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Rozprawy doktorskie na temat „Bacteriophage”

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Data publikacji: 4 czerwca 2021
Data aktualizacji: 25 lipca 2025

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1

Cramer, Todd James Lucas. "Genetic mosaicism between the bacteriophage [phi]80 and bacteriophage [lambda]." Bowling Green, Ohio : Bowling Green State University, 2008. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=bgsu1223514067.

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2

Cramer, Todd James. "Genetic Mosaicism Between The Bacteriophage φ80 And Bacteriophage λ". Bowling Green State University / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1223514067.

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3

Ryan, Elizabeth Michelle. "Polymeric bacteriophage delivery systems." Thesis, Queen's University Belfast, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.558175.

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Phage therapy is the use of bacteriophages to treat bacterial infections. Once a prominent method of antibacterial therapy, phage therapy became almost forgotten following the discovery of antibiotics in 1928. The rising instance of antibiotic resistant bacteria in the last number of years has resulted in resurgence in interest in phage therapy. Research into delivery methods for bacteriophages has been very limited. Up to very recently, phages had only been delivered parentrally using crude and purified phage stocks and orally for gastrointestinal infections. In order for phage therapy to bec
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4

Thanki, Anisha M. "Development of a phage-based diagnostic test for the identification of Clostridium difficile." Thesis, Loughborough University, 2016. https://dspace.lboro.ac.uk/2134/20340.

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Clostridium difficile is the most common bacterial cause of infectious diarrhoea in healthcare environments and in 2014 was responsible for 13,785 infections in the UK. C. difficile infection (CDI) is spread via the faecal-oral route and by contact with contaminated surfaces. However, despite the healthcare concerns no tests are available to validate if sufficient cleaning has been conducted. In addition, Polymerase Chain Reaction (PCR) and Enzyme Immunoassays (EIAs)-based tests used to diagnose CDI lack sensitivity and specificity and hence false negative results are commonly obtained. To ove
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5

Terry, Tamsin Deborah. "Peptide display on filamentous bacteriophage." Thesis, University of Cambridge, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.627599.

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6

Nemavhulani, Shonisani. "Bacteriophage diversity in haloalkaline environments." University of the Western Cape, 2013. http://hdl.handle.net/11394/4313.

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>Magister Scientiae - MSc<br>There are limited reports on virus population in haloalkaline environments; therefore the aim of this study was to investigate the genetic diversity and biology of bacteriophage communities in these environments. Bacteria were isolated to be used as phage hosts. One bacterium from Lake Magadi and four bacteria from Lake Shala were successfully isolated from sediment samples. A further two Lake Shala bacterial hosts from the IMBM culture collection were also used to isolate bacteriophages. Bacterial isolates were identified to be most closely related to Bacil
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7

Abedon, Stephen Tobias. "The ecology of bacteriophage T4." Diss., The University of Arizona, 1990. http://hdl.handle.net/10150/185040.

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In this dissertation I explore the ecology of bacteriophage T4, a virus of Escherichia coli. In particular, I argue that the life history of bacteriophage T4 can be divided into the growth and survival of T4 phages in three distinct environments. I argue that these environments are distinguished by at least two T4 phage sensory systems. These include (i) the sensing of secondary adsorption by infecting phages and (ii) the determination of the concentration of monovalent cations and free tryptophan in solution about free T4 phage particles. The first environment consists of high concentrations
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8

Forghani, Farnaz. "Protein engineering of bacteriophage Mu transposase." Thesis, McGill University, 1990. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=60444.

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Bacteriophage Mu is an ideal system to study DNA transposition. The 70-KDa protein product of the phage early gene A, termed transposase, is absolutely required for transposition. Transposase binds specifically at sites located at both ends of the phage genome, termed attL and attR, and at an enhancer-like element at the left end of the genome, called IAS (internal activation sequence). It then nicks at these ends, and nicks a random target DNA sequence in a 5 base pair staggered fashion with 5$ sp prime$ extensions and promotes strand transfer between the Mu ends and the target DNA. The trans
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9

Long, Graham Stanley. "Molecular cloning of bacteriophage K1E endosialidase." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.339539.

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10

Sorrell, Julian Anthony. "An investigation into strong bacteriophage promoters." Thesis, Bangor University, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.361171.

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11

Toropova, Katerina. "Cryo-electron microscopy of bacteriophage MS2." Thesis, University of Leeds, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.503345.

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12

Alvarez, Ana Gonzalez. "Structural studies of filamentous bacteriophage proteins." Thesis, Keele University, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.332387.

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13

Huang, Haomin. "Studies on transduction by bacteriophage P1." Thesis, University of Edinburgh, 2003. http://hdl.handle.net/1842/14124.

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The original aim of this thesis was to address the origin of the abortive transduction protein (TPA), which appears to join the ends of transduced DNA and render it refractory to degradation by host nuclease and recombination with the chromosome. We planned to isolate abortively transduced DNA (ATD) from transduced cells, which should have TPA attached, in order to characterize it. To increase the packaging of transducing DNA and TPA, a <i>pac</i> site was inserted into the “donor” chromosome which was labelled with BU to make it heavy. This method proved technically difficult and I did not su
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14

OBRINGER, JOHN WILLIAM. "GENETIC EXCLUSION IN BACTERIOPHAGE-T4 (EXONUCLEASES)." Diss., The University of Arizona, 1987. http://hdl.handle.net/10150/184090.

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Genetic exclusion in phage T4 is the prime responsibility of the imm and sp genes. The map region containing imm does not allow sufficient bps to encode for proteins the size reported for the imm gp. After assaying 30 mutants of the genes adjacent to imm, I found 7 in gene 42 that were defective in the imm phenotype. Upon reverting amNG411(42), the mutant most defective exclusion, for its gene 42 phenotype the exclusion phenotype also changed. When assayed in UGA suppressor hosts, imm+ phage showed a decreased exclusion ability indicating that an opal codon was involved in production of the fu
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15

Smerk, Cari L. "P1 Bacteriophage and Tol System Mutants." Bowling Green State University / OhioLINK, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1182462962.

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16

Bhandare, Sudhakar Ganapati. "Biocontrol of V. cholerae using bacteriophage." Thesis, University of Nottingham, 2015. http://eprints.nottingham.ac.uk/29904/.

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Cholera is a persistent threat to public health and is endemic in many countries. Of late, there is an emergence of antibiotic resistance in Vibrio cholerae and treatment is effective only if given early, thus there is a need for rapid and more effective treatment of cholera. One such treatment could be the use of bacteriophages. During infection, V. cholerae adheres to the surface of enterocytes but does not invade the host. They are therefore not protected from bacteriophage infection. The study presented in this dissertation evaluates the potential of bacteriophage being used as a biocontro
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17

Jie, Zexin. "Engineering bacteriophage to overcome antibiotic resistance." Thesis, The University of Sydney, 2022. https://hdl.handle.net/2123/28877.

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Antimicrobial resistance is a global health issue that is endangering millions of lives and has been seen as a major threat to public health. Due to the rapid evolution of drug resistance, clinically available antibiotics are unsustainable, and thus, new antibiotics are urgently demanded to combat this crisis. Some novel antibacterial agents have been demonstrated as potential alternatives to replace commonly used antibiotics, but evidence has indicated that bacteria still can produce resistance to these antimicrobial agents. Thus, it is essential to develop new strategies for effectively cont
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18

Luan, Weisha. "Structural studies on bacteriophage portal proteins." Thesis, University of York, 2013. http://etheses.whiterose.ac.uk/4800/.

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In tailed bacteriophages and evolutionarily related herpes viruses, the portal protein is a central component of the DNA packaging molecular motor, which translocates viral genomic DNA into a preformed procapsid. The motor is the most powerful molecular machine discovered in nature, generating forces reaching ~50 pN and translocating DNA with a speed of several hundred bp/sec using ATP as an energy source. The oligomeric portal protein ring is situated at a unique vertex of the procapsid forming a conduit for DNA entry and exit. Although the three-dimensional structure has already been determi
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19

Marion, William R. "Bacteriophage P22 scaffolding protein functions and mechanisms in procapsid assembly /." Thesis, Birmingham, Ala. : University of Alabama at Birmingham, 2007. https://www.mhsl.uab.edu/dt/2009r/marion.pdf.

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20

Jakutyte, Lina. "Bacteriophage SPP1 entry into the host cell." Phd thesis, Université Paris Sud - Paris XI, 2011. http://tel.archives-ouvertes.fr/tel-00669654.

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The four main steps of bacterial viruses (bacteriophages) lytic infection are (i) specific recognition and genome entry into the host bacterium, (ii) replication of the viral genome, (iii) assembly of viral particles, and (iv) their release, leading in most cases to cell lysis. Although the course of individual steps of the viral infection cycle has been relatively well established, the details of how viral DNA transits from the virion to the host cytoplasm and of how the cellular environment catalyzes and possibly organizes the entire process remain poorly understood.Tailed bacteriophages are
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21

Grew, Edward Nicholas Delung. "Individual Based Modelling of Bacteria-Bacteriophage Interactions." Thesis, University of Exeter, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.486781.

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This thesis presents a novel individual based model capable of simulating the types of behaviour observed between bacteria and phage. Parasite-host relationships in bacterial ecology have commonly been modelled using a top-down differential ~quation approach tnat describes populations as single entities. These biological systems are intrinsically based on individuals whose behaviour relies on the interactions of many. A bottom-up, individualbased modelling approach has been used to simulate the behaviour observed between a bacterium and its corresponding bacteriophage. The use of individual ba
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22

Pieroni, Peter. "Application of bacteriophage typing to Klebsiella pneumoniae." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1996. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq23909.pdf.

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23

Korehei, Reza. "Encapsulation of T4 bacteriophage in electrospun biopolymers." Thesis, University of British Columbia, 2013. http://hdl.handle.net/2429/44398.

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Packaging foods with antibacterial electrospun fibrous mats, in particular the incorporation of bacteria specific viruses such as bacteriophages may address concerns triggered by recent waves of bacterial food contamination. To this end several methods for incorporating or encapsulating T4 bacteriophage into electrospun fibres were investigated. The incorporation of T4 bacteriophage using simple suspension electrospinning lead to major losses in T4 bacteriophage activity, with more than five-orders of magnitude decrease in activity being observed. Improved T4 bacteriophage viability was obtain
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24

Rooks, David John. "Molecular ecology of shiga-toxin encoding bacteriophage." Thesis, University of Liverpool, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.548790.

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25

Brockhurst, Michael. "The evolutionary ecology of bacteria-bacteriophage interactions." Thesis, University of Oxford, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.509898.

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26

Atterbury, Robert J. "Bacteriophage control of Campylobacters in retail poultry." Thesis, University of Nottingham, 2004. http://eprints.nottingham.ac.uk/14066/.

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Food-borne disease continues to be a major cause of human morbidity and mortality. During the past few decades, Campylobacter jejuni has ascended to become the greatest cause of bacterial enteric disease worldwide. Anecdotal evidence suggests the majority of human campylobacteriosis in industrialised countries is caused by the consumption of undercooked chicken. Campylobacter continues to frustrate current control strategies throughout the food chain and in 2001 was responsible for over 56, 000 cases of food poisoning in the U.K. alone. The work presented in this thesis examined the potential
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27

Green, Michael. "Studies on Bacillus bacteriophage populations in compost." Thesis, University of Liverpool, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307668.

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28

Loc-Carrillo, Catherine M. "Bacteriophage control of campylobacters in poultry production." Thesis, University of Nottingham, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.416725.

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29

Leggate, Daniel Richard. "Characterisation and mutagenesis of bacteriophage K1E endosialidase." Thesis, University of Cambridge, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.621894.

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30

Stocking, Kristin 1959. "Adsorption of MS-2 bacteriophage to silica." Thesis, The University of Arizona, 1989. http://hdl.handle.net/10150/277019.

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Batch and column experiments were performed to investigate the adsorption of bacteriophage MS-2 to silica at pH 5. Linear isotherm analysis of batch experiment data gave partition coefficient (KP) estimates of 270 cm³/g and 580 cm³/g for 4°C and 24°C, respectively. Breakthrough-type column experiments indicated that sorption and desorption were slow, as evidenced by a slow approach to breakthrough and tailing of the desorption limb. A non-equilibrium advection-dispersion model with all adsorption sites on the silica assumed to be kinetically controlled was used to model the column data. The mo
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31

Palasingam, Kampan. "Homologous Recombination in Q-Beta Rna Bacteriophage." Thesis, University of North Texas, 1992. https://digital.library.unt.edu/ark:/67531/metadc500683/.

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Q-Beta phage RNAs with inactivating insertion (8 base) or deletion (17 base) mutations within their replicase genes were transfected into Escherichia coli spheroplasts containing QB replicase provided in trans by a resident plasmid. Replicase-defective (Rep~) Q3 phage produced by these spheroplasts were unable to form plaques on cells lacking this plasmid. When individual Rep~ phage were isolated and grown to high titer in cells containing plasmid derived Q3 replicase, revertant Q3 phage (Rep'), with the original mutation (insertion or deletion) repaired, were obtained at a frequency of ca. 1
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32

Grayson, Paul Politzer David. "The DNA ejection process in bacteriophage lambda /." Diss., Pasadena, Calif. : California Institute of Technology, 2007. http://resolver.caltech.edu/CaltechETD:etd-05252007-103551.

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33

Kroeger, Thomas William 1952. "Hydrophobic partitioning of the bacteriophage MS-2." Thesis, The University of Arizona, 1989. http://hdl.handle.net/10150/276963.

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In batch experiments at pH's 5 and 7, the partitioning of MS-2 between water and silica (unbonded) was compared with the partitioning between water and silica with 6.5 percent of the surface covered by hydrophobic C18 chains (bonded). The roles of double-layer and van der Waals forces in partitioning were explored by modeling the potential energies of interaction. MS-2 adsorption to unbonded silica was negligible at pH 7, but did occur at pH 5. Adsorption was independent of pH with the bonded silica and approximately 2.6 orders of magnitude greater than the unbonded at pH 5, suggesting the imp
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34

Ranganathan, Srikanth. "Genetic and biochemical characterization of suppressors in the self-splicing nrdB intron of bacteriophage T4." Thesis, Georgia Institute of Technology, 1994. http://hdl.handle.net/1853/25333.

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35

Kwon, Heajoon Yim. "Genetic and biochemical studies of the self-splicing nrdB intron of bacterical virus T4." Diss., Georgia Institute of Technology, 1991. http://hdl.handle.net/1853/27591.

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36

Bedford, David Jonathan. "Molecular genetic analysis of the phage growth limitation (Pgl) system of Streptomyces coelicolor A3(2)." Thesis, University of East Anglia, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.238840.

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37

Finnis, Christopher J. A. "A DNA packaging system from the Streptomyces phage #phi#C31." Thesis, University of Nottingham, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.250469.

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38

Silva, de Siqueira Regina. "An evaluation of the phage amplification assay for detection of salmonella." Thesis, University of Nottingham, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.288778.

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39

Sandegren, Linus. "Group I Introns and Homing Endonucleases in T-even-like Bacteriophages." Doctoral thesis, Stockholm : Institutionen för molekylärbiologi och funktionsgenomik, Univ, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-211.

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40

Fàbrega, Ferrer Montserrat. "Structural characterization of the T7 bacteriophage portal protein." Doctoral thesis, Universitat de Barcelona, 2017. http://hdl.handle.net/10803/457690.

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The Escherichia coli infecting T7 bacteriophage shares a common dsDNA packaging mechanism with other bacteriophages of the Caudovirales order, Herpesviruses and Adenoviruses. The packaging machinery comprises the portal protein and the terminase complex. The portal protein is a channel located at a unique portal vertex that provides a conduit for DNA translocation, while the terminase complex recognizes a long concatemer of DNA, performs the nuclease catalytic activity and hydrolyses ATP. Available structural information about portal proteins describes them as oligomeric rings with an axial ch
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41

Wright, Alice Ann. "The Genomic Sequence and Annotation of Bacteriophage HK239." TopSCHOLAR®, 2010. http://digitalcommons.wku.edu/theses/208.

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Bacteriophages are viruses that infect bacteria and they are the most numerous biological entities on Earth. Temperate phage can adopt two different lifestyles. In the lytic lifestyle, a phage injects its genome into the host and a controlled developmental program ensues. The phage DNA is replicated, phage genes are expressed and new viral particles are assembled. Ultimately, the host cell lyses and the phage particles are released into the environment. In the lysogenic lifestyle, a phage integrates its genome into the host chromosome, creating a prophage. The cell containing the prophage is k
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42

Karunaratne, Desiree Nedra. "Structural investigation and bacteriophage degradation of bacterial polysaccharides." Thesis, University of British Columbia, 1985. http://hdl.handle.net/2429/25832.

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Seventy eight serologically distinct strains of Klebsiella bacteria are known to exist. The capsular polysaccharide surrounding the bacterial cell of these pathogenic Enterobacteria is of immunological significance. Structures of the capsular polysaccharides of nearly sixty seven strains of Klebsiella have been established, and each one found to be unique. The structures of the K antigens from Klebsiella, serotypes K67 and K80 are presented as a contribution to the continuing program of elucidation of the chemical structures of these antigens in an attempt to explain their immunological respon
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43

Lau, Mathew Thye Ngak Biotechnology &amp Biomolecular Sciences Faculty of Science UNSW. "Bacteriophage and phenotypic variation in Pseudomonas aeruginosa biofilms." Publisher:University of New South Wales. Biotechnology & Biomolecular Sciences, 2007. http://handle.unsw.edu.au/1959.4/40491.

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Pseudomonas aeruginosa is a ubiquitous environmental microorganism that opportunistically colonizes immune-compromised hosts. P. aeruginosa is capable of establishing complex. matrix-encased biofilms during colonization of both environmental and living host surfaces. Biofilms formed by P. aeruginosa are physiologically very different from free-living P. aeruginosa cells, and exhibit increased resistance to environmental stresses, including antibiotic treatment. While the development and establishment of P aeruginosa biofilms has been extensively studied in vitro, several new behaviours of P.
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44

Mosaico, Maria Luisa. "The utility of bacteriophage lambda in gene targeting." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0021/MQ55228.pdf.

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45

Sturino, Joseph Miland. "Bacteriophage Defense Systems and Strategies for Streptococcus thermophilus." NCSU, 2003. http://www.lib.ncsu.edu/theses/available/etd-12152003-034319/.

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The genomes of six Streptococcus thermophilus bacteriophages were compared to identify genes that could be targeted by engineered phage defense systems with potentially widespread efficacy. The genes associated with the S. thermophilus phage Sfi21-prototype genome replication module, including a putative primase and a putative helicase, were found to be among the best candidates due to their frequency of distribution in industrial phage isolates, striking sequence conservation between independent isolates, and intrinsic strategic importance in early phage development. Fourteen antisense RNA ca
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46

Gunneriusson, Elin. "Display of affinity proteins on bacteria and bacteriophage /." Stockholm : Department of Biotechnology, Royal Institute of Technology, 1999. http://www.lib.kth.se/abs99/gunn0521.pdf.

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47

Marks, James D. "Making human antibody fragments in bacteria and bacteriophage." Thesis, University of Cambridge, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.306239.

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48

Short, Nicholas J. "The DNA sequence of the filamentous bacteriophage Pf1." Thesis, University of Cambridge, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305822.

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49

Bryant, J. M. "Expression, mutagenesis and properties of bacteriophage K1E endosialidase." Thesis, University of Cambridge, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.597038.

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A full length endoE gene construct has been generated from the partial clones used to sequence the gene. Recombinant endoE has been expressed and purified as a fusion protein that is catalytically active and possesses the same kinetic characteristics as the bacteriophage enzyme. The mature, active endoE is 76 kDa in size and is probably produced by the post-translational cleavage of a 90 kDa primary translation product. Recombinant endoE has been purified to homogeneity, in milligram quantities, with the fusion protein partner removed for further studies of the enzyme. The role of the C-termin
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50

Reed, Patricia. "Function of bacteriophage Orf recombinases in genetic exchange." Thesis, Durham University, 2006. http://etheses.dur.ac.uk/4917/.

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Recombination events in bacteriophages frequently occur by illegitimate exchange at short tracts of sequence homology, enabling these viruses to acquire novel genes and serve as vehicles for horizontal gene transfer. The emergence of new pathogenic organisms due to the acquisition of virulence determinants from bacterial viruses has stimulated considerable interest in the mechanisms of phage recombination. Bacteriophage λ encodes its own recombination system, consisting of Exo, β and γ proteins. An additional λ recombinase, Orf, participates in the early stages of exchange, supplying a functio
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