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Artykuły w czasopismach na temat „Biochemical Reactions of Pseudomonas Aeruginosa”

Autor: Grafiati

Data publikacji: 3 czerwca 2025

Data aktualizacji: 31 lipca 2025

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Andrusevich, A. S., M. S. Struk, Y. I. Tsiapsha, О. V. Dubanevich, and G. E. Tolyaronok. "BIOCHEMICAL PROPERTIES OF EPIZOOTIC STRAINS PSEUDOMONAS AERUGINOSA." Ecology and Animal World, no. 2 (December 14, 2022): 27–31. http://dx.doi.org/10.47612/2224-1647-2022-2-27-31.

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The article presents data on the biochemical properties of epizootic strains of Pseudomonas aeruginosa. The belonging of the strains to the species Pseudomonas aeruginosa was confirmed by polymerase chain reaction.

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Hilal, H. A. "Molecular and epidemiological study of pseudomonas aeruginosa isolated from burn patients in Baghdad city-Iraq." CARDIOMETRY, no. 29 (November 1, 2023): 116–21. http://dx.doi.org/10.18137/cardiometry.2023.29.116121.

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Pseudomonas aeruginosa is rod-shaped with Gram-negative pathogens. It causes disease in humans and animals. P. aeruginosa interferes with the burn cure rates and can lead to increased death among the patients. The current study aims to detect pseudomonas aeruginosa isolates using the classical biochemical methods and polymerase chain reaction technique after isolating pseudomonas aeruginosa from burns cases in Baghdad-Iraq. A sterile swab takes Fifty samples randomly from Al-Yarmouk Teaching Hospital and Kadhemia Hospital. All samples were inoculated on rich media and then submitted to stainin

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Wang, Zongwu, Wantao Tian, Siyuan Sun, Xing Chen, and Haifeng Wang. "Genomic and Proteomic Analysis of Pseudomonas aeruginosa Isolated from Industrial Wastewater to Assess Its Resistance to Antibiotics." Separations 10, no. 11 (2023): 549. http://dx.doi.org/10.3390/separations10110549.

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Industrial wastewater usually contains a large amount of organic and inorganic pollutants, and many microorganisms. However, the types of microorganism present in industrial wastewater are still unclear. The aim of this study was to analyze the physicochemical properties and drug resistance of Pseudomonas aeruginosa isolated from industrial wastewater containing high concentrations of sulfate compounds. Pseudomonas aeruginosa was isolated from industrial wastewater from industrial produce with high concentrations of sulfate and phosphate, and mass spectrometry identification, gene identificati

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Patil, Aditi, and Deepashri Naik. "Formation of Biofilms at Surgical Site Infection Caused by Pseudomonas aeruginosa and Their Clinical Significance." International Journal of Health Sciences and Research 14, no. 4 (2024): 542–49. http://dx.doi.org/10.52403/ijhsr.20240469.

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Introduction: Pseudomonas aeruginosa is one of the primary opportunistic pathogens that causes a variety of nosocomial infections.1 The ability of P. aeruginosa to form biofilms in a lot of environments renders antibiotic treatment unproductive and promotes persistent infectious diseases.2 This is a Descriptive and Prospective study to identify formation of biofilms on Surgical sites and to evaluate its effects and significance. Materials & Methods: All swabs from surgical sites were collected from the period February 2021 to January 2022 and were subjected to Standard Microbiological proc

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Rahman, Mizanur, Mohammad Jobayer, Nadira Akter, Farook Ahamed, SM Shamsuzzaman, and Kazi Zulfiquer Mamun. "Rapid detection of Pseudomonad at species level by multiplex PCR in surgical units and ICU of Dhaka Medical College Hospital." Bangladesh Journal of Medical Microbiology 10, no. 2 (2016): 22–26. http://dx.doi.org/10.3329/bjmm.v10i2.51928.

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Pseudomonads are the most important gram negative organisms involved in various types of infection. This cross sectional study was conducted from January to December 2010 to isolate and identify Pseudomonad at species level in different clinical samples by culture and multiplex polymerase chain reaction (PCR) and to evaluate the efficacy of PCR in rapid detection of the bacteria at species level. Wound swab and tips of endotracheal tube were collected from hospitalized patients from different surgical units and intensive care unit (ICU) of Dhaka Medical College Hospital, Dhaka. Pseudomonads we

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A. O. Abdelrahim, Abdalla, I. A. Abdelrhman, M. A. Abdalla, I. M. Fangama, and E. S. Siham. "Isolation and Identification of Food-Borne Bacteria Associated to Beef Carcass from Different Slaughter Houses in Khartoum State Sudan." International Journal of Current Microbiology and Applied Sciences 12, no. 10 (2023): 81–90. http://dx.doi.org/10.20546/ijcmas.2023.1210.010.

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In this investigation a total of 570 bacterial isolates were obtained from 450 beef carcass swab samples collected from different slaughter houses of Khartoum State. According to the cultural characteristics, bacterial morphology and biochemical reactions the identified bacteria were: Escherichia coli (32.3%), Klebsiella pneumoniae (27.2%), Proteus spp (21.2%) and Pseudomonas aeruginosa (19.3%) respectively. E.coli represented the predominant bacteria (32.3%) isolated from swab of beef carcass compared to other bacteria Klebsiella pneumoniae (27.2%), Proteus spp (21.2%), Pseudomonas aeruginosa

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Men, T. T., N. H. Son, T. T. Quynh, et al. "Isolation of bacteria capable of degrading histamine in fermented mackerel (Scomberomorus guttatus)." Food Research 9, no. 2 (2025): 284–90. https://doi.org/10.26656/fr.2017.9(2).124.

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Histamine, a biogenic amine, can be formed in foods via decarbonizing reactions of histidine and can cause health problems in consumers. Thus, histamine levels in foods, especially fermented seafood, are strictly controlled during harvesting, preserving, processing, and delivery. In this study, fermented mackerel (Scomberomorus guttatus) was collected and used to identify histamine-degrading bacteria. Five strains of bacteria grown in histamine medium were isolated, of which two strains (HF1 and HF2) showed the highest biomass in the 50 ppm histamine-supplied medium. After culturing for 24 hrs

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Et al., Ali. "Extraction, Purification and Characterization of Peroxidase from Pseudomonas aeruginosa and Utility as Antioxidant and Anticancer." Baghdad Science Journal 16, no. 4 (2019): 0824. http://dx.doi.org/10.21123/bsj.2019.16.4.0824.

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Peroxidase is a class of oxidation-reduction reaction enzyme that is useful for accelerating many oxidative reactions that protect cells from the harmful effects of free radicals. Peroxidase is found in many common sources like plants, animals and microbes and have extensive uses in numerous industries such as industrial, medical and food processing. In this study, P. aeruginosa was harvested to utilize and study its peroxidases. P. aeruginosa was isolated from a burn patient, and the isolate was verified as P. aeruginosa using staining techniques, biochemical assay, morphological, and a sensi

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Enass Abdul Kadhum Almkhadhree, Kawther Adnan Alqaseer, and Khairi Jameel AL Ruaby. "Molecular detection of virulence genes in pseudomonas aeruginosa isolated from different clinical infections." Journal of the Pakistan Medical Association 73, no. 9 (2023): S62—S66. http://dx.doi.org/10.47391/jpma.iq-13.

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Objective: To isolate pseudomonas aeruginosa from different clinical specimens, and to detect virulence factors.Method: The cross-sectional study was conducted at Al-Zharr Hospital and private clinics in Kut city of Wasit provincein Iraq from October 15, 2021, to April 30, 2022, and comprised samples obtained from different patient sites, such aswound swabs, ear swabs, urine samples, burn swabs and respiratory tract swabs. The samples were subjected tomolecular testing using, among other tools, polymerase chain reaction. The isolates were cultured on different media,such as blood agar, MacConk

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Saha, Tapu Kumar, Ferdousi Begum, SM Lutful Kabir, Md Shafiqul Islam, and Md Shahidur Rahman Khan. "Characterization of bacterial isolates from skin lesions of sheep, goat and cattle in different rearing condition." Asian Journal of Medical and Biological Research 5, no. 2 (2019): 117–25. http://dx.doi.org/10.3329/ajmbr.v5i2.42493.

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The study was performed to isolate and characterize some selected bacteria from skin lesion of sheep, goat and cattle of different rearing condition. Intensive and semi-intensive housing systems were considered for this study. A total of (n=60) skin wound swab and pus samples were collected for this study during the study period. Among them (n=30) samples were collected from intensive farms and (n=30) samples were collected from semi-intensive farms. Staphylococcus aureus infections were found 70% skin lesion for sheep, 30% for goat and 60% for cattle and Pseudomonas aeruginosa infections were

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Abdul-Wahid, Ali A., and Ali M. Almohana. "Dissemination of Aminoglycosides Resistance in Pseudomonas Aeruginosa Isolates in Al-Nasiriya Hospitals Pseudomonas Aeruginosa." Kufa Journal for Nursing Sciences 5, no. 1 (2015): 118–27. http://dx.doi.org/10.36321/kjns.vi20151.3158.

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Background: Pseudomonas Aeruginosa is one of the primary opportunistic pathogens responsible for nosocomial infections. Aminoglycosides are an important component of antipseudomonal chemotherapy. The inactivation of drugs by modifying enzymes and 16S rRNA methylase are the most common mechanisms of aminoglycoside resistance. Aim of the study: Investigate the occurrence of aminoglycoside resistance and the incidence of the 16S rRNA methylase and resistance-modifying enzyme genes in P. Aeruginosa isolated of several hospitals in Al-Nasiriya province. Methodology: A total of 355 clinical isolates

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Hassan,, Ban Ali, and Aseel abdulridha saeed. "Detection and Characterization of Virulent Pseudomonas aeruginosa genes in Fresh Fish at Al-Diwaniya City Market, Iraq." Al-Anbar Journal of Veterinary Sciences 17, no. 1 (2024): 75–83. http://dx.doi.org/10.37940/ajvs.2024.17.1.10.

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Fish meat is more susceptible to pathogen contamination than other protein-rich foodstuffs due to its high deterioration. Pseudomonas aeruginosa is considered one of the most common microbial strains responsible for the deterioration of fish and seafood products. the study's goals are to detecting virulence variables Pseudomonas aeruginosa and estimating the level of contamination in a fish sample, In this research, 120 fresh fish samples, gathered from a the local Diwaniyah city market local fish meat sample. including (60 marine fish and 60 common carp (Cyprinus carpio). The isolates were su

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Sukhada, Buwa, Ingale Hemangi, Bawane Rashmi, Bhandari Sunita, and Sengupta Shubhra. "Antimicrobial Susceptibility Pattern of Pseudomonas aeruginosa Isolates in Clinical Samples with Special Reference to Metallo Beta Lactamase Detection at a Tertiary Care Hospital." International Journal of Toxicological and Pharmacological Research 13, no. 1 (2023): 179–85. https://doi.org/10.5281/zenodo.11311709.

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<strong>Introduction:</strong> In recent years, a significant rise in the incidence of <em>P. aeruginosa</em> as well as multidrug resistance (MDR) has been seen, which has been accompanied by an increase in morbidity and death. The purpose of this study was to ascertain the current status of antimicrobial susceptibility to anti-Pseudomonal drugs and to identify the presence of metallo- beta lactamase (MBL) in these isolates. <strong>Material and Methods: </strong>A prospective study was carried out to obtain <em>P. aeruginosa</em> isolates from various clini

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A. Ali, Aysar, and Enass G. Sweedan. "Role of higB-higA Novel Genes in Antibiotics Resistance of Pseudomonas aeruginosa." INTERNATIONAL JOURNAL OF DRUG DELIVERY TECHNOLOGY 13, no. 02 (2022): 605–9. http://dx.doi.org/10.25258/ijddt.13.2.22.

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Background: Pseudomonas aeruginosa is a devious pathogen with the tendency to prompt many acute and serious chronic diseases. This study aims to detect novel genes (Toxins-Antitoxins II system), especially; higB and higA encoded from P. aeruginosa by PCR technique and the relation between these genes and antibiotic resistance of P. aeruginosa. Methods: This study detected 50 isolates of P. aeruginosa from distinct clinical sources. The most common origin of isolates was (44%) burn swabs, (22%) urine culture, (12%) wound swabs, (14%) sputum, and (8%) ear swabs. The bacteria were isolated using

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Edan, Dawood Salim, and Nahi Yousif Yaseen. "Studying the Incidence of Pseudomonas Species as A Causative Agent of Otitis in Iraqi Patients." Al Mustansiriyah Journal of Pharmaceutical Sciences 14, no. 1 (2014): 136–42. http://dx.doi.org/10.32947/ajps.v14i1.138.

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Otitis can affect the inner or outer parts of the ear. Some times it is difficult in a rapid response to the treatment. The condition is classified according to occured suddenly for a short time (acute) or repeatedly over a long period of time (chronic).The types of treatments andsustainability were depended in most cases on bacterial infections.23 samples of Iraqi patients infected with otitis collected from Yarmook hospital. The aural exudates screened to determine whether the infection was external or interior ear on MacConkey agar, and the negative Gram bacteria has been selected due to it

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Hasan, Alamgir, Md Tanjir Ahmmed, Bushra Benta Rahman Prapti, et al. "First report of MDR virulent Pseudomonas aeruginosa in apparently healthy Japanese quail (Coturnix japonica) in Bangladesh." PLOS ONE 20, no. 1 (2025): e0316667. https://doi.org/10.1371/journal.pone.0316667.

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Pseudomonas aeruginosa (P. aeruginosa) is a major pathogen associated conditions like septicaemia, respiratory disorders, and diarrhoea in poultry, particularly in Japanese quail (Coturnix japonica). The infection causes huge economical losses due to its high transmissibility, mortality and zoonotic potential. This study aimed to isolate, identify, detect virulence genes, and profile multidrug resistance (MDR) of P. aeruginosa from Japanese quail. Oral and rectal swabs were collected from 110 apparently healthy quail birds across various districts in Bangladesh. Bacterial isolation and identif

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R Hussein, Khwam. "Molecular feature of lasB gene of Pseudomonas aeruginosa isolated from different clinical sources." University of Thi-Qar Journal of Science 6, no. 4 (2018): 163–66. http://dx.doi.org/10.32792/utq/utjsci/v6i4.91.

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The study was carried out through a period from June to November 2017 from out-patients and in-patients of Al-Hussein Teaching Hospital in Thi-Qar province South of Iraq. A sum of 132 specimens from the hospital facility condition and different clinical samples of various patients were randomly collected and analyzed for diagnosis of Pseudomonas aeruginosa. These clinical specimens were included wound, burn, ear swabs, urine and sputum specimens. Every single specimen was screened to detect of P. aeruginosa by culturing on suitable media, and 36 isolates of P. aeruginosa were distinguished thr

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Azam, Saima, Mohammad Zahid Mustafa, Muhammad Kamran Taj, Muhammad Azam Mengal, Muhammad Alam Mengal, and Ashiq Hussain. "Drug susceptibility, Prevalence and effect of Pseudomonas aeruginosa on wound healing from burn patients." Journal of the Pakistan Medical Association 74, no. 1 (2023): 67–71. http://dx.doi.org/10.47391/jpma.8477.

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Objective: To determine the prevalence, antibiotic susceptibility and effect of Pseudomonas aeruginosa in relation to burn patients. Method: The cross-sectional study was conducted at the Centre for Advanced Studies in Vaccinology and Biotechnology, University of Balochistan, Quetta, Pakistan, from March 2018 to May 2021, and comprised pus swab cultures were isolated from inpatients with 2nd and 3rd degree burns aged up to 60 years at Bolan Medical Complex Hospital and Sandeman Provinical Hospital, the two main government tertiary care hospitals in Quetta. The samples were immediately cultured

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Raheema, Rana H., Sabaa Hilal Hadi, and Zainab Adil Ghani Chabuck. "Study of some virulence genes from uropathogenic Klebsiella pneumoniae and Pseudomonas aeruginosa isolated in Wasit Province, Iraq." Medical Journal of Babylon 21, no. 1 (2024): 207–13. http://dx.doi.org/10.4103/mjbl.mjbl_1100_23.

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Abstract Background: Klebsiella pneumoniae and Pseudomonas aeruginosa are the most common causes of nosocomial urinary tract infections (UTIs), and in recent years, these bacteria have become significantly more resistant to antibiotics. Objective: The objective of the study was to determine the frequency of uropathogenic K. pneumoniae and P. aeruginosa in clinical urine samples from patients with UTIs in Wasit Province. Materials and Methods: Initially, 100 clinical urine samples were taken for this investigation from (October 1 to December 1, 2022), and the isolates were initially identified

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Journal, Baghdad Science. "Detection of Pseudomonas aeruginosa in Clinical Samples Using PCR Targeting ETA and gyrB Genes." Baghdad Science Journal 15, no. 4 (2018): 401–5. http://dx.doi.org/10.21123/bsj.15.4.401-405.

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Pseudomonas aeruginosa has variety of virulence factors that contribute to its pathogenicity. Therefore, rapid detection with high accuracy and specificity is very important in the control of this pathogenic bacterium. To evaluate the accuracy and specificity of Polymerase Chain Reaction (PCR) assay, ETA and gyrB genes were targeted to detect pathogenic strains of P. aeruginosa. Seventy swab samples were taken from patients with infected wounds and burns in two hospitals in Erbil and Koya cities in Iraq. The isolates were traditionally identified using phenotypic methods, and DNA was extracted

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Yang, Zhe, and Chung-Dar Lu. "Characterization of an Arginine:Pyruvate Transaminase in Arginine Catabolism of Pseudomonas aeruginosa PAO1." Journal of Bacteriology 189, no. 11 (2007): 3954–59. http://dx.doi.org/10.1128/jb.00262-07.

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ABSTRACT The arginine transaminase (ATA) pathway represents one of the multiple pathways for l-arginine catabolism in Pseudomonas aeruginosa. The AruH protein was proposed to catalyze the first step in the ATA pathway, converting the substrates l-arginine and pyruvate into 2-ketoarginine and l-alanine. Here we report the initial biochemical characterization of this enzyme. The aruH gene was overexpressed in Escherichia coli, and its product was purified to homogeneity. High-performance liquid chromatography and mass spectrometry (MS) analyses were employed to detect the presence of the transam

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Bakir, Sevan H., Fattma A. Ali, Bashdar M. Hussen, Shahin H. Mustafa, Ahmed Akil, and Shilan S. Ahmad. "Occurrence of blaTEM among Pseudomonas aeruginosa Strains Isolated from Different Clinical Samples in Erbil City." Polytechnic Journal 11, no. 2 (2021): 87–94. http://dx.doi.org/10.25156/ptj.v11n2y2021.pp87-94.

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Pseudomonas aeruginosa was a significant source of nosocomial infection with the presence of extended-spectrum β-lactamases (ESBLs) and metallo-β-lactamases (MBL) genes in P. aeruginosa being progressively documented globally. The purpose of this investigation was to detection the occurrence of MBL and ESBL in P. aeruginosa isolates acquired from diverse clinical samples, as well as the prevalence of blaTEM genes producing ESBLs. A total of 227 samples were obtained from various clinical specimens (wound, urine, sputum, bronchial wash, and burn) at public hospitals in Erbil region. Microorgani

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Adeyemi, Folasade M., Rashidat R. Adeboye, Adetoun A. Adebunmi, Nana-Aishat Yusuf, and Abideen A. Wahab. "Detection of T3SS, oprI, aprA, and pvdA Genes in Clinical Isolates of Pseudomonas aeruginosa obtained from Wound Samples." Pan African Journal of Life Sciences 4, no. 1 (2020): 17–24. http://dx.doi.org/10.36108/pajols/0202/40(0140).

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Background: Pseudomonas aeruginosa employs a varied number o f virulence determinants, predominantly porins, type III secretion system (T3SS), alkaline protease and pigments to manipulate the host to establish infections. These factors contribute significantly to virulence in P. aeruginosa and are worrisome. This study is aimed at identifying the virulence genes in P. aeruginosa isolates from wound swabs of patients at two tertiary hospitals in Osun State, Nigeria. Methods: Altogether , 237 participants consisting of 133 fr o m State Hospital, Osogbo and 104 from General Hospital, Iwo with dif

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Younus, Mustafa D., Omar F. Bahjat, and Sirwan A. Rashid. "Antibiotic Susceptibility Pattern, Molecular Characterization of Virulence Genes among Pseudomonas Aeruginosa Isolated from Burn Patients." Cihan University-Erbil Scientific Journal 5, no. 1 (2021): 36–41. http://dx.doi.org/10.24086/cuesj.v5n1y2021.pp36-41.

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Abstract In this research a total of 150 samples were obtained from burn and wound patients admitted to the West Erbil Emergence Hospital during period from September 2020 to January 2021. Through cultural, morphological features, biochemical testing and Vitek’s 2 compact systems, 40 isolates of P. aeruginosa have been identified. P. aeruginosa produced various pigments, including blue / green, and yellow / green. The iso1ates of P. aeruginosa were subjected to 14 different antibiotics. Impenim was the most effective antimicrobial agents against all P. aerugionsa isolates, and most of is

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Mahtuti, Erni Yohani, and Farahdita Devi Masyitoh. "Isolasi dan Identifikasi Mikroba Berpotensi Pendegradasi Limbah Cair Laboratorium Kesehatan STIKes Maharani Malang." BIOSAINTROPIS (BIOSCIENCE-TROPIC) 5, no. 1 (2019): 38–44. http://dx.doi.org/10.33474/e-jbst.v5i1.227.

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Laboratory waste produced unique characteristics, contrast to waste produced by industrial activities. Material waste that comes from the laboratory has greater variety of waste types; although the amount of material discarded is not many. The research objective was to obtain bacterial isolates that were able to survive in laboratory waste as potential waste-degrading bacteria. Research method is observartional laboratory with isolate reaction testing that was detected by the ability to degrade starch, cellulose, proteins and non-organic compounds. The sampling method was purposive sampling. T

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Wadi, Fatima Rheem, and Maryam Kareem Ali. "Molecular Detection of Psts Antibiotic-Resistant Gene in Pseudomonas aeruginosa Isolates." Medical Journal of Babylon 21, no. 2 (2024): 481–84. http://dx.doi.org/10.4103/mjbl.mjbl_792_23.

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Abstract Background: Otitis media is a very common infection among people of different age groups. Objective: The goal of the current work was to determine the existence of PstS gene among Pseudomonas aeruginosa isolates recovered from otitis media cases in Iraqi patients. Materials and Methods: A total of 100 ear swabs were collected from patients suffering from otitis media attending the ENT outpatient clinic at Baghdad Teaching Hospital from July 2022 to the end of December 2022. The age range was 18-75 years. Detection of morphology and biochemical testing by Vitek 2 system. The antibiotic

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Westman, Erin L., David J. Mcnally, Martin Rejzek та ін. "Identification and biochemical characterization of two novel UDP-2,3-diacetamido-2,3-dideoxy-α-D-glucuronic acid 2-epimerases from respiratory pathogens". Biochemical Journal 405, № 1 (2007): 123–30. http://dx.doi.org/10.1042/bj20070017.

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The heteropolymeric O-antigen of the lipopolysaccharide from Pseudomonas aeruginosa serogroup O5 as well as the band-A trisaccharide from Bordetella pertussis contain the di-N-acetylated mannosaminuronic acid derivative, β-D-ManNAc3NAcA (2,3-diacetamido-2,3-dideoxy-β-D-mannuronic acid). The biosynthesis of the precursor for this sugar is proposed to require five steps, through which UDP-α-D-GlcNAc (UDP-N-acetyl-α-D-glucosamine) is converted via four steps into UDP-α-D-GlcNAc3NAcA (UDP-2,3-diacetamido-2,3-dideoxy-α-D-glucuronic acid), and this intermediate compound is then epimerized by WbpI (P

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Bridier, A., F. Dubois-Brissonnet, G. Greub, V. Thomas, and R. Briandet. "Dynamics of the Action of Biocides in Pseudomonas aeruginosa Biofilms." Antimicrobial Agents and Chemotherapy 55, no. 6 (2011): 2648–54. http://dx.doi.org/10.1128/aac.01760-10.

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ABSTRACTThe biocidal activity of peracetic acid (PAA) and benzalkonium chloride (BAC) onPseudomonas aeruginosabiofilms was investigated by using a recently developed confocal laser scanning microscopy (CLSM) method that enables the direct and real-time visualization of cell inactivation within the structure. This technique is based on monitoring the loss of fluorescence that corresponds to the leakage of a fluorophore out of cells due to membrane permeabilization by the biocides. Although this approach has previously been used with success with various Gram-positive species, it is not directly

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Sajjad, Ayesha, Muna Malik, Iffat Javed, Sohaila Mushtaq, Fareeha Imran, and Rabiya Jameel. "Detection of metallo beta lactamase production in imipenem resistant gram negative bacilli non fermenters isolated in a Tertiary Care Hospital." Professional Medical Journal 26, no. 12 (2019): 2080–84. http://dx.doi.org/10.29309/tpmj/2019.26.12.3073.

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Objectives: Metallo-beta lactamase (MBL) producing non fermenter Gram negative bacilli is an emerging warning and cause of worry as they have established as one of the most feared resistance mechanisms and are the foremost cause of nosocomial infections worldwide. Carbapenem, including Imipenem, Meropenem and Doripenem are often used as a last remedy for treatment of infections caused by Pseudomonas aeruginosa, Acinetobacter and other Gram-negative. The present study was designed to explore the distribution of imipenem resistant non-fermenter Gram-negative bacilli isolates in different age gro

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Umejiuba, Nancy Oluchukwu, E. S. I. Okorondu, E. C. Chinakwe, et al. "Diversity and Enzymatic Activity Profile of Bacteria Isolated from Selected Organic Wastes in Owerri, Imo State, Nigeria." UMYU Journal of Microbiology Research (UJMR) 9, no. 2 (2024): 80–90. https://doi.org/10.47430/ujmr.2492.009.

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Study’s Novelty/Excerpt: Enzyme activity of bacteria isolated from organic waste samples (cow dung, municipal solid waste, poultry, and pig waste) is profiled. 832 bacterial isolates were identified based on their cultural, microscopic, and biochemical properties. Enterococcus faecalis is found to be the most prevalent (20.1%). Pseudomonas aeruginosa is the most active enzyme (protease, amylase, cellulase, etc.) producer. Shigella spp. showed no enzyme production. Full Abstract: Enzymes secreted by bacteria are bio-catalysts that play an important role in all stages of metabolism and biochemic

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Guerra-Renteria, A. Suggey, M. Alberto García-Ramírez, César Gómez-Hermosillo, Abril Gómez-Guzmán, Yolanda González-García, and Orfil González-Reynoso. "Metabolic Pathway Analysis of Nitrogen and Phosphorus Uptake by the Consortium between C. vulgaris and P. aeruginosa." International Journal of Molecular Sciences 20, no. 8 (2019): 1978. http://dx.doi.org/10.3390/ijms20081978.

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Anthropogenic activities have increased the amount of urban wastewater discharged into natural aquatic reservoirs containing a high amount of nutrients such as phosphorus (Pi and PO 4 − 3 ), nitrogen (NH 3 and NO 3 − ) and organic contaminants. Most of the urban wastewater in Mexico do not receive any treatment to remove nutrients. Several studies have reported that an alternative to reduce those contaminants is using consortiums of microalgae and endogenous bacteria. In this research, a genome-scale biochemical reaction network is reconstructed for the co-culture between the microalga Chlorel

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Gondha, Dr Suhani, Dr Ghanshyam Kavathia, and Dr Arpita Bhattacharya. "A Study of Isolation, Identification & Antibiotic Susceptibility Pattern of Non-Fermenting Gram Negative Bacilli Isolated From Various Clinical Samples at Tertiary Care Hospital Rajkot, Gujarat, Western India." Saudi Journal of Pathology and Microbiology 7, no. 6 (2022): 233–39. http://dx.doi.org/10.36348/sjpm.2022.v07i06.002.

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Introduction: Nonfermenting gram-negative bacilli (NFGNB) are a taxonomically diverse group of aerobic, nonsporing, bacilli that either do not utilize glucose as a source of energy or utilize it oxidatively. They occur as saprophytes in the environment and some are also found as commensals in the human gut. In recent year NFGNB have emerged as important health care associated pathogens. They have been incriminated in infections such as bacteremia, meningitis, pneumonia, urinary tract infections, surgical site infections, wound infections, osteomyelitis etc. Materials and method: During Nine Mo

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Qader, Govend Musa, Khanzad Khudhur Jarjees, and Rozhhalat Khudhur Jarjees. "Molecular detection of Metallo-Beta-Lactamase and alginate in multidrug resistance Pseudomonas aeruginosa isolated from the clinical specimen." Journal of Medicine and Life 15, no. 9 (2022): 1105–9. http://dx.doi.org/10.25122/jml-2021-0196.

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Pseudomonas aeruginosa pathogen is opportunistic. Several virulence factors and biofilms can cause its pathogenicity. Furthermore, infections triggered via multidrug-resistant P. aeruginosa among hospitalized patients are a public health concern. The primary antimicrobial agents in treating Gram-negative infection include Meropenem and Imipenem. Moreover, the spread of Carbapenem-resistant P. aeruginosa is a focal concern worldwide. The present research aims to determine the spread of Carbapenem-resistant P. aeruginosa, and the distribution of the Alginate and Metallo-beta-lactamase encoding g

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Al-Daghistani, Hala I., Lubna F. Abu-Niaaj, and Sima Zein. "Accurate Diagnosis of Pseudomonas aeruginosa Is Critical to Mitigating Development of Antibiotic Resistance." Antibiotics 14, no. 5 (2025): 509. https://doi.org/10.3390/antibiotics14050509.

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Background: The accurate and rapid diagnosis of infections is critical for effective and timely treatment. Misdiagnosis often leads to the prescription of antibiotics not targeting the causing agent of infection and thus be the possible development of multidrug resistance. This collectively worsens the condition and might lead to unnecessary intervention or death. The abundance of Pseudomonas spp. in healthcare-settings and the environment may lead to the inaccurate diagnosis of P. aeruginosa, making the treatment of its infections challenging. P. aeruginosa is a Gram-negative, opportunistic p

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Burkaltseva, Maria V., A. V. Lazareva, E. A. Pleteneva, et al. "Imaging of the bacterial interactions in lung co-infection in cystic fibrosis patients." Clinical Microbiology and Antimicrobial Chemotherapy 22, no. 2 (2020): 155–60. http://dx.doi.org/10.36488/cmac.2020.2.155-160.

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Objective. To identify bacterial interactions at the site of infection in cystic fibrosis patients and to assess their possible effects on the course of infection. Materials and Methods. The following strains were used in this study: Alcaligenes faecalis LGBP strain, isolated from the environment; clinical isolates of Pseudomonas aeruginosa; Achromobacter xylosoxidans, Acinetobacter baumannii, Alcaligenes faecalis, and Bacillus subtilis strains; the standard laboratory P. aeruginosa PAO1 strain and its lysogens by temperate bacteriophages of various species, and its phageresistant mutants. Ima

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Abdul Razzaq, Asif Hasan. "Bacteriological and molecular study of Pseudomonas aeruginosa strains isolated from different clinical cases in Erbil and Kurkuk." Iraqi Journal of Veterinary Medicine 41, no. 2 (2018): 124–30. http://dx.doi.org/10.30539/iraqijvm.v41i2.61.

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This study included isolates of bacteria from 125 clinical samples in Erbil and Kirkuk Hospital including (burns, wounds, urine and sputum); 38 isolates were identified as P. aeruginosa after conducting microscopic and biochemical tests. The results of antibiotic sensitivity test showed that all isolates of P. aeruginosa were different in resistance to Pipracillin, Erythromycin with rate of (100%) and to the Nalidixic acid (94.73%) while the lowest resistant antibiotics were to Co-trimoxazole, Ceftazidime and Ciprofloxacin, which amounted to (26.31%, 23.68 and 21.05%) respectively. For molecul

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Ashraf Ayyal Mutar Alrashedi. "Identification Of Metallo?-Lactamses and Integron Genes In Pseudomonas Aeruginosa Isolated From Burn Injury Patients: Phenotype And Genotype." International Science of Health Journal 2, no. 4 (2024): 01–12. http://dx.doi.org/10.59680/ishel.v2i4.1457.

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Pseudomonas aeruginosa, which generates metallo-?-lactamase (MBL), is the cause of infections linked to burn patients, and this is a growing global problem. The objectives of the recent study were to determine antibiotic susceptibility of P. aeruginosa isolates, the presence of MBLs genes, and Integron gene class-1 . One hundred burn patients at the Al-Hussein Teaching Hospital in Al Smawah, Iraq, were isolated clinically. Twenty (20%) of these were determined to be P. aeruginosa by 16sRNA and biochemical testing. To further identify the antibiotic sensitivity for each isolate was employed by

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Shehu, Rabi, Samson Baranzan Wayah, and Jonathan Maiangwa. "Evaluation of the roles of Mex genes in antibiotic resistance of some strains of <i>Pseudomonas aeruginosa</i> and the use of antibiotic combinations to boost their susceptibility pattern." Dutse Journal of Pure and Applied Sciences 10, no. 4c (2025): 1–13. https://doi.org/10.4314/dujopas.v10i4c.1.

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Pseudomonas aeruginosa is a prevalent pathogen responsible for various infections, and exhibits significant resistance to a broad spectrum of antibiotics, leading to increased mortality rates. This study aimed to assess the role of mex genes in antibiotic resistance among indigenous strains of Pseudomonas aeruginosa and enhance their susceptibility through antibiotic combinations. Ten isolates were selected based on their appearance on selective media, microscopy and biochemical characterization. Antibiotic resistance profiles for fluoroquinolones (ofloxacin, pefloxacin, sparfloxacin, ciproflo

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Aman, Bibi Sazain, Muhammad Kamran Taj, Imran Taj, Saima Azam, Sakina Khan, and Rozina Rashid. "The bacterial profile and antibiotic susceptibility in skin and soft tissue infections at tertiary care hospital of Quetta, Pakistan." Journal of the Pakistan Medical Association 74, no. 7 (2024): 1249–54. http://dx.doi.org/10.47391/jpma.10261.

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Objective: To determine the bacterial profile and antibiotic susceptibility in skin and soft tissue infections among patients in a tertiary care setting. Method: The cross-sectional cohort study was conducted at the Centre for Advanced Studies in Vaccinology and Biotechnology, University of Balochistan, Quetta, Pakistan, from June 2021 to May 2022, and comprised bacteria-infected skin samples that were collected from the Bolan Medical Complex Hospital, Quetta, and the Sandeman Provincial Hospital, Quetta. The swab samples were immediately cultured, and positive samples were evaluated for bioch

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Mahmood, Ahmed N. "Isolation and identification of pseudomonas aeruginosa from infected sheep and detection of phosolipase C (lecithinase." Iraqi Journal of Veterinary Medicine 39, no. 1 (2015): 28–32. http://dx.doi.org/10.30539/iraqijvm.v39i1.193.

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The current study dealt with the isolation and identification of 19 isolates (14.07%) for the period (October 2013 to January 2014) from different samples (135 samples) number of isolates belonging to Pseudomonas aeruginosa were 8 isolated from urine samples, their percentage were 33.33 % and 5 isolates from nasal swabs, (11.11%) and 4 isolated from milk samples (9.30%), 2 isolated from wound swabs (24%) and isolates from eye infection (9.09%). These isolates were identified by morphological examination and biochemical tests and API-20 NE system. The second part was the study of the antibiotic

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LIN, MIAO CHU, AY HUEY HUANG, HAU YANG TSEN, HIN-CHUNG WONG, and TSUNG CHAIN CHANG. "Use of Oligonucleotide Array for Identification of Six Foodborne Pathogens and Pseudomonas aeruginosa Grown on Selective Media." Journal of Food Protection 68, no. 11 (2005): 2278–86. http://dx.doi.org/10.4315/0362-028x-68.11.2278.

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Identification of presumptive foodborne pathogens grown on selective media may take one to several days and requires a different battery of biochemical tests for each microorganism. A molecular identification method was developed in which universal primers were used to amplify the 16S to 23S rDNA intergenic spacer of target microorganisms, and PCR products were hybridized to a panel of species-specific oligonucleotides that were immobilized on a nylon membrane. The seven target microorganisms were Bacillus cereus, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella, St

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Begum, Most Fahmida, Mahfuza Nasrin, Rezina Karim, and Mohammad Shah Alam. "Bacteriological profile and antibiotic susceptibility patterns of wound infections at Uttara Adhunik Medical College Hospital, Dhaka." Bangladesh Journal of Medical Microbiology 14, no. 1 (2020): 15–19. http://dx.doi.org/10.3329/bjmm.v14i1.57762.

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Background and objectives: Wound infection is one of the most important causes of morbidity and mortality worldwide and antibiotic resistant bacteria are a great part of complications in treatment of the infection. The present study was conducted to isolate and identify the etiological agents of wound infection and to assess the antimicrobial susceptibility pattern of the isolates. Methodology: The retrospective study was carried out on wound infection suspected patients for six months duration. The collected pus specimens were first observed macroscopically then streaked on MacConkey ag

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Tadjrobehkar, Omid, and Atefeh Kamali. "Evaluation of Antibiotic Resistance Pattern and Extended-Spectrum Beta-lactamases in Pseudomonas aeruginosa Isolates Obtained from Clinical Samples by Phenotypic and Genotypic Methods in Zabol, Iran." Journal of Kerman University of Medical Sciences 29, no. 6 (2022): 529–35. http://dx.doi.org/10.34172/jkmu.2022.64.

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Background: Pseudomonas aeruginosa is a human opportunistic pathogen that is known to be responsible for various diseases. However, its antibiotic-resistant isolates often cause serious infections. Methods: This study for the first time investigated a total of 80 P. aeruginosa isolates collected from patients admitted to Amir Al-Momenin hospital. The isolates were identified by biochemical assays. The combination disc test method was used to measure antibiotic susceptibility and confirm the presence of extended spectrum-beta lactamases-producing enzymes. Also, the presence of enzyme-producing

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Novgorodova, O. Ju, M. F. Starodub та V. O. Ushkalov. "Підготовка компонентів селективної поверхні трансдюсера імунного біосенсора для індикації Pseudomonas aeruginosa". Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 19, № 77 (2017): 190–93. http://dx.doi.org/10.15421/nvlvet7741.

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The article presents the results of research to obtain specific components of the preparation selective biosensor surface for further development the immune biosensor test-system for the express – indication of bacteria Pseudomonas aeruginosa and their detection in biological material and in the environment. Specific antiserum and immunoglobulins to P. aeruginosa were obtained by autors. The resulting surface modification components for activity and specificity of the specific compounents were tested using analytical device imunosensor «Plasmon-6», based on surface plasmon resonance. Biosensor

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Abdulla, Roaa Abd Al-Rahman, and Rasmiya Abd Aburesha. "Gene Expression Evaluation of mexT and mexF Genes of Pseudomonas aeruginosa under Antibiotic Stress." Medical Journal of Babylon 22, no. 1 (2025): 282–88. https://doi.org/10.4103/mjbl.mjbl_776_23.

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Abstract Background: Pseudomonas aeruginosa is a major cause of mortality and morbidity in those with immunodeficiencies and those who have cystic fibrosis and antibiotic resistance. Objectives: To investigate the role of genes responsible for pump efflux of antibiotics (mexF and mexT) and their relation to antibiotic resistance. Materials and Methods: A total of 100 clinical swabs were collected from different hospitals in Baghdad, Iraq. The identification of bacterial isolates was confirmed using the VITEK2 compact system and 16s rRNA. Drug susceptibility tests were performed by the VITEK2 c

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Firdaus, Maulidan, Desy Nila Rahmana, Diah Fitri Carolina, Nisrina Rahma Firdausi, Zulfaa Afiifah, and Berlian Ayu Rismawati Sugiarto. "Potential Applications of Antibacteria in Edible Coating as Fish Preservative." Natural Science and Advanced Technology Education 30, no. 5 (2021): 454–69. http://dx.doi.org/10.53656/nat2021-5.02.

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Fish is a product that breaks down quickly due to biochemical reactions that cause a decrease in the quality of its nutritional and sensory values. Natural preservatives make fish safer for consumption than fish preserved with formalin. Edible coating is a preservative that can be eaten, can prevent biological, chemical and physical changes, is able to prevent mass transfer, acts as a moisture barrier, be contained antimicrobial/antibacterial and antioxidants, increases shelf life, as well as protects food from microbial contamination. Antimicrobial/antibacterial compounds added to edible coat

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Rout, Swikrutee, Rahul Kumar, and Kundan Kumar Sahu. "Molecular identification of Pseudomonas aeruginosa isolated from burn patients by polymerase chain reaction in central Odisha, India." Journal of Integrative Medicine and Research 3, no. 2 (2025): 109–16. https://doi.org/10.4103/jimr.jimr_9_25.

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Background: Burn wound infections pose significant challenges, leading to severe complications and increased mortality. Pseudomonas aeruginosa is a major pathogen in burn patients, known for biofilm formation and multidrug resistance. Rapid identification of bacterial pathogens is crucial for effective treatment and infection control. Traditional microbiological methods may lack speed and accuracy for timely interventions. Molecular techniques such as polymerase chain reaction (PCR) provide high specificity and sensitivity in detecting nosocomial infections. Polymicrobial infections complicate

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Anjum, Hasnain, Md Shamsul Arefin, Nusrat Jahan, et al. "Roles of intrinsic and acquired resistance determinants in multidrug-resistant clinical Pseudomonas aeruginosa in Bangladesh." Bangladesh Journal of Medical Science 22, no. 3 (2023): 489–507. http://dx.doi.org/10.3329/bjms.v22i3.66960.

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Introduction: Pseudomonas aeruginosa is an ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, P. aeruginosa, and Enterobacter spp.) pathogen and one of the leading etiologies in multiple nosocomial infections. Treatment of P. aeruginosa is becoming increasingly difficult due to its ever-increasing antibiotic resistance trends. This study investigated clinical multidrug resistance (MDR) P. aeruginosa (MDR-PA), their intrinsic resistance determinants, including the presence of chromosomal AmpC β-lactamase (Ampicillinase), decreased expression of

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Ramachandran, Devasena Umai, and Meenambiga Setti Sudharsan. "Molecular Characterization of Antibiotic Resistant Genes among Gram Negative Clinical Isolates." Trends in Sciences 19, no. 4 (2022): 2687. http://dx.doi.org/10.48048/tis.2022.2687.

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Increasing antibiotic resistance is a threat worldwide. Deep studies have to be done for correct and appropriate usage of antibiotics for treating infections efficiently and to curtail the spread of resistance. Hence, this study is aimed at understanding the prevalence of antibiotic resistance genes among 35 gram-negative bacterial isolates. By performing biochemical tests, the isolates were identified to be Escherichia coli (n = 15), Klebsiella oxytoca (n = 3), Klebsiella pneumoniae (n = 5), Proteus mirabilis (n = 4), Proteus vulgaris (n = 1) and Pseudomonas aeruginosa (n = 7). Antibiotic sus

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Afoshin, Alexey, Svetlana Tishchenko, Azat Gabdulkhakov та ін. "Structural and Functional Characterization of β−lytic Protease from Lysobacter capsici VKM B−2533T". International Journal of Molecular Sciences 23, № 24 (2022): 16100. http://dx.doi.org/10.3390/ijms232416100.

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The crystal structure of the Lysobacter capsici VKM B−2533T β-lytic protease (Blp), a medicinally promising antimicrobial enzyme, was first solved. Blp was established to possess a folding characteristic of the M23 protease family. The groove of the Blp active site, as compared with that of the LasA structural homologue from Pseudomonas aeruginosa, was found to have amino acid differences. Biochemical analysis revealed no differences in the optimal reaction conditions for manifesting Blp and LasA bacteriolytic activities. At the same time, Blp had a broader range of action against living and a

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