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Artykuły w czasopismach na temat „Aeruginosa Phenotypic Characteristics”

Autor: Grafiati

Data publikacji: 3 czerwca 2025

Data aktualizacji: 31 lipca 2025

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1

Depke, Tobias, Janne Gesine Thöming, Adrian Kordes, Susanne Häussler, and Mark Brönstrup. "Untargeted LC-MS Metabolomics Differentiates Between Virulent and Avirulent Clinical Strains of Pseudomonas aeruginosa." Biomolecules 10, no. 7 (2020): 1041. http://dx.doi.org/10.3390/biom10071041.

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Pseudomonas aeruginosa is a facultative pathogen that can cause, inter alia, acute or chronic pneumonia in predisposed individuals. The gram-negative bacterium displays considerable genomic and phenotypic diversity that is also shaped by small molecule secondary metabolites. The discrimination of virulence phenotypes is highly relevant to the diagnosis and prognosis of P. aeruginosa infections. In order to discover small molecule metabolites that distinguish different virulence phenotypes of P. aeruginosa, 35 clinical strains were cultivated under standard conditions, characterized in terms of

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M. Ismail, Yasser, Sahar M. Fayed, Fatma M. Elesawy, Nora Z Abd El-Halim, and Ola S. El-Shimi. "Phenotypic and Molecular Characteristics of Pseudomonas Aeruginosa Isolated from Burn Unit." EJMM-Volume 30-Issue 1 30, no. 1 (2021): 19–28. http://dx.doi.org/10.51429/ejmm30103.

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Background: The biggest concern for a burn team is a nosocomial infection in burn patients, which is a significant health issue. Pseudomonas aeruginosa is an extremely troublesome drug-resistant bacterium in the world today. We are now faced with rising P. aeruginosa pan-drug-resistant clones in hospital settings. Objectives: To evaluate the distribution of different virulence factors generated by P. aeruginosa isolated from burn wound infections, together with its antimicrobial susceptibility. Methodology: The isolates reported as P. aeruginosa were further tested for the presence of various

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Bahniuk, N., M. Faustova, K. Riesbeck, et al. "THE CORRESPONDENCE OF THE CARBAPENEMASE GENOTYPE AND PHENOTYPIC ANTIMICROBIAL PROFILES OF PSEUDOMONAS AERUGINOSA." Medical and Ecological Problems 27, no. 5-6 (2023): 45–50. http://dx.doi.org/10.31718/mep.2023.27.5-6.06.

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The aim of the study was to determine the correspondence between the carbapenemase genotype and the phenotypic antimicrobial profiles of P. aeruginosa. Materials and methods. The study included 51 clinical isolates of P. aeruginosa, isolated from the patients with post-operative complications of the respiratory organs. The final identification of the obtained isolates was performed in the Riesbeck laboratory using MALDI-ToF (Bruker), followed by the determination of their sensitivity to antimicrobial drugs at the EUCAST Development Laboratory (Växjö, Sweden). Determination of the resistance ge

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Sieswerda, Elske, Thijs Bosch, Jacqueline M. Lankelma, Leo M. Schouls, and Karin van Dijk. "Vitek® 2 MICs as first-line phenotypic screening method for carbapenemase-producing Pseudomonas aeruginosa." Future Microbiology 16, no. 11 (2021): 777–81. http://dx.doi.org/10.2217/fmb-2020-0024.

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Aim: To define sensitivity and specificity of Vitek® 2 MICs as phenotypic screening method for carbapenemase-producing Pseudomonas aeruginosa. Materials & methods: We determined Vitek® 2 MICs of antipseudomonal antimicrobials in 130 unrelated carbapenemase-producing P. aeruginosa and 129 carbapenemase-negative P. aeruginosa isolates within a Dutch carbapenemase-surveillance database. We calculated test characteristics of single and combined antimicrobial MICs for carbapenemase production. Results: Vitek® 2 MIC above epidemiological cutoff of both imipenem and tobramycin or ciprofloxacin an

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5

Eyo, A.-A., D. Udoh, N. Umoh, O. Ogba, E. Ibeneme, and A. Asuquo. "Carbapenemase-Producing Pseudomonas aeruginosa Isolates: Prevalence and Their Phenotypic Characteristics in Four Health Facilities in Calabar, Nigeria." Acta Microbiologica Bulgarica 40, no. 4 (2024): 457–67. https://doi.org/10.59393/amb24400405.

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Emergence of multi-drug-resistant Pseudomonas aeruginosa is one of the challenging problems of antimicrobial chemotherapy which has become a global public health concern. This cross-sectional study was designed to determine the antibiotic resistance profile of this important pathogen and the prevalence of carbapenemase-producing P. aeruginosa strains isolated in four hospitals in Calabar Metropolis. Three hundred and twelve suspected P. aeruginosa strains non-repetitively isolated from different clinical speci¬mens in four hospitals were characterized and re-identified with the Microgen™ GNA+B

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6

Chance, Deborah L., Wei Wang, James K. Waters, and Thomas P. Mawhinney. "Insights on Pseudomonas aeruginosa Carbohydrate Binding from Profiles of Cystic Fibrosis Isolates Using Multivalent Fluorescent Glycopolymers Bearing Pendant Monosaccharides." Microorganisms 12, no. 4 (2024): 801. http://dx.doi.org/10.3390/microorganisms12040801.

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Pseudomonas aeruginosa contributes to frequent, persistent, and, often, polymicrobial respiratory tract infections for individuals with cystic fibrosis (CF). Chronic CF infections lead to bronchiectasis and a shortened lifespan. P. aeruginosa expresses numerous adhesins, including lectins known to bind the epithelial cell and mucin glycoconjugates. Blocking carbohydrate-mediated host–pathogen and intra-biofilm interactions critical to the initiation and perpetuation of colonization offer promise as anti-infective treatment strategies. To inform anti-adhesion therapies, we profiled the monosacc

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7

Gajdács, Márió, Edit Urbán, Péter Pallós, et al. "Phenotypic characteristics of environmental Pseudomonas aeruginosa: an in vitro study on epidemiological aspects." Acta Biologica Szegediensis 67, no. 1 (2023): 35–44. http://dx.doi.org/10.14232/abs.2023.1.35-44.

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Pseudomonas aeruginosa is one of the most commonly isolated species among non-fermenting Gram-negative bacteria, both from clinical samples and from environmental sources. The survival of the species in harsh conditions is ensured by the production of a protective biofilm; assessment of biofilm-forming capacity aids future pathogen eradication strategies. The aim of our present study was to assess the relationship between antibiotic resistance, biofilm-forming capacity and other phenotypic virulence factors in environmental P. aeruginosa isolates. One hundred and fourteen (n = 114) isolates we

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Ghorbani, Ghasem, Ebrahim Rahimi, and Amir Shakerian. "Antibiotic resistance’s Genotypic and Phenotypic Characteristics and the Frequency of Virulence Factors in P. aeruginosa Isolates Isolated from Water Samples in Iran." BioMed Research International 2022 (September 30, 2022): 1–7. http://dx.doi.org/10.1155/2022/7076433.

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Pseudomonas aeruginosa is a pathogenic bacterium that can contaminate water. In this study, 430 water samples were evaluated for P. aeruginosa, antibiotic resistance, and the abundance of virulence factors. P. aeruginosa was isolated from 28 (6.51%) water samples. Among the types of water, well and spring water showed the highest P. aeruginosa with, respectively, 20 (15.6%) and 5 (8.06%) positive samples per type of samples. Drinking water and mineral water showed minor contamination with P. aeruginosa. The prevalence of antibiotic resistance against meropenem, imipenem, erythromycin, gentamic

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Winstanley, Craig, Stephen B. Kaye, Timothy J. Neal, Helen J. Chilton, Silvia Miksch, and C. Anthony Hart. "Genotypic and phenotypic characteristics of Pseudomonas aeruginosa isolates associated with ulcerative keratitis." Journal of Medical Microbiology 54, no. 6 (2005): 519–26. http://dx.doi.org/10.1099/jmm.0.46005-0.

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Nagant, C., M. Seil, A. Nachtergael, S. Dulanto, and J. P. Dehaye. "Contribution of the production of quormones to some phenotypic characteristics of Pseudomonas aeruginosa clinical strains." Journal of Medical Microbiology 62, no. 7 (2013): 951–58. http://dx.doi.org/10.1099/jmm.0.050807-0.

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The contribution of quorum sensing in some phenotypic and pathogenic characteristics of Pseudomonas aeruginosa was studied. The production of acylhomoserine lactones (AHL) by planktonic cultures of eight clinical and reference strains of P. aeruginosa was evaluated using two biosensors. The adhesion of the bacteria on a surface (Biofilm Ring Test ®, BFRT), their capacity to develop a biofilm (crystal violet staining method, CVSM), their sensitivity to tobramycin and their secretion of proteases or of rhamnolipids were also measured. The production and the release of AHL widely varied among the

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11

Ullah, Waheed, Muhammad Qasim, Hazir Rahman, Yan Jie, and Noor Muhammad. "Beta-lactamase-producing Pseudomonas aeruginosa : Phenotypic characteristics and molecular identification of virulence genes." Journal of the Chinese Medical Association 80, no. 3 (2017): 173–77. http://dx.doi.org/10.1016/j.jcma.2016.08.011.

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Winstanley, C., J. L. Fothergill, S. Panagea, C. H. M. Smart, M. J. Walshaw, and C. A. Hart. "71 Phenotypic and genotypic characteristics of a Cystic Fibrosis epidemic strain of Pseudomonas aeruginosa." Journal of Cystic Fibrosis 5 (2006): S16. http://dx.doi.org/10.1016/s1569-1993(06)80062-7.

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13

Smania, Andrea M., Ignacio Segura, Roberto J. Pezza, Cecilia Becerra, Inés Albesa, and Carlos E. Argaraña. "Emergence of phenotypic variants upon mismatch repair disruption in Pseudomonas aeruginosa." Microbiology 150, no. 5 (2004): 1327–38. http://dx.doi.org/10.1099/mic.0.26751-0.

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MutS is part of the bacterial mismatch repair system that corrects point mutations and small insertions/deletions that fail to be proof-read by DNA polymerase activity. In this work it is shown that the disruption of the P. aeruginosa mutS gene generates the emergence of diverse colony morphologies in contrast with its parental wild-type strain that displayed monomorphic colonies. Interestingly, two of the mutS morphotypes emerged at a high frequency and in a reproducible way and were selected for subsequent characterization. One of them displayed a nearly wild-type morphology while the other

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14

Tomaś, Natalia, Kamila Myszka, and Łukasz Wolko. "Potassium Chloride, Sodium Lactate and Sodium Citrate Impaired the Antimicrobial Resistance and Virulence of Pseudomonas aeruginosa NT06 Isolated from Fish." Molecules 28, no. 18 (2023): 6654. http://dx.doi.org/10.3390/molecules28186654.

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Sodium chloride (NaCl) is a commonly used additive in minimally processed fish-based products. The addition of NaCl to fish products and packaging in a modified atmosphere is usually efficient with regard to limiting the occurrence of the aquatic environmental pathogen Pseudomonas aeruginosa. Given the negative effects of excess NaCl in the diet, there is a growing demand to reduce NaCl in food products with safer substituents, but the knowledge of their impact on antibiotic resistant P. aeruginosa is limited. This study aimed to evaluate the physiological and transcriptome characteristics of

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15

de Souza, Paula Araujo, Milena Cristina Silva dos Santos, Rebeca Vitória da Silva Lage de Miranda, et al. "Evaluation of Antimicrobial Resistance Patterns of Pseudomonas aeruginosa Strains Isolated among COVID-19 Patients in Brazil Typed by Fourier-Transform Infrared Spectroscopy." Life 14, no. 9 (2024): 1079. http://dx.doi.org/10.3390/life14091079.

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This study aimed to characterize Pseudomonas aeruginosa strains isolated from hospitalized patients during the COVID-19 pandemic. This was achieved using phenotypic and molecular techniques, including their antimicrobial resistance profile and biofilm formation. Eighteen strains were isolated from a hospital in Rio de Janeiro, Brazil, and identified by VITEK®2, MALDI-TOF/MS (VITEK MS® and MALDI Biotyper®), and 16S rRNA sequencing. Fourier-transform infrared (FTIR) spectroscopy, antimicrobial susceptibility testing, and biofilm formation and disinfectant tolerance tests were applied to evaluate

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16

Khan, Karim, Zeba Parveen Imran, FARYAL ANJUM, Mariya Yaqoob, Uzma Munir, and Urooj Haroon. "Carbapenem Resistant Pseudomonas aeruginosa, Enterobacteriaceae, and Acinetobacter baumannii , Prevalence, Biochemical Identification and Clinical Characteristics in Karachi, Pakistan." Journal of Sustainable Environment 2, no. 2 (2023): 21–27. http://dx.doi.org/10.58921/jse.02.02.056.

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Antibiotic resistant pathogens are affecting the community and healthcare institutions all over the world. Pakistan is a developing country and resistance to drugs is the main issue and is of great importance. Current study is focused on isolation and identification of bacterial pathogens, i.e. member of Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter baumannii for the evaluation of prevalence, distribution of sensitivity and antibiogram of different antibiotics and carbapenem resistance isolates with phenotypic detection of resistant gene. Total 200 samples of different sources w

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Fontana, Lauren, Morgan Hakki, Richard Zhang, et al. "803. The Impact of Bundled Interventions to Decrease Transmission of Drug-Resistant Pseudomonas aeruginosa from Wastewater Drain Sites on a Hematologic Malignancy/Hematopoietic Stem Cell Transplant Unit." Open Forum Infectious Diseases 8, Supplement_1 (2021): S496. http://dx.doi.org/10.1093/ofid/ofab466.999.

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Abstract Background Wastewater drain (WWD) sites are an important reservoir for amplification, propagation and transmission of multidrug resistant organisms. We observed an increase in the incidence of carbapenem and fluoroquinolone non-susceptible (CP-NS and FQ-NS) P. aeruginosa bloodstream infections (BSI) among patients on our hematologic malignancies (HM) and hematopoietic cell transplant (HCT) unit. The incidence of CP-NS/FQ-NS P. aeruginosa BSI from 2012 through May 2021 is represented in Figure 1. We sought to determine the impact of low-cost, low-barrier interventions targeting WWD sit

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Koutsogiannou, M., E. Drougka, A. Liakopoulos, et al. "Comparative epidemiological study of Pseudomonas aeruginosa isolates during two time periods in a tertiary care hospital." ACTA MICROBIOLOGICA HELLENICA 59, no. 4 (2014): 43–56. https://doi.org/10.5281/zenodo.10017365.

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Pseudomonas aeruginosa is a nosocomial pathogen of clinical importance carrying virulence and antibiotic resistance determinants. In the present study, phenotypic and genotypic characteristics of P. aeruginosa isolates collected at the University General Hospital of Patras (UGHP) in two time periods were investigated.Two groups of P. aeruginosa clinical isolates were compared collected with one year interval, in the UGHP. Group A consisted of 120 isolates recovered during one year (2004), whereas, group B included 240 recovered during a two year period (2006-2007). P. aeruginosa isolates were

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Milojković, Marko, Željka Nenadović, Slaviša Stanković, et al. "Phenotypic and genetic properties of susceptible and multidrug-resistant Pseudomonas aeruginosa isolates in Southern Serbia." Archives of Industrial Hygiene and Toxicology 71, no. 3 (2020): 231–50. http://dx.doi.org/10.2478/aiht-2020-71-3418.

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AbstractDrug resistance of Pseudomonas aeruginosa is a leading problem in hospital infections. The aim of this study was to determine the best molecular genetic discrimination method for Pseudomonas spp. isolates among 94 outpatients and inpatients and see their grouping by phenotype characteristics (biofilm formation, frequency of serotypes, pigmentation, production of different class of beta-lactamases, and susceptibility to different antibiotic classes) and genotype. The most common serotypes were P1, P6, and P11, while co-productions of pyoverdine and pyocyanin were observed in 70 % of iso

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Zulfirya Z., Sadeeva, I. E. Novikova, R. A. Schakirzyanova, et al. "Genetic characteristics of antimicrobial resistance mechanisms in Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter baumannii isolated from blood and cerebrospinal fluid of children." Clinical Microbiology and Antimicrobial Chemotherapy 23, no. 4 (2021): 388–99. http://dx.doi.org/10.36488/cmac.2021.4.388-399.

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Objective. To assess antimicrobial susceptibility, presence of resistance genes and determine the phenotypic groups of K. pneumoniae, P. aeruginosa and A. baumannii isolated from blood and cerebrospinal fluid of children with nosocomial infections in intensive care units from 2014 to 2020. Materials and Methods. Minimum inhibitory concentrations of antibiotics were determined using the serial broth microdilution method. The identification of genes encoding the production of carbapenemases was carried out using hybridization fluorescence detection. Results. A total of 63 isolates of K. рneumoni

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Bocharova, Yu A., K. V. Kuleshov, Igor V. Chebotar, and N. A. Mayanskiy. "The phenomenon of changing aztreonam susceptibility in Pseudomonas aeruginosa during formation of colistin resistance in vitro." Clinical Microbiology and Antimicrobial Chemotherapy 27, no. 1 (2025): 88. https://doi.org/10.36488/cmac.2025.1.88-93.

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Objective. To describe the phenotypic and genome characteristics of the phenomenon of aztreonam susceptibility changes in a clinical Pseudomonas aeruginosa isolate developing colistin resistance in an evolutionary experiment in vivo. Materials and Methods. A clinical P. aeruginosa isolate with a multiple drug resistance phenotype was examined using the spaciotemporal model of colistin resistance development under the increasing colistin concentration. During the 44-day experiment, isolates were selected from the frontal growing line; these isolates were subjected to antimicrobial susceptibilit

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Obaid, Sarah Ahmed, and Rana Mujahid Al Shwaikh. "Evaluation the Efficacy of Bacteriophage Against Pseudomonas Aeruginosa Isolated from Wound and Burn Infections." Pakistan Journal of Medical and Health Sciences 16, no. 4 (2022): 440–44. http://dx.doi.org/10.53350/pjmhs22164440.

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One Hundred clinical samples were collected from different skin infection, including wounds and burns from patients in several hospitals in Baghdad governorate. The phenotypic diagnosis of isolated was performed by culturing on MacConkey agar medium and selective cetrimide agar. After a microscopic and biochemical tests diagnosis, the diagnosis was confirmed using Viteck2 system, 42 bacterial isolates were isolated belonging to P. aeruginosa, with ages ranging from (4 days-75 years). The results of sensitivity test with the Kirby–Bauer Disk diffusion technique found that 19 bacterial isolates

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Qin, Xuan, Danielle M. Zerr, Michael A. McNutt, Jessica E. Berry, Jane L. Burns, and Raj P. Kapur. "Pseudomonas aeruginosa Syntrophy in Chronically Colonized Airways of Cystic Fibrosis Patients." Antimicrobial Agents and Chemotherapy 56, no. 11 (2012): 5971–81. http://dx.doi.org/10.1128/aac.01371-12.

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ABSTRACTPseudomonas aeruginosaisolates from cystic fibrosis (CF) patients undergo remarkable phenotypic divergence over time, including loss of pigmentation, hemolysis, motility, and quorum sensing and emergence of antibiotic hypersusceptibility and/or auxotrophism. With prolonged antibiotic treatment and steady decline in lung function in chronically infected patients, the divergent characteristics associated with CF isolates have traditionally been regarded as “adapted/unusual virulence,” despite the degenerative nature of these adaptations. We examined the phenotypic and genotypic diversity

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Khan, Fazlurrahman, Min-Gyun Kang, Du-Min Jo, et al. "Phloroglucinol-Gold and -Zinc Oxide Nanoparticles: Antibiofilm and Antivirulence Activities towards Pseudomonas aeruginosa PAO1." Marine Drugs 19, no. 11 (2021): 601. http://dx.doi.org/10.3390/md19110601.

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With the advancement of nanotechnology, several nanoparticles have been synthesized as antimicrobial agents by utilizing biologically derived materials. In most cases, the materials used for the synthesis of nanoparticles from natural sources are extracts. Natural extracts contain a wide range of bioactive components, making it difficult to pinpoint the exact component responsible for nanoparticle synthesis. Furthermore, the bioactive component present in the extract changes according to numerous environmental factors. As a result, the current work intended to synthesize gold (AuNPs) and zinc

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Sabra, W., H. Lünsdorf, and A. P. Zeng. "Alterations in the formation of lipopolysaccharide and membrane vesicles on the surface of Pseudomonas aeruginosa PAO1 under oxygen stress conditions." Microbiology 149, no. 10 (2003): 2789–95. http://dx.doi.org/10.1099/mic.0.26443-0.

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It has been postulated that phenotypic variation in the relative expression of two chemically distinct types of lipopolysaccharide (LPS), a serotype-specific LPS (B-band) and a common antigen LPS (A-band) in Pseudomonas aeruginosa is an important mechanism enabling this opportunistic pathogen to alter its surface characteristics to mediate adhesion and to survive under extreme conditions. To further investigate this, the relative expression levels of the two distinct types of LPS in P. aeruginosa PAO1 were investigated with cells grown in a chemostat at different dissolved oxygen tensions (pO2

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Qader, Govend Musa, Khanzad Khudhur Jarjees, and Rozhhalat Khudhur Jarjees. "Molecular detection of Metallo-Beta-Lactamase and alginate in multidrug resistance Pseudomonas aeruginosa isolated from the clinical specimen." Journal of Medicine and Life 15, no. 9 (2022): 1105–9. http://dx.doi.org/10.25122/jml-2021-0196.

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Pseudomonas aeruginosa pathogen is opportunistic. Several virulence factors and biofilms can cause its pathogenicity. Furthermore, infections triggered via multidrug-resistant P. aeruginosa among hospitalized patients are a public health concern. The primary antimicrobial agents in treating Gram-negative infection include Meropenem and Imipenem. Moreover, the spread of Carbapenem-resistant P. aeruginosa is a focal concern worldwide. The present research aims to determine the spread of Carbapenem-resistant P. aeruginosa, and the distribution of the Alginate and Metallo-beta-lactamase encoding g

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Bauernfeind, A., I. Stemplinger, R. Jungwirth, R. Wilhelm, and Y. Chong. "Comparative characterization of the cephamycinase blaCMY-1 gene and its relationship with other beta-lactamase genes." Antimicrobial Agents and Chemotherapy 40, no. 8 (1996): 1926–30. http://dx.doi.org/10.1128/aac.40.8.1926.

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A plasmidic beta-lactamase which hydrolyzed cephamycins was first detected and reported in 1989. At that time its description was restricted to phenotypic characteristics. We analyzed nucleotide sequence of its gene and explored it genetic relationship with other bla genes. The deduced amino acid sequence of the blaCMY-1 product was compared with those of other known plasmidic cephamycinases and of chromosomal AmpC beta-lactamases. The results indicate that the relationship of CMY-1 is closest to MOX-1 among the plasmidic cephamycinases and to AmpC of Pseudomonas aeruginosa among the chromosom

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McMullen, Allison R., Melanie L. Yarbrough, Meghan A. Wallace, Angela Shupe, and Carey-Ann D. Burnham. "Evaluation of Genotypic and Phenotypic Methods to Detect Carbapenemase Production in Gram-Negative Bacilli." Clinical Chemistry 63, no. 3 (2017): 723–30. http://dx.doi.org/10.1373/clinchem.2016.264804.

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Abstract BACKGROUND Carbapenemase-producing gram-negative bacteria (CP-GNB) are an urgent and expanding public health threat. Rapid and accurate identification of these organisms facilitates infection prevention efforts in healthcare facilities. The objective of our study was to evaluate methods to detect and identify CP-GNB. METHODS We examined 189 carbapenem-resistant GNB(CR-GNB), including Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii complex, using 3 different methods: 2 methods to screen isolates of GNB for carbapenemase production [the carbapenem inactivation me

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Akinduti, Paul A., Onome W. George, Hannah U. Ohore, et al. "Evaluation of Efflux-Mediated Resistance and Biofilm formation in Virulent Pseudomonas aeruginosa Associated with Healthcare Infections." Antibiotics 12, no. 3 (2023): 626. http://dx.doi.org/10.3390/antibiotics12030626.

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Pseudomonas aeruginosa is a significant pathogen identified with healthcare-associated infections. The present study evaluates the role of biofilm and efflux pump activities in influencing high-level resistance in virulent P. aeruginosa strains in clinical infection. Phenotypic resistance in biotyped Pseudomonas aeruginosa (n = 147) from diagnosed disease conditions was classified based on multiple antibiotic resistance (MAR) indices and analysed with logistic regression for risk factors. Efflux pump activity, biofilm formation, and virulence factors were analysed for optimal association in Ps

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Gottfredsson, Magnús, Helga Erlendsdóttir, Ásbjörn Sigfússon, and Sigurdur Gudmundsson. "Characteristics and Dynamics of Bacterial Populations during Postantibiotic Effect Determined by Flow Cytometry." Antimicrobial Agents and Chemotherapy 42, no. 5 (1998): 1005–11. http://dx.doi.org/10.1128/aac.42.5.1005.

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ABSTRACT Changes in bacterial ultrastructure after antibiotic exposure and during the postantibiotic effect (PAE) have been demonstrated by electron microscopy (EM). However, EM is qualitative and subject to individual interpretation. In contrast, flow cytometry gives qualitative and quantitative information. The sizes and nucleic acid contents of Escherichia coli and Pseudomonas aeruginosa were studied during antimicrobial exposure as well as during the PAE period by staining the organisms with propidium iodide and analyzing them with flow cytometry and fluorescence microscopy. The effects of

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Shadrina, V. V., S. A. Krasovsky, E. K. Zhekaite, E. I. Kondratyeva, and E. G. Furman. "Clinical characteristics of the pathogenic variant L138ins of the CFTR gene in patients with cystic fibrosis." Voprosy praktičeskoj pediatrii 17, no. 3 (2022): 113–19. http://dx.doi.org/10.20953/1817-7646-2022-3-113-119.

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In this study, we analyzed phenotypic manifestations and clinical course of cystic fibrosis (CF) in patients with the pathogenic variant L138ins of the CFTR gene. The L138ins variant is rarely detected in CF patients worldwide, but is quite common in different regions of the Russian Federation, particularly in the Middle Urals. Before the neonatal screening implementation, the majority of CF patients with the L138ins variant were diagnosed late because of the mild disease course and dubious results of sweat tests. Such patients usually have elevated levels of immunoreactive trypsinogen (IRT) i

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Kirisits, Mary Jo, Lynne Prost, Melissa Starkey, and Matthew R. Parsek. "Characterization of Colony Morphology Variants Isolated from Pseudomonas aeruginosa Biofilms." Applied and Environmental Microbiology 71, no. 8 (2005): 4809–21. http://dx.doi.org/10.1128/aem.71.8.4809-4821.2005.

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ABSTRACT In this study, we report the isolation of small, rough, strongly cohesive colony morphology variants from aging Pseudomonas aeruginosa PAO1 biofilms. Similar to many of the P. aeruginosa colony morphology variants previously described in the literature, these variants autoaggregate in liquid culture and hyperadhere to solid surfaces. They also exhibit increased hydrophobicity and reduced motility compared to the wild-type parent strain. Despite the similarities in appearance of our colony morphology variant isolates on solid medium, the isolates showed a range of responses in various

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Bocharova, Yu A., T. A. Savinova, N. A. Mayansky, and Igor V. Chebotar. "New mutations in genes associated with cefiderocol resistance in a clinical isolate of Pseudomonas aeruginosa." Clinical Microbiology and Antimicrobial Chemotherapy 25, no. 4 (2023): 401–7. http://dx.doi.org/10.36488/cmac.2023.4.401-407.

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Objective. To assess the effects of chromosomal mutations on emergence of cefiderocol resistance among Pseudomonas aeruginosa clinical isolates. Materials and Methods. Study design purported to compare the characteristics of phenotypic antibiotic resistance and chromosomal mutations of P. aeruginosa strains of a common origin possessing different resistance levels to cefiderocol. Two P. aeruginosa isolates from the sputum of a patient with cystic fibrosis who had not previously received cefiderocol were analyzed. Species identification was performed using an MALDI-TOF MS instrument and whole g

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Bobai, Mathew, Lawal Danjuma, Nura Muhammad Sani, Joshua Istifanus Anekoson, and Yusuf Nuhu. "Correlation Between Patients with Wounds and Isolated Pseudomonas aeruginosa and Staphylococcus aureus at Barau Dikko Teaching Hospital, Kaduna, Nigeria." Journal of Biochemistry, Microbiology and Biotechnology 10, no. 2 (2022): 35–41. http://dx.doi.org/10.54987/jobimb.v10i2.755.

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This study was carried out to determine the correlation between patients with wounds and isolated Pseudomonas aeruginosa and Staphylococcus aureus in clinics at Barau Dikko Teaching Hospital, Kaduna, Nigeria. The Socio-demographic, medical and drug histories and characteristics of the wounds from each consented patient were taken using a questionnaire along with sixty samples of the patient’s wound swab samples. Isolation of Pseudomonas aeruginosa and Staphylococcus aureus from the wound swab samples was carried out using standard phenotypic and genotypic procedures. Out of the 60 samples coll

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Phankhajon, Kanchariya, Anchana Somdee, and Theerasak Somdee. "Algicidal activity of an actinomycete strain, Streptomyces rameus, against Microcystis aeruginosa." Water Science and Technology 74, no. 6 (2016): 1398–408. http://dx.doi.org/10.2166/wst.2016.305.

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An actinomycete strain (KKU-A3) with algicidal activity against Microcystis aeruginosa was isolated from soil in Khon Kaen Province, Thailand. Based on its phenotypic characteristics and 16S rDNA sequence, strain KKU-A3 was identified as Streptomyces rameus. Strain KKU-A3 also exhibited algicidal activity against the cyanobacteria Synechococcus elongatus, Cylindrospermum sp. and Oscillatoria sp. A mathematical and statistical technique was used to optimize the culture conditions and maximize its anti-Microcystis activity. The single factor experiments indicated that glucose and casein were the

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Sukhada, Buwa, Ingale Hemangi, Bawane Rashmi, Bhandari Sunita, and Sengupta Shubhra. "Antimicrobial Susceptibility Pattern of Pseudomonas aeruginosa Isolates in Clinical Samples with Special Reference to Metallo Beta Lactamase Detection at a Tertiary Care Hospital." International Journal of Toxicological and Pharmacological Research 13, no. 1 (2023): 179–85. https://doi.org/10.5281/zenodo.11311709.

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<strong>Introduction:</strong> In recent years, a significant rise in the incidence of <em>P. aeruginosa</em> as well as multidrug resistance (MDR) has been seen, which has been accompanied by an increase in morbidity and death. The purpose of this study was to ascertain the current status of antimicrobial susceptibility to anti-Pseudomonal drugs and to identify the presence of metallo- beta lactamase (MBL) in these isolates. <strong>Material and Methods: </strong>A prospective study was carried out to obtain <em>P. aeruginosa</em> isolates from various clini

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Evangelista, Leones Fernandes, Ana Leilania Freitas Vasconcelos, Marcus Vinícius Saldanha Ribeiro, et al. "Detection of Carbapenemases in Pseudomonas aeruginosa Isolates: An Emerging Challenge." Microbiology Research Journal International 34, no. 8 (2024): 36–44. http://dx.doi.org/10.9734/mrji/2024/v34i81469.

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Aims: Determine the clinical characteristics of patients and the microbiological characteristics of the Pseudomonas aeruginosa isolates in respiratory samples from Adult Intensive Care Unity (ICU) of a University Hospital from Fortaleza, Brazil; Analyze the resistance profile of Pseudomonas aeruginosa isolates; Determine the phenotypic prevalence of Carbapenem-Resistant Pseudomonas aeruginosa (CRPA); Relate the prevalence to resistent Pseudomonas aeruginosa with patients’ death rate. Study Design: This is a epidemiological, descriptive and retrospective study, carried out between January and D

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Alliouch-Kerboua, Chérifa, Ghania Bourzama, Djamila Gacemi-Kirane, and Bernard La Scola. "Molecular identification, antibacterial activity, and production of hydrolytic enzymes by halotolerant bacterium Streptomyces sp. ESM2-25 GTF strain isolated from extreme environment in north-east of Algeria." Nova Biotechnologica et Chimica 22, no. 1 (2023): e1429. http://dx.doi.org/10.34135/nbc.1429.

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The aim of this study is to establish the taxonomic position of the halotolerant bacterium ESM2-25 GTF strain isolated from the extreme environment of El-Mellah lagoon water, which is situated in the city of El-Kala in the northeast of Algeria and to study its phenotypic characteristics, antibacterial activity against several pathogenic bacteria, and hydrolytic enzymes production. The novel bacterium ESM2-25 GTF strain was isolated from water samples by the dilution agar plating method using the starch-casein medium, screened in vitro for its hydrolytic enzymes production and antibacterial act

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Wang, Xuejie, Carmen Villa, Yadira Dobarganes, et al. "Systemic Inflammatory Biomarkers Define Specific Clusters in Patients with Bronchiectasis: A Large-Cohort Study." Biomedicines 10, no. 2 (2022): 225. http://dx.doi.org/10.3390/biomedicines10020225.

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Differential phenotypic characteristics using data mining approaches were defined in a large cohort of patients from the Spanish Online Bronchiectasis Registry (RIBRON). Three differential phenotypic clusters (hierarchical clustering, scikit-learn library for Python, and agglomerative methods) according to systemic biomarkers: neutrophil, eosinophil, and lymphocyte counts, C reactive protein, and hemoglobin were obtained in a patient large-cohort (n = 1092). Clusters #1–3 were named as mild, moderate, and severe on the basis of disease severity scores. Patients in cluster #3 were significantly

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Abraham, E., A. A. Freitas, and A. A. Coutinho. "Purification and characterization of intraparenchymal lung lymphocytes." Journal of Immunology 144, no. 6 (1990): 2117–22. http://dx.doi.org/10.4049/jimmunol.144.6.2117.

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Abstract We analyzed phenotypic and functional characteristics of intraparenchymal pulmonary lymphocytes in mice. As determined by flow cytometry, approximately 30% more T cells than B cells were found. Nearly all T cells bore the alpha beta TCR, most of which stained with either CD4+ or CD8+, although small numbers of double-negative T cells were present; the CD8+/CD4+ ratio was approximately 0.37. While practically all B cells bore surface IgM, and no IgA+ cells were found, and approximately more than 80% of the plasma cells produced IgA. Approximately half of the LPS-responsive cells produc

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Gill, Christian M., Tomefa E. Asempa, and David P. Nicolau. "656. Development and Application of a Pragmatic Algorithm for the Detection of Carbapenemase-Producing Pseudomonas aeruginosa (CP-PA)." Open Forum Infectious Diseases 7, Supplement_1 (2020): S384—S385. http://dx.doi.org/10.1093/ofid/ofaa439.849.

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Abstract Background Historically, carbapenem-resistance in P. aeruginosa (PA) has been mediated by inducible AmpC, drug efflux, and porin loss; however, carbapenemase production is an increasingly recognized entity. Of these mechanisms, carbapenemases can drastically reduce treatment options and rapidly disseminate. Since broad applications of phenotypic (mCIM/eCIM) and PCR-based detection can be labor intensive and costly, we developed an MIC derived algorithm to streamline use of these definitive carbapenemase detection methodologies. Methods To develop the testing criteria, a challenge set

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Borisova, Dayana, Tanya Strateva, Svetoslav G. Dimov, et al. "Diversification of Pseudomonas aeruginosa After Inhaled Tobramycin Therapy of Cystic Fibrosis Patients: Genotypic and Phenotypic Characteristics of Paired Pre- and Post-Treatment Isolates." Microorganisms 13, no. 4 (2025): 730. https://doi.org/10.3390/microorganisms13040730.

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This study examines the impact of inhaled tobramycin therapy on the within-host changes in P. aeruginosa strains isolated from Bulgarian patients with CF prior to and post treatment. Genotypic comparison by RAPD-PCR indicated that most of the pre-treatment isolates had a high similarity and were genetically comparatively close to strains from other countries with known increased morbidity or treatment requirements. Most of the post-treatment isolates were, however, genetically distant from their pre-treatment counterparts, showing genotypic diversification after the treatment. Phenotypic compa

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Garza-González, Elvira, Paola Bocanegra-Ibarias, Miriam Bobadilla-del-Valle, et al. "Drug resistance phenotypes and genotypes in Mexico in representative gram-negative species: Results from the infivar network." PLOS ONE 16, no. 3 (2021): e0248614. http://dx.doi.org/10.1371/journal.pone.0248614.

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Aim This report presents phenotypic and genetic data on the prevalence and characteristics of extended-spectrum β-lactamases (ESBLs) and representative carbapenemases-producing Gram-negative species in Mexico. Material and methods A total of 52 centers participated, 43 hospital-based laboratories and 9 external laboratories. The distribution of antimicrobial resistance data for Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae complex, Acinetobacter baumannii complex, and Pseudomonas aeruginosa in selected clinical specimens from January 1 to March 31, 2020 was analyzed using the W

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Lau, Peter C. Y., Theresa Lindhout, Terry J. Beveridge, John R. Dutcher, and Joseph S. Lam. "Differential Lipopolysaccharide Core Capping Leads to Quantitative and Correlated Modifications of Mechanical and Structural Properties in Pseudomonas aeruginosa Biofilms." Journal of Bacteriology 191, no. 21 (2009): 6618–31. http://dx.doi.org/10.1128/jb.00698-09.

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ABSTRACT Bacterial biofilms are responsible for the majority of all microbial infections and have profound impact on industrial and geochemical processes. While many studies documented phenotypic differentiation and gene regulation of biofilms, the importance of their structural and mechanical properties is poorly understood. Here we investigate how changes in lipopolysaccharide (LPS) core capping in Pseudomonas aeruginosa affect biofilm structure through modification of adhesive, cohesive, and viscoelastic properties at an early stage of biofilm development. Microbead force spectroscopy and a

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Jamasbi, Roudabeh J., and Eric M. Proudfoot. "Phenotypic and Genotypic Characteristics of Clinical Isolates of Pseudomonas aeruginosa: Rate of Occurrence and Distribution of Different Serotypes, Antimicrobial Susceptibility Profiles, and Molecular Typing." Laboratory Medicine 39, no. 3 (2008): 155–61. http://dx.doi.org/10.1309/1baww0951n7v71ce.

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Howard-Anderson, Jessica, Chris W. Bower, Gillian Smith, Sarah W. Satola та Jesse T. Jacob. "834. Clinical Outcomes with Carbapenem-Resistant Pseudomonas aeruginosa that Retain Susceptibility to Traditional Antipseudomonal β-lactams: Atlanta, 2016-2018". Open Forum Infectious Diseases 7, Supplement_1 (2020): S457—S458. http://dx.doi.org/10.1093/ofid/ofaa439.1023.

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Abstract Background Carbapenem-resistant Pseudomonas aeruginosa (CRPA) often results from multiple mechanisms, creating unique phenotypic patterns of resistance including retaining susceptibility to traditional antipseudomonal β-lactams: cefepime (FEP), ceftazidime (CAZ) and piperacillin-tazobactam (TZP). Outcomes of patients with CRPA susceptible to FEP, CAZ and TZP are unclear. Methods The Georgia Emerging Infections Programed performs active, population-based surveillance for CRPA (minimum inhibitory concentration [MIC] ≥ 8 µg/mL for doripenem, imipenem or meropenem) isolated from sterile s

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Lukyanenko, N. V., K. I. Sursyakova, T. V. Safyanova, V. V. Prokopyev, and A. A. Russkikh. "Comparative Aspects of Phenotypic and Genotypic Characteristics of Strains of ISMP Pathogens Isolated from Patients of a Large Multidisciplinary Hospital." Medicina 13, no. 1 (2025): 33–46. https://doi.org/10.29234/2308-9113-2024-13-1-33-46.

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Introduction: In recent decades, Russia, like the rest of the world, has seen a rapid increase in antimicrobial resistance (AMR) among pathogens causing infectious diseases. The development of drug resistance leads to microorganisms' ability to survive despite the use of etiotropic therapy. Alongside multidrug resistance (MDR), hypervirulence – associated with the acquisition of additional genetic material and the emergence of new genetic lineages – is becoming increasingly significant. The purpose of the study: To conduct a comparative phenotypic and genotypic assessment of the main pathogens

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Bilton, D., A. Pye, MM Johnson, et al. "The isolation and characterization of non-typeable Haemophilus influenzae from the sputum of adult cystic fibrosis patients." European Respiratory Journal 8, no. 6 (1995): 948–53. http://dx.doi.org/10.1183/09031936.95.08060948.

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The role of non-typeable Haemophilus influenzae in cystic fibrosis (CF) remains unclear. We wanted, therefore, to determine the presence and characteristics of non-typeable H. influenzae in sputum samples from patients with CF. In order to do this, we have assessed sputum samples from 55 consecutive clinically stable patients seen routinely at an adult CF out-patient clinic. Quantitative bacterial culture was performed using a selective media containing cefsoludin, and isolates were characterized by biotyping and outer membrane protein profile analysis. In 17 (30%) of these samples, non-typeab

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Barbosa, Thaís Alves, Maria Regina Bentlin, Lígia Maria Suppo de Souza Rugolo, et al. "Molecular Characterization of Gram-Negative Bacilli Isolated from a Neonatal Intensive Care Unit and Phenotypic and Molecular Detection of ESBL and Carbapenemase." Antibiotics 14, no. 4 (2025): 342. https://doi.org/10.3390/antibiotics14040342.

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Introduction: The increase in the rates of multidrug-resistant bacteria in healthcare environments has been recognized as a global public health problem. In view of the scarcity of data on the neonatal population, this study aimed to provide information on the genotypic and epidemiological characteristics of Gram-negative microorganisms isolated from colonization and infection sites in neonates admitted to a tertiary university center of high complexity. Methods: Enterobacterales and non-fermenting Gram-negative bacilli previously collected in a prospective cohort study were submitted to genot

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Starkey, Melissa, Jason H. Hickman, Luyan Ma, et al. "Pseudomonas aeruginosa Rugose Small-Colony Variants Have Adaptations That Likely Promote Persistence in the Cystic Fibrosis Lung." Journal of Bacteriology 191, no. 11 (2009): 3492–503. http://dx.doi.org/10.1128/jb.00119-09.

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ABSTRACT Pseudomonas aeruginosa is recognized for its ability to colonize diverse habitats, ranging from soil to immunocompromised people. The formation of surface-associated communities called biofilms is one factor thought to enhance colonization and persistence in these diverse environments. Another factor is the ability of P. aeruginosa to diversify genetically, generating phenotypically distinct subpopulations. One manifestation of diversification is the appearance of colony morphology variants on solid medium. Both laboratory biofilm growth and chronic cystic fibrosis (CF) airway infecti

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