Gotowe bibliografie tematyczne / Bp DNA fragment

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Gotowa bibliografia na temat „Bp DNA fragment”

Autor: Grafiati
Data publikacji: 3 czerwca 2025
Data aktualizacji: 31 lipca 2025

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Artykuły w czasopismach na temat "Bp DNA fragment"

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Lan, Vo Thi Thuong, and Le Thi Thanh. "Construction of DNA ladder for determination of small size DNA fragments." Vietnam Journal of Biotechnology 19, no. 3 (2021): 539–45. http://dx.doi.org/10.15625/1811-4989/13897.

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DNA marker is commonly used to determine the size of DNA fragments by electrophoresis in routine molecular biology laboratories. In this study, we report a new procedure to prepare recombinant plasmids pSY-60 which was partially digested by one restriction enzyme for generating DNA markers of 7 fragments from 60 to 420 bp. The procedure included a synthesis of 60 bp DNA fragment with EcoRI sites at both ends using PCR extension, self-ligation of the 60 bp fragments and subcloning the ligated product into plasmid, generating recombinant pSY-60. Once being cloned, 500 ng of 420 bp fragment purif
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Mann, C., and R. W. Davis. "Structure and sequence of the centromeric DNA of chromosome 4 in Saccharomyces cerevisiae." Molecular and Cellular Biology 6, no. 1 (1986): 241–45. http://dx.doi.org/10.1128/mcb.6.1.241-245.1986.

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The CEN4 sequences from chromosome 4 that impart mitotic stability to autonomously replicating (ARS) plasmids in yeast cells have been localized to a 1,755-base-pair (bp) fragment. This fragment could be cut in half to give two adjacent, nonoverlapping fragments, that each contained some mitotic stabilization sequences. One of the half-fragments worked as efficiently as the larger fragment from which it was derived, while the other half provided a much poorer degree of mitotic stabilization. Sequencing of 2,095 bp of DNA including this region revealed the presence of a centromere consensus seq
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Mann, C., and R. W. Davis. "Structure and sequence of the centromeric DNA of chromosome 4 in Saccharomyces cerevisiae." Molecular and Cellular Biology 6, no. 1 (1986): 241–45. http://dx.doi.org/10.1128/mcb.6.1.241.

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The CEN4 sequences from chromosome 4 that impart mitotic stability to autonomously replicating (ARS) plasmids in yeast cells have been localized to a 1,755-base-pair (bp) fragment. This fragment could be cut in half to give two adjacent, nonoverlapping fragments, that each contained some mitotic stabilization sequences. One of the half-fragments worked as efficiently as the larger fragment from which it was derived, while the other half provided a much poorer degree of mitotic stabilization. Sequencing of 2,095 bp of DNA including this region revealed the presence of a centromere consensus seq
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WU, Z., I. NAGANO, E. POZIO, and Y. TAKAHASHI. "Polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) for the identification of Trichinella isolates." Parasitology 118, no. 2 (1999): 211–18. http://dx.doi.org/10.1017/s0031182098003679.

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In the present study, polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) analysis was developed to identify 5 species (Trichinella spiralis, Trichinella britovi, Trichinella nativa, Trichinella nelsoni and Trichinella pseudospiralis) and 3 phenotypes of uncertain taxonomic status (Trichinella T5, T6, and T8). Eleven restriction endonucleases were used to restrict 3 DNA fragments (1) a 2800 bp fragment of the 43 kDa excretory–secretory (E–S) protein gene, (2) a 1250 bp fragment amplified with the primer pair SB147A and (3) a 372 bp fragment amplified with the primer p
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Huang, Yanqin, Jiayi Mu, Lina Qi, et al. "Diverse fragment lengths dismiss size selection for serum cell-free DNA: a comparative study of serum and plasma samples." Clinical Chemistry and Laboratory Medicine (CCLM) 58, no. 9 (2020): 1451–59. http://dx.doi.org/10.1515/cclm-2020-0078.

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AbstractBackgroundThe objective of this study was to determine the features of fragment length for circulating cell-free DNA (cfDNA) from plasma and serum samples.MethodsPlasma and serum samples from different sources were randomly collected. Circulating cfDNA was extracted and purified by a precipitation-enriched and spin-column-based kit. The concentration of the purified DNA was immediately measured by a highly sensitive dsDNA quantitative assay, and then the fragment length was analyzed by capillary electrophoresis. The abundance of a specific fragment was estimated by the area under curve
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Farrow, S. M., N. S. Hawa, R. Karmali, M. Hewison, J. C. Walters, and J. L. H. O'Riordan. "Binding of the receptor for 1,25-dihydroxyvitamin D3 to the 5′-flanking region of the bovine parathyroid hormone gene." Journal of Endocrinology 126, no. 3 (1990): 355—NP. http://dx.doi.org/10.1677/joe.0.1260355.

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ABSTRACT Receptors for 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) were prepared from bovine parathyroid glands and incubated with fragments of DNA of the 5′-flanking region of the bovine parathyroid hormone (PTH) gene covering 1700 base pairs (bp) upstream of the initiation site. In filter binding assays, incubation of the DNA fragment spanning − 700 to + 50 bp with 200 μg cytosolic protein gave 288±63% (mean ± s.d.) of binding in the absence of protein. In contrast, there was no significant reaction with the −1350 to − 700 bp fragment, nor was there binding of the receptor to a fragment of DNA c
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Stein, Daniel C. "Transformation of Neisseria gonorrhoeae: physical requirements of the transforming DNA." Canadian Journal of Microbiology 37, no. 5 (1991): 345–49. http://dx.doi.org/10.1139/m91-056.

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The 1600-bp (base pair) fragment encoding a portion of the nalidixic acid resistant DNA gyrase, subunit B, was characterized to determine what parameters effect transformation in the gonococcus. When this DNA (pSY2) was isolated from Escherichia coli, it was able to transform a variety of gonococcal strains to resistance to nalidixic acid via DNA-mediated transformation, irrespective of their restriction–modification phenotype. Nalidixic acid resistant transformants contained no plasmid DNA sequences that corresponded to the vector, as measured by plasmid screening procedures and colony hybrid
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BROWN, Philip M., and Keith R. FOX. "Nucleosome core particles inhibit DNA triple helix formation." Biochemical Journal 319, no. 2 (1996): 607–11. http://dx.doi.org/10.1042/bj3190607.

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We have used DNase I footprinting to examine the formation of DNA triple helices at target sites on DNA fragments that have been reconstituted with nucleosome core particles. We show that a 12 bp homopurine target site, located 45 bp from the end of the 160 bp tyrT(46A) fragment, cannot be targeted with either parallel (CT-containing) or antiparallel (GT-containing) triplex-forming oligonucleotides when reconstituted on to nucleosome core particles. Binding is not facilitated by the presence of a triplex-binding ligand. However, both parallel and antiparallel triplexes could be formed on a tru
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Haab, Brian B., and Richard A. Mathies. "Optimization of Single-Molecule Fluorescence Burst Detection of ds-DNA: Application to Capillary Electrophoresis Separations of 100–1000 Basepair Fragments." Applied Spectroscopy 51, no. 10 (1997): 1579–84. http://dx.doi.org/10.1366/0003702971939136.

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Methods for optimizing the dye labeling, laser excitation, and data analysis for single-molecule fluorescence burst detection of ds-DNA have been developed and then validated through capillary electrophoresis (CE) separations of 100–1000 basepair (bp) DNA. Confocal microscopy is used to observe fluorescence bursts from individual DNA fragments labeled with the intercalation dye TO6 as they pass through the ∼ 2-μm-diameter focused laser beam. The dye concentration and laser power were optimized by studying fluorescence burst intensities from pBluescript DNA fragments. The optimal TO6 concentrat
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Liu, Dong, Alyson Mack, Rongchen Wang, et al. "Functional Dissection of the cis-Acting Sequences of the Arabidopsis Transposable Element Tag1 Reveals Dissimilar Subterminal Sequence and Minimal Spacing Requirements for Transposition." Genetics 157, no. 2 (2001): 817–30. http://dx.doi.org/10.1093/genetics/157.2.817.

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Abstract The Arabidopsis transposon Tag1 has an unusual subterminal structure containing four sets of dissimilar repeats: one set near the 5′ end and three near the 3′ end. To determine sequence requirements for efficient and regulated transposition, deletion derivatives of Tag1 were tested in Arabidopsis plants. These tests showed that a 98-bp 5′ fragment containing the 22-bp inverted repeat and four copies of the AAACCX (X = C, A, G) 5′ subterminal repeat is sufficient for transposition while a 52-bp 5′ fragment containing only one copy of the subterminal repeat is not. At the 3′ end, a 109-
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Części książek na temat "Bp DNA fragment"

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"Introduction." In DNA Fingerprinting, edited by Lorne t. Kirby. Oxford University Press, 1993. http://dx.doi.org/10.1093/oso/9780716770015.003.0004.

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DNA identification analysis, identity testing, profiling, fingerprinting, typing, or genotyping refers to the characterization of one or more relatively rare features of an individuals’s genome or hereditary makeup. Every human, lower animal, and sexually reproduced plant has a characteristic phenotype or physical appearance because each possesses a unique hereditary composition. The exception to this rule is identical twins, who possess the same unique genotype but, owing to the consequences of complex developmental events, have subtly different phenotypes. The DNA of any individual is identi
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Niessen, M. L., and R. F. Vogel. "Monitoring of trichothecene producing Fusarium species in brewing cereals using a group specific Polymerase Chain Reaction." In European Brewery Convention. Oxford University PressOxford, 1997. http://dx.doi.org/10.1093/oso/9780199636907.003.0007.

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Abstract A pair of PCR primers (Tox5-1 and Tox5-2) was designed and a PCR set up and optimized. With the primer pair used a 658 bp fragment was amplified from DNA isolated from Fusarium species described as producers of trichothecene mycotoxins. Non-trichothecene producers, cereals, and bacteria gave no signal in the assay. Trichothecene producers were detected in samples of contaminated malts. Results showed that the method developed is group specific for the detection of all Fusarium species capable of producing trichothecenes. The method may be used for quality monitoring in the brewing and
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Leiβner, Christian E. W., Martin L. Niessen, and Rudi F. Vogel. "Nachweis von Fusarium graminearum und Fusarium culmorum unter Verwendung einer sektionsspezifischen Polymerase-kettenreaktion." In European Brewery Convention. Oxford University PressOxford, 1997. http://dx.doi.org/10.1093/oso/9780199636907.003.0008.

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Abstract Fusarium graminearum, F. culmorum, F. avenaceum, and Microdochium nivale are the most serious funga.l contaminants found in cereal grain. F. graminearum and F. culmorum may produce mycotoxins and have been found to be closely linked to the induction of gushing in beer. A pair of PCR primers was designed to amplify a 288 bp fragment from the DNA isolated from fusaria of the Discolor section. This PCR selectively detects F. graminearum and F. culmorum in sample material without being sensitive to other organisms present. The method described can be used in quality control in breweries a
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Guichard, Annabel, Evelyne Bergeret, and Ruth Griffin-Shea. "Rotund Rac-GAP." In Guidebook to the Sinall GTPases. Oxford University PressOxford, 1995. http://dx.doi.org/10.1093/oso/9780198599456.003.0077.

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Abstract The RnRac-GAP coding sequence, originally named pc1.7 and now renamed rnRac-GAP(l), was isolated (Agnel et al. 1989) by screening a cDNA library in Xgt10 prepared from pupae 5-7.5 days after egg laying (Poole etal. 1985) with a genomic fragment from the cloned rn region. Comparison of rnRac-GAP(l) with the corresponding genomic DNA showed the presence of a 482-bp intron at the 5’-end. Sequence and restriction map analysis of the insert of a second cDNA clone, originally named pc1.7d and now renamed rnRac-GAP(2), showed that the two inserts differed only at their 3’-ends.
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"Red Snapper: Ecology and Fisheries in the U.S. Gulf of Mexico." In Red Snapper: Ecology and Fisheries in the U.S. Gulf of Mexico, edited by JOHN R. GOLD and ERIC SAILLANT. American Fisheries Society, 2007. http://dx.doi.org/10.47886/9781888569971.ch13.

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<em>Abstract</em>.—Allelic variation at 19 nuclear-encoded microsatellite loci and haplotype variation in a 590 bp protein-coding fragment of mitochondrial (mt)DNA were assayed among Gulf red snapper sampled from four cohorts at each of three offshore localities (12 samples total) in the northern Gulf of Mexico. Significant heterogeneity in allele and genotype distributions among samples was detected at four microsatellites; six of seven ‘significant’ pairwise comparisons between samples revealed the heterogeneity to be temporal rather than spatial. Nested-clade analysis of mtDNA v
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Wuster, W., G. M. Salomao, R. S. Thorpe, et al. "Systematics of the Bothrops atrox complex: new insights from multivariate analysis and mitochondrial DNA sequence information." In Venomous Snakes. Oxford University PressOxford, 1996. http://dx.doi.org/10.1093/oso/9780198549864.003.0008.

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Abstract We use multivariate analysis of morphological characters and comparative sequencing of a 520 bp fragment of the cytochrome b gene (mitochondrial DNA) to investigate patterns of geographic variation in the Bothrops atrox species complex, and the population phylogeny of the group in parts of South America. Populations conventionally assigned to B. atrox and B. moojeni constitute morphologically distinct groupings, with a zone of phenetically intermediate specimens where their ranges meet. Bothrops marajoensis, B. isabelae, B. leucurus and B. pradoi are poorly differentiated or undiffere
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Towner, Paul. "Recovery of DNA from electrophoresis gels." In Essential Molecular Biology. Oxford University PressOxford, 2000. http://dx.doi.org/10.1093/oso/9780199636426.003.0006.

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Abstract During molecular biology projects it is frequently necessary to isolate DNA fragments for further manipulations. The DNA could be from many sources but would typically be from PCRs or restriction enzyme digestion of recombinant plasmids. The simplest way to separate DNA is by agarose gel electrophoresis in the presence of ethidium bromide, the required DNA bands being identified using a UV transilluminator. The quality of agarose is now so good that concentrations as high as 8% (w/v) can be cast and used to separate DNA fragments down to 30 bp with very good resolution, allowing virtu
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Yoshida, Akira, Rong-Guang Shao, and Yves Pommier. "Assessment of DNA damage in apoptosis." In Apoptosis. Oxford University PressOxford, 1999. http://dx.doi.org/10.1093/oso/9780199637843.003.0003.

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Abstract Apoptosis is morphologically characterized by early cell shrinkage, chromatin condensation, nuclear fragmentation, cell surface blebbing, and membrane bound apoptotic bodies (1) and these changes are described in Chapter 2. One of the most extensively studied biochemical events in apoptosis is chromatin fragmentation by endonuclease activation. DNA double-strand cleavage occurs in the linker regions between nucleosomes, and produces DNA fragments that are multiples of approximately 185 base pairs (bp) (2-4). These fragments can readily be demonstrated by agarose gel electrophoresis as
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Luijt, Rob B. Van Der, Riccardo Fodde,, and Johan T. Den Dunnen. "The protein truncation test (PTT)." In Mutation Detection. Oxford University PressOxford, 1998. http://dx.doi.org/10.1093/oso/9780199636570.003.0012.

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Abstract The detection and characterization of point mutations in genes responsible for inherited disorders has become one of the most common practices in human molecular genetics. Several protocols are nowadays available: single strand conformation polymorphism (SSCP) (1), RNase protection (2), hydroxylamine and osmium tetroxide (HOT) chemical cleavage (3), and denaturing gradient gel electrophoresis (DGGE) (4, 5). These techniques are generally aimed at the identification of single base substitutions, insertions, or deletions in relatively small DNA fragments (50-500 bp). However, when deali
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"DMA Amplification." In DNA Fingerprinting, edited by Lorne t. Kirby. Oxford University Press, 1993. http://dx.doi.org/10.1093/oso/9780716770015.003.0008.

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Amplification of DNA may be necessary to increase the quantity of sample available for profiling, to reduce the analysis time, or to produce probes for the hybridization process (Higuchi 1989, Li 1988, Marx 1988, Mullis 1990, Paabo 1989, Saiki 1986). Stretches of nucleotides up to at least 3,000 bp from any DNA-containing samples may be efficiently amplified by the polymerase chain reaction (PCR). Alternatively, living tissue can be placed in culture, and fibroblasts, epithelial type cells, or lymphoblasts grown. The culture process differs considerably from the PCR approach in that the total
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Streszczenia konferencji na temat "Bp DNA fragment"

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Chen, P. C., D. S. Park, B. H. You, et al. "A High Throughput Microfluidic Thermal Reactor." In ASME 2009 International Mechanical Engineering Congress and Exposition. ASMEDC, 2009. http://dx.doi.org/10.1115/imece2009-13130.

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A high throughput microfluidic system including a 96 continuous flow (CF) thermal reactors and a multi-zone thermal control system was designed and fabricated. An infrared camera (IR) was used to analyze and verify the uniformity of the temperature distribution. Temperature variations from the nominal values were ±2°C in the denaturation zone and ±1°C in the renaturation and extension zones. Six different DNA fragments, with lengths ranging from 99 bp to 997 bp, were obtained from a λ-DNA template, each with a distinct renaturation temperature. As an initial demonstration of the biochemical pe
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Nishino, M., T. Nishimura, H. Naka, S. Mikami, A. Yoshioka, and H. Fukui. "CARRIER DETECTION IN JAPANESE FAMILIES WITH HAEMOPHILIA A USING FACTOR VIII GENE PROBE(F8A) AND ST 14-1 PROBE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644009.

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Recently, the gene structure for human F.VIII protein was clarified, and F.VIII DNA probes have been used for carrier detection and prenatal diagnosis ofhaemophilia A. In order to make sure that the phenomena are universal, we have analysed the RFLPs of F.VIII gene in 16 Japanese families with haemophilia A, including a female haemophiliac case, using an intragenic F.VIII DNA probe(F8A) and an extragenic(linked) DNA probe(Stl4-1).The probe F8A revealed two variant bands after digestion by Bel I. Of normal 60 X chromosomes (females) examined, about 85% bore the 879-bp fragment and 15%the 1165-b
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Daly, John, and Mark Davies. "A Quantitative Free Convection DNA Amplifier." In ASME/JSME 2007 Thermal Engineering Heat Transfer Summer Conference collocated with the ASME 2007 InterPACK Conference. ASMEDC, 2007. http://dx.doi.org/10.1115/ht2007-32381.

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The Polymerase Chain Reaction (PCR) has been used extensively to amplify targeted nucleic acids for many applications in molecular biology and, increasingly, in medical diagnostics. Outlined in this paper is a PCR device which takes account of the advantages offered by free convection. The design is, in it fundamental format a time-wise isothermal well-based thermocycler. A temperature gradient induced across the well causes convection forces to circulate the sample through the required temperatures necessary for amplification. Quantitative amplification is demonstrated with real time measurem
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Chelucci, C., H. J. Hassan, R. Guerriero, A. Leonardi, G. Mattia, and C. Peschle. "POLYMORPHIC SITES IN FACTOR X GENE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643836.

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The structure of factor X gene has been analyzed by Southern blot in 5 subjects with factor X deficiency.Genomic DNA was digested with 8 different endonucleases and hybridized with a cDNA probe. The congenital deficiency observed in these patients is not apparently due to a major deletion or rearrangement. Since the gene locus is grossly intact, the disease presumably results from point mutation(s) not identified by the utilized endonucleases.Our study was also focused on the presence of polymorphic site(s) in the factor X gene locus. Analysis of 50 normal subjects allowed to identify several
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Daly, John, and Mark Davies. "A Natural Convection DNA Amplifier." In ASME 4th International Conference on Nanochannels, Microchannels, and Minichannels. ASMEDC, 2006. http://dx.doi.org/10.1115/icnmm2006-96244.

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Natural convection is the driver of innumerable natural world phenomena. Within the laboratory, it offers simplified geometries and flow structures without the need for auxiliary flow inducement, thereby greatly reducing the risk of external contamination within biomedical applications. Outlined in this paper is a polymerase chain reaction (PCR) device which takes advantage of these distinct qualities. PCR has become synonymous with DNA amplification in molecular biology laboratories throughout the world, and at the heart of PCR is thermal cycling. Commonly PCR is accomplished utilising a thre
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G. V., Izotova, Vlasenko P. G., Kashinskaya E. N., and Solovyev M. M. "IDENTIFICATION OF DIPLOSTOMOSIS PATHOGENS OF PREDOMINATING FISH SPECIES OF SIBERIA USING DNA-BARCODING APPROACH." In II INTERNATIONAL SCIENTIFIC AND PRACTICAL CONFERENCE "DEVELOPMENT AND MODERN PROBLEMS OF AQUACULTURE" ("AQUACULTURE 2022" CONFERENCE). DSTU-Print, 2022. http://dx.doi.org/10.23947/aquaculture.2022.53-55.

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Metacercariae of the genus Diplostomum are localized in the eyes and the brain of freshwater fish and cyclostomata species causing different types of diplostomosis. Hyperinvasion by these parasites lead to spatial disorientation, changing in feeding activity, lowering the growth rate, etc. Species identification of Diplostomum spp. based on the morphological features is difficult due to the lack and severity of them. In present study, species diversity of the genus Diplostomum metacercariae parasitizing in fishes of Chany, Baunt and Teletskoye lakes is defined according to the DNA-barcoding ap
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R. Abduwahhab ALHEANY, Aya. "GENOTYPING AND SEROPREVALENCE OF TOXOPLASMA GONDII IN ABORTION WITH ELEVATION OF INTERLEUKIN-6 LEVEL." In IV.International Scientific Congress of Pure,Appliedand Technological Sciences. Rimar Academy, 2022. http://dx.doi.org/10.47832/minarcongress4-12.

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Toxoplasmosis is a harmful microorganism that resides inside the cells of the host and produces serious symptoms such as abortion. A total of (48) blood samples were taken from aborted women in this study, with (40) healthy persons serving as a control group. Between the 15th of January and the 30th of September 2021, the patients were treated at Al-Karkh Maternity Hospital. The infection rate was highest in the age range (18-25) years for both the infected women and the control group, according to the findings. Among aborted mothers with Toxoplasmosis, the IgG antibody positivity rate was 40
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Bagdat, A., D. Ziyabek, Zh Muslimova, and E. Usenbekov. "DIAGNOSTICS OF CARRIERS OF HH2 FERTILITY HAPLOTYPES IN HOLSTIN COWS." In SCIENTIFIC SUPPORT FOR LIVESTOCK BREEDING IN SIBERIA. Krasnoyarsk Scientific Research Institute of Agriculture is a separate division of the Federal Research Center KSC SB RAS, 2024. https://doi.org/10.52686/conferencearticle_67597ceef0f045.18547197.

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The authors of the article used the Tetra-Primer ARMS-PCR reaction method to identify heterozygous carriers of the HH2 fertility haplotype in Holstein cows of foreign selection. A total of 150 DNA samples were tested, of which 8 individuals turned out to be heterozygous carriers of single-nucleotide deletion; the frequency of the harmful mutation was 5.4%. It has been established that the detection of DNA fragments on an electropherogram with sizes of 281 bp, 184 bp. and 145 bp, indicates that the animal is a heterozygous carrier of the HH2 fertility haplotype. It should be noted that to contr
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Huber, P., J. Dalmon, M. Laurent, G. Courtois, D. Thevenon та G. Marguerie. "CHARACTERIZATION OFTHE 5’FLANKING REGION FOR THE HUMAN FIBRINOGEN β GENE". У XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642889.

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Fibrinogen is coded by three separate genes located in a 50kb region of chromosome 4 and organized in a α - β - γ orientation with an inversion of the gene 3- A human genomic library was constructed using the EMBL4 phage and screened with cDNA probes coding for human fibrinogen Aα, Bβ and γ chains. Clones, covering the fibrinogen locus,were identified, and their organization was analyzed by means of hybridization and restriction mapping. Among these clones one recombinant phage containing the β gene and large 5’ and 3’ -flanking sequences was isolated.To identify the regulatory sequences Dpstr
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Chen, Pin-Chuan, Jifeng Chen, Dimitris E. Nikitopoulos, Steven A. Soper, and Michael C. Murphy. "Performance of an Electrokinetic Shuttle Polymerase Chain Reactor." In ASME 2006 International Mechanical Engineering Congress and Exposition. ASMEDC, 2006. http://dx.doi.org/10.1115/imece2006-15239.

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An alternative polymerase chain reactor (PCR) driven by electrokinetic flow was developed and tested. A single straight microchannel and a double-T intersection were designed for injection of DNA samples and thermal cycling by shuttling between constant temperature zones. Thermal performance of the device was studied using numerical and analytical models to understand the temperature distribution. Devices were made on a polycarbonate substrate by hot embossing with a micromilled brass mold insert. A PID control system, with a tolerance of ± 0.2°C, was used to maintain the temperatures in each
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Do bibliografii