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Artykuły w czasopismach na temat „Bactec 460 tb system”

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Data publikacji: 3 czerwca 2025
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1

Hanna, Bruce A., Adeleh Ebrahimzadeh, L. Bruce Elliott, et al. "Multicenter Evaluation of the BACTEC MGIT 960 System for Recovery of Mycobacteria." Journal of Clinical Microbiology 37, no. 3 (1999): 748–52. http://dx.doi.org/10.1128/jcm.37.3.748-752.1999.

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We evaluated the BACTEC MGIT 960 system, which is a fully automated, noninvasive system for the growth and detection of mycobacteria with a capacity to incubate and continuously monitor 960 7-ml culture tubes. We studied 3,330 specimens, 2,210 respiratory and 1,120 nonrespiratory specimens, collected from 2,346 patients treated at six sites. Processed specimens were inoculated into the BACTEC MGIT 960 and BACTEC 460 TB systems, as well as onto Lowenstein-Jensen slants and Middlebrook 7H11/7H11 selective plates. From all culture systems, a total of 362 isolates of mycobacteria were recovered; t
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2

Brunello, Francesca, Flavio Favari, and Roberta Fontana. "Comparison of the MB/BacT and BACTEC 460 TB Systems for Recovery of Mycobacteria from Various Clinical Specimens." Journal of Clinical Microbiology 37, no. 4 (1999): 1206–9. http://dx.doi.org/10.1128/jcm.37.4.1206-1209.1999.

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A total of 1,830 specimens (75.7% respiratory and 24.3% nonrespiratory) were cultured in parallel with the MB/BacT and BACTEC 460 TB systems and on Lowenstein-Jensen (LJ) medium. Mycobacteria were identified from 173 (6.5%) specimens. The most common species recovered were Mycobacterium tuberculosis complex (65.9%),Mycobacterium avium complex (22.5%), andMycobacterium chelonae (9.2%). The recovery rates by individual systems were 96.5, 99.4, and 95.9% for MB/BacT, BACTEC 460 TB, and LJ medium, respectively, for all mycobacteria; the recovery rates were 99.1, 100, and 98.2%, respectively, for M
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3

Murray, Patrick R. "Mycobacterial cross-contamination with the modified BACTEC 460 TB system." Diagnostic Microbiology and Infectious Disease 14, no. 1 (1991): 33–34. http://dx.doi.org/10.1016/0732-8893(91)90085-t.

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4

Roggenkamp, Andreas, Mathias W. Hornef, Adelheid Masch, Bettina Aigner, Ingo B. Autenrieth, and Jürgen Heesemann. "Comparison of MB/BacT and BACTEC 460 TB Systems for Recovery of Mycobacteria in a Routine Diagnostic Laboratory." Journal of Clinical Microbiology 37, no. 11 (1999): 3711–12. http://dx.doi.org/10.1128/jcm.37.11.3711-3712.1999.

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MB/BacT, BACTEC 460 TB, and Löwenstein-Jensen (LJ) medium were evaluated in parallel for recovery of mycobacteria from 3,700 continuous clinical specimens in a routine laboratory. Mycobacteria were identified from 123 (3.3%) specimens. The recovery rates for all mycobacteria by the different systems were 91.0, 73.0, and 53.6% for BACTEC 460 TB, MB/BacT, and LJ medium, respectively. The recovery rates for Mycobacterium tuberculosis complex were 97.1, 80.2, and 67.6%, respectively. The lack of sensitivity of the MB/BacT system was more pronounced with smear-negative specimens and resulted in a
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5

Lakshmi, V., MA Patil, K. Subhadha, and V. Himabindu. "Isolation of mycobacteria by Bactec 460 TB system from clinical specimens." Indian Journal of Medical Microbiology 24, no. 2 (2006): 124. http://dx.doi.org/10.4103/0255-0857.25197.

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6

Rodrigues, CS, SV Shenai, DVG Almeida, et al. "Use of bactec 460 TB system in the diagnosis of tuberculosis." Indian Journal of Medical Microbiology 25, no. 1 (2007): 32. http://dx.doi.org/10.4103/0255-0857.31059.

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7

Rodrigues, CS, SV Shenai, DVG Almeida, et al. "USE OF BACTEC 460 TB SYSTEM IN THE DIAGNOSIS OF TUBERCULOSIS." Indian Journal of Medical Microbiology 25, no. 1 (2007): 32–36. http://dx.doi.org/10.1016/s0255-0857(21)02231-3.

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8

Bignardi, G. "False-positive Mycobacterium avium-intracellulare cultures with the Bactec 460 TB system." Journal of Hospital Infection 26, no. 3 (1994): 203–10. http://dx.doi.org/10.1016/0195-6701(94)90043-4.

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9

Nogales, Carmen, Samuel Bernal, and Mónica Chávez. "Comparison of the MB/BacT and BACTEC 460 TB Systems." Journal of Clinical Microbiology 37, no. 10 (1999): 3432–33. http://dx.doi.org/10.1128/jcm.37.10.3432-3433.1999.

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10

Ruiz, P., F. J. Zerolo, and M. J. Casal. "Comparison of Susceptibility Testing of Mycobacterium tuberculosis Using the ESP Culture System II with That Using the BACTEC Method." Journal of Clinical Microbiology 38, no. 12 (2000): 4663–64. http://dx.doi.org/10.1128/jcm.38.12.4663-4664.2000.

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The ESP Culture System II was evaluated for its capacity to test the susceptibility of 389 cultures of Mycobacterium tuberculosis to streptomycin, rifampin, ethambutol, and isoniazid. Good agreement with results with the BACTEC TB 460 was found. ESP II is a reliable, rapid, and automated method for performing susceptibility testing.
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11

Maningi, Nontuthuko E., Luke T. Daum, John D. Rodriguez, et al. "Improved Detection by Next-Generation Sequencing of Pyrazinamide Resistance in Mycobacterium tuberculosis Isolates." Journal of Clinical Microbiology 53, no. 12 (2015): 3779–83. http://dx.doi.org/10.1128/jcm.01179-15.

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The technical limitations of common tests used for detecting pyrazinamide (PZA) resistance inMycobacterium tuberculosisisolates pose challenges for comprehensive and accurate descriptions of drug resistance in patients with multidrug-resistant tuberculosis (MDR-TB). In this study, a 606-bp fragment (comprising thepncAcoding region plus the promoter) was sequenced using Ion Torrent next-generation sequencing (NGS) to detect associated PZA resistance mutations in 88 recultured MDR-TB isolates from an archived series collected in 2001. These 88 isolates were previously Sanger sequenced, with 55 (
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12

Werngren, J., E. Sturegård, P. Juréen, K. Ängeby, S. Hoffner, and T. Schön. "Reevaluation of the Critical Concentration for Drug Susceptibility Testing of Mycobacterium tuberculosis against Pyrazinamide Using Wild-Type MIC Distributions andpncAGene Sequencing." Antimicrobial Agents and Chemotherapy 56, no. 3 (2011): 1253–57. http://dx.doi.org/10.1128/aac.05894-11.

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ABSTRACTPyrazinamide (PZA) is a potent first-line agent for the treatment of tuberculosis (TB) with activity also against a significant part of drug-resistantMycobacterium tuberculosisstrains. Since PZA is active only at acid pH, testing for susceptibility to PZA is difficult and insufficiently reproducible. The recommended critical concentration for PZA susceptibility (MIC, 100 mg/liter) used in the Bactec systems (460 and MGIT 960) has not been critically evaluated against wild-type MIC distributions in clinical isolates ofMycobacterium tuberculosis. Using the Bactec MGIT 960 system, we dete
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13

Somoskövi, Ákos, Csaba Ködmön, Ákos Lantos, et al. "Comparison of Recoveries of Mycobacterium tuberculosis Using the Automated BACTEC MGIT 960 System, the BACTEC 460 TB System, and Löwenstein-Jensen Medium." Journal of Clinical Microbiology 38, no. 6 (2000): 2395–97. http://dx.doi.org/10.1128/jcm.38.6.2395-2397.2000.

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14

Somoskövi, Ákos, Csaba Ködmön, Ákos Lantos, et al. "Comparison of Recoveries of Mycobacterium tuberculosis Using the Automated BACTEC MGIT 960 System, the BACTEC 460 TB System, and Löwenstein-Jensen Medium." Journal of Clinical Microbiology 38, no. 6 (2000): 2395–97. http://dx.doi.org/10.1128/.38.6.2395-2397.2000.

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15

Baveja, C. P., R. Saksena, S. Kumar, H. S. Hira, and A. Khanna. "Detection of multidrug resistant tuberculosis using Malachite Green Microtube assay and BACTEC 460 TB system." International Journal of Infectious Diseases 16 (June 2012): e277. http://dx.doi.org/10.1016/j.ijid.2012.05.938.

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16

Sanguinetti, M., B. Posteraro, F. Ardito, et al. "Routine Use of PCR–Reverse Cross-Blot Hybridization Assay for Rapid Identification ofMycobacterium Species Growing in Liquid Media." Journal of Clinical Microbiology 36, no. 6 (1998): 1530–33. http://dx.doi.org/10.1128/jcm.36.6.1530-1533.1998.

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A PCR–reverse cross-blot hybridization assay procedure that is able to rapidly identify 13 species of clinically relevant mycobacteria was evaluated for routine use in the identification of acid-fast isolates growing in BACTEC 460 TB (12B and 13A) and BACTEC 9000 MB (Myco/F) liquid media. Eight of the probes used were already described by Kox et al. (L. F. F. Kox et al., J. Clin. Microbiol. 33:3225–3233, 1995). In addition, we used six other probes specific for M. chelonae, M. malmoense orM. szulgai, M. genavense,M. gordonae, M. terrae, andM. marinum/M. ulcerans that we designed ourselves. Thi
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17

TOMIOKA, Haruaki, Hajime SAITO, and Katsumasa SATO. "Evaluation of BACTEC 460 TB System for Measurement of In Vitro Anti-Mycobacterium leprae Activity of Various Antimicrobials." Japanese journal of leprosy 61, no. 3 (1992): 157–64. http://dx.doi.org/10.5025/hansen1977.61.157.

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18

Keren, Metin. "Drug Resistance Tests for Mycobacterium Tuberculosis and Compatibility between Tests; From Past to Today." Medical Science and Discovery 11, no. 2 (2024): 44–47. http://dx.doi.org/10.36472/msd.v11i2.1127.

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Objective: The gold standard for pulmonary tuberculosis diagnosis is the demonstration of Mycobacterium tuberculosis bacilli. Drug susceptibility test results of the bacilli obtained are crucial in tuberculosis treatment management. The various tests used to determine drug susceptibility must yield the same result. In the present study, we aimed to evaluate the compatibility of drug susceptibility test results obtained by Löwenstein-Jensen (L-J) and BACTEC 460TB methods from the past to the present. Material and Methods: Sputum results from 79 patients suspected of multidrug-resistant pulmonar
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19

Ashish, Kumar Jaiswal, and Jain Vidushi. "Cutaneous Tuberculosis – Where Children Are Placed." International Journal of Pharmaceutical and Clinical Research 15, no. 5 (2023): 651–65. https://doi.org/10.5281/zenodo.12574424.

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Cutaneous tuberculosis continues to be a significant medical problem even with the advent of highly effective antituberculous drugs. It constitutes about 1.5% of all extrapulmonary tuberculosis. The prevalence in children varies from 18 to 54% in India. There is no gender predilection and the infection occurs with increased frequency in 10-14 year age group. Intrafamilial source of TB has been observed very frequently. A concomitant TB lymphadenitis is most common while involvement of other Systemic organs like lung, bone and abdomen has also been served. Protective efficacy of BCG is debatabl
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20

Kharatmal, Shivsharan, Sarbjit Singh Jhamb, and Prati Pal Singh. "Evaluation of BACTEC 460 TB system for rapid in vitro screening of drugs against latent state Mycobacterium tuberculosis H37Rv under hypoxia conditions." Journal of Microbiological Methods 78, no. 2 (2009): 161–64. http://dx.doi.org/10.1016/j.mimet.2009.05.008.

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21

BILGIN, SALIH, MEFTUN UNSAL, HASAN HAMZA CEBI, and ALPER AKGUNES. "Resistance for Anti-Tuberculosis Drugs in Central Black Sea Region of Turke." Polish Journal of Microbiology 59, no. 2 (2010): 125–28. http://dx.doi.org/10.33073/pjm-2010-019.

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One of the primary aims in tuberculosis (TB) management is to detect new cases as early as possible, and instigate the most appropriate therapy, for which it is important to know the characteristics of TB drug resistance in society. The aim of our study was to determine the resistance status of tuberculosis in the Samsun region of Turkey. To achieve that, the medical records of 1,029 pulmonary tuberculosis patients admitted to Samsun Chest Diseases and Chest Surgery Hospital between 2004 and 2006 were analyzed for drug resistance characteristics. In order to define the problem, isolates were t
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22

Wilson, Michael L., Barbara L. Stone, Mary V. Hildred, and Randall R. Reves. "Prolonged incubation of blood and bone marrow cultures in 12B bottles processed on the BACTEC 460 TB system does not increase microbial recovery." Diagnostic Microbiology and Infectious Disease 25, no. 3 (1996): 113–15. http://dx.doi.org/10.1016/s0732-8893(96)00122-8.

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Bergmann, John S., William E. Keating, and Gail L. Woods. "Clinical Evaluation of the BDProbeTec ET System for Rapid Detection of Mycobacterium tuberculosis." Journal of Clinical Microbiology 38, no. 2 (2000): 863–65. http://dx.doi.org/10.1128/jcm.38.2.863-865.2000.

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The performance of the BDProbeTec ET system (BD Biosciences, Sparks, Md.) for direct detection of Mycobacterium tuberculosis complex (MTBC) in respiratory specimens was evaluated by comparing results to those of conventional mycobacterial culture performed with the BACTEC 460 TB system and Middlebrook 7H11 biplates. Patients known to have been on antituberculous therapy were excluded from the analysis. Of 600 evaluable specimens (4 specimens were excluded from the analysis due to failure of the internal amplification control [IAC]) from 332 patients, 57 grew mycobacteria; 16 were MTBC (from 12
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24

Aggarwal, Puneet, Archana Singal, Sambit Nath Bhattacharya, and Kiran Mishra. "Comparison of the radiometric BACTEC 460 TB culture system and Löwenstein–Jensen medium for the isolation of mycobacteria in cutaneous tuberculosis and their drug susceptibility pattern." International Journal of Dermatology 47, no. 7 (2008): 681–87. http://dx.doi.org/10.1111/j.1365-4632.2008.03675.x.

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Arbab Nasr, Mohamed H., Abdullahi Nor Hasan, Tarig M. S Alnour, and Eltayib H. Ahmed. "Evaluation of Newly Modified Potassium Nitrate Containing Media in Detection and Antibiogram of Mycobacterium tuberculosis." SVOA Microbiology 5, no. 4 (2024): 122–30. http://dx.doi.org/10.58624/svoamb.2024.05.049.

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Background: Mycobacterium is a genus of Actinobacteria, given its own family, the Mycobacteriaceae. The genus includes pathogens known to cause serious diseases in mammals, including tuberculosis (Mycobacterium tuberculosis) and the classic Hansen's strain of leprosy (Mycobacterium leprae) [1]. Tuberculosis is the most common life-threatening opportunistic infection (OI) and AIDS defining illness in developing countries [2]. The routine identification methods of tuberculosis includes, Tuberculin skin test, Chest X-ray, Growth and physical characteristics of the bacteria, automated based system
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Badawneh, Muwaffag, and Jalal Aljamal. "SYNTHESIS AND ANTITUBERCULAR ACTIVITY OF PIPERIDINE AND MORPHOLINE 1, 8 NAPHTHYRIDINE ANALOGUES." International Journal of Pharmacy and Pharmaceutical Sciences 8, no. 12 (2016): 252. http://dx.doi.org/10.22159/ijpps.2016v8i12.13503.

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<p><strong>Objective: </strong>The search for new, potentially useful antimycobacterial agents. In continuation with our previous screening for the discovery of novel drugs for tuberculosis, a new series of 1,8-naphthyridines derivatives were synthesized and evaluated <em>in vitro </em>for antimycobacterial activity against <em>Mycobacterium tuberculosis </em>H37Rv.</p><p><strong>Methods: </strong>Several 4-morpholinomethyl-1.8-naphthyridine derivatives have been synthesized in excellent yields. The synthesized compounds were ch
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27

Laverdiere, Michel, Louise Poirier, Karl Weiss, Claire Béliveau, Lucie Bédard, and Diane Desnoyers. "Comparative evaluation of the MB/BacT and BACTEC 460 TB systems for the detection of mycobacteria from clinical specimens: clinical relevance of higher recovery rates from broth-based detection systems☆." Diagnostic Microbiology and Infectious Disease 36, no. 1 (2000): 1–5. http://dx.doi.org/10.1016/s0732-8893(99)00103-0.

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Gegin, Savaş, and Esra Arslan Aksu. "Demographic and microbiological characteristics and mortality status of patients diagnosed with tuberculosis and treated between 2018 and 2023." Pamukkale Medical Journal 18, no. 3 (2025): 638–46. https://doi.org/10.31362/patd.1581370.

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Purpose: We aimed to determine the demographic data, radiological findings, comorbid conditions, antituberculosis drug resistance rates, and tuberculosis-related mortality of patients with pulmonary and pleural tuberculosis where mycobacterium tuberculosis growth was detected in culture. Materials and methods: Data from 439 patients diagnosed with pulmonary and pleural tuberculosis in the chest diseases hospital clinic between January 2018 and December 2023 were retrospectively evaluated. Patients whose sputum, bronchial lavage and pleural fluid samples were positive for Mycobacterium tubercul
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29

Bergmann, John S., and Gail L. Woods. "Evaluation of the ESP Culture System II for Testing Susceptibilities of Mycobacterium tuberculosis Isolates to Four Primary Antituberculous Drugs." Journal of Clinical Microbiology 36, no. 10 (1998): 2940–43. http://dx.doi.org/10.1128/jcm.36.10.2940-2943.1998.

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The reliability of the ESP Culture System II (herein referred to as ESP II) for testing susceptibilities of Mycobacterium tuberculosis isolates to isoniazid, rifampin, ethambutol, and streptomycin was evaluated by comparing results to those of the method of proportion (MOP), which was considered the reference method, for 20 clinical isolates and 30 challenge strains provided by the Centers for Disease Control and Prevention (CDC). Clinical isolates also were tested with the BACTEC TB 460 system; these results agreed with those obtained by the MOP for all isolates and all drugs, except the high
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Mokaddas, Eiman, Suhail Ahmad, Hanaa S. Eldeen, and Noura Al-Mutairi. "Discordance between Xpert MTB/RIF Assay and Bactec MGIT 960 Culture System for Detection of Rifampin-Resistant Mycobacterium tuberculosis Isolates in a Country with a Low Tuberculosis (TB) Incidence." Journal of Clinical Microbiology 53, no. 4 (2015): 1351–54. http://dx.doi.org/10.1128/jcm.03412-14.

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Among 452 samples that were positive by the Xpert MTB/RIF (Xpert) assay and MGIT 960 system (MGIT), 440 and 10Mycobacterium tuberculosissamples were detected as rifampin susceptible and rifampin resistant, respectively. Two isolates that were rifampin susceptible by the MGIT system were rifampin resistant by the Xpert assay.rpoBsequencing identified a silent (CTG521TTG) mutation in one isolate and a missense (GAC516TAC) mutation in another. The detection of rifampin resistance is imperfect with both the Xpert assay and MGIT system. Any discordant rifampin resistance results should be confirmed
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31

Benjamin, W. H., K. B. Waites, A. Beverly, et al. "Comparison of the MB/BacT System with a Revised Antibiotic Supplement Kit to the BACTEC 460 System for Detection of Mycobacteria in Clinical Specimens." Journal of Clinical Microbiology 36, no. 11 (1998): 3234–38. http://dx.doi.org/10.1128/jcm.36.11.3234-3238.1998.

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The MB/BacT system (MB/BacT) with a revised antibiotic supplement kit was compared with the BACTEC 460 system (BACTEC 460) in a test of 488 specimens submitted for mycobacterial culture from 302 patients. Twenty-four Mycobacterium tuberculosis isolates were detected by the BACTEC 460 versus 23 isolates by the MB/BacT. Mean time until detection of M. tuberculosis isolates identified by both systems was 11.9 days for the BACTEC 460 versus 13.7 days for the MB/BacT (P = 0.046). M. aviumcomplex was detected in 12 specimens by the MB/BacT versus 10 specimens by the BACTEC 460. Only 8 of 14 (57%) M.
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Zaki, Maysaa El-Sayed, and Samir Abou-El Hassan. "Clinical Evaluation of Gen-Probe's Amplified Mycobacterium Tuberculosis Direct Test for Rapid Diagnosis of Mycobacterium tuberculosis in Egyptian Children at Risk for Infection." Archives of Pathology & Laboratory Medicine 132, no. 2 (2008): 244–47. http://dx.doi.org/10.5858/2008-132-244-ceogam.

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Abstract Context.—Diagnostic detection of tuberculosis (TB) has improved considerably. Available, standardized, nucleic acid–based amplification techniques have been shown to yield reliable results within 4 to 7 hours of sample processing. Objective.—To study the diagnostic performance of Gen-Probe's technique for direct detection of Mycobacterium tuberculosis in comparison with BACTEC 460 TB culture for both positive and negative Ziehl-Neelsen smears in Egyptian children at risk for TB infection. Design.—We prospectively evaluated 50 children from families with a positive history of TB. All p
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Tortoli, Enrico, Paola Cichero, Claudio Piersimoni, M. Tullia Simonetti, Giampietro Gesu, and Domenico Nista. "Use of BACTEC MGIT 960 for Recovery of Mycobacteria from Clinical Specimens: Multicenter Study." Journal of Clinical Microbiology 37, no. 11 (1999): 3578–82. http://dx.doi.org/10.1128/jcm.37.11.3578-3582.1999.

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The BACTEC MGIT 960 instrument is a fully automated system that exploits the fluorescence of an oxygen sensor to detect growth of mycobacteria in culture. Its performance was compared to those of the radiometric BACTEC 460 instrument and egg-based Lowenstein-Jensen medium. An identical volume of sample was inoculated in different media, and incubation was carried out for 6 weeks with the automatic systems and for 8 weeks on solid media. A total of 2,567 specimens obtained from 1,631 patients were cultured in parallel. Mycobacteria belonging to nine different taxa were isolated by at least one
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Bergmann, John S., Geoffrey Fish, and Gail L. Woods. "Evaluation of the BBL MGIT (Mycobacterial Growth Indicator Tube) AST SIRE System for Antimycobacterial Susceptibility Testing of Mycobacterium tuberculosis to 4 Primary Antituberculous Drugs." Archives of Pathology & Laboratory Medicine 124, no. 1 (2000): 82–86. http://dx.doi.org/10.5858/2000-124-0082-eotbmm.

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Abstract Objective.—To evaluate the performance of the BBL MGIT (Mycobacterial Growth Indicator Tube) AST SIRE system for the antimycobacterial susceptibility testing of Mycobacterium tuberculosis to isoniazid (at a concentration equivalent to the lower concentration used for testing by the method of proportion), rifampin, ethambutol, and streptomycin. Design.—Thirty-one clinical isolates and 30 challenge strains provided by the Centers for Disease Control and Prevention (CDC) were tested by MGIT AST SIRE using 2 methods of inoculum preparation, and results were compared with those of the meth
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35

Heifets, L., T. Linder, T. Sanchez, D. Spencer, and J. Brennan. "Two Liquid Medium Systems, Mycobacteria Growth Indicator Tube and MB Redox Tube, for Mycobacterium tuberculosis Isolation from Sputum Specimens." Journal of Clinical Microbiology 38, no. 3 (2000): 1227–30. http://dx.doi.org/10.1128/jcm.38.3.1227-1230.2000.

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Two manual liquid medium systems, the Mycobacteria Growth Indicator Tube (MGIT) and MB Redox tube systems, were evaluated in comparison to the radiometric BACTEC-460 semiautomated system for recovery ofMycobacterium tuberculosis from sputum specimens. The highest level of recovery, from a total of 77 culture-positive specimens, occurred with the BACTEC-460 system (92.2%), followed by the MB Redox tube (80.5%) and the MGIT (63.6%) systems. The shortest time to detection was observed also among the cultures in BACTEC-460: a mean of 12 days to a growth index (GI) of 10 and 15 days to a GI of 500.
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36

Spark, Ronald P., and Mary L. Fried. "Negative BACTEC 460-TB® Cultures: How Long to Incubate?" American Journal of Clinical Pathology 90, no. 2 (1988): 213–15. http://dx.doi.org/10.1093/ajcp/90.2.213.

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Pfyffer, Gaby E., Donald A. Bonato, Adeleh Ebrahimzadeh, et al. "Multicenter Laboratory Validation of Susceptibility Testing of Mycobacterium tuberculosis against Classical Second-Line and Newer Antimicrobial Drugs by Using the Radiometric BACTEC 460 Technique and the Proportion Method with Solid Media." Journal of Clinical Microbiology 37, no. 10 (1999): 3179–86. http://dx.doi.org/10.1128/jcm.37.10.3179-3186.1999.

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In a large multicenter study involving six major study sites in the United States, Canada, and Europe, the susceptibilities of 272Mycobacterium tuberculosis strains to classical second-line antituberculosis (anti-TB) drugs (capreomycin, cycloserine, ethionamide, and kanamycin) and newer compounds (amikacin, clofazimine, ofloxacin, and rifabutin) were determined by the radiometric BACTEC 460 procedure and the conventional proportion method on Middlebrook 7H10 agar. Previously established critical concentrations for classical second-line anti-TB drugs were compared with several concentrations in
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Ziotkowski, J., E. Kopeć, R. Linke-Koziotek, Z. Zwolska-Kwiek, and K. Ceglecka-Tomaszewska. "457-PA10 Bactec 460 TB (Becton-Dickinson) microbiological diagnostic in childhood tuberculosis." Tubercle and Lung Disease 76 (October 1995): 26. http://dx.doi.org/10.1016/0962-8479(95)90113-2.

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LOVE, GORDON L. "Cryptococcemia and the BACTEC 460 Blood Culture System." Annals of Internal Medicine 106, no. 4 (1987): 633. http://dx.doi.org/10.7326/0003-4819-106-4-633_2.

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Payaslıoğlu, Melda, İmran Sağlık, and Cüneyt Özakın. "Resistance Rates of Mycobacterium tuberculosis Complex Strains: A Retrospective Study in Türkiye." Medicina 61, no. 6 (2025): 1060. https://doi.org/10.3390/medicina61061060.

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Background and Objectives: Tuberculosis (TB) is one of the most common infectious diseases in developing countries. The resistance of the causative agent, Mycobacterium tuberculosis, to two or more first-line anti-TB drugs results in multidrug-resistant (MDR) TB, posing a serious challenge to the control of TB worldwide. This study was designed to determine the changes in drug resistance over time in TB strains isolated from patients in all departments of Uludağ University Hospital in western Türkiye. Materials and Methods: We retrospectively analyzed 104,598 clinical samples sent to our labor
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Dalton, Maurice Terence, and Eva Prevost. "CAPD Fluid Examination using the Bactec 460." Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis 6, no. 1 (1986): 9–11. http://dx.doi.org/10.1177/089686088600600104.

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In the microbiological laboratory CAPD fluid analysis is an unstandardized and sometimes a labor intensive procedure. This pilot study was set up to demonstrate the value of in-place technology -the Bactec 460 blood culture system, in reducing the work-load. Our results indicate that it is at least as effective as other, more tedious conventional methods. Since its introduction, continuous ambulatory peritoneal dialysis (CAPD) has proved to be a major advance in the management of end-stage renal disease (I). Despite refinements in technique over the years leading to more widespread use of CAPD
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Zhanel, George G., Marilyn H. Saunders, Joyce N. Wolfe, Daryl J. Hoban, James A. Karlowsky, and Amin M. Kabani. "Comparison of CO2 Generation (BACTEC) and Viable-Count Methods To Determine the Postantibiotic Effect of Antimycobacterial Agents against Mycobacterium avium Complex." Antimicrobial Agents and Chemotherapy 42, no. 1 (1998): 184–87. http://dx.doi.org/10.1128/aac.42.1.184.

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ABSTRACT The postantibiotic effects (PAEs) of antimycobacterial agents determined with a BACTEC TB-460 instrument (CO2 production) and by a traditional viable-count method against Mycobacterium avium complex (MAC) were not significantly different (P > 0.05). The longest PAEs following a 2-h exposure to 2× the MIC were induced by amikacin (10.3 h), rifampin (9.7 h), and rifabutin (9.5 h), while the shortest PAEs resulted from clofazimine (1.7 h) and ethambutol (1.1 h) exposure. CO2 generation is a valid and efficient means of determining in vitro PAEs against MAC.
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Cruciani, M., C. Scarparo, M. Malena, O. Bosco, G. Serpelloni, and C. Mengoli. "Meta-Analysis of BACTEC MGIT 960 and BACTEC 460 TB, with or without Solid Media, for Detection of Mycobacteria." Journal of Clinical Microbiology 42, no. 5 (2004): 2321–25. http://dx.doi.org/10.1128/jcm.42.5.2321-2325.2004.

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Das, Subrata, Ritu Mahata, and Amrita Gupta. "Evaluation of culture, molecular assay (TB-PCR), GeneXpert system and histopathology for detection of genital tuberculosis." Panacea Journal of Medical Sciences 12, no. 3 (2022): 615–21. http://dx.doi.org/10.18231/j.pjms.2022.115.

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Female genital tuberculosis (FGTB) has a crucial role in many gynaecological complaint and delay in diagnosis can cause irreversible damages. The new diagnostic technique GeneXpert system is accurate and rapid test can make paradigm shift in FGTB treatment. The aim of the study to estimate efficacy of different tests i.e. AFB BACTEC culture, molecular assay (TB-PCR) and histopathology in comparison to GeneXpert system to detect FGTB. The prospective observational study was conducted in between January, 2018 and June, 2019. Randomly selected total 62 patients were put under all four diagnostic
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Collins, L., and S. G. Franzblau. "Microplate alamar blue assay versus BACTEC 460 system for high-throughput screening of compounds against Mycobacterium tuberculosis and Mycobacterium avium." Antimicrobial Agents and Chemotherapy 41, no. 5 (1997): 1004–9. http://dx.doi.org/10.1128/aac.41.5.1004.

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In response to the need for rapid, inexpensive, high-throughput assays for antimycobacterial drug screening, a microplate-based assay which uses Alamar blue reagent for determination of growth was evaluated. MICs of 30 antimicrobial agents against Mycobacterium tuberculosis H37Rv, M. tuberculosis H37Ra, and Mycobacterium avium were determined in the microplate Alamar blue assay (MABA) with both visual and fluorometric readings and compared to MICs determined in the BACTEC 460 system. For all three mycobacterial strains, there was < or = 1 dilution difference between MABA and BACTEC median M
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Collins, L. A., M. N. Torrero, and S. G. Franzblau. "Green Fluorescent Protein Reporter Microplate Assay for High-Throughput Screening of Compounds againstMycobacterium tuberculosis." Antimicrobial Agents and Chemotherapy 42, no. 2 (1998): 344–47. http://dx.doi.org/10.1128/aac.42.2.344.

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ABSTRACT An optimal assay for high-throughput screening for new antituberculosis agents would combine the microplate format and low cost of firefly luciferase reporter assays and redox dyes with the ease of kinetic monitoring inherent in the BACTEC system. The green fluorescent protein (GFP) of the jellyfish Aequorea victoria is a useful reporter molecule which requires neither substrates nor cofactors due to the intrinsically fluorescent nature of the protein. The gene encoding a red-shifted, higher-intensity GFP variant was introduced by electroporation into Mycobacterium tuberculosis H37Ra
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Umpeleva, T. V., E. A. Mazurina, D. V. Vakhrusheva, and N. I. Eremeeva. "Comparison of Different Methods for Drug Susceptibility Testing of Mycobacterium tuberculosis to Rifampicin." Tuberculosis and Lung Diseases 100, no. 1 (2022): 41–48. http://dx.doi.org/10.21292/2075-1230-2022-100-1-41-48.

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The objective: to compare results of drug susceptibility testing to rifampicin by molecular genetic methods and phenotypic tests of Mycobacterium tuberculosis isolates obtained from clinical specimens of tuberculosis patients.Subjects and Methods. 915 samples of M. tuberculosis DNA and 426 cultures were used in this study. Genotypic tests (TB-TEST (BIOCIP-IMB, Russia), GenoType MTBDRplusV2) and phenotypic technologies (absolute concentration method, Bactec MGIT 960 system, Sensititre Myco TB kit) were used.Results. A high percentage (98.7%; CI 97.7-99.7%) of confirmation of the results of the
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Sriram, D., K. Jyothi Mallika, and P. Yogeeswari. "Synthesis of Tetrahydro-2H-[1,3,5]thiadiazine-5-(4-pyridylcarboxamido)-2-thione with antitubercular activity." Scientia Pharmaceutica 72, no. 1 (2004): 35–41. http://dx.doi.org/10.3797/scipharm.aut-04-04.

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3-Substituted-5-(4-pyridylcarboxamide)tetrahydro-2H-[1,3,5]thiadizine-2-thione derivatives (1-9) were synthesized as derivatives of isoniazid (INH) to overcome the resistance developed with its therapeutic use. The structures were confirmed by their spectral and elemental analyses data. These derivatives revealed higher lipophilicity compared with INH. The antimycobacterial activity of the synthesized compounds and INH was evaluated in vitro against Mycobacterium tuberculosis H37Rv at 6.25 µg/ml in BACTEC 12B medium using the BACTEC 460 radiometric system. The derivatives exhibited antitubercu
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Scarparo, Claudio, Paola Piccoli, Alessandra Rigon, Giuliana Ruggiero, Paolo Ricordi, and Claudio Piersimoni. "Evaluation of the BACTEC MGIT 960 in comparison with BACTEC 460 TB for detection and recovery of mycobacteria from clinical specimens." Diagnostic Microbiology and Infectious Disease 44, no. 2 (2002): 157–61. http://dx.doi.org/10.1016/s0732-8893(02)00437-6.

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Franzblau, Scott G., Richard S. Witzig, James C. McLaughlin, et al. "Rapid, Low-Technology MIC Determination with Clinical Mycobacterium tuberculosis Isolates by Using the Microplate Alamar Blue Assay." Journal of Clinical Microbiology 36, no. 2 (1998): 362–66. http://dx.doi.org/10.1128/jcm.36.2.362-366.1998.

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A colorimetric, microplate-based Alamar Blue assay (MABA) method was used to determine the MICs of isoniazid (INH), rifampin, streptomycin (SM), and ethambutol (EMB) for 34 PeruvianMycobacterium tuberculosis isolates (including both pansensitive and multidrug-resistant strains) and the H37Rv strain by using bacterial suspensions prepared directly from solid media. Results for all isolates were available within 8 days. Discordant results were observed on initial tests for 3 of 16 INH-susceptible isolates, 5 of 31 EMB-susceptible isolates, and 2 of 4 SM-resistant isolates (by the BACTEC 460 syst
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