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Cuevas-Sierra, Amanda, Fermín I. Milagro, Paula Aranaz, Jose Alfredo Martínez, and José I. Riezu-Boj. "Gut Microbiota Differences According to Ultra-Processed Food Consumption in a Spanish Population." Nutrients 13, no. 8 (2021): 2710. http://dx.doi.org/10.3390/nu13082710.

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Ultra-processed foods (UPFs) consumption could affect gut microbiota diversity and profile. We aimed to evaluate the effects of UPFs on microbiota, considering the role of sex. The consumption of UPFs (using NOVA criteria) was assessed with a validated 137-item food-frequency questionnaire. Participants (n = 359) were classified into less than three servings per day (n = 96) of UPFs and more than five (n = 90). Women and men were subclassified following the same criteria. 16S rRNA sequencing was performed from DNA fecal samples, and differences in microbiota were analyzed using EdgeR. The rela
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Akagawa, Shohei, Yuko Akagawa, Sohsaku Yamanouchi, et al. "Association of Neonatal Jaundice with Gut Dysbiosis Characterized by Decreased Bifidobacteriales." Metabolites 11, no. 12 (2021): 887. http://dx.doi.org/10.3390/metabo11120887.

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Neonatal jaundice, caused by excess serum bilirubin levels, is a common condition in neonates. Imbalance in the gut microbiota is believed to play a role in the development of neonatal jaundice. Thus, we aimed to reveal the gut microbiota characteristics in neonates with jaundice. 16S rRNA gene sequencing was performed on stool samples collected on day 4 from 26 neonates with jaundice (serum total bilirubin > 15.0 mg/dL) and 17 neonates without jaundice (total serum bilirubin < 10.0 mg/dL). All neonates were born full term, with normal weight, by vaginal delivery, and were breastfed. Neo
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Wang, Qi, Huajie Dai, Tianzhichao Hou, et al. "Dissecting Causal Relationships Between Gut Microbiota, Blood Metabolites, and Stroke: A Mendelian Randomization Study." Journal of Stroke 25, no. 3 (2023): 350–60. http://dx.doi.org/10.5853/jos.2023.00381.

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Background and Purpose We investigated the causal relationships between the gut microbiota (GM), stroke, and potential metabolite mediators using Mendelian randomization (MR).Methods We leveraged the summary statistics of GM (n=18,340 in the MiBioGen consortium), blood metabolites (n=115,078 in the UK Biobank), and stroke (cases n=60,176 and controls n=1,310,725 in the Global Biobank Meta-Analysis Initiative) from the largest genome-wide association studies to date. We performed bidirectional MR analyses to explore the causal relationships between the GM and stroke, and two mediation analyses,
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Yudina, Julia V., Alfiia I. Aminova, Andrey P. Prodeus, and Anatoly A. Korsunskiy. "Changes in Intestinal Microbiota Composition in 1–5 Years Old Children with Atopic Dermatitis: Cross Sectional Study." Pediatric pharmacology 18, no. 5 (2021): 377–84. http://dx.doi.org/10.15690/pf.v18i5.2294.

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Background. Atopic dermatitis (AD) arouses high research interest these days due to its significant morbidity rate. The most crucial risk factor for its development is the intestinal microbiota composition. The correlation of this factor with the development of AD in children requires further study.Objective. The aim of the study is to perform comparative analysis of the intestinal microbiota in 1–5 years old children with AD and conditionally healthy children via 16S-sequencing of ribosomal RNA (rRNA) of bacterial genes.Methods. We have conducted cross sectional study. 60 children with diagno
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Li, Renjie, Yi Xu, and Tao Cheng. "Gut microbiota affects aneurysms through biomechanical mechanisms: A mendelian randomization study." Molecular & Cellular Biomechanics 21, no. 4 (2024): 303. https://doi.org/10.62617/mcb303.

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Purpose: To assess any potential associations between gut microbiota (GM) and aortic aneurysm and cerebral aneurysm in biomechanics (AA and CA). Methods: We performed a two-sample Mendelian randomization (MR) to assess the causal association between GM and AA, CA. The inverse-variance weighted (IVW) model was used as the main analytical method and was followed by sensitivity analysis, including heterogeneity test, horizontal pleiotropy test, and leave-one-out analysis, to appraise the robustness of the MR results. Results: Our IVW results showed that RuminococcaceaeUCG005 [OR = 1.43, 95% CI (1
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Uchida, Kenji, Kenichi Iida, Ikumi Fujioka, Satoshi Hachimura, and Osamu Kaminuma. "Suppressive Effect of Lactococcus lactis subsp. cremoris YRC3780 on a Murine Model of Japanese Cedar Pollinosis." Pathogens 11, no. 11 (2022): 1347. http://dx.doi.org/10.3390/pathogens11111347.

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Accumulating evidence suggests that Lactococcus lactis subsp. cremoris YRC3780 isolated from kefir has the potential to alleviate allergic responses. Herein, we investigated the effect of YRC3780 on a murine model of Japanese cedar pollinosis (JCP). BALB/c mice immunized with cedar pollen extract (CPE) exhibited an increase in serum immunoglobulin E and developed nasal inflammatory responses including sneezing, nasal hyperresponsiveness, and nasal eosinophil accumulation upon intranasal allergen challenge. These responses were suppressed by the oral administration of YRC3780, although the effe
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7

Geng, Ningning, Ying Li, Yan Zhang, et al. "Effects of Modified Dietary Fiber from Fresh Corn Bracts on Obesity and Intestinal Microbiota in High-Fat-Diet Mice." Molecules 28, no. 13 (2023): 4949. http://dx.doi.org/10.3390/molecules28134949.

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The effects of insoluble dietary fiber from fresh corn bracts modified by dynamic high-pressure micro-fluidization (DHPM) on the pathological characteristics of obesity, intestinal microflora distribution and production of short-chain fatty acids in high-fat-diet C57BL/6 mice were evaluated. The results show that the DHPM-modified dietary fiber from fresh corn bracts significantly reduces weight gain, insulin resistance and oxidative damage caused by a high-fat diet, and promotes the production of SCFAs, especially acetic acid, propionic acid and butyric acid. These modified dietary fibers als
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Zhuang, Huiren, Nan Jing, Luoyang Wang, Guoqiang Jiang, and Zheng Liu. "Jujube Powder Enhances Cyclophosphamide Efficiency against Murine Colon Cancer by Enriching CD8+ T Cells While Inhibiting Eosinophilia." Nutrients 13, no. 8 (2021): 2700. http://dx.doi.org/10.3390/nu13082700.

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Cyclophosphamide (CTX) is widely applied in cancer treatment. However, the outcome is often compromised by lymphopenia, myelosuppression, and gut dysbiosis. Here, we used jujube powder to enhance CTX efficiency through nurturing gut microbiota in order to facilitate favorable metabolisms. It was observed that the oral administration of jujube powder enriched CD8+ T cells in mouse MC38 colon tumor microenvironment and increased the diversity of gut microbiota and the abundance of Bifidobacteriales, which is helpful to the production of butyrate in the cecum content. The application of jujube po
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Kusumaningrum, Tina, Wisnu Tafroji, Septiani Madonna Gultom, Nina Dwi Putri, Cut Nurul Hafifah, and Dodi Safari. "Gut Microbiota Profile of Infants with Breastfeeding and Mixed Feeding Patterns." HAYATI Journal of Biosciences 31, no. 3 (2024): 530–38. http://dx.doi.org/10.4308/hjb.31.3.530-538.

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We explore the gut microbiota profiles of 103 stool samples collected from infants at the age of 4 and 6 months in Jakarta, Indonesia. We performed 16S rRNA gene sequencing with Illumina MiSeq to identify the diversity, structure, and composition of the gut microbiota from those stool samples. Among 103 stool samples, 55 and 48 samples were collected from infants with breastfeeding and mixed feeding patterns, respectively. We found that the most abundant bacteria were Bifidobacteriales from the phylum of Actinobacteria (43.05%), Lactobacillales from the phylum of Firmicutes (28.39%), and Enter
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Yang, Lina, Yafan Zhao, Jinghang Huang, et al. "Insoluble dietary fiber from soy hulls regulates the gut microbiota in vitro and increases the abundance of bifidobacteriales and lactobacillales." Journal of Food Science and Technology 57, no. 1 (2019): 152–62. http://dx.doi.org/10.1007/s13197-019-04041-9.

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Monteleone, Alessio Maria, Jacopo Troisi, Gloria Serena, et al. "The Gut Microbiome and Metabolomics Profiles of Restricting and Binge-Purging Type Anorexia Nervosa." Nutrients 13, no. 2 (2021): 507. http://dx.doi.org/10.3390/nu13020507.

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Alterations in the gut microbiome and fecal metabolites have been detected in anorexia nervosa (AN), but differences in those profiles between restricting AN (ANR) and binge-purging AN (ANBP) type have not been explored. We made a secondary analysis of our previous data concerning microbiome and metabolomics profiles of 17 ANR women, six ANBP women and 20 healthy controls (HC). Twelve fecal metabolites differentiating ANR patients, ANBP patients and HC were identified. Both patient groups showed decreased intra-individual bacterial richness with respect to healthy controls (HC). Compared to AN
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Nikonov, I. N., L. A. Il'ina, I. I. Kochish та ін. "Изменение микробиоты кишечника кур в онтогенезе". Ukrainian Journal of Ecology 7, № 4 (2017): 492–99. http://dx.doi.org/10.15421/2017_150.

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<p>This paper presents the results of a molecular genetic analysis of the changes in the composition of the microbiota of the blind processes of the intestine of the hens of the industrial loam "Lohmann Brown" during ontogeny. According to the results of the analysis of taxonomic affiliation it is established that over 70% of the phylotypes belong to the three phylums - Firmicutes, Bacteroidetes and Proteobacteria, less represented were Actinobacteria, Tenericutes and Fusobacteria, and a significant number of unidentified bacteria was detected. During ontogenesis, birds exhibited marked
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Loughrin, John H., and Getahun E. Agga. "The Effect of Mono- and Di-Saccharides on the Microbiome of Dairy Cow Manure and Its Odor." Microorganisms 13, no. 1 (2024): 52. https://doi.org/10.3390/microorganisms13010052.

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In a previous experiment, we showed that the odor of Bos taurus manure slurries could be improved by anaerobic incubation with the sugars glucose, lactose, and sucrose. This improvement was due to reductions in the concentrations of malodorants, including dimethyl disulfide, p-cresol, p-ethylphenol, indole, and skatole, and a shift to the production of fruity esters, including ethyl butyrate and propyl propanoate. Due to large concentrations of lactic acid produced by the sugar-amended manure slurries, we inferred that lactic acid bacteria were involved in improving the manure slurry odor. Her
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Cui, Yiyan, Zhichang Liu, Dun Deng, et al. "Influence of Fermented Mulberry Leaves as an Alternative Animal Feed Source on Product Performance and Gut Microbiome in Pigs." Fermentation 10, no. 4 (2024): 215. http://dx.doi.org/10.3390/fermentation10040215.

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Mulberry leaves are rich in nutrients but contain anti-nutrient factors that hinder their digestion and absorption. Feeding animals with mulberry leaves directly could harm their health. The microbial fermentation of mulberry leaves could reduce their anti-nutritional factors’ content and improve their nutritional value. Sequencing and analyzing mulberry leaves before and after fermentation showed that fermentation increased the relative abundance of Pediococcus, Bradyrhizobium, Hydrotalea, and Rhodanobacteria, and decreased that of Enterobacter. Fermentation improved the quality of mulberry l
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Herrera-Mejía, Julián, Abraham Wall-Medrano, Arnulfo Ramos-Jiménez, Aarón F. González-Córdova, Florinda Jiménez-Vega, and Rocio Campos-Vega. "Colonic Dysbiosis Is Associated with Gastrointestinal Disorders More than Mother–Child Epidemiological Traits in Mexican Children with Autism Spectrum Disorders." Gastroenterology Insights 16, no. 2 (2025): 15. https://doi.org/10.3390/gastroent16020015.

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Introduction: Functional gastrointestinal disorders (FGIDs) are common comorbidities that affect the life quality of children with autism. Objective: This study investigated the link between clinical history and specific colonic fecal microbiota (CFM) markers with the pathophysiology of FGIDs in young children with autism patients. Methods: Thirty-nine young patients (2 and 18 years) were included in the study of FGIDs (+) cases (n = 18) and FGIDs (-) (n = 21) controls. Gastrointestinal disorders were diagnosed by standardized clinical tools (ROMA-IV and six-item gastrointestinal severity inde
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Rolinec, Michal, Juraj Medo, Michal Gábor, et al. "The Effect of Coconut Oil Addition to Feed of Pigs on Rectal Microbial Diversity and Bacterial Abundance." Animals 10, no. 10 (2020): 1764. http://dx.doi.org/10.3390/ani10101764.

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Coconut oil has a high content of lauric acid, which has selective antibacterial activity. This study aimed to explore the effect of coconut oil ingestion on the gastrointestinal microbiomes of pigs. A 14-day-long feeding experiment included 19 pigs in two groups (9 on a normal diet and 10 on a diet supplemented with coconut oil). At the start and end of the experiment, a rectal swab sample was taken from each pig in both groups, and total bacterial DNA was extracted. We used 16S rRNA high-throughput amplicon sequencing to evaluate the microbiome changes during the feeding experiment. A total
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Strassert, Jürgen F. H., Aram Mikaelyan, Tanja Woyke, and Andreas Brune. "Genome analysis of ‘Candidatus Ancillula trichonymphae’, first representative of a deep-branching clade of Bifidobacteriales , strengthens evidence for convergent evolution in flagellate endosymbionts." Environmental Microbiology Reports 8, no. 5 (2016): 865–73. http://dx.doi.org/10.1111/1758-2229.12451.

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Shen, Rui, Lixin Ke, Qiao Li, et al. "Abnormal bile acid-microbiota crosstalk promotes the development of hepatocellular carcinoma." Hepatology International 16, no. 2 (2022): 396–411. http://dx.doi.org/10.1007/s12072-022-10299-7.

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Abstract Background Gut microbiota and microbe-derived metabolites are involved in the development of HCC. Bile acids (BAs) are the most important gut microbiota-modulated endogenous signaling molecules. Methods We tested serum bile acid levels and gut microbiome compositions in patients with HCC, chemical-induced HCC mouse models (DEN-HCC mice) and mouse orthotopic implanted liver tumor models with vancomycin treatment (vancomycin-treated mice). Then, we screened an important kind of HCC-related BAs, and verified its effect on the growth of HCC in vivo and in vitro. Results We found that the
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Chen, Jing, Yuying Yang, Ningning Yu, Wanxiao Sun, Yuanyuan Yang, and Mei Zhao. "Relationship between gut microbiome characteristics and the effect of nutritional therapy on glycemic control in pregnant women with gestational diabetes mellitus." PLOS ONE 17, no. 4 (2022): e0267045. http://dx.doi.org/10.1371/journal.pone.0267045.

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The purpose of this study was to explore the relationship between the characteristics of gut microbiome and the effect of medical nutrition therapy (MNT) on glycemic control in pregnant women with gestational diabetes mellitus (GDM). Seventy-four pregnant women newly diagnosed with GDM received MNT for one-week. The effect of glycemic control was evaluated by fasting and 2-hour postprandial blood glucose; and stool samples of pregnant women were collected to detect the gut microbiome before and after MNT. We used a nested case-control study design, with pregnant women with GDM who did not meet
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Elkomy, Hassan S., Ivan I. Kochish, Olga V. Myasnikova, and Elena А. Prosekova. "Microflora and histostructure of the intestine of laying hens under the influence of prebiotic “Butifour F”." Veterinariya, Zootekhniya i Biotekhnologiya 5, no. 102 (2022): 13–24. http://dx.doi.org/10.36871/vet.zoo.bio.202205002.

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The aim of the research is to study the effect of a prebiotic (Butifour F) on the microflora of chickens’ caecum and the histostructure of the jejunum and proximal portion of the caecum in laying hens of the Lohmann Brown cross. To solve this problem in the conditions of the vivarium of the International Laboratory of Molecular Genetics and Poultry Genomics, Department of Animal Hygiene and Poultry A. K. Danilova, MVA by K. I. Skryabin, this experiment was carried out. The diet of laying hens of the experimental groups differed from the control one by introducing a prebiotic (Butifour F) at do
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Wang, Xiaojing, Tong Wang, Qian Zhang, Li Xu, and Xinhua Xiao. "Dietary Supplementation with Inulin Modulates the Gut Microbiota and Improves Insulin Sensitivity in Prediabetes." International Journal of Endocrinology 2021 (June 29, 2021): 1–8. http://dx.doi.org/10.1155/2021/5579369.

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Aims. Accumulating evidence indicates gut microbiota dysbiosis is involved in metabolic disorders, including prediabetes. The prebiotic inulin has been frequently reported to exert beneficial effects on the host metabolism. Here, we aimed to evaluate whether dietary supplementation with inulin modulates gut microbiota structure in prediabetes, affecting glucose and lipid metabolism. Methods. We performed a prospective single-arm study. A total of 49 subjects with prediabetes (WHO 1999 criteria) were voluntarily enrolled. Each subject received a daily supplement with 15 g of inulin for 6 months
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Eisenhauer, Laura, Wilfried Vahjen, Temesgen Dadi, Barbara Kohn, and Jürgen Zentek. "Effects of Brewer’s spent grain and carrot pomace on digestibility, fecal microbiota, and fecal and urinary metabolites in dogs fed low- or high-protein diets1." Journal of Animal Science 97, no. 10 (2019): 4124–33. http://dx.doi.org/10.1093/jas/skz264.

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Abstract Brewer’s spent grain (BSG) and carrot pomace (CAP) were used as fiber sources in low- or high-protein diets in dogs. Ten adult Beagles were involved in 5 feeding periods of 19 d in a cross-over design. Experimental diets contained 7.5% of total dietary fiber (TDF) from BSG or CAP and 20% or 40% of crude protein in dry matter. A diet with 3.5% TDF from both fiber sources and 20% crude protein was used as reference. Fecal dry matter was 27% higher for diets with BSG compared to CAP (P < 0.001). Apparent fecal digestibility of crude protein was 7% to 11% higher in diets with 40% prote
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Gorlov, Ivan F., Zoya B. Komarova, Marina I. Slozhenkina, et al. "Productive Performance, Hatching Egg Quality and Health Indices of Hisex Brown Laying Hens Fed Extruded Grain Amaranth." Basrah Journal of Agricultural Sciences 37, no. 1 (2024): 183–95. http://dx.doi.org/10.37077/25200860.2024.37.1.14.

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The article presents results proving the feasibility of extruded amaranth grain (Amaranthus cruentus) in feeding breeding laying hens of the Hisex Brown cross (aged 25-45 weeks). Due to improvements in the quality indicators of hatching eggs, such as weight (2.09%; P<0.01), Haugh units (1.07%; P<0.05), and shell thickness strengthened (5.0 µm; P<0.05) to 362 µm, it was possible to increase egg laying intensity by 1.81%, hatching egg yield by 2.20%, and hatching of chickens by 1.33% with the addition of 5% extruded grain to the diet structure. In the test group, there was an 11.66% (P&
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Sen, Arnab, Vincent Daubin, Danis Abrouk, Isaac Gifford, Alison M. Berry, and Philippe Normand. "Phylogeny of the class Actinobacteria revisited in the light of complete genomes. The orders ‘Frankiales’ and Micrococcales should be split into coherent entities: proposal of Frankiales ord. nov., Geodermatophilales ord. nov., Acidothermales ord. nov. and Nakamurellales ord. nov." International Journal of Systematic and Evolutionary Microbiology 64, Pt_11 (2014): 3821–32. http://dx.doi.org/10.1099/ijs.0.063966-0.

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The phylogeny of the class Actinobacteria remains controversial, essentially because it is very sensitive to the choice of dataset and phylogenetic methods. We used a test proposed recently, based on complete genome data, which chooses among candidate species phylogenies based on the number of lateral gene transfers (LGT) needed to explain the diversity of histories among gene trees for a set of genomes. We used 100 completely sequenced genomes representing 35 families and 17 orders of the class Actinobacteria and evaluated eight different hypotheses for their phylogeny, including one based on
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Duan, Xia, Jingjing Xu, Ping Yang, et al. "The effects of a set amount of regular maternal exercise during pregnancy on gut microbiota are diet-dependent in mice and do not cause significant diversity changes." PeerJ 10 (December 2, 2022): e14459. http://dx.doi.org/10.7717/peerj.14459.

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Background Diet and exercise can affect the gut microbiota (GM); however, the effects of the same amount of exercise on gut microbiota changes in people on a low-fat diet (LFD) and high-fat diet (HFD) during pregnancy are unknown. Do different nutritional conditions respond equally to exercise intervention? This study aimed to investigate the effects of regular maternal exercise during pregnancy on the GM in mice fed different diets during pregnancy. Methods Six-week-old nulliparous female KunMing mice were fed either a HFD or LFD before and during pregnancy. Each group of mice were then rando
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Zhang, Jianghui, Yuan Wang, Guimei Chen, et al. "The Associations between Multiple Essential Metal(loid)s and Gut Microbiota in Chinese Community-Dwelling Older Adults." Nutrients 15, no. 5 (2023): 1137. http://dx.doi.org/10.3390/nu15051137.

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Several experimental studies have suggested that individual essential metal(loid)s (EMs) could regulate the gut microbiota. However, human studies assessing the associations between EMs and gut microbiota are limited. This study aimed to examine the associations of individual and multiple EMs with the compositions of the gut microbiota in older adults. A total of 270 Chinese community-dwelling people over 60 years old were included in this study. Urinary concentrations of selected EMs, including vanadium (V), cobalt (Co), selenium (Se), strontium (Sr), magnesium (Mg), calcium (Ca), and molybde
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Li, Zhaolong, Wenjing Liu, Huini Wu, et al. "Effects of Lactiplantibacillus-plantarum-ZG7-Fermented Feed on Laying-Hen Productivity and Intestinal Health." Fermentation 11, no. 3 (2025): 123. https://doi.org/10.3390/fermentation11030123.

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The improvement in poultry production performance varies with different microbial strains used in fermented feed. This study investigates the efficacy of Lactiplantibacillus-plantarum-ZG7-fermented feed (ZG7-FF) on the productivity of laying hens. Results indicated that ZG7-FF significantly reduced the daily feed intake while increasing egg weight and decreasing the feed-to-egg ratio during peak production (p < 0.05), in addition to enhancing the late-phase laying rate (p < 0.05). Further intestinal morphological results showed that ZG7-FF significantly increased the length of villi in e
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Colbert, Lauren Elizabeth, Andrea Delgado Medrano, Rebecca A. Previs, et al. "Association of changes in vaginal microbiome with oligoclonal T-cell expansion and early response to chemoradiation for cervical cancer." Journal of Clinical Oncology 36, no. 5_suppl (2018): 8. http://dx.doi.org/10.1200/jco.2018.36.5_suppl.8.

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8 Background: The composition of the vaginal microbiome has been shown to affect clearance of HPV virus and transformation to invasive cancer. Clinical studies correlating the vaginal microbiome with immune activation and response to cancer treatment are lacking. We profiled intratumoral T-cell clonality during radiation therapy and correlated it with the diversity of the vaginal flora. Methods: Thirty patients with newly diagnosed locally advanced cervical cancer were enrolled on a prospective study to characterize changes in the cervical microbiome during chemoradiation. Cervical samples wer
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Turroni, Francesca, Elena Foroni, Paola Pizzetti, et al. "Exploring the Diversity of the Bifidobacterial Population in the Human Intestinal Tract." Applied and Environmental Microbiology 75, no. 6 (2009): 1534–45. http://dx.doi.org/10.1128/aem.02216-08.

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ABSTRACT Although the health-promoting roles of bifidobacteria are widely accepted, the diversity of bifidobacteria among the human intestinal microbiota is still poorly understood. We performed a census of bifidobacterial populations from human intestinal mucosal and fecal samples by plating them on selective medium, coupled with molecular analysis of selected rRNA gene sequences (16S rRNA gene and internally transcribed spacer [ITS] 16S-23S spacer sequences) of isolated colonies. A total of 900 isolates were collected, of which 704 were shown to belong to bifidobacteria. Analyses showed that
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Bottacini, Francesca, Duccio Medini, Angelo Pavesi, et al. "Comparative genomics of the genus Bifidobacterium." Microbiology 156, no. 11 (2010): 3243–54. http://dx.doi.org/10.1099/mic.0.039545-0.

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Whole-genome sequencing efforts have revolutionized the study of bifidobacterial genetics and physiology. Unfortunately, the sequence of a single genome does not provide information on bifidobacterial genetic diversity and on how genetic variability supports improved adaptation of these bacteria to the environment of the human gastrointestinal tract (GIT). Analysis of nine genomes from bifidobacterial species showed that such genomes display an open pan-genome structure. Mathematical extrapolation of the data indicates that the genome reservoir available to the bifidobacterial pan-genome consi
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Saturio, Silvia, Marta Suárez, Leonardo Mancabelli, et al. "Effect of Intrapartum Antibiotics Prophylaxis on the Bifidobacterial Establishment within the Neonatal Gut." Microorganisms 9, no. 9 (2021): 1867. http://dx.doi.org/10.3390/microorganisms9091867.

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Antibiotics are important disruptors of the intestinal microbiota establishment, linked to immune and metabolic alterations. The intrapartum antibiotics prophylaxis (IAP) is a common clinical practice that is present in more than 30% of labours, and is known to negatively affect the gut microbiota composition. However, little is known about how it affects to Bifidobacterium (sub)species level, which is one of the most important intestinal microbial genera early in life. This study presents qualitative and quantitative analyses of the bifidobacterial (sub)species populations in faecal samples,
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Van Limbergen, J., K. Dunn, E. Wine, R. Sigall Boneh, J. Bielawski, and A. Levine. "OP22 Crohn’s disease exclusion diet reduces bacterial dysbiosis towards healthy controls in paediatric Crohn’s disease." Journal of Crohn's and Colitis 14, Supplement_1 (2020): S019—S020. http://dx.doi.org/10.1093/ecco-jcc/jjz203.021.

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Abstract Background Dietary therapy with the Crohn’s disease Exclusion Diet (CDED) or Exclusive Enteral Nutrition (EEN) induces remission, reduces inflammation and is associated with compositional changes in the microbiome. We performed metagenome analysis to elucidate whether diet induces remission via correction of dysbiosis and/or community structure. Methods Whole metagenome and 16S rDNA sequences were obtained in 178 samples from 70 participants at 3 time points (baseline, week 6, week 12) from the two study groups (CDED+PEN/EEN). For metagenome analysis, we divided the groups further int
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Hsu, Clare, Fabio Marx, Ryan Guldenpfennig, Negin Valizadegan, and Maria R. C. de Godoy. "196 The Effect of Hydrolyzed Protein on Fecal Microbiota in Adult Dogs." Journal of Animal Science 101, Supplement_3 (2023): 109–10. http://dx.doi.org/10.1093/jas/skad281.134.

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Abstract Research on protein hydrolysates has observed various properties and functionalities of these ingredients depending on the type of hydrolysate. The objective of this study was to evaluate the effect of hydrolyzed chicken protein that was incorporated into diets on gut health in healthy adult dogs. Five complete and balanced treatment diets were manufactured: 1) CONd: chicken meal diet; 2) 5% CLHd: 5% substitution of chicken liver hydrolysate of chicken meal diet; 3) CLHd: chicken liver hydrolysate diet; 4) 5% CHd: 5% substitution of chicken hydrolysate of chicken meal diet; and 5) CHd
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Buckley, Darren, Toshitaka Odamaki, Jinzhong Xiao, Jennifer Mahony, Douwe van Sinderen, and Francesca Bottacini. "Diversity of Human-Associated Bifidobacterial Prophage Sequences." Microorganisms 9, no. 12 (2021): 2559. http://dx.doi.org/10.3390/microorganisms9122559.

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Members of Bifidobacterium play an important role in the development of the immature gut and are associated with positive long-term health outcomes for their human host. It has previously been shown that intestinal bacteriophages are detected within hours of birth, and that induced prophages constitute a significant source of such gut phages. The gut phageome can be vertically transmitted from mother to newborn and is believed to exert considerable selective pressure on target prokaryotic hosts affecting abundance levels, microbiota composition, and host characteristics. The objective of the c
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Stewart, Christopher J. "Breastfeeding promotes bifidobacterial immunomodulatory metabolites." Nature Microbiology 6, no. 11 (2021): 1335–36. http://dx.doi.org/10.1038/s41564-021-00975-z.

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Matsuki, Takahiro, Koichi Watanabe, Ryuichiro Tanaka, Masafumi Fukuda, and Hiroshi Oyaizu. "Distribution of Bifidobacterial Species in Human Intestinal Microflora Examined with 16S rRNA-Gene-Targeted Species-Specific Primers." Applied and Environmental Microbiology 65, no. 10 (1999): 4506–12. http://dx.doi.org/10.1128/aem.65.10.4506-4512.1999.

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ABSTRACT In order to clarify the distribution of bifidobacterial species in the human intestinal tract, a 16S rRNA-gene-targeted species-specific PCR technique was developed and used with DNAs extracted from fecal samples obtained from 48 healthy adults and 27 breast-fed infants. To cover all of the bifidobacterial species that have been isolated from and identified in the human intestinal tract, species-specific primers for Bifidobacterium longum, B. infantis,B. dentium, and B. gallicum were developed and used with primers for B. adolescentis, B. angulatum, B. bifidum, B. breve, and the B. ca
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Mancino, Walter, Sabrina Duranti, Leonardo Mancabelli, et al. "Bifidobacterial Transfer from Mother to Child as Examined by an Animal Model." Microorganisms 7, no. 9 (2019): 293. http://dx.doi.org/10.3390/microorganisms7090293.

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Bifidobacteria commonly constitute the most abundant group of microorganisms in the healthy infant gut. Their intestinal establishment is believed to be maternally driven, and their acquisition has even been postulated to occur during pregnancy. In the current study, we evaluated bifidobacterial mother-to infant transmission events in a rat model by means of quantitative PCR (qPCR), as well as by Internally Transcribed Spacer (ITS) bifidobacterial profiling. The occurrence of strains supplied by mothers during pregnancy to their corresponding newborns was observed and identified by analysis im
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Xu, Shanrong, Wenqi Liu, Li Gong, et al. "Association of ADRB2 gene polymorphisms and intestinal microbiota in Chinese Han adolescents." Open Life Sciences 18, no. 1 (2023). http://dx.doi.org/10.1515/biol-2022-0646.

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Abstract Gut microbiota are closely related to health, and the β2-adrenergic receptor (ADRB2) gene is associated with gastrointestinal diseases. However, little is known about the relationship between ADRB2 gene polymorphisms and intestinal microbiota. In the present study, we aimed to explore the relationship between ADRB2 gene polymorphisms and gut microbiota in Chinese Han adolescents. Data analysis showed that the relative abundance, PICRUSt function prediction, and Chao1 and ACE indices of gut microbiota were significantly different between males and females (P < 0.05). The rs1042711 w
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Zhang, Grace, Beile Gao, Mobolaji Adeolu, Bijendra Khadka, and Radhey S. Gupta. "Phylogenomic Analyses and Comparative Studies on Genomes of the Bifidobacteriales: Identification of Molecular Signatures Specific for the Order Bifidobacteriales and Its Different Subclades." Frontiers in Microbiology 7 (June 27, 2016). http://dx.doi.org/10.3389/fmicb.2016.00978.

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"ОТ НАУКИ К ПРАКТИКЕ: РАЦИОНАЛЬНЫЙ ПОДХОД К КОНТРОЛЮ МИКРОФЛОРЫ КИШЕЧНИКА ПТИЦЫ Кочиш И.И., Мясникова О.В., Никонов И.Н., Худяков А.А." Птицеводство 72, № 1 (2023). http://dx.doi.org/10.33845/0033-3239-2023-72-1-39-42.

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Одной из актуальных для современного птицеводства задач является создание инновационных продуктов для коррекции кишечного микробиома у сельскохозяйственной птицы в условиях промышленного содержания, обладающих высокой антагонистической активностью к распространенным энтеропатогенам. Были проведены производственные испытания на цыплятах-бройлерах кросса Росс-308 бактериального пробиотика Бактосель (Lallemand Animal Nutrition) на основе молочнокислых бактерий Pediococcus acidilactici, который выпаивали опытной группе взамен антибиотика флорфеникол 10% (выпаивавшегося контрольной группе) в дозиро
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Höyhtyä, Miikka, Katri Korpela, Schahzad Saqib та ін. "Quantitative Fecal Microbiota Profiles Relate to Therapy Response During Induction With Tumor Necrosis Factor α Antagonist Infliximab in Pediatric Inflammatory Bowel Disease". Inflammatory Bowel Diseases, 30 серпня 2022. http://dx.doi.org/10.1093/ibd/izac182.

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Abstract Background The role of intestinal microbiota in inflammatory bowel diseases is intensively researched. Pediatric studies on the relation between microbiota and treatment response are sparse. We aimed to determine whether absolute abundances of gut microbes characterize the response to infliximab induction in pediatric inflammatory bowel disease. Methods We recruited pediatric patients with inflammatory bowel disease introduced to infliximab at Children’s Hospital, University of Helsinki. Stool samples were collected at 0, 2, and 6 weeks for microbiota and calprotectin analyses. We def
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Backer Mortensen, Trude. "Ultra-Processed Foods and the Intestinal Bacterial Flora?" Kompass Nutrition & Dietetics, April 1, 2022, 1–2. http://dx.doi.org/10.1159/000522430.

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Ultra-processed foods (UPFs) consumption could affect gut microbiota diversity and profile. We aimed to evaluate the effects of UPFs on microbiota, considering the role of sex. The consumption of UPFs (using NOVA criteria) was assessed with a validated 137-item food-frequency questionnaire. Participants (<i>n</i> = 359) were classified into less than three servings per day (<i>n</i> = 96) of UPFs and more than five (<i>n</i> = 90). Women and men were subclassified following the same criteria. 16S rRNA sequencing was performed from DNA fecal samples, and diff
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Qu, Yang, Yan Wu, Wei Cheng, et al. "Amelioration of cognitive impairment using epigallocatechin-3-gallate in ovariectomized mice fed a high-fat diet involves remodeling with Prevotella and Bifidobacteriales." Frontiers in Pharmacology 13 (January 4, 2023). http://dx.doi.org/10.3389/fphar.2022.1079313.

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Background: Estrogen deficiency and a high-fat diet (HFD) are both risk factors for Alzheimer’s disease (AD). HFD can accelerate cognitive impairment in estrogen-deficient patients, but there is currently no effective treatment. Epigallocatechin-3-galate (EGCG) is widely studied for its anti-inflammatory, anti-cancer, and anti-neurodegeneration effects. Nevertheless, whether EGCG can ameliorate cognitive impairment in HFD-fed estrogen-deficient mice has not been studied.Methods and Results: Ovariectomized (OVX) mice fed an HFD (HFOVX) for 8 weeks experienced impaired object recognition and spa
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"ВЛИЯНИЕ ПРЕБИОТИКА НА ОСНОВЕ БУТИРАТА НА МИКРОФЛОРУ КИШЕЧНИКА И ЭКСПРЕССИЮ ГЕНОВ РЕЗИСТЕНТНОСТИ У КУР-НЕСУШЕК КРОССА ЛОМАНН БРАУН Кочиш И.И., Элькоми Х.С., Мясникова О.В., Брылин А.П." Птицеводство 71, № 3 (2022). http://dx.doi.org/10.33845/0033-3239-2022-71-3-49-54.

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Изучено влияние бутират-содержащего пребиотического препарата Бутифор F на количественный и качественный состав микробиоты слепых отростков кишечника и экспрессию генов, связанных с резистентностью, у кур-несушек кросса Ломанн Браун. Было обнаружено, что добавление пребиотика к корму (750 г/т) приводило к достоверному увеличению в слепых отростках численности бактерий филума Actinobacteria в 3,5 раза, в т.ч. порядка Bifidobacteriales - в 2,7 раза (P<0,05), увеличению численности бактерий семейства Lactobacillaceae на 135% (P<0,05) и снижению общего количества патогенной и нежелательной м
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Ma, Yong, Yanquan Fei, Xuebing Han, Gang Liu, and Jun Fang. "Lactobacillus plantarum Alleviates Obesity by Altering the Composition of the Gut Microbiota in High-Fat Diet-Fed Mice." Frontiers in Nutrition 9 (June 30, 2022). http://dx.doi.org/10.3389/fnut.2022.947367.

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Metabolic disorders and intestinal flora imbalance usually accompany obesity. Due to its diverse biological activities, Lactobacillus plantarum is widely used to alleviate various diseases as a probiotic. Here, we show that L. plantarum can reduce the body weight of mice fed high-fat diets, reduce fat accumulation, and enhance mice glucose tolerance. Our results show that L. plantarum can significantly reduce the expression of DGAT1 and DGAT2, increase the expression of Cpt1a, and promote the process of lipid metabolism. Further data show that L. plantarum can increase the SCFA content in the
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Ren, Yuan, Yao Zhang, Yanan Cheng, Hao Qin, and Hui Zhao. "Genetic liability of gut microbiota for idiopathic pulmonary fibrosis and lung function: a two-sample Mendelian randomization study." Frontiers in Cellular and Infection Microbiology 14 (May 22, 2024). http://dx.doi.org/10.3389/fcimb.2024.1348685.

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BackgroundThe microbiota-gut-lung axis has elucidated a potential association between gut microbiota and idiopathic pulmonary fibrosis (IPF). However, there is a paucity of population-level studies with providing robust evidence for establishing causality. This two-sample Mendelian randomization (MR) analysis aimed to investigate the causal relationship between the gut microbiota and IPF as well as lung function.Materials and methodsAdhering to Mendel’s principle of inheritance, this MR analysis utilized summary-level data from respective genome-wide association studies (GWAS) involving 211 gu
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Song, Xinni, Xinyue Fu, Shufang Niu, et al. "Exploring the Effects of Saorilao-4 on the Gut Microbiota of Pulmonary Fibrosis Model Rats Based on 16S rRNA Sequencing." Journal of Applied Microbiology, July 17, 2024. http://dx.doi.org/10.1093/jambio/lxae178.

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Abstract Aims Pulmonary fibrosis (PF) is a progressive and incurable lung disease for which treatment options are limited. Here, we aimed to conduct an exploratory study on the effects of the Mongolian medicine Saorilao-4 (SRL) on the gut microbiota structure, species abundance, and diversity of a rat PF model as well as the mechanisms underlying such effects. Methods and results Rat fecal samples were analyzed using 16S rRNA sequencing technology. Bioinformatic and correlation analyses were performed on microbiota data to determine significant associations. SRL substantially attenuated the ad
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Hotchkiss, Michelle Z., Alexandre J. Poulain, and Jessica R. K. Forrest. "Pesticide-induced disturbances of bee gut microbiotas." FEMS Microbiology Reviews 46, no. 2 (2022). http://dx.doi.org/10.1093/femsre/fuab056.

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ABSTRACT Social bee gut microbiotas play key roles in host health and performance. Worryingly, a growing body of literature shows that pesticide exposure can disturb these microbiotas. Most studies examine changes in taxonomic composition in Western honey bee (Apis mellifera) gut microbiotas caused by insecticide exposure. Core bee gut microbiota taxa shift in abundance after exposure but are rarely eliminated, with declines in Bifidobacteriales and Lactobacillus near melliventris abundance being the most common shifts. Pesticide concentration, exposure duration, season and concurrent stressor
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Zhu, Zhihui, Jianghui Zhang, Guojing Yuan, et al. "Association between mobile phone addiction, sleep disorder and the gut microbiota: a short-term prospective observational study." Frontiers in Microbiology 14 (December 19, 2023). http://dx.doi.org/10.3389/fmicb.2023.1323116.

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Bidirectional communication between the gut microbiota and the brain has sparked interest in exploring the link between mobile phone addiction (MPA) and sleep disorders (SD) in microbiome research. However, investigating the role of gut microbiota in this relationship using animal models presents challenges due to the unique nature of MPA, and human research in this area is scarce. We recruited 99 healthy college students to evaluate the gut microbiome using 16S rRNA gene amplicon sequencing and assess MPA and SD at baseline and after a two-month follow-up. Multiple covariate-adjusted statisti
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Kato, Satsuki, Toshiyuki Nagasawa, Osamu Uehara, et al. "Increase in Bifidobacterium is a characteristic of the difference in the salivary microbiota of pregnant and non-pregnant women." BMC Oral Health 22, no. 1 (2022). http://dx.doi.org/10.1186/s12903-022-02293-0.

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Abstract Background The establishment of symbiotic microbiota in pregnant women is important for both the mother and her offspring. Little is known about the salivary symbiotic bacteria in pregnancy, and analysis of composition of microbiome (ANCOM) is useful to detect small differences in the number of bacteria. The aim of this study was to investigate the differences in the salivary bacteria between healthy pregnant and non-pregnant women using ANCOM. Methods Unstimulated saliva samples were collected from 35 healthy pregnant women at 35 weeks gestation and 30 healthy non-pregnant women duri
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