predatory bush crickets and insects in the Palearctic region, is represented by nine species in Anatolia. According to the morphological and bioacoustics evaluations of the Ephippigera species group belonging to the Saga genus, it consists of three species: S. ephippigera, S. syriaca, and S. hakkarica. To the present, however, there is no molecular genetic research on this species group. In our study, by using the 16S rDNA gene sequences, the molecular phylogeny of this species group is revealed and the morphological species hypotheses has been tested. In the
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43

Palero, Ferran, Guillermo Guerao, Paul F. Clark, and Pere Abelló. "Scyllarus arctus (Crustacea: Decapoda: Scyllaridae) final stage phyllosoma identified by DNA analysis, with morphological description." Journal of the Marine Biological Association of the United Kingdom 91, no. 2 (2010): 485–92. http://dx.doi.org/10.1017/s0025315410000287.

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Advanced stages of Scyllarus phyllosoma larvae were collected by demersal trawling during fishery research surveys in the western Mediterranean Sea in 2003–2005. Nucleotide sequence analysis of the mitochondrial 16S rDNA gene allowed the final-stage phyllosoma of Scyllarus arctus to be identified among these larvae. Its morphology is described and illustrated. This constitutes the second complete description of a Scyllaridae phyllosoma with its specific identity being validated by molecular techniques (the first was S. pygmaeus). These results also solved a long lasting taxonomic anomaly of se
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Ferreira, T., C. A. van Reenen, P. Tailliez, S. Pagès, A. P. Malan, and L. M. T. Dicks. "First report of the symbiotic bacterium Xenorhabdus indica associated with the entomopathogenic nematode Steinernema yirgalemense." Journal of Helminthology 90, no. 1 (2014): 108–12. http://dx.doi.org/10.1017/s0022149x14000583.

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AbstractThe entomopathogenic nematode Steinernema yirgalemense is considered a promising agent in the biocontrol of insects. However, little is known about the bacteria living in symbiosis with the nematode. In this study, we have identified the only available bacterial strain (157-C) isolated from S. yirgalemense, as a member of the species Xenorhabdus indica. Identification was based on 16S rDNA, recA, dnaN, gltX, gyrB and infB gene sequence analyses. The relatedness of strain 157-C to the type strain of X. indica (DSM 17 382) was confirmed with DNA–DNA hybridization. The phenotypic characte
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Tan, ZQ, R. Men, RY Zhang, and Z. Huang. "First Report of Herbaspirillum rubrisubalbicans Causing Mottled Stripe Disease on Sugarcane in China." Plant Disease 94, no. 3 (2010): 379. http://dx.doi.org/10.1094/pdis-94-3-0379b.

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Narrow, red stripes were observed on leaves and sheaths of sugarcane in 2007 in DanZhou County of Hainan Province and XuWen County of GuangDong Province, China. Stripes were parallel to the leaf veins. Some stripes were short (2 to 10 cm) and some were >1 m long, extending from the base of leaves. Width of the stripes was 2 to 4 mm. Symptoms varied with the cultivar. Cv. Taiwang 25, which was the most affected, exhibited red stripes and stalk death from the apex. Cvs. Taiwang 26 and Guang Dong 00236 were slightly affected with only red stripes. Symptoms on cv. Taiwang 22 were mottled stripe
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Nedashkovskaya, Olga I., Makoto Suzuki, Marc Vancanneyt, et al. "Salegentibacter holothuriorum sp. nov., isolated from the edible holothurian Apostichopus japonicus." International Journal of Systematic and Evolutionary Microbiology 54, no. 4 (2004): 1107–10. http://dx.doi.org/10.1099/ijs.0.02987-0.

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Strain KMM 3524T was isolated from the holothurian Apostichopus japonicus living in the Sea of Japan. The bacterial strain was pigmented, non-motile, Gram-negative, strictly aerobic and oxidase-, catalase- and β-galactosidase-positive. From the results of 16S rDNA sequence analysis, strain KMM 3524T was found to be related closely to Salegentibacter salegens (98·1 %). DNA–DNA homology between strains KMM 3524T and S. salegens DSM 5424T was 38 %; this showed clearly that the holothurian isolate KMM 3524T belongs to a novel species of the genus Salegentibacter for which the name Salegentibacter
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47

Bukhalid, R. A., T. Takeuchi, D. Labeda, and R. Loria. "Horizontal Transfer of the Plant Virulence Gene, nec1, and Flanking Sequences among Genetically Distinct Streptomyces Strains in the Diastatochromogenes Cluster." Applied and Environmental Microbiology 68, no. 2 (2002): 738–44. http://dx.doi.org/10.1128/aem.68.2.738-744.2002.

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ABSTRACT Evidence for the horizontal transfer of a pathogenicity island (PAI) carrying the virulence gene nec1 and flanking sequences among Streptomyces strains in the Diastatochromogenes cluster is presented. Plant-pathogenic, thaxtomin-producing Streptomyces strains, previously classified as S. scabiei based on the conventionally used phenotypic characteristics, were found to be genetically distinct from the type strain of S. scabiei based on DNA relatedness and 16S rDNA sequence analysis. Pairwise DNA-DNA hybridizations between some of these strains and the S. scabiei type strain were as lo
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48

Ohike, Tatsuya, Tetsuya Matsukawa, Masahiro Okanami, Shin’ichiro Kajiyama, and Takashi Ano. "Biological Control Potential of Streptomyces sp. AR10 Producing Albocycline Isolated from Soil around Ant Nest." Journal of Agricultural Science 10, no. 3 (2018): 54. http://dx.doi.org/10.5539/jas.v10n3p54.

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Fifty actinomycetes were isolated from fifteen soil samples and were screened for their antagonism against fungal plant pathogens by dual culture assay, and one of the strain named AR10 was shown to be most effective in suppression of growth of plant pathogen. An antifungal compound of AR10 was extracted, and purified by TLC and HPLC. As a result of NMR and LC-MS analysis, the antifungal compound was identified as albocycline. AR10 suppressed Rhizoctonia damping-off of cucumber in infection control assay. The 16S rDNA sequence of AR10 shows high sequence similarity to those of genus Streptomyc
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Inci, Tuney-Kizilkaya, and Grace Tunka Bengil Elizabeth. "Identification complexity of critically endangered Squatina squatina (Linnaeus, 1758) and Squatina aculeata Cuvier, 1829 in the Mediterranean Sea (Turkey)." Journal of Wildlife and Biodiversity 6, no. X (2022): X. https://doi.org/10.5281/zenodo.6522467.

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Sharks and rays, besides their economic importance, are ecologically important organisms with a diverse group. After a pregnant <em>Squatina</em> sp. was captured as by-catch, Akyol et al. (2015) published this species as <em>Squatina squatina</em> while the COI and 16S rDNA analysis demonstrated that the species is <em>S. aculeata</em>. This complexity revealed that utilizing morphologic identification solely might not be enough to distinguish these two <em>Squatina</em> species accurately. We aim to compare morphologic and molecular techniques during species identification of critically enda
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Gómez-Valero, Laura, Mario Soriano-Navarro, Vicente Pérez-Brocal, et al. "Coexistence of Wolbachia with Buchnera aphidicola and a Secondary Symbiont in the Aphid Cinara cedri." Journal of Bacteriology 186, no. 19 (2004): 6626–33. http://dx.doi.org/10.1128/jb.186.19.6626-6633.2004.

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ABSTRACT Intracellular symbiosis is very common in the insect world. For the aphid Cinara cedri, we have identified by electron microscopy three symbiotic bacteria that can be characterized by their different sizes, morphologies, and electrodensities. PCR amplification and sequencing of the 16S ribosomal DNA (rDNA) genes showed that, in addition to harboring Buchnera aphidicola, the primary endosymbiont of aphids, C. cedri harbors a secondary symbiont (S symbiont) that was previously found to be associated with aphids (PASS, or R type) and an α-proteobacterium that belongs to the Wolbachia gen
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