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Rozprawy doktorskie na temat „Biomolecular analyses”

Autor: Grafiati

Data publikacji: 3 czerwca 2025

Data aktualizacji: 31 lipca 2025

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1

Göransson, Jenny. "Readout Strategies for Biomolecular Analyses." Doctoral thesis, Uppsala universitet, Institutionen för genetik och patologi, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-9343.

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This thesis describes three readout formats for molecular analyses. A common feature in all works is probing techniques that upon specific target recognition ideally results in equimolar amounts of DNA circles. These are then specifically amplified and detected using any of the techniques presented herein. The first paper presents a method that enables homogeneous digital detection and enumeration of biomolecules, represented as fluorescence-labelled DNA macromolecules. This method offers precise measurements to be performed with a wide linear dynamic range. As an application, two closely rela

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Göransson, Jenny. "Readout Strategies for Biomolecular Analyses /." Uppsala : Acta Universitatis Upsaliensis, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-9343.

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3

Lin, Xiu-Mei [Verfasser], Volker [Akademischer Betreuer] Deckert, and Wolfgang [Akademischer Betreuer] Weigand. "Tip-enhanced Raman Spectroscopy (TERS) for Biomolecular Analyses at Nanometer / Xiu-Mei Lin. Gutachter: Volker Deckert ; Wolfgang Weigand." Jena : Thüringer Universitäts- und Landesbibliothek Jena, 2014. http://d-nb.info/1047579073/34.

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Lin, Xiu-Mei [Verfasser], Volker Akademischer Betreuer] Deckert, and Wolfgang [Akademischer Betreuer] [Weigand. "Tip-enhanced Raman Spectroscopy (TERS) for Biomolecular Analyses at Nanometer / Xiu-Mei Lin. Gutachter: Volker Deckert ; Wolfgang Weigand." Jena : Thüringer Universitäts- und Landesbibliothek Jena, 2014. http://nbn-resolving.de/urn:nbn:de:gbv:27-20140210-112607-2.

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5

Navarrete, Belda Vanessa. "Domesticación animal y primeras prácticas ganaderas en el noreste peninsular (5500-4500 cal BC). Integración de los análisis bioquímicos en arqueozoología." Doctoral thesis, Universitat Autònoma de Barcelona, 2017. http://hdl.handle.net/10803/461194.

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En el presente trabajo de tesis doctoral se aborda la problemática de la dinámica del proceso de domesticación animal y las prácticas ganaderas iniciales en el extremo más occidental del área mediterránea a lo largo del intervalo temporal que abarca entre el 5.500-4.700 cal ANE. El objetivo principal de la tesis se aborda a partir del estudio de una muestra significativa de yacimientos neolíticos situados en el noreste de la Península Ibérica. Se ha evaluado con esta tesis doctoral los principales modelos explicativos planteados en torno a los orígenes del neolítico en la Península Ibérica,

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6

Zhang, Yingbo. "Microfluidic methods for biomolecular analysis." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/274931.

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Microfluidics is the science and technology of manipulating fluids at small scales ranging from microlitres (10$^{-6}$) to picolitres (10$^{-12}$). The fundamental physics is distinct from fluid behaviour on bulk scales and laminar flow is the key characteristic on this scale. Microfluidic systems have a wide range of applications in many disciplines from engineering to physics, chemistry and biotechnology. In this thesis, I explore different strategies exploiting the capabilities of microfluidic devices for manipulating and analysing biomolecules. A particular focus of the work is on the stud

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7

Tiwari, Purushottam Babu. "Multimode Analysis of Nanoscale Biomolecular Interactions." FIU Digital Commons, 2015. http://digitalcommons.fiu.edu/etd/1923.

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Biomolecular interactions, including protein-protein, protein-DNA, and protein-ligand interactions, are of special importance in all biological systems. These interactions may occer during the loading of biomolecules to interfaces, the translocation of biomolecules through transmembrane protein pores, and the movement of biomolecules in a crowded intracellular environment. The molecular interaction of a protein with its binding partners is crucial in fundamental biological processes such as electron transfer, intracellular signal transmission and regulation, neuroprotective mechanisms, and reg

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8

Heucke, Stephan F. "Advancing nanophotonic devices for biomolecular analysis." Diss., Ludwig-Maximilians-Universität München, 2013. http://nbn-resolving.de/urn:nbn:de:bvb:19-165294.

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9

Malleo, Daniele. "Impedance spectroscopy for cellular and biomolecular analysis." Thesis, University of Southampton, 2009. https://eprints.soton.ac.uk/66388/.

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The application of microfabrication technology to molecular and cell biology has motivated the rapid development of a novel class of microdevices collectively known as ‘Lab On a Chip’ devices. Impedance spectroscopy is a non-invasive, label-free, analytical technique that is amenable to miniaturization, electronic integration, automation, and scalability within ‘LOC’ devices. This thesis describes a microfabricated device for performing continuous impedance analysis of individual cells held in an array of hydrodynamic traps. This device enables continuous long-term analysis of cells and time-d

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10

Ezzeddine, Bilal. "Instrumentation for multilinear analysis of biomolecular fluorescence /." The Ohio State University, 1989. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487671640054913.

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11

Ivanov, Aleksandar P. "Novel nanopore/nanoelectrode architectures for biomolecular analysis." Thesis, Imperial College London, 2012. http://hdl.handle.net/10044/1/9506.

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The main focus of this work is the development of a solid‐state nanopore detector with embedded tunnelling electrodes capable of label‐free detection and ultimately sequencing of individual DNA molecules. In such a device a pair of tunnelling electrodes is precisely aligned to the nanopore opening. DNA molecules are electrophoretically driven through the nanopore and ‘read’ at the tunnelling junction. In this thesis a technique based on electron beam induced deposition (EBID) is being explored and developed for the precise fabrication of tunnelling electrodes that are aligned to a nanopore. Fu

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12

Oesterheld, Matthias. "Analysis of biomolecular networks using a generic network analysis suite." kostenfrei, 2008. http://mediatum2.ub.tum.de/doc/646475/646475.pdf.

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13

Harwood, Thomas. "The use of terahertz spectroscopy for biomolecular analysis." Thesis, University of Strathclyde, 2016. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=27058.

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Terahertz radiation occupies part of the electromagnetic spectrum between the mid-infraredand microwave bands (approximately 0.1 – 20 THz). Within this range, spectroscopic analysis of biological molecules can detect low-frequency vibrational modes that arise from intermolecular as well as intramolecular interactions and can provide significant information regarding structure and function. For example, the spectra of small biomolecules is dominated by individual modes and hydrogen bonds that can produce a distinctive spectral ‘fingerprint’,whereas the spectra of macromolecules, such as protein

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14

Pereira, Fiona Marie. "Continuous and segmented-flow microfluidics for biomolecular analysis." Thesis, Imperial College London, 2012. http://hdl.handle.net/10044/1/9302.

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Segmented flows in microfluidics have attracted much recent attention. Of particular interest is the use of nanolitre droplets as interface tools in microfluidics. A droplet interface connects techniques while leaving them sufficiently independent of each other; a task that difficult to perform within a continuous flow system. The first part of this thesis is dedicated to sample preparation and analysis in continuous flow microfluidic systems. The polymerase chain reaction (PCR) is investigated, using electroosmotic flow to transport the sample across various temperature zones. Additionally, a

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15

Anderson, Henrik. "Development of Electroacoustic Sensors for Biomolecular Interaction Analysis." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-107211.

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16

Yang, Yinhua, and 楊銀花. "Application of biomolecular NMR spectroscopy for protein structure determination." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B42182013.

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17

Zhou, Xiamo. "OSTE Microfluidic Technologies for Cell Encapsulation and Biomolecular Analysis." Doctoral thesis, KTH, Mikro- och nanosystemteknik, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-217995.

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In novel drug delivery system, the encapsulation of therapeutic cells in microparticles has great promises for the treatment of a range of health con- ditions. Therefore, the encapsulation material and technology are of great importance to the validity and efficiency of the advanced medical therapy. Several unsolved challenges in regards to versatile microparticle synthesis ma- terials and methods form the main obstacle for a translation of novel cell therapy concepts from research to clinical practice. Thiol-ene based polymer systems have emerged and gained great popular- ity in material deve

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18

Connors, Wendy Lee. "Sequential injection analysis for the investigation of biomolecular interactions /." Thesis, Connect to this title online; UW restricted, 2000. http://hdl.handle.net/1773/8481.

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19

Tecle, Tesfaldet. "Biomolecular characterization of mumps virus genotypes with varying neurovirulence /." Stockholm : [Karolinska institutets bibl.], 2002. http://diss.kib.ki.se/2002/91-7349-234-5.

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20

Ericsson, Olle. "Biomolecular Analysis by Dual-Tag Microarrays and Single Molecule Amplification." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Universitetsbiblioteket [distributör], 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-8475.

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21

Silva, Santisteban Tomas [Verfasser], and Matthias [Akademischer Betreuer] Meier. "Spheroid manipulation on a microfluidic chip platform for biomolecular analysis." Freiburg : Universität, 2017. http://d-nb.info/1144829658/34.

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22

Liao, Tao. "Geometric Modeling and Shape Analysis for Biomolecular Complexes Based on Eigenfunctions." Research Showcase @ CMU, 2015. http://repository.cmu.edu/dissertations/655.

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Geometric modeling of biomolecules plays an important role in the study of biochemical processes. Many simulation methods depend heavily on the geometric models of biomolecules. Among various studies, shape analysis is one of the most important topics, which reveals the functionalities of biomolecules.

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23

Krone, Michael [Verfasser], and Thomas [Akademischer Betreuer] Ertl. "Interactive visual analysis of biomolecular simulations / Michael Krone ; Betreuer: Thomas Ertl." Stuttgart : Universitätsbibliothek der Universität Stuttgart, 2016. http://d-nb.info/1118369181/34.

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24

SENIGAGLIESI, BEATRICE. "Biophysical and biomolecular analysis of EVs and their interaction with target cells." Doctoral thesis, Università degli Studi di Trieste, 2021. http://hdl.handle.net/11368/2988324.

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Triple negative breast cancer (TNBC) is one of the most aggressive breast cancer subtype and with a poor prognosis. Nowadays, chemotherapy is the main treatment in both early and advanced stage of the TNBC, but patients without complete response to conventional chemotherapy are approximately 80%. In light of that, clarifying biological mechanisms of the metastatic process is crucial in finding new therapeutic approaches for effective interventions. Metastasis is thought to be easier for more deformable and, therefore, soft cancer cells, which can migrate through narrow pores of extracellular m

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25

Wang, Congzhou. "INTEGRATED NANOSCALE IMAGING AND SPATIAL RECOGNITION OF BIOMOLECULES ON SURFACES." VCU Scholars Compass, 2015. http://scholarscompass.vcu.edu/etd/4067.

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Biomolecules on cell surfaces play critical roles in diverse biological and physiological processes. However, conventional bulk scale techniques are unable to clarify the density and distribution of specific biomolecules in situ on single, living cell surfaces at the micro or nanoscale. In this work, a single cell analysis technique based on Atomic Force Microscopy (AFM) is developed to spatially identify biomolecules and characterize nanomechanical properties on single cell surfaces. The unique advantage of these AFM-based techniques lies in the ability to operate in situ (in a non-destructiv

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26

Saha, Saumitra. "Spectral and orbital based analyses of interactions in some biomolecules /." Swinburne Research Bank, 2008. http://hdl.handle.net/1959.3/46099.

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Thesis (Ph. D.)--Swinburne University of Technology, Centre for Molecular Simulation - 2008.<br>Dissertation submitted in fulfilment of requirements for the degree Doctor of Philosophy, Centre for Molecular Simulation, Swinburne University of Technology, 2008. Typescript. Includes bibliographical references (p. 188-218).

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27

Roberts, Carl. "Spatio-temporal analysis of changes of shape for constituent bodies within biomolecular aggregates." Thesis, University of East Anglia, 2012. https://ueaeprints.uea.ac.uk/40464/.

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Changes of shape are important in many situations of interest in biology at different typical length scales. Approaches for modelling the behaviour of droplets in suspension and thermallydriven motion of the molecular chains in enzymes are presented. Both models use orthogonal basis functions to describe the spatial dependences in a spherical geometry. Both models also describe the effect of time-dependent boundary data on the shape of the bodies involved, a stochastic response for the enzyme model (dimensions of the order 10−9 m) and smooth response for the colloidal model (dimensions of the

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28

Chan, Ho Man. "Analysis of biomolecules by total internal reflection fluorescence microscopy." HKBU Institutional Repository, 2011. http://repository.hkbu.edu.hk/etd_ra/1254.

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29

Karki, Santosh. "Laser Electrospray Mass Spectrometry for Structural Analysis of Biomolecules." Diss., Temple University Libraries, 2017. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/434619.

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Chemistry<br>Ph.D.<br>This dissertation elucidates a greater understanding of protein folding and unfolding processes during the lifetimes of electrospray and nano-spray droplets in laser electrospray mass spectrometry (LEMS) and nano-laser electrospray mass spectrometry (nano-LEMS) measurements, respectively. The similarity in mass spectral features obtained from conventional electrospray measurements for supercharged proteins with those of LEMS measurements suggested that supercharging phenomena occurs in the electrospray droplets during the droplet desolvation process. It was observed that

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30

Olmo, Stefano <1978&gt. "Capillary coatings in Capillary Electrophoresis (CE) analysis of Biomolecules." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2008. http://amsdottorato.unibo.it/1071/1/Tesi_Olmo_Stefano.pdf.

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31

Olmo, Stefano <1978&gt. "Capillary coatings in Capillary Electrophoresis (CE) analysis of Biomolecules." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2008. http://amsdottorato.unibo.it/1071/.

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32

Pate, Martin Eric. "A practical investigation into the use of principal component analysis for the modelling and scale-up of high performance liquid chromatography." Thesis, University College London (University of London), 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.322003.

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33

Nielsen, Robert D. "An analysis of continuous wave and time domain electron paramagnetic resonance spectra with applications to biological systems /." Thesis, Connect to this title online; UW restricted, 2003. http://hdl.handle.net/1773/8596.

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34

Pregibon, Daniel Colin. "Enabling technologies for multiplexed biomolecule analysis and cell sorting." Thesis, Massachusetts Institute of Technology, 2008. http://hdl.handle.net/1721.1/43215.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 2008.<br>This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.<br>Includes bibliographical references (p. [109]-122).<br>The quantification and manipulation of biological entities from a physiological sample is extremely important for a broad range of applications in medical diagnostics, therapeutics, and basic science research. From a diagnostics standpoint, the cells, proteins, and nucleic acids that compose our bo

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35

Sampson, Dayle Lorand. "Urinary biomolecular indicators of exercise-induced over exertion injury." Thesis, Queensland University of Technology, 2013. https://eprints.qut.edu.au/62158/1/Dayle_Sampson_Thesis.pdf.

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Poor health and injury represent major obstacles to the future economic security of Australia. The national economic cost of work-related injury is estimated at $57.5 billion p/a. Since exposure to high physical demands is a major risk factor for musculoskeletal injury, monitoring and managing such physical activity levels in workers is a potentially important injury prevention strategy. Current injury monitoring practices are inadequate for the provision of clinically valuable information about the tissue specific responses to physical exertion. Injury of various soft tissue structures can

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36

Triulzi, Robert C. "Nanotechnology for Molecular Recognition of Biological Analytes." Scholarly Repository, 2009. http://scholarlyrepository.miami.edu/oa_dissertations/195.

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Nanotechnology is a term used to describe nanometer scaled systems. This thesis presents various nanomaterials and systems for the investigation of biologically relevant analytes in general, and in particular for their detection, decontamination, or destruction. The validation of short peptide fragments as models for protein aggregation is initially discussed through applying spectroscopic and microscopic techniques to Langmuir monolayer surface chemistry. Following this validation, the use of nanogold as a photoablative material for the destruction of aggregated protein is investigated. S

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37

Sandén, Tor. "Analysis of Fluorescence Flicker as a Tool to Monitor Proton Transport and Biomolecular Interactions." Licentiate thesis, KTH, Physics, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4216.

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<p>The overall focus of this thesis is on fluorescence flicker processes of fluorescent molecules, e.g. protonation-deprotonation or singlet-triplet electronic state transitions, intrinsic or generated by their interaction with their environment, monitored by fluorescence spectroscopy.</p><p>Understanding proton migration along membranes and membrane proteins in cells is essential for understanding energy metabolism. It has been seen that certain membrane-spanning proton-transporter proteins in the respiratory chain in the mitochondrial inner membrane take up protons faster than the rate limit

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38

Sandén, Tor. "Analysis of fluorescence flicker as a tool to monitor proton transport and biomolecular interactions /." Stockholm : School of Engineering Sciences, Royal Institute of Technology, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4216.

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39

Lenive, Oleg. "The role of extrinsic noise in biomolecular information processing systems : an in silico analysis." Thesis, Imperial College London, 2014. http://hdl.handle.net/10044/1/31575.

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The intrinsic stochasticity of biomolecular systems is a well studied phe- nomenon. Less attention has been paied to other sources of variability, so called extrinsic noise. While the precise definition of extrinsic noise de- pends on the system in question, it affects all cells and its significance has been demonstrated experimentally. Information theory provides a rigorous mathematical framework for quan- tifying both the amount of information available to a signalling system and its ability to transmit this information. Intracellular signal transduction re- mains a relatively unexplored fro

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40

Delmotte, Antoine Pierre Valere. "All-scale structural analysis of biomolecules through dynamical graph partitioning." Thesis, Imperial College London, 2014. http://hdl.handle.net/10044/1/29861.

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From femtosecond bond vibrations to millisecond domain motions, the dynamics of biomolecules spans a wide range of time and length scales. This hierarchy of overlapping scales links the molecular and biophysical details to key aspects of their functionality. However, the span of scales combined with their intricate coupling rapidly drives atomic simulation methods to their limits, thereby often resulting in the need for coarse-graining techniques which cannot take full account of the biochemical details. To overcome this tradeoff, a graph-theoretical framework inspired by multiscale community

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41

Shou, Wilson Zhenyu. "Fundamental study of electrospray mass spectrometry and its application in inorganic/organometallic species." Diss., Georgia Institute of Technology, 1999. http://hdl.handle.net/1853/30962.

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42

Marlin, Nicola Dawn. "Novel stationary phases for the analysis of biomolecules by capillary electrochromatography." Thesis, Imperial College London, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.414475.

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43

Cowcher, David Paul. "The development of enhanced Raman scattering for the trace analysis of biomolecules." Thesis, University of Manchester, 2014. https://www.research.manchester.ac.uk/portal/en/theses/the-development-of-enhanced-raman-scattering-for-the-trace-analysis-of-biomolecules(0cf1158e-ff5f-4841-a679-16cde8ba0d94).html.

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Raman spectroscopy is an established analytical technique for determining molecular structure, whose major drawback is lack of sensitivity. Enhanced Raman scattering techniques, such as surface-enhanced Raman scattering (SERS) and tip-enhanced Raman scattering (TERS), utilise nanoscale substrates to enhance the Raman signal through the interaction of surface charges with the incident electromagnetic radiation. Here, nanoparticle-based SERS was used to detect dipicolinic acid (DPA), a biomarker for bacterial spores. Whilst this has been demonstrated previously, the use of a different nanopartic

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44

Jia, Wei Jie. "The application of MALDI and tandem reflectron TOF mass spectrometry to the analysis of biomolecular ions." Thesis, University of Glasgow, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.321839.

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45

Lin, Tzu-Jen. "Force Field Parameters and Atomistic Surface Models forHydroxyapatite and Analysis of Biomolecular Adsorption at Aqueous Interfaces." University of Akron / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=akron1365511604.

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46

Sweeney, Deacon John. "A Computational Tool for Biomolecular Structure Analysis Based On Chemical and Enzymatic Modification of Native Proteins." Wright State University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=wright1316440232.

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47

Xia, Yang. "Chromatography coupled with mass spectrometry in bioanalysis." Diss., Georgia Institute of Technology, 1999. http://hdl.handle.net/1853/27998.

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48

Taylor, Ryan M. "Bioinformatic Solutions to Complex Problems in Mass Spectrometry Based Analysis of Biomolecules." BYU ScholarsArchive, 2014. https://scholarsarchive.byu.edu/etd/5585.

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Biological research has benefitted greatly from the advent of omic methods. For many biomolecules, mass spectrometry (MS) methods are most widely employed due to the sensitivity which allows low quantities of sample and the speed which allows analysis of complex samples. Improvements in instrument and sample preparation techniques create opportunities for large scale experimentation. The complexity and volume of data produced by modern MS-omic instrumentation challenges biological interpretation, while the complexity of the instrumentation, sample noise, and complexity of data analysis pres

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49

Xu, Di. "Multifunctional cyanine fluorophores for cellular imaging and sensing in vivo and beta-amyloid imaging and aggregation inhibition." HKBU Institutional Repository, 2017. https://repository.hkbu.edu.hk/etd_oa/382.

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The development of facile and reliable methods to image and detect important biomolecules has drawn considerable attention owing to their potential applications in clinical, bioanalytical and forensic analysis. One-photon microscopy (OPM) has traditionally been used in cell biology research. However, probes based on OPM are associated with shortcomings including photobleaching, cell damage, and intracellular autofluorescence interference. Many researchers are seeking better tools to overcome these obstacles. Two-photon microscopy (TPM) is a convenient and powerful tool to explore the intracell

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50

Chen, Zuofeng, and 陳作鋒. "Studies on a novel type of electrogenerated chemiluminescence and electroanalysis of biomolecules at fluorosurfactant-modifiedelectrodes." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B41634196.

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