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Artykuły w czasopismach na temat "2D-PAGE"

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Matera, Robert, Katalin V. Horvath, Hari Nair, Ernst J. Schaefer, and Bela F. Asztalos. "HDL Particle Measurement: Comparison of 5 Methods." Clinical Chemistry 64, no. 3 (2018): 492–500. http://dx.doi.org/10.1373/clinchem.2017.277632.

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Abstract BACKGROUND HDL cell cholesterol efflux capacity has been documented as superior to HDL cholesterol (HDL-C) in predicting cardiovascular disease risk. HDL functions relate to its composition. Compositional assays are easier to perform and standardize than functional tests and are more practical for routine testing. Our goal was to compare measurements of HDL particles by 5 different separation methods. METHODS HDL subfractions were measured in 98 samples using vertical auto profiling (VAP), ion mobility (IM), nuclear magnetic resonance (NMR), native 2-dimensional gel electrophoresis (2
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Chorney, M. J., J. S. Tung, Y. Bushkin, and F. W. Shen. "Structural characteristics of Tla products." Journal of Experimental Medicine 162, no. 3 (1985): 781–89. http://dx.doi.org/10.1084/jem.162.3.781.

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Biochemical study of thymus leukemia antigen (TL) from thymocytes of various Tla genotypes and from leukemia cells revealed features that, given present evidence, are peculiar to TL among class I products of the H-2:Qa:Tla region of chromosome 17. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of TL from thymocytes of all TL+ mouse strains, precipitated by anti-TL antiserum or monoclonal antibodies, showed two closely migrating bands of equal intensity in the heavy (H) chain position (45-50,000 mol wt). Comparison of these two bands by two-dimensional isoelectric focusing
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Hanneken, Marina, Doreen Ackermann, Simone König, and Günter Thesseling. "Koordinatenbestimmung von Proteinpunkten in 2D-PAGE-Gelen." BIOspektrum 20, no. 6 (2014): 655–57. http://dx.doi.org/10.1007/s12268-014-0503-5.

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Vijayendran, Chandran, Sebastian Burgemeister, Karl Friehs, Karsten Niehaus, and Erwin Flaschel. "2DBase: 2D-PAGE database of Escherichia coli." Biochemical and Biophysical Research Communications 363, no. 3 (2007): 822–27. http://dx.doi.org/10.1016/j.bbrc.2007.09.050.

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Pietrogrande, Maria Chiara, Nicola Marchetti, Francesco Dondi, and Pier Giorgio Righetti. "Decoding 2D-PAGE complex maps: Relevance to proteomics☆." Journal of Chromatography B 833, no. 1 (2006): 51–62. http://dx.doi.org/10.1016/j.jchromb.2005.12.051.

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Griebel, Anja, Christian Obermaier, Reiner Westermeier, Martin Moche, and Knut Büttner. "Fluoreszenzmarkierung von Proteinen für 1D- und 2D-PAGE." BIOspektrum 19, no. 6 (2013): 653–55. http://dx.doi.org/10.1007/s12268-013-0375-0.

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Li, Feng, Françoise Seillier-Moiseiwitsch, and Valeriy R. Korostyshevskiy. "Region-based statistical analysis of 2D PAGE images." Computational Statistics & Data Analysis 55, no. 11 (2011): 3059–72. http://dx.doi.org/10.1016/j.csda.2011.05.013.

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Sutton, C. A., M. W. Shirley, and M. H. Wisher. "Characterization of coccidial proteins by two-dimensional sodium dodecyl sulphate–polyacrylamide gel electrophoresis." Parasitology 99, no. 2 (1989): 175–87. http://dx.doi.org/10.1017/s0031182000058613.

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SummaryTwo dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (2D SDS–PAGE) has been used to produce ‘fingerprint’ maps of the proteins from each of the 7 species of Eimeria which infect the chicken. All 7 species could be identified from their array of polypeptides but few differences were detected between strains of the same species. Alterations to the polypeptide array associated with the stage of sporulation of the oocysts were observed. lodination of sporozoites, 2D SDS–PAGE, autoradiography and immunoblotting techniques were combined to identify polypeptides with a su
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Pavlicevic, Milica, Sladjana Stanojevic, and Biljana Vucelic-Radovic. "Influence of extraction method on protein profile of soybeans." Chemical Industry 67, no. 4 (2013): 687–94. http://dx.doi.org/10.2298/hemind120919115p.

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Comparison between protein profiles of soybean obtained by commonly used methods of extraction (Tris buffer and Tris-urea buffer) with methods used for extraction of plant proteins for 2D PAGE analysis (direct solubilization in IEF buffer, acetone extraction, phenol extraction, extraction with urea solubilization buffer and thiourea-urea extraction) was investigated. 2D profiles of samples extracted directly in IEF buffer, in urea solubilization buffer and in acetone were characterized with low number of spots. Analysis of 2D PAGE profiles of Tris buffer and Tris-urea buffer extracts showed hi
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Tao, R., H. Yamane, H. Sassa, et al. "Stylar Proteins Associated with Gametophytic Self-incompatibility in the Prunus." HortScience 32, no. 3 (1997): 514E—514. http://dx.doi.org/10.21273/hortsci.32.3.514e.

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Stylar proteins of four Prunus species, P. avium, P. dulcis, P. mume, and P. salicina, were surveyed by 2D-PAGE combined with immunoblot and N-terminal amino acid sequence analyses to identify S-proteins associated with gametophytic SI in the Prunus. All four S-allelic products tested for P. dulcis could be identified in the highly basic zone of the gel. These S-proteins had Mr of about 28–30 kDa and reacted with the anti-S4-serum prepared from Japanese pear (Pyrus serotina). Two of six S-allelic products tested for P. avium could be also identified in the 2D-PAGE profiles, with roughly the sa
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Rozprawy doktorskie na temat "2D-PAGE"

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Brown, Andrew S. "Two-dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) Characterization of Decorin." Youngstown State University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1311873768.

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Queiroz, João Vitor de. "Estratégias bioanalíticas para caracterização de biomarcadores de exposição ao mercúrio em Arapaima gigas e Serrasalmus rhombeus do rio Madeira/bacia amazônica." Botucatu, 2017. http://hdl.handle.net/11449/150276.

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Orientador: Pedro de Magalhães Padilha<br>Resumo: Este trabalho apresenta os resultados de proteínas associadas ao mercúrio em amostras de tecido muscular e hepático de pirarucu (Arapaima gigas) e piranha preta (Serrasalmus rhombeus) oriundos do complexo hidrelétrico de Jirau, na Bacia do Rio Madeira, região amazônica do Brasil. O proteôma do músculo e fígado dessas espécies foi obtido por eletroforese bidimensional em gel de poliacrilamida (2D PAGE). O mercúrio presente nos spots proteicos foi determinado por espectrometria de absorção atômica em forno de grafite (GFAAS) após mineralização ác
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Cerbino, Maria Rosa. "ABORDAGEM METALOPROTEÔMICA DO MERCÚRIO EM LEITE MATERNO DE COMUNIDADES DA BACIA AMAZÔNICA - BRASIL." Pontifícia Universidade Católica de Goiás, 2016. http://tede2.pucgoias.edu.br:8080/handle/tede/3556.

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Submitted by admin tede (tede@pucgoias.edu.br) on 2016-11-30T18:10:51Z No. of bitstreams: 1 MARIA ROSA CERBINO.pdf: 2627014 bytes, checksum: 98680184bb60e636de01507e3f1eeec4 (MD5)<br>Made available in DSpace on 2016-11-30T18:10:51Z (GMT). No. of bitstreams: 1 MARIA ROSA CERBINO.pdf: 2627014 bytes, checksum: 98680184bb60e636de01507e3f1eeec4 (MD5) Previous issue date: 2016-04-12<br>Mercury is a potentially toxic element with a wide distribution on the Amazonian environment. This metal is dangerous and responsible for environmental contaminations and human intoxications as it is capable to bi
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Nadaf, Somayyeh. "Analyse protéomique et transcriptomique de la maturation folliculaire." Thesis, Tours, 2010. http://www.theses.fr/2010TOUR4037.

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La compréhension des mécanismes cellulaires et moléculaires qui sont mis en jeu au moment de la croissance et la maturation pré-ovulatoire induite par la LH, permettra de définir des marqueurs de qualité et de maturité du follicule destiné à ovuler, et ainsi de mieux anticiper le moment de l’ovulation. L’objectif majeur de cette thèse était d’identifier certain des facteurs régulateur impliqués dans la maturation folliculaire par deux approches globales d’analyse protéomique et transcriptomique. La première étude a permis d’établir pour la première fois les cartes protéiques du liquide follicu
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Fulton, Benjamin L. "2D-PAGE Analysis of Myocardial Collagen in Male and Female Spontaneously Hypertensive Rats." Connect to resource online, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1219668882.

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Schmitz, Gabriela Justamante Handel. "Análise comparativa dos proteomas das raízes tuberosas de mandioca (Manihot esculenta Crantz) de variedades de mesa e indústria." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/9/9131/tde-28082015-154641/.

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A mandioca (Manihot esculenta Crantz) é uma das principais culturas do mundo, havendo grande variabilidade genética. As variedades são classificadas com base na palatabilidade e toxicidade das raízes, em mansas ou doces e bravas ou amargas. Apesar da importância, o potencial da mandioca é pouco explorado, não sendo conhecidos, em nível molecular, os elementos determinantes para as suas características. Assim, pretendeu-se identificar, empregando a 2D-PAGE, proteínas que possam estar associadas com as diferenças físico-químicas das raízes tuberosas de variedades de mesa (IAC 576-70 e IAC 06-01)
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MILLI, Alberto. "2D-page coupled to mass spectrometry for proteomic analysis of human, microbial and plant samples." Doctoral thesis, Università degli Studi di Verona, 2009. http://hdl.handle.net/11562/337513.

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Il progetto di dottorato è stato condotto nel Laboratorio di Proteomica del Dipartimento di Biotecnologie dell’Università di Verona. Per la stesura di tale progetto sono state instaurate collaborazioni con alcuni laboratori, sia interni allo stesso Dipartimento sia appartenenti ad altre Università o Istituti di Ricerca. In particolare, per quanto riguarda lo studio su cellule di cancro colorettale trattate con un nuovo chemiofarmaco, il Laboratorio del Prof. Zunino (Fondazione IRCCS, Istituto Nazionale dei Tumori, Milano) ha fornito tutti i campioni biologici, mentre il gruppo del Prof.
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Vieira, José Cavalcante Souza. "Investigação de metalotioneínas em peixes da região de Jirau - bacia do Rio Madeira - Rondônia." Botucatu, 2017. http://hdl.handle.net/11449/150736.

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Orientador: Pedro de Magalhães Padilha<br>Resumo: Devido a sua grande concentração de nutrientes, tais como proteínas, vitaminas e minerais, o peixe é considerado um dos alimentos mais saudáveis que se pode encontrar na natureza. No entanto, a ingestão de peixes é considerada a forma predominante de via de exposição do ser humano ao mercúrio (Hg), principalmente para as populações que vivem às margens dos rios, onde o peixe constitui a principal fonte de proteína. Na tentativa de elucidar os mecanismos de toxicidade das espécies mercuriais, o teor desse metal tem sido estudado intensamente pel
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Lonardoni, Francesco. "Discovery and quantification of proteins of biological relevance through differential proteomics and biosensing." Thesis, Cranfield University, 2012. http://dspace.lib.cranfield.ac.uk/handle/1826/8676.

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Medical diagnosis is the process of attempting to determine and/or identify a possible disease or disorder. This process is revealed by biomarkers, defined by The Food and Drug Administration (FDA) as “characteristics that are objectively measured and evaluated as indicators of normal biologic processes, pathogenic processes, or pharmacologic responses to a therapeutic intervention”. The process of biomarker discovery has been boosted in the last years by proteomics, a research discipline that takes a snapshot of the entire wealth of proteins in an organism/ tissue/ cell/ body fluid. An implem
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Mabiya, Thembeka. "Development of a plum chromosome doubling method and proteomics and biochemical characterization." University of the Western Cape, 2015. http://hdl.handle.net/11394/4875.

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>Magister Scientiae - MSc<br>Chromosome doubling has become an important tool in breeding programmes as it offers the ability of introducing novel traits into existing plants. Doubled haploid plants are highly valued by both consumers and breeders as these plants usually show larger flower, leaves and fruit, thus making them more marketable. Marianna open pollinated plum rootstocks’ adaptability to different soil types and moisture conditions has been favoured in polyploidy studies as parental material in breeding programmes. The potential of the microtubule depolymerizing herbicide (oryzalin)
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Książki na temat "2D-PAGE"

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Posch, Anton, ed. 2D PAGE: Sample Preparation and Fractionation. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-064-9.

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Posch, Anton, ed. 2D PAGE: Sample Preparation and Fractionation. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-210-0.

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Anton, Posch, ed. 2D PAGE: Sample preparation and fractionation. Humana Press, 2008.

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Reichert, Gerd Hermann. Autosomale Trisomien bei Maus und Mensch, Untersuchung der Proteinmuster durch IEF-2D-PAGE [IEF-D-PAGE]. [s.n.], 1985.

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2D PAGE : Sample Preparation and Fractionation: Volume 2. Humana Press, 2008.

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Posch, Anton. 2D PAGE : Sample Preparation and Fractionation: Volume 1. Humana Press, 2010.

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Posch, Anton. 2D PAGE : Sample Preparation and Fractionation: Volume 2. Humana Press, 2010.

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2D PAGE: Sample Preparation and Fractionation: Volume 1 (Methods in Molecular Biology). Humana Press, 2008.

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Posch, Anton. 2D PAGE: Sample Preparation and Fractionation: Volume 2 (Methods in Molecular Biology). Humana Press, 2008.

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Denzler, Regina. Darstellung der Proteinexpression in NG 108-15 Hybridzellen nach akuter und chronischer Morphinbehandlung unter Nutzung der zweidimensionalen Polyacrylamid-Gelelektrophorese (2D-PAGE). 2000.

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Części książek na temat "2D-PAGE"

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Marengo, Emilio, Elisa Robotti, and Marco Bobba. "2D-PAGE Maps Analysis." In Methods in Molecular Biology™. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-117-8_16.

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Robotti, Elisa, Elisa Calà, and Emilio Marengo. "for 2D-PAGE Data Analysis." In Methods in Molecular Biology. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1641-3_2.

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May, Caroline, Frederic Brosseron, Kathy Pfeiffer, Helmut E. Meyer, and Katrin Marcus. "Proteome Analysis with Classical 2D-PAGE." In Methods in Molecular Biology. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-885-6_3.

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May, Caroline, Frederic Brosseron, Kathy Pfeiffer, et al. "Proteome Analysis with Classical 2D-PAGE." In Methods in Molecular Biology. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1024-4_5.

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Maaß, Sandra. "Absolute Protein Quantification Using AQUA-Calibrated 2D-PAGE." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8695-8_11.

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Le, Kim Phuong Uyen, Phuong Uyen Vo, Kieu Minh Le, et al. "2D-Page Analysis of Vietnamese Colorectal Cancer Tissue Samples." In 6th International Conference on the Development of Biomedical Engineering in Vietnam (BME6). Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-4361-1_48.

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Bitrián, Marta, Antonio F. Tiburcio, and Rubén Alcázar. "Determination of Posttranslational Modifications by 2D PAGE: Applications to Polyamines." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7398-9_28.

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Álvaro, Francisco, Francisco Cruz, Joan-Andreu Sánchez, Oriol Ramos Terrades, and José-Miguel Benedí. "Page Segmentation of Structured Documents Using 2D Stochastic Context-Free Grammars." In Pattern Recognition and Image Analysis. Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-38628-2_15.

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Green, George R., and Duc P. Do. "Purification and Analysis of Variant and Modified Histones Using 2D PAGE." In The Nucleus. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-461-6_16.

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Marengo, Emilio, and Elisa Robotti. "A New Algorithm for the Simulation of SDS 2D-PAGE Datasets." In Methods in Molecular Biology. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-821-4_34.

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Streszczenia konferencji na temat "2D-PAGE"

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Wang, Mark M., Frederick B. McCormick, and Sadik C. Esener. "2D and 3D Equalizers for Page-Oriented Optical Memories." In Optics in Computing. Optica Publishing Group, 1997. http://dx.doi.org/10.1364/oc.1997.owd.4.

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In this study we investigate the use of 2D and 3D equalizers to suppress intersymbol interference (ISI) in 2D parallel channels. The techniques demonstrated here are best suited for parallel implementation and can be used for any page-oriented readout application including increasing the data density achievable in volume optical memories or increasing the number of links achievable in free-space optical interconnects. Page-oriented optical memories are currently being investigated for their potential as high data-rate, high capacity storage devices,1-3 however the cost-effectiveness of such de
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"Page Analysis by 2D Conditional Random Fields." In International Conference on Pattern Recognition Applications and Methods. SciTePress - Science and and Technology Publications, 2013. http://dx.doi.org/10.5220/0004266505640567.

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Neifeld, Mark A. "2D Coding and Signal Processing for Volume Memory Interfaces." In Optics in Computing. Optica Publishing Group, 1997. http://dx.doi.org/10.1364/oc.1997.owd.1.

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Volume optical memory represents a unique type of storage channel. It can be characterized as a 2D parallel, space-variant system with potentially time-varying characteristics. Like any other storage channel however, imperfections and noise degrade the SNR of retrieved data and this degradation may result in data errors. As with traditional memory and communications systems, detection and error handling techniques can be used to recover lost SNR and correct data errors.[1,2] The success of detection theoretic techniques is based on the use of prior knowledge concerning channel characteristics
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Latib, Norhidayu Abdul, Safida Anira Norshaha, Gires Usup, and Nurul Yuziana Mohd Yusof. "2D-PAGE protein analysis of dinoflagellate Alexandrium minutum based on three different temperatures." In THE 2015 UKM FST POSTGRADUATE COLLOQUIUM: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. AIP Publishing LLC, 2015. http://dx.doi.org/10.1063/1.4931248.

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"2D-PAGE Texture Classification using Support Vector Machines and Genetic Algorithms - An Hybrid Approach for Texture Image Analysis." In International Conference on Bioinformatics Models, Methods and Algorithms. SciTePress - Science and and Technology Publications, 2013. http://dx.doi.org/10.5220/0004187400050014.

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Chen, C. H., B. Hoanca, C. B. Kuznia, J. M. Wu, and A. A. Sawchuk. "Smart Pixel Array Network Interface (SAPIENT) for 2D Parallel Data Packet Networks." In Optics in Computing. Optica Publishing Group, 1997. http://dx.doi.org/10.1364/oc.1997.othd.11.

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We describe a SmArt PIxEl for NeTwork Interface (SAPIENT) chip that performs interfacing of processors to 2-D parallel optical free space networks. This network transfers 2-D parallel data packets between processors on digital optical channels. We assume these parallel data packets contain address information for the destination processor, similar to the format of an ATM packet network, except the packets are passed between processors in a parallel format, in a single clock cycle. Each SAPIENT chip is capable of checking the address bits of an incoming parallel data packet, re-transmitting (or
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Evjenth, Andreas, Otto Andreas Moe, Iselin Violet Kjelland Schøn, and Thomas J. Impelluso. "A Dynamic Model for Motion of an ROV due to On-Board Robotics." In ASME 2017 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2017. http://dx.doi.org/10.1115/imece2017-70110.

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A new method in dynamics — the Moving Frame Method (MFM) — is used to conduct the analysis of how a robotic appendage (manipulator) on a Remotely Operated Vehicle (ROV) affects the motion of the ROV. An ROV performs multiple tasks on the seabed in the oil service industry. In most cases, an ROV pilot monitors and adjusts the movement of the vehicle due to induced motion by currents, buoyancy and the manipulators. Simulation data would assist the pilot and improve the stability of the ROV. This paper exploits a new method to analyze the induced movements of the ROV. The method uses the Special
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Devanathan, Srikanth, and Karthik Ramani. "Towards Enabling Visual Design Exploration Involving Multiple Abstractions of Design Descriptions." In ASME 2011 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. ASMEDC, 2011. http://dx.doi.org/10.1115/detc2011-47291.

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Designers use several visual tools for exploring and understanding design problems and solutions. House of Quality (HoQ), function-structure, Morphological matrices, concept selection tables, 2D drawings are some of the visual tools and representations used in mechanical design. In this article we attempt to connect these visual tools and their underlying models to support exploration in early design using a representation called the working knowledge model (WKM). We identify two key aspects in design that are important for establishing such connections: different abstractions are used to desc
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Hassan, Mohamed Ali, Jim Strand, and Per Inge Remmen. "Cloud-Based Software for Automated Well Planning." In Gas & Oil Technology Showcase and Conference. SPE, 2023. http://dx.doi.org/10.2118/214075-ms.

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Abstract The Automatic Well Design workflow presented in this paper is a starting step to achieve Well Construction Autonomy. This workflow made possible through modern "API" based drilling calculation engines hosted on a cloud computing environment. In the Drilling Digitalization Infrastructure, everything will be connected via Open APIs, and Real time Machine-to- Machine Communication. Modern Cloud-Based Applications will enable Engineers, within seconds, to automatically execute complex drilling simulations and calculations This software tool, named WellDesign, is a cloud based single page
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Mutsvanga, C., T. Zvarivadza, A. C. Adoko, and M. Onifade. "Numerical Modeling Application in the Design of Regional Pillars for an Ore-Replacement Project: Underground Platinum Mining Case Study." In 57th U.S. Rock Mechanics/Geomechanics Symposium. ARMA, 2023. http://dx.doi.org/10.56952/arma-2023-0795.

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ABSTRACT Pillars are critical to the stability and profitability of mining operations and their proper design is essential in ensuring that these objectives are met. A number of tools are available in designing pillars and numerical modelling is one of those tools. It is important to note that numerical modelling is not design in its self as wrongly assumed by many, but a tool that is used to aid the design process. This paper evaluates numerical modelling application in the design of regional pillars for an ore-replacement project, underground platinum mining case study, using three different
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Raporty organizacyjne na temat "2D-PAGE"

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Michalski, A,, D. Andersson, R. Rossi, and C. Soriano. D7.1 DELIVERY OF GEOMETRY AND COMPUTATIONAL MODEL. Scipedia, 2021. http://dx.doi.org/10.23967/exaqute.2021.2.020.

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This document describes the industrial application, on which the developments of the project are implemented, and the CFD set-up. The developments are implemented over six analysis cases with increasing complexity starting from a 2D geometry with mean wind inflow to a 3D geometry with turbulent inflow and real-time shape optimization. The application represents the CAARC tall building model, which has served as a benchmark model for many studies since the 1970’s when it was first developed. Base moments (bending and torsional moments) of the building are extracted for validation by comparison
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Badia, S., A. Martín, J. Principe, C. Soriano, and R. Rossi. D3.1 Report on nonlinear domain decomposition preconditioners and release of the solvers. Scipedia, 2021. http://dx.doi.org/10.23967/exaqute.2021.2.021.

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This document describes the industrial application, on which the developments of the project are implemented, and the CFD set-up. The developments are implemented over six analysis cases with increasing complexity starting from a 2D geometry with mean wind inflow to a 3D geometry with turbulent inflow and real-time shape optimization. The application represents the CAARC tall building model, which has served as a benchmark model for many studies since the 1970’s when it was first developed. Base moments (bending and torsional moments) of the building are extracted for validation by comparison
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