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Artykuły w czasopismach na temat „Agar well diffusion assay”

Autor: Grafiati

Data publikacji: 3 czerwca 2025

Data aktualizacji: 31 lipca 2025

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1

Esra, Demir, and Başbülbül Gamze. "Screening of Bacteriocin Production in Lactic Acid Bacteria Isolated From Fermented Dairy Products." Biotechnology Journal International 18, no. 2 (2017): 1–9. https://doi.org/10.9734/BJI/2017/33504.

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<strong>Aims: </strong>In this study we aimed to screen LAB strains from different fermented dairy products by phenotypic and genotypic methods. <strong>Methodology:</strong> 138 LAB isolates belonging to <em>Enterococcus</em> (13,76%), <em>Lactococcus</em> (18,84%) and <em>Lactobacillus</em> (67,39%) genera from fermented dairy products were used to search for their bacteriocin production and bacteriocin encoding genes were searched. LAB isolates were screened by spot-on lawn assay method against indicator microorganisms. <em>Geobacillus stearothermophilus</em> DSMZ 22, <em>Escherichia coli</

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Chigozie, Celestina Ezeagha, Ogechukwu Ogbuebuna Jacinta, Nwanyioma Anozie Amara, Chuks Oranu Emmanuel, and Henrietta Nedum Chinenye. "Determination of the Phytochemical and Antimicrobial properties of Nauclea latifolia Root Smith. (Rubiaceae)." GSC Biological and Pharmaceutical Sciences 23, no. 2 (2023): 182–88. https://doi.org/10.5281/zenodo.8041513.

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<em>Nauclea latifolia </em>is a valuable medicinal plant for treating different ailments. Infectious diseases constitute a world health concern. This work describes the phytochemical analysis and antimicrobial properties of the root of <em>N. latifolia</em>. Collected root samples were extracted via cold maceration with methanol. The methanol crude extract was fractionated into n-hexane, ethyl acetate, butanol and aqueous fractions. Phytochemical analyses and antimicrobial activity of the methanol extract/ fractions were assessed using agar well diffusion method. Four different fract

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Loureno, Felipe Rebello, Telma Mary Kaneko, and Terezinha De Jesus Andreoli Pinto. "Validation of Erythromycin Microbiological Assay Using an Alternative Experimental Design." Journal of AOAC INTERNATIONAL 90, no. 4 (2007): 1107–10. http://dx.doi.org/10.1093/jaoac/90.4.1107.

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Abstract The agar diffusion method, widely used in antibiotic dosage, relates the diameter of the inhibition zone to the dose of the substance assayed. An experimental plan is proposed that may provide better results and an indication of the assay validity. The symmetric or balanced assays (2 2) as well as those with interpolation in standard curve (5 1) are the main designs used in the dosage of antibiotics. This study proposes an alternative experimental design for erythromycin microbiological assay with the evaluation of the validation parameters of the method referring to linearity, precis

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4

Zainab, Kashmala, and Hira Batool. "A Simplistic Screening Assay of Antimicrobial Compounds and Enzymatic Activity from Local Soil Microbes." RADS Journal of Biological Research & Applied Sciences 9, no. 1 (2018): 24–29. http://dx.doi.org/10.37962/jbas.v9i1.118.

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Antibiotics production is the most emerging field worldwide with a constant need for the new ones to fight the microbial resistance. In this context, the research was pursued to isolate, characterize and screen for promising antibiotic-producing microbes from local soil. The soil bacterial isolates (S1, S2, S3,S4, and S5) and fungal isolates (F1, F2, F4, F6, and F7) were selected and screened for antimicrobial activity against the Test bacteria by agar well diffusion and disc diffusion methods. Assays for Extracellular enzymes including Protease, Lipase, Lecithinase, Cellulase, and Amylase fol

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5

Şentürk, Esra, Sema Yakıt, and Pınar Şanlıbaba. "Antilisterial Activity by Enterococcus Species Isolated from Traditional Cheeses." Turkish Journal of Agriculture - Food Science and Technology 8, no. 7 (2020): 1593–97. http://dx.doi.org/10.24925/turjaf.v8i7.1593-1597.3503.

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In this study, it was aimed to detect the antimicrobial activity of 312 Enterococcus species against Listeria monocytogenes. Antimicrobial activity was detected by agar spot and well diffusion assay. A total of 201 enterococcal strains inhibited the growth of L. monocytogenes strains based on the agar spot test. Only 44 strains showed antimicrobial activity against L. monocytogenes strains using agar well diffusion assay. Of the 44 enterococcal strains screened, 6 E. faecium (2.99%) strains had a high antimicrobial effect against indicator L. monocytogenes strains. The antilisterial activity o

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6

Saranya, Shankar, and Sathiavelu Mythili. "Endophytic fungus - Albifimbria verrucaria isolated from Centella asiatica and its antioxidant and antibacterial activities." Research Journal of Biotechnology 20, no. 2 (2024): 92–98. https://doi.org/10.25303/202rjbt092098.

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The present research focuses on the isolation of endophytic fungi from the roots of Centella asiatica and analyzes the antagonistic efficiency of endophytic fungus against bacterial fish pathogens. Agar plug diffusion assay, phytochemical screening, antioxidant activity, agar well diffusion assay and minimum inhibitory and bactericidal concentration were done for the fungal endophytes. Eight endophytic fungi in total were isolated from C.asiatica roots. Among that, CAR1 exhibits maximum antibacterial action in the preliminary screening and it is subjected to an extraction procedure using diffe

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7

King, Thea, Gary Dykes, and Ruth Kristianti. "Comparative Evaluation of Methods Commonly Used to Determine Antimicrobial Susceptibility to Plant Extracts and Phenolic Compounds." Journal of AOAC INTERNATIONAL 91, no. 6 (2008): 1423–29. http://dx.doi.org/10.1093/jaoac/91.6.1423.

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Abstract A comparison was made to evaluate the ability of the most commonly used qualitative agar diffusion methods and a quantitative broth dilution assay to determine the antimicrobial activity of a plant extract and a variety of phenolic compounds. A disc and well diffusion technique and a microtiter broth microdilution (MBM) assay were used as antimicrobial susceptibility tests of a plant extract and several phenolic compounds against 7 bacterial species. In both the well and disc diffusion assays, the level of reproducibility was poor and a linear or logarithmic relationship did not exist

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8

Eissa, Dalia Essam, Engy Refaat Rashed, and Mostafa Essam Eissa. "Suitability System of Microbiological Method for Nystatin Potency Determination in the Routine Analysis Using Agar Diffusion Method." SciMedicine Journal 3, no. 4 (2021): 302–15. http://dx.doi.org/10.28991/scimedj-2021-0304-2.

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Nystatin is a polyene macrolide antifungal active which is used for the treatment of candidiasis and obtained from some species of Streptomycesbacteria. The present work describes the statistical suitability analysis for regular monitoring of the agar diffusion bioassay in a simple, inexpensive and time-saving process before potency determination. A balanced (symmetrical) two-dose parallel line assay model was applied using the agar well diffusion method for quantification of Nystatin in raw material and finished medicinal dosage form. The routine inspection methodology yielded good results an

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9

Gummuluri, Sriram, Venkata Teja Kavalipurapu, and Apoorva Vasundhara Kaligotla. "Antimicrobial efficacy of Novel Ethanolic Extract of Morinda Citrifolia Against Enterococcus Feacalis by Agar Well Diffusion Method and Minimal Inhibitory Concentration- An Invitro Study." Brazilian Dental Science 22, no. 3 (2019): 365–70. http://dx.doi.org/10.14295/bds.2019.v22i3.1731.

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Introduction: the long term success of root canal treatment is ultimately related to the effective debridement and disinfection of the root canal system. Hence, the irrigants play an important role in achieving the good penetrability and bactericidal activity. The present study was mainly aimed at evaluating the invitro antimicrobial efficacy of Novel Ethanolic Extract of Morinda Citrifolia by agar well diffusion and minimal inhibitory concentration. Materials and Methods:the antimicrobial efficacy of the Novel Ethanolic Extract of Morinda Citrifolia was tested using agar well diffusion and mi

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10

Nalini, T. J., H. N. Ramesh Babu, N. Rajeshwari, K. Y. Prathibha, and E. Keshamma. "Assay of Antimicrobial Activity of Sida acuta (Burm. F.) by Well Diffusion Method." International Journal of Current Microbiology and Applied Sciences 11, no. 9 (2022): 183–89. http://dx.doi.org/10.20546/ijcmas.2022.1109.021.

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Sida acuta is a shrub indigenous to pantropical regions. Sida acuta belongs to the mallow family, Malvaceae. The plant is widely used for its various pharmacological properties. Therefore, in the current study antifungal activity for leaf, stem and root extracts of Sida acuta were carried out. Ethanol (50%) was used successively for extraction of active principles from the dried powdered leaves, stem and root. The antifungal screening was done with two plant pathogens viz. Claviceps purpurea MTCC 2334 and Macrophemina phaseolina MTCC 10576 as test microorganisms. In the agar-well diffusion ass

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11

Donga, Savan, Pooja Moteriya, and Sumitra Chanda. "EVALUATION OF ANTIMICROBIAL AND SYNERGISTIC ANTIMICROBIAL PROPERTIES OF PTEROCARPUS SANTALINUS." Asian Journal of Pharmaceutical and Clinical Research 10, no. 11 (2017): 204. http://dx.doi.org/10.22159/ajpcr.2017.v10i11.20939.

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Objectives: The aim of the present study was to evaluate antibacterial and synergistic antimicrobial properties of leaf, stem and bark of Pterocarpus santalinus. Methods: The extraction was done by decoction method. The antimicrobial activity was done by agar well diffusion assay and the synergistic antimicrobial activity was done by agar disc diffusion assay. Results: The synergistic activity was studied with plant extracts plus antibiotics viz. Ampicillin , Polymyxin-B, Clotrimazole and Fluconazole. Conclusions: Amongst the three parts, the best antimicrobial activity was shown by bark extra

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12

Prastiyanto, Muhammad Evy, Fandhi Adi Wardoyo, Wildiani Wilson, and Sri Darmawati. "Antibacterial Activity of Various Extracts of Averrhoa bilimbi against Multidrug Resistant Bacteria." Biosaintifika: Journal of Biology & Biology Education 12, no. 2 (2020): 163–68. http://dx.doi.org/10.15294/biosaintifika.v12i2.23600.

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The multi-drug resistance (MDR) bacteria is a global health problem that causes high mortality every year. Therefore, novel antibacterial agents are needed from natural biological sources. This research aimed to investigate the antibacterial activities of various crude extracts of Averrhoa bilimbi against MDR bacteria. The antibacterial activity was calculated based on the use agar well diffusion assay and the minimum bactericidal concentration (MBC) using Mueller–Hinton broth in a microdilution method. Bacteria from wells were subcultured using inoculating loop onto a 5% sheep BAP. The best a

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13

Ortlieb, Nico, Elke Klenk, Andreas Kulik, and Timo Horst Johannes Niedermeyer. "Development of an agar-plug cultivation system for bioactivity assays of actinomycete strain collections." PLOS ONE 16, no. 11 (2021): e0258934. http://dx.doi.org/10.1371/journal.pone.0258934.

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Natural products are an important source of lead compounds for the development of drug substances. Actinomycetes have been valuable especially for the discovery of antibiotics. Increasing occurrence of antibiotic resistance among bacterial pathogens has revived the interest in actinomycete natural product research. Actinobacteria produce a different set of natural products when cultivated on solid growth media compared with submersed culture. Bioactivity assays involving solid media (e.g. agar-plug assays) require manual manipulation of the strains and agar plugs. This is less convenient for t

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14

Suryawanshi, H. K., and N. D. Pandya. "Screening, Identification of Alkaline Proteases Producing Fungi from Soil of Different Habitats of Amalner Tahsil [Maharashtra] and Their Applications." International Journal of Applied Sciences and Biotechnology 5, no. 3 (2017): 397–402. http://dx.doi.org/10.3126/ijasbt.v5i3.18304.

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Fungal proteases had wide applications in textile, leather, food and Pharmaceutical industries. As proteases shows proteolytic activity they are helpful in proteinic stain removal hence also used in various commercial detergent industries. For fungal isolation soil samples were collected from different sites of Amalner tahsil. (Maharashtra) e.g. crop fields, near dairy areas, poultry farms etc. Those soil samples showing alkaline pH were selected for isolation of fungi on Potato Dextrose Agar plates. Then among 14 different isolates 2 were selected for their most proteolytic activity after scr

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Dar, Umair Ikram, Farooq Saleem, and Saad Touqeer. "Antimicrobial and antioxidant activity of Embelia robusta: a common adulterant in black pepper." Pakistan Journal of Pharmaceutical Research 2, no. 2 (2016): 136. http://dx.doi.org/10.22200/pjpr.20162136-141.

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The present study was aimed to investigate the phytochemistry as well as the antibacterial, antifungal, antioxidant and irritant activities of fruit of Embelia robusta. Agar-well diffusion method was used for antimicrobial study whereas for antioxidant activity three tests namely, DPPH assay, β-Carotene assay and ascorbic acid content were performed. The results from antibacterial assay showed presence of antibacterial activity in all the three plant extracts (methanol, chloroform and n-hexane). Significant antioxidant activity was also found to be present and plant extracts showed no irritant

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16

Prastiyanto, Muhammad Evy, Inas Hasna Azizah, Hafizha Dara Haqi, et al. "In-vitro antibacterial activity of the seed extract of three-member Artocarpus towards Methicillin-Resistant Staphylococcus aureus (MRSA)." Jurnal Teknologi Laboratorium 9, no. 2 (2020): 128–35. http://dx.doi.org/10.29238/teknolabjournal.v9i2.237.

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Methicillin-Resistant Staphylococcus aureus (MRSA) infections have created a critical need for the development of natural antibacterials from a biological source. This research aimed to investigate the antibacterial activity of the seed extract of three-member Artocarpus (Artocarpus heterophyllus, A. champeden, and A. camansi) against MRSA which are the most prevalent causes of infections in patients. Crude seed extracts of three-member Artocarpus were evaluated for their antibacterial activity against MRSA. The antibacterial activity against MRSA of the three extracts was assayed in vitro by

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Kannan, Kilavan Packiam, Imdhiyas Mohamed, Sabarivasan Kannadhasan, and Madhankumar Dhaksinamoorthy. "Studies on Antioxidants, Antibacterial activities and Phytochemistry of Mimusops elengi. Linn." Research Journal of Biotechnology 17, no. 3 (2022): 27–32. http://dx.doi.org/10.25303/1703rjbt2732.

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The present study involves screening of phytoconstituents in the ethanolic extract of Mimusops elengi. L leaves. The total phenolic content, antioxidant and antimicrobial activities of the same were determined. Mimusops elengi. L leaves were extracted with ethanol using Soxhlet apparatus. The total phenolic content was determined by Folin-Ciocalteu method. The antioxidant potential of the extract was evaluated by using 2, 2-diphenyl 1- picryl Hydrazyl radical (DPPH) scavenging assay and ferric reducing power (FRAP) assay. Antibacterial activity was assessed against four MTCC bacterial pathogen

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18

Athanassiadis, B., PV Abbott, N. George, and LJ Walsh. "Anin vitrostudy of the antimicrobial activity of some endodontic medicaments and their bases using an agar well diffusion assay." Australian Dental Journal 54, no. 2 (2009): 141–46. http://dx.doi.org/10.1111/j.1834-7819.2009.01107.x.

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NS, Shree Abiraami, Devika Pillai, and S. Rajeshkumar. "Comparison of Silver, Zinc Oxide, and Chitosan-mediated Nanoparticle synthesis and their antifungal activity against Oral Candidiasis." International Journal of Membrane Science and Technology 10, no. 4 (2023): 2295–302. http://dx.doi.org/10.15379/ijmst.v10i4.2411.

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Aim: To analyze the effectiveness of silver, zinc oxide, and chitosan nanoparticles against oral candidiasis. Materials and Methods: Agar-well diffusion method: After the synthesis of AgNP, ZnONP, and ChNP In the agar plate, wells are cut, and 25 l, 50 l, and 100 l concentrations of the solution are placed. Reading was taken after 24 hours. The zone of inhibition was noted. Time-kill curve assay: RBA broth was prepared and sterilized, and 6 mL was added to all five test tubes. Candidia suspension was added to all five test tubes in the range of 5×105 CFU/ml. The first three tubes contain silve

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Dorothy, P., and M. K. Mahesh. "Qualitative and Quantitative Analysis of Phytochemicals and Anti-microbial Activity of Madhuca bourdillonii (Gamble) Lam." Journal of Plant Science Research 38, no. 2 (2023): 663–81. http://dx.doi.org/10.32381/jpsr.2022.38.02.21.

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The aim of the present study was to investigate the qualitative and quantitative analysis of Madhuca bourdillonii extracts and demonstrate its biological activities. The results of phytochemical analysis showed that the leaf water extract consists of more amount of total phenolics and tannins respectively of 7.4 mg of gallic acid/g and 113.28 mg%. Flavonoid content was found to be more in water extract of bark amounting to 40.73 mg of quercetin/g while the acetone extract of leaves showed more amounts of alkaloids accounting to 30.25 mg%. All the extracts of leaves and stems were evaluated for

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Umaru, Kerenhappuch Isaac, Isaac John Umaru, Akafa Andes Tansaba, Chakfa Nanmar, and Egeonu Stephen Ugoeze. "Evaluation of Antibacterial Activity and Toxicity Effects of Newbouldia Stem Bark Extract." African Journal of Biochemistry and Molecular Biology Research 1, no. 1 (2024): 12–22. https://doi.org/10.58578/ajbmbr.v1i1.3350.

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Newbouldia leavis is a boundary tree. it is a medium size angiosperm which belongs to the Bignoniaceae family. It is a fast-growing evergreen shrub or small tree. This study was carried out to investigate antibacterial activity of the methanol extract of Newbouldia stem bark. The antibacterial activity of the methanol extracts was determined by the agar well diffusion method. Three standard bacterial strains: Escherichia coli, Staphylococcus aureus, and Klebsiella pneumoniae were used for the antibacterial assay. Result of screening plant extracts for antibacterial activity showed that most of

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Narvasa, Vanessa Grace, and Eddie Mondejar. "Phytochemical screening, antibacterial and cytotoxic activities of Aristolochia philippinensis Warb. leaf extracts." International Journal of Secondary Metabolite 12, no. 3 (2025): 537–47. https://doi.org/10.21448/ijsm.1521859.

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The Aristolochia philippinensis Warb. (Aristolochiaceae) is commonly used in traditional medicine among indigenous people in the Philippines. The leaves of A. philippinensis are used to treat illnesses such as stomach aches, bodily aches, and physical relapse. This study was conducted to identify the phytochemical compounds and evaluate the antibacterial and cytotoxic activity of A. philippinensis. The leaf extracts were subjected to qualitative phytochemical screening, in vitro testing for antibacterial activity using agar well-diffusion assay against selected gram-positive and gram-negative

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Dashti, Khadijah M., Leila Vali, Norya Almaraghi, Hussain Ebrahim, Hassan Abul, and Ali A. Dashti. "Antibiotic-Producing Bacteria Collected from Seawater." Microbiology Research 15, no. 4 (2024): 2381–93. http://dx.doi.org/10.3390/microbiolres15040160.

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Background: Microorganisms are a known source of antibiotics. The study aimed to identify and screen antibiotic-producing microbes isolated from seawater. Method: Three of the fifty (50) bacteria isolated from seawater showed positive for antibiotic activity. The antimicrobial activity of Pseudomonas guguanensis (KD1) was screened against the ESKAPE pathogens using agar-well diffusion assays. P. guguanensis (KD1) was selected for the fermentation and extraction of antimicrobial compounds using solvent extraction assays. Results: P. guguanensis (KD1) produced the highest antibacterial activity

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R. Ghanim, Russel, M. R. Mohammad, and Adi M. Abdul Hussien. "Antibacterial Activity and Morphological Characterization of Synthesis Graphene Oxide Nanosheets by Simplified Hummer’s Method." Biosciences, Biotechnology Research Asia 15, no. 3 (2018): 627–33. http://dx.doi.org/10.13005/bbra/2669.

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Graphene oxide (GO) nanosheets were prepared by a novel simplified Hummer's method. The morphological and cross section images of GO have been tested with field emission-scanning electron microscope (FE-SEM). The antibacterial activity of GO nansheets against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were investigated as a model for Gram-negative bacteria and Gram-positive bacteria respectively. Bacteriological tests were performed by agar well diffusion assay with different concentrations of GO nanosheets and the bacterial morphological change of two bacterial species h

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Vijayalakshmi, R., N. Ambalavanan, S. Rajesh Kumar, Jaideep Mahendra, Uma Sudhakar, and Tharani Munusamy. "Comparative analysis of antimicrobial efficacy and cytotoxicity of Terminalia arjuna bark extract mediated zinc oxide nanoparticles (ZnONPs) and selenium nanoparticles (SeNPs): An in-vitro study." IP International Journal of Periodontology and Implantology 9, no. 4 (2024): 188–94. https://doi.org/10.18231/j.ijpi.2024.039.

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This study provides an in-depth comparative analysis of the antimicrobial efficacy and cytotoxic effects of -mediated Selenium Nanoparticles (TA-SeNPs) and Zinc Oxide Nanoparticles (TA-ZnONPs) in the context of chronic periodontitis. The well diffusion assay and Brine Shrimp Lethality Assay were employed to assess antibacterial efficacy and cytotoxicity, respectively. The aim of the studyis to contribute to the understanding of the potential applications and safety considerations associated with green-synthesized ZnONPs and SeNPs as a local drug delivery agent in the treatment of chronic perio

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Odo, Kelvin Ejiofor, Matthias Onyebuchi Agbo, and Patience Ogoamaka Osadebe. "Extract and Fractions from Soil Bacteria (Streptomyces canus ATCC 12647) Possess Antimicrobial and Anti-Oxidative Potential in vitro." Jordan Journal of Pharmaceutical Sciences 15, no. 3 (2022): 405–12. http://dx.doi.org/10.35516/jjps.v15i3.416.

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Streptomyces species are the most prolific producers of antibiotics within the group actinobacteria. The in-vitro antimicrobial and antioxidant activities of the methanol (MeOH) extract and vacuum liquid chromatography (VLC) fractions of a soil bacteria Streptomyces canus ATCC 12647 were evaluated. Agar well diffusion method was used for the antimicrobial assay, while phosphomolybdate and DPPH radical scavenging methods were used for the antioxidant assay. The antimicrobial assay showed remarkable activities against Bacillus subtilis, Staphylococcus aureus, and Candida albicans. Also, the extr

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Holder, I. A., and S. T. Boyce. "Agar well diffusion assay testing of bacterial susceptibility to various antimicrobials in concentrations non-toxic for human cells in culture." Burns 20, no. 5 (1994): 426–29. http://dx.doi.org/10.1016/0305-4179(94)90035-3.

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Malhotra, S.P.K. and Mandal T.K. "Phytochemical screening and in vitro antibacterial activity of Moringa oleifera (Lam.) leaf extract." Archives of Agriculture and Environmental Science 3, no. 4 (2018): 367–72. https://doi.org/10.26832/24566632.2018.030406.

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The aim of the study was to investigate the phytochemical constituents and antibacterial activity of ethanolic extract of <em>Moringa oleifera </em>Lam. belonging to family Moringaceae. Distilled water and ethanol was used to extract the bioactive compounds from the leaves of <em>M. oleifera </em>to detect the phytochemical constituents and to screen its antibacterial activity. The phytochemical constituents were screened by qualitative analysis method. The phytochemical screening indicated the presence of tannins, flavonoids, glycosides, terpenoids, phenols, etc., in leaf

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Sujatha, Soja, and Sunilkumar T. Pillai. "Phytochemical, Antimicrobial and Antioxidant studies on the leaf extract of an endemic medicinal plant Canthiumera neilgherrensis(Wight) K.M.Wong (Rubiaceae)." INTERNATIONAL JOURNAL OF PLANT AND ENVIRONMENT 9, no. 01 (2023): 32–38. http://dx.doi.org/10.18811/ijpen.v9i01.05.

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Canthiumera neilgherrensis (Wight) K.M. Wong is a rare medicinal plant that is endemic to Southern Western Ghats. Qualitative analysis of phytoconstituents, estimation of total phenol, flavonoids, terpenoids, alkaloids, and steroids, Antioxidant activity is determined by FRAP and DPPH assay, Antibacterial analysis by agar well gel diffusion method wasdone. Phytochemicals are mostly concentrated in chloroform and methanol extracts. The highest inhibition was found in water extract against Pseudomonas aeruginosa and which is a gram-negative bacterium. From the result of the assays, it was clear

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Feridoz, Jannathul, and Anitha Roy. "ANTIBACTERIAL ACTIVITY OF AQUEOUS ALCOHOLIC EXTRACT OF ABUTILON INDICUM AERIAL PARTS AGAINST E .FAECALIS – AN INVITRO STUDY." Asian Journal of Pharmaceutical and Clinical Research 10, no. 5 (2017): 80. http://dx.doi.org/10.22159/ajpcr.2017.v10i5.15636.

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Objective: To evaluate the antibacterial activity of the aqueous alcoholic extracts of Abutilon indicum aerial parts against Enterococcus faecalis.Methods: Agar well diffusion assay, as well as microbroth dilution assays, were used for determination of antibacterial activity. The assay was done intriplicate, and chlorhexidine 0.2% was used as the control.Results: Different concentrations (200, 400, and 800 mg/mL) of the ethyl acetate extract of A. indicum aerial parts showed dose-dependantantibacterial activity. The maximum zone of inhibition was 30 mm at the maximum concentration used (800 mg

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Suryawanshi, Pooja, and G. M. Vidyasagar. "Antimicrobial Activity of Opuntia cochenillifera (L.) Mill Fruit and Cladode Extracts." International Journal of Pharmacology, Phytochemistry and Ethnomedicine 3 (June 2016): 84–89. http://dx.doi.org/10.18052/www.scipress.com/ijppe.3.84.

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In the present study the antimicrobial activity of chloroform and methanolic extracts of Opuntiacochenillifera for both cladode and fruits was investigated. Methanolic extract was found to be an effective against the microbes namely, E. coli,Bacillus subtilis,Staphylococcus aureus, Pseudomonas aeruginosaCandidaalbicanC.glabrataC.haemuloniiC.Tropicalis. Maximum activity was observed against E. coli, B. Subtilisand,C.albican and C.glabrata at 40mg/ml. Agar well diffusion assay was used to determine minimum inhibitory concentration of all test microorganisms.

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Mussi, Jamili Maria Suhet, Guilherme Campos Tavares, Ethiene Luiza de Souza Santos, et al. "Antagonistic activity of lactic acid bacteria isolated from Minas artisanal cheeses against Brucella abortus." Brazilian Journal of Veterinary Research and Animal Science 60 (August 21, 2023): e204539. http://dx.doi.org/10.11606/issn.1678-4456.bjvras.2023.204539.

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This study aimed to evaluate methods for studying the in vitro antimicrobial activity of lactic acid bacteria (LAB) against Brucella abortus and to evaluate the antagonistic effect of LAB on the viability of this pathogen. A total of 18 LAB strains (Lactobacillus plantarum, n = 11; Pediococcus acidilactici, n = 1; Lactobacillus rhamnosus, n = 4; and Lactobacillus brevis, n = 2), isolated from Minas artisanal cheeses produced in three regions (Canastra, Campos das Vertentes, and Araxá) of Minas Gerais State, Brazil, were tested for their antimicrobial activity against B. abortus using three met

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Nazia Gulzar, Nazia Gulzar, Saiqa Andleeb Saiqa Andleeb, Shaukat Ali Shaukat Ali, Sadia Nazer Sadia Nazer, and Tariq Iqbal Muhammad Abdul Rauf Khan and Abida Raza Tariq Iqbal Muhammad Abdul Rauf Khan and Abida Raza. "Screening of Antibacterial, Anti-Biofilm, Cell Proliferation Inhibition, and Synergistic Effects of Biogenic Synthesized Silver Nanostructures Using Trillium govanianum with Antibiotics." Journal of the chemical society of pakistan 42, no. 1 (2020): 120. http://dx.doi.org/10.52568/000616.

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The aim of study is to develop the economic plant-based antibacterial agents to improve the treatment strategies for bacterial infections at nano-scale. In current research silver nanostructures synthesis was carried out using Trillium govanianum aqueous extract. Characterizations were done using UV–Visible spectrophotometer, scanning electron microscopy, X-ray diffraction and Fourier transform infrared spectroscopy. Antibacterial efficacy of green-synthesized silver nanostructures and aqueous extract was evaluated via agar well diffusion method, crystal violet assay (antibiofilm analysis), te

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Nazia Gulzar, Nazia Gulzar, Saiqa Andleeb Saiqa Andleeb, Shaukat Ali Shaukat Ali, Sadia Nazer Sadia Nazer, and Tariq Iqbal Muhammad Abdul Rauf Khan and Abida Raza Tariq Iqbal Muhammad Abdul Rauf Khan and Abida Raza. "Screening of Antibacterial, Anti-Biofilm, Cell Proliferation Inhibition, and Synergistic Effects of Biogenic Synthesized Silver Nanostructures Using Trillium govanianum with Antibiotics." Journal of the chemical society of pakistan 42, no. 1 (2020): 120. http://dx.doi.org/10.52568/000616/jcsp/42.01.2020.

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The aim of study is to develop the economic plant-based antibacterial agents to improve the treatment strategies for bacterial infections at nano-scale. In current research silver nanostructures synthesis was carried out using Trillium govanianum aqueous extract. Characterizations were done using UV–Visible spectrophotometer, scanning electron microscopy, X-ray diffraction and Fourier transform infrared spectroscopy. Antibacterial efficacy of green-synthesized silver nanostructures and aqueous extract was evaluated via agar well diffusion method, crystal violet assay (antibiofilm analysis), te

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Ravi, Lokesh, Manasvi V, and Praveena Lakshmi B. "ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF SAPONIN FROM ABUTILON INDICUM LEAVES." Asian Journal of Pharmaceutical and Clinical Research 9, no. 9 (2016): 344. http://dx.doi.org/10.22159/ajpcr.2016.v9s3.15064.

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ABSTRACTObjective: Aim of this study is to analyze the antibacterial and antioxidant potential of crude saponin extract (CSE) from Abutilon indicum leaves.Methods: CSE was subjected for gas chromatography-mass spectrometry (GC-MS) analysis to identify its components. Antibacterial potentialwas analyzed using agar well diffusion method and minimum inhibitory concentration (MIC) was detected using 96-well plate method, againstStaphylococcus aureus (MTCC: 3160) and Escherichia coli (MTCC: 443). DNA damage study was performed using comet assay. Antioxidant capabilitywas studied using 2,2-diphenyl-

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Nofiani, Risa, Rizky Rizky, and Ridho Brilliantoro. "Antibacterial Activities and Toxicity of Streptosporangium sp. SM1P." Molekul 16, no. 3 (2021): 210. http://dx.doi.org/10.20884/1.jm.2021.16.3.780.

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This study aims to explore the anti-bacterial and toxicity activities from a rare actinobacterium isolated from mangrove, Mempawah District, West Kalimantan. The mangrove mud sample from Mempawah district was inoculated on ISP4 agar using a pour plate method. After 4 days of incubation, a colony of suspected actinobacterium was appeared, then isolated and coded as SM1P. SM1P was characterized based on morphological and biochemical traits and identified as a genus of Streptroporangium then called Streptroporangium sp. SM1P. Streptroporangium sp. SM1P was carried out anti-bacterial assay on both

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Swathi, B. G., B. S. Smruthi, B. Saima, and T. R. Prashith Kekuda. "Insecticidal, antimicrobial and antioxidant activity and elemental analysis of Cochlospermum religiosum (L.) Alston (Bixaceae)." Journal of Drug Delivery and Therapeutics 9, no. 2-s (2019): 422–28. http://dx.doi.org/10.22270/jddt.v9i2-s.2551.

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Objectives: Cochlospermum religiosum (L.) Alston is one of the extensively used medicinal plant belonging to the family Bixaceae. In the present study, we determined antimicrobial, antioxidant, and insecticidal activity and elemental analysis of C. religiosum flowers. Methods: Shade dried and powdered flower material was extracted by maceration process using methanol. Antibacterial activity of flower extract was determined by agar well diffusion assay against gram positive and gram negative bacteria. Antifungal activity was tested against two molds namely Rhizopus sp. and Curvularia sp. by poi

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Ahmed, Md Sohel, Israt Jahan Khan, Shahbaz Aman, et al. "Phytochemical investigations, in-vitro antioxidant, antimicrobial potential, and in-silico computational docking analysis of Euphorbia milii Des Moul." Journal of Experimental Biology and Agricultural Sciences 11, no. 2 (2023): 380–93. http://dx.doi.org/10.18006/2023.11(2).380.393.

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Euphorbia milii Des Moul is a deciduous bush indigenous to Madagascar. The present study aims to investigate the presence of the phytochemical, in-vitro antioxidant and antimicrobial potency, and in-silico computational analysis of ethanolic and aqueous preparations of E. milii leaves and flowers. The ethanolic and aqueous extracts were tested for in-vitro antioxidant activity by DPPH, H2O2, TAC, and FRAP assay. In addition, antimicrobial potentials were assayed by agar well diffusion technique against Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, and

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Oleghe, Peace Omoikhudu, Kin Agholor, Lucy Fatima Olusola, Idris Abubakar, and Victory Ayomikun Orhewere. "Correlative antimicrobial assay of various foreign toothpaste against oral microbial isolates." World Journal of Advanced Research and Reviews 7, no. 1 (2020): 245–52. https://doi.org/10.5281/zenodo.4316980.

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The <em>in-vitro</em> antimicrobial assay of four (4) foreign toothpastes sold in Nigeria viz; Sensodyne<sup>®</sup>, Raiya<sup>®</sup>, Mentadent<sup>®</sup> and Colgate<sup>® </sup>were carried out using agar well diffusion method against eight (8) oral clinical isolates which include the strains of  <em>Streptococcus mutans</em>, <em>Brahamella catarrhalis</em>, <em>Veillonella </em>spp, <em>Micrococcus luteus</em>, <em>Lactobacillus </em>spp, <em>Staphylococcus aureus</em>, <em>Streptococcus pneumoniae</e

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Mustafa, Rozina Ghulam, Andleeb Dr Saiqa, Jorge Domínguez, et al. "Therapeutic Values of Earthworm Species Extract from Azad Kashmir as Anticoagulant, Antibacterial, and Antioxidant Agents." Canadian Journal of Infectious Diseases and Medical Microbiology 2022 (February 7, 2022): 1–20. http://dx.doi.org/10.1155/2022/6949117.

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Aims. Current research aimed to explore the therapeutic values of different earthworms as antibacterial, anticoagulant, and antioxidant agents. Methods. Ten different earthworms, i.e., Amynthas corticis, Amynthas gracilis, Pheretima posthuma, Eisenia fetida, Aporrectodea rosea, Allolobophora chlorotica, Aporrectodea trapezoides, Polypheretima elongata, Aporrectodea caliginosa, and Pheretima hawayana, were collected and screened for biological activities. Antibacterial effect analysis of earthworm species was done against fourteen bacterial pathogens, i.e., Escherichia coli, Serratia marcescens

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Bonita S, Elivilia Oktaviana. "ANTIBACERIAL ACTIVITY OF ENDOPHYTIC FUNGI ISOLATE OF PAINTED NETTLE (Coleus scutellarioides L. Benth.) AGAINST SKIN INFECTION BACTERIA USING TLC-BIOAUTOGRAPH AND AGAR DIFFUSION METHODS." Journal Microbiology Science 2, no. 2 (2022): 27–37. http://dx.doi.org/10.56711/jms.v2i2.859.

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Painted Nettle (Coleus scutellarioides L. Benth.) has properties as anti-inflammatory, antioxidant, and antibacterial. It accelerates wound healing as well. Painted nettle contains essential oils, phenols, tannins, phytosterols, alkaloids, and flavonoids. This study aimed to obtain endophytic fungi isolates that have the potential as antibacterial by TLC-Bioautography and agar diffusion methods. This research was conducted by isolating endophytic fungi of painted nettle, advanced to purification and macroscopic tests and nine pure isolates were obtained. The results of the isolate screening wi

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Kumar M, Santosh. "Exploring the therapeutic properties of a resilient marine gastropod Planaxis sulcatus (Born, 1778) of Karwar coast, India." African Journal of Biological Sciences 6, no. 5 (2024): 4623–39. http://dx.doi.org/10.48047/afjbs.6.5.2024.4623-4639.

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The Marine environment is a pivotal resource for bioprospecting therapeutically active natural compounds. Molluscs are known to harbour compounds with antimicrobial, antitumor and antioxidant activities. The aim of current investigation was to evaluate the antimicrobial, anticancer, and antioxidant activities of the crude whole body methanol extract of Planaxis sulcatus. FTIR was employed to identify the types of chemical bonds and functional groups present in the extract. Antimicrobial activity was assessed using Agar well diffusion assay, and determination of MIC, MBC and MFC of the extract

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Martins, Yugo Araújo, Reginaldo dos Santos Sousa, and Cristiani Lopes Capistrano Gonçalves de Oliveira. "Development and Validation of a Microbiological Agar Assay for Determination of Thiamphenicol in Soft Capsules." Current Pharmaceutical Analysis 16, no. 7 (2020): 806–13. http://dx.doi.org/10.2174/1573412915666190328213828.

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Background: Thiamphenicol belongs to the amphenicol class of antibiotic and possesses a broad-spectrum antimicrobial activity. An alternative microbiological assay for quantification of thiamphenicol in pharmaceutical formulations has not yet been reported in the literature. Objective: This study aimed to develop and validate an agar diffusion method for quantification of thiamphenicol in soft capsules. Methods: The assay was based on the inhibitory effect of thiamphenicol on the following: a strain of Kocuria rhizophila ATCC 9341, used as the test microorganism, Antibiotic 1culture medium, ph

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Iftikhar, Noreen, and Iqra Khalid. "Isolation and Characterization of Antibiotic-producing Bacteria from the Salt Range of Kallar Kahar, Pakistan." TSF Journal of Biology 1, no. 1 (2023): 67–75. http://dx.doi.org/10.69547/tsfjb.v1i1.10.

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The emergence of antibiotic resistance in pathogenic bacteria has heightened the need for new antibiotics. Streptomycetes are filamentous gram-positive bacteria that are ubiquitous and present in saline soil and produce antibiotics as secondary metabolites under stressful conditions. Naturally, Streptomyces produce over two-thirds of antibiotics that are used clinically. Saline soil was collected from Kallar Kahar, Pakistan. The soil sample was serially diluted and three dilutions were plated on Streptomyces selection media (starch-casein agar and glucose yeast malt agar) after growing the cul

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Sajid, Numan, Shehla Khalid, Abid Mahmood, et al. "Synthesis of N-([1,1'-biphenyl]-4-ylmethyl)-4- methylpyridin-2-amines and Antibacterial Evaluation: Agar Well Diffusion Method, Docking Studies and DFT Insights." Chiang Mai Journal of Science 52, no. 3 (2025): 1–14. https://doi.org/10.12982/cmjs.2025.023.

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The pharmaceutical sector is facing major concerns in addressing effective drug molecules against extensively drug-resistant S. Typhi. Exploiting the medicinal benefits of amines, we synthesized novel small organic molecules bearing both pyridine and secondary amine linkages. The N-(4-bromobenzyl)-4-methylpyridin-2-amine (3) and derivatives (5a–c) have been synthesized which are effective against multiple drugresistant S. Typhi. Then, the antibacterial efficacy of these compounds was determined by using Agar Well diffusion assay (AWDA). Compound 5b was active (MIC 3.125 mg/mL). Molecular docki

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HSIEH, HSING-YI, HYUN-DONG PAIK, and BONITA A. GLATZ. "Improvement of Detection and Production of Propionicin PLG-1, a Bacteriocin Produced by Propionibacterium thoenii†." Journal of Food Protection 59, no. 7 (1996): 734–38. http://dx.doi.org/10.4315/0362-028x-59.7.734.

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Propionibacterium thoenii P127 produces propionicin PLG-1 in liquid culture at relatively low concentrations and slow production rates. The goal of this study was to increase the sensitivity and reproducibility of the standard well-diffusion assay for bacteriocin activity as well as to improve production of propionicin PLG-1 under controlled conditions in a fermenter. Several conditions were varied in the well-diffusion assay. The best results were obtained with 7-mm wells cut into a 5-mm-deep base layer that contained 2.5% agar, 0.85% NaCl and 0.1 % Tween 80. Plates were incubated at room tem

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Shahedan, Puteri Nadia, Elysha Nur Ismail, and Reezal Ishak. "A Study on the Efficacy of Alcohol Based and Alcohol Free Hand Sanitizers Available in the Market Against Staphylococcus Aureus and Salmonella Typhimurium." ICMST 19, s9 (2023): 333–36. http://dx.doi.org/10.47836/mjmhs.19.s9.45.

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Hand sanitizers can be manufactured in the form of alcohol based hand sanitizer (ABHS) that contains alcohol, or alcohol free hand sanitizer (AFHS) containing benzalkonium chloride. This study determines the efficacy of ABHS and AFHS products against Staphylococcus aureus and Salmonella Typhimurium. Agar well diffusion assay was performed against the bacterial colonies grown on Mueller Hinton agar in triplicates. Wells were made by punching holes onto the agar and filled with three different brands of hand sanitizers namely 70% ABHS, 95% ABHS, and AFHS. The diameters for zone of inhibition was

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Ali, Zainab. "Screening of marcescin producing isolates from Serratia marcescens." Academic Science Journal 2, no. 4 (2024): 207–16. http://dx.doi.org/10.24237/asj.02.04.811d.

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The current study intends to investigate the production of bacteriocin from S. marcescens, a Gram-negative bacterium, is a human pathogen and the reason why hospital medical equipment becomes contaminated. Various samples from patients about 220 samples which include blood,urine,abscess and sputum, twenty -three isolates of S.marcescens was performed as per standard laboratory procedure and confirmed by using VITEK-2 systems. The isolates of S.marcescens which produced marcescin detected by four methods : spot –on-the –lawn method , Cup-assay method ,Well-diffusion method and Agar cross streak

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Nawarathne, Nirmani Wishwakala, Kanchana Wijesekera, Gaya Bandara Wijayaratne, and Mayuri Napagoda. "Development of novel topical cosmeceutical formulations with antimicrobial activity against acne-causing microorganisms from <em>Coriandrum sativum</em> L." Journal of Science 14, no. 1 (2023): 1–17. http://dx.doi.org/10.4038/jsc.v14i1.60.

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Spices possess a diverse array of natural phytochemicals with antibacterial, anti-inflammatory, and antioxidant effects. Hence spices could be employed to treat chronic dermatologic conditions like acne vulgaris which involves infection of Propionibacterium acnes and Staphylococcus aureus, and inflammation. Particularly with the emergence of antibiotic resistance, there is an utmost necessity for the development of novel therapeutic agents for the management of acne. Thus, this study was focused on the development of novel topical gel formulations from the seeds of Coriandrum sativum L. (coria

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Sadaf, Huma Mehreen, Yamin Bibi, Muhammad Ishaque, et al. "Determination of ROS Scavenging, Antibacterial and Antifungal Potential of Methanolic Extract of Otostegia limbata (Benth.) Boiss." Plants 10, no. 11 (2021): 2360. http://dx.doi.org/10.3390/plants10112360.

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Wide spectrum medicinal significance augments plant utilization as the primary source of significant pharmaceutical agents. In vitro investigation of antioxidant and antimicrobial activity highlights the therapeutic potential of Otostegia limbata. Methanol extract of the plant (MEP) shows considerable dose dependent antioxidant ability at six concentrations (7.81 µg/mL to 250 µg/mL) in 2.2-diphenyl-1-picrylhydrazyl (DPPH) assay, phosphomolybdate assay (PMA) and reducing power assay (RPA). The plant capability to scavenge free radicals in the mixture ranged from 37.89% to 63.50% in a concentrat

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