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Artykuły w czasopismach na temat „Bifidobacterium”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 25 lipca 2025

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1

Turroni, Francesca, Elena Foroni, Paola Pizzetti, et al. "Exploring the Diversity of the Bifidobacterial Population in the Human Intestinal Tract." Applied and Environmental Microbiology 75, no. 6 (2009): 1534–45. http://dx.doi.org/10.1128/aem.02216-08.

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ABSTRACT Although the health-promoting roles of bifidobacteria are widely accepted, the diversity of bifidobacteria among the human intestinal microbiota is still poorly understood. We performed a census of bifidobacterial populations from human intestinal mucosal and fecal samples by plating them on selective medium, coupled with molecular analysis of selected rRNA gene sequences (16S rRNA gene and internally transcribed spacer [ITS] 16S-23S spacer sequences) of isolated colonies. A total of 900 isolates were collected, of which 704 were shown to belong to bifidobacteria. Analyses showed that

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2

Dinoto, Achmad, Tatiana M. Marques, Kanta Sakamoto, et al. "Population Dynamics of Bifidobacterium Species in Human Feces during Raffinose Administration Monitored by Fluorescence In Situ Hybridization-Flow Cytometry." Applied and Environmental Microbiology 72, no. 12 (2006): 7739–47. http://dx.doi.org/10.1128/aem.01777-06.

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ABSTRACT The population dynamics of bifidobacteria in human feces during raffinose administration were investigated at the species level by using fluorescence in situ hybridization (FISH) coupled with flow cytometry (FCM) analysis. Although double-staining FISH-FCM using both fluorescein isothiocyanate (FITC) and indodicarbocyanine (Cy5) as labeling dyes for fecal samples has been reported, the analysis was interfered with by strong autofluorescence at the FITC fluorescence region because of the presence of autofluorescence particles/debris in the fecal samples. We circumvented this problem by

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3

Requena, Teresa, Jeremy Burton, Takahiro Matsuki, et al. "Identification, Detection, and Enumeration of Human Bifidobacterium Species by PCR Targeting the Transaldolase Gene." Applied and Environmental Microbiology 68, no. 5 (2002): 2420–27. http://dx.doi.org/10.1128/aem.68.5.2420-2427.2002.

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ABSTRACT Methods that enabled the identification, detection, and enumeration of Bifidobacterium species by PCR targeting the transaldolase gene were tested. Bifidobacterial species isolated from the feces of human adults and babies were identified by PCR amplification of a 301-bp transaldolase gene sequence and comparison of the relative migrations of the DNA fragments in denaturing gradient gel electrophoresis (DGGE). Two subtypes of Bifidobacterium longum, five subtypes of Bifidobacterium adolescentis, and two subtypes of Bifidobacterium pseudocatenulatum could be differentiated using PCR-DG

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4

Sakurai, Takuma, Toshitaka Odamaki, and Jin-zhong Xiao. "Production of Indole-3-Lactic Acid by Bifidobacterium Strains Isolated fromHuman Infants." Microorganisms 7, no. 9 (2019): 340. http://dx.doi.org/10.3390/microorganisms7090340.

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Recent studies have shown that metabolites produced by microbes can be considered as mediators of host-microbial interactions. In this study, we examined the production of tryptophan metabolites by Bifidobacterium strains found in the gastrointestinal tracts of humans and other animals. Indole-3-lactic acid (ILA) was the only tryptophan metabolite produced in bifidobacteria culture supernatants. No others, including indole-3-propionic acid, indole-3-acetic acid, and indole-3-aldehyde, were produced. Strains of bifidobacterial species commonly isolated from the intestines of human infants, such

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5

Mikkelsen, Lene Lind, Christian Bendixen, Mogens Jakobsen, and Bent Borg Jensen. "Enumeration of Bifidobacteria in Gastrointestinal Samples from Piglets." Applied and Environmental Microbiology 69, no. 1 (2003): 654–58. http://dx.doi.org/10.1128/aem.69.1.654-658.2003.

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ABSTRACT The population of Bifidobacterium spp. in fecal samples from suckling piglets was investigated, and Beerens, raffinose-bifidobacterium (RB), and modified Wilkins-Chalgren (MW) agar media were evaluated with regard to the enumeration of bifidobacteria in porcine intestinal samples. The results demonstrated that the population of bifidobacteria in the feces of suckling piglets is numerically low, and a phylogenetic analysis of the 16S rRNA gene from bifidobacterial isolates suggested that a possibly new Bifidobacterium species was isolated. Beerens, RB, and MW agar media were not select

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6

Matsuki, Takahiro, Koichi Watanabe, Junji Fujimoto, et al. "Quantitative PCR with 16S rRNA-Gene-Targeted Species-Specific Primers for Analysis of Human Intestinal Bifidobacteria." Applied and Environmental Microbiology 70, no. 1 (2004): 167–73. http://dx.doi.org/10.1128/aem.70.1.167-173.2004.

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ABSTRACT A highly sensitive quantitative PCR detection method has been developed and applied to the distribution analysis of human intestinal bifidobacteria by combining real-time PCR with Bifidobacterium genus- and species-specific primers. Real-time PCR detection of serially diluted DNA extracted from cultured bifidobacteria was linear for cell counts ranging from 106 to 10 cells per PCR assay. It was also found that the method was applicable to the detection of Bifidobacterium in feces when it was present at concentrations of >106 cells per g of feces. Concerning the distribution of Bifi

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7

Fushinobu, Shinya, and Maher Abou Hachem. "Structure and evolution of the bifidobacterial carbohydrate metabolism proteins and enzymes." Biochemical Society Transactions 49, no. 2 (2021): 563–78. http://dx.doi.org/10.1042/bst20200163.

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Bifidobacteria have attracted significant attention because they provide health-promoting effects in the human gut. In this review, we present a current overview of the three-dimensional structures of bifidobacterial proteins involved in carbohydrate uptake, degradation, and metabolism. As predominant early colonizers of the infant's gut, distinct bifidobacterial species are equipped with a panel of transporters and enzymes specific for human milk oligosaccharides (HMOs). Interestingly, Bifidobacterium bifidum and Bifidobacterium longum possess lacto-N-biosidases with unrelated structural fold

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8

Saturio, Silvia, Alicja M. Nogacka, Guadalupe M. Alvarado-Jasso, et al. "Role of Bifidobacteria on Infant Health." Microorganisms 9, no. 12 (2021): 2415. http://dx.doi.org/10.3390/microorganisms9122415.

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Bifidobacteria are among the predominant microorganisms during infancy, being a dominant microbial group in the healthy breastfed infant and playing a crucial role in newborns and infant development. Not only the levels of the Bifidobacterium genus but also the profile and quantity of the different bifidobacterial species have been demonstrated to be of relevance to infant health. Although no definitive proof is available on the causal association, reduced levels of bifidobacteria are perhaps the most frequently observed alteration of the intestinal microbiota in infant diseases. Moreover, Bif

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9

Peirotén, A., J. L. Arqués, M. Medina, and E. Rodríguez-Mínguez. "Bifidobacterial strains shared by mother and child as source of probiotics." Beneficial Microbes 9, no. 2 (2018): 231–38. http://dx.doi.org/10.3920/bm2017.0133.

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Importance of bifidobacteria as part of the infant intestinal microbiota has been highlighted. Their acquisition is influenced by the mode of birth and the feed regime afterwards, with a special role of the maternal microbiota. The presence of the same shared bifidobacterial strains between breast milk and infant faeces in 14 mother-infant pairs was assessed by means of pulsed-field gel electrophoresis (PFGE) genotyping. Four shared strains of Bifidobacterium breve (2), Bifidobacterium longum subsp. infantis and B. longum subsp. longum were found in breast milk-infant faeces pairs. Two years l

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10

ROY, DENIS, та PIERRE WARD. "Rapid Detection of Bifidobacterium dentium by Enzymatic Hydrolysis of β-Glucuronide Substrates". Journal of Food Protection 55, № 4 (1992): 291–95. http://dx.doi.org/10.4315/0362-028x-55.4.291.

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Enzyme profiles and fermentation patterns of bifidobacteria were studied to determine phenotypic characteristics that allow the rapid detection of Bifidobacterium dentium and its differentiation from Bifidobacterium adolescentis, Bifidobacterium angulatum, Bifidobacterium catenulatum, and Bifidobacterium pseudocatenulatum. Among 43 bifidobacterial strains tested, the production of β-glucuronidase was limited to six strains of B. dentium. The presence of B. dentium on a selective medium may be rapidly confirmed by the detection of β-glucuronidase activity. Columbia agar containing propionic aci

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11

Sirilun, S., H. Takahashi, S. Boonyaritichaikij, et al. "Impact of maternal bifidobacteria and the mode of delivery on Bifidobacterium microbiota in infants." Beneficial Microbes 6, no. 6 (2015): 767–74. http://dx.doi.org/10.3920/bm2014.0124.

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The aim of this study is to examine the influence of maternal intestinal and vaginal bifidobacteria on the colonisation of bifidobacteria in the gut of infants. Faecal samples from 120 healthy pregnant mothers within 1 month of delivery and from their infants at 1 month of age and 98 vaginal swabs from the mothers at the time of delivery were collected at a maternity hospital in Chiang Mai, Thailand. The faecal and vaginal samples were assayed by real-time PCR assays to detect Bifidobacterium species and to estimate the bifidobacterial copy numbers. After adjusting for the numbers of each Bifi

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12

Chen, Yuan Yao, Xin Zhao, Wolfgang Moeder, et al. "Impact of Maternal Intrapartum Antibiotics, and Caesarean Section with and without Labour on Bifidobacterium and Other Infant Gut Microbiota." Microorganisms 9, no. 9 (2021): 1847. http://dx.doi.org/10.3390/microorganisms9091847.

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Background and Aims: Few studies consider the joint effect of multiple factors related to birth, delivery mode, intrapartum antibiotic prophylaxis and the onset of labour, on the abundance of Bifidobacterium and the quantity of this genus and its species Bifidobacterium longum subsp. infantis in the infant gut microbiota. We implemented such a study. Methods: Among 1654 Canadian full-term infants, the gut microbiota of faecal samples collected at 3 months were profiled by 16S rRNA sequencing; the genus Bifidobacterium and Bifidobacterium longum subsp. infantis were quantified by qPCR. Associat

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13

Killer, J., J. Kopečný, J. Mrázek, et al. "Bifidobacterium actinocoloniiforme sp. nov. and Bifidobacterium bohemicum sp. nov., from the bumblebee digestive tract." International Journal of Systematic and Evolutionary Microbiology 61, no. 6 (2011): 1315–21. http://dx.doi.org/10.1099/ijs.0.022525-0.

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Our previous study, based primarily on PCR-denaturing gradient gel electrophoresis and 16S rRNA gene sequencing, focused on the isolation of four bifidobacterial groups from the digestive tract of three bumblebee species. In that study, we proposed that these isolated groups potentially represented novel species of the family Bifidobacteriaceae. One of the four, Bifidobacterium bombi, has been described recently. Strains representing two of the other groups have been classified as members of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase act

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14

Young, Sarah L., Mary A. Simon, Margaret A. Baird, et al. "Bifidobacterial Species Differentially Affect Expression of Cell Surface Markers and Cytokines of Dendritic Cells Harvested from Cord Blood." Clinical Diagnostic Laboratory Immunology 11, no. 4 (2004): 686–90. http://dx.doi.org/10.1128/cdli.11.4.686-690.2004.

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ABSTRACT The gut microbiota may be important in the postnatal development of the immune system and hence may influence the prevalence of atopic diseases. Bifidobacteria are the most numerous bacteria in the guts of infants, and the presence or absence of certain species could be important in determining the geographic incidence of atopic diseases. We compared the fecal populations of bifidobacteria from children aged 25 to 35 days in Ghana (which has a low prevalence of atopy), New Zealand, and the United Kingdom (high-prevalence countries). Natal origin influenced the detection of bifidobacte

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15

Bottacini, Francesca, Duccio Medini, Angelo Pavesi, et al. "Comparative genomics of the genus Bifidobacterium." Microbiology 156, no. 11 (2010): 3243–54. http://dx.doi.org/10.1099/mic.0.039545-0.

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Whole-genome sequencing efforts have revolutionized the study of bifidobacterial genetics and physiology. Unfortunately, the sequence of a single genome does not provide information on bifidobacterial genetic diversity and on how genetic variability supports improved adaptation of these bacteria to the environment of the human gastrointestinal tract (GIT). Analysis of nine genomes from bifidobacterial species showed that such genomes display an open pan-genome structure. Mathematical extrapolation of the data indicates that the genome reservoir available to the bifidobacterial pan-genome consi

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16

Chaplin, A. V., A. G. Brzhozovskii, T. V. Parfenova, et al. "Species Diversity of Bifidobacteria in the Intestinal Microbiota Studied Using MALDI-TOF Mass-Spectrometry." Annals of the Russian academy of medical sciences 70, no. 4 (2015): 435–40. http://dx.doi.org/10.15690/vramn.v70.i4.1409.

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Background: The members of genus Bifidobacterium represent a significant part of intestinal microbiota in adults and predominate in infants. Species repertoire of the intestinal bifidobacteria is known to be subjected to major changes with age; however, many details of this process are still to be elucidated.Objective: Our aim was to study the diversity of intestinal bifidobacteria and changes of their qualitative and quantitative composition characteristics during the process of growing up using MALDI-TOF mass-spectrometric analysis of pure bacterial cultures.Methods: A cross-sectional study

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17

Mancino, Walter, Gabriele Andrea Lugli, Douwe van Sinderen, Marco Ventura, and Francesca Turroni. "Mobilome and Resistome Reconstruction from Genomes Belonging to Members of the Bifidobacterium Genus." Microorganisms 7, no. 12 (2019): 638. http://dx.doi.org/10.3390/microorganisms7120638.

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Specific members of the genus Bifidobacterium are among the first colonizers of the human/animal gut, where they act as important intestinal commensals associated with host health. As part of the gut microbiota, bifidobacteria may be exposed to antibiotics, used in particular for intrapartum prophylaxis, especially to prevent Streptococcus infections, or in the very early stages of life after the birth. In the current study, we reconstructed the in silico resistome of the Bifidobacterium genus, analyzing a database composed of 625 bifidobacterial genomes, including partial assembled strains wi

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18

Satokari, Reetta M., Elaine E. Vaughan, Antoon D. L. Akkermans, Maria Saarela, and Willem M. de Vos. "Bifidobacterial Diversity in Human Feces Detected by Genus-Specific PCR and Denaturing Gradient Gel Electrophoresis." Applied and Environmental Microbiology 67, no. 2 (2001): 504–13. http://dx.doi.org/10.1128/aem.67.2.504-513.2001.

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ABSTRACT We describe the development and validation of a method for the qualitative analysis of complex bifidobacterial communities based on PCR and denaturing gradient gel electrophoresis (DGGE).Bifidobacterium genus-specific primers were used to amplify an approximately 520-bp fragment from the 16S ribosomal DNA (rDNA), and the fragments were separated in a sequence-specific manner in DGGE. PCR products of the same length from different bifidobacterial species showed good separation upon DGGE. DGGE of fecal 16S rDNA amplicons from five adult individuals showed host-specific populations of bi

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Woodmansey, Emma J., Marion E. T. McMurdo, George T. Macfarlane, and Sandra Macfarlane. "Comparison of Compositions and Metabolic Activities of Fecal Microbiotas in Young Adults and in Antibiotic-Treated and Non-Antibiotic-Treated Elderly Subjects." Applied and Environmental Microbiology 70, no. 10 (2004): 6113–22. http://dx.doi.org/10.1128/aem.70.10.6113-6122.2004.

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ABSTRACT The colonic microbiota mediates many cellular and molecular events in the host that are important to health. These processes can be affected in the elderly, because in some individuals, the composition and metabolic activities of the microbiota change with age. Detailed characterizations of the major groups of fecal bacteria in healthy young adults, in healthy elderly people, and in hospitalized elderly patients receiving antibiotics were made in this study, together with measurements of their metabolic activities, by analysis of fecal organic acid and ammonia concentrations. The resu

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Martï¿½n, Rocï¿½o, Esther Jimï¿½nez, Hans Heilig, et al. "Isolation of Bifidobacteria from Breast Milk and Assessment of the Bifidobacterial Population by PCR-Denaturing Gradient Gel Electrophoresis and Quantitative Real-Time PCR." Applied and Environmental Microbiology 75, no. 4 (2008): 965–69. http://dx.doi.org/10.1128/aem.02063-08.

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ABSTRACT The objective of this work was to elucidate if breast milk contains bifidobacteria and whether they can be transmitted to the infant gut through breastfeeding. Twenty-three women and their respective infants provided samples of breast milk and feces, respectively, at days 4 to 7 after birth. Gram-positive and catalase-negative isolates from specific media with typical bifidobacterial shapes were identified to the genus level by F6PPK (fructose-6-phosphate phosphoketolase) assays and to the species level by 16S rRNA gene sequencing. Bifidobacterial communities in breast milk were asses

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Mao, Bingyong, Jiayu Gu, Dongyao Li, et al. "Effects of Different Doses of Fructooligosaccharides (FOS) on the Composition of Mice Fecal Microbiota, Especially the Bifidobacterium Composition." Nutrients 10, no. 8 (2018): 1105. http://dx.doi.org/10.3390/nu10081105.

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Fructooligosaccharides (FOS) are a well-known class of prebiotic and are considered to selectively stimulate the growth of bifidobacteria in the gut. Previous studies focused on the growth stimulation of Bifidobacterium, but they did not further investigate the bifidobacterial composition and the specific species that were stimulated. In this study, mice were fed with FOS in different doses for four weeks and the composition of fecal microbiota, in particular Bifidobacterium, was analyzed by sequencing the V3–V4 region and the groEL gene on the MiSeq platform, respectively. In the high-dose gr

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Ročková, Š., V. Rada, J. Havlík, et al. "Growth of bifidobacteria in mammalian milk." Czech Journal of Animal Science 58, No. 3 (2013): 99–105. http://dx.doi.org/10.17221/6666-cjas.

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Microbial colonization of the mammalian intestine begins at birth, when from a sterile state a newborn infant is exposed to an external environment rich in various bacterial species. An important group of intestinal bacteria comprises bifidobacteria. Bifidobacteria represent major intestinal microbiota during the breast-feeding period. Animal milk contains all crucial nutrients for babies&rsquo; intestinal microflora. The aim of our work was to test the influence of different mammalian milk on the growth of bifidobacteria. The growth of seven strains of bifidobacteria in human milk, the co

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Esaiassen, Eirin, Erik Hjerde, Jorunn Pauline Cavanagh, Gunnar Skov Simonsen, and Claus Klingenberg. "Bifidobacterium Bacteremia: Clinical Characteristics and a Genomic Approach To Assess Pathogenicity." Journal of Clinical Microbiology 55, no. 7 (2017): 2234–48. http://dx.doi.org/10.1128/jcm.00150-17.

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ABSTRACT Bifidobacteria are commensals that colonize the orogastrointestinal tract and rarely cause invasive human infections. However, an increasing number of bifidobacterial blood culture isolates has lately been observed in Norway. In order to investigate the pathogenicity of the Bifidobacterium species responsible for bacteremia, we studied Bifidobacterium isolates from 15 patients for whom cultures of blood obtained from 2013 to 2015 were positive. We collected clinical data and analyzed phenotypic and genotypic antibiotic susceptibility. All isolates (11 Bifidobacterium longum , 2 B. bre

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Makino, H., R. Martin, E. Ishikawa, et al. "Multilocus sequence typing of bifidobacterial strains from infant’s faeces and human milk: are bifidobacteria being sustainably shared during breastfeeding?" Beneficial Microbes 6, no. 4 (2015): 563–72. http://dx.doi.org/10.3920/bm2014.0082.

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Bifidobacteria are considered to be one of the most important beneficial intestinal bacteria for infants, contributing to the priming of the mucosal immune system. These microbes can also be detected in mother’s milk, suggesting a potential role of human milk in the colonisation of infant’s gut. However, little is known about the timing of bacteria appearance in human milk, and whether human milk is the first source of inoculation. Here, we investigated whether specific strains are shared sustainably between maternal milk and infant’s gut. Faecal samples and human milk were collected from 102

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Liu, Shijie, Zhifeng Fang, Hongchao Wang, et al. "Gene–Phenotype Associations Involving Human-Residential Bifidobacteria (HRB) Reveal Significant Species- and Strain-Specificity in Carbohydrate Catabolism." Microorganisms 9, no. 5 (2021): 883. http://dx.doi.org/10.3390/microorganisms9050883.

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Bifidobacteria are among the first colonizers of the human gastrointestinal tract. Different bacterial species use different mechanisms for utilization of various carbon sources in order to establish themselves in the complex microbial ecosystem of the gut. However, these mechanisms still need to be explored. Here, a large gene–phenotype correlation analysis was carried out to explore the metabolic and genetic diversity of bifidobacterial carbohydrate utilization abilities. In this study, we used 21 different carbohydrates to determine the growth phenotypes, the distribution of glycoside hydro

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Moubareck, C., M. Lecso, E. Pinloche, M. J. Butel та F. Doucet-Populaire. "Inhibitory Impact of Bifidobacteria on the Transfer of β-Lactam Resistance among Enterobacteriaceae in the Gnotobiotic Mouse Digestive Tract". Applied and Environmental Microbiology 73, № 3 (2006): 855–60. http://dx.doi.org/10.1128/aem.02001-06.

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ABSTRACT While looking for new means to limit the dissemination of antibiotic resistance, we evaluated the role of potentially probiotic bifidobacteria on the transfer of resistance genes between enterobacteria. Transfers of bla genes encoding extended-spectrum β-lactamases (SHV-5 and CTX-M-15) were studied in the absence or presence of bifidobacteria. In vitro, transfer frequencies of these bla genes decreased significantly in the presence of three of five tested strains, i.e., Bifidobacterium longum CUETM-89-215, Bifidobacterium bifidum CIP-56.7T, and Bifidobacterium pseudocatenulatum CIP-10

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Bozkurt, Hüseyin. "PROBIOTIC BIFIDOBACTERIUM AND A NEW GENERATION POSTBIOTIC: ALGINIC ACID." Inflammatory Bowel Diseases 29, Supplement_1 (2023): S48. http://dx.doi.org/10.1093/ibd/izac247.091.

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Abstract AIMS OF THE STUDY According to International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus (2019),Postbiotics are defined as a preparation of inanimate microorganisms and/or their components that confers a health benefit on the host. Postbiotic Alginic acid, also called algin, is a naturally occurring, edible exopolysaccharide found in brown algae. Alginic acid and its Na/Ca salt form of Alginate show the capability in water-binding, water-retention water, and immense swelling and gelation, which could act as a protective barrier via promoting biofilm formatio

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Gueimonde, Miguel, Christel Garrigues, Douwe van Sinderen, Clara G. de los Reyes-Gavilï¿½n, and Abelardo Margolles. "Bile-Inducible Efflux Transporter from Bifidobacterium longum NCC2705, Conferring Bile Resistance." Applied and Environmental Microbiology 75, no. 10 (2009): 3153–60. http://dx.doi.org/10.1128/aem.00172-09.

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ABSTRACT Bifidobacteria are normal inhabitants of the human gut. Some strains of this genus are considered health promoting or probiotic, being included in numerous food products. In order to exert their health benefits, these bacteria must overcome biological barriers, including bile salts, to colonize and survive in specific parts of the intestinal tract. The role of multidrug resistance (MDR) transporters in bile resistance of probiotic bacteria and the effect of bile on probiotic gene expression are not fully understood. In the present study, the effect of subinhibitory concentrations of b

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Candela, Marco, Simone Bergmann, Manuela Vici, et al. "Binding of Human Plasminogen to Bifidobacterium." Journal of Bacteriology 189, no. 16 (2007): 5929–36. http://dx.doi.org/10.1128/jb.00159-07.

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ABSTRACT Bifidobacteria constitute up to 3% of the total microbiota and represent one of the most important health-promoting bacterial groups of the human intestinal microflora. The presence of Bifidobacterium in the human gastrointestinal tract has been directly related to several health-promoting activities; however, to date, no information about the specific mechanisms of interaction with the host is available. In order to provide some insight into the molecular mechanisms involved in the interaction with the host, we investigated whether Bifidobacterium was able to capture human plasminoge

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Satti, Maria, Monica Modesto, Akihito Endo, Takeshi Kawashima, Paola Mattarelli, and Masanori Arita. "Host-Diet Effect on the Metabolism of Bifidobacterium." Genes 12, no. 4 (2021): 609. http://dx.doi.org/10.3390/genes12040609.

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Bifidobacterium has a diverse host range and shows several beneficial properties to the hosts. Many species should have co-evolved with their hosts, but the phylogeny of Bifidobacterium is dissimilar to that of host animals. The discrepancy could be linked to the niche-specific evolution due to hosts’ dietary carbohydrates. We investigated the relationship between bifidobacteria and their host diet using a comparative genomics approach. Since carbohydrates are the main class of nutrients for bifidobacterial growth, we examined the distribution of carbohydrate-active enzymes, in particular glyc

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Hilliard, Margaret A., and David A. Sela. "Transmission and Persistence of Infant Gut-Associated Bifidobacteria." Microorganisms 12, no. 5 (2024): 879. http://dx.doi.org/10.3390/microorganisms12050879.

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Bifidobacterium infantis are the primary colonizers of the infant gut, yet scientific research addressing the transmission of the genus Bifidobacterium to infants remains incomplete. This review examines microbial reservoirs of infant-type Bifidobacterium that potentially contribute to infant gut colonization. Accordingly, strain inheritance from mother to infant via the fecal-oral route is likely contingent on the bifidobacterial strain and phenotype, whereas transmission via the vaginal microbiota may be restricted to Bifidobacterium breve. Additional reservoirs include breastmilk, horizonta

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Olszewska, M., B. Staniewski, and Ł. Łaniewska-Trokenheim. "Cell viability of Bifidobacterium lactis strain in long-term storage butter assessed with the plate count and fluorescence techniques." Czech Journal of Food Sciences 30, No. 5 (2012): 421–28. http://dx.doi.org/10.17221/330/2011-cjfs.

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Great interest in functional products containing bacterial strains displaying health-promoting properties is expressed worldwide and is as a result connected with a demand for developing new probiotic-based products, especially those containing bifidobacteria. The Bifidobacterium strains play a key role in gastrointestinal homeostasis, providing many health-related attributes, but as fastidious microorganisms require specific conditions (e.g. anaerobic environment, neutral pH) to survive in the long-term at the needed level above 10<sup>6</sup>&nbsp;cfu/g. In consequence, not e

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Ménard, Odile, Marie-José Butel, Valérie Gaboriau-Routhiau, and Anne-Judith Waligora-Dupriet. "Gnotobiotic Mouse Immune Response Induced by Bifidobacterium sp. Strains Isolated from Infants." Applied and Environmental Microbiology 74, no. 3 (2007): 660–66. http://dx.doi.org/10.1128/aem.01261-07.

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ABSTRACT Bifidobacterium, which is a dominant genus in infants’ fecal flora and can be used as a probiotic, has shown beneficial effects in various pathologies, including allergic diseases, but its role in immunity has so far been little known. Numerous studies have shown the crucial role of the initial intestinal colonization in the development of the intestinal immune system, and bifidobacteria could play a major role in this process. For a better understanding of the effect of Bifidobacterium on the immune system, we aimed at determining the impact of Bifidobacterium on the T-helper 1 (TH1)

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Gueimonde, Miguel, Shinji Sakata, Marko Kalliomäki, Erika Isolauri, Yoshimi Benno, and Seppo Salminen. "Effect of Maternal Consumption of Lactobacillus GG on Transfer and Establishment of Fecal Bifidobacterial Microbiota in Neonates." Journal of Pediatric Gastroenterology and Nutrition 42, no. 2 (2006): 166–70. http://dx.doi.org/10.1002/j.1536-4801.2006.tb00014.x.

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ABSTRACTBackground:Establishment of the gut microbiota at birth provides a substantial source of microbial stimuli for the maturation of the immune system. Deviations in this process precede the development of specific diseases providing the rationale for the use of probiotics to counteract them.Objective:This study was designed to characterize both the mother‐infant bifidobacteria transfer at birth and the development of bifidobacteria microbiota during the first weeks of life in infants whose mothers received Lactobacillus rhamnosus GG or placebo.Methods:Species‐specific PCR was used to asse

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Abdelazez, Amro, Zafarullah Muhammad, Qiu-Xue Zhang, et al. "Production of a Functional Frozen Yogurt Fortified with Bifidobacterium spp." BioMed Research International 2017 (2017): 1–10. http://dx.doi.org/10.1155/2017/6438528.

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Frozen dairy products have characteristics of both yogurt and ice cream and could be the persuasive carriers of probiotics. Functions of the frozen yogurt containing viable bifidobacterial cells are recognized and favored by the people of all ages. We developed a kind of yogurt supplemented by Bifidobacterium species. Firstly, five strains of Bifidobacterium spp. (Bifidobacterium bifidum ATCC 11547, Bifidobacterium longum ATCC 11549, Bifidobacterium infantis ATCC 11551, Bifidobacterium adolescentis ATCC 11550, and Bifidobacterium breve ATCC 11548) were evaluated based on the feasibility criter

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Newton, Dorothy F., Sandra Macfarlane, and George T. Macfarlane. "Effects of Antibiotics on Bacterial Species Composition and Metabolic Activities in Chemostats Containing Defined Populations of Human Gut Microorganisms." Antimicrobial Agents and Chemotherapy 57, no. 5 (2013): 2016–25. http://dx.doi.org/10.1128/aac.00079-13.

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ABSTRACTThe composition and metabolic activities of the human colonic microbiota are modulated by a number of external factors, including diet and antibiotic therapy. Changes in the structure and metabolism of the gut microbiota may have long-term consequences for host health. The large intestine harbors a complex microbial ecosystem comprising several hundreds of different bacterial species, which complicates investigations on intestinal physiology and ecology. To facilitate such studies, a highly simplified microbiota consisting of 14 anaerobic and facultatively anaerobic organisms (Bacteroi

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Haarman, Monique, and Jan Knol. "Quantitative Real-Time PCR Assays To Identify and Quantify Fecal Bifidobacterium Species in Infants Receiving a Prebiotic Infant Formula." Applied and Environmental Microbiology 71, no. 5 (2005): 2318–24. http://dx.doi.org/10.1128/aem.71.5.2318-2324.2005.

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ABSTRACT A healthy intestinal microbiota is considered to be important for priming of the infants' mucosal and systemic immunity. Breast-fed infants typically have an intestinal microbiota dominated by different Bifidobacterium species. It has been described that allergic infants have different levels of specific Bifidobacterium species than healthy infants. For the accurate quantification of Bifidobacterium adolescentis, Bifidobacterium angulatum, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium catenulatum, Bifidobacterium dentium, Bifidobacterium infantis, and Bifidobacterium

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Yang, Bo, Yingqi Chen, Catherine Stanton, et al. "Bifidobacterium and Lactobacillus Composition at Species Level and Gut Microbiota Diversity in Infants before 6 Weeks." International Journal of Molecular Sciences 20, no. 13 (2019): 3306. http://dx.doi.org/10.3390/ijms20133306.

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Our objective was to investigate the effects of different delivery and feeding modes on the gut microbiota composition of early infants with special emphasis on Bifidobacterium and Lactobacillus profiles at species level. 16S rRNA V3-V4 regions, bifidobacterial, and lactobacilli groEL genes from infant feces were sequenced by Illumina MiSeq. Gut microbiota abundance was significantly different, where standard vaginally delivered (SVD) and breast-fed (BF) groups were higher in comparison with caesarean section (CS), milk-powder-fed (MPF), and mixed-fed (MF) groups. The genus unclassified Entero

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DELCENSERIE, V., N. BECHOUX, T. LÉONARD, B. CHINA, and G. DAUBE. "Discrimination between Bifidobacterium Species from Human and Animal Origin by PCR–Restriction Fragment Length Polymorphism." Journal of Food Protection 67, no. 6 (2004): 1284–88. http://dx.doi.org/10.4315/0362-028x-67.6.1284.

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Bifidobacteria are normal intestinal flora in humans and animals. The genus Bifidobacterium includes 31 species of significant host specificity. Taking into account their properties, we proposed to use bifidobacteria as fecal contamination indicators. PCR–restriction fragment length polymorphism on the 16S rDNA gene was used to distinguish the different Bifidobacterium species. Sixty-four strains belonging to 13 different species were differentiated from animal or human origin using one or two restriction enzymes. Moreover, the primers used were specifics of the Bifidobacterium genus. Therefor

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Rovik, Anwar, Ahmad Reza Maulana, Silmi Qurrotu Aini, and Dyah Fitri Kushayarti. "In Silico Evaluation Reveals The Potential Risk of Antimicrobial Resistance in Bifidobacterium." Al-Kauniyah: Jurnal Biologi 18, no. 1 (2024): 57–64. http://dx.doi.org/10.15408/kauniyah.v1i1.34873.

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AbstractBifidobacteria have beneficial health effects for their hosts. However, they may acquire antibiotic-resistance genes. They may transfer antibiotic-resistance genes to pathogenic microbes found in the human intestine resulting in the emergence of antibiotic-resistant pathogens. This study aimed to predict their resistance to antibiotics by analyzing the whole genome sequence. The entire genome data of Bifidobacterium spp. were obtained from the National Center for Biotechnology Information (NCBI). This study included five Bifidobacterium strains of human origin, five strains of animal o

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Saturio, Silvia, Alicja M. Nogacka, Marta Suárez, et al. "Early-Life Development of the Bifidobacterial Community in the Infant Gut." International Journal of Molecular Sciences 22, no. 7 (2021): 3382. http://dx.doi.org/10.3390/ijms22073382.

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The establishment of the gut microbiota poses implications for short and long-term health. Bifidobacterium is an important taxon in early life, being one of the most abundant genera in the infant intestinal microbiota and carrying out key functions for maintaining host-homeostasis. Recent metagenomic studies have shown that different factors, such as gestational age, delivery mode, or feeding habits, affect the gut microbiota establishment at high phylogenetic levels. However, their impact on the specific bifidobacterial populations is not yet well understood. Here we studied the impact of the

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Tsuji, H., R. Oozeer, K. Matsuda, et al. "Molecular monitoring of the development of intestinal microbiota in Japanese infants." Beneficial Microbes 3, no. 2 (2012): 113–25. http://dx.doi.org/10.3920/bm2011.0038.

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The faecal microbiota of 166 healthy Japanese newborns was analysed periodically from day 1 after birth until the age of 3 years by using the reverse transcription-quantitative PCR. Faecal pH and the organic acid concentration were also examined. Colonisation by both facultative anaerobes and strict anaerobes was confirmed in 95% of the meconium tested. Bifidobacterium-predominant microbiota was established subsequently in most of the infants by 3 months after birth. Bifidobacterium breve, Bifidobacterium longum, Bifidobacterium infantis, Bifidobacterium catenulatum group and Bifidobacterium b

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Pozo-Rubio, T., J. R. Mujico, A. Marcos, et al. "Immunostimulatory effect of faecal Bifidobacterium species of breast-fed and formula-fed infants in a peripheral blood mononuclear cell/Caco-2 co-culture system." British Journal of Nutrition 106, no. 8 (2011): 1216–23. http://dx.doi.org/10.1017/s0007114511001656.

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Bifidobacterium spp. typical of the human intestinal microbiota are believed to influence the balance of immune responses in the intestinal mucosa. The aim of the present study was to investigate the effect of different bifidobacterial species and their mixtures in in vitro experiments with peripheral blood mononuclear cells (PBMC) and Caco-2 cells. Bifidobacterium adolescentis, B. angulatum, B. breve, B. catenulatum, B. infantis, B. longum and two combinations of these bifidobacteria simulating the species composition found in faecal samples from breast-fed (BF) and formula-fed (FF) infants w

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Watanabe, Ayako, Yoshihiro Kadota, Hijiri Yokoyama, et al. "Experimental Determination of the Threshold Dose for Bifidogenic Activity of Dietary 1-Kestose in Rats." Foods 9, no. 1 (2019): 4. http://dx.doi.org/10.3390/foods9010004.

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1-Kestose is a non-digestible oligosaccharide consisting of glucose linked to two fructose units. While 1-kestose is not digested in the small intestine of mammals, it is fermented in the ceca and colon, where the growth of bifidobacteria is promoted. In the present study, we assessed the threshold dose of dietary 1-kestose that increased cecal bifidobacterial levels in rats. Rats were fed experimental diets containing 0% to 0.3% 1-kestose for four weeks. The levels of the genus Bifidobacterium and total gut bacteria were significantly increased in cecal samples of rats fed the 0.3% 1-kestose

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Duranti, Sabrina, Francesca Turroni, Christian Milani, et al. "Exploration of the Genomic Diversity and Core Genome of the Bifidobacterium adolescentis Phylogenetic Group by Means of a Polyphasic Approach." Applied and Environmental Microbiology 79, no. 1 (2012): 336–46. http://dx.doi.org/10.1128/aem.02467-12.

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ABSTRACT In the current work, we describe genome diversity and core genome sequences among representatives of three bifidobacterial species, i.e., Bifidobacterium adolescentis , Bifidobacterium catenulatum , and Bifidobacterium pseudocatenulatum , by employing a polyphasic approach involving analysis of 16S rRNA gene and 16S-23S internal transcribed spacer (ITS) sequences, pulsed-field gel electrophoresis (PFGE), and comparative genomic hybridization (CGH) assays.

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Khavkin, A. I., O. V. Bukharin, N. B. Perunova, E. V. Ivanova, and S. I. Sitkin. "Biological properties and symbiotic relationships of human bifidobacteria." Voprosy praktičeskoj pediatrii 18, no. 2 (2023): 54–64. http://dx.doi.org/10.20953/1817-7646-2023-2-54-64.

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Bifidobacteria, despite more than a century of research history, attract the attention of researchers. Currently, more than 100 species of the Bifidobacterium genus, belonging to the Actinomycetota phylum, have been described. An analysis of the biological properties of bifidobacteria during the formation of symbiotic relationships with the human host revealed several functional clusters, among which one group of bifidobacteria is involved in the cytokine balance, the second recognizes microsymbionts, protecting the biotope from pathogens, and the third supports the barrier function of enteroc

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Baiguzhina, Zh S., A. E. Hasenova, Sh K. Yeleupayeva, et al. "The importance of bacteria of the genus Bifidobacterium in intestinal microbiocenosis." Bulletin of the Karaganda University “Biology medicine geography Series” 11529, no. 3 (2024): 34–44. http://dx.doi.org/10.31489/2024bmg3/34-44.

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Bifidobacteria are the most common microorganisms in the intestines of healthy breastfed children. The genus Bifidobacterium includes bacteria characterized by probiotic properties, such as the induction of immunomodulators, increasing the nutritional value of products due to the assimilation of substrates that are not broken down by the host, anticarcinogenic activity, synthesis of vitamins, production of antimicrobial drugs, which contribute to the promotion of health. Bifidobacteria demonstrate physiological and genetic characteristics including adhesion to the intestinal epithelium, as wel

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Danylenko, Svitlana, Lolita Marynchenko, Viktoriia Bortnyk, Oksana Potemska, and Olena Nizhelska. "Use of Highly Dispersed Silica in Biotechnology of Complex Probiotic Product Based on Bifidobacteria." Innovative Biosystems and Bioengineering 6, no. 1 (2022): 16–24. http://dx.doi.org/10.20535/ibb.2022.6.1.256179.

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Background. The probiotics immobilization technology is one of the most effective ways for controlled and continuous delivery of viable cells into the intestine. It is well known that multifaceted physiological roles of bifidobacteria are to normalize and stabilize the microbiocenosis, to form intestine colonization resistance, to synthesis amino acids, proteins and vitamins, to maintain non-specific resistance of the organism and so all. Such a wide range of positive effects on the macroorganism allows us to consider bifidobacteria as a basis for functional immobilized healthcare products dev

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Yang, Bo, Shuang Yan, Yang Chen, et al. "Diversity of Gut Microbiota and Bifidobacterial Community of Chinese Subjects of Different Ages and from Different Regions." Microorganisms 8, no. 8 (2020): 1108. http://dx.doi.org/10.3390/microorganisms8081108.

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Gut microbiota composition and functionality are closely linked to host health. In this study, the fecal microbiota and bifidobacterial communities of 111 healthy volunteers from four regions of China of varying age profiles (Child, 1–5 years; Young, 18–50 years; Elder, 60–80 years; Longevity, ≥90 years) were investigated via high-throughput sequencing. Canonical analysis revealed that the gut microbiota, as well as bifidobacteria profiles of the subjects, clustered according to their regions and age. Eight genera were shared among all subjects, however, certain genera distributed differently

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Ali, Ayub, Nazrul Islam, Nazrul Islam Fakir, et al. "The Science Underlying the Probiotic Strain Bifidobacterium in Beneficial Effects on Immunological and Gastrointestinal Health." International Journal of Immunology 12, no. 1 (2024): 10–18. http://dx.doi.org/10.11648/j.iji.20241201.12.

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Probiotics have demonstrated a lot of promise in improving gut health in humans. Despite the encouraging data, nothing is known about the therapeutic effectiveness of many of the probiotics on the market, and it&apos;s sometimes unclear how they work. Humans have long used Bifidobacterium, a well-known, multifunctional probiotic, to treat gastrointestinal, immunological, and infectious disorders. It is also therapeutically useful. This review provides a theoretical framework for comprehending the mechanisms of action of Bifidobacterium and highlights the functional advantages from the most

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