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1

Okabe, Satoshi, Tomonori Kindaichi, and Tsukasa Ito. "Fate of 14C-Labeled Microbial Products Derived from Nitrifying Bacteria in Autotrophic Nitrifying Biofilms." Applied and Environmental Microbiology 71, no. 7 (July 2005): 3987–94. http://dx.doi.org/10.1128/aem.71.7.3987-3994.2005.

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ABSTRACT The cross-feeding of microbial products derived from 14C-labeled nitrifying bacteria to heterotrophic bacteria coexisting in an autotrophic nitrifying biofilm was quantitatively analyzed by using microautoradiography combined with fluorescence in situ hybridization (MAR-FISH). After only nitrifying bacteria were labeled with [14C]bicarbonate, biofilm samples were incubated with and without NH4 + as a sole energy source for 10 days. The transfer of 14C originally incorporated into nitrifying bacterial cells to heterotrophic bacteria was monitored with time by using MAR-FISH. The MAR-FI
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2

Ikuta, H., N. Noda, Y. Ebie, A. Hirata, S. Tsuneda, M. Matsumura, and Y. Inamori. "The rapid quantification and detection of nitrifying bacteria by using monoclonal antibody method." Water Science and Technology 42, no. 3-4 (August 1, 2000): 1–7. http://dx.doi.org/10.2166/wst.2000.0351.

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Monoclonal antibodies against the two kinds of nitrifying bacteria Nitrosomonas europaea (IFO14298) and Nitrobacter winogradskyi (IFO14297) were raised and isotypes of these monoclonal antibodies, IgM and IgG1, were successfully obtained. Cross reactivities of these monoclonal antibodies against various kinds of representative heterotrophic bacteria turned out to be relatively low by competitive ELISA. In contrast, these monoclonal antibodies were very specific for nitrifying bacteria used as antigens. By means of sandwich ELISA using different isotype monoclonal antibodies such as IgM and IgG
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3

Ayiti, Oluwatobi Esther, Ayansina Segun Ayangbenro, and Olubukola Oluranti Babalola. "16S Amplicon Sequencing of Nitrifying Bacteria and Archaea Inhabiting Maize Rhizosphere and the Influencing Environmental Factors." Agriculture 12, no. 9 (August 28, 2022): 1328. http://dx.doi.org/10.3390/agriculture12091328.

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Nitrifying bacteria and archaea are ubiquitous and can transform ammonia locked up in soil or manure into nitrate, a more soluble form of nitrogen. However, nitrifying bacteria and archaea inhabiting maize rhizosphere have not been fully explored. This study evaluates the diversity and abundance of nitrifying bacteria and archaea across different growth stages of maize using 16S amplicon sequencing. Moreover, the influence of environmental factors (soil physical and chemical properties) on the nitrifying communities was evaluated. Rhizosphere soil DNA was extracted using Nucleospin Soil DNA ex
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4

Inamori, Yuhei, Tomotake Takai, Naohiro Noda, Akira Hirata, Hiroshi Niioka, Gao YueHua, and Masatoshi Matsumura. "Development of a rapid quantification method for nitrosomonas and nitrobacter using elisa for wastewater treatment facilities." Water Science and Technology 36, no. 12 (December 1, 1997): 169–74. http://dx.doi.org/10.2166/wst.1997.0444.

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Enzyme-linked immunosorbent assay (ELISA) by use of monoclonal antibodies (MAbs) is very useful and helpful for the detection and quantification of the specific bacteria like nitrifiers in a mixed bacterial habitat. In this study, seven monoclonal antibodies were raised from splenocytes of mice(BALB/c) that are specific for the surface antigen of the two kinds of nitrifying bacteria. Three were directed against Nitrosomonas europaea (IFO 14298) and four were directed against Nitrobacter winogradskyi (IFO 14297). Cross-reactivities of MAbs against other strains of nitrifying bacteria as well as
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5

Alfisah, R. K., I. Rusmana, T. Widiyanto, and R. Affandi. "The Abundance and Potential Activity of Nitrifying, Denitrifying, and Nitrate-ammonifying Bacteria in the Vanamae Shrimp Culture in Karawang." IOP Conference Series: Earth and Environmental Science 1062, no. 1 (July 1, 2022): 012011. http://dx.doi.org/10.1088/1755-1315/1062/1/012011.

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Abstract The existence of inorganic nitrogen in the shrimp pond ecosystem will not be separated from the nitrogen cycle and microbiological processes including the activity of microbes. This study aimed to analyze the abundance and potential rate of nitrifying, denitrifying, and nitrate-ammonifying bacteria in Vanamae shrimp cultivation. Water samples were collected on a shrimp pond in Karawang, West Java. Water sampling was carried out at the age of shrimp rearing 0 days, 21 days, 65 days, and 89 days. Water sampling was conducted at four points representing an area of the pond. The bacterial
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6

Okabe, S., T. Kindaichi, Y. Nakamura, and T. Ito. "Eco-physiology of autotrophic nitrifying biofilms." Water Science and Technology 52, no. 7 (October 1, 2005): 225–32. http://dx.doi.org/10.2166/wst.2005.0205.

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Microautoradiography combined with fluorescent in situ hybridization (MAR-FISH), a powerful tool for linking physiology with identification of individual cells, was applied to investigate microbial interactions between nitrifying bacteria and coexisting heterotrophic bacteria in an autotrophic nitrifying biofilm community fed with only ammonia as the sole energy source and bicarbonate as the sole carbon source. First, nitrifying bacteria were radiolabeled by culturing the biofilm samples with [14C]bicarbonate for 6 h, and then the transfer of radioactivity from nitrifying bacteria to heterotro
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7

Han, Dengfeng, Zhenyi Hu, Dapeng Li, and Rong Tang. "Nitrogen Removal of Water and Sediment in Grass Carp Aquaculture Ponds by Mixed Nitrifying and Denitrifying Bacteria and Its Effects on Bacterial Community." Water 14, no. 12 (June 9, 2022): 1855. http://dx.doi.org/10.3390/w14121855.

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Nitrification and denitrification are important for nitrogen (N) cycling in fish ponds culture, but the effects of nitrifying and denitrifying bacteria concentrations on pond water and sediments remain largely unknown. Here, we used 0, 0.15, 0.30, 0.60 mg/L different concentrations of mixed nitrifying and denitrifying bacteria to repair the pond substrate through an enclosure experiment lasting 15 days. The results showed that the purification effect of nitrifying and denitrifying bacteria was most obvious on pond nitrogen from day 4 to day 7. The optimal relative concentration was 0.60 mg/L f
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8

Lai, Zi Ni, Ying De Cui, Peng Gao, and Xun Jun Chen. "Modified PLA Carrier Material and its Performance in Immobilization of Nitrifying Bacteria." Materials Science Forum 610-613 (January 2009): 198–201. http://dx.doi.org/10.4028/www.scientific.net/msf.610-613.198.

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To prepare the renewable carrier materials for immobilization of nitrifying bacteria, polylactic acid (PLA) dichloromethane solution was added to chitosan aqueous solution, mixed by agitation at a speed of 150 rpm / min. The resultant PLA microspheres were fund to have diameter of 100 ~ 300 μm, thus underwent ammonolysis by a 6 % hexamethylenediamine / n-propanol solution for 8 min, hydroformylation by a 1% glutaraldehyde solution for 3 h, and grafted with 1% chitosan for 24 h, to improve the surface hydrophilic property. The static adsorption was applied for adhesion of nitrifying bacteria to
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9

Yosmaniar, Y., T. Sumiati, and M. Mulyasari. "Growth Performance and Survival Rate of Catfish (Pangasius sp) with the Application of the Nitrifying and Denitrifying Bacteria." IOP Conference Series: Earth and Environmental Science 934, no. 1 (November 1, 2021): 012004. http://dx.doi.org/10.1088/1755-1315/934/1/012004.

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Abstract Nitrifying and denitrifying bacteria can be used as a bioremediation agents in aquaculture. The purpose of this experiment is to evaluate the optimal growth and survival performances of catfish rearing with the application of nitrifying and denitrifying bacteria. A completely randomized design was performed with the following treatments: A) nitrifying and denitrifying bacteria NP2-DP1; B) nitrifying and denitrifying bacteria NP2-DP2; C) commercial bacteria and D) without bacterial isolate (control), each with 3 replications. Twelve containers (34 x 34 x 45 cm) were used with a volume
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10

Sheng, Xiaolin, Rui Liu, Lujun Chen, Zihua Yin, and Jianfeng Zhu. "Enrichment and application of nitrifying activated sludge in membrane bioreactors." Water Science and Technology 76, no. 11 (August 14, 2017): 2888–94. http://dx.doi.org/10.2166/wst.2017.421.

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Abstract In this study, nitrifying bacteria were enriched in a membrane bioreactor (MBR, R1) and their bioaugmentation effectiveness was evaluated in another two MBRs (R2 and R3). Nitrifying activated sludge (NAS) with high nitrification activity of up to 3,000 mg-N/(L·d)−1 was successfully enriched in R1. The results showed that chemical oxygen demand concentration of 100–200 mg/L had no negative effect on NAS enrichment but reduced the ratio of bacterial nitrifiers. Moreover, the cell concentration of nitrifying bacteria in NAS, which was 3.1 × 1011 cells/L, was similar to that of the commer
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11

Bai, Xue, Haixin Gu, and Yulong Li. "Coimmobilized Microalgae and Nitrifying Bacteria for Ammonium Removal." International Journal of Environmental Science and Development 7, no. 6 (2016): 406–9. http://dx.doi.org/10.7763/ijesd.2016.v7.809.

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12

Schramm, Andreas, Dirk de Beer, Johan C. van den Heuvel, Simon Ottengraf, and Rudolf Amann. "Microscale Distribution of Populations and Activities ofNitrosospira and Nitrospira spp. along a Macroscale Gradient in a Nitrifying Bioreactor: Quantification by In Situ Hybridization and the Use of Microsensors." Applied and Environmental Microbiology 65, no. 8 (August 1, 1999): 3690–96. http://dx.doi.org/10.1128/aem.65.8.3690-3696.1999.

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ABSTRACT The change of activity and abundance of Nitrosospiraand Nitrospira spp. along a bulk water gradient in a nitrifying fluidized bed reactor was analyzed by a combination of microsensor measurements and fluorescence in situ hybridization. Nitrifying bacteria were immobilized in bacterial aggregates that remained in fixed positions within the reactor column due to the flow regimen. Nitrification occurred in a narrow zone of 100 to 150 μm on the surface of these aggregates, the same layer that contained an extremely dense community of nitrifying bacteria. The central part of the aggregates
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13

Bourgeois, François-René, Frédéric Monette, and Daniel G. Cyr. "Operational modifications for the development of nitrifying bacteria in a large-scale biological aerated filter and its impact on wastewater treatment." Water Science and Technology 78, no. 8 (October 25, 2018): 1704–14. http://dx.doi.org/10.2166/wst.2018.447.

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Abstract To develop a better understanding for fixed biomass processes, the development of a nitrifying bacterial biofilm, as well as the performance of treatment during modifications to operational conditions of a full-scale submerged biological filter were examined. The development of the nitrifying biofilm was investigated at four depth levels (1, 2, 4 and 5 feet). The result of bacterial subpopulations analyzed by qPCR relative to the physico-chemical parameters of the wastewater during the various tests (sustained aeration, modified backwash parameters and inflow restriction) revealed an
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14

Abeliovich, Aharon. "Nitrifying Bacteria in Wastewater Reservoirs." Applied and Environmental Microbiology 53, no. 4 (1987): 754–60. http://dx.doi.org/10.1128/aem.53.4.754-760.1987.

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15

Hill, Dennis R., and Terry Webster. "High pH Inhibits Nitrifying Bacteria." Opflow 34, no. 7 (July 2008): 20–22. http://dx.doi.org/10.1002/j.1551-8701.2008.tb02000.x.

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16

Cébron, Aurélie, Thierry Berthe, and Josette Garnier. "Nitrification and Nitrifying Bacteria in the Lower Seine River and Estuary (France)." Applied and Environmental Microbiology 69, no. 12 (December 2003): 7091–100. http://dx.doi.org/10.1128/aem.69.12.7091-7100.2003.

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ABSTRACT The Achères wastewater treatment plant, located just downstream of Paris, discharges its effluents into the lower Seine River. The effluents contain large numbers of heterotrophic bacteria, organic matter, and ammonium and are a source of nitrifying bacteria. As a result, degradation of organic matter by heterotrophic bacteria and subsequent oxygen depletion occur immediately downstream of the effluent outlet, whereas nitrifying bacteria apparently need to build up a significant biomass before ammonium oxidation significantly depletes the oxygen. We quantified the potential total nit
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17

Tsuneda, S., Y. Ejiri, T. Nagano, and A. Hirata. "Formation mechanism of nitrifying granules observed in an aerobic upflow fluidized bed (AUFB) reactor." Water Science and Technology 49, no. 11-12 (June 1, 2004): 27–34. http://dx.doi.org/10.2166/wst.2004.0796.

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The influences of trace metals in the wastewater and shear stress by aeration were particularly examined to clarify the formation mechanism of nitrifying granules in an aerobic upflow fluidized bed (AUFB) reactor. It was found that Fe added as a trace element to the inorganic wastewater accumulated at the central part of the nitrifying granules. Another result obtained was that suitable shear stress by moderate aeration (0.07-0.20 L/min/L-bed) promoted granulation. Furthermore, it was successfully demonstrated that pre-aggregation of seed sludge using hematite promoted core formation, leading
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18

Ding, Yuan Hong, Qing Wang, Hong Qiang Ren, and Jian Lu. "Effects of Trichloroethylene on the Wastewater Treatment in Membrane Bioreactors." Advanced Materials Research 588-589 (November 2012): 34–38. http://dx.doi.org/10.4028/www.scientific.net/amr.588-589.34.

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The activities of nitrifying bacteria and organic utilizing bacteria against TCE in sludge was investigated using three series of Membrane bioreactors, and the results indicated that, the removal efficiencies of COD decreased gradually, but was not affected severely with TCE inhibition, good organics removal efficiencies was possibly realized, while the ammonia removal efficiencies dropped sharply due to the severe inhibition of TCE against nitrifying bacteria, the degree of TCE inhibition against nitrifying bacteria increased with the TCE concentration, but low-concentration TCE addition seem
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19

Kindaichi, Tomonori, Tsukasa Ito, and Satoshi Okabe. "Ecophysiological Interaction between Nitrifying Bacteria and Heterotrophic Bacteria in Autotrophic Nitrifying Biofilms as Determined by Microautoradiography-Fluorescence In Situ Hybridization." Applied and Environmental Microbiology 70, no. 3 (March 2004): 1641–50. http://dx.doi.org/10.1128/aem.70.3.1641-1650.2004.

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ABSTRACT Ecophysiological interactions between the community members (i.e., nitrifiers and heterotrophic bacteria) in a carbon-limited autotrophic nitrifying biofilm fed only NH4 + as an energy source were investigated by using a full-cycle 16S rRNA approach followed by microautoradiography (MAR)-fluorescence in situ hybridization (FISH). Phylogenetic differentiation (identification) of heterotrophic bacteria was performed by 16S rRNA gene sequence analysis, and FISH probes were designed to determine the community structure and the spatial organization (i.e., niche differentiation) in the biof
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20

Okabe, S., and Y. Watanabe. "Structure and function of nitrifying biofilms as determined by in situ hybridization and the use of microelectrodes." Water Science and Technology 42, no. 12 (December 1, 2000): 21–32. http://dx.doi.org/10.2166/wst.2000.0232.

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Time dependent development of the spatial organization of NH4+- and NO2−-oxidizing bacterial populations in a domestic wastewater biofilm and in an autotrophic nitrifying biofilm were investigated by fluorescent in situ hybridization (FISH) with a set of 16S rRNA-targeted oligonucleotide probes. Population dynamics of nitrifying bacteria in the biofilms were correlated with the biofilm performance. In situ hybridization indicated that Nitrosomonas spp. (excluding probe NEU stained NH4+-oxidizing bacteria: i.e., N. marina-lineage, N. europaea-lineage, N. eutropha, and N. halophila) and Nitrospi
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21

Wang, Zhu, Bo Liu, and Ai Min Li. "Effects of Adding Nitrifying Bacteria on Microbial Communities and Nitrification in a Laboratory-Scale a/O Reactor Treating Leather-Tanning Wastewater." Advanced Materials Research 599 (November 2012): 289–94. http://dx.doi.org/10.4028/www.scientific.net/amr.599.289.

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A laboratory-scale anoxic/oxic reactor was used to analyze the effects of adding nitrifying bacteria on microbial communities in the treatment of leather-tanning wastewater. The reactor was operated in series in continuous flow mode for 25 d after an acclimation period of 45 d, and the nitrifying bacteria were added after the acclimation period. The addition of nitrifying bacteria into the wastewater significantly enhanced NH4+-N removal efficiency. The Arrhenius and Jacob-Monod models were applied to determine the optimum and acceptable operating conditions for this process. The optimum tempe
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22

Daims, H., P. H. Nielsen, J. L. Nielsen, S. Juretschko, and M. Wagner. "Novel Nitrospira-like bacteria as dominant nitrite-oxidizers in biofilms from wastewater treatment plants: diversity and in situ physiology." Water Science and Technology 41, no. 4-5 (February 1, 2000): 85–90. http://dx.doi.org/10.2166/wst.2000.0430.

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The frequency and distribution of putatively nitrite-oxidizing, Nitrospira- like bacteria in nitrifying biofilms from two reactors receiving wastewater with different ammonia and salt concentrations were observed by fluorescent in situ hybridization. For this purpose, new 16S rRNA-directed oligonucleotide probes targeting the bacterial phylum Nitrospira and the three main lineages within this phylum were developed and evaluated. The diversity of Nitrospira-like bacteria in the reactors was additionally investigated by retrieval and comparative analysis of full 16S rRNA sequences from the biofi
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23

Dong, Ziyi, Changhao Xiao, Weihua Zeng та Jinbo Zhao. "Impact of 17β-Estradiol on Natural Water’s Heterotrophic Nitrifying Bacteria". International Journal of Environmental Science and Development 12, № 1 (2021): 17–22. http://dx.doi.org/10.18178/ijesd.2021.12.1.1312.

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In this research, bottom water samples were collected from nature water. After cultivating and selecting, bacteria which could use (NH4)2SO4 as the only nitrogen source had been selected. The bacteria in different cultures with different concentration of 17β-estradiol (E2) were cultivated, and every group’s concentration of N-NH4 +, N-NO3 - and OD600 were measured. The result shows that compare with the control group, in which no E2 was added, the growth of heterotrophic nitrifying bacteria had been promoted when the concentration of E2 was in range of 1-100 ng/L. In addition, heterotrophic ni
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24

Hasebe, Yoshiaki, Hiroaki Meguro, Yuuki Kanai, Masahiro Eguchi, Toshifumi Osaka, and Satoshi Tsuneda. "High-rate nitrification of electronic industry wastewater by using nitrifying granules." Water Science and Technology 76, no. 11 (September 7, 2017): 3171–80. http://dx.doi.org/10.2166/wst.2017.431.

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Abstract Nitrifying granules have a high sedimentation property and an ability to maintain a large amount of nitrifying bacteria in a reaction tank. Our group has examined the formation process of nitrifying granules and achieved high-rate nitrification for an inorganic synthetic wastewater using these granules. In this research, a pilot-scale test plant with an 850-liter reaction tank was assembled in a semiconductor manufacturing factory in order to conduct a continuous water conduction test using real electronics industry wastewater. The aim was to observe the formation of nitrifying granul
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25

Aswiyanti, I., I. Istiqomah, and A. Isnansetyo. "Isolation and identification of nitrifying bacteria from tilapia (Oreochromis sp.) pond in Sleman Yogyakarta Indonesia." IOP Conference Series: Earth and Environmental Science 919, no. 1 (November 1, 2021): 012054. http://dx.doi.org/10.1088/1755-1315/919/1/012054.

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Abstract This research aims to isolate and identify autochtonous nitrifying bacteria from tilapia pond in Sleman Yogyakarta Indonesia for future application in aquaculture practices in the region. Bacteria were isolated using a nitrification medium. Bacterial characterization was carried out by non-pathogenic test to tilapia (Oreochromis sp.), and nitrification activity test in a single bacterial fermentation medium for 9 days. Bacterial identification was carried out based on the colony and cell morphologies, biochemical tests, and molecular analysis using the 16S rRNA and gyrB genes. A total
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26

Rodríguez Rodríguez, Abad, Silvia Mau Inchaustegui, Lilliana Piedra Castro, Ricardo Jiménez Montealegre, and Juan Pablo Herrera Vargas. "Isolation of ammonium- and nitrite-oxidizing bacterial strains from soil, and their potential use in the reduction of nitrogen in household waste water." Revista de Biología Tropical 65, no. 4 (September 19, 2017): 1527. http://dx.doi.org/10.15517/rbt.v65i4.26509.

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Currently, nitrogen has become the main element of water pollution, causing riverine, lacustrine and coastal eutrophication. The continuous contamination of aquifers and the absence of planned water resource utilization, boost its scarcity, and has been the only way in which our societies become aware of the urgent need to process the generated wastewater. The objective of this research was to evaluate the nitrifying capacity of different autochthonous bacterial isolates from soils from nearby sources of domestic wastewater drainage. For this, bacteria were isolated from Pirro River, contamina
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27

Fdz-Polanco, F., S. Villaverde, and P. A. García. "Temperature effect on nitrifying bacteria activity in biofilters: activation and free ammonia inhibition." Water Science and Technology 30, no. 11 (December 1, 1994): 121–30. http://dx.doi.org/10.2166/wst.1994.0552.

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Nitrifying bacteria activity and concentrations depend on specific free ammonia concentration (ratio NH3/biomass), that is a function of temperature, pH, ammonium concentration and nitrifying biomass concentration. So, temperature is a key parameter in the nitrification process producing two opposite effects: bacteria activation and free ammonia inhibition. These phenomena are studied in an up-flow biological aerated filter (UBAF) settled by a nitrifying biofilm (measured as Volatile Attached Solids, VAS). The plug flow allows to disclosure of both effects, activation and inhibition. For Nitro
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28

Sirotkin, A. S., J. V. Kobeleva, and E. S. Gorshkova. "Bio-Augmentation of Nitrifying Microorganisms to Increase the Efficiency of Oxidation of Nitrogen Compounds during Wastewater Biofiltration." Biotekhnologiya 36, no. 2 (2020): 99–107. http://dx.doi.org/10.21519/0234-2758-2020-36-2-99-107.

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The efficiency of nitrifying bacteria bio-augmentation into biofilm microbiocenosis during 30-day continuous biofiltration of municipal wastewater model solution has been assessed. The laboratory setup consisted of two parallel operating biofilters, in one of which, after start-up period, cultures of ammonium-oxidizing and nitrite-oxidizing bacteria of the Nitrobacter genus were sequentially introduced. It was established that the bio-augmentation of ammonium-oxidizing bacteria into the biofilm microbiocenosis led to an increase in the efficiency of ammonium nitrogen removal by an average of 1
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29

Jancarkova, Ivana, Tove A. Larsen, and Willi Gujer. "Distribution of nitrifying bacteria in a shallow stream." Water Science and Technology 36, no. 8-9 (October 1, 1997): 161–66. http://dx.doi.org/10.2166/wst.1997.0660.

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A project investigating the dynamics of self-purification processes in a shallow stream is carried out. Effects of the concentration gradient due to the distance to the pollution source, of hydraulic conditions in the river bed and of storm floods on the distribution of nitrifying bacteria were studied with the help of laboratory and field experiments. Nitrifiers density on the surface of the stream bed increased rapidly up to a distance of 300 m from the WWTP indicating possible competition of the nitrifiers with the heterotrophic bacteria close to the WWTP. Afterwards a slight decrease in th
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30

Tarre, S., M. Beliavski, N. Denekamp, A. Gieseke, D. de Beer, and M. Green. "High nitrification rate at low pH in a fluidized bed reactor with chalk as the biofilm carrier." Water Science and Technology 49, no. 11-12 (June 1, 2004): 99–105. http://dx.doi.org/10.2166/wst.2004.0814.

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A typical steady state bulk pH of about 5 was established in a nitrifying fluidized bed with chalk as the only buffer agent. In spite of the low pH, high rate nitrification was observed with the nitrification kinetic parameters in the chalk reactor similar to those of biological reactors operating at pH>7. Various methods were used to determine the reasons for high rate nitrification at such low pH including (i) determination of bacterial species, (ii) microsensor measurements in the biofilm, and (iii) comparison of nitrification performance at low pH with a non-chalk fluidized bed reac
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31

Acevedo, Juan Diego, and Jenny Dussán. "Ammonium Cycling and Nitrification Stimulation during Oil Sludge Remediation by Gram-Positive Bacteria Lysinibacillus sphaericus Using Red Wiggler Earthworm Eisenia fetida." AgriEngineering 2, no. 4 (November 13, 2020): 544–55. http://dx.doi.org/10.3390/agriengineering2040036.

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The performance of a mixture between L. sphaericus and E. fetida was evaluated for ammonium cycling and nitrifying bacteria stimulation during oil sludge remediation. The addition of E. fetida significantly increased ammonium concentration (p = 0.0218) and total Colony-forming units (CFU) count p = 0.02848). However, oil sludge with worms and L. sphaericus reached lower ammonium concentrations and CFU counts than sludge with worms alone. Sludge inoculated only with L. sphaericus presented higher ammonium concentration than sludge without inoculum, but the bacterial population reached a lower d
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32

Okabe, Satoshi, Hisashi Satoh, and Yoshimasa Watanabe. "In Situ Analysis of Nitrifying Biofilms as Determined by In Situ Hybridization and the Use of Microelectrodes." Applied and Environmental Microbiology 65, no. 7 (July 1, 1999): 3182–91. http://dx.doi.org/10.1128/aem.65.7.3182-3191.1999.

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ABSTRACT We investigated the in situ spatial organization of ammonia-oxidizing and nitrite-oxidizing bacteria in domestic wastewater biofilms and autotrophic nitrifying biofilms by using microsensors and fluorescent in situ hybridization (FISH) performed with 16S rRNA-targeted oligonucleotide probes. The combination of these techniques made it possible to relate in situ microbial activity directly to the occurrence of nitrifying bacterial populations. In situ hybridization revealed that bacteria belonging to the genus Nitrosomonas were the numerically dominant ammonia-oxidizing bacteria in bot
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33

Gnida, Anna, Sebastian Żabczyński, and Joanna Surmacz-Górska. "Filamentous bacteria in the nitrifying activated sludge." Water Science and Technology 77, no. 11 (May 14, 2018): 2709–13. http://dx.doi.org/10.2166/wst.2018.215.

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Abstract Filamentous bacteria in addition to wastewater treatment are responsible for the shape of flocs and sedimentation properties of activated sludge. Their dynamics in activated sludge influences the performance of the whole sewage treatment plant. Therefore the composition of activated sludge biocenosis and its dynamics in the nitrification process were investigated. Four laboratory-scale activated sludge membrane bioreactors fed with wastewater highly concentrated with ammonium (synthetic wastewater imitating landfill leachate) were operated to obtain a high rate of nitrification. The s
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34

Umiyati, Uum. "Allelopathic Inhibition of Nitrifying Bacteria by Legumes." Journal of Tropical Soils 22, no. 2 (May 1, 2017): 125–30. http://dx.doi.org/10.5400/jts.2017.v22i2.125-130.

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The study aimed at understanding the competitive ability of legumes Vigna radiata L. and Mucuna pruriens with weeds and their effects on the activity of nitrifying bacteria in soils and the contents of organic-N in legumes and weeds. The experiment was arranged in a randomized block design with three factors and four replications. The first factor was soil order, i.e. Inceptisol and Vertisol; the second factor was types of legumes, i.e. Vigna radiata L. cultivar Sriti and Mucuna pruriens; and the third factor was weed management, i.e. with weed management and without weed management. The resul
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35

Lee, Yong-Woo, Say-Kee Ong, and Chikashi Sato. "Effects of heavy metals on nitrifying bacteria." Water Science and Technology 36, no. 12 (December 1, 1997): 69–74. http://dx.doi.org/10.2166/wst.1997.0432.

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Laboratory evaluations were conducted to study the toxic responses of heavy metals such as copper and nickel of an autotrophic culture of strict nitrifiers (Nitrosomonas sp. and Nitrobacter sp.) in continuous flow stirred tank reactors (CSTR). One of the CSTRs was operated as a suspended growth (SG) system while the other was operated as an attached & suspended growth (A&SG) system. Nitrification inhibition in a SG and A&SG systems was investigated with the emphasis on the effect of shock loading of copper and nickel. As a result of the copper and nickel test, Nitrosomonas sp. was
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36

Stein, Lisa Y., and Martin G. Klotz. "Nitrifying and denitrifying pathways of methanotrophic bacteria." Biochemical Society Transactions 39, no. 6 (November 21, 2011): 1826–31. http://dx.doi.org/10.1042/bst20110712.

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Nitrous oxide, a potent greenhouse gas and ozone-depleting molecule, continues to accumulate in the atmosphere as a product of anthropogenic activities and land-use change. Nitrogen oxides are intermediates of nitrification and denitrification and are released as terminal products under conditions such as high nitrogen load and low oxygen tension among other factors. The rapid completion and public availability of microbial genome sequences has revealed a high level of enzymatic redundancy in pathways terminating in nitrogen oxide metabolites, with few enzymes involved in returning nitrogen ox
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37

Naghdi, Mitra, Maximiliano Cledon, Satinder Kaur Brar, and Antonio Avalos Ramirez. "Nitrification of vegetable waste using nitrifying bacteria." Ecological Engineering 121 (October 2018): 83–88. http://dx.doi.org/10.1016/j.ecoleng.2017.07.003.

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Wang, Guangyu, Lei Chen, Fang Ma, Zhong Xu, and Weiguo Li. "Screening and identification of marine nitrifying bacteria." Journal of Biotechnology 136 (October 2008): S550. http://dx.doi.org/10.1016/j.jbiotec.2008.07.1293.

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39

Manser, R., W. Gujer, and H. Siegrist. "Membrane bioreactor versus conventional activated sludge system: population dynamics of nitrifiers." Water Science and Technology 52, no. 10-11 (November 1, 2005): 417–25. http://dx.doi.org/10.2166/wst.2005.0719.

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Although membrane bioreactors have attracted increasing attention in recent years, little research has been undertaken on the influence of the membrane separation on the microbial community composition. This paper compares the startup behaviour and the performance of the subsequent eight months of a membrane bioreactor with a conventional activated sludge pilot plant. Both plants were operated in parallel at the same sludge age and treated the same domestic wastewater. The identification of the nitrifying community composition using fluorescent in situ hybridization revealed only minor differe
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40

Jiao, Y., W. B. Jin, Q. L. Zhao, G. D. Zhang, Y. Yan, and J. Wan. "Transformation of nitrogen and distribution of nitrogen-related bacteria in a polluted urban stream." Water Science and Technology 60, no. 6 (September 1, 2009): 1597–605. http://dx.doi.org/10.2166/wst.2009.502.

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Most researchers focused on either nitrogen species or microbial community for polluted urban stream while ignoring the interaction between them and its effect on nitrogen transformation, which restricted the rational selection of an effective and feasible remediation technology. Taking Buji stream in Shenzhen (China) as target stream, the distribution of nitrogen-related bacteria was investigated by most probable number (MPN) besides analysis of nitrogen species etc. The nitrogen-related bacteria in sediment were 102 times richer than those in water. Owing to their faster growth, the MPN of a
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41

Hu, T. L., and K. T. Kung. "Study of heterotrophic nitrifying bacteria from wastewater treatment systems treating acrylonitrile, butadiene and styrene resin wastewater." Water Science and Technology 42, no. 3-4 (August 1, 2000): 315–21. http://dx.doi.org/10.2166/wst.2000.0397.

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In this study we investigated the population of heterotrophic nitrifying bacteria in three biological wastewater treatment systems treating acrylonitrile, butadiene and styrene (ABS) resin wastewater. In addition to isolating and identifying these bacteria, we examined the physiological properties of the isolates, particularly utilization of acrylonitrile and its derivatives. An enrichment process was employed to isolate heterotrophic nitrifying bacteria from sludge samples taken from three biological wastewater treatment systems. Thirteen isolates were obtained and four strains were identifie
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42

Tanaka, Kazuhiro, Minoru Tada, Takashi Kimata, Shouji Harada, Yuhko Fujii, Tamotu Mizuguchi, Naomichi Mori, and Hiroyoshi Emori. "Development of New Nitrogen Removal System Using Nitrifying Bacteria Immobilized in Synthetic Resin Pellets." Water Science and Technology 23, no. 4-6 (February 1, 1991): 681–90. http://dx.doi.org/10.2166/wst.1991.0518.

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A newly developed process applying a technique of microorganism immobilization to biological nitrogen removal is reported. Nitrifiers immobilized in 2 to 3 mm diameter polyethylene-glycol resin (Nitrifying pellets) are mixed with activated sludge in the nitrification tanks to promote quick nitrification. Due to the high nitrifying activity of the pellets, nitrification of municipal wastewaters is completed within a few hours. This paper introduces the synthetic polymer and immobilization method suited to nitrifiers, the conditions for pellet fluidization, the activity of nitrifying pellets and
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43

Xu, Jinlan, Haiyang Zhang, Rong Zhao, and Fanxing Kong. "Enhanced bacterial quorum aggregation on a zeolite capping layer for sustainable inhibition of ammonium release from contaminated sediment." Water Science and Technology 76, no. 12 (September 27, 2017): 3428–40. http://dx.doi.org/10.2166/wst.2017.507.

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Abstract The main objective of this study was to investigate how signal molecules enhance bacterial quorum aggregation on a zeolite capping layer for sustainable inhibition of ammonium release from contaminated sediment. Sediment remediation experiments were carried out by using nitrifying bacteria (WGX10, WGX18), denitrifying bacteria (HF3, HF7) and two kinds of signal molecules (OHHL, C8-HSL). The results showed that nitrifying bacteria and denitrifying bacteria could significantly aggregate on zeolite after adding 1.0 μM OHHL at a C/N ratio of 7. The maximum ammonium removal of five times t
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44

Ikuesan, Felix Adeleke, Iyanu Ehuwa, and Bartholomew Saanu Adeleke. "‘Petroleum derivatives’ toxicity: influence on the growth of soil nitrifying bacteria." Dutse Journal of Pure and Applied Sciences 8, no. 3b (October 14, 2022): 44–54. http://dx.doi.org/10.4314/dujopas.v8i3b.5.

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Over dependence on crude oil and its derivatives to drive our daily activities for energy and economy results in frequent discharge of petrochemicals into the environment causing pollution of varying magnitude with worrisome ecological and environmental impact of global concern. This study aimed to determine the toxicity of petroleum derivatives on soil nitrifying bacteria. Soil samples were collected into sterile polythene bag and transported to the laboratory for analyses. Microbiological analyses of the soil samples were performed using conventional and standard microbiological techniques.
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45

Yosmaniar, Yosmaniar, Hessy Novita, and Eri Setiadi. "ISOLASI DAN KARAKTERISASI BAKTERI NITRIFIKASI DAN DENITRIFIKASI SEBAGAI KANDIDAT PROBIOTIK." Jurnal Riset Akuakultur 12, no. 4 (January 18, 2018): 369. http://dx.doi.org/10.15578/jra.12.4.2017.369-378.

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Senyawa nitrogen yang tinggi pada limbah budidaya perikanan intensif dapat memperburuk kualitas air, sehingga perlu diatasi dengan penambahan probiotik untuk proses bioremediasi. Tujuan penelitian ini adalah untuk mendapatkan bakteri nitrifikasi dan denitrifikasi yang berpotensi sebagai kandidat probiotik pengendali senyawa nitrogen pada budidaya ikan air tawar. Tahap penelitian terdiri atas: 1) koleksi sampel air dan sedimen dari kolam budidaya ikan patin di kawasan minapolitan Desa Pudak Kecamatan Kumpeh Kabupaten Muaro Jambi Provinsi Jambi dan Desa Koto Mesjid Kecamatan XIII Koto Kampar Kab
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46

Placella, Sarah A., and Mary K. Firestone. "Transcriptional Response of Nitrifying Communities to Wetting of Dry Soil." Applied and Environmental Microbiology 79, no. 10 (March 22, 2013): 3294–302. http://dx.doi.org/10.1128/aem.00404-13.

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ABSTRACTThe first rainfall following a severe dry period provides an abrupt water potential change that is both an acute physiological stress and a defined stimulus for the reawakening of soil microbial communities. We followed the responses of indigenous communities of ammonia-oxidizing bacteria, ammonia-oxidizing archaea, and nitrite-oxidizing bacteria to the addition of water to laboratory incubations of soils taken from two California annual grasslands following a typically dry Mediterranean summer. By quantifying transcripts for a subunit of bacterial and archaeal ammonia monooxygenases (
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47

Tanaka, Y., K. Taguchi, and H. Utsumi. "Toxicity assessment of 255 chemicals to pure cultured nitrifying bacteria using biosensor." Water Science and Technology 46, no. 11-12 (December 1, 2002): 331–35. http://dx.doi.org/10.2166/wst.2002.0758.

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The bioassay has been attracting attention as a method of toxicity assessments of micropollutants in the environment. In this study, we report the characteristics (selectivity and sensitivity) of the nitrifying bacteria biosensor for 255 kinds of chemicals as a model of chemical contaminant in the environment and the results of evaluation of mixed samples of several substances. In the nitrifying bacteria respiration inhibition test using the biosensor, 56 chemicals were detected. It was found that this biosensor is especially sensitive to seven chemicals that have a thiocarbonyl functional gro
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48

Fu, Jinxiang, Zhe Zhang, and Jinghai Zhu. "Study on the diversity of denitrification bacteria treating with wastewater by using PPGC filler on SBMBBR at low temperature." E3S Web of Conferences 158 (2020): 04002. http://dx.doi.org/10.1051/e3sconf/202015804002.

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Aiming at the problem of the low removal efficiency of biological nitrogen-removing of low temperature waste-water, using Polyurethane Porous Gel Carrier (PPGC)-SBMBBR treated low temperature sewage, in compared with conventional SBR,and viaing Miseq high-throughput sequencing technology in analysis of the differences of microbial diversity and abundance of structure on the two reactors of activated sludge, revealed dominant nitrogen-removing bacterium improving the treatment efficiency of low temperature sewage. The results shows that the removal efficiency of the effluent nitrogen and the sl
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49

Zhang, Ji Ku, Hui Ting Shao, Yue Ming, and Hui Ye Wang. "Study on Biofortification in Vertical Flow Constructed Wetland." Advanced Materials Research 955-959 (June 2014): 2570–73. http://dx.doi.org/10.4028/www.scientific.net/amr.955-959.2570.

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The methods of dosing composite microbial inoculants are used in vertical flow constructed wetlands for biofortification. The removal of the main pollution indicators by substrate microorganisms was studied. The results show that compared with the blank system, the number of bacteria and nitrifying bacteria was higher in the substrate of constructed wetland by biofortification. At the hydraulic loading rate (HLR) of about 0.8 m3/(m2·d), relationship between total number of bacteria and the removal rate of COD was significant in Device A and Device B. Relationship with TP removal rate is not ev
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Prastowo, Bambang Widyo, Manja Meyky Bond, and Betutu Senggagau. "Perbandingan Sistem Resirkulasi dan Air Mengalir Untuk Pembesaran Lobster Pasir (Panulirus Homarus): Kajian Dinamika Kualitas Air." Barakuda 45: Jurnal Ilmu Perikanan dan Kelautan 4, no. 1 (April 28, 2022): 12–23. http://dx.doi.org/10.47685/barakuda45.v4i1.205.

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Aquaculture with the Recirculation Aquaculture System (RAS) is a unique engineered ecosystem that minimizes environmental disturbances by reducing the discharge of nutrient pollution through reuse of water used in fish farming. This activity was conducted at LP2IL Serang field test facility starting in June-November 2020. In this test, the RAS system used for growing of sand lobster uses the stages of purification: sedimentation, physical filtration, biological filtration, water disinfection with UV irradiation, removal of CO2, clean water reservoirs and sand lobster rearing tanks. Water quali
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