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Artykuły w czasopismach na temat „Bifidobacterium infantis”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 25 maja 2024

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1

Sakurai, Takuma, Toshitaka Odamaki, and Jin-zhong Xiao. "Production of Indole-3-Lactic Acid by Bifidobacterium Strains Isolated fromHuman Infants." Microorganisms 7, no. 9 (September 11, 2019): 340. http://dx.doi.org/10.3390/microorganisms7090340.

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Recent studies have shown that metabolites produced by microbes can be considered as mediators of host-microbial interactions. In this study, we examined the production of tryptophan metabolites by Bifidobacterium strains found in the gastrointestinal tracts of humans and other animals. Indole-3-lactic acid (ILA) was the only tryptophan metabolite produced in bifidobacteria culture supernatants. No others, including indole-3-propionic acid, indole-3-acetic acid, and indole-3-aldehyde, were produced. Strains of bifidobacterial species commonly isolated from the intestines of human infants, such

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Chen, Yuan Yao, Xin Zhao, Wolfgang Moeder, Hein M. Tun, Elinor Simons, Piushkumar J. Mandhane, Theo J. Moraes, et al. "Impact of Maternal Intrapartum Antibiotics, and Caesarean Section with and without Labour on Bifidobacterium and Other Infant Gut Microbiota." Microorganisms 9, no. 9 (August 31, 2021): 1847. http://dx.doi.org/10.3390/microorganisms9091847.

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Background and Aims: Few studies consider the joint effect of multiple factors related to birth, delivery mode, intrapartum antibiotic prophylaxis and the onset of labour, on the abundance of Bifidobacterium and the quantity of this genus and its species Bifidobacterium longum subsp. infantis in the infant gut microbiota. We implemented such a study. Methods: Among 1654 Canadian full-term infants, the gut microbiota of faecal samples collected at 3 months were profiled by 16S rRNA sequencing; the genus Bifidobacterium and Bifidobacterium longum subsp. infantis were quantified by qPCR. Associat

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Young, Sarah L., Mary A. Simon, Margaret A. Baird, Gerald W. Tannock, Rodrigo Bibiloni, Kate Spencely, Juliette M. Lane, et al. "Bifidobacterial Species Differentially Affect Expression of Cell Surface Markers and Cytokines of Dendritic Cells Harvested from Cord Blood." Clinical Diagnostic Laboratory Immunology 11, no. 4 (July 2004): 686–90. http://dx.doi.org/10.1128/cdli.11.4.686-690.2004.

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ABSTRACT The gut microbiota may be important in the postnatal development of the immune system and hence may influence the prevalence of atopic diseases. Bifidobacteria are the most numerous bacteria in the guts of infants, and the presence or absence of certain species could be important in determining the geographic incidence of atopic diseases. We compared the fecal populations of bifidobacteria from children aged 25 to 35 days in Ghana (which has a low prevalence of atopy), New Zealand, and the United Kingdom (high-prevalence countries). Natal origin influenced the detection of bifidobacte

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4

Sakurai, T., A. Yamada, N. Hashikura, T. Odamaki, and J. Z. Xiao. "Degradation of food-derived opioid peptides by bifidobacteria." Beneficial Microbes 9, no. 4 (June 15, 2018): 675–82. http://dx.doi.org/10.3920/bm2017.0165.

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Some food-derived opioid peptides have been reported to cause diseases, such as gastrointestinal inflammation, celiac disease, and mental disorders. Bifidobacterium is a major member of the dominant human gut microbiota, particularly in the gut of infants. In this study, we evaluated the potential of Bifidobacterium in the degradation of food-derived opioid peptides. All strains tested showed some level of dipeptidyl peptidase activity, which is thought to be involved in the degradation of food-derived opioid peptides. However, this activity was higher in bifidobacterial strains that are commo

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5

Hiraku, Akari, Setsuko Nakata, Mai Murata, Chendong Xu, Natsumi Mutoh, Satoshi Arai, Toshitaka Odamaki, et al. "Early Probiotic Supplementation of Healthy Term Infants with Bifidobacterium longum subsp. infantis M-63 Is Safe and Leads to the Development of Bifidobacterium-Predominant Gut Microbiota: A Double-Blind, Placebo-Controlled Trial." Nutrients 15, no. 6 (March 14, 2023): 1402. http://dx.doi.org/10.3390/nu15061402.

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Bifidobacteria are important intestinal bacteria that provide a variety of health benefits in infants. We investigated the efficacy and safety of Bifidobacterium longum subsp. infantis (B. infantis) M-63 in healthy infants in a double-blind, randomized, placebo-controlled trial. Healthy term infants were given B. infantis M-63 (n = 56; 1 × 109 CFU/day) or placebo (n = 54) from postnatal age ≤ 7 days to 3 months. Fecal samples were collected, and fecal microbiota, stool pH, short-chain fatty acids, and immune substances were analyzed. Supplementation with B. infantis M-63 significantly increase

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6

Pozo-Rubio, T., J. R. Mujico, A. Marcos, E. Puertollano, I. Nadal, Y. Sanz, and E. Nova. "Immunostimulatory effect of faecal Bifidobacterium species of breast-fed and formula-fed infants in a peripheral blood mononuclear cell/Caco-2 co-culture system." British Journal of Nutrition 106, no. 8 (May 31, 2011): 1216–23. http://dx.doi.org/10.1017/s0007114511001656.

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Bifidobacterium spp. typical of the human intestinal microbiota are believed to influence the balance of immune responses in the intestinal mucosa. The aim of the present study was to investigate the effect of different bifidobacterial species and their mixtures in in vitro experiments with peripheral blood mononuclear cells (PBMC) and Caco-2 cells. Bifidobacterium adolescentis, B. angulatum, B. breve, B. catenulatum, B. infantis, B. longum and two combinations of these bifidobacteria simulating the species composition found in faecal samples from breast-fed (BF) and formula-fed (FF) infants w

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7

Hilliard, Margaret A., and David A. Sela. "Transmission and Persistence of Infant Gut-Associated Bifidobacteria." Microorganisms 12, no. 5 (April 27, 2024): 879. http://dx.doi.org/10.3390/microorganisms12050879.

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Bifidobacterium infantis are the primary colonizers of the infant gut, yet scientific research addressing the transmission of the genus Bifidobacterium to infants remains incomplete. This review examines microbial reservoirs of infant-type Bifidobacterium that potentially contribute to infant gut colonization. Accordingly, strain inheritance from mother to infant via the fecal-oral route is likely contingent on the bifidobacterial strain and phenotype, whereas transmission via the vaginal microbiota may be restricted to Bifidobacterium breve. Additional reservoirs include breastmilk, horizonta

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8

Taft, Diana H., Zachery T. Lewis, Nhu Nguyen, Steve Ho, Chad Masarweh, Vanessa Dunne-Castagna, Daniel J. Tancredi, et al. "Bifidobacterium Species Colonization in Infancy: A Global Cross-Sectional Comparison by Population History of Breastfeeding." Nutrients 14, no. 7 (March 29, 2022): 1423. http://dx.doi.org/10.3390/nu14071423.

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Bifidobacterium species are beneficial and dominant members of the breastfed infant gut microbiome; however, their health benefits are partially species-dependent. Here, we characterize the species and subspecies of Bifidobacterium in breastfed infants around the world to consider the potential impact of a historic dietary shift on the disappearance of B. longum subsp. infantis in some populations. Across populations, three distinct patterns of Bifidobacterium colonization emerged: (1) The dominance of Bifidobacterium longum subspecies infantis, (2) prevalent Bifidobacterium of multiple specie

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9

Peirotén, A., J. L. Arqués, M. Medina, and E. Rodríguez-Mínguez. "Bifidobacterial strains shared by mother and child as source of probiotics." Beneficial Microbes 9, no. 2 (February 27, 2018): 231–38. http://dx.doi.org/10.3920/bm2017.0133.

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Importance of bifidobacteria as part of the infant intestinal microbiota has been highlighted. Their acquisition is influenced by the mode of birth and the feed regime afterwards, with a special role of the maternal microbiota. The presence of the same shared bifidobacterial strains between breast milk and infant faeces in 14 mother-infant pairs was assessed by means of pulsed-field gel electrophoresis (PFGE) genotyping. Four shared strains of Bifidobacterium breve (2), Bifidobacterium longum subsp. infantis and B. longum subsp. longum were found in breast milk-infant faeces pairs. Two years l

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10

Haarman, Monique, and Jan Knol. "Quantitative Real-Time PCR Assays To Identify and Quantify Fecal Bifidobacterium Species in Infants Receiving a Prebiotic Infant Formula." Applied and Environmental Microbiology 71, no. 5 (May 2005): 2318–24. http://dx.doi.org/10.1128/aem.71.5.2318-2324.2005.

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ABSTRACT A healthy intestinal microbiota is considered to be important for priming of the infants' mucosal and systemic immunity. Breast-fed infants typically have an intestinal microbiota dominated by different Bifidobacterium species. It has been described that allergic infants have different levels of specific Bifidobacterium species than healthy infants. For the accurate quantification of Bifidobacterium adolescentis, Bifidobacterium angulatum, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium catenulatum, Bifidobacterium dentium, Bifidobacterium infantis, and Bifidobacterium

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11

Bozkurt, Hüseyin. "PROBIOTIC BIFIDOBACTERIUM AND A NEW GENERATION POSTBIOTIC: ALGINIC ACID." Inflammatory Bowel Diseases 29, Supplement_1 (January 26, 2023): S48. http://dx.doi.org/10.1093/ibd/izac247.091.

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Abstract AIMS OF THE STUDY According to International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus (2019),Postbiotics are defined as a preparation of inanimate microorganisms and/or their components that confers a health benefit on the host. Postbiotic Alginic acid, also called algin, is a naturally occurring, edible exopolysaccharide found in brown algae. Alginic acid and its Na/Ca salt form of Alginate show the capability in water-binding, water-retention water, and immense swelling and gelation, which could act as a protective barrier via promoting biofilm formatio

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12

Wong, Chyn Boon, Huidong Huang, Yibing Ning, and Jinzhong Xiao. "Probiotics in the New Era of Human Milk Oligosaccharides (HMOs): HMO Utilization and Beneficial Effects of Bifidobacterium longum subsp. infantis M-63 on Infant Health." Microorganisms 12, no. 5 (May 17, 2024): 1014. http://dx.doi.org/10.3390/microorganisms12051014.

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A healthy gut microbiome is crucial for the immune system and overall development of infants. Bifidobacterium has been known to be a predominant species in the infant gut; however, an emerging concern is the apparent loss of this genus, in particular, Bifidobacterium longum subsp. infantis (B. infantis) in the gut microbiome of infants in industrialized nations, underscoring the importance of restoring this beneficial bacterium. With the growing understanding of the gut microbiome, probiotics, especially infant-type human-residential bifidobacteria (HRB) strains like B. infantis, are gaining p

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13

Yang, Bo, Yingqi Chen, Catherine Stanton, R. Paul Ross, Yuan-Kun Lee, Jianxin Zhao, Hao Zhang, and Wei Chen. "Bifidobacterium and Lactobacillus Composition at Species Level and Gut Microbiota Diversity in Infants before 6 Weeks." International Journal of Molecular Sciences 20, no. 13 (July 5, 2019): 3306. http://dx.doi.org/10.3390/ijms20133306.

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Our objective was to investigate the effects of different delivery and feeding modes on the gut microbiota composition of early infants with special emphasis on Bifidobacterium and Lactobacillus profiles at species level. 16S rRNA V3-V4 regions, bifidobacterial, and lactobacilli groEL genes from infant feces were sequenced by Illumina MiSeq. Gut microbiota abundance was significantly different, where standard vaginally delivered (SVD) and breast-fed (BF) groups were higher in comparison with caesarean section (CS), milk-powder-fed (MPF), and mixed-fed (MF) groups. The genus unclassified Entero

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14

Roger, Laure C., Adele Costabile, Diane T. Holland, Lesley Hoyles, and Anne L. McCartney. "Examination of faecal Bifidobacterium populations in breast- and formula-fed infants during the first 18 months of life." Microbiology 156, no. 11 (November 1, 2010): 3329–41. http://dx.doi.org/10.1099/mic.0.043224-0.

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Bifidobacteria in the infant faecal microbiota have been the focus of much interest, especially during the exclusive milk-feeding period and in relation to the fortification of infant formulae to better mimic breast milk. However, longitudinal studies examining the diversity and dynamics of the Bifidobacterium population of infants are lacking, particularly in relation to the effects of weaning. Using a polyphasic strategy, the Bifidobacterium populations of breast- and formula-fed infants were examined during the first 18 months of life. Bifidobacterium-specific denaturing gradient gel electr

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15

Abdelazez, Amro, Zafarullah Muhammad, Qiu-Xue Zhang, Zong-Tao Zhu, Heba Abdelmotaal, Rokayya Sami, and Xiang-Chen Meng. "Production of a Functional Frozen Yogurt Fortified with Bifidobacterium spp." BioMed Research International 2017 (2017): 1–10. http://dx.doi.org/10.1155/2017/6438528.

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Frozen dairy products have characteristics of both yogurt and ice cream and could be the persuasive carriers of probiotics. Functions of the frozen yogurt containing viable bifidobacterial cells are recognized and favored by the people of all ages. We developed a kind of yogurt supplemented by Bifidobacterium species. Firstly, five strains of Bifidobacterium spp. (Bifidobacterium bifidum ATCC 11547, Bifidobacterium longum ATCC 11549, Bifidobacterium infantis ATCC 11551, Bifidobacterium adolescentis ATCC 11550, and Bifidobacterium breve ATCC 11548) were evaluated based on the feasibility criter

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Quinn, Erinn, Helen Slattery, Aoife Thompson, Michelle Kilcoyne, Lokesh Joshi, and Rita Hickey. "Mining Milk for Factors which Increase the Adherence of Bifidobacterium longum subsp. infantis to Intestinal Cells." Foods 7, no. 12 (December 3, 2018): 196. http://dx.doi.org/10.3390/foods7120196.

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Bifidobacteria play a vital role in human nutrition and health by shaping and maintaining the gut ecosystem. In order to exert a beneficial effect, a sufficient population of bifidobacteria must colonise the host. In this study, we developed a miniaturised high-throughput in vitro assay for assessing the colonising ability of bacterial strains in human cells. We also investigated a variety of components isolated from different milk sources for their ability to increase the adherence of Bifidobacterium longum subsp. infantis ATCC 15697, a common member of the gastrointestinal microbiota of brea

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Singh, Harsh Pratap, Pradip Kumar Sharma, Mahesh Kumar, and Parul Sharma. "Measuring the Growth of Lactobacillus fermentum and Bifidobacterium infantis in the presence of N-Acetyl L-Cysteine Hydrochloric Acid." International Journal of Medical and Allied Health Sciences 1, no. 02 (July 7, 2021): 49–55. http://dx.doi.org/10.54618/ijmahs.2021124.

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Aim: To study the growth of probiotic microorganisms including Lactobacillus fermentum and Bifidobacterium infantis in presence of N-Acetyl L-Cysteine Hydrochloric Acid (NACHCl) Settings and Design: Lactobacillus fermentum and Bifidobacterium infantis growth in the presence of N-Acetyl LCysteine Hydrochloric Acid were studied by using MRS broth without any additional supplement as a control. Material and Methods: Lactobacillus fermentum and Bifidobacterium infantis isolated from fecal samples of infants and adults were collected. Positive culture of Lactobacillus fermentum and Bifidobacterium

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Tsuji, H., R. Oozeer, K. Matsuda, T. Matsuki, T. Ohta, K. Nomoto, R. Tanaka, et al. "Molecular monitoring of the development of intestinal microbiota in Japanese infants." Beneficial Microbes 3, no. 2 (June 1, 2012): 113–25. http://dx.doi.org/10.3920/bm2011.0038.

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The faecal microbiota of 166 healthy Japanese newborns was analysed periodically from day 1 after birth until the age of 3 years by using the reverse transcription-quantitative PCR. Faecal pH and the organic acid concentration were also examined. Colonisation by both facultative anaerobes and strict anaerobes was confirmed in 95% of the meconium tested. Bifidobacterium-predominant microbiota was established subsequently in most of the infants by 3 months after birth. Bifidobacterium breve, Bifidobacterium longum, Bifidobacterium infantis, Bifidobacterium catenulatum group and Bifidobacterium b

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Sakata, S. "Unification of Bifidobacterium infantis and Bifidobacterium suis as Bifidobacterium longum." INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 52, no. 6 (November 1, 2002): 1945–51. http://dx.doi.org/10.1099/ijs.0.02221-0.

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Sakata, Shinji, Maki Kitahara, Mitsuo Sakamoto, Hidenori Hayashi, Masafumi Fukuyama, and Yoshimi Benno. "Unification of Bifidobacterium infantis and Bifidobacterium suis as Bifidobacterium longum." International Journal of Systematic and Evolutionary Microbiology 52, no. 6 (November 1, 2002): 1945–51. http://dx.doi.org/10.1099/00207713-52-6-1945.

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Woodmansey, Emma J., Marion E. T. McMurdo, George T. Macfarlane, and Sandra Macfarlane. "Comparison of Compositions and Metabolic Activities of Fecal Microbiotas in Young Adults and in Antibiotic-Treated and Non-Antibiotic-Treated Elderly Subjects." Applied and Environmental Microbiology 70, no. 10 (October 2004): 6113–22. http://dx.doi.org/10.1128/aem.70.10.6113-6122.2004.

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ABSTRACT The colonic microbiota mediates many cellular and molecular events in the host that are important to health. These processes can be affected in the elderly, because in some individuals, the composition and metabolic activities of the microbiota change with age. Detailed characterizations of the major groups of fecal bacteria in healthy young adults, in healthy elderly people, and in hospitalized elderly patients receiving antibiotics were made in this study, together with measurements of their metabolic activities, by analysis of fecal organic acid and ammonia concentrations. The resu

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Tso, Lauren, Kevin S. Bonham, Alyssa Fishbein, Sophie Rowland, and Vanja Klepac-Ceraj. "Targeted High-Resolution Taxonomic Identification of Bifidobacterium longum subsp. infantis Using Human Milk Oligosaccharide Metabolizing Genes." Nutrients 13, no. 8 (August 18, 2021): 2833. http://dx.doi.org/10.3390/nu13082833.

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Bifidobacterium longum subsp. infantis (B. infantis) is one of a few microorganisms capable of metabolizing human breast milk and is a pioneer colonizer in the guts of breastfed infants. One current challenge is differentiating B. infantis from its close relatives, B. longum and B. suis. All three organisms are classified in the same species group but only B. infantis can metabolize human milk oligosaccharides (HMOs). We compared HMO-metabolizing genes across different Bifidobacterium genomes and developed B. infantis-specific primers to determine if the genes alone or the primers can be used

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Chichlowski, Maciej, Neil Shah, Jennifer L. Wampler, Steven S. Wu, and Jon A. Vanderhoof. "Bifidobacterium longum Subspecies infantis (B. infantis) in Pediatric Nutrition: Current State of Knowledge." Nutrients 12, no. 6 (May 28, 2020): 1581. http://dx.doi.org/10.3390/nu12061581.

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Since originally isolated in 1899, the genus Bifidobacterium has been demonstrated to predominate in the gut microbiota of breastfed infants and to benefit the host by accelerating maturation of the immune response, balancing the immune system to suppress inflammation, improving intestinal barrier function, and increasing acetate production. In particular, Bifidobacterium longum subspecies infantis (B. infantis) is well adapted to the infant gut and has co-evolved with the mother-infant dyad and gut microbiome, in part due to its ability to consume complex carbohydrates found in human milk. B.

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Blanco, Guillermo, Lorena Ruiz, Hector Tamés, Patricia Ruas-Madiedo, Florentino Fdez-Riverola, Borja Sánchez, Anália Lourenço, and Abelardo Margolles. "Revisiting the Metabolic Capabilities of Bifidobacterium longum susbp. longum and Bifidobacterium longum subsp. infantis from a Glycoside Hydrolase Perspective." Microorganisms 8, no. 5 (May 13, 2020): 723. http://dx.doi.org/10.3390/microorganisms8050723.

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Bifidobacteria are among the most abundant microorganisms inhabiting the intestine of humans and many animals. Within the genus Bifidobacterium, several beneficial effects have been attributed to strains belonging to the subspecies Bifidobacterium longum subsp. longum and Bifidobacterium longum subsp. infantis, which are often found in infants and adults. The increasing numbers of sequenced genomes belonging to these two subspecies, and the availability of novel computational tools focused on predicting glycolytic abilities, with the aim of understanding the capabilities of degrading specific

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Mennini, Maurizio, Sofia Reddel, Federica Del Chierico, Simone Gardini, Andrea Quagliariello, Pamela Vernocchi, Rocco Luigi Valluzzi, et al. "Gut Microbiota Profile in Children with IgE-Mediated Cow’s Milk Allergy and Cow’s Milk Sensitization and Probiotic Intestinal Persistence Evaluation." International Journal of Molecular Sciences 22, no. 4 (February 6, 2021): 1649. http://dx.doi.org/10.3390/ijms22041649.

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Food allergy (FA) and, in particular, IgE-mediated cow’s milk allergy is associated with compositional and functional changes of gut microbiota. In this study, we compared the gut microbiota of cow’s milk allergic (CMA) infants with that of cow’s milk sensitized (CMS) infants and Healthy controls. The effect of the intake of a mixture of Bifidobacterium longum subsp. longum BB536, Bifidobacterium breve M-16V and Bifidobacterium longum subsp. infantis M-63 on gut microbiota modulation of CMA infants and probiotic persistence was also investigated. Gut microbiota of CMA infants resulted to be ch

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Duar, Rebbeca M., David Kyle, and Giorgio Casaburi. "Colonization Resistance in the Infant Gut: The Role of B. infantis in Reducing pH and Preventing Pathogen Growth." High-Throughput 9, no. 2 (March 27, 2020): 7. http://dx.doi.org/10.3390/ht9020007.

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Over the past century, there has been a steady increase in the stool pH of infants from industrialized countries. Analysis of historical data revealed a strong association between abundance of Bifidobacterium in the gut microbiome of breasted infants and stool pH, suggesting that this taxon plays a key role in determining the pH in the gut. Bifidobacterium longum subsp. infantis is uniquely equipped to metabolize human milk oligosaccharides (HMO) from breastmilk into acidic end products, mainly lactate and acetate. The presence of these acidic compounds in the infant gut is linked to a lower s

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Newton, Dorothy F., Sandra Macfarlane, and George T. Macfarlane. "Effects of Antibiotics on Bacterial Species Composition and Metabolic Activities in Chemostats Containing Defined Populations of Human Gut Microorganisms." Antimicrobial Agents and Chemotherapy 57, no. 5 (February 12, 2013): 2016–25. http://dx.doi.org/10.1128/aac.00079-13.

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ABSTRACTThe composition and metabolic activities of the human colonic microbiota are modulated by a number of external factors, including diet and antibiotic therapy. Changes in the structure and metabolism of the gut microbiota may have long-term consequences for host health. The large intestine harbors a complex microbial ecosystem comprising several hundreds of different bacterial species, which complicates investigations on intestinal physiology and ecology. To facilitate such studies, a highly simplified microbiota consisting of 14 anaerobic and facultatively anaerobic organisms (Bacteroi

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Do Thi, Men, Le Pham Thi, Tuan Tran Van, Tuyet Lan Ninh Thị, and Minh Huyen Nguyen Thi. "Isolation and identification of Bifidobacterium spp. from infant intestinal tract." Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam 3, no. 2 (June 30, 2020): 125–32. http://dx.doi.org/10.47866/2615-9252/vjfc.104.

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As one of probiotic bacteria, Bifidobacterium is supplement in food industry such as in milk and yogurt. The advantageous impact of these bacteria for human health was reported. However, in Vietnam, supplement of these bacteria as probiotic source to the food was not much in food industry. In our experiment, Bifidobacterium are isolated from a feces sample of 7 days-child which only had mother milk. The bacterial isolated from infant intestinal tract was Bifidobacterium infantis (B. infantis, closest to Bifidobacterium longum ssp. infantis strain Bi-26 with gene bank accession CP054425.1). The

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Ben-Amor, Kaouther, Hans Heilig, Hauke Smidt, Elaine E. Vaughan, Tjakko Abee, and Willem M. de Vos. "Genetic Diversity of Viable, Injured, and Dead Fecal Bacteria Assessed by Fluorescence-Activated Cell Sorting and 16S rRNA Gene Analysis." Applied and Environmental Microbiology 71, no. 8 (August 2005): 4679–89. http://dx.doi.org/10.1128/aem.71.8.4679-4689.2005.

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ABSTRACT A novel approach combining a flow cytometric in situ viability assay with 16S rRNA gene analysis was used to study the relationship between diversity and activity of the fecal microbiota. Simultaneous staining with propidium iodide (PI) and SYTO BC provided clear discrimination between intact cells (49%), injured or damaged cells (19%), and dead cells (32%). The three subpopulations were sorted and characterized by denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene amplicons obtained from the total and bifidobacterial communities. This analysis revealed that not only the

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Brunner-Weinzierl, Monika C., Mandy Pierau, Dirk Schlueter, Karin Lampe, Christoph Arens, Gerhard Jorch, and Vogel Katrin. "CD4+ T cells from human neonates and infants respond variously against antigens of Staphylococcus aureus, but not against Bifidobacter longum ssp. infantis." Journal of Immunology 196, no. 1_Supplement (May 1, 2016): 66.24. http://dx.doi.org/10.4049/jimmunol.196.supp.66.24.

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Abstract T cells in human neonates, infants, and adults differ dramatically in the initiation, strength, and stability of their responses. In this study, we investigate cellular mechanisms of CD4+ T cells from neonates, infants and adults to show the antigen specific response to Staphylococcus aureus and Bifidobacterium longum ssp. infantis. T cells from surgically excised adenoids of infants in different ages, cord blood and peripheral blood from healthy donors were characterized by flow cytometry and functional assays. Therefore CD14+ monocytes were incubated with extracts of Staphylococcus

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Elden Babekir, Wala Salah, Abubakar Awad Siddig, and Barka Mohammed Kabeir. "The Survival of Bifidobacterium infantis 20088 and Physicochemical Changes During Refrigeration Storage of Selected Fermented Traditional Sudanese Fruit Beverages." Turkish Journal of Agriculture - Food Science and Technology 3, no. 11 (October 23, 2015): 866. http://dx.doi.org/10.24925/turjaf.v3i11.866-868.437.

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This study was carried out to evaluate survival of Bifidobacterium infantis 20088 in fermented beverage formulated from selected traditional Sudanese fruits Gudaim and dom. The viable counts of the strain and physiochemical changes during refrigeration were determined. 10% beverages (w/v) were prepared from dom and gudaim powder. 2.5% (w/w) skim milk was supplemented to each formulation to provide the required nutrient for bacteria growth during the fermentation. After sterilization and cooling, the mixture was inoculated with a 10% culture of B. infants 20088 followed by incubation for 36 h a

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Matsuki, Takahiro, Koichi Watanabe, Ryuichiro Tanaka, Masafumi Fukuda, and Hiroshi Oyaizu. "Distribution of Bifidobacterial Species in Human Intestinal Microflora Examined with 16S rRNA-Gene-Targeted Species-Specific Primers." Applied and Environmental Microbiology 65, no. 10 (October 1, 1999): 4506–12. http://dx.doi.org/10.1128/aem.65.10.4506-4512.1999.

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ABSTRACT In order to clarify the distribution of bifidobacterial species in the human intestinal tract, a 16S rRNA-gene-targeted species-specific PCR technique was developed and used with DNAs extracted from fecal samples obtained from 48 healthy adults and 27 breast-fed infants. To cover all of the bifidobacterial species that have been isolated from and identified in the human intestinal tract, species-specific primers for Bifidobacterium longum, B. infantis,B. dentium, and B. gallicum were developed and used with primers for B. adolescentis, B. angulatum, B. bifidum, B. breve, and the B. ca

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Ferro, Lynn, Kameron Sugino, Vanja Klepac-Ceraj, and Sarah Comstock. "Human Milk Oligosaccharide (HMO) Metabolizing Gene Abundances in Human Infant Fecal Samples." Current Developments in Nutrition 5, Supplement_2 (June 2021): 741. http://dx.doi.org/10.1093/cdn/nzab046_038.

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Abstract Objectives To benefit from the complex oligosaccharides found in human milk, an infant relies on microbial metabolism of these compounds. The main objective of this study was to determine the abundance of Bifidobacterium infantis human milk oligosaccharide (HMO) metabolizing genes in infant fecal samples. Methods Fecal samples from 40 6-mos-old infants were collected. Genomic DNA was extracted, and quantitative real-time PCR was used to determine the abundance of several genes in B. infantis. B infantis specific primer sets were used to target 5 HMO metabolizing genes as well as the 1

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Lindner, Cordula, Ellen Looijesteijn, Helmie van Dijck, Ingeborg Bovee-Oudenhoven, Margreet Heerikhuisen, Tim J. van den Broek, Massimo Marzorati, Vassilis Triantis, and Arjen Nauta. "Infant Fecal Fermentations with Galacto-Oligosaccharides and 2′-Fucosyllactose Show Differential Bifidobacterium longum Stimulation at Subspecies Level." Children 10, no. 3 (February 23, 2023): 430. http://dx.doi.org/10.3390/children10030430.

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The objective of the current study was to evaluate the potential of 2′-FL and GOS, individually and combined, in beneficially modulating the microbial composition of infant and toddler (12–18 months) feces using the micro-Matrix bioreactor. In addition, the impacts of GOS and 2′-FL, individually and combined, on the outgrowth of fecal bifidobacteria at (sub)species level was investigated using the baby M-SHIME® model. For young toddlers, significant increases in the genera Bifidobacterium, Veillonella, and Streptococcus, and decreases in Enterobacteriaceae, Clostridium XIVa, and Roseburia were

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LoCascio, Riccardo G., Prerak Desai, David A. Sela, Bart Weimer, and David A. Mills. "Broad Conservation of Milk Utilization Genes in Bifidobacterium longum subsp. infantis as Revealed by Comparative Genomic Hybridization." Applied and Environmental Microbiology 76, no. 22 (August 27, 2010): 7373–81. http://dx.doi.org/10.1128/aem.00675-10.

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ABSTRACT Human milk oligosaccharides (HMOs) are the third-largest solid component of milk. Their structural complexity renders them nondigestible to the host but liable to hydrolytic enzymes of the infant colonic microbiota. Bifidobacteria and, frequently, Bifidobacterium longum strains predominate the colonic microbiota of exclusively breast-fed infants. Among the three recognized subspecies of B. longum, B. longum subsp. infantis achieves high levels of cell growth on HMOs and is associated with early colonization of the infant gut. The B. longum subsp. infantis ATCC 15697 genome features fi

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Jena, Rajashree, Prasanta Kumar Choudhury, Anil Kumar Puniya, and Sudhir Kumar Tomar. "Applicability of rpoB Gene for PCR-RFLP based Discrimination of Bifidobacterial Species Isolated from Human and Animal Sources." Journal of Pure and Applied Microbiology 16, no. 1 (February 21, 2022): 503–13. http://dx.doi.org/10.22207/jpam.16.1.48.

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Bifidobacteria are widely used as probiotics for their application in the development of functional food and prophylactic therapy. This has necessitated the development of a molecular approach for the genera to be widely identified up to species and subspecies level. In the current study, PCR-RFLP of the partial RNA polymerase β-subunit (rpoB) gene fragment was evaluated for differential identification of Bifidobacterium species. The rpoB gene partial sequences of 575 bp were amplified from 93 previously identified isolates collected from various sources of human and animal origin along with 1

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Quinn, Erinn M., Helen Slattery, Dan Walsh, Lokesh Joshi, and Rita M. Hickey. "Bifidobacterium longum subsp. infantis ATCC 15697 and Goat Milk Oligosaccharides Show Synergism In Vitro as Anti-Infectives against Campylobacter jejuni." Foods 9, no. 3 (March 17, 2020): 348. http://dx.doi.org/10.3390/foods9030348.

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Bifidobacteria are known to inhibit, compete with and displace the adhesion of pathogens to human intestinal cells. Previously, we demonstrated that goat milk oligosaccharides (GMO) increased the attachment of Bifidobacterium longum subsp. infantis ATCC 15697 to intestinal cells in vitro. In this study, we aimed to exploit this effect as a mechanism for inhibiting pathogen association with intestinal cells. We examined the synergistic effect of GMO-treated B. infantis on preventing the attachment of a highly invasive strain of Campylobacter jejuni to intestinal HT-29 cells. The combination dec

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Manzano, S., J. De Andrés, I. Castro, J. M. Rodríguez, E. Jiménez, and I. Espinosa-Martos. "Safety and tolerance of three probiotic strains in healthy infants: a multi-centre randomized, double-blind, placebo-controlled trial." Beneficial Microbes 8, no. 4 (August 24, 2017): 569–78. http://dx.doi.org/10.3920/bm2017.0009.

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Some strains of species belonging to the genera Bifidobacterium and Lactobacillus are used in order to maintain health. Although these organisms have a long record of safe use, it is important to assess their safety and tolerance in potentially vulnerable populations, such as infants. The objective of this study was to evaluate the safety and tolerance of three probiotic strains (Bifidobacterium longum subsp. infantis R0033, Bifidobacterium bifidum R0071 and Lactobacillus helveticus R0052) in healthy infants aged 3 to 12 months. A multi-centre randomized, double-blind, placebo-controlled inter

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Lueschow, Shiloh R., Timothy J. Boly, Steven A. Frese, Giorgio Casaburi, Ryan D. Mitchell, Bethany M. Henrick, and Steven J. McElroy. "Bifidobacterium longum Subspecies infantis Strain EVC001 Decreases Neonatal Murine Necrotizing Enterocolitis." Nutrients 14, no. 3 (January 24, 2022): 495. http://dx.doi.org/10.3390/nu14030495.

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Necrotizing enterocolitis (NEC) is a disease mainly of preterm infants with a 30–50% mortality rate and long-term morbidities for survivors. Treatment strategies are limited and have not improved in decades, prompting research into prevention strategies, particularly with probiotics. Recent work with the probiotic B. infantis EVC001 suggests that this organism may generate a more appropriate microbiome for preterm infants who generally have inappropriate gut colonization and inflammation, both risk factors for NEC. Experimental NEC involving Paneth cell disruption in combination with bacterial

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Ward, Robert E., Milady Niï¿½onuevo, David A. Mills, Carlito B. Lebrilla, and J. Bruce German. "In Vitro Fermentation of Breast Milk Oligosaccharides by Bifidobacterium infantis and Lactobacillus gasseri." Applied and Environmental Microbiology 72, no. 6 (June 2006): 4497–99. http://dx.doi.org/10.1128/aem.02515-05.

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ABSTRACT It has been proposed that human milk oligosaccharides (HMO) function as a prebiotic for bifidobacteria, yet this activity has not been adequately investigated. In this study, Bifidobacterium infantis was shown to ferment purified HMO as a sole carbon source, while another gut commensal, Lactobacillus gasseri, did not ferment HMO. Our results support the hypothesis that HMO selectively amplify bacterial populations in the infant intestine.

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Nogacka, Alicja M., Isabel Cuesta, Miguel Gueimonde, and Clara G. de los Reyes-Gavilán. "2-Fucosyllactose Metabolism by Bifidobacteria Promotes Lactobacilli Growth in Co-Culture." Microorganisms 11, no. 11 (October 29, 2023): 2659. http://dx.doi.org/10.3390/microorganisms11112659.

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Breastfeeding is recognized as the gold standard in infant nutrition, not only because of breastmilk’s intrinsic nutritional benefits but also due to the high content of different bioactive components such as 2-fucosyllactose (2′FL) in the mother’s milk. It promotes the growth of its two major consumers, Bifidobacterium longum ssp. infantis and Bifidobacterium bifidum, but the effect on other intestinal microorganisms of infant microbiota remains incompletely understood. pH-uncontrolled fecal cultures from infants donors identified as “fast 2′FL -degrader” microbiota phenotype were used for th

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Lamendella, Regina, Jorge W. Santo Domingo, Catherine Kelty, and Daniel B. Oerther. "Bifidobacteria in Feces and Environmental Waters." Applied and Environmental Microbiology 74, no. 3 (November 9, 2007): 575–84. http://dx.doi.org/10.1128/aem.01221-07.

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ABSTRACT Bifidobacteria have been recommended as potential indicators of human fecal pollution in surface waters even though very little is known about their presence in nonhuman fecal sources. The objective of this research was to shed light on the occurrence and molecular diversity of this fecal indicator group in different animals and environmental waters. Genus- and species-specific 16S rRNA gene PCR assays were used to study the presence of bifidobacteria among 269 fecal DNA extracts from 32 different animals. Twelve samples from three wastewater treatment plants and 34 water samples from

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Albarracin, L., R. Komatsu, V. Garcia-Castillo, H. Aso, N. Iwabuchi, J. Z. Xiao, F. Abe, H. Takahashi, J. Villena, and H. Kitazawa. "Deciphering the influence of paraimmunobiotic bifidobacteria on the innate antiviral immune response of bovine intestinal epitheliocytes by transcriptomic analysis." Beneficial Microbes 10, no. 2 (March 13, 2019): 199–209. http://dx.doi.org/10.3920/bm2018.0024.

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Previously, we reported that the non-viable immunomodulatory Bifidobacterium infantis MCC12 and Bifidobacterium breve MCC1274 strains (paraimmunobiotic bifidobacteria) were able to increase the protection against rotavirus infection in bovine intestinal epithelial (BIE) cells. In order to gain insight into the influence of paraimmunobiotic bifidobacteria on the innate antiviral immune response of BIE cells, their effect on the transcriptomic response triggered by Toll-like receptor 3 (TLR3) activation was investigated. By using microarray technology and qPCR analysis, we obtained a global over

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Melsaether, Cathrine, Diana Høtoft, Anja Wellejus, Gerben D. A. Hermes, and Anders Damholt. "Seeding the Infant Gut in Early Life—Effects of Maternal and Infant Seeding with Probiotics on Strain Transfer, Microbiota, and Gastrointestinal Symptoms in Healthy Breastfed Infants." Nutrients 15, no. 18 (September 15, 2023): 4000. http://dx.doi.org/10.3390/nu15184000.

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We investigated the effects of two dosing regimens of two multi-strain probiotic products on the gut microbiota of breastfed infants, including the transfer of the dosed strains and clinical outcomes. In forty-seven dyads, infants were either exposed through maternal intake (MS) of Lactobacillus acidophilus LA-5, Bifidobacterium animalis subsp. lactis BB-12, Lacticaseibacillus rhamnosus LGG, and Bifidobacterium longum subsp. infantis Bifin02 from gestational week thirty-three until four weeks after birth (n = 24) or dosed directly (IS) with the same strains except for LA-5 starting within 24 h

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Sakanaka, Mikiyasu, Morten Ejby Hansen, Aina Gotoh, Toshihiko Katoh, Keisuke Yoshida, Toshitaka Odamaki, Hiroyuki Yachi, et al. "Evolutionary adaptation in fucosyllactose uptake systems supports bifidobacteria-infant symbiosis." Science Advances 5, no. 8 (August 2019): eaaw7696. http://dx.doi.org/10.1126/sciadv.aaw7696.

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The human gut microbiota established during infancy has persistent effects on health. In vitro studies have suggested that human milk oligosaccharides (HMOs) in breast milk promote the formation of a bifidobacteria-rich microbiota in infant guts; however, the underlying molecular mechanism remains elusive. Here, we characterized two functionally distinct but overlapping fucosyllactose transporters (FL transporter-1 and -2) from Bifidobacterium longum subspecies infantis. Fecal DNA and HMO consumption analyses, combined with deposited metagenome data mining, revealed that FL transporter-2 is pr

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Ding, Mengfan, Haiqin Chen, Renqiang Yu, Reynolds Paul Ross, Catherine Stanton, Hao Zhang, Bo Yang, and Wei Chen. "Shared and Non-Shared sIgA-Coated and -Uncoated Bacteria in Intestine of Mother–Infant Pairs." International Journal of Molecular Sciences 23, no. 17 (August 30, 2022): 9873. http://dx.doi.org/10.3390/ijms23179873.

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The infant gut microbiota is critical for promoting and maintaining early-life health. The study aimed to analyze the composition of sIgA-coated and sIgA-uncoated bacterial communities at genus level and lactobacilli and bifidobacterial communities at species level in human breast milk (HBM) and infant and maternal feces. Eleven pregnant women were recruited successfully. HBM; infant feces during colostrum, transition, and mature stages; and maternal feces within the mature stage were collected. sIgA-coated and sIgA-uncoated bacteria were separated with magnetic-activated cell sorting. Then, 1

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Musilova, S., N. Modrackova, P. Hermanova, T. Hudcovic, R. Svejstil, V. Rada, V. Tejnecky, and V. Bunesova. "Assessment of the synbiotic properites of human milk oligosaccharides and Bifidobacterium longum subsp. infantis in vitro and in humanised mice." Beneficial Microbes 8, no. 2 (April 26, 2017): 281–89. http://dx.doi.org/10.3920/bm2016.0138.

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The mode of delivery plays a crucial role in infant gastrointestinal tract colonisation, which in the case of caesarean section is characterised by the presence of clostridia and low bifidobacterial counts. Gut colonisation can be modified by probiotics, prebiotics or synbiotics. Human milk oligosaccharides (HMOs) are infant prebiotics that show a bifidogenic effect. Moreover, genome sequencing of Bifidobacterium longum subsp. infantis within the infant microbiome revealed adaptations for milk utilisation. This study aimed to evaluate the synbiotic effect of B. longum subsp. infantis, HMOs and

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Fortmann, Ingmar, Janina Marißen, Bastian Siller, Juliane Spiegler, Alexander Humberg, Kathrin Hanke, Kirstin Faust, et al. "Lactobacillus Acidophilus/Bifidobacterium Infantis Probiotics Are Beneficial to Extremely Low Gestational Age Infants Fed Human Milk." Nutrients 12, no. 3 (March 22, 2020): 850. http://dx.doi.org/10.3390/nu12030850.

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Objective: To evaluate the nutrition-related effects of prophylactic Lactobacillus acidophilus/Bifidobacterium infantis probiotics on the outcomes of preterm infants <29 weeks of gestation that receive human milk and/or formula nutrition. We hypothesize that human-milk-fed infants benefit from probiotics in terms of sepsis prevention and growth. Methods: We performed an observational study of the German Neonatal Network (GNN) over a period of six years, between 1 January, 2013 and 31 December, 2018. Prophylactic probiotic use of L. acidophilus/B. infantis was evaluated in preterm infants &l

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Đỗ Thị, Mến, Tuyết Lan Ninh Thị, Lệ Phạm Thị, Tuấn Trần Văn та Minh Huyền Nguyễn Thị. "Phân lập và định danh Bifidobacterium spp. từ đường tiêu hóa của trẻ sơ sinh". Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam 3, № 2 (30 червня 2020): 125–32. http://dx.doi.org/10.47866/2615-9252/vjfc.654.

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Là một trong những vi khuẩn probiotic, Bifidobacterium được sử dung để bổ sung vào thực phẩm trong công nghiệp thực phẩm như sữa và sữa chua. Những ảnh hưởng tốt của các vi khuẩn này đến sức khỏe con người đã được công bố. Tuy nhiên, ở Việt Nam, việc bổ sung những vi khuẩn này làm nguồn probiotic trong thực phẩm chưa được chú trọng trong ngành công nghiệp thực phẩm. Trong thí nghiệm này, Bifidobacterium được phân lập từ phân của trẻ bảy ngày tuổi chỉ bú sữa mẹ. Vi khuẩn phân lập được từ đường ruột của một trẻ 7 ngày tuổi tại Việt Nam là Bifidobacterium infantis (B. Infantis gần nhất là Bifidob

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Zhang, Gongsheng, Hui Sun, Zihe Xu, Ze Tan, Lihong Xiao, Mingxue He, Jiaqi Shang, Anna N. Tsapieva, and Lili Zhang. "Screening of Bifidobacteria with Probiotic Potential from Healthy Infant Feces by Using 2′-Fucosyllactose." Foods 12, no. 4 (February 17, 2023): 858. http://dx.doi.org/10.3390/foods12040858.

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Using 2′-fucosyllactose (2′-FL) as the sole carbon source can be an efficient way to screen bifidobacteria with superior probiotic capabilities since 2′-FL is a key element in promoting the growth of intestinal bifidobacteria in newborns. This approach was used in this work to screen eight bifidobacteria strains, including one strain of Bifidobacterium longum subsp. infantis BI_Y46 and seven strains of Bifidobacterium bifidum (BB_Y10, BB_Y30, BB_Y39, BB_S40, BB_H4, BB_H5 and BB_H22). Studies on their probiotic properties showed that BI_Y46 had a unique morphology with pilus-like structure, a h

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