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1

Shukla, S., H. K. Yadav, A. Rastogi, B. K. Mishra, and S. P. Singh. "Alkaloid diversity in relation to breeding for specifi c alkaloids in opium poppy (Papaver somniferum L.)." Czech Journal of Genetics and Plant Breeding 46, No. 4 (2010): 164–69. http://dx.doi.org/10.17221/96/2009-cjgpb.

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Papaver somniferum is a chief source of diverse physiologically active alkaloids, required by the pharmaceutical industry. The present study describes the diversity of the alkaloid spectrum of 122 opium poppy accessions of Indian origin by means of a cluster analysis based on Mahalanobis generalised distances. The accessions could be grouped into 11 clusters according to their relationship between the contents of morphine, codeine, thebaine, narcotine and papaverine in raw opium. The diversity of the alkaloid spectrum of 11 clusters reflected the very low correlations between the contents of t
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2

Sun, Bo, Yu-Xiao Tian, Fen Zhang, et al. "Variations of Alkaloid Accumulation and Gene Transcription in Nicotiana tabacum." Biomolecules 8, no. 4 (2018): 114. http://dx.doi.org/10.3390/biom8040114.

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To increase the understanding of alkaloid biosynthesis in Nicotiana tabacum during whole plant growth periods, variations of the contents of alkaloids and the transcription of key biosynthetic genes in fresh leaves were investigated in three varieties at five developmental stages. Six alkaloids were analyzed by gas chromatograph–mass spectrometry (GC–MS) and the most abundant alkaloid was observed during the upper leaves maturing stage in the varieties, among which the alkaloid content of K326 was the highest. Considering the genetic effect, variance analysis indicated that the developmental s
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3

Chizzola, Remigius. "Pyrrolizidine Alkaloids in Adenostyles alliariae and A. glabra from the Austrian Alps." Natural Product Communications 10, no. 7 (2015): 1934578X1501000. http://dx.doi.org/10.1177/1934578x1501000710.

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The alkaloid content of Adenostyles alliariae and A. glabra (Asteraceae) has been evaluated. Both species contain toxic macrocyclic unsaturated pyrrolizidine alkaloids with seneciphylline as the main compound accounting for more than 90% of the alkaloid fraction in all above ground plant parts. Further alkaloids were spartioidine, acetyl-senciphylline and senecionine. Inflorescences showed the highest alkaloid contents with 21.1 and 13.4 mg/g in A. alliariae and A. glabra, respectively. Stems and leaves had 2–3 times lower contents. Therefore, these Adenostyles species must be considered as hi
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4

Liu, Jing, Kaifeng Shi, Jia Shi, et al. "A simple strategy to monitor the temporal and spatial distribution of alkaloids in sacred lotus leaves." Bioscience, Biotechnology, and Biochemistry 85, no. 6 (2021): 1332–40. http://dx.doi.org/10.1093/bbb/zbab038.

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ABSTRACT Owing to the high degree of diversity of metabolite pools and complexity of spatial and temporal distributions within biological tissues, currently available methods for metabolite characterization face large challenges. In this study, the temporal and spatial distributions of the alkaloid components of the medicinal plant lotus (Nelumbo nucifera) were investigated over various growth phases. The results showed that alkaloid biosynthesis in lotus leaf is regulated by development and that there is maximum accumulation of alkaloids when the lotus leaf was completely expanded. Furthermor
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5

Staples, Kristina D., Anwar A. Hamama, Regina Knight-Mason, and Harbans L. Bhardwaj. "Alkaloids in White Lupin and Their Effects on Symbiotic N Fixation." Journal of Agricultural Science 9, no. 6 (2017): 13. http://dx.doi.org/10.5539/jas.v9n6p13.

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Seeds of white lupin (Lupinus albus L.), known to potentially fix 150 to 200 kg/ha N via Symbiotic N Fixation (SNF), are classified as sweet or bitter based on their alkaloid contents: sweet lupins contain very low whereas bitter lupins contain high contents of alkaloids. However, precise information about effects of alkaloid content on SNF is not very well unknown. Experiments were conducted to determine if alkaloid content in the seed is related to that in other plant tissue such as leaves and pod shells, characterization of relationship between alkaloid concentration and SNF, and to study v
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6

Salma, Tarannum. "SPECTROPHOTOMETRIC ESTIMATION OF TOTAL ALKALOIDS IN CHITRAKA (PLUMBAGO ZEYLANICA LINN) USING BROMOCRESOL GREEN." Avishkara 01, no. 01 (2022): 03–05. http://dx.doi.org/10.56804/avishkara.2022.1102.

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Background: Plumbago zeylanica L. (Plumbaginaceae) commonly known, as Chitraka is pharmacologically important plant. Various studies have been undertaken to assess the pharmacological potential of different parts of the plant namely like roots, stem, flower, and leaves as antimicrobial, hepatoprotective, anticancer, antifertility, antiulcer, antifungal and wound healing. Chitraka is rich in alkaloids hence present study is taken. Objective: The objective of the present study was to determine the total alkaloid content in Chitraka Materials & Methods: The presence of alkaloids was confirmed
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7

Sari, Eni Kartika, Siti Maimunah, and Mega Karina Putri. "THE EFFECT OF MACERATION TIME ON TOTAL ALKALOID LEVELS IN BROCOLI (Brassica oleracea var. italica) BY USING UV-Vis SPECTROPHOTOMETRY METHOD." Jurnal Jamu Kusuma 2, no. 1 (2022): 38–46. http://dx.doi.org/10.37341/jurnaljamukusuma.v2i1.29.

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ABSTRACT
 Broccoli is known to be very rich in secondary metabolites, such as alkaloids, flavonoids, tannins, polyphenols, essential oils, and polypeptides. Alkaloids are one of the secondary metabolites that are commonly found in nature and have physiological activities. The extraction time factor is the thing that can affect the quality of the extraction results which in this study is the alkaloid content. This study aims to determine the optimal maceration time so that it can produce optimum total alkaloid content. The stages in this study include sample preparation and then sample ext
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8

Germán-Soto, Brianda Giselle, José Basilio Heredia, Nayely Leyva-López, et al. "Bioaccessibility and Antioxidant Capacity of Alkaloids from Microencapsulated Extract of Eggplant (Solanum melongena L.) Biomass." Horticulturae 10, no. 12 (2024): 1242. http://dx.doi.org/10.3390/horticulturae10121242.

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Eggplant is a vegetable grown worldwide, and due to quality standards, large amounts of biomass are generated after harvest. Biomass is considered a source of bioactive compounds with antioxidant properties. Therefore, this research aimed to evaluate the bioaccessibility (BA) and antioxidant capacity of microencapsulated alkaloids from eggplant fruit biomass. Eggplant biomass was collected, and the total alkaloid content, antioxidant capacity (TEAC, FRAP, and ORAC), and alkaloid profile (UPLC/MS) were determined before and after the in vitro digestion of encapsulated and non-encapsulated alkal
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9

Májer, Péter, and Éva Zámboriné Németh. "Alkaloid Accumulation and Distribution within the Capsules of Two Opium Poppy (Papaver somniferum L.) Varieties." Plants 13, no. 12 (2024): 1640. http://dx.doi.org/10.3390/plants13121640.

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The goal of this study was to clarify the role of capsule size and morphology in the alkaloid yield of poppy. In 2023, two industrial varieties from large-scale cultivation were investigated. Three classes of capsule size (large, medium, and small) and four organelles (wall, placenta, disc, and thalamus) of the seedless capsule were studied for their mass proportions and alkaloid contents. In ‘Meara’, large capsules had 41% lower total alkaloid levels than smaller ones. In ‘Morgana’, there was no difference in total alkaloids between size groups, but large capsules had higher contents of codei
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10

Galek, R., E. Sawicka-Sienkiewicz, D. Zalewski, S. Stawiński, and K. Spychała. "Searching for low alkaloid forms in the Andean lupin (Lupinus mutabilis) collection." Czech Journal of Genetics and Plant Breeding 53, No. 2 (2017): 55–62. http://dx.doi.org/10.17221/71/2016-cjgpb.

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The Andean lupin (Lupinus mutabilis Sweet) is the only cultivated species of the genus Lupinus originating from South America. Attempts were made to introduce this species to European soil and climatic conditions. The main disadvantages of the Andean lupin include too long and non-uniform maturation of pods in a plant, dropping flowers and pod buds, and a high alkaloid content (up to 5%). The aim of this study was to identify in post-mutagen treated material genotypes with low alkaloid content in seeds, which would be useful for improving L. mutabilis by breeding. The iodine test was annually
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11

Weber, Holly A., Matthew K. Zart, Andrew E. Hodges, et al. "Method Validation for Determination of Alkaloid Content in Goldenseal Root Powder." Journal of AOAC INTERNATIONAL 86, no. 3 (2003): 476–83. http://dx.doi.org/10.1093/jaoac/86.3.476.

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Abstract A fast, practical ambient extraction methodology followed by isocratic liquid chromatography (LC) analysis with UV detection was validated for the determination of berberine, hydrastine, and canadine in goldenseal (Hydrastis canadensis L.) root powder. The method was also validated for palmatine, a major alkaloid present in the possible bioadulterants Coptis, Oregon grape root, and bar-berry bark. Alkaloid standard solutions were linear over the evaluated concentration ranges. The analytical method was linear for alkaloid extraction using 0.3–2 g goldenseal root powder/100 mL extracti
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12

Setyaningrum, Lindawati, and Dhina Ayu Susanti. "PENETAPAN KADAR ALKALOID TOTAL PADA EKSTRAK N-HEKSAN DAN ETANOL BIJI KETUMBAR (Coriandrum sativum) MENGGUNAKAN SPEKTROFOTOMETER UV-Vis." Jurnal Riset Kefarmasian Indonesia 4, no. 3 (2022): 353–65. http://dx.doi.org/10.33759/jrki.v4i3.268.

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Coriander (Coriandrum sativum) is one of the medicinal plants which is known to contain secondary metabolites such as flavonoids, tannins, terpenoids, saponins, steroids, and alkaloids. Alkaloids have therapeutic effects as antimalarials and cancer. Research in determining the alkaloids in coriander was carried out by separating the alkaloids from other substances using the soxhlet method extraction with two solvents ethanol and n-hexane. The total alkaloid content is determined using a UV-VIS spectrophotometer at wavelength 351,5 nm. The reaction of alkaloids with Bromocresol green (BCG) is o
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13

Święcicki, Wojciech, Katarzyna Czepiel, Paulina Wilczura, Paweł Barzyk, Zygmunt Kaczmarek, and Magdalena Kroc. "Chromatographic Fingerprinting of the Old World Lupins Seed Alkaloids: A Supplemental Tool in Species Discrimination." Plants 8, no. 12 (2019): 548. http://dx.doi.org/10.3390/plants8120548.

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The total contents and qualitative compositions of alkaloids in seeds of 10 Old World lupin species (73 accessions) were surveyed using gas chromatography. The obtained results, combined with those for three lupin crops, Lupinus angustifolius, Lupinus albus, and Lupinus luteus, provide the most complete and up-to-date overview of alkaloid profiles of 13 lupin species originating from the Mediterranean Basin. The qualitative alkaloid compositions served as useful supplementary tools of species discrimination. On the basis of the most abundant major alkaloids, lupanine, lupinine, and multiflorin
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14

Yue, Hong Kun, Ming Chang, Si Jie Liu, and Ran Zhou. "Determination of Content of the Four Alkaloids and the Total Alkaloid in Co-Dergocrine Mesyiate Tablets by HPLC." Advanced Materials Research 884-885 (January 2014): 419–22. http://dx.doi.org/10.4028/www.scientific.net/amr.884-885.419.

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Objective: To determine the four kinds of alkaloid and the total alkaloid in Co-dergocrine Mesyiate tablets by HPLC. Methods: HPLC was used. C18column (250mm×4.6mm5μm) was selected, the mobile phase was acetonitrile-H2O-triethylamine (50:150:5).Detection was performed by UV with wavelength of 280nm.Results:A linearity of alkaloid I was obtained from 240.336 to 961.344μg/mL with good correlation (r=0.9997). The average recovery of alkaloid Iwas 101.16%, and RSD was0.92%. A linearity of alkaloidII(α isomer) was obtained from 240.336 to 961.344μg/mL with good correlation (r=0.9998). The average r
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15

Vishnyakova, M. A., A. V. Salikova, T. V. Shelenga, G. P. Egorova, and L. Yu Novikova. "Alkaloid content variability in the seeds of narrow-leafed lupine accessions from the VIR collection under the conditions of the Russian Northwest." Vavilov Journal of Genetics and Breeding 27, no. 2 (2023): 119–28. http://dx.doi.org/10.18699/vjgb-23-17.

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Alkaloid content was assessed in the seeds of 59 narrow-leafed lupine (Lupinus angustifolius L.) accessions from the VIR collection in the environments of Leningrad Province. The selected set included accessions of different statuses (wild forms, landraces, and advanced cultivars) and different years of introduction to the collection. Alkaloids were analyzed using gas-liquid chromatography coupled with mass spectrometry. Concentrations of main alkaloids: lupanine, 13-hydroxylupanine, sparteine, angustifoline and isolupanine, and their total content were measured. The total alkaloid content var
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16

Slavík, Jiří, and Leonora Slavíková. "Alkaloids from Papaver albiflorum PACZ. subsp. albiflorum and P. cf. stevenianum A. D. Mikheev." Collection of Czechoslovak Chemical Communications 55, no. 7 (1990): 1812–16. http://dx.doi.org/10.1135/cccc19901812.

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Papaver albiflorum PACZ. subsp. albiflorum (tetraploid, 2n = 28; total alkaloid content 0.001%) afforded corytuberine, protopine and allocryptopine as dominant alkaloids; in addition, small amounts of macambrine, thebaine, scoulerine, rhoeadine, papaverrubines D, C, A and E, berberine and coptisine were detected. Papaver cf. stevenianum A. D. MIKHEEV (hexaploid, 2n = 42; total alkaloid content 0.066% and 0.015%, respectively) gave berberine as the principal alkaloid (0.065% and 0.013, respectively) which was responsible for the yellow colour of latex. Corytuberine, allocryptopine, protopine an
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17

Haddad, J., M. Muzquiz, and K. Allaf. "Treatment of Lupin Seed Using the Instantaneous Controlled Pressure Drop Technology to Reduce Alkaloid Content." Food Science and Technology International 12, no. 5 (2006): 365–70. http://dx.doi.org/10.1177/1082013206070160.

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Despite their high protein content, lupin seeds are not as fully utilised as some other grain legumes. The drawback in their utilisation is mainly due to the presence of water soluble, poisonous alkaloids. In this study, an instantaneous controlled pressure drop (DIC) treatment followed by an aqueous extraction was performed on lupin seeds. Two species Lupinus albus and Lupinus mutabilis with different initial total alkaloid content of 0.025% and 5.5% (d.b.), respectively have been studied. Lupanine was the major alkaloid found in both lupin species. Experimental results proved the feasibility
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18

Hakim, Ali Rakhman, Putri Olivia Nayaken, and Tuti Alawiyah. "Pengaruh Metode Ekstraksi terhadap Kadar Alkaloid Total Ekstrak Daun Kirinyuh (Chromolaena odorata)." Indonesian Journal of Pharmacy and Natural Product 6, no. 02 (2023): 194–200. http://dx.doi.org/10.35473/ijpnp.v6i02.2508.

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Kirinyuh leave (Chromolaena odorata) was used by the community as a potential plant because it contains many benefits, one of which contains alkaloid compounds. Alkaloids have the ability to help wound healing because the active compounds of the alkaloids have antioxidant activity, so the enhanced wound healing by kirinyuh leaf can be caused by the action of free radicals in increasing levels of antioxidant enzymes. The aim of the study was to determine the total alkaloid content of kirinyuh leaf leaf extract in each of the extraction methods to be used. In this study, a different extraction m
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19

Barlóg, P. K. "Effect of magnesium and nitrogenous fertilisers on the growth and alkaloid content in Lupinus angustifolius L." Australian Journal of Agricultural Research 53, no. 6 (2002): 671. http://dx.doi.org/10.1071/ar00111.

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The effect of kieserite (MgSO4.H2O) and nitrogenous fertilisers in various forms [control, Ca(NO3)2, (NH4)2SO4, NH4NO3, CO(NH2)2] on the growth and alkaloid content in L. angustifolius was investigated. Mineral fertilisers were applied at doses of 60 kg/ha of Mg and 75 kg/ha of N before sowing. Kieserite did not have a significant influence on the growth and dry matter production of lupin. Ca(NO3)2 and NH4NO3, although increasing the yield of straw, did not affect the yield of seeds. The application of (NH4)2SO4 decreased the yield of straw and seeds when compared with the effect of the above-
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20

Orlando, B., C. Maumené, and F. Piraux. "Ergot and ergot alkaloids in French cereals: occurrence, pattern and agronomic practices for managing the risk." World Mycotoxin Journal 10, no. 4 (2017): 327–38. http://dx.doi.org/10.3920/wmj2017.2183.

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Ergot is a disease of cereals and grasses caused by the ascomycete fungus Claviceps purpurea (Fr.) Tul. This fungus infects young, usually unfertilised ovaries, replacing the seeds with dark mycelial masses known as sclerotia. The amount of sclerotia in marketable grain is strictly regulated in many countries. We found that ergot and its alkaloids were common in French farm fields during the 2012, 2013 and 2014 harvests. Before cleaning, ergot was present in harvest samples from 23 to 30% of wheat fields, 15 to 30% of durum wheat fields, 27 to 39% of barley fields, 45 to 66% of triticale field
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Tittlemier, S. A., D. Drul, M. Roscoe, and J. G. Menzies. "The effects of selected factors on measured ergot alkaloid content in Claviceps purpurea-infected hexaploid and durum wheat." World Mycotoxin Journal 9, no. 4 (2016): 555–64. http://dx.doi.org/10.3920/wmj2015.2019.

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Four wheat genotypes, including the ergot-susceptible durum ‘AC Avonlea’ and hard red spring wheat ‘AC Cadillac’, as well as the resistant durum wheat line 9260B-173A and the hard red spring wheat line ‘Kenya Farmer’ wereinoculated with different Claviceps purpurea isolates. Honeydew and sclerotia were collected and analysed for 10 ergot alkaloids. Total concentrations of the 10 ergot alkaloids ranged from 16 µg/kg in honeydew to 1,798 mg/kg insclerotia. Ergonovine and ergosine were the predominant alkaloids in honeydew obtained from plants inoculated with various isolates, whereas ergocristin
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Chacón-Fuentes, Manuel, César Burgos-Díaz, Mauricio Opazo-Navarrete, Alan Mercado, and Fernando Westermeyer. "Berberine and Palmatine Distribution Across Plant Organs in Berberis darwinii: Basis for Selecting Superior-Producing Accessions." Molecules 30, no. 8 (2025): 1849. https://doi.org/10.3390/molecules30081849.

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Berberis darwinii, known for its bioactive alkaloids like berberine and palmatine, has gained attention for its medicinal properties. However, comprehensive studies on the specific bioactive molecules of Michay are lacking, as previous research has primarily focused on wild plants. Therefore, this study proposes to evaluate the alkaloid content in various tissues of B. darwinii collected from different locations, aiming to identify high-yielding accessions suitable for consistent bioactive alkaloid production. This research focuses on plants from a cultivated Michay orchard established five ye
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23

Gresser, Gabriele, Ludger Witte, Victor P. Dedkov, and Franz-Christian Czygan. "A Survey of Quinolizidine Alkaloids and Phenylethylamine Tyramine in Cytisus scoparius (Leguminosae) from Different Origins." Zeitschrift für Naturforschung C 51, no. 11-12 (1996): 791–801. http://dx.doi.org/10.1515/znc-1996-11-1205.

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Distribution and content of the quinolizidine alkaloids and phenylethylamine tyramine in various organs of Cytisus scoparius taken from different localities in Germany, Russia, Italy and France were analyzed by capillary GLC. Sparteine and sparteine-derivatives like 11,12- dehydrosparteine are predominant in shoots and flowers whereas lupanine-type alkaloids are mainly accumulated in seeds, pods and roots. During the year no changes within the alkaloid pattern of the young developing shoots could be observed, but the total alkaloid amount increases remarkably at the end of the vegetation perio
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Szabó, Beata, Ágnes Lakatos, Tamás Kõszegi, György Kátay, and L. Botz. "Thin-Layer Chromatography-Densitometry and Liquid Chromatography Analysis of Alkaloids in Leaves of Papaver somniferum Under Stress Conditions." Journal of AOAC INTERNATIONAL 88, no. 5 (2005): 1571–78. http://dx.doi.org/10.1093/jaoac/88.5.1571.

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Abstract The effect of stress conditions on the concentrations of secondary metabolites were examined during various developmental stages of Papaver somniferum plants. P. somniferum plants were grown in laboratory conditions (Budakalász). The experiment consisted of 22 treatments. Significantly different alkaloid contents can be observed under different stress conditions. In general, the alkaloid contents of plants are very low; therefore, a highly sensitive and reliable method has to be developed for analysis. The amount of alkaloids was measured by 2 separation and detection techniques. Accu
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Windarsih, Yulia, Masrianih, and Manap Trianto. "Flavonoid and Alkaloid Content of Red Onion Skin Extract (Allium cepa L.)." Jurnal Biologi Tropis 25, no. 1 (2025): 886–90. https://doi.org/10.29303/jbt.v25i1.8578.

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Red onion skin (Allium cepa L.) is often discarded as waste, despite being rich in flavonoids and alkaloids, which possess antioxidant, anti-inflammatory, antimicrobial, and anticancer properties. This study aims to determine the flavonoid and alkaloid content of red onion skin extract using reflux and Soxhlet extraction methods with 96% ethanol as the solvent. The extraction was performed using reflux and Soxhlet techniques, followed by UV-Vis spectrophotometry to quantify flavonoid and alkaloid content. A quercetin standard curve was used to determine flavonoid levels, while a caffeine stand
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Wilczura, Paulina, Wojciech Święcicki, Katarzyna Kamel, and Wiesław Wasiak. "COLORIMETRIC VS. CHROMATOGRAPHIC ANALYSES OF ALKALOIDS IN LUPIN SEEDS." Plant Breeding and Seed Science 78 (December 20, 2018): 63–67. http://dx.doi.org/10.37317/pbss-2018-0014.

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A characteristic trait of lupins is a production of alkaloids, which are a toxic and bitter taste compound ofseeds. Due to the lack of fast, sensitive and inexpensive screening techniques to identify and reject high alkaloidplant material, development of suitable tools is important challenges for lupins breeding and seed production.The aim of this study was to compare two alkaloid content estimation methods in Lupinus angustifoliusL. and Lupinus albus L.During the Wagner’s colorimetric test, which is recommended by the UPOV, seed halves were stained onfour colors depending on the alkaloid cont
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V., Shirisha* A. Ganga Bhavani A. Varshitha A. Uday Kumar K. Guna Priya K. Satya Deepthi. "Determination And Quantification of Alkaloids Content in Tridax Procumbens Leaves by Using Colorimetric Method." International Journal of Pharmaceutical Sciences 3, no. 3 (2025): 3509–15. https://doi.org/10.5281/zenodo.15115158.

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Tridax procumbens, a widely distributed medicinal plant, is known for its diverse pharmacological properties, including antimicrobial, anti-cancer, anti-oxidant, anti-diabetic, anti-inflammatory, and wound healing effects. The presence of bioactive alkaloids contributes significantly to its therapeutic potential. This study aims to determine and quantify the alkaloid content inTtridax procumbens leaves using a colorimetric method. Alkaloids were extracted using soxhlet extraction techniques, and their concentration was measured colorimetrically. The absorbance was recorded at a specific wavele
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Alfi, Tegar Fernanda. "Determination Of Total Alkaloid Content Of Chinese Betel (Peperomia pellucida) Based On Differences In Ethanol Concentration Using Uv-Vis Spectrophotometry Method." Journal Pharmaceutical Care and Sciences 5, no. 1 (2024): 158–67. https://doi.org/10.33859/jpcs.v5i1.673.

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Background: The Chinese betel plant (Peperomia pellucida) is used by the community as a potential plant because it contains many benefits, one of which contains alkaloid compounds. Alkaloids have the ability to help heal wounds because the active compounds contained in alkaloids have antioxidant activity so that improved wound healing by Chinese betel plants (Peperomia pellucida) can be caused by the action of free radicals in increasing levels of antioxidant enzymes. This research will use the maceration extraction method using 70% ethanol and 96% ethanol solvents which will be carried out us
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Zhapova, Ts, L. D. Modonova, and A. A. Semenov. "Alkaloid content ofAconitum czekanovskyi." Chemistry of Natural Compounds 22, no. 3 (1986): 360. http://dx.doi.org/10.1007/bf00598331.

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Aripova, S. F. "Alkaloid content ofCodonopsis clematidea." Chemistry of Natural Compounds 32, no. 4 (1996): 564–65. http://dx.doi.org/10.1007/bf01372615.

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Bharti, Dipti, Rahul Singh, and Charu Arora. "Study on effect of variation of geographical and climatic conditions on chemical constituents and biological activity of Emblica officinalis." Research Journal of Chemistry and Environment 25, no. 10 (2021): 114–20. http://dx.doi.org/10.25303/2510rjce114120.

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Alkaloid and flavonoid contents of E. officinalis of leaf samples collected from five different geographical and climatic conditions have been compared in the present study. Comparison has also been made in terms of percent yield and number of constituents present in alkaloid and flavonoid samples as well as for antifungal potential against Fusarium oxysporum and Rhizoctonia solani. Alkaloid content is found to be maximum in samples collected from Banasthali, Rajasthan, an extremely hot and dry climatic zone. HPLC profiles indicate presence of maximum number of constituents in alkaloids separa
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32

Muhammad, Zahra B., and Talib O. Al-Khesraji. "A Study of Chemical Content in Some Species of Tribe Apieae / Apiace." Ibn AL- Haitham Journal For Pure and Applied Sciences 2021 (May 28, 2021): 29–37. http://dx.doi.org/10.30526/2021.ihicpas.2647.

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In this study, the flavonoid and alkaloid content in the alcoholic extract of the shoots and flowers were identified in four species of the tribe Apieae / Apiacese : Ammi majus, Ammi visgana, Anethum graveolens and Foeniculum vulgaris, and the flavonoids that were detected are (Apigenin, Coumarin, Kaempferol and Quercetin). The species Foeniculum vulgaris has recorded the highest concentration of total flavonoid content (Shoots and Flowers) among the studied species, reaching 4139.2 µg / ml. The total alkaloids are estimated for these species, and the Foeniculum vulgaris has recorded the high
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33

Popov, V. S., A. V. Salikova, I. N. Perchuk, et al. "Rapid assessment of the main economic value indicators in lupine flour samples using infrared spectroscopy." Proceedings on applied botany, genetics and breeding 185, no. 1 (2024): 99–108. http://dx.doi.org/10.30901/2227-8834-2024-1-99-108.

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Background. A calibration model has been developed for rapid assessment of economic value indicators (protein, oil, and quinolizidine alkaloid contents) in the seeds of narrowleaf lupine accessions from VIR using near-infrared spectroscopy, with the help of which it is possible to decide on the further use of the accessions.Materials and methods. Biochemical quality indicators (protein, oil, and quinolizidine alkaloid content) were studied in the seeds of narrowleaf lupine (Lupinus angustifolius L.) grown in 2019 in the northwest of Russia. Calibration models for measuring protein, oil and alk
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34

Andriani, Cici, Anny Sartika Daulay, Ridwanto Ridwanto, and Haris Munandar Nasution. "Determination of Total Alkaloid Content of Raru (Cotylelobium lanceolatum Craib) Bark Extract Using UV-ViS Spectrophotometry Method." Indonesian Journal of Science and Pharmacy 1, no. 1 (2023): 8–13. https://doi.org/10.63763/ijsp.v1i1.10.

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The raru plant (Cotylelobium lanceolatum Craib) comes from the Dipterocarpaceae family. Raru bark is usually added to the wine to enhance the taste of the wine. raru bark has antidiabetic compounds as a lowering of sugar levels in the blood. The purpose of this study was to determine the alkaloids in the Raru plant (Cotylelobium lanceolatum Craib) and to determine the total alkaloid content found in the Raru plant (Cotylelobium lanceolatum Craib). The extraction method used is the maceration extraction method using methanol solvent with various concentrations which is then evaporated with a ro
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35

Hastuti, Endah Dwi, and Erma Prihastanti. "The effect of inundation levels on secondary metabolites accumulation in Avicennia marina (Forsk.) roots under different salinity regimes." International Journal of Secondary Metabolite 12, no. 2 (2025): 368–80. https://doi.org/10.21448/ijsm.1484387.

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Salinity and inundation are factors that affect secondary metabolites. This research aims to study the range of typical secondary metabolite content in Avicennia marina growing at different salinity levels, analyze the level of inundation that causes peak stress, and examine the impact of inundation stress on A. marina under different salinity regimes. This study used a 2-factor factorial complete randomized design, namely salinity level (15, 20, 25, 30, and 35 ppt), and inundation level (10, 15, and 20 cm). The parameters measured were tannin content, total alkaloids, and total phenols in A.
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36

Dhumal, Jeevan Suresh, Sanjay R. Chaudhari, and Macchindra J. Chavan. "Effect of Different Extraction Conditions on Total Alkaloids, Total Phenolic and Total Flavonoid Content of Vigna mungo L hepper." Journal of Drug Delivery and Therapeutics 9, no. 4 (2019): 89–91. http://dx.doi.org/10.22270/jddt.v9i4.3138.

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Vigna mungo L Hepper is an important pulse crop cultivated in India. It is rich source of many nutritional compounds and it is an integral part of diet. Bioactive compounds like phenolic, alkaloids, flavonoids, saponins etc. are present in the seeds. Objective is to determine the content of bioactive compounds present in the black gram seeds and to study effect of sample pretreatment, fractionation on total alkaloid, total phenolic and total flavonoids content. Three types of extracts were prepared by using ethanol as a solvent by using Soxhlet extractor. First Ethanolic extract (EE), second e
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37

Nicola, Carla, Mirian Salvador, Adriana Escalona Gower, Sidnei Moura, and Sergio Echeverrigaray. "Chemical Constituents Antioxidant and Anticholinesterasic Activity ofTabernaemontana catharinensis." Scientific World Journal 2013 (2013): 1–10. http://dx.doi.org/10.1155/2013/519858.

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The present work aimed to analyze the alkaloid content of the ethanolic extract ofTabernaemontana catharinensis(Apocynaceae family) and its fractions as well as to evaluate their antioxidant and anticholinesterasic activities. The analyses of the ethanolic extract ofT. catharinensisby mass spectrometry allowed identifying the presence of the alkaloids 16-epi-affinine, coronaridine-hydroxyindolenine, voachalotine, voacristine-hydroxyindolenine, and 12-methoxy-n-methyl-voachalotine, as well as an alkaloid withm/z385.21 whose spectrum suggests a derivative of voacristine or voacangine. The extrac
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38

Yuliana, Rika, Anny Sartika Daulay, Ridwanto Ridwanto, and Fathur Rahman. "Determination Of Total Alkaloid Content Of Yellow Wood (Arcangelisia flava (L.) Merr) Extract Based On Differences In Ethanol Concentration Using Uv-Vis Spectrophotometry Method." Indonesian Journal of Science and Pharmacy 1, no. 2 (2023): 42–49. https://doi.org/10.63763/ijsp.v1i2.27.

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Yellow wood with its secondary metabolites is one of the plants that has the potential as a traditional medicinal plant to treat various diseases, such as cancer. The chemical compounds contained in yellow wood are alkaloids, saponins, tannins, flavonoids, steroids/triterpenoids. The objective of this research was to determine the chemical compounds contained in ethanol extract and determine the total alkaloid value of yellow wood extract. This research method is an experimental method which includes processing plant materials, making ethanol extract concentrations of 50, 70 and 96%. Character
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39

Sengnon, Narumon, Phanita Vonghirundecha, Wiraphon Chaichan, et al. "Seasonal and Geographic Variation in Alkaloid Content of Kratom (Mitragyna speciosa (Korth.) Havil.) from Thailand." Plants 12, no. 4 (2023): 949. http://dx.doi.org/10.3390/plants12040949.

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The objective of this study was to obtain data on the distribution of alkaloids in kratom plants grown in Thailand. Two collections were performed, covering the southern, central, and northern regions of Thailand and different seasons. The contents of alkaloids, including mitragynine (MG), paynantheine (PAY), and speciogynine (SG), were determined using the validated HPLC method. The 134 samples in the first collection were collected from Nam Phu subdistrict, Ban Na San, Surat Thani, Thailand, during June and October 2019 and January 2020. The maximum mitragynine content was 4.94% w/w in June
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40

Parra-Gallardo, Giovana, María del Carmen Salas-Sanjuán, Fernando del Moral, and Juan Luis Valenzuela. "Characterising the Nutritional and Alkaloid Profiles of Tarwi (Lupinus mutabilis Sweet) Pods and Seeds at Different Stages of Ripening." Agriculture 14, no. 10 (2024): 1812. http://dx.doi.org/10.3390/agriculture14101812.

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Tarwi (Lupinus mutabilis Sweet) is a key crop for Andean indigenous communities, offering proteins and fats. Both the pods and seeds of tarwi are consumed, either in their tender (immature) state or as dried, fully ripe seeds. Tarwi, like other Lupinus species, contains high alkaloid levels in its fruits and seeds that must be removed before consumption. This study evaluated the fat, protein, fibre, and alkaloid contents of four cultivars at five maturity stages ranging from 180 to 212 days after sowing. Samples of the pods and the seeds were analysed to determine their colour and protein, cru
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41

Metrouh-Amir, Hassiba, and Nadir Amir. "Impact of extraction method on alkaloid content and antioxidant activity of Matricaria pubescens." Croatian journal of food science and technology 15, no. 1 (2023): 83–95. http://dx.doi.org/10.17508/cjfst.2023.15.1.10.

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Matricaria pubescens is a medicinal plant much appreciated by the people of the Saharan regions and widely used to treat many diseases. The present study was devoted to evaluate the effect of extraction technique, pH and nature of solvent on alkaloid content and antioxidant activity of M. pubescens. Alkaloid extraction was carried out by applying two techniques, maceration extraction and Soxhlet extraction, using several solvents at different pH (alkaline chloroform, acid methanol, neutral methanol and neutral ethanol). In the present study, the antioxidant activity of the alkaloid extracts of
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42

Mishra, Malay Ranjan, Rajesh Kumar Srivastava, and Nasim Akhtar. "Enhanced Alkaloid Production from Cell Culture System of Catharanthus roseus in Combined Effect of Nutrient Salts, Sucrose and Plant Growth Regulators." Journal of Biotechnology and Biomedical Science 1, no. 4 (2018): 14–34. http://dx.doi.org/10.14302/issn.2576-6694.jbbs-18-2475.

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Callus and biomass culture of Catharanthus roseus L. were established to check for the presence of total alkaloid and its subsequent yield. Various treatments like strength of nutrient salts, sucrose concentrations and combinations of plant growth regulators (PGR’s) were applied to both MS and B5 in agar as well as suspension medium to test their effects on enhanced alkaloid content and its yield. There was no significant difference in any of the observable parameters of fresh wt, dry wt, alkaloid content, production, productivity and yield if the culture were treated similarly in both types o
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43

Martins, Alice, Michael Wink, Andreas Tei, and Amélia P. Rauter. "Quinolizidine Alkaloid Profiles of Two Taxa of Teline maderensis." Zeitschrift für Naturforschung C 58, no. 11-12 (2003): 776–78. http://dx.doi.org/10.1515/znc-2003-11-1203.

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Abstract The alkaloid composition of the aerial parts of two taxa of Teline maderensis was studied by capillary GLC and GLC-MS. N-Methylcytisine was the major alkaloid found in both plants. Contents of cytisine and lupanine were higher in T. maderensis var. paivae while anagyrine content was more pronounced in T. maderensis var. maderensis. The alkaloids dehydrocytisine, N-acetylcytisine and epibaptifoline appeared only in T. maderensis var. maderensis and N-formylcytisine was identified as a minor constituent in T. maderensis var. paivae, and detected only in trace amounts in the other variet
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44

Ahmad, Islamudin, Rissyelly Rissyelly, Agus Kurniawan, and Abdul Munim. "SCREENING OF EXTRACTION METHOD FOR ALKALOID ENRICHMENT OF PEPEROMIA PELLUCIDA (L.) KUNTH." Asian Journal of Pharmaceutical and Clinical Research 10, no. 7 (2017): 214. http://dx.doi.org/10.22159/ajpcr.2017.v10i7.18426.

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Objective: The aims of this study were to investigate the effect of extraction method modification of Peperomia pellucida (L.) Kunth herbs for alkaloid enrichment and thin layer chromatography (TLC) profile.Methods: The dried herbs were extracted with six different methods (including; method A, B, C, D, and E). The obtained extract solution was evaporated and was analyzed. Alkaloid content identified using Dragendorff spray reagent. The gravimetric method was used to determine of total alkaloid, and TLC profile was analyzed using a densitometer at a wavelength of 254 nm and 366 nm.Results: The
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45

Al-marzook, Farah A., and Rabab Omran. "CYTOTOXIC ACTIVITY OF ALKALOIDS EXTRACTED FROM THREE IRAQI PLANTS AGAINST BREAST CANCER CELL LINE." Asian Journal of Pharmaceutical and Clinical Research 10, no. 9 (2017): 78. http://dx.doi.org/10.22159/ajpcr.2017.v10i9.19134.

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Objectives: Screening for cytotoxic activity of total alkaloid extracts of Eucalyptus camaldulensis, Aloe vera, and Capparis spinosa against breast cancer cell line Michigan Cancer Foundation-7 (MCF-7) and nontumorigenic fetal hepatic cell line (WRL-68).Methods: The plant powders were extracted separately with 80% methanol and chloroform at pH 2 and 10. Total alkaloids were detected qualitatively by Mayer’s, Dragendorff’s, and Hager‘s reagents and estimated quantitatively by bromocresol green spectrophotometry depending on the atropine calibration curve. The cytotoxic activity was evaluated by
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Kizil, S., Ö. Tonҫer, and T. Sogut. "Effect of Different Nitrogen Doses on Some Agricultural Characteristics and Alkaloid Content of Hyoscyamus reticulatus L. and Hyoscyamus niger L." Cercetari Agronomice in Moldova 50, no. 2 (2017): 83–90. http://dx.doi.org/10.1515/cerce-2017-0017.

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AbstractAlkaloids, nitrogen containing basic substance, have a complex structure. They are one of the most important groups of secondary metabolites, which is synthesized in roots and transported to other organs. Since alkaloids are nitrogenous compounds, the availability of nitrogen is expected to play an important role in the biosynthesis and accumulation of alkaloids in plants. Nitrogen affects yield and quality of medicinal plants, therefore, growers usually apply large amount of nitrogen to obtain high yields. The objective of the present study was to determine yield, yield components and
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47

Tang, Meng, Chaohan Li, Cheng Zhang, et al. "SWATH-MS-Based Proteomics Reveals the Regulatory Metabolism of Amaryllidaceae Alkaloids in Three Lycoris Species." International Journal of Molecular Sciences 24, no. 5 (2023): 4495. http://dx.doi.org/10.3390/ijms24054495.

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Alkaloids are a class of nitrogen-containing alkaline organic compounds found in nature, with significant biological activity, and are also important active ingredients in Chinese herbal medicine. Amaryllidaceae plants are rich in alkaloids, among which galanthamine, lycorine, and lycoramine are representative. Since the difficulty and high cost of synthesizing alkaloids have been the major obstacles in industrial production, particularly the molecular mechanism underlying alkaloid biosynthesis is largely unknown. Here, we determined the alkaloid content in Lycoris longituba, Lycoris incarnata
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48

Nastišin, Ľuboš, Jozef Fejér, Richard Hercek, et al. "Effects of Plant Growth Regulators and Foliar Nutrients on the Alkaloid Content in Poppy Straw of Opium Poppy (Papaver somniferum L.)." International Journal of Plant Biology 16, no. 2 (2025): 66. https://doi.org/10.3390/ijpb16020066.

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Poppy (Papaver somniferum L.) is an important industrial plant worldwide. It is legally cultivated in seven countries around the world for the production of poppy straw or raw opium in India for alkaloid extraction. This work focused on testing different types of growth regulators and foliar nutrients to increase and stabilize basic alkaloids, mainly morphine in dry capsules. Field trials were carried out for three years (2021–2023). Selected preparations and their combinations were applied on the seeds before sowing and on the leaves during vegetation. The content of basic alkaloids in the dr
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49

Demeyer, K., and R. Dejaegere. "The Influence of the Ca2+/K+ Balance and Light Energy on Alkaloid Content and Parttioning in Datura stramonium." Australian Journal of Botany 45, no. 1 (1997): 81. http://dx.doi.org/10.1071/bt94049.

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The influence of a varying Ca2+/K+ balance on alkaloid production and accumulation in Datura stramonium var. tatula L. Torr. (thorn-apple) was examined. Plants were grown in culture on two different mineral media, that varied only in the proportions of the two cations. In addition, plants were cultured under two different light regimes to investigate a possible interaction between different ecophysiological parameters. When plants were grown under a higher light energy regime, more alkaloids accumulated in the plants during a certain stage of development, but also a higher proportion was found
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Kusnanto, Chelvin Ari, Andayana Puspitasari Gani, Subagus Wahyuono, and Nanang Fakhrudin. "Optimasi Penggunaan High Shear Mixer pada Pembuatan Fraksi Alkaloid dari Daun Awar-awar (Ficus septica) dengan Desain Faktorial." Jurnal Kefarmasian Indonesia 11, no. 2 (2021): 76–89. http://dx.doi.org/10.22435/jki.v11i2.4874.

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Awar-awar (Ficus septica) is an Indonesian anticancer plant that contains alkaloids as the active compound. The n-hexane insoluble fraction (FTLH) is alkaloid-containing fraction obtained from the ethanolic extract of F. septica leaves (EEDFS) by fractionation using n-hexane. High shear mixer (HSM) can be used to optimize the separation processes, including fractionation. The application of HSM in the fractionation of EEDFS is affected by fractionation duration, amount of solvent per gram extract, and strirring strength. Thus, these parameters must be optimized to obtain the optimum condition
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