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Artykuły w czasopismach na temat „Beta autoradiography”

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1

Takekawa, Shoichi, Yoshihiko Ueda, Yoshihiko Ueda, Yoshihiro Hiramatsu, Hirotsugu Munechika, and Fumio Shishido. "Imaging of Beta-Rays from Tissue Blocks with Thorotrast Deposition by Autoradiography using Fuji Computed Radiography." Jurnal Radiologi Indonesia 1, no. 2 (2015): 58–64. http://dx.doi.org/10.33748/jradidn.v1i2.7.

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Background: Autoradiography of tissue with radioactive substance such as Thorotrast by Fuji Computed Radiography (FCR) has been available. We obtained autoradiographs from Thorotrast-deposited tissue by FCR. However, the nature of radiation from tissue with Thorotrast was not certain, because alpha particles are shielded by the plastic front of the FCR cassette. Therefore, we undertook investigation to clearly explain the nature of radiation from Thorotrast in case of autoradiography.Materials and Methods: Tissue blocks of liver and spleen with Thorotrast deposition were imaged by autoradiogra

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2

Eakin, T. J., D. G. Baskin, J. F. Breininger, and W. L. Stahl. "Calibration of 14C-plastic standards for quantitative autoradiography with 33P." Journal of Histochemistry & Cytochemistry 42, no. 9 (1994): 1295–98. http://dx.doi.org/10.1177/42.9.8064137.

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Probes labeled with 33P have potential for widespread use in in situ hybridization because they are better able to detect relatively scarce mRNAs compared with probes labeled with 35S, but the relatively short half-life of 33P is a disadvantage when it is used as a radioactivity standard for quantitative autoradiography. To determine if plastic sections containing 14C can be used as standards for quantitative autoradiography with 33P, we co-exposed 33P-labeled liver paste sections and 14C-plastic standards to Hyperfilm beta max. The autoradiographic response of Hyperfilm beta max to these isot

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3

Birnkrant, D. J., P. B. Davis, and P. Ernsberger. "Visualization of high- and low-affinity beta-adrenergic receptors in rat lung: upregulation by chronic hypoxia." American Journal of Physiology-Lung Cellular and Molecular Physiology 265, no. 4 (1993): L389—L394. http://dx.doi.org/10.1152/ajplung.1993.265.4.l389.

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Chronic hypoxia induces a hyperadrenergic state which down-regulates beta-adrenergic receptors (beta-AR) in the heart. We visualized lung beta-AR binding sites after adaptation to chronic hypoxia by autoradiography of whole lung sections labeled with 50 pM 125I-labeled pindolol. A low concentration of agonist (32 nM isoproterenol) selectively masked beta-ARs with high affinity for agonists. Total specific beta-AR binding increased twofold with hypoxia. In both the control and hypoxic lung sections, 60–70% of the beta-ARs were in a high-affinity state, which could be reversed by guanine nucleot

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4

Lees, J. E., J. F. Pearson, G. W. Fraser, J. M. Hales, and P. G. Richards. "An MCP-based system for beta autoradiography." IEEE Transactions on Nuclear Science 46, no. 3 (1999): 636–38. http://dx.doi.org/10.1109/23.775591.

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5

Sanghera, B., D. M. Raymond, G. Hall, and R. J. Ott. "Digital beta autoradiography using silicon microstrip detectors." Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment 310, no. 1-2 (1991): 455–59. http://dx.doi.org/10.1016/0168-9002(91)91079-b.

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6

Rodriguez-Vieitez, Elena, Ruiqing Ni, Balázs Gulyás, et al. "Astrocytosis precedes amyloid plaque deposition in Alzheimer APPswe transgenic mouse brain: a correlative positron emission tomography and in vitro imaging study." Eur J Nucl Med Mol Imaging. 42, no. 7 (2015): 1119–32. https://doi.org/10.1007/s00259-015-3047-0.

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PURPOSE: Pathological studies suggest that neuroinflammation is exacerbated by increased beta-amyloid (Aβ) levels in the brain early in Alzheimer's disease (AD). The time course and relationships between astrocytosis and Aβ deposition were examined using multitracer in vivo positron emission tomography (PET) imaging in an AD transgenic mouse model, followed by postmortem autoradiography and immunohistochemistry analysis. METHODS: PET imaging with the amyloid plaque tracer (11)C-AZD2184 and the astroglial tracer (11)C-deuterium-L-deprenyl ((11)C-DED) was carried out in APPswe mice

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7

Lees, J. E., and G. W. Fraser. "Efficiency enhancements for MCP-based beta autoradiography imaging." Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment 477, no. 1-3 (2002): 239–43. http://dx.doi.org/10.1016/s0168-9002(01)01838-1.

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8

Lees, J. E., G. W. Fraser, and D. Dinsdale. "Direct beta autoradiography using microchannel plate (MCP) detectors." Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment 392, no. 1-3 (1997): 349–53. http://dx.doi.org/10.1016/s0168-9002(97)00219-2.

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9

Woodhead, C. J., and A. J. Nimmo. "Beta adrenoceptors in human nasal mucosa." Journal of Laryngology & Otology 105, no. 8 (1991): 632–34. http://dx.doi.org/10.1017/s0022215100116871.

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AbstractThe significance of the beta adrenergic system in the nasal mucosa isunclear. The authors have used the technique of autoradiography to localize and classify beta adrenoceptors in human nasal mucosa. The receptors have been found to beexclusively of thebeta-2 subtype and the highest density is found in the glandularducts. It is suggested that the beta-adrenergic system may have a physiologically important role in controlling the electrolyte composition of nasal secretions.

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10

Wide, M., H. Persson, S. Gunnarsson, L. Wide, and L. Seifi. "High frequency of in situ hybridization on thin plastic sections of human placenta with a cDNA probe for beta hCG." Journal of Histochemistry & Cytochemistry 37, no. 8 (1989): 1193–96. http://dx.doi.org/10.1177/37.8.2474022.

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We describe two different techniques with plastic embedding in in situ hybridization histochemistry (ISHH). Their applicability was demonstrated by use of human placenta of the tenth gestational week and a tritium-labeled cDNA probe for the beta-subunit of hCG. In the first method, ISHH was performed on whole pieces of tissue (en bloc ISHH) pretreated with a weak acid solution, embedded in methacrylate, and sectioned at 3 microns for autoradiography. In the second technique, en bloc ISHH was carried out on tissue pre-treated with the weak acid and thereafter with detergent to further facilitat

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11

Zhao, M., H. K. Hagler, and K. H. Muntz. "Regulation of alpha 1-, beta 1-, and beta 2-adrenergic receptors in rat heart by norepinephrine." American Journal of Physiology-Heart and Circulatory Physiology 271, no. 5 (1996): H1762—H1768. http://dx.doi.org/10.1152/ajpheart.1996.271.5.h1762.

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Previous studies suggest that the desensitization and downregulation of beta 1-adrenergic receptors (beta 1-AR) in the failing heart are the result of the elevated plasma catecholamine levels associated with this disease. To examine norepinephrine (NE)-induced regulation of cardiac adrenergic receptors, rats were infused with l-NE (200 micrograms.kg-1.h-1 for 7 days) or vehicle (0.001 N HCl) by implantation of osmotic minipumps. The technique of coverslip autoradiography was used to quantify alpha 1-adrenergic receptors (alpha 1-AR), beta 1-AR, and beta 2-AR in different tissue compartments of

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12

Verdurand, Mathieu, Elise Levigoureux, Sophie Lancelot, et al. "Amyloid-Beta Radiotracer [18F]BF-227 Does Not Bind to Cytoplasmic Glial Inclusions of Postmortem Multiple System Atrophy Brain Tissue." Contrast Media & Molecular Imaging 2018 (2018): 1–7. http://dx.doi.org/10.1155/2018/9165458.

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The accumulation of aggregated alpha-synuclein (α-syn) in multiple brain regions is a neuropathological hallmark of synucleinopathies. Multiple system atrophy (MSA) is a synucleinopathy characterized by the predominant cerebral accumulation of aggregated α-syn as cytoplasmic glial inclusions (CGI). A premortem diagnosis tool would improve early diagnosis and help monitoring disease progression and therapeutic efficacy. One Positron Emission Tomography (PET) study suggested [11C]BF-227 as a promising radiotracer for monitoring intracellular α-syn deposition in MSA patients. We sought to confirm

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13

Voutilainen, Mikko, Juuso Sammaljärvi, Eveliina Muuri, Jérôme Donnard, Samuel Duval, and Marja Siitari-Kauppi. "Digital autoradiography on C-14-labelled PMMA impregnated rock samples using the BeaverTM." MRS Advances 3, no. 21 (2018): 1161–66. http://dx.doi.org/10.1557/adv.2018.226.

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In Finland and Sweden the KBS-3 concept has been chosen for the disposal of spent nuclear fuel in crystalline rock. Recent experiments have shown that heterogeneity of rock may play a major role in the transport of radionuclides. Autoradiographic methods have been proven to be able to assist the characterization of heterogeneous structures. In this study we tested a novel filmless autoradiographic device called BeaverTM which applies a micro patterned gaseous detector in order to quantitatively map beta emissions from C-14 atoms. The studied samples were impregnated with C-14-labelled methylme

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14

Tolszczuk, M., and G. Pelletier. "Autoradiographic localization of beta-adrenoreceptors in rat uterus." Journal of Histochemistry & Cytochemistry 36, no. 12 (1988): 1475–79. http://dx.doi.org/10.1177/36.12.2848069.

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The inhibitory effects of catecholamines on uterine smooth muscle are known to be mediated through beta-adrenergic receptors. To investigate further the distribution of these receptors in the rat uterus, we utilized in vitro autoradiography using [125I]-cyanopindolol [CYP], a specific beta-receptor ligand that has equal activity for both beta 1- and beta 2-receptor subtypes. The specificity of the labeling and the characterization of receptor subtypes in different cell types were achieved by displacement of radioligand with increasing concentrations of zinterol, a beta-adrenergic agonist with

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15

Healy, D. P., P. A. Münzel, and P. A. Insel. "Localization of beta 1- and beta 2-adrenergic receptors in rat kidney by autoradiography." Circulation Research 57, no. 2 (1985): 278–84. http://dx.doi.org/10.1161/01.res.57.2.278.

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16

Pinto, J. E., A. J. Nazarali, T. Torda, and J. M. Saavedra. "Autoradiographic characterization of beta-adrenoceptors in rat heart valve leaflets." American Journal of Physiology-Heart and Circulatory Physiology 256, no. 3 (1989): H821—H827. http://dx.doi.org/10.1152/ajpheart.1989.256.3.h821.

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beta-Adrenoceptors were localized and characterized in valve leaflets of the rat heart. Sixteen micrometer-thick tissue sections containing the mitral and aortic valves were incubated with (-)3-[125I]iodocyanopindolol followed by autoradiography with computerized microdensitometry and comparison with 125I-labeled standards. beta-Adrenoceptors were present in all the valves studied. The selective beta 1-adrenoceptor antagonist CGP 20712 A (100 nM) displaced not more than 20% of the total binding sites, suggesting that most of the beta-adrenoceptors in the valve leaflets are of the beta 2-subtyp

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17

Stoppacciaro, A., P. Bossu, P. Ghiara, et al. "Binding of IL-1 beta to IL-1R type II at single cell level." Journal of Immunology 147, no. 5 (1991): 1561–66. http://dx.doi.org/10.4049/jimmunol.147.5.1561.

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Abstract To gain information on the possible biologic role of IL-1R type II (IL-1RII), expression of the 68-kDa IL-1 binding protein on human lymphoblastoid B cells was investigated at single cell level. Binding of iodinated IL-1 beta was evaluated by autoradiography on cytosmears of IL-1RII positive B cell lines RAJI, the RAJI clone 1H7, and STS 25. Results obtained suggest an heterogeneity of IL-1RII expression within the B cell population, with only 5 to 16% of the cells able to bind IL-1 beta. Up-regulation of IL-1RII expression by dexamethasone, evident in conventional binding assays, was

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18

Haddad, A., B. Kopriwa, and G. Pelletier. "Localization of glycoproteins in insulin secretory granules by ultrastructural autoradiography." Journal of Histochemistry & Cytochemistry 35, no. 10 (1987): 1059–62. http://dx.doi.org/10.1177/35.10.3305700.

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To determine whether or not the secretory granules of insulin-secreting cells contained glycoproteins, isolated rat pancreatic islets were incubated for 2 and 4 hr in a medium containing L-[3H]-fucose. Quantitative analysis of high-resolution electron microscopic autoradiographs of the insulin-secreting beta cells demonstrated that glycoproteins with fucose residues are contained within the insulin secretory granule.

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19

Ohira, Y., W. Yasui, F. Kariya, et al. "Spaceflight effects on beta-adrenoceptor and metabolic properties in rat plantaris." Journal of Applied Physiology 81, no. 1 (1996): 152–55. http://dx.doi.org/10.1152/jappl.1996.81.1.152.

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Effects of 14 days of spaceflight on beta-adrenoceptor (beta-AR), mitochondrial enzyme activities, and fiber type composition were studied in plantaris muscles of male adult Sprague-Dawley rats. The beta-AR was analyzed in cross sections by quantitative autoradiography. The maximum binding capacity (Bmax) of beta-AR was significantly lowered (approximately 29%) after flight, but the recovery was not completed within 9 days in 1-G environment. Because the dissociation constant remained unchanged, it is suggested that the changes in the Bmax were caused by the alteration of receptor number. The

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20

Saito, K., M. Kurihara, R. Cruciani, W. Z. Potter, and J. M. Saavedra. "Characterization of beta 1- and beta 2-adrenoceptor subtypes in the rat atrioventricular node by quantitative autoradiography." Circulation Research 62, no. 1 (1988): 173–77. http://dx.doi.org/10.1161/01.res.62.1.173.

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21

IIDA, Takao, Yukimasa IKEBE, and Osamu MATSUOKA. "Autoradiography of .ALPHA.-and .BETA.-emitters using a charged-particle imaging system." RADIOISOTOPES 36, no. 7 (1987): 317–24. http://dx.doi.org/10.3769/radioisotopes.36.7_317.

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22

Summers, R. J., J. A. Stephenson, and M. J. Kuhar. "Localization of beta adrenoceptor subtypes in rat kidney by light microscopic autoradiography." Journal of Pharmacology and Experimental Therapeutics 232, no. 2 (1985): 561–69. https://doi.org/10.1016/s0022-3565(25)20601-2.

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23

Leskinen, Anumaija, Pascal Fichet, Marja Siitari-Kauppi, and Florence Goutelard. "Digital autoradiography (DA) in quantification of trace level beta emitters on concrete." Journal of Radioanalytical and Nuclear Chemistry 298, no. 1 (2013): 153–61. http://dx.doi.org/10.1007/s10967-013-2535-6.

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Wells, K., R. J. Ott, and J. H. MacDonald. "6. A novel CCD-based digital autoradiography system for quantitative beta imaging." Nuclear Medicine Communications 22, no. 4 (2001): 436. http://dx.doi.org/10.1097/00006231-200104000-00018.

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Greipp, PR, JA Katzmann, WM O'Fallon, and RA Kyle. "Value of beta 2-microglobulin level and plasma cell labeling indices as prognostic factors in patients with newly diagnosed myeloma." Blood 72, no. 1 (1988): 219–23. http://dx.doi.org/10.1182/blood.v72.1.219.219.

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Abstract Beta 2-microglobulin (beta 2M) has been proposed as a prognostic factor in multiple myeloma (MM), but beta 2M levels are reported to correlate with other prognostic indicators such as stage and creatinine level. This study addressed the independent prognostic values of these and other variables, including plasma cell labeling indices (LI), in patients with newly diagnosed MM. beta 2M levels were measured with an enzyme-linked immunosorbent assay. LI were determined with a [3H]thymidine autoradiography method. By multivariate analysis and Kaplan-Meier survival analysis, the uncorrected

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26

Greipp, PR, JA Katzmann, WM O'Fallon, and RA Kyle. "Value of beta 2-microglobulin level and plasma cell labeling indices as prognostic factors in patients with newly diagnosed myeloma." Blood 72, no. 1 (1988): 219–23. http://dx.doi.org/10.1182/blood.v72.1.219.bloodjournal721219.

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Beta 2-microglobulin (beta 2M) has been proposed as a prognostic factor in multiple myeloma (MM), but beta 2M levels are reported to correlate with other prognostic indicators such as stage and creatinine level. This study addressed the independent prognostic values of these and other variables, including plasma cell labeling indices (LI), in patients with newly diagnosed MM. beta 2M levels were measured with an enzyme-linked immunosorbent assay. LI were determined with a [3H]thymidine autoradiography method. By multivariate analysis and Kaplan-Meier survival analysis, the uncorrected beta 2M

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27

Teng, Yu, Hong Yang, and Yulin Tian. "The Development and Application of Tritium-Labeled Compounds in Biomedical Research." Molecules 29, no. 17 (2024): 4109. http://dx.doi.org/10.3390/molecules29174109.

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With low background radiation, tritiate compounds exclusively emit intense beta particles without structural changes. This makes them a useful tool in the drug discovery arsenal. Thanks to the recent rapid progress in tritium chemistry, the preparation and analysis of tritium-labeled compounds are now much easier, simpler, and cheaper. Pharmacokinetics, autoradiography, and protein binding studies have been much more efficient with the employment of tritium-labeled compounds. This review provides a comprehensive overview of tritium-labeled compounds regarding their properties, synthesis strate

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28

Ghinea, N., A. Fixman, D. Alexandru, et al. "Identification of albumin-binding proteins in capillary endothelial cells." Journal of Cell Biology 107, no. 1 (1988): 231–39. http://dx.doi.org/10.1083/jcb.107.1.231.

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Isolated fat tissue microvessels and lung, whose capillary endothelia express in situ specific binding sites for albumin, were homogenized and subjected to SDS-gel electrophoresis and electroblotting. The nitrocellulose strips were incubated with either albumin-gold (Alb-Au) and directly visualized, or with [125I]albumin (monomeric or polymeric) and autoradiographed. The extracts of both microvascular endothelium and the lung express albumin-binding proteins (ABPs) represented by two pairs of polypeptides with major components of molecular mass 31 and 18 kD. The ABP peptides have pIs 8.05 to 8

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29

Park, Hae-Young, Kyeong-Han Park, Yong-Sik Kim, Sang-Goo Shin, Myung-Hee Chung, and Chan-Woong Park. "Quantitative Autoradiography of Beta-1 and Beta-2 Adrenergic Receptor and Cellular Localization of mRNA for Beta-1 Adrenergic Receptor in Rat Brain." Molecules and Cells 6, no. 4 (1996): 456–62. http://dx.doi.org/10.1016/s1016-8478(23)07275-8.

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30

Kusumoto, F. M., K. G. Lurie, J. Dutton, H. Capili, and J. B. Schwartz. "Effects of aging on AV nodal and ventricular beta-adrenergic receptors in the Fischer 344 rat." American Journal of Physiology-Heart and Circulatory Physiology 266, no. 4 (1994): H1408—H1415. http://dx.doi.org/10.1152/ajpheart.1994.266.4.h1408.

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The effects of aging on atrioventricular (AV) nodal and right and left ventricular beta-adrenergic receptor (beta-AR) characteristics were studied in Fischer 344 rat hearts using quantitative autoradiography. Twenty-micrometer-thick cardiac sections containing the compact AV node from 16 mature (4-6 mo old) rats, 6 middle-aged (12 mo old) rats, and 16 old (24 mo old) rats were incubated with [125I]iodocyanopindolol, [125I]iodocyanopindolol plus atenolol, or ICI 118551. Saturation experiments revealed a significant age-related decrease in AV nodal beta-AR density (mature: 190 +/- 46, middle-age

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31

Miller, Brian W. "Radiation Imagers for Quantitative, Single-particle Digital Autoradiography of Alpha- and Beta-particle Emitters." Seminars in Nuclear Medicine 48, no. 4 (2018): 367–76. http://dx.doi.org/10.1053/j.semnuclmed.2018.02.008.

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Yorke, E. D., L. E. Williams, A. J. Demidecki, D. B. Heidorn, P. L. Roberson, and B. W. Wessels. "Multicellular dosimetry for beta-emitting radionuclides: Autoradiography, thermoluminescent dosimetry and three-dimensional dose calculations." Medical Physics 20, no. 2 (1993): 543–50. http://dx.doi.org/10.1118/1.597050.

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Belyanin, Dmitriy, Sergey Zhmodik, Evgeniya Airiyants, and Ivan Kirichenko. "Local distribution of cobalt and selective enrichment of platinum in oceanic ferromanganese nodules." E3S Web of Conferences 98 (2019): 05002. http://dx.doi.org/10.1051/e3sconf/20199805002.

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The distribution of cobalt in the structure of ferromanganese nodules (FMN) was studied using beta-autoradiography. In some cases, the content of element increased in the outer part of the nodules, or in thin layers within the nodules. Layered type of cobalt distribution in FMN indicates changing conditions in deposition during their formation, in particular also the living conditions of microorganisms known to inhabit oceanic ferromanganese nodules. Experimental studies suggested that colonies of micromycetes living at the surface of the nodules can have an important impact on the selective a

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Fava, R. A., N. J. Olsen, A. E. Postlethwaite, et al. "Transforming growth factor beta 1 (TGF-beta 1) induced neutrophil recruitment to synovial tissues: implications for TGF-beta-driven synovial inflammation and hyperplasia." Journal of Experimental Medicine 173, no. 5 (1991): 1121–32. http://dx.doi.org/10.1084/jem.173.5.1121.

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We have studied the consequences of introducing human recombinant transforming growth factor beta 1 (hrTGF-beta 1) into synovial tissue of the rat, to begin to better understand the significance of the fact that biologically active TGF-beta is found in human arthritic synovial effusions. Within 4-6 h after the intra-articular injection of 1 microgram of hrTGF-beta 1 into rat knee joints, extensive recruitment of polymorphonuclear leukocytes (PMNs) was observed. Cytochemistry and high resolution histological techniques were used to quantitate the influx of PMNs, which peaked 6 h post-injection.

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Helqvist, Steffen, Birgit Sehested Hansen, Jesper Johannesen, Henrik Ullits Andersen, Jens Høiriis Nielsen, and Jørn Nerup. "Interleukin 1 induces new protein formation in isolated rat islets of Langerhans." Acta Endocrinologica 121, no. 1 (1989): 136–40. http://dx.doi.org/10.1530/acta.0.1210136.

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Abstract. It is hypothesized that interleukin 1 induces toxic free radical formation in pancreatic beta-cells leading to beta-cell degeneration and destruction. Therefore, isolated rat pancreatic islets were examined for interleukin 1 and heat shock induced proteins. After exposure to human interleukin 1β (0.015 to 10 μg/1) or heat (40°C) for up to 24 h, islets were labelled with 35S-methionine and solubilized. Islets proteins were analysed by SDS-polyacrylamide gel electrophoresis. By autoradiography it was shown that both interleukin 1 and heat exposure induced the formation of a 70 kD prote

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36

Legrand, C., V. Vivat, C. Rigolot, and J. P. Maltier. "Selective distribution of alpha-1 and beta adrenoceptors in pregnant rat uterus visualized by autoradiography." Journal of Pharmacology and Experimental Therapeutics 256, no. 2 (1990): 767–72. https://doi.org/10.1016/s0022-3565(25)23110-x.

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Crothers, J. M., D. C. Chow, M. L. Scalley, and J. G. Forte. "In vivo trafficking of nascent H(+)-K(+)-ATPase in rabbit parietal cells." American Journal of Physiology-Gastrointestinal and Liver Physiology 269, no. 6 (1995): G883—G891. http://dx.doi.org/10.1152/ajpgi.1995.269.6.g883.

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Protein metabolic labeling in vivo was used to determine a time course for trafficking of nascent H(+)-K(+)-adenosinetriphosphatase (H(+)-K(+)-ATPase) from endoplasmic reticulum (ER) to mature tubulovesicles in parietal cells. Stomachs of cimetidine-treated rabbits were taken 15-90 min after injection of [35S]methionine/cysteine, and mucosal microsomes were fractionated on sucrose gradients for analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blot, and autoradiography. After 15 min, labeled alpha-subunit peaked at approximately 1.14 g/ml, matching the distribution

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Clark, S. A., W. E. Stumpf, M. Sar, and H. F. DeLuca. "1,25-Dihydroxyvitamin D3 target cells in immature pancreatic islets." American Journal of Physiology-Endocrinology and Metabolism 253, no. 1 (1987): E99—E105. http://dx.doi.org/10.1152/ajpendo.1987.253.1.e99.

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Target cells of 1,25-dihydroxyvitamin D3 were identified by autoradiography in islets from rats of different ages. Nuclei of pancreatic islet cells selectively concentrated 1,25-[3H]dihydroxyvitamin D3 but not 25-[3H]hydroxyvitamin D3 or 24,25-[3H]dihydroxyvitamin D3. Developmental studies of pancreatic islets indicated that target cells, as revealed by significant nuclear concentration of 1,25-[3H]dihydroxyvitamin D3, are present in islet cells of fetal rats. The percentage of islet cells that concentrated 1,25-[3H]dihydroxyvitamin D3 increased from 10 to 15% in the fetus to 60% at 1 day of a

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Hall, S. W., J. LaMarre, L. B. Marshall, M. A. Hayes та S. L. Gonias. "Binding of transforming growth factor-β1 to methylamine-modified α2-macroglobulin and to binary and ternary α2-macroglobulin-proteinase complexes". Biochemical Journal 281, № 2 (1992): 569–75. http://dx.doi.org/10.1042/bj2810569.

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The binding of 125I-labelled transforming growth factor-beta 1 (TGF-beta 1) to human alpha 2-macroglobulin (alpha 2M) was studied by native PAGE and autoradiography. TGF-beta 1 bound preferentially to alpha 2M-methylamine and minimally, if at all, to native alpha 2M. Preparations of alpha 2M-proteinase complex were generated by incubating a standard concentration of alpha 2M (0.4 microM) with different concentrations of trypsin, chymotrypsin or neutrophil elastase (0.04-2.0 microM). The 125I-TGF-beta 1-binding activity depended on the initial ratio of active proteinase to alpha 2M, or r value,

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Borth, W., A. Urbanski, R. Prohaska, M. Susanj, and TA Luger. "Binding of recombinant interleukin-1 beta to the third complement component and alpha 2-macroglobulin after activation of serum by immune complexes." Blood 75, no. 12 (1990): 2388–95. http://dx.doi.org/10.1182/blood.v75.12.2388.2388.

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Abstract Activation of human normal serum with tetanus/antitetanus immune complexes (TAT-IC) resulted in increased binding of 125I-labeled interleukin-1 beta (IL-1 beta) to serum factors, as opposed to untreated serum. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by autoradiography showed labeling of two large molecular mass factors of an apparent molecular weight (Mr) of 200,000 and 400,000, respectively. These complexes could be dissociated by reduction. No complexes were formed when reducing compounds were added to serum-TAT-IC-125I-IL-1 beta mixtures. Compl

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Borth, W., A. Urbanski, R. Prohaska, M. Susanj, and TA Luger. "Binding of recombinant interleukin-1 beta to the third complement component and alpha 2-macroglobulin after activation of serum by immune complexes." Blood 75, no. 12 (1990): 2388–95. http://dx.doi.org/10.1182/blood.v75.12.2388.bloodjournal75122388.

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Activation of human normal serum with tetanus/antitetanus immune complexes (TAT-IC) resulted in increased binding of 125I-labeled interleukin-1 beta (IL-1 beta) to serum factors, as opposed to untreated serum. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by autoradiography showed labeling of two large molecular mass factors of an apparent molecular weight (Mr) of 200,000 and 400,000, respectively. These complexes could be dissociated by reduction. No complexes were formed when reducing compounds were added to serum-TAT-IC-125I-IL-1 beta mixtures. Complex format

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Hellberg, Sanna, Johanna Silvola, Heidi Liljenbäck, et al. "Amyloid-Targeting PET Tracer [18F]Flutemetamol Accumulates in Atherosclerotic Plaques." Molecules 24, no. 6 (2019): 1072. http://dx.doi.org/10.3390/molecules24061072.

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Atherosclerosis is characterized by the accumulation of oxidized lipids in the artery wall, which triggers an inflammatory response. Oxidized low-density lipoprotein (ox-LDL) presents amyloid-like structural properties, and different amyloid species have recently been recognized in atherosclerotic plaques. Therefore, we studied the uptake of the amyloid imaging agent [18F]Flutemetamol in atherosclerotic plaques. The binding of [18F]Flutemetamol to human carotid artery plaque was studied in vitro. In vivo uptake of the tracer was studied in hypercholesterolemic IGF-II/LDLR−/−ApoB100/100 mice an

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Bateman, J. E., J. F. Connolly, and R. Stephenson. "High speed quantitative digital beta autoradiography using a multistep avalanche detector and an Apple II microcomputer." Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment 241, no. 1 (1985): 275–89. http://dx.doi.org/10.1016/0168-9002(85)90546-7.

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Barthe, N., K. Chatti, P. Coulon, S. Maı̂trejean, and B. Basse-Cathalinat. "Recent technologic developments on high-resolution beta imaging systems for quantitative autoradiography and double labeling applications." Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment 527, no. 1-2 (2004): 41–45. http://dx.doi.org/10.1016/j.nima.2004.03.014.

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Saffitz, J. E., and S. B. Liggett. "Subcellular distribution of beta 2-adrenergic receptors delineated with quantitative ultrastructural autoradiography of radioligand binding sites." Circulation Research 70, no. 6 (1992): 1320–25. http://dx.doi.org/10.1161/01.res.70.6.1320.

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Barnes, J. L., K. A. Woodruff, S. P. Levine, and H. E. Abboud. "Inhibition of mesangial cell proliferation by platelet factor 4." Journal of the American Society of Nephrology 7, no. 7 (1996): 991–98. http://dx.doi.org/10.1681/asn.v77991.

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Platelet factor 4(PF4), an abundant platelet secretory product, is a strong candidate for modulating glomerular pathology. Because PF4 might be released from platelets and influence intrinsic cell growth during glomerular injury, the effect of PF4 on fetal calf serum- and platelet-derived growth factor (PDGF)-induced mesangial cell mitogenesis was examined. Mitogenesis was measured as the amount of 3H-thymidine incorporated into acid-precipitable material as well as by autoradiography. The effect of PF4 on mesangial cell expression of mRNA for PDGF A chain and transforming growth factor-beta (

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Alibardi, Lorenzo. "The Periodic Replacement of Adhesive Setae in Pad Lamellae of Climbing Lizards Is Driven by Patterns of Corneous Layer Growth." Journal of Developmental Biology 11, no. 1 (2022): 3. http://dx.doi.org/10.3390/jdb11010003.

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The adhesive digital pads in some gecko and anoline lizards are continuously utilized for movements on vertical surfaces that may determine wear and a decrease of adhesion efficiency. The pads are formed by lamellae bearing adhesive setae that are worn out following frequent usage and are replaced by new inner setae that maintain an efficient adhesion. Whether the extensive usage of adhesive setae determines a higher shedding frequency in the digital pads with respect to other body regions remains unknown. Setae replacement has been analyzed in embryos and adult lizards using autoradiography a

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Hawkins, R. A., A. M. Mans, and D. W. Davis. "Regional ketone body utilization by rat brain in starvation and diabetes." American Journal of Physiology-Endocrinology and Metabolism 250, no. 2 (1986): E169—E178. http://dx.doi.org/10.1152/ajpendo.1986.250.2.e169.

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The rate of ketone body (beta-hydroxybutyrate and acetoacetate) metabolism was measured in individual cerebral structures of fed, starved, and diabetic rats. This was done by infusing beta-[3-14C]hydroxybutyrate intravenously and measuring the incorporation of 14C into brain by quantitative autoradiography. The capacity of the brain to use ketone bodies, expressed as plasma clearance, increased in starvation and diabetes by approximately 50-60%. Plasma clearance was near maximal after 2 days starvation and was not significantly increased after 4 days starvation, 6 days of diabetes or 28 days o

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Zhang, R. S., P. H. Guth, O. U. Scremin, and G. Chaudhuri. "H2 gas clearance technique for separating rat uterine blood flow into endometrial and myometrial components." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 268, no. 2 (1995): R569—R575. http://dx.doi.org/10.1152/ajpregu.1995.268.2.r569.

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The H2 gas clearance technique was employed to measure uterine blood flow (UBF) in ovariectomized rats. A needle-type platinum electrode (125 microns diam) was inserted into the rat uterine wall to measure the tissue blood flow surrounding the electrode. The electrode can be placed in individual layers of the uterus to measure the endometrial blood flow (EBF) or the myometrial blood flow (MBF). By use of this technique, baseline EBF and MBF were 37.8 +/- 3.53 (n = 21) and 47.2 +/- 4.56 (n = 5) ml.min-1.100 g-1, respectively, with an EBF/MBF ratio of 0.8. Intravenous bolus injection of 17 beta-

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SATO, Tachio, Takehiko FUJIWARA, Yoshinao ABE, et al. "Double tracer whole body autoradiography using a short-lived positron emitter and a long-lived .BETA. emitter." RADIOISOTOPES 38, no. 1 (1989): 7–12. http://dx.doi.org/10.3769/radioisotopes.38.7.

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