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Artykuły w czasopismach na temat „Antimycobacterial Susceptibility Using BACTEC”

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Data publikacji: 3 czerwca 2025

Data aktualizacji: 31 lipca 2025

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1

Hannan, Abdul, Saira Munir, Muhammad Usman Arshad, and Nabila Bashir. "In Vitro Antimycobacterial Activity of Pakistani Beri Honey Using BACTEC MGIT 960." International Scholarly Research Notices 2014 (October 7, 2014): 1–4. http://dx.doi.org/10.1155/2014/490589.

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Background. Tuberculosis (TB) is a chronic bacterial disease. Mycobacterium tuberculosis, being the leading member of the MTB complex, is the main cause of tuberculosis worldwide. Tuberculosis is managed with combination of drugs: streptomycin, isoniazid, rifampicin, ethambutol, and pyrazinamide. Over the recent past years resistance against first line antituberculous drugs has emerged rapidly throughout the world resulting in MDR strains. The new threat in the management of MDR-TB is the development of resistance against second line drugs: aminoglycosides, polypeptides, fluoroquinolones, and

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Moore, Andrea V., Scott M. Kirk, Steven M. Callister, Gerald H. Mazurek, and Ronald F. Schell. "Safe Determination of Susceptibility of Mycobacterium tuberculosis to Antimycobacterial Agents by Flow Cytometry." Journal of Clinical Microbiology 37, no. 3 (1999): 479–83. http://dx.doi.org/10.1128/jcm.37.3.479-483.1999.

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We showed previously that susceptibility testing forMycobacterium tuberculosis labeled with fluorescein diacetate could be accomplished rapidly by using flow cytometry. However, safety was a major concern because mycobacteria were not killed prior to flow cytometric analysis. In this study, we developed a biologically safe flow cytometric susceptibility test that depends on detection and enumeration of actively growing M. tuberculosis organisms in drug-free and antimycobacterial agent-containing medium. The susceptibilities of 17 clinical isolates of M. tuberculosis to ethambutol, isoniazid, a

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3

Bergmann, John S., Geoffrey Fish, and Gail L. Woods. "Evaluation of the BBL MGIT (Mycobacterial Growth Indicator Tube) AST SIRE System for Antimycobacterial Susceptibility Testing of Mycobacterium tuberculosis to 4 Primary Antituberculous Drugs." Archives of Pathology & Laboratory Medicine 124, no. 1 (2000): 82–86. http://dx.doi.org/10.5858/2000-124-0082-eotbmm.

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Abstract Objective.—To evaluate the performance of the BBL MGIT (Mycobacterial Growth Indicator Tube) AST SIRE system for the antimycobacterial susceptibility testing of Mycobacterium tuberculosis to isoniazid (at a concentration equivalent to the lower concentration used for testing by the method of proportion), rifampin, ethambutol, and streptomycin. Design.—Thirty-one clinical isolates and 30 challenge strains provided by the Centers for Disease Control and Prevention (CDC) were tested by MGIT AST SIRE using 2 methods of inoculum preparation, and results were compared with those of the meth

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4

Zhurilo, A. A., A. I. Barbova, Yu A. Cherednik, et al. "COMPARISON OF GENEXPERT MTB/RIF AND GENOTYPE SYSTEMS WITH MTBDRPLUS STRIPS FOR DETECTION OF MUTATIONS THAT ARE ASSOCIATED WITH M. TUBERCULOSIS RESISTANCE TO RIFAMPICIN IN TUBERCULOSIS." Ukrainian Pulmonology Journal 30, no. 4 (2022): 34–41. http://dx.doi.org/10.31215/2306-4927-2022-30-4-34-41.

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COMPARISON OF GENEXPERT MTB/RIF AND GENOTYPE SYSTEMS WITH MTBDRPLUS STRIPS FOR DETECTION OF MUTATIONS, ASSOCIATED WITH M. TUBERCULOSIS RESISTANCE TO RIFAMPICIN IN TUBERCULOSIS A. A. Zhurilo, A. I. Barbova, Yu. A. Cherednik, P. S. Trofi mova, S. V. Mironchenko, O. V. Pavlova, A. V. Chernov, L. M. Sladkova Summary The aim was to analyze the level of compliance of two molecular genetic methods GeneXpert MTB/RIF and GenoTypeDRplus in determining the drug resistance of M. tuberculosis to rifampicin when detecting mutations in the RRDR region associated with drug resistance. Object and methods. We s

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Sakhelashvili, M. I., Z. I. Piskur, O. I. Sakhelashvili–Bil, et al. "Application of BI-V for contact children and adolescents from focies of multidrug-resistant tuberculous infection: evaluation of effectiveness." Tuberculosis, Lung Diseases, HIV Infection, no. 1 (March 15, 2023): 37–43. http://dx.doi.org/10.30978/tb-2023-1-37.

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Objective — to study the need of using the natural immunomodulator ВІ-V for contact children and adolescents from focies of multidrug-resistant tuberculous infection (MDR-TBI) for a non-specific immunoprevention of tuberculosis (TB). Materials and methods. A retrospective analysis of the medical records of 120 contacts (75 children and 45 adolescents) from focies of MDR-TBI and 102 adult with TB, who became the source of infection was carried out. Microbiological examination in adults included the detection of Mycobacterium tuberculosis (MBT) in sputum by smear microscopy, sowing of material o

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6

Sriram, D., K. Jyothi Mallika, and P. Yogeeswari. "Synthesis of Tetrahydro-2H-[1,3,5]thiadiazine-5-(4-pyridylcarboxamido)-2-thione with antitubercular activity." Scientia Pharmaceutica 72, no. 1 (2004): 35–41. http://dx.doi.org/10.3797/scipharm.aut-04-04.

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3-Substituted-5-(4-pyridylcarboxamide)tetrahydro-2H-[1,3,5]thiadizine-2-thione derivatives (1-9) were synthesized as derivatives of isoniazid (INH) to overcome the resistance developed with its therapeutic use. The structures were confirmed by their spectral and elemental analyses data. These derivatives revealed higher lipophilicity compared with INH. The antimycobacterial activity of the synthesized compounds and INH was evaluated in vitro against Mycobacterium tuberculosis H37Rv at 6.25 µg/ml in BACTEC 12B medium using the BACTEC 460 radiometric system. The derivatives exhibited antitubercu

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7

Collins, L. A., M. N. Torrero, and S. G. Franzblau. "Green Fluorescent Protein Reporter Microplate Assay for High-Throughput Screening of Compounds againstMycobacterium tuberculosis." Antimicrobial Agents and Chemotherapy 42, no. 2 (1998): 344–47. http://dx.doi.org/10.1128/aac.42.2.344.

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ABSTRACT An optimal assay for high-throughput screening for new antituberculosis agents would combine the microplate format and low cost of firefly luciferase reporter assays and redox dyes with the ease of kinetic monitoring inherent in the BACTEC system. The green fluorescent protein (GFP) of the jellyfish Aequorea victoria is a useful reporter molecule which requires neither substrates nor cofactors due to the intrinsically fluorescent nature of the protein. The gene encoding a red-shifted, higher-intensity GFP variant was introduced by electroporation into Mycobacterium tuberculosis H37Ra

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8

Arain, T. M., A. E. Resconi, M. J. Hickey, and C. K. Stover. "Bioluminescence screening in vitro (Bio-Siv) assays for high-volume antimycobacterial drug discovery." Antimicrobial Agents and Chemotherapy 40, no. 6 (1996): 1536–41. http://dx.doi.org/10.1128/aac.40.6.1536.

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Bioluminescence-based assays to indicate antimicrobial susceptibility have been developed and validated for recombinant strains of Mycobacterium tuberculosis, Mycobacterium bovis BCG, Mycobacterium avium, and Mycobacterium intracellulare expressing an integrated eukaryotic luciferase gene. MICs determined with these bioluminescence assays for several antimycobacterial agents, including isoniazid, ethambutol, rifampin, amikacin, streptomycin, ciprofloxacin, and clarithromycin, compared favorably with traditional BACTEC methods and visual estimations of the inhibitory end point. Assay methodolog

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9

Werngren, J., E. Sturegård, P. Juréen, K. Ängeby, S. Hoffner, and T. Schön. "Reevaluation of the Critical Concentration for Drug Susceptibility Testing of Mycobacterium tuberculosis against Pyrazinamide Using Wild-Type MIC Distributions andpncAGene Sequencing." Antimicrobial Agents and Chemotherapy 56, no. 3 (2011): 1253–57. http://dx.doi.org/10.1128/aac.05894-11.

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ABSTRACTPyrazinamide (PZA) is a potent first-line agent for the treatment of tuberculosis (TB) with activity also against a significant part of drug-resistantMycobacterium tuberculosisstrains. Since PZA is active only at acid pH, testing for susceptibility to PZA is difficult and insufficiently reproducible. The recommended critical concentration for PZA susceptibility (MIC, 100 mg/liter) used in the Bactec systems (460 and MGIT 960) has not been critically evaluated against wild-type MIC distributions in clinical isolates ofMycobacterium tuberculosis. Using the Bactec MGIT 960 system, we dete

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10

Piersimoni, Claudio, Domenico Nista, Stefano Bornigia, and Giuseppina De Sio. "Evaluation of a New Method for Rapid Drug Susceptibility Testing of Mycobacterium avium Complex Isolates by Using the Mycobacteria Growth Indicator Tube." Journal of Clinical Microbiology 36, no. 1 (1998): 64–67. http://dx.doi.org/10.1128/jcm.36.1.64-67.1998.

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The reliability of the Mycobacteria Growth Indicator Tube (MGIT [BBL]) for rapid drug susceptibility testing of Mycobacterium avium complex (MAC) isolates was evaluated. MICs of amikacin, clarithromycin, clofazimine, ethambutol, and rifabutin were determined by the MGIT system for 16 MAC strains. The results were compared with those obtained by the BACTEC broth macrodilution method. The turnaround times were 6 to 8 days (median, 7 days) for the MGIT and 5 to 7 days (median, 6 days) for the BACTEC system. Agreements with BACTEC system-determined MICs, within ±1 log2 dilution, were 100, 100, 88,

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11

Ruiz, P., F. J. Zerolo, and M. J. Casal. "Comparison of Susceptibility Testing of Mycobacterium tuberculosis Using the ESP Culture System II with That Using the BACTEC Method." Journal of Clinical Microbiology 38, no. 12 (2000): 4663–64. http://dx.doi.org/10.1128/jcm.38.12.4663-4664.2000.

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The ESP Culture System II was evaluated for its capacity to test the susceptibility of 389 cultures of Mycobacterium tuberculosis to streptomycin, rifampin, ethambutol, and isoniazid. Good agreement with results with the BACTEC TB 460 was found. ESP II is a reliable, rapid, and automated method for performing susceptibility testing.

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12

Tolchkov, V., Y. Hodzhev, B. Tsafarova, et al. "Drug susceptibility testing of Mycobacterium tuberculosis using next generation sequencing and Mykrobe software." Journal of microbiology, epidemiology and immunobiology 98, no. 6 (2022): 697–705. http://dx.doi.org/10.36233/0372-9311-191.

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Introduction. Mycobacterium tuberculosis is the causative agent of tuberculosis. Drug susceptibility testing is performed by phenotypic and molecular tests. Commonly used for phenotypic drug susceptibility testing is the automated BACTEC system in a liquid culture medium. Drug susceptibility by line probe molecular tests was introduced almost 15 years ago. Recently whole genome sequencing (WGS) analysis of M. tuberculosis strains demonstrated that genotyping of drug-resistance could be accurately performed. Several software tools were developed.Our study aimed to perform whole-genome sequencin

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13

Davies, A. P., O. J. Billington, T. D. McHugh, D. A. Mitchison, and S. H. Gillespie. "Comparison of Phenotypic and Genotypic Methods for Pyrazinamide Susceptibility Testing with Mycobacterium tuberculosis." Journal of Clinical Microbiology 38, no. 10 (2000): 3686–88. http://dx.doi.org/10.1128/jcm.38.10.3686-3688.2000.

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Mycobacterium tuberculosis converts pyrazinamide to its active form by using the enzyme pyrazinamidase. This enzyme is coded for on the pncA gene, and mutations in the pncAgene result in absence of active enzyme, conferring resistance to the drug pyrazinamide. We investigated 27 strains of Mycobacterium tuberculosis suspected of being multidrug resistant. Each isolate was tested for susceptibility to pyrazinamide by the BACTEC 460TB method, and 19 were pyrazinamide resistant. The presence of active pyrazinamidase enzyme was sought by using the Wayne assay, which was positive in all of the sens

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14

Keren, Metin. "Drug Resistance Tests for Mycobacterium Tuberculosis and Compatibility between Tests; From Past to Today." Medical Science and Discovery 11, no. 2 (2024): 44–47. http://dx.doi.org/10.36472/msd.v11i2.1127.

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Objective: The gold standard for pulmonary tuberculosis diagnosis is the demonstration of Mycobacterium tuberculosis bacilli. Drug susceptibility test results of the bacilli obtained are crucial in tuberculosis treatment management. The various tests used to determine drug susceptibility must yield the same result. In the present study, we aimed to evaluate the compatibility of drug susceptibility test results obtained by Löwenstein-Jensen (L-J) and BACTEC 460TB methods from the past to the present. Material and Methods: Sputum results from 79 patients suspected of multidrug-resistant pulmonar

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Domotenko, L. V., T. P. Morozova, I. G. Shemyakin, and A. P. Shepelin. "EXPERIENCE OF USING TB TEST KIT FOR THE RAPID DRUG SUSCEPTIBILITY TESTING OF M. TUBERCULOSIS." Russian Clinical Laboratory Diagnostics 65, no. 2 (2020): 122–30. http://dx.doi.org/10.18821/0869-2084-2020-65-2-122-130.

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The results of the comparative testing of the susceptibility of M. tuberculosis clinical strains to isoniazid, streptomycin, rifampicin and ethambutol using the TB test kit, developed in SCRAMB, (Obolensk) and the absolute concentrations method; the TB test kit and the BACTEC MGIT 960 automated system are presented in the study. A total of 629 and 220 strains, respectively, were tested. A high degree of agreement of the results was shown: 89.1-98.6% for isoniazid, 96.2-98.0% for rifampicin, 91.5-98.2% for streptomycin and 89.1-95.9% for ethambutol. The smallest number of discrepancies in the r

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16

Kadhim, Afraa Ali, Mohammed Faraj AL-Marjan, Kadhim Ali Kadhim, and Ahmed Asmar Manki. "Isolation of Mycobacterium tuberculosis and testing their susceptibility to antimicrobial agents by using Bactec 960." Al Mustansiriyah Journal of Pharmaceutical Sciences 13, no. 1 (2013): 175–81. http://dx.doi.org/10.32947/ajps.v13i1.213.

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This study was done from 3/7/2011 to 1/1/2012 , different clinical samples were collected from (1235) patients that coming to the Specialized center for chest and respiratory diseases / Baghdad to investigate Mycobacterium tuberculosis and study their resistant to first and second line antimicrobial agents by using Bactec 960.The results obtained from direct smear examination with Ziehl – Neelsen stain in (294) patients that indicated these patients are Infected with tuberculosis . These samples has been cultured on solid medium Lowenstein Jensen media (L.J.) and liquid medium ( middle brook 7

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17

Alcaide, Fernando, Laura Calatayud, Miguel Santín, and Rogelio Martín. "Comparative In Vitro Activities of Linezolid, Telithromycin, Clarithromycin, Levofloxacin, Moxifloxacin, and Four Conventional Antimycobacterial Drugs against Mycobacterium kansasii." Antimicrobial Agents and Chemotherapy 48, no. 12 (2004): 4562–65. http://dx.doi.org/10.1128/aac.48.12.4562-4565.2004.

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ABSTRACT Mycobacterium kansasii is one of the most pathogenic and frequent nontuberculous mycobacteria isolated from humans. Patients with adverse drug reactions, resistant isolates, or suboptimal response require alternative treatment regimens. One hundred forty-eight consecutive clinical isolates of M. kansasii were tested for antimicrobial susceptibilities by the BACTEC 460 system (NCCLS) with two different inoculation protocols, one conventional and one alternative. In the alternative protocol, the inoculum 12B vial was incubated until the growth index was between 250 and 500. Four convent

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Domotenko, L. V., T. P. Morozova, M. V. Khramov, and А. P. Shepelin. "Testing Susceptibility of M. tuberculosis to Second Line Anti-Tuberculosis Drugs Using the XDR Test in Clinical Trials and International Professional Testing Cycles." Tuberculosis and Lung Diseases 99, no. 8 (2021): 13–20. http://dx.doi.org/10.21292/2075-1230-2021-99-8-13-20.

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The objective of the study: to evaluate the commercial XDR test for susceptibility testing of M. tuberculosis to second line anti-tuberculosis drugs in clinical trials and as part of annual professional testing cycles coordinated by the World Health Organization (WHO).Subjects and Methods. Cultures of M. tuberculosis (n = 90) freshly isolated on egg media from clinical samples collected in tuberculosis patients were tested using the Bactec MGIT 960 system and the XDR test under identical conditions. Well-studied strains of M. tuberculosis (n = 216) obtained from the WHO supranational laborator

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Nosova, E. Yu, M. A. Sviridenko, A. A. Khakhalina, K. Yu Galkina, Yu D. Mikhailova, and S. G. Safonova. "Rapid diagnostics of tuberculosis using the Xpert MTB/Rif Ultra test." Tuberculosis and socially significant diseases 13, no. 1 (2025): 22–29. https://doi.org/10.54921/2413-0346-2025-13-1-22-29.

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The aim of the study was evaluation of the diagnostic efficiency of the Xpert MTB/RIF Ultra test for detection of M. tuberculosis DNA and determination of drug susceptibility to rifampicin in various diagnostic samples of patients examined for disease verification.Materials and methods. We analyzed 427 respiratory and extrapulmonary diagnostic samples obtained from 378 patients (including HIV-infected ones) requiring rapid TB diagnostics. The study was conducted from one portion of the sediment of the diagnostic sample using bacteriological methods (luminescent microscopy, cultivation on Middl

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Maningi, Nontuthuko E., Luke T. Daum, John D. Rodriguez, et al. "Improved Detection by Next-Generation Sequencing of Pyrazinamide Resistance in Mycobacterium tuberculosis Isolates." Journal of Clinical Microbiology 53, no. 12 (2015): 3779–83. http://dx.doi.org/10.1128/jcm.01179-15.

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The technical limitations of common tests used for detecting pyrazinamide (PZA) resistance inMycobacterium tuberculosisisolates pose challenges for comprehensive and accurate descriptions of drug resistance in patients with multidrug-resistant tuberculosis (MDR-TB). In this study, a 606-bp fragment (comprising thepncAcoding region plus the promoter) was sequenced using Ion Torrent next-generation sequencing (NGS) to detect associated PZA resistance mutations in 88 recultured MDR-TB isolates from an archived series collected in 2001. These 88 isolates were previously Sanger sequenced, with 55 (

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Thuansuwan, Waraporn, Charoen Chuchottaworn, Chie Nakajima, Yasuhiko Suzuki, and Nuntaree Chaichanawongsaroj. "Biphasic Medium Using Nicotinamide for Detection of Pyrazinamide Resistance in Mycobacterium tuberculosis." Antibiotics 13, no. 6 (2024): 563. http://dx.doi.org/10.3390/antibiotics13060563.

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Reliable drug susceptibility testing of pyrazinamide (PZA) is technically difficult, since PZA activity is pH sensitive. The aim of this study was to evaluate a biphasic medium assay (BMA) for the reliable detection of PZA resistance in Mycobacterium tuberculosis (MTB) using nicotinamide (NIC) as a surrogate for PZA and identifying the appropriate cut-off value for the assay. The PZA susceptibility of 122 multidrug-resistant tuberculosis (MDR-TB) isolates and 39 drug-susceptible tuberculosis (DS-TB) isolates was examined using the BMA with NIC at four different concentrations (250, 500, 1000,

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Mikhailova, Y. D., M. V. Makarova, L. Y. Krylova, et al. "Study of drug susceptibility of Mycobacterium tuberculosis to delamanid using BACTECTM MGITTM 960 automated system." Tuberculosis and socially significant diseases 12, no. 4 (2025): 25–35. https://doi.org/10.54921/2413-0346-2024-12-4-25-35.

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Aim. To study the susceptibility of clinical strains of M. tuberculosis complex (MTB) to delamanid using the automated Bactec MGIT system.Material and methods. We studied 79 M. tuberculosis isolates from 78 TB patients treated in 2017-2023. 39 MTB strains were susceptibile and 40 had different drug resistance profiles to anti-tubercular drugs. Minimum inhibitory concentrations (MIC) of delamanid in Middlebrook 7H9 liquid medium were determined using the Bactec MGIT system, the susceptibility results were assessed using the critical concentration of 0.06 μg/ml recommended by WHO. Whole genome s

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El-Sayed Zaki, Maysaa, and Tarek Goda. "Rapid phenotypic assay of antimycobacterial susceptibility pattern by direct mycobacteria growth indicator tube and phage amplified biological assay compared to BACTEC 460 TB." Tuberculosis 87, no. 2 (2007): 102–8. http://dx.doi.org/10.1016/j.tube.2006.05.002.

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Pitaloka, Dian Ayu Eka, and Elin Yulinah Sukandar. "IN VITRO STUDY OF URSOLIC ACID COMBINATION FIRST-LINE ANTITUBERCULOSIS DRUGS AGAINST DRUG-SENSITIVE AND DRUG-RESISTANT STRAINS OF Mycobacterium tuberculosis." Asian Journal of Pharmaceutical and Clinical Research 10, no. 4 (2017): 216. http://dx.doi.org/10.22159/ajpcr.2017.v10i4.16582.

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Objective: The resurgence of tuberculosis (TB) caused by Mycobacterium TB (MTB) is associated with the rapid spread of multidrug-resistant,therefore, the development of new antimycobacterial agents is necessary. The aim of this study was to evaluate the antimycobacterial activity ofursolic acid (UA) when it using alone and combination with TB drugs.Methods: MTB H37Rv strain, streptomycin-rifampicin resistant strain, and isoniazid-ethambutol resistant strain were evaluated by susceptibility testusing a serial number of UA (25-150 µg/mL). Minimum inhibitory concentration (MIC) was read as minimu

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Kim, Jae-Seok, Go-Eun Kang, Han-Sung Kim, Hyun Soo Kim, Wonkeun Song, and Kyu Man Lee. "Evaluation of Verigene Blood Culture Test Systems for Rapid Identification of Positive Blood Cultures." BioMed Research International 2016 (2016): 1–6. http://dx.doi.org/10.1155/2016/1081536.

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The performance of molecular tests using the Verigene Gram-Positive and Gram-Negative Blood Culture nucleic acid tests (BC-GP and BC-GN, resp.; Naosphere, Northbrook, IL, USA) was evaluated for the identification of microorganisms detected from blood cultures. Ninety-nine blood cultures containing Gram-positive bacteria and 150 containing Gram-negative bacteria were analyzed using the BC-GP and BC-GN assays, respectively. Blood cultures were performed using the Bactec blood culture system (BD Diagnostic Systems, Franklin Lakes, NJ, USA) and conventional identification and antibiotic-susceptibi

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Eliseev, P. I., E. I. Nikishova, A. Yu Krupskaya, V. I. Shtraukh, E. S. Khimova, and A. O. Maryandyshev. "Detection of <i>M. tuberculosis</i> Using Cartridge-Based Technology in Patients with Negative Sputum Microscopy Results in a Regional Laboratory." Tuberculosis and Lung Diseases 102, no. 2 (2024): 36–42. http://dx.doi.org/10.58838/2075-1230-2024-102-2-36-42.

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The objective: to evaluate results of M. tuberculosis detection and testing drug susceptibility to rifampicin using molecular genetic methods in tuberculosis patients with a negative sputum smear microscopy result.Subjects and Methods. Pulmonary tuberculosis patients registered in Arkhangelsk Region from 2016 to 2020 were enrolled in the study; those patients were notified as new cases and relapses with a negative sputum smear microscopy result before treatment and had results of molecular genetic testing (MGT) (Xpert MTB/RIF MTB/RIF) and BACTEC MGIT 960 available.Results. 479 people (40%) wer

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Andres, Sönke, Doris Hillemann, Sabine Rüsch-Gerdes, and Elvira Richter. "Occurrence ofrpoBMutations in Isoniazid-Resistant but Rifampin-Susceptible Mycobacterium tuberculosis Isolates from Germany." Antimicrobial Agents and Chemotherapy 58, no. 1 (2013): 590–92. http://dx.doi.org/10.1128/aac.01752-13.

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ABSTRACTFour out of 143 phenotypically isoniazid-resistant but rifampin-susceptibleMycobacterium tuberculosisstrains that were isolated from patients in Germany in 2011 had mutations in the rifampin resistance-determining region ofrpoB. After performing drug susceptibility testing (DST) with two methods, the proportion method on Löwenstein-Jensen medium and using the Bactec 960 Mycobacteria Growth Indicator Tube system, we conclude that the two methods are equally reliable for phenotypic DST and MIC determination.

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Singh, Ashok K., Shailendra K. Singh, Kavita Dhariyal, Ram Kumar, Amarendra Kumar, and Sudheer K. Singh. "Synthesis, Characterization and Antimycobacterial Activity of Phenanthrenequinone Thiosemicarbazones and their Ruthenium and Zinc Complexes." Asian Journal of Chemistry 33, no. 5 (2021): 983–88. http://dx.doi.org/10.14233/ajchem.2021.23127.

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The substituted phenanthrenequinone thiosemicarbazones (HL1, HL2 and HL3), and their metal complexes (M = Ru(II) or Zn(II); SM1, SM2, SM3 and SM4) were synthesized and characterized by FT-IR, 1H & 13C NMR and ESI-MS. The spectral data IR, 1H & 13C NMR and ESI-MS indicates two tridentate ligands coordinating in the form of an octahedral geometry. The gas phase geometry of all the zinc(II) and ruthenium(II) complexes was carried by using density functional theory (DFT). The antimycobacterial susceptibility testing at 100 μM of the complexes using resazurin microtiter plate assay resulted

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Ovsyankina, E. S., L. V. Panova, E. A. Krushinskaya, O. A. Piskunova, and F. G. Poluektova. "ON THE QUESTION OF REDUCED DURATION OF PULMONARY TB TREATMENT IN ADOLESCENTS WITH DRUG SUSCEPTIBILITY DETERMINATION DURING TREATMENT." Вестник ЦНИИТ 7, no. 2 (2023): 67–72. http://dx.doi.org/10.57014/2587-6678-2023-7-2-67-72.

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We carried out a cohort retrospective study to justify the possibilities to continue the main TB treatment course in adolescents with determined drug susceptibility during treatment (without return to treatment commencement) in order to reduce the total duration of treatment. Out of 123 patients who completed treatment in 2019–2022 we selected 31 patients with drug susceptibility test (DST) results obtained by only cultural studies using BACTEC MGIT 960 after 1.5-2 months of treatment. We established that DST results obtained using BACTEC MGIT 960 during treatment would determine further treat

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K, Kalaiselvi, Mangayarkarasi V, Gomathi Ns, Balaji S, Shivshankar R. Mane, and Raja Shunmugam. "LUCIFERASE REPORTER MYCOBACTERIOPHAGES FOR EVALUATING NORBORNENE-BASED ANTITUBERCULOSIS DRUG SUSCEPTIBILITY TESTING ON MYCOBACTERIUM TUBERCULOSIS." Asian Journal of Pharmaceutical and Clinical Research 10, no. 9 (2017): 406. http://dx.doi.org/10.22159/ajpcr.2017.v10i9.19660.

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Objective: In 2015, 9.6 million people around the world became sick with tuberculosis (TB) disease and 1.5 million TB-related deaths worldwide. Recent increasing incidence of multidrug-resistant (MDR; resistance to at least rifampicin (RIF) and isoniazid [INH]) and extensively drug-resistant (MDR resistance plus resistance to a fluoroquinolone and an aminoglycoside) makes TB a serious concern. Lot of research is needed to deal with this infectious disease for a better alternative in treatment or modification of these older TB drugs. The present study aimed at evaluating antimycobacterial activ

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A, Husain, Agarwal S, Jafar H, Awasthi S, and Prakash V. "Etiological agents causing paediatric sepsis and their antimicrobial susceptibility pattern at a tertiary care hospital." Journal of Medical and Scientific Research 13, no. 2 (2025): 131–35. https://doi.org/10.17727/jmsr.2024/13-23.

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Background: Paediatric septicemia causes significant global morbidity and mortality, particularly in resource-poor regions. The knowledge of microbiological profile of sepsis in children is utmost important to guide the treatment. This study aims to investigate the prevalent pathogens and their antimicrobial susceptibility pattern in cases of pediatric sepsis in a tertiary care hospital. Material and methods: In this cross-sectional study, a total of 321 Blood culture samples (BACTEC bottles) were collected from suspected sepsis patients aged 1 month to 18 years, admitted in the pediatric ward

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Howard, Wanita J., Barry J. Buschelman, Martha J. Bale, Michael A. Pfaller, Frank P. Koontz, and Ronald N. Jones. "Vitek GPS card susceptibility testing accuracy using direct inoculation from Bactec 9240 blood culture bottles." Diagnostic Microbiology and Infectious Disease 24, no. 2 (1996): 109–12. http://dx.doi.org/10.1016/0732-8893(95)00273-1.

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Chovatia, P. T., J. D. Akabari, P. K. Kachhadia, P. D. Zalavadia, and H. S. Joshi. "Synthesis and selective antitubercular and antimicrobial inhibitory activity of 1-acetyl-3,5-diphenyl-4,5-dihydro-(1h)-pyrazole derivatives." Journal of the Serbian Chemical Society 71, no. 7 (2006): 713–20. http://dx.doi.org/10.2298/jsc0607713c.

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The new compounds 1-aryl-3-{1-phenyl-3-[p (methylthio)phenyl]pyrazol-4-yl}-2-propen-1-ones 2a?l were prepared by the condensation of 1-phenyl-3-[p (methylthio)phenyl]-4-formylpyrazole 1 with different aryl ketones. Compounds 2a?l in reaction with hydrazine hydrate yielded 3-aryl-5-{1-phenyl-3-[p-(methylthio)phenyl]pyrazol-4 yl}-4,5-dihydro-(1H)-pyrazoles 3a?l and in the presence of hydrazine hydrate in glacial acetic acid gave 1-acetyl-3-aryl 5-{1-phenyl-3-[p-(methylthio)phenyl]pyrazol-4-yl}-4,5 dihydro-(1H)-pyrazoles 4a?l. These compounds were tested in vitro for their antitubercular and anti

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Klymnyuk, Sergiy I., Leonid A. Hryshchuk, Mukola O. Vynnychuk, et al. "DIAGNOSTIC CHEMORESISTANT MYCOBACTERIUM TUBERCULOSIS IN THE TERNOPIL REGION OF UKRAINE." Wiadomości Lekarskie 73, no. 5 (2020): 959–62. http://dx.doi.org/10.36740/wlek202005122.

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The aim: To analyze the changes in indicators of tuberculosis mycobacteria sensitivity to anti-mycobacterial drugs over the past nine years in Ternopol region (Ukraine) and to develop recommendations for the use of drug combinations in this region. Materials and methods: The medical examinations were carried out in the Clinical and Diagnostic Laboratory of Ternopil Regional Tuberculosis Hospital during 2007-2017. Sensitivity analysis was carried out on a solid Löwenstein-Jensen medium and in liquid Meedlebrook medium using automatic bacteriological BACTEC 1443 tests with addition to the media

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Halperin, Ana V., José Luis Cortés Cuevas, Juan Antonio Del Castillo Polo, et al. "11. Evaluation of the Bact/alert® VIRTUO™ in Terms of Time to Detection, Performance, Workflow Efficiency and Impact on Patient Management, Compared to the BACTEC™ FX Automated Blood Culture System." Open Forum Infectious Diseases 7, Supplement_1 (2020): S7. http://dx.doi.org/10.1093/ofid/ofaa417.010.

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Abstract Background Bloodstream infections are associated with high rates of morbidity and mortality, therefore prompt identification and antimicrobial susceptibility testing of the causative organism(s) are critical. We compared the microbiological/clinical performance of BacT/ALERT®-VIRTUO™-(BioMerieux) to that of the BACTEC™FX-(BD) instrument, with time-to-detection (TTD, from loading into system until positivity) as the primary outcome. Secondary microbiological outcomes were positivity and contamination rates, hands-on-time, turn-around-time (TAT) and time-to-identification. Methods We pe

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Nnamani, Kenechi O., Chioma P. Nnamani, Kenechukwu K. Iloh, et al. "Bacterial isolates, antibiogram and outcomes of blood culture proven sepsis in neonates at a tertiary institution in South East Nigeria: a cross-sectional study." Therapeutic Advances in Infectious Disease 9 (January 2022): 204993612211224. http://dx.doi.org/10.1177/20499361221122479.

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Background: Neonatal sepsis (NNS) in developing countries continues to be a diagnostic and management challenge due to the delays in identifying the aetiologic pathogens and antibiograms. Aim: To determine the predominant bacterial isolates, antibiotic susceptibility pattern and outcomes of blood culture proven sepsis in neonates. Methodology: A hospital-based cross-sectional study of 120 neonates admitted into the Special Care Baby Unit (SCBU) of Nnamdi Azikiwe University Teaching Hospital Nnewi with clinical features suggestive of sepsis. A semi-structured questionnaire and proforma were use

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FUURSTED, KURT. "Comparison of growth and susceptibility testing of pyrazinamide in different Bactec media using strains of theM. tuberculosiscomplex." APMIS 101, no. 1-6 (1993): 154–59. http://dx.doi.org/10.1111/j.1699-0463.1993.tb00095.x.

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Kurtoğlu, MG, H. Bozkurt, O. Tuncer, R. Kesli, and M. Berktas. "Distribution, Optimum Detection Time and Antimicrobial Susceptibility Rates of the Microorganisms Isolated from Blood Cultures over a 4-Year Time Period in a Turkish University Hospital and a Review of the International Literature." Journal of International Medical Research 36, no. 6 (2008): 1261–72. http://dx.doi.org/10.1177/147323000803600613.

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This study retrospectively examined 8986 blood cultures from patients over a 4-year time period in an eastern Turkish university hospital to determine the detection times and distribution of isolated microorganisms using the automated BACTEC™ 9050 and BACTEC™ 9120 systems. A total of 1914 (21.3%) blood cultures contained pathogenic microorganisms and 252 (2.8%) positive cultures were considered contaminated. Of all the cultures, 18 (0.2%) were false positives and 224 (2.5%) were false negatives. In cultures containing pathogenic microorganisms, Gram-positive and Gram-negative bacterial isolati

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Sakhelashvili, M. I., O. P. Kostyk, O. I. Sakhelashvili–Bil, Z. I. Piskur, and J. J. Didyk. "Peculiarities of the chemodrug resistance of M. tuberculosis to antituberculous medications among children and adolescents from multidrugresistant tuberculous focies." Tuberculosis, Lung Diseases, HIV Infection, no. 2 (June 17, 2022): 5–11. http://dx.doi.org/10.30978/tb-2022-2-5.

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Objective — to study the peculiarities of the resistance of M. tuberculosis (MTB) to antimycobacterial drugs (AMBD) among children and adolescents living in multidrug-resistant tuberculous focies. Materials and methods. 246 children were examined, 145 of them had drug-resistant tuberculosis (TB), 101 children — susceptible form of the specific process, and 102 adult patients who became the source of the disease. Microbiological study in children, adolescents and adults included: detection of MTB in sputum by smear microscopy, seeding on Levenstein—Jensen medium, typing of isolated MTB o

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Demers, Anne-Marie, Amour Venter, Sven O. Friedrich, et al. "Direct Susceptibility Testing of Mycobacterium tuberculosis for Pyrazinamide by Use of the Bactec MGIT 960 System." Journal of Clinical Microbiology 54, no. 5 (2016): 1276–81. http://dx.doi.org/10.1128/jcm.03162-15.

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Pyrazinamide (PZA) is a key antituberculosis drug, yet no rapid susceptibility test is commercially available. PZA drug susceptibility testing (DST) was performed directly on sputum samples from 327 patients and compared with the indirect method by using the Bactec MGIT 960 system in the context of patient screening for participation in a drug trial. Compared to standard indirect PZA DST, direct DST was successful in only 59% of cases, but results obtained were highly accurate and available faster. Agreement between the direct and indirect methods varied from 90 to 100% in each laboratory. The

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Suthum, Krairerk, Worada Samosornsuk, and Seksun Samosornsuk. "Characterization of katG, inhA, rpoB and pncA in Mycobacterium tuberculosis isolates from MDR-TB risk patients in Thailand." Journal of Infection in Developing Countries 14, no. 03 (2020): 268–76. http://dx.doi.org/10.3855/jidc.11974.

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Introduction: Multidrug-resistant tuberculosis (MDR-TB) is commonly found in Thailand especially in the public health region 5, the Western region of Thailand. This study’s aim was to characterize katG, inhA, rpoB and pncA genes in Mycobacterium tuberculosis. Methodology: One hundred strains of Mycobacterium tuberculosis (MTB) were isolated from sputum samples of MDR-TB risk patients in the laboratory of the Office of Disease Prevention and Control 5th Ratchaburi province, Thailand from January to December 2015. Drug susceptibility testing (DST) was performed using a BACTEC MGIT 960 syst

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Yakrus, Mitchell A., Jeffrey Driscoll, Allison McAlister, et al. "Molecular and Growth-Based Drug Susceptibility Testing ofMycobacterium tuberculosisComplex for Ethambutol Resistance in the United States." Tuberculosis Research and Treatment 2016 (2016): 1–5. http://dx.doi.org/10.1155/2016/3404860.

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Ethambutol (EMB) is used as a part of drug regimens for treatment of tuberculosis (TB). Susceptibility ofMycobacterium tuberculosiscomplex (MTBC) isolates to EMB can be discerned by DNA sequencing to detect mutations in theembBgene associated with resistance. US Public Health Laboratories (PHL) primarily use growth-based drug susceptibility test (DST) methods to determine EMB resistance. The Centers for Disease Control and Prevention (CDC) provides a service for molecular detection of drug resistance (MDDR) by DNA sequencing and concurrent growth-based DST using agar proportion. PHL and CDC te

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Pfyffer, Gaby E., Donald A. Bonato, Adeleh Ebrahimzadeh, et al. "Multicenter Laboratory Validation of Susceptibility Testing of Mycobacterium tuberculosis against Classical Second-Line and Newer Antimicrobial Drugs by Using the Radiometric BACTEC 460 Technique and the Proportion Method with Solid Media." Journal of Clinical Microbiology 37, no. 10 (1999): 3179–86. http://dx.doi.org/10.1128/jcm.37.10.3179-3186.1999.

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In a large multicenter study involving six major study sites in the United States, Canada, and Europe, the susceptibilities of 272Mycobacterium tuberculosis strains to classical second-line antituberculosis (anti-TB) drugs (capreomycin, cycloserine, ethionamide, and kanamycin) and newer compounds (amikacin, clofazimine, ofloxacin, and rifabutin) were determined by the radiometric BACTEC 460 procedure and the conventional proportion method on Middlebrook 7H10 agar. Previously established critical concentrations for classical second-line anti-TB drugs were compared with several concentrations in

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Tilahun, Melaku, Teklu Wegayehu, Biniam Wondale, et al. "Phenotypic and genotypic drug susceptibility patterns of Mycobacterium tuberculosis isolates from pulmonary tuberculosis patients in Central and Southern Ethiopia." PLOS ONE 18, no. 9 (2023): e0285063. http://dx.doi.org/10.1371/journal.pone.0285063.

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Introduction The persistence of tuberculosis (TB) infection in some patients after treatment has highlighted the importance of drug susceptibility testing (DST). This study aimed to determine the drug susceptibility patterns of Mycobacterium tuberculosis (M. tuberculosis) isolates from pulmonary TB (PTB) patients in Central and Southern Ethiopia. Methods A health institution-based cross-sectional study was conducted between July 2021 and April 2022. Sputum samples were collected from newly diagnosed smear microscopy and/or Xpert MTB/RIF-positive PTB patients. The samples were processed and cul

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Shifman, Ohad, Tamar Aminov, Moshe Aftalion, et al. "Evaluation of the European Committee on Antimicrobial Susceptibility Testing Guidelines for Rapid Antimicrobial Susceptibility Testing of Bacillus anthracis-, Yersinia pestis- and Francisella tularensis-Positive Blood Cultures." Microorganisms 9, no. 5 (2021): 1055. http://dx.doi.org/10.3390/microorganisms9051055.

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Rapid determination of bacterial antibiotic susceptibility is important for proper treatment of infections. The European Committee on Antimicrobial Susceptibility Testing (EUCAST) has recently published guidelines for rapid antimicrobial susceptibility testing (RAST) performed directly from positive blood culture vials. These guidelines, however, were only published for a limited number of common pathogenic bacteria. In this study, we evaluated the applicability of these guidelines to three Tier 1 bioterror agents (Bacillus anthracis, Yersinia pestis and Francisella tularensis) that require pr

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Chedore, Pamela, Lina Bertucci, Joyce Wolfe, Meenu Sharma, and Frances Jamieson. "Potential for Erroneous Results Indicating Resistance When Using the Bactec MGIT 960 System for Testing Susceptibility ofMycobacterium tuberculosisto Pyrazinamide." Journal of Clinical Microbiology 48, no. 1 (2009): 300–301. http://dx.doi.org/10.1128/jcm.01775-09.

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Borek, Anna, Anna Zabost, Agnieszka Głogowska, Dorota Filipczak, and Ewa Augustynowicz-Kopeć. "New RAPMYCOI SensititreTM Antimicrobial Susceptibility Test for Atypical Rapidly Growing Mycobacteria (RGM)." Diagnostics 12, no. 8 (2022): 1976. http://dx.doi.org/10.3390/diagnostics12081976.

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Rapidly growing mycobacteria (RGM) cause an increasing international concern, mainly due to their natural resistance to many antibiotics. The aim of this study was to conduct species identification and determine the antimicrobial susceptibility profiles of RGM isolated in Poland. Antimicrobial susceptibility was tested using broth microdilution and the RAPMYCOI panel. A total of 60 strains were analysed, including the following species: M. fortuitum complex (30), M. abscessus subsp. abscessus (16), M. abscessus subsp. massiliense (7), M. chelonae (5), and M. mucogenicum (2). For 12 M. abscessu

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Kania, Katarzyna, Katarzyna Wójcik, Kamil Drożdż, and Karolina Klesiewicz. "Utility of Rapid Molecular Assays for Detecting Multidrug-Resistant Mycobacterium tuberculosis in Extrapulmonary Samples." Diagnostics 15, no. 9 (2025): 1113. https://doi.org/10.3390/diagnostics15091113.

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Background: Extrapulmonary tuberculosis (TB) presents significant diagnostic challenges, particularly in the context of multidrug-resistant (MDR) strains. This study assessed the utility of the WHO-recommended rapid molecular assays, originally validated for pulmonary TB, in diagnosing extrapulmonary TB and detecting the MDR Mycobacterium tuberculosis complex (MTBC). Materials and Methods: A total of 6274 clinical samples, including 4891 pulmonary and 1383 extrapulmonary samples, were analyzed between 2019 and 2022 using the BD MAX™ MDR-TB assay (BD MAX), the Xpert® MTB/RIF assay (Xpert MTB/RI

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Kaya, Eyyüp, Hamide Kaya, and Candan Öztürk. "Antibiotic susceptibilities and microorganisms isolated from blood cultures of inpatients in a secondary state hospital in Türkiye." Cukurova Medical Journal 50, no. 1 (2025): 1–10. https://doi.org/10.17826/cumj.1548878.

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Purpose: The aim of this study was to evaluate the bacterial species distribution and antibiotic susceptibility in blood cultures obtained from patients in a secondary care center in Mersin, Türkiye. Materials and Methods: Blood culture specimens of inpatients sent to the microbiology laboratory between January 2022 and December 2023 were retrospectively analyzed to determine the etiologic agents of bloodstream infections and to evaluate their antimicrobial susceptibility. Samples were processed using the BD Bactec FX40 automated blood culture system (BD, USA), which allows continuous monitori

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Algazina, V. V., T. G. Smirnova, V. V. Romanov, and A. E. Ergeshov. "The incidence of M. tuberculosis resistance to linezolid and bedaquiline in patients for the period 2011-2022." Tuberculosis and socially significant diseases 12, no. 3 (2024): 20–25. https://doi.org/10.54921/2413-0346-2024-12-3-20-25.

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The objective. To analyze the incidence of linezolid- and bedaquiline-resistant strains of M. tuberculosis from smear-positive TB patients in 2011–2022.Subjects and Methods. A total of 3637 clinical strains were obtained from patients undergoing treatment in the CTRI in 2011-2022. Drug susceptibility for linezolid and bedaquiline were determined using Bactec MGIT 960 (Becton Dickinson, USA) with a critical concentration of 1 mg/L.Results. 101 patients with linezolid- and bedaquiline-resistant strains were detected. The incidence of linezolid-resistance increases from 0,2% in 2011 to 5,5% in 20

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