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1

Hochstrate, P., and K. Hamdorf. "Microvillar components of light adaptation in blowflies." Journal of General Physiology 95, no. 5 (May 1, 1990): 891–910. http://dx.doi.org/10.1085/jgp.95.5.891.

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The process of light adaptation in blowfly photoreceptors was analyzed using intracellular recording techniques and double and triple flash stimuli. Adapting flashes of increasing intensity caused a progressive reduction in the excitability of the photoreceptors, which became temporarily suppressed when 3 x 10(6) quanta were absorbed by the cell. This suppression was confirmed by subsequently applying an intense test flash that photoactivated a considerable fraction of the 10(8) visual pigment molecules in the cell. The period of temporary desensitization is referred to as the refractory perio
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2

Komo, Larissa, and Damien Charabidze. "Balance between larval and pupal development time in carrion blowflies." Journal of Insect Physiology 133 (August 2021): 104292. http://dx.doi.org/10.1016/j.jinsphys.2021.104292.

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Fukushi, Tsukasa. "Visual learning in walking blowflies,Lucilia cuprina." Journal of Comparative Physiology A 157, no. 6 (November 1985): 771–78. http://dx.doi.org/10.1007/bf01350074.

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Campbell, H. R. "Orientation discrimination independent of retinal matching by blowflies." Journal of Experimental Biology 204, no. 1 (January 1, 2001): 15–23. http://dx.doi.org/10.1242/jeb.204.1.15.

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Blowflies, Phaenicia sericata, can be trained to discriminate in a learning paradigm in which one of the two visual cues is positively rewarded. Retinotopic matching of a learned visual image to the same retinal location from viewing to viewing has been hypothesized to underlie visual pattern learning and memory in insects. To address the theory of retinotopic matching, a detailed analysis was made of the flies' body orientations during learned discriminations between +45 degrees and −45 degrees gratings. Initial approaches to the positive rewarded visual cue did not originate from the same sp
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5

Blaj, G., and J. H. van Hateren. "Saccadic head and thorax movements in freely walking blowflies." Journal of Comparative Physiology A 190, no. 11 (July 20, 2004): 861–68. http://dx.doi.org/10.1007/s00359-004-0541-4.

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Liscia, A. "Taste Modulators are Tools to Gain a Better Insight into Specific Sensitivity of Chemoreceptors in Blowflies." Chemical Senses 30, Supplement 1 (January 1, 2005): i279—i280. http://dx.doi.org/10.1093/chemse/bjh223.

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7

Park, K. C., and A. Cork. "Electrophysiological responses of antennal receptor neurons in female Australian sheep blowflies, Lucilia cuprina, to host odours." Journal of Insect Physiology 45, no. 1 (January 1999): 85–91. http://dx.doi.org/10.1016/s0022-1910(98)00102-4.

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8

Stavenga, D. G., P. B. W. Schwering, and J. Tinbergen. "A THREE-COMPARTMENT MODEL DESCRIBING TEMPERATURE CHANGES IN TETHERED FLYING BLOWFLIES." Journal of Experimental Biology 185, no. 1 (December 1, 1993): 325–33. http://dx.doi.org/10.1242/jeb.185.1.325.

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A three-compartment model is presented that describes temperature measurements of tethered flying blowflies, obtained by thermal imaging. During rest, the body temperature is approximately equal to the ambient temperature. At the start of flight, the thorax temperature increases exponentially with a time constant of 30 s; in steady flight, a temperature of approximately 30°C is reached (ambient temperature approximately 25°C). After flight, the temperature of the thorax decreases exponentially with a time constant of 50 s. Fitting the time courses of the three body compartments, i.e. head, tho
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9

Ouyang, Qin, Hiroyasu Sato, Yoshihiro Murata, Atsushi Nakamura, Mamiko Ozaki, and Tadashi Nakamura. "Contribution of the inositol 1,4,5-trisphosphate transduction cascade to the detection of “bitter” compounds in blowflies." Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 153, no. 3 (July 2009): 309–16. http://dx.doi.org/10.1016/j.cbpa.2009.03.004.

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10

Hainsworth, F. R., G. Fisher, and E. Precup. "Rates of energy processing by blowflies: the uses for a joule vary with food quality and quantity." Journal of Experimental Biology 150, no. 1 (May 1, 1990): 257–68. http://dx.doi.org/10.1242/jeb.150.1.257.

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Data on the variation of crop volumes with time for blowflies (Phormia regina Meigen) fed various volumes and concentrations of fructose or sucrose (from Gelperin, 1966, and Edgecomb et al. 1987) were used to characterize energy processing rates to test the assumption of food energy addivity of optimal foraging theories. Six regression models (linear, square root, cube root, hyperbolic, inverse cube root and exponential) were compared for data from Edgecomb et al. (1987) with measurements of crop volumes from 10 min to 5 h after blowflies were fed 9.7 or 14.5 microliters of 0.25 moll-1 sucrose
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11

Busse, F. K., and R. H. Barth. "Physiology of feeding-preference patterns of female black blowflies (Phormia regina Meigen): Modification in responsiveness to salts subsequent to salt feeding." Journal of Insect Physiology 31, no. 1 (January 1985): 23–26. http://dx.doi.org/10.1016/0022-1910(85)90037-x.

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12

EDGECOMB, ROBERT S., ANGELA R. PYLE, and LARRY L. MURDOCK. "The Role of Water in Tarsal Taste Thresholds to Sugar in the Blowfly Phormia Regina." Journal of Experimental Biology 142, no. 1 (March 1, 1989): 245–55. http://dx.doi.org/10.1242/jeb.142.1.245.

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Thirsty blowflies whose tarsi come into contact with water will respond with proboscis extension. When flies were exposed to high or low relative humidities (RH) prior to testing, the tarsal taste thresholds to sucrose in flies that were unresponsive to water alone increased or decreased, respectively. Evidently, thirst can also be subthreshold and recognizable only as a change in threshold to aqueous sucrose. Thresholds to sucrose and fructose, which were in the millimolar range in 3-day-old adult flies held at 55–70% RH prior to testing, fell significantly after the flies had been held at lo
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13

Boeddeker, N. "A single control system for smooth and saccade-like pursuit in blowflies." Journal of Experimental Biology 208, no. 8 (April 15, 2005): 1563–72. http://dx.doi.org/10.1242/jeb.01558.

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14

EDGECOMB, ROBERT S., LARRY L. MURDOCK, ANDRÉ B. SMITH, and MARGARET D. STEPHEN. "Regulation of Tarsal Taste Threshold in the Blowfly, Phormia Regina." Journal of Experimental Biology 127, no. 1 (January 1, 1987): 79–94. http://dx.doi.org/10.1242/jeb.127.1.79.

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The nutritional condition of adult blowflies (Phormia regina Meigen) affects their readiness to respond with proboscis extension when their tarsi contact food stimuli. Thresholds are high in sated flies (100–1000 mmol−1 sucrose) and low in starved flies (1–10 mmoll−1 sucrose). Two feeding regimes employing aqueous sucrose were used to reveal factors regulating tarsal taste threshold in this insect: long-term feeding (ad libitum) and single meals administered to starved flies. A positive logarithmic relationship was found between crop weight and tarsal taste threshold, expressed as mean accepta
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15

El-Wadawi, R., and K. Bowler. "The development of thermotolerance protects blowfly flight muscle mitochondrial function from heat damage." Journal of Experimental Biology 198, no. 11 (November 1, 1995): 2413–21. http://dx.doi.org/10.1242/jeb.198.11.2413.

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The LD50 of 10-day-old adult blowflies was determined to be 38.12±0.07 °C. A transitory increase in heat resistance occurred following the exposure of adult blowflies to a sublethal heat shock at 36 °C. This thermotolerance was apparent 1 h after the application of the shock, was maximal 2­3 h later and had disappeared 6 h after exposure. Oxidative phosphorylation by flight muscle mitochondria from control flies was impaired by an LD50 dose in vivo using both pyruvate+proline (P+P) and glycerol 3-phosphate (G3P) as substrates. Acceptor control (state III resp
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16

Liang, Pei, Roland Kern, Rafael Kurtz, and Martin Egelhaaf. "Impact of visual motion adaptation on neural responses to objects and its dependence on the temporal characteristics of optic flow." Journal of Neurophysiology 105, no. 4 (April 2011): 1825–34. http://dx.doi.org/10.1152/jn.00359.2010.

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It is still unclear how sensory systems efficiently encode signals with statistics as experienced by animals in the real world and what role adaptation plays during normal behavior. Therefore, we studied the performance of visual motion-sensitive neurons of blowflies, the horizontal system neurons, with optic flow that was reconstructed from the head trajectories of semi-free-flying flies. To test how motion adaptation is affected by optic flow dynamics, we manipulated the seminatural optic flow by targeted modifications of the flight trajectories and assessed to what extent neuronal responses
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17

Warzecha, A., W. Horstmann, and M. Egelhaaf. "Temperature-dependence of neuronal performance in the motion pathway of the blowfly calliphora erythrocephala." Journal of Experimental Biology 202, no. 22 (November 15, 1999): 3161–70. http://dx.doi.org/10.1242/jeb.202.22.3161.

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Raising the head temperature within a behaviourally relevant range has strong effects on the performance of an identified neuron, the H1 neuron, in the visual motion pathway of blowflies. The effect is seen as an increase in the mean amplitude of the responses to motion under both transient and steady-state conditions, a considerable decrease in the response latency and an improvement in the reliability of the responses to motion. These temperature-dependent effects are independent of whether the animal is exposed to transient temperature changes or is maintained continuously at the same tempe
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18

Campbell, H. R., and N. J. Strausfeld. "Learned discrimination of pattern orientation in walking flies." Journal of Experimental Biology 204, no. 1 (January 1, 2001): 1–14. http://dx.doi.org/10.1242/jeb.204.1.1.

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To determine the pattern-orientation discrimination ability of blowflies, Phaenicia sericata, a learning/memory assay was developed in which sucrose served as the reward stimulus and was paired with one of two visual gratings of different orientations. Individual, freely walking flies with clipped wings were trained to discriminate between pairs of visual patterns presented in the vertical plane. During training trials, individual flies learned to search preferentially at the rewarded stimulus. In subsequent testing trials, flies continued to exhibit a learned preference for the previously rew
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19

Berrigan, D., and J. R. Lighton. "Bioenergetic and kinematic consequences of limblessness in larval Diptera." Journal of Experimental Biology 179, no. 1 (June 1, 1993): 245–59. http://dx.doi.org/10.1242/jeb.179.1.245.

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We report the cost of transport and kinematics of terrestrial locomotion by larval blowflies (Protophormia terraenovae, Diptera: Calliphoridae). We contrast inter- and intra-individual methods for estimating minimum cost of transport (MCOT) and the relationship between speed, contraction frequency and distance traveled per contraction. The minimum cost of transport calculated from intra-individual data is 2297 +/− 317 J kg-1 m-1 (S.E.M.) and the MCOT calculated from inter-individual comparisons is statistically indistinguishable at 1910 +/− 327 J kg-1 m-1. These values are almost ten times hig
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20

Meglič, Andrej, and Gregor Zupančič. "Changes in redox states of respiratory pigments recorded from the eyes of live blowflies exposed to light stimuli and hypoxia." Journal of Comparative Physiology A 197, no. 3 (December 1, 2010): 301–10. http://dx.doi.org/10.1007/s00359-010-0612-7.

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21

Hateren, J. H., and C. Schilstra. "Blowfly flight and optic flow. II. Head movements during flight." Journal of Experimental Biology 202, no. 11 (June 1, 1999): 1491–500. http://dx.doi.org/10.1242/jeb.202.11.1491.

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The position and orientation of the thorax and head of flying blowflies (Calliphora vicina) were measured using small sensor coils mounted on the thorax and head. During flight, roll movements of the thorax are compensated by counter rolls of the head relative to the thorax. The yaw turns of the thorax (thorax saccades) are accompanied by faster saccades of the head, starting later and finishing earlier than the thorax saccades. Blowfly flight can be divided into two sets of episodes: ‘during saccades’, when high angular velocities of up to a few thousand degrees per second are reached by both
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22

Simmons, P., S. Jian, and F. Rind. "CHARACTERISATION OF LARGE SECOND-ORDER OCELLAR NEURONES OF THE BLOWFLY CALLIPHORA ERYTHROCEPHALA." Journal of Experimental Biology 191, no. 1 (June 1, 1994): 231–45. http://dx.doi.org/10.1242/jeb.191.1.231.

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1. Blowflies have twelve large, second-order ocellar neurones (L-neurones) with axons in the single ocellar nerve. These neurones have fairly restricted arborizations in the posterior slope neuropile of the protocerebrum and cell bodies in the nerve, near to the fused ocellar retinae. 2. Like ocellar L-neurones of other insects, or large second-order neurones of the fly compound eye, blowfly L-neurones hyperpolarise in response to increases in light intensity and depolarise in response to decreases in light intensity. Both polarities of response have a strong phasic component. Adaptation to su
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23

Lent, C. M., and M. H. Dickinson. "On the termination of ingestive behaviour by the medicinal leech." Journal of Experimental Biology 131, no. 1 (September 1, 1987): 1–15. http://dx.doi.org/10.1242/jeb.131.1.1.

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Hungry leeches, Hirudo medicinalis, ingest blood meals averaging 890% of their mass in 29 min. Ingestion is terminated as a result of distension of the body: experimentally distending leeches as they feed causes an immediate cessation of ingestion and inhibits any subsequent biting behaviour; if distension is circumvented by various experimental procedures, leech ingestive periods are prolonged significantly. Ingestion is not terminated as a result of fatigue, chemical cues or mass change. Distension also underlies satiation, for removing blood from the crops of recently fed leeches qualitativ
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24

Warzecha, A. K., M. Egelhaaf, and A. Borst. "Neural circuit tuning fly visual interneurons to motion of small objects. I. Dissection of the circuit by pharmacological and photoinactivation techniques." Journal of Neurophysiology 69, no. 2 (February 1, 1993): 329–39. http://dx.doi.org/10.1152/jn.1993.69.2.329.

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1. Visual interneurons tuned to the motion of small objects are found in many animal species and are assumed to be the neuronal basis of figure-ground discrimination by relative motion. A well-examined example is the FD1-cell in the third visual neuropil of blowflies. This cell type responds best to motion of small objects. Motion of extended patterns elicits only small responses. As a neuronal mechanism that leads to such a response characteristic, it was proposed that the FD1-cell is inhibited by the two presumably GABAergic and, thus, inhibitory CH-cells, the VCH- and the DCH-cell. The CH-c
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25

Schilstra, C., and J. H. Hateren. "Blowfly flight and optic flow. I. Thorax kinematics and flight dynamics." Journal of Experimental Biology 202, no. 11 (June 1, 1999): 1481–90. http://dx.doi.org/10.1242/jeb.202.11.1481.

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The motion of the thorax of the blowfly Calliphora vicina was measured during cruising flight inside a cage measuring 40 cmx40 cmx40 cm. Sensor coils mounted on the thorax picked up externally generated magnetic fields and yielded measurements of the position and orientation of the thorax with a resolution of 1 ms, 0.3 degrees and 1 mm. Flight velocities inside the cage were up to 1.2 m s-1, and accelerations were up to 1 g (approximately 10 m s-2) vertically and 2 g horizontally. During flight, blowflies performed a series of short (approximately 20–30 ms) saccade-like turns at a rate of appr
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26

Nässel, D. R., M. Y. Kim, and C. T. Lundquist. "Several forms of callitachykinins are distributed in the central nervous system and intestine of the blowfly Calliphora vomitoria." Journal of Experimental Biology 198, no. 12 (December 1, 1995): 2527–36. http://dx.doi.org/10.1242/jeb.198.12.2527.

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We have examined the distribution of two tachykinin-related neuropeptides, callitachykinin I and II (CavTK-I and CavTK-II), isolated from whole-animal extracts of the blowfly Calliphora vomitoria. Extracts of dissected brains, thoracic-abdominal ganglia and midguts of adult blowflies and the entire central nervous system of larval flies were analysed by high performance liquid chromatography (HPLC) combined with enzyme-linked immunosorbent assay (ELISA) for the presence of CavTKs. To identify the two neuropeptides by HPLC, we used the retention times of synthetic CavTK-I and II as reference an
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27

Beckers, Ulrich, Martin Egelhaaf, and Rafael Kurtz. "Synapses in the Fly Motion–Vision Pathway: Evidence for a Broad Range of Signal Amplitudes and Dynamics." Journal of Neurophysiology 97, no. 3 (March 2007): 2032–41. http://dx.doi.org/10.1152/jn.01116.2006.

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Synapses are generally considered to operate efficiently only when their signaling range matches the spectrum of prevailing presynaptic signals in terms of both amplitudes and dynamics. However, the prerequisites for optimally matching the signaling ranges may differ between spike-mediated and graded synaptic transmission. This poses a problem for synapses that convey both graded and spike signals at the same time. We addressed this issue by tracing transmission systematically in vivo in the blowfly's visual-motion pathway by recording from single neurons that receive mixed potential signals c
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28

Yan, Liping, Thomas Pape, Karen Meusemann, Sujatha Narayanan Kutty, Rudolf Meier, Keith M. Bayless, and Dong Zhang. "Monophyletic blowflies revealed by phylogenomics." BMC Biology 19, no. 1 (October 27, 2021). http://dx.doi.org/10.1186/s12915-021-01156-4.

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Abstract Background Blowflies are ubiquitous insects, often shiny and metallic, and the larvae of many species provide important ecosystem services (e.g., recycling carrion) and are used in forensics and debridement therapy. Yet, the taxon has repeatedly been recovered to be para- or polyphyletic, and the lack of a well-corroborated phylogeny has prevented a robust classification. Results We here resolve the relationships between the different blowfly subclades by including all recognized subfamilies in a phylogenomic analysis using 2221 single-copy nuclear protein-coding genes of Diptera. Max
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29

Stavenga, D. G., R. Kruizinga, and H. L. Leertouwer. "Dioptrics of the facet lenses of male blowflies Calliphora and Chrysomyia." Journal of Comparative Physiology A 166, no. 3 (February 1990). http://dx.doi.org/10.1007/bf00204809.

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Monteagudo, José, Martin Egelhaaf, and Jens Peter Lindemann. "Random attention can explain apparent object choice behavior in free-walking blowflies." Journal of Experimental Biology, March 29, 2022. http://dx.doi.org/10.1242/jeb.243801.

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Flies are often observed to approach dark objects. To a naïve observer they seem to pay selective attention to one out of several objects although previous research identified as a possible underlying mechanism a reflex-like fixation behavior integrating responses to all objects. In a combination of behavioral experiments and computational modelling, we investigated the choice behavior of flies freely walking towards an arrangement of two objects placed at a variable distance from each other. The walking trajectories were oriented towards one of the objects much earlier than predicted by a sim
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31

Hickner, Paul V., Omprakash Mittapalli, Anjana Subramoniam, Agustin Sagel, Wes Watson, Maxwell J. Scott, Alex P. Arp, Adalberto A. Pérez de León, and Zainulabeuddin Syed. "Physiological and molecular correlates of the screwworm fly attraction to wound and animal odors." Scientific Reports 10, no. 1 (November 27, 2020). http://dx.doi.org/10.1038/s41598-020-77541-w.

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AbstractThe screwworm fly, Cochliomyia hominivorax (Coquerel), was successfully eradicated from the United States by the sterile insect technique (SIT). However, recent detection of these flies in the Florida Keys, and increased risk of introductions to the other areas warrant novel tools for management of the flies. Surveillance, a key component of screwworm control programs, utilizes traps baited with rotting liver or a blend of synthetic chemicals such as swormlure-4. In this work, we evaluated the olfactory physiology of the screwworm fly and compared it with the non-obligate ectoparasitic
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32

Supple, Jack A., Léandre Varennes-Phillit, Dexter Gajjar-Reid, Uroš Cerkvenik, Gregor Belušič, and Holger G. Krapp. "Generating spatiotemporal patterns of linearly polarised light at high frame rates for insect vision research." Journal of Experimental Biology, June 16, 2022. http://dx.doi.org/10.1242/jeb.244087.

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Polarisation vision is commonplace among invertebrates; however, most experiments focus on determining behavioural and/or neurophysiological responses to static polarised light sources rather than moving patterns of polarised light. To this end, we designed a polarisation stimulation device based on superimposing polarised and non-polarised images from two projectors, which can display moving patterns at frame rates exceeding invertebrate flicker fusion frequencies. A linear polariser fitted to one projector enables moving patterns of polarised light to be displayed, whilst the other projector
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33

Thyselius, Malin, Yuri Ogawa, Richard Leibbrandt, Trevor J. Wardill, Paloma T. Gonzalez-Bellido, and Karin Nordström. "Hoverfly (Eristalis tenax) pursuit of artificial targets." Journal of Experimental Biology, January 25, 2023. http://dx.doi.org/10.1242/jeb.244895.

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The ability to visualize small moving objects is vital for the survival of many animals, as these could represent predators or prey. For example, predatory insects, including dragonflies, robber flies and killer flies, perform elegant, high-speed pursuits of both biological and artificial targets. Many non-predatory insects, including male hoverflies and blowflies, also pursue targets during territorial or courtship interactions. To date, most hoverfly pursuits were studied outdoors. To investigate naturalistic hoverfly (Eristalis tenax) pursuits under more controlled settings, we constructed
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