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1

Winstanley, Craig, Stephen B. Kaye, Timothy J. Neal, Helen J. Chilton, Silvia Miksch, and C. Anthony Hart. "Genotypic and phenotypic characteristics of Pseudomonas aeruginosa isolates associated with ulcerative keratitis." Journal of Medical Microbiology 54, no. 6 (2005): 519–26. http://dx.doi.org/10.1099/jmm.0.46005-0.

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Ghorbani, Ghasem, Ebrahim Rahimi, and Amir Shakerian. "Antibiotic resistance’s Genotypic and Phenotypic Characteristics and the Frequency of Virulence Factors in P. aeruginosa Isolates Isolated from Water Samples in Iran." BioMed Research International 2022 (September 30, 2022): 1–7. http://dx.doi.org/10.1155/2022/7076433.

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Pseudomonas aeruginosa is a pathogenic bacterium that can contaminate water. In this study, 430 water samples were evaluated for P. aeruginosa, antibiotic resistance, and the abundance of virulence factors. P. aeruginosa was isolated from 28 (6.51%) water samples. Among the types of water, well and spring water showed the highest P. aeruginosa with, respectively, 20 (15.6%) and 5 (8.06%) positive samples per type of samples. Drinking water and mineral water showed minor contamination with P. aeruginosa. The prevalence of antibiotic resistance against meropenem, imipenem, erythromycin, gentamic
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M. Ismail, Yasser, Sahar M. Fayed, Fatma M. Elesawy, Nora Z Abd El-Halim, and Ola S. El-Shimi. "Phenotypic and Molecular Characteristics of Pseudomonas Aeruginosa Isolated from Burn Unit." EJMM-Volume 30-Issue 1 30, no. 1 (2021): 19–28. http://dx.doi.org/10.51429/ejmm30103.

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Background: The biggest concern for a burn team is a nosocomial infection in burn patients, which is a significant health issue. Pseudomonas aeruginosa is an extremely troublesome drug-resistant bacterium in the world today. We are now faced with rising P. aeruginosa pan-drug-resistant clones in hospital settings. Objectives: To evaluate the distribution of different virulence factors generated by P. aeruginosa isolated from burn wound infections, together with its antimicrobial susceptibility. Methodology: The isolates reported as P. aeruginosa were further tested for the presence of various
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Winstanley, C., J. L. Fothergill, S. Panagea, C. H. M. Smart, M. J. Walshaw, and C. A. Hart. "71 Phenotypic and genotypic characteristics of a Cystic Fibrosis epidemic strain of Pseudomonas aeruginosa." Journal of Cystic Fibrosis 5 (2006): S16. http://dx.doi.org/10.1016/s1569-1993(06)80062-7.

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Koutsogiannou, M., E. Drougka, A. Liakopoulos, et al. "Comparative epidemiological study of Pseudomonas aeruginosa isolates during two time periods in a tertiary care hospital." ACTA MICROBIOLOGICA HELLENICA 59, no. 4 (2014): 43–56. https://doi.org/10.5281/zenodo.10017365.

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Pseudomonas aeruginosa is a nosocomial pathogen of clinical importance carrying virulence and antibiotic resistance determinants. In the present study, phenotypic and genotypic characteristics of P. aeruginosa isolates collected at the University General Hospital of Patras (UGHP) in two time periods were investigated.Two groups of P. aeruginosa clinical isolates were compared collected with one year interval, in the UGHP. Group A consisted of 120 isolates recovered during one year (2004), whereas, group B included 240 recovered during a two year period (2006-2007). P. aeruginosa isolates were
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Pourakbari, B., Z. Movahedi, S. Mahmoudi, et al. "Genotypic characteristics of Pseudomonas aeruginosa strains circulating in the tertiary referral Children's Medical Hospital in Tehran, Iran." British Journal of Biomedical Science 69, no. 4 (2012): 169–72. http://dx.doi.org/10.1080/09674845.2012.12069147.

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Miranda, C. C., I. de Filippis, L. H. Pinto, et al. "Genotypic characteristics of multidrug-resistant Pseudomonas aeruginosa from hospital wastewater treatment plant in Rio de Janeiro, Brazil." Journal of Applied Microbiology 118, no. 6 (2015): 1276–86. http://dx.doi.org/10.1111/jam.12792.

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McMullen, Allison R., Melanie L. Yarbrough, Meghan A. Wallace, Angela Shupe, and Carey-Ann D. Burnham. "Evaluation of Genotypic and Phenotypic Methods to Detect Carbapenemase Production in Gram-Negative Bacilli." Clinical Chemistry 63, no. 3 (2017): 723–30. http://dx.doi.org/10.1373/clinchem.2016.264804.

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Abstract BACKGROUND Carbapenemase-producing gram-negative bacteria (CP-GNB) are an urgent and expanding public health threat. Rapid and accurate identification of these organisms facilitates infection prevention efforts in healthcare facilities. The objective of our study was to evaluate methods to detect and identify CP-GNB. METHODS We examined 189 carbapenem-resistant GNB(CR-GNB), including Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii complex, using 3 different methods: 2 methods to screen isolates of GNB for carbapenemase production [the carbapenem inactivation me
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Borisova, Dayana, Tanya Strateva, Svetoslav G. Dimov, et al. "Diversification of Pseudomonas aeruginosa After Inhaled Tobramycin Therapy of Cystic Fibrosis Patients: Genotypic and Phenotypic Characteristics of Paired Pre- and Post-Treatment Isolates." Microorganisms 13, no. 4 (2025): 730. https://doi.org/10.3390/microorganisms13040730.

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This study examines the impact of inhaled tobramycin therapy on the within-host changes in P. aeruginosa strains isolated from Bulgarian patients with CF prior to and post treatment. Genotypic comparison by RAPD-PCR indicated that most of the pre-treatment isolates had a high similarity and were genetically comparatively close to strains from other countries with known increased morbidity or treatment requirements. Most of the post-treatment isolates were, however, genetically distant from their pre-treatment counterparts, showing genotypic diversification after the treatment. Phenotypic compa
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Bobai, Mathew, Lawal Danjuma, Nura Muhammad Sani, Joshua Istifanus Anekoson, and Yusuf Nuhu. "Correlation Between Patients with Wounds and Isolated Pseudomonas aeruginosa and Staphylococcus aureus at Barau Dikko Teaching Hospital, Kaduna, Nigeria." Journal of Biochemistry, Microbiology and Biotechnology 10, no. 2 (2022): 35–41. http://dx.doi.org/10.54987/jobimb.v10i2.755.

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This study was carried out to determine the correlation between patients with wounds and isolated Pseudomonas aeruginosa and Staphylococcus aureus in clinics at Barau Dikko Teaching Hospital, Kaduna, Nigeria. The Socio-demographic, medical and drug histories and characteristics of the wounds from each consented patient were taken using a questionnaire along with sixty samples of the patient’s wound swab samples. Isolation of Pseudomonas aeruginosa and Staphylococcus aureus from the wound swab samples was carried out using standard phenotypic and genotypic procedures. Out of the 60 samples coll
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Lukyanenko, N. V., K. I. Sursyakova, T. V. Safyanova, V. V. Prokopyev, and A. A. Russkikh. "Comparative Aspects of Phenotypic and Genotypic Characteristics of Strains of ISMP Pathogens Isolated from Patients of a Large Multidisciplinary Hospital." Medicina 13, no. 1 (2025): 33–46. https://doi.org/10.29234/2308-9113-2024-13-1-33-46.

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Introduction: In recent decades, Russia, like the rest of the world, has seen a rapid increase in antimicrobial resistance (AMR) among pathogens causing infectious diseases. The development of drug resistance leads to microorganisms' ability to survive despite the use of etiotropic therapy. Alongside multidrug resistance (MDR), hypervirulence – associated with the acquisition of additional genetic material and the emergence of new genetic lineages – is becoming increasingly significant. The purpose of the study: To conduct a comparative phenotypic and genotypic assessment of the main pathogens
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Qin, Xuan, Danielle M. Zerr, Michael A. McNutt, Jessica E. Berry, Jane L. Burns, and Raj P. Kapur. "Pseudomonas aeruginosa Syntrophy in Chronically Colonized Airways of Cystic Fibrosis Patients." Antimicrobial Agents and Chemotherapy 56, no. 11 (2012): 5971–81. http://dx.doi.org/10.1128/aac.01371-12.

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ABSTRACTPseudomonas aeruginosaisolates from cystic fibrosis (CF) patients undergo remarkable phenotypic divergence over time, including loss of pigmentation, hemolysis, motility, and quorum sensing and emergence of antibiotic hypersusceptibility and/or auxotrophism. With prolonged antibiotic treatment and steady decline in lung function in chronically infected patients, the divergent characteristics associated with CF isolates have traditionally been regarded as “adapted/unusual virulence,” despite the degenerative nature of these adaptations. We examined the phenotypic and genotypic diversity
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Milojković, Marko, Željka Nenadović, Slaviša Stanković, et al. "Phenotypic and genetic properties of susceptible and multidrug-resistant Pseudomonas aeruginosa isolates in Southern Serbia." Archives of Industrial Hygiene and Toxicology 71, no. 3 (2020): 231–50. http://dx.doi.org/10.2478/aiht-2020-71-3418.

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AbstractDrug resistance of Pseudomonas aeruginosa is a leading problem in hospital infections. The aim of this study was to determine the best molecular genetic discrimination method for Pseudomonas spp. isolates among 94 outpatients and inpatients and see their grouping by phenotype characteristics (biofilm formation, frequency of serotypes, pigmentation, production of different class of beta-lactamases, and susceptibility to different antibiotic classes) and genotype. The most common serotypes were P1, P6, and P11, while co-productions of pyoverdine and pyocyanin were observed in 70 % of iso
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Barbosa, Thaís Alves, Maria Regina Bentlin, Lígia Maria Suppo de Souza Rugolo, et al. "Molecular Characterization of Gram-Negative Bacilli Isolated from a Neonatal Intensive Care Unit and Phenotypic and Molecular Detection of ESBL and Carbapenemase." Antibiotics 14, no. 4 (2025): 342. https://doi.org/10.3390/antibiotics14040342.

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Introduction: The increase in the rates of multidrug-resistant bacteria in healthcare environments has been recognized as a global public health problem. In view of the scarcity of data on the neonatal population, this study aimed to provide information on the genotypic and epidemiological characteristics of Gram-negative microorganisms isolated from colonization and infection sites in neonates admitted to a tertiary university center of high complexity. Methods: Enterobacterales and non-fermenting Gram-negative bacilli previously collected in a prospective cohort study were submitted to genot
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Jamasbi, Roudabeh J., and Eric M. Proudfoot. "Phenotypic and Genotypic Characteristics of Clinical Isolates of Pseudomonas aeruginosa: Rate of Occurrence and Distribution of Different Serotypes, Antimicrobial Susceptibility Profiles, and Molecular Typing." Laboratory Medicine 39, no. 3 (2008): 155–61. http://dx.doi.org/10.1309/1baww0951n7v71ce.

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Gill, Christian M., Tomefa E. Asempa, and David P. Nicolau. "656. Development and Application of a Pragmatic Algorithm for the Detection of Carbapenemase-Producing Pseudomonas aeruginosa (CP-PA)." Open Forum Infectious Diseases 7, Supplement_1 (2020): S384—S385. http://dx.doi.org/10.1093/ofid/ofaa439.849.

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Abstract Background Historically, carbapenem-resistance in P. aeruginosa (PA) has been mediated by inducible AmpC, drug efflux, and porin loss; however, carbapenemase production is an increasingly recognized entity. Of these mechanisms, carbapenemases can drastically reduce treatment options and rapidly disseminate. Since broad applications of phenotypic (mCIM/eCIM) and PCR-based detection can be labor intensive and costly, we developed an MIC derived algorithm to streamline use of these definitive carbapenemase detection methodologies. Methods To develop the testing criteria, a challenge set
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DANJUMA, LAWAL, MUSLIM ISMAIL, and NURA MUHAMMAD SANI. "SYNTHESIS, CHARACTERIZATION AND ANTIBACTERIAL ACTIVITY OF SILVER NANOPARTICLES SYNTHESIZED FROM Ocimum gratissimum LEAF AGAINST SOME ANTIBIOTIC-RESISTANT CLINICAL ISOLATES." AFRICAN JOURNAL OF PHARMACEUTICAL RESEARCH AND DEVELOPMENT 16, no. 3 (2024): 48–63. https://doi.org/10.59493/ajopred/2024.3.7.

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The rise of antibiotic resistance necessitates exploring alternative antimicrobial agents. Nanomedicine offers promising solutions, by enhancing the antimicrobial potencies of the plant extracts. The present research aimed at synthesizing, characterizing and determining the antibacterial activity of O. gratissimum leaf synthesized Silver Nanoparticles (AgNPs) against some antibiotic-resistant clinical isolates. The test bacteria were collected from Dutse General Hospital and Rasheed Shekoni Teaching Hospital and confirmed using standard phenotypic and genotypic techniques. The AgNPs were prepa
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Hameed, Asif, Mariyam Shahina, Shih-Yao Lin, You-Cheng Liu, and Chiu-Chung Young. "Pseudomonas hussainii sp. nov., isolated from droppings of a seashore bird, and emended descriptions of Pseudomonas pohangensis, Pseudomonas benzenivorans and Pseudomonas segetis." International Journal of Systematic and Evolutionary Microbiology 64, Pt_7 (2014): 2330–37. http://dx.doi.org/10.1099/ijs.0.060319-0.

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Two Gram-staining-negative, aerobic, rod-shaped, non-spore-forming bacterial strains that are motile by a monopolar flagellum, designated CC-AMH-11T and CC-AMHZ-5, were isolated from droppings of a seashore bird off the coast of Hualien, Taiwan. The strains showed 99.7 % mutual pairwise 16S rRNA gene sequence similarity, while exhibiting <96.2 % sequence similarity to strains of other species of the genus Pseudomonas (95.7–95.9 % similarity with type species, Pseudomonas aeruginosa LMG 1242T), and formed a distinct co-phyletic lineage in the phylogenetic trees. The common major fatty acids
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Bahniuk, N., M. Faustova, K. Riesbeck, et al. "THE CORRESPONDENCE OF THE CARBAPENEMASE GENOTYPE AND PHENOTYPIC ANTIMICROBIAL PROFILES OF PSEUDOMONAS AERUGINOSA." Medical and Ecological Problems 27, no. 5-6 (2023): 45–50. http://dx.doi.org/10.31718/mep.2023.27.5-6.06.

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The aim of the study was to determine the correspondence between the carbapenemase genotype and the phenotypic antimicrobial profiles of P. aeruginosa. Materials and methods. The study included 51 clinical isolates of P. aeruginosa, isolated from the patients with post-operative complications of the respiratory organs. The final identification of the obtained isolates was performed in the Riesbeck laboratory using MALDI-ToF (Bruker), followed by the determination of their sensitivity to antimicrobial drugs at the EUCAST Development Laboratory (Växjö, Sweden). Determination of the resistance ge
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Meyer, P., and A. C. Petersson. "130 Characteristics of a new transmissible multiresistant Pseudomonas aeruginosa strain with a unique genotype." Journal of Cystic Fibrosis 5 (2006): S29. http://dx.doi.org/10.1016/s1569-1993(06)80114-1.

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Dave, Alpana, Apurwa Samarth, Roshni Karolia, et al. "Characterization of Ocular Clinical Isolates of Pseudomonas aeruginosa from Non-Contact Lens Related Keratitis Patients from South India." Microorganisms 8, no. 2 (2020): 260. http://dx.doi.org/10.3390/microorganisms8020260.

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P. aeruginosa is the most common Gram-negative organism causing bacterial keratitis. Pseudomonas utilizes various virulence mechanisms to adhere and colonize in the host tissue. In the present study, we examined virulence factors associated with thirty-four clinical P. aeruginosa isolates collected from keratitis patients seeking care at L V Prasad Eye Institute, Hyderabad. The virulence-associated genes in all the isolates were genotyped and characteristics such as antibiotic susceptibility, biofilm formation, swarming motility, pyoverdine production and cell cytotoxicity were analyzed. All t
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Garza-González, Elvira, Paola Bocanegra-Ibarias, Miriam Bobadilla-del-Valle, et al. "Drug resistance phenotypes and genotypes in Mexico in representative gram-negative species: Results from the infivar network." PLOS ONE 16, no. 3 (2021): e0248614. http://dx.doi.org/10.1371/journal.pone.0248614.

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Aim This report presents phenotypic and genetic data on the prevalence and characteristics of extended-spectrum β-lactamases (ESBLs) and representative carbapenemases-producing Gram-negative species in Mexico. Material and methods A total of 52 centers participated, 43 hospital-based laboratories and 9 external laboratories. The distribution of antimicrobial resistance data for Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae complex, Acinetobacter baumannii complex, and Pseudomonas aeruginosa in selected clinical specimens from January 1 to March 31, 2020 was analyzed using the W
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Girolamini, Luna, Ada Dormi, Tiziana Pellati, et al. "Advances in Legionella Control by a New Formulation of Hydrogen Peroxide and Silver Salts in a Hospital Hot Water Network." Pathogens 8, no. 4 (2019): 209. http://dx.doi.org/10.3390/pathogens8040209.

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Legionella surveillance is an important issue in public health, linked to the severity of disease and the difficulty associated with eradicating this bacterium from the water environment. Different treatments are suggested to reduce Legionella risk, however long-term studies of their efficiency are lacking. This study focused on the activity of a new formulation of hydrogen peroxide and silver salts, WTP828, in the hospital hot water network (HWN) to contain Legionella contamination during two years of treatment. The effectiveness of WTP828 was tested measuring physical-chemical and microbiolo
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Urbanowicz, P., R. Izdebski, A. Baraniak, D. Żabicka, W. Hryniewicz та M. Gniadkowski. "Molecular and genomic epidemiology of VIM/IMP-like metallo-β-lactamase-producing Pseudomonas aeruginosa genotypes in Poland". Journal of Antimicrobial Chemotherapy 76, № 9 (2021): 2273–84. http://dx.doi.org/10.1093/jac/dkab188.

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Abstract Objectives To identify key factors of the expansion of metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa (MPPA) in Poland, focusing on the role of clonal epidemic(s). Methods MPPA isolates were typed by PFGE, followed by MLST. blaVIM/IMP MBL genes were amplified and sequenced within class 1 integrons. Their location was assessed by S1 nuclease-hybridization assays. Short-read WGS was performed, and genomes were subjected to SNP-based phylogenetic and resistome analyses. Results Of 1314 MPPA isolates collected in 2005–15 from 212 hospitals, 454 representatives were selected. T
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Köse Çetinkaya, Aslıhan, Deniz Doğru, Güzin Cinel, et al. "Clinical and laboratory findings of patients with cystic fibrosis: a single center experience from Türkiye." Turkish Journal of Pediatric Disease 19, no. 3 (2025): 92–98. https://doi.org/10.12956/tchd.1562280.

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Objective: The objective of this study was to evaluate the clinical characteristics of patients with cystic fibrosis (CF) and to determine whether there is a relationship between nutritional status and pulmonary functions. Additionally, the study aimed to investigate the relationship between the CFTR genotype and the occurrence of cystic fibrosis-related diabetes (CFRD), as well as the impact of CFTR mutations on the severity of CF pulmonary disease. Material and Methods: The data of 300 CF patients were retrospectively analyzed. Clinical and laboratory characteristics were obtained from unit
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Heiba, Samy A. A., Ibthal S. El-Demerdash, and Shimaa E. Rashad. "Evaluation of Biological Control of Sorghum Strains Using Bacillus Thuringiensis and Pseudomonas Aeruginosa Under Drought Stress." Journal of Advanced Zoology 44, no. 4 (2023): 8–22. http://dx.doi.org/10.17762/jaz.v44i4.1321.

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Background: Sorghum is an economically significant staple food crop for more than half a billion people in developing nations, especially in arid and semi-arid locations where drought stress is a significant limiting factor. Despite usually being regarded as tolerant, sorghum suffers severely from drought stress, which lowers its productivity and nutritional quality throughout its principal cultivation areas. Objective: Improvements in DNA fingerprinting by ISSRs, SSRs, and RAPD markers have also been employed in sorghum genetic modification (GMOs) to enhance the economic characteristics of th
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Shen, Lijuan, Manqian Huang, and Nanli Xie. "Experimental Study on Streptococcus agalactiae Genotype and Erythromycin Resistance in Neonatal Sepsis." Cellular and Molecular Biology 67, no. 6 (2022): 100–106. http://dx.doi.org/10.14715/cmb/2021.67.6.14.

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This study aimed to evaluate Streptococcus agalactiae genotype and erythromycin resistance in neonatal sepsis. After obtaining the mothers’ informed consent, trained nurses sampled 430 neonatal specimens of sepsis from the ear canal, oral cavity and umbilical cord immediately after childbirth and implemented a cross-sectional study. By Gram staining, morphology, hemolysis mode, catalase and CAMP tests, the isolate was identified as S. agalactiae. All 455 isolates were tested for antimicrobial susceptibility by the disc diffusion method. Multilocus sequence typing was used to serotype S. agalac
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Hamasaeed, Payman Akram. "Resistance to bacterial infection, complication occurring after cardiac surgery." Cellular and Molecular Biology 66, no. 8 (2020): 20–25. http://dx.doi.org/10.14715/cmb/2020.66.8.4.

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To analyze the occurrence of resistant bacterial infection in patients undergoing cardiac surgery hospitalized in the surgical specialty hospital, in Erbil city, Iraq. A prospective study was done on a total of 138 patients operated and hospitalized in an intensive care unit and surgical wards. Bacterial isolates identification was done according to cultural characteristics, microscopic examination, some biochemical tests, analytic Profile Index 20E& API Staph, confirmed with VITEK® 2 compact system (BioMérieux). Antimicrobial susceptibility for disc diffusion tested to 17 antimicrobial ag
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Iwańska, Agnieszka, Elżbieta Anna Trafny, Michał Czopowicz, and Ewa Augustynowicz-Kopeć. "Phenotypic and genotypic characteristics of Pseudomonas aeruginosa isolated from cystic fibrosis patients with chronic infections." Scientific Reports 13, no. 1 (2023). http://dx.doi.org/10.1038/s41598-023-39005-9.

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AbstractPatients with cystic fibrosis are predisposed to chronic respiratory tract infections caused by Pseudomonas aeruginosa. As the disease progresses, the microorganism diversifies into genotypically and phenotypically different strains which may coexist in the patient's airways for years. Adaptation of the microorganism to the airways of patients with cystic fibrosis probably occurs in response to the host's airway environment, the elements of the immune system and antibiotic therapy. Due to the chronic persistence of the microorganism in the airways, a comprehensive molecular analysis wa
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Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, and Snehasis Jana. "Antibiogram, Biochemical Reactions, and Genotypic Pattern of Biofield Treated Pseudomonas aeruginosa." Journal of Tropical Diseases 4, no. 1 (2015). https://doi.org/10.5281/zenodo.166324.

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<strong>Introduction:</strong> Complementary and alternative medicine such as biofield energy therapies are highly popular in biomedical health care. The study evaluates the impact of Mr. Trivedi&rsquo;s biofield energy treatment on Pseudomonas aeruginosa (P. aeruginosa) to evaluate its phenotypic and genotypic characteristics. <strong>Methods:</strong> P. aeruginosa ATCC 10145 (American Type Culture Collection) was procured from Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to biofield treatment and analyzed for antibiogram, biochemic
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Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, and Snehasis Jana. "Antibiogram, Biochemical Reactions, and Genotypic Pattern of Biofield Treated Pseudomonas aeruginosa." Journal of Tropical Diseases 4, no. 1 (2015). https://doi.org/10.5281/zenodo.193946.

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Introduction: Complementary and alternative medicine such as biofield energy therapies are highly popular in biomedical health care. The study evaluates the impact of Mr. Trivedi&rsquo;s biofield energy treatment on Pseudomonas aeruginosa (P. aeruginosa) to evaluate its phenotypic and genotypic characteristics. Methods: P. aeruginosa ATCC 10145 (American Type Culture Collection) was procured from Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to biofield treatment and analyzed for antibiogram, biochemical reactions, and biotype number u
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Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, and Gopal Nayak. "Antibiogram, Biochemical Reactions, and Genotypic Pattern of Biofield Treated Pseudomonas aeruginosa." Omics Publishing Group, October 7, 2015. https://doi.org/10.5281/zenodo.813958.

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Introduction: Complementary and alternative medicine such as biofield energy therapies are highly popular in biomedical health care. The study evaluates the impact of Mr. Trivedi's biofield energy treatment on Pseudomonas aeruginosa (P. aeruginosa) to evaluate its phenotypic and genotypic characteristics. Methods: P. aeruginosa ATCC 10145 (American Type Culture Collection) was procured from Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to biofield treatment and analyzed for antibiogram, biochemical reactions, and biotype number using a
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Ebrahim, H., S. Haldenby, M. P. Moore, A. A. Dashti, R. V. Floyd, and J. L. Fothergill. "Genotypic and phenotypic analyses of two distinct sets of Pseudomonas aeruginosa urinary tract isolates." Journal of Medical Microbiology 74, no. 2 (2025). https://doi.org/10.1099/jmm.0.001971.

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Introduction. Urinary tract infections (UTIs) are associated with a high burden of morbidity, mortality and cost. Pseudomonas aeruginosa employs a myriad of virulence factors, including biofilm formation and motility mechanisms, to cause infections including persistent UTIs. P. aeruginosa is highly resistant to antibiotics, and the World Health Organization has identified it as a pathogen for which novel antimicrobials are urgently required. Gap statement. Genotypic and phenotypic characterization of P. aeruginosa from UTIs is underreported. In addition, the rise of antimicrobial resistance (A
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Safarirad, Somayeh, Mohsen Arzanlou, Jafar Mohammadshahi, Hamid Vaez, Amirhossein Sahebkar, and Farzad Khademi. "Prevalence and Characteristics of Metallo-beta-Lactamase-positive and High-risk Clone ST235 Pseudomonas aeruginosa at Ardabil Hospitals." Jundishapur Journal of Microbiology 14, no. 3 (2021). http://dx.doi.org/10.5812/jjm.115819.

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Background: Carbapenems are the most commonly administered drugs for the treatment of multidrug-resistant Pseudomonas aeruginosa (MDR P. aeruginosa) infections. However, carbapenem-resistant P. aeruginosa is spreading rapidly and has led to a new threat to human health worldwide. Objectives: The current study aimed to determine the prevalence of imipenem-resistant P. aeruginosa, detect metallo-β-lactamase (MBL)-producer isolates, and evaluate their clonal relationships in strains isolated from patients referring to the hospitals of Ardabil city, Iran. Methods: The resistance rate to imipenem w
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"Phenotypic and Genotypic Characteristics of Antimicrobial and Disinfectant Resistance of Gram-negative Bacteria Involved in Early Broiler Chick Mortality." International Journal of Veterinary Science 10, no. 2 (2021): 129–34. http://dx.doi.org/10.47278/journal.ijvs/2020.033.

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One of the most challenges facing the poultry industry in Egypt is the early mortality among broiler chicks during the first ten days of the rearing cycle, especially which caused by antimicrobial resistant Gram-negative bacteria. This study was conducted for the determination of the prevalence of Gram-negative bacteria involved in early mortality among broiler chicks. Antibiogram profile and antibiotic and disinfectant resistance genes were also performed. Five hundred samples (liver, yolk sac, cecum, spleen, and heart) from freshly dead affected chicks were cultured on different media for is
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Rengaraj, Ramya, Natarajan Muninathan, Sivaranjini Alagiri та Arumugam Suresh. "A Study of Genotypic Characterization of ESBL and MBL Genes of β-Lactamase Producing Pseudomonas aeruginosa in Various Clinical Samples". Journal of Pure and Applied Microbiology, 2 травня 2024. http://dx.doi.org/10.22207/jpam.18.2.15.

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Pseudomonas aeruginosa, a Gram-negative bacterium, presents a substantial challenge in healthcare due to its adaptability and resistance. This study delves into its genotypic characteristics, focusing on ESBL and MBL genes. The prevalence of P. aeruginosa in nosocomial infections, the research aims to decipher resistance mechanisms crucial for tailored interventions. The study includes 170 non-repetitive clinical samples with protocols. Antibiotic susceptibility testing reveals diverse resistance patterns. Molecular detection of ESBL and MBL genes involves DNA isolation, PCR amplification, and
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Eladawy, Mohamed, Jonathan C. Thomas, and Lesley Hoyles. "Phenotypic and genomic characterization of Pseudomonas aeruginosa isolates recovered from catheter-associated urinary tract infections in an Egyptian hospital." Microbial Genomics 9, no. 10 (2023). http://dx.doi.org/10.1099/mgen.0.001125.

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Catheter-associated urinary tract infections (CAUTIs) represent one of the major healthcare-associated infections, and Pseudomonas aeruginosa is a common Gram-negative bacterium associated with catheter infections in Egyptian clinical settings. The present study describes the phenotypic and genotypic characteristics of 31 P . aeruginosa isolates recovered from CAUTIs in an Egyptian hospital over a 3 month period. Genomes of isolates were of good quality and were confirmed to be P. aeruginosa by comparison to the type strain (average nucleotide identity, phylogenetic analysis). Clonal diversity
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Kamali, Esmat, Ailar Jamali, Abdollah Ardebili, Freshteh Ezadi, and Alireza Mohebbi. "Evaluation of antimicrobial resistance, biofilm forming potential, and the presence of biofilm-related genes among clinical isolates of Pseudomonas aeruginosa." BMC Research Notes 13, no. 1 (2020). http://dx.doi.org/10.1186/s13104-020-4890-z.

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Abstract Objectives Pseudomonas aeruginosa is known as a leading cause of nosocomial infections worldwide. Antimicrobial resistance and biofilm production, as two main virulence factors of P. aeruginosa, are responsible for the persistence of prolonged infections. In this study, antimicrobial susceptibility pattern and phenotypic and genotypic characteristics of biofilm of P. aeruginosa were investigated. Results A total of 80 clinical P. aeruginosa isolates were obtained. Isolates showed resistance to all antibiotics with a rate from 12.5% (n = 10) against amikacin and piperacillin/tazobactam
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Yanning, Ma, Liu Jie, Bao Chunmei, et al. "Phenotypic and Genotypic Characteristics of Pseudomonas aeruginosa Causing Bloodstream Infection from Six Tertiary Hospitals in Beijing, China." Journal of Infectious Diseases and Epidemiology 4, no. 4 (2018). http://dx.doi.org/10.23937/2474-3658/1510061.

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Carneiro, Gabriella Vieira, Fabiana Sodré de Oliveira, Leandro Alves Pereira, Érica Rodrigues Mariano de Almeida Rezende, Luciana Carneiro Pereira Gonçalves, and Vivian Mara Gonçalves de Oliveira Azevedo. "Association between phenotypic and genotypic characteristics and disease severity in individuals with cystic fibrosis." Revista Paulista de Pediatria 41 (2023). http://dx.doi.org/10.1590/1984-0462/2023/41/2021286.

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Abstract Objective: To analyze the association between phenotypic and genotypic characteristics and disease severity in individuals with cystic fibrosis treated at a reference center in Minas Gerais, Brazil. Methods: This is a retrospective study that collected clinical and laboratory data, respiratory and gastrointestinal manifestations, type of treatment, Shwachman-Kulczycki score, and mutations from the patients’ medical records. Results: The sample included 50 participants aged one to 33 years, 50% of whom were female. Out of the one hundred alleles of the Cystic Fibrosis Transmembrane Con
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Mossop, Micaela, Luca Robinson, Jhih-Hang Jiang, et al. "Characterisation of key genotypic and phenotypic traits of clinical cystic fibrosis Staphylococcus aureus isolates." Journal of Medical Microbiology 72, no. 6 (2023). http://dx.doi.org/10.1099/jmm.0.001703.

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Introduction. One third of people with CF in the UK are co-infected by both Staphylococcus aureus and Pseudomonas aeruginosa . Chronic bacterial infection in CF contributes to the gradual destruction of lung tissue, and eventually respiratory failure in this group. Gap Statement. The contribution of S. aureus to cystic fibrosis (CF) lung decline in the presence or absence of P. aeruginosa is unclear. Defining the molecular and phenotypic characteristics of a range of S. aureus clinical isolates will help further understand its pathogenic capabilities. Aim. Our objective was to use molecular an
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Aminatun, Tien, Anna Rakhmawati, Sri Atun, Arifudin Idrus, Doly Risdo Simbolon, and Laode Restele. "Characteristics of rhizobacteria in potential hyperaccumulator vegetation and their resistance to gold mine tailing stress." Journal of Water and Land Development, January 9, 2024, 209–18. http://dx.doi.org/10.24425/jwld.2024.149122.

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The use of local bacteria is preferred in bioleaching as an environmental-friendly alternative technology in gold mining. In a preliminary study, rhizobacteria were isolated and cultured from three types of hyperaccumulator vegetation from the Ratatotok gold mine, Indonesia, namely Pteris vittata L., Syzygium aromaticum L., and Swietenia mahagoni Jacq. These rhizobacteria still need to be characterised and identified. This study is aimed to cover bacterial phenotypic characterisation, assessment of bacteria resistance to tailing, and identification of bacterial strains the exhibit the highest
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Hemmati, Jaber, Mohsen Nazari, Amjad Ahmadi, et al. "In vitro evaluation of biofilm phenotypic and genotypic characteristics among clinical isolates of Pseudomonas aeruginosa in Hamadan, West of Iran." Journal of Applied Genetics, November 28, 2023. http://dx.doi.org/10.1007/s13353-023-00811-6.

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Rudra, Bashudev, Louise Duncan, Ajit J. Shah, Haroun N. Shah, and Radhey S. Gupta. "Phylogenomic and comparative genomic studies robustly demarcate two distinct clades of Pseudomonas aeruginosa strains: proposal to transfer the strains from an outlier clade to a novel species Pseudomonas paraeruginosa sp. nov." International Journal of Systematic and Evolutionary Microbiology 72, no. 11 (2022). http://dx.doi.org/10.1099/ijsem.0.005542.

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The strains of Pseudomonas aeruginosa exhibit considerable differences in their genotypic and pathogenic properties. To clarify their evolutionary/taxonomic relationships, comprehensive phylogenomic and comparative genomic studies were conducted on the genome sequences of 212 P . aeruginosa strains covering their genetic diversity. In a phylogenomic tree based on 118 conserved proteins, the analysed strains formed two distinct clades. One of these clades, Clade-1, encompassing &gt;70 % of the strains including the type strain DSM 50071T, represents the species P. aeruginosa sensu stricto. Clad
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Sun, Zhewei, Feifei Yang, Jian Ji, et al. "Dissecting the genotypic features of a fluoroquinolone-resistant Pseudomonas aeruginosa ST316 sublineage causing ear infections in Shanghai, China." Microbial Genomics 9, no. 4 (2023). http://dx.doi.org/10.1099/mgen.0.000989.

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Limited information is available regarding the genomic characteristics of P. aeruginosa causing ear infections. Our aim is to characterize the genotypic features of an emerging ST316 sublineage causing aural infections in Shanghai. A total of 199 ear swab isolates were subjected to whole genome sequencing (WGS). Complete genomes for two isolates were resolved. We showed this recently emerged sublineage exhibited high-level resistance to fluoroquinolones (FQs) primarily by accumulation of known mutations in quinolone resistance determining regions (QRDRs). Loss-of-function mutations in mexR and
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Kim, Minkyung, Byoung-Hee Lee, Ki-Eun Lee, and Woojun Park. "Flavobacterium phycosphaerae sp. nov. isolated from the phycosphere of Microcystis aeruginosa." International Journal of Systematic and Evolutionary Microbiology 71, no. 3 (2019). http://dx.doi.org/10.1099/ijsem.0.004735.

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A novel aerobic bacterial strain, designated MK012T, was isolated from the phycosphere of Microcystis aeruginosa . MK012T appears to be Gram-stain-negative, non-motile and rod-shaped and has yellow pigments. Phylogenetic analysis using 16S rRNA revealed that this bacterium was most closely related to Flavobacterium buctense T7T (=JCM 30750T; 97.5 %), Flavobacterium dankookense ARSA-19T (=KCTC 23179T; 97.5 %) and Flavobacterium macrobrachii an-8T (=DSM 22219T; 97.3 %). The genome size and genomic DNA G+C content of MK012T were estimated at 3.3 Mbp and 37.6 mol%, respectively. The average nucleo
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Saleh, Rasha Hadi, Habeeb S. Naher, and Mohammed AK Al-saadi. "Molecular Investigation of hemolytic phospholipase C Encoding Genes in Clinical Isolates of Pseudomonas aeruginosa." International Journal of Research in Pharmaceutical Sciences 9, SPL1 (2018). http://dx.doi.org/10.26452/ijrps.v9ispl1.1387.

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This study is aimed to isolate P.aeruginosa from different clinical cases and to detect the prevalence of virulence genes encoding hemolytic phospholipase C(plcH)in these clinical isolates. In this study a total of 422 clinical samples including burn,wound,ear,urine,abscess and stool were aseptically taken from out- and inpatients who admitted into two hospitals in Hilla City (Teaching Al-Hilla Hospital and Babylon Hospital for Maternity and children during a period of three months. All samples were subjected to bacterial cultivation for the isolation of P.aeruginosa. The isolated P.aeruginosa
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Mizuno, Gustavo, Thales Polis, Jacqueline Ferrari, et al. "148. Imipenem-Relebactam activity and genotypic characteristics of Carbapenem-resistant Enterobacterales and Pseudomonas aeruginosa Isolates from Latin American Infections - Study for Monitoring Antimicrobial Resistance Trends (SMART) 2017 – 2020." Open Forum Infectious Diseases 9, Supplement_2 (2022). http://dx.doi.org/10.1093/ofid/ofac492.226.

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Abstract Background Imipenem/relebactam (IMI/REL) is a combination of Imipenem with Relebactam, an inhibitor of class A and C β-lactamases and has been approved in the US and EU, but not in Latin America. This report evaluates the in vitro activity of IMI/REL and comparators against Latin America (LATAM) Enterobacterales and P. aeruginosa (PSA) and the frequency of carbapenemase encoding genes (CEG) among gram-negative bacilli (GNB) isolated through the SMART Program (2017-2020). Methods 21606 nonconsecutive GNB isolates were collected in 10 LATAM countries. MICs for amikacin (AK), ceftazidime
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Jenkins, Stephen, Nathan A. Ledeboer, Lars F. Westblade, et al. "Evaluation of NG-Test Carba 5 for Rapid Phenotypic Detection and Differentiation of Five Common Carbapenemase Families: Results of a Multicenter Clinical Evaluation." Journal of Clinical Microbiology 58, no. 7 (2020). http://dx.doi.org/10.1128/jcm.00344-20.

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ABSTRACT NG-Test Carba 5 is a rapid in vitro multiplex immunoassay for the phenotypic detection and differentiation of five common carbapenemase families (KPC, OXA-48-like, VIM, IMP, and NDM) directly from bacterial colonies. The assay is simple to perform and has recently received U.S. Food and Drug Administration clearance. A method comparison study was performed at geographically diverse medical centers (n = 3) in the United States, where 309 Enterobacterales and Pseudomonas aeruginosa isolates were evaluated by NG-Test Carba 5 (NG Biotech, Guipry, France), the Xpert Carba-R assay (Cepheid,
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AL-Mashhadani, Riyam Khalid Attallah, Mohammed Fadhil AboKsour, and Osama Abdul Azeez Dakhil. "Determine Biofilm Genes in Pseudomonas Aeruginosa Isolated from Clinical and Environmental Samples." Journal of Contemporary Medical Sciences 10, no. 3 (2024). http://dx.doi.org/10.22317/jcms.v10i3.1557.

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Objective: Isolates of Pseudomonas aeruginosa are an extremely adaptable bacterium that causes opportunistic diseases because of its varied metabolic pathways, genes, virulence factors, and considerable antibiotic resistance. Methods: A total of 293 samples were collected from different places: 193 samples (% 65.87) of human samples and 100 samples (% 34.13) of wastewater samples in the period between 3rd September to 15th November 2023). Bacterial isolates were identified according to microscopic, cultural, and genetic characteristics. Antibiotic susceptibility of bacterial isolates was deter
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