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Artykuły w czasopismach na temat „Carboxylic acids Metabolism”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 1 lutego 2022

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1

Iwami, Y., S. Hata, N. Takahashi, and T. Yamada. "Difference in Amounts between Titratable Acid and Total Carboxylic Acids Produced by Oral Streptococci during Sugar Metabolism." Journal of Dental Research 68, no. 1 (January 1989): 16–19. http://dx.doi.org/10.1177/00220345890680010101.

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The acid produced by the resting cells of Streptococcus mutans NCTC 10449 and HS 6 and S. sanguis ATCC 10556 during sugar metabolism was estimated with a pH-stat and a carboxylic acid analyzer. Lactic, formic, acetic, pyruvic, and carbonic acids were detected in the reaction mixtures, but propionic, citric, succinic, iso-butyric, butyric, iso-valeric, and valeric acids were not detected. The amount of titratable acid estimated by alkaline titration with the pH-stat was larger than the amount of total carboxylic acids estimated with the carboxylic acid analyzer. The difference in quantity betwe

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2

Goyal, R., R. Tardif, and J. Brodeur. "Influence of a cysteine prodrug, L-2-oxothiazolidine-4-carboxylic acid, on the urinary elimination of mercapturic acids of ethylene oxide, dibromoethane, and acrylonitrile: a dose–effect study." Canadian Journal of Physiology and Pharmacology 67, no. 3 (March 1, 1989): 207–12. http://dx.doi.org/10.1139/y89-035.

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Metabolic disposition of ethylene oxide, dibromoethane, and acrylonitrile in rats after acute exposure was studied by examining the relationship between dose and urinary metabolites, and by establishing the influence of a glutathione precursor, L-2-oxothiazolidine-4-carboxylic acid (OTCA), on the above relationship. Respective urinary metabolites, hydroxyethylmercapturic acid, cyanoethylmercapturic acid, thiocyanate, and ethylene glycol, were quantified to estimate the extent to which each compound was metabolized. The animals were given either ethylene oxide (0.34, 0.68, or 1.36 mmol/kg), dib

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Sarkar, Omprakash, A. Naresh Kumar, Shikha Dahiya, K. Vamshi Krishna, Dileep Kumar Yeruva, and S. Venkata Mohan. "Regulation of acidogenic metabolism towards enhanced short chain fatty acid biosynthesis from waste: metagenomic profiling." RSC Advances 6, no. 22 (2016): 18641–53. http://dx.doi.org/10.1039/c5ra24254a.

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Darnell, Malin, and Lars Weidolf. "Metabolism of Xenobiotic Carboxylic Acids: Focus on Coenzyme A Conjugation, Reactivity, and Interference with Lipid Metabolism." Chemical Research in Toxicology 26, no. 8 (July 5, 2013): 1139–55. http://dx.doi.org/10.1021/tx400183y.

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Beaulieu, Pierre L., René Coulombe, James Gillard, Christian Brochu, Jianmin Duan, Michel Garneau, Eric Jolicoeur, et al. "Allosteric N-acetamide-indole-6-carboxylic acid thumb pocket 1 inhibitors of hepatitis C virus NS5B polymerase — Acylsulfonamides and acylsulfamides as carboxylic acid replacements." Canadian Journal of Chemistry 91, no. 1 (January 2013): 66–81. http://dx.doi.org/10.1139/cjc-2012-0319.

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Acylsulfonamide and acylsulfamide as surrogates for the carboxylic acid function of N-acetamide-indole-6-carboxylic acids were evaluated as allosteric inhibitors of hepatitis C virus (HCV) NS5B polymerase. Several analogs displayed excellent antiviral potency against both 1a and 1b HCV genotypes in cell-based subgenomic replicon assays. Structure–activity relationships (SAR) are discussed in the context of the crystal structure of an inhibitor − NS5B polymerase complex. Absorption, distribution, metabolism, and excretion pharmacokinetic (ADME-PK) properties of this class of inhibitors are also

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6

Omran, Arthur, Cesar Menor-Salvan, Greg Springsteen, and Matthew Pasek. "The Messy Alkaline Formose Reaction and Its Link to Metabolism." Life 10, no. 8 (July 28, 2020): 125. http://dx.doi.org/10.3390/life10080125.

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Sugars are essential for the formation of genetic elements such as RNA and as an energy/food source. Thus, the formose reaction, which autocatalytically generates a multitude of sugars from formaldehyde, has been viewed as a potentially important prebiotic source of biomolecules at the origins of life. When analyzing our formose solutions we find that many of the chemical species are simple carboxylic acids, including α-hydroxy acids, associated with metabolism. In this work we posit that the study of the formose reaction, under alkaline conditions and moderate hydrothermal temperatures, shoul

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Knights, Kathleen M., Matthew J. Sykes, and John O. Miners. "Amino acid conjugation: contribution to the metabolism and toxicity of xenobiotic carboxylic acids." Expert Opinion on Drug Metabolism & Toxicology 3, no. 2 (April 2007): 159–68. http://dx.doi.org/10.1517/17425255.3.2.159.

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Bock, Susanne, Ulrich A. Sedlmeier, and Klaus H. Hoffmann. "Metabolism of absorbed short-chain carboxylic acids by the freshwater oligochaete Tubifex tubifex." Comparative Biochemistry and Physiology Part B: Comparative Biochemistry 92, no. 1 (January 1989): 35–40. http://dx.doi.org/10.1016/0305-0491(89)90309-x.

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9

Knights, Kathleen M. "ROLE OF HEPATIC FATTY ACID:COENZYME A LIGASES IN THE METABOLISM OF XENOBIOTIC CARBOXYLIC ACIDS." Clinical and Experimental Pharmacology and Physiology 25, no. 10 (October 1998): 776–82. http://dx.doi.org/10.1111/j.1440-1681.1998.tb02152.x.

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10

Arun, Viswanath, Takashi Mino, and Tomonori Matsuo. "Metabolism of Carboxylic Acids Located in and around the Glycolytic Pathway and the TCA Cycle in the Biological Phosphorus Removal Process." Water Science and Technology 21, no. 4-5 (April 1, 1989): 363–74. http://dx.doi.org/10.2166/wst.1989.0238.

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Anaerobic batch experiments were performed to measure the profile of various parameters when phosphorus accumulating sludge acclimatized under anaerobic-aerobic operation is fed with malic, lactic, pyruvic, propionic and succinic acids - carboxylic acids that lie in the vicinity of the Glycolytic pathway and the Tricarboxylic acid cycle - two pathways central to the utilization of organics as sources of energy and carbon in most microorganisms. Carbon dioxide gas produced during substrate uptake was also measured. In most cases, intra-cellular carbohydrate reserves are consumed and the NADH2 t

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11

Di Carlo, Frederick J. "Structure-Activity Relationships (Sar) and Structure-Metabolism Relationships (Smr) Affecting the Teratogenicity of Carboxylic Acids." Drug Metabolism Reviews 22, no. 5 (January 1990): 411–49. http://dx.doi.org/10.3109/03602539008991446.

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12

Daneshvar, Maryam I., John B. Brooks, Georgia B. Malcolm, and Leo Pine. "Analyses of fermentation products of Listeria species by frequency-pulsed electron-capture gas–liquid chromatography." Canadian Journal of Microbiology 35, no. 8 (August 1, 1989): 786–93. http://dx.doi.org/10.1139/m89-131.

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Aerobic fermentation broths of eight Listeria monocytogenes strains, two or more strains of the remaining six Listeria species, and one strain of Jonesia denitrificans were examined by frequency-pulsed electron-capture gas–liquid chromatography for carboxylic acids, alcohols, amines, and hydroxy acids. All species produced acetic, isobutyric, butyric, isovaleric, phenylacetic, lactic, 2-hydroxybutyric, 2-hydroxyvaleric, and 2-hydroxyisocaproic acids. Propionic acid was not formed, and traces of isocaproic acid were observed. Of the alcohol and amine derivatives observed, only acetylmethylcarbi

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13

Leitão, Ana Lúcia, and Francisco J. Enguita. "Structural Insights into Carboxylic Polyester-Degrading Enzymes and Their Functional Depolymerizing Neighbors." International Journal of Molecular Sciences 22, no. 5 (February 26, 2021): 2332. http://dx.doi.org/10.3390/ijms22052332.

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Esters are organic compounds widely represented in cellular structures and metabolism, originated by the condensation of organic acids and alcohols. Esterification reactions are also used by chemical industries for the production of synthetic plastic polymers. Polyester plastics are an increasing source of environmental pollution due to their intrinsic stability and limited recycling efforts. Bioremediation of polyesters based on the use of specific microbial enzymes is an interesting alternative to the current methods for the valorization of used plastics. Microbial esterases are promising ca

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14

Van Dyk, Tina K., Lori J. Templeton, Keith A. Cantera, Pamela L. Sharpe, and F. Sima Sariaslani. "Characterization of the Escherichia coli AaeAB Efflux Pump: a Metabolic Relief Valve?" Journal of Bacteriology 186, no. 21 (November 1, 2004): 7196–204. http://dx.doi.org/10.1128/jb.186.21.7196-7204.2004.

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ABSTRACT Treatment of Escherichia coli with p-hydroxybenzoic acid (pHBA) resulted in upregulation of yhcP, encoding a protein of the putative efflux protein family. Also upregulated were the adjacent genes yhcQ, encoding a protein of the membrane fusion protein family, and yhcR, encoding a small protein without a known or suggested function. The function of the upstream, divergently transcribed gene yhcS, encoding a regulatory protein of the LysR family, in regulating expression of yhcRQP was shown. Furthermore, it was demonstrated that several aromatic carboxylic acid compounds serve as induc

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15

She, Jianqing, Manyun Guo, Hongbing Li, Junhui Liu, Xiao Liang, Peining Liu, Bo Zhou, et al. "Targeting amino acids metabolic profile to identify novel metabolic characteristics in atrial fibrillation." Clinical Science 132, no. 19 (October 5, 2018): 2135–46. http://dx.doi.org/10.1042/cs20180247.

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Background: Atrial fibrillation (AF) is the most common cardiac arrhythmia whose incidence is on the rise globally. However, the pathophysiologic mechanism of AF remains poorly understood and there has been a lack of circulatory markers to diagnose and predict prognosis of AF. In the present study, by measuring metabolic profile and analyzing plasma amino acid levels in AF patients, we sought to determine whether amino acid metabolism was correlated to the occurrence of AF. Methods: Consecutive patients admitted to hospital for AF were enrolled. Plasma samples were obtained after overnight fas

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16

Covington, D. K., C. A. Briscoe, A. J. Brown, and C. K. Jayawickreme. "The G-protein-coupled receptor 40 family (GPR40–GPR43) and its role in nutrient sensing." Biochemical Society Transactions 34, no. 5 (October 1, 2006): 770–73. http://dx.doi.org/10.1042/bst0340770.

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Recent deorphanization efforts have paired the G-protein-coupled receptors GPR40, GPR41 and GPR43 with fatty acids as endogenous ligands. While carboxylic acids have been historically known to serve as fuel sources and biomarkers of disease, these studies demonstrate that fatty acids can act as signalling molecules at the cell-surface level. This receptor subfamily shares approx. 30% identity among members, with some limited cross-over between ligand activities. Generalized expression patterns within the pancreatic β-cell, adipose depots and the gastrointestinal tract infer involvement in ener

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17

Floret, Fabienne, Lal C. Chaudhary, William C. Ellis, Suliman El Hassan, Nest McKain, C. James Newbold, and R. John Wallace. "Influence of 1-[(E)-2-(2-Methyl-4-Nitrophenyl)Diaz-1-enyl]Pyrrolidine-2-Carboxylic Acid and Diphenyliodonium Chloride on Ruminal Protein Metabolism and Ruminal Microorganisms." Applied and Environmental Microbiology 65, no. 7 (July 1, 1999): 3258–60. http://dx.doi.org/10.1128/aem.65.7.3258-3260.1999.

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ABSTRACT The effects of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine-2-carboxylic acid (LY29) and diphenyliodonium chloride (DIC) on the degradation of protein to ammonia were determined in a mixed rumen microbial population taken from sheep on a grass hay-concentrate diet. Both compounds decreased NH3 production by inhibiting deamination of amino acids. LY29, but not DIC, inhibited growth of the high-activity ammonia-producing species, Clostridium aminophilum and Clostridium sticklandii.

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Komuro, Masakatsu, Tsunehiko Higuchi, and Masaaki Hirobe. "Application of chemical cytochrome P-450 model systems to studies on drug metabolism—VIII. Novel metabolism of carboxylic acids via oxidative decarboxylation." Bioorganic & Medicinal Chemistry 3, no. 1 (January 1995): 55–65. http://dx.doi.org/10.1016/0968-0896(94)00141-o.

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Wojakowska, Anna, Monika Pietrowska, Piotr Widlak, Dariusz Dobrowolski, Edward Wylęgała, and Dorota Tarnawska. "Metabolomic Signature Discriminates Normal Human Cornea from Keratoconus—A Pilot GC/MS Study." Molecules 25, no. 12 (June 25, 2020): 2933. http://dx.doi.org/10.3390/molecules25122933.

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The molecular etiology of keratoconus (KC), a pathological condition of the human cornea, remains unclear. The aim of this work was to perform profiling of metabolites and identification of features discriminating this pathology from the normal cornea. The combination of gas chromatography and mass spectrometry (GC/MS) techniques has been applied for profiling and identification of metabolites in corneal buttons from 6 healthy controls and 7 KC patients. An untargeted GC/MS-based approach allowed the detection of 377 compounds, including 46 identified unique metabolites, whose levels enabled t

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20

Ruprecht, J. J., and E. R. S. Kunji. "Structural Mechanism of Transport of Mitochondrial Carriers." Annual Review of Biochemistry 90, no. 1 (June 20, 2021): 535–58. http://dx.doi.org/10.1146/annurev-biochem-072820-020508.

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Members of the mitochondrial carrier family [solute carrier family 25 (SLC25)] transport nucleotides, amino acids, carboxylic acids, fatty acids, inorganic ions, and vitamins across the mitochondrial inner membrane. They are important for many cellular processes, such as oxidative phosphorylation of lipids and sugars, amino acid metabolism, macromolecular synthesis, ion homeostasis, cellular regulation, and differentiation. Here, we describe the functional elements of the transport mechanism of mitochondrial carriers, consisting of one central substrate-binding site and two gates with salt-bri

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López-Millán, Ana Flor, Fermín Morales, Yolanda Gogorcena, Anunciación Abadía, and Javier Abadía. "Iron resupply-mediated deactivation of Fe-deficiency stress responses in roots of sugar beet." Functional Plant Biology 28, no. 3 (2001): 171. http://dx.doi.org/10.1071/pp00105.

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Different root zones with or without increased Fe-reducing activities have been studied in Fe-deficient sugar beet (Beta vulgaris L.) plants after Fe resupply to the nutrient solution. The subapical regions of roots from Fe-deficient plants decreased by 19 and 88% their capacity to reduce ferric chelates after 24 and 96 h of Fe resupply, respectively. Iron resupply caused 52 and 96% decreases in phosphoenolpyruvate carboxylase activity in root extracts after 24 and 96 h, respectively, and also caused general decreases in other enzyme activities involved in carboxylic acid metabolism. The large

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Lewis, Dustin F., Rory L. Roten, Wesley J. Everman, Travis W. Gannon, Robert J. Richardson, and Fred H. Yelverton. "Absorption, Translocation, and Metabolism of Aminocyclopyrachlor in Tall Fescue (Lolium arundinaceum)." Weed Science 61, no. 3 (September 2013): 348–52. http://dx.doi.org/10.1614/ws-d-12-00189.1.

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Synthetic auxin herbicides are commonly used in forage, pasture, range, and turfgrass settings for dicotyledonous weed control. Aminocyclopyrachlor (AMCP) is a newly developed pyrimidine carboxylic acid with a chemical structure and mode of action similar to the pyridine carboxylic acids—aminopyralid, clopyralid, and picloram. Injury to sensitive dicotyledonous plants has been observed following exposure to monocotyledonous plant material previously treated with pyridine compounds. The absorption, translocation, and metabolism of AMCP has been documented in susceptible broadleaf weeds; however

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Syromolotov, Alexander V., Alexander A. Kimyashov, and Sergey V. Sukhorukov. "Decarboxylation 2'-dicarboxy-5-(methyl-5'-indolyl-3')- indolyl-3-acetic acid with use of salts of copper." Butlerov Communications 58, no. 4 (April 30, 2019): 58–61. http://dx.doi.org/10.37952/roi-jbc-01/19-58-4-58.

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In this article a decarboxylation method based on the use of quinoline with a copper salt is discussed. Decarboxylation is the elimination of CO2 from the carboxylic group of carboxylic acids or the carboxylate group of their salts. The process is used to produce a large number of organic compounds, such classes as alkanes, alkenes, alcohols, ketones, ethers and esters. Decarboxylation plays an important role in the metabolism of living organisms, namely in the decarboxylation of amino acids. From this we can conclude that the process is important to study. Decarboxylation proceeds in differen

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Merelli, B., S. Hamm, A. Carlin-Sinclair, and J. C. Cherton. "N-acylaziridines as potential proinsecticides of carboxylic acids☆Part VI. Direct HPLC monitoring of the metabolism in insect tissues." Journal of Chromatography B 826, no. 1-2 (November 5, 2005): 129–38. http://dx.doi.org/10.1016/j.jchromb.2005.07.037.

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O'Kane, Robyn L., and Richard A. Hawkins. "Na+-dependent transport of large neutral amino acids occurs at the abluminal membrane of the blood-brain barrier." American Journal of Physiology-Endocrinology and Metabolism 285, no. 6 (December 2003): E1167—E1173. http://dx.doi.org/10.1152/ajpendo.00193.2003.

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Several Na+-dependent carriers of amino acids exist on the abluminal membrane of the blood-brain barrier (BBB). These Na+-dependent carriers are in a position to transfer amino acids from the extracellular fluid of brain to the endothelial cells and thence to the circulation. To date, carriers have been found that may remove nonessential, nitrogen-rich, or acidic (excitatory) amino acids, all of which may be detrimental to brain function. We describe here Na+-dependent transport of large neutral amino acids across the abluminal membrane of the BBB that cannot be ascribed to currently known sys

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Curiao, Tânia, Emmanuela Marchi, Carlo Viti, Marco R. Oggioni, Fernando Baquero, José Luis Martinez, and Teresa M. Coque. "Polymorphic Variation in Susceptibility and Metabolism of Triclosan-Resistant Mutants of Escherichia coli and Klebsiella pneumoniae Clinical Strains Obtained after Exposure to Biocides and Antibiotics." Antimicrobial Agents and Chemotherapy 59, no. 6 (March 30, 2015): 3413–23. http://dx.doi.org/10.1128/aac.00187-15.

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ABSTRACTExposure to biocides may result in cross-resistance to other antimicrobials. Changes in biocide and antibiotic susceptibilities, metabolism, and fitness costs were studied here in biocide-selectedEscherichia coliandKlebsiella pneumoniaemutants.E. coliandK. pneumoniaemutants with various degrees of triclosan susceptibility were obtained after exposure to triclosan (TRI), benzalkonium chloride (BKC), chlorhexidine (CHX) or sodium hypochlorite (SHC), and ampicillin or ciprofloxacin. Alterations in antimicrobial susceptibility and metabolism in mutants were tested using Phenotype MicroArra

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Zarafu, Irina, Lilia Matei, Coralia Bleotu, Petre Ionita, Arnaud Tatibouët, Anca Păun, Ioana Nicolau, et al. "Synthesis, Characterization, and Biologic Activity of New Acyl Hydrazides and 1,3,4-Oxadiazole Derivatives." Molecules 25, no. 14 (July 21, 2020): 3308. http://dx.doi.org/10.3390/molecules25143308.

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Starting from isoniazid and carboxylic acids as precursors, thirteen new hydrazides and 1,3,4-oxadiazoles of 2-(4-substituted-phenoxymethyl)-benzoic acids were synthesized and characterized by appropriate means. Their biological properties were evaluated in terms of apoptosis, cell cycle blocking, and drug metabolism gene expression on HCT-8 and HT-29 cell lines. In vitro antimicrobial tests were performed by the microplate Alamar Blue assay for the anti-mycobacterial activities and an adapted agar disk diffusion technique for other non-tubercular bacterial strains. The best antibacterial acti

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Jackson, Dennis P., Douglas A. Gray, Vincent L. Morris, and Diane A. Cuppels. "Identification of a DNA region required for growth of Pseudomonas syringae pv. tomato on tomato plants." Canadian Journal of Microbiology 38, no. 9 (September 1, 1992): 883–90. http://dx.doi.org/10.1139/m92-144.

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The prototrophic Pseudomonas syringae pv. tomato mutant DC3481, which is the result of a single-site Tn5 insertion, cannot grow and cause disease on tomato plants and cannot use the major organic acids of tomato, i.e., citric, malic, succinic, and tartaric acids, as sole carbon sources. Although nonpathogenic, strain DC3481 can still induce a hypersensitive reaction in nonhost plants. We have identified a 30-kb fragment of P. syringae pv. tomato wild-type DNA that can complement this mutant. EcoRI fragments from this region were subcloned and individually subjected to functional complementatio

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Ramos-González, Marı́a-Isabel, Patricia Godoy, Miguel Alaminos, Arie Ben-Bassat, and Juan-Luis Ramos. "Physiological Characterization ofPseudomonas putida DOT-T1E Tolerance top-Hydroxybenzoate." Applied and Environmental Microbiology 67, no. 9 (September 1, 2001): 4338–41. http://dx.doi.org/10.1128/aem.67.9.4338-4341.2001.

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ABSTRACT Pseudomonas putida DOT-T1E was isolated as a toluene-tolerant strain. We show that it is also able to grow on high concentrations (up to 17 g/liter [123 mM]) ofp-hydroxybenzoate (4HBA). Tolerance to this aromatic carboxylic acid (up to 30 g/liter [217 mM]) is improved by preexposing the cells to low 4HBA concentrations; the adaptation process is caused by the substrate itself rather than by products resulting from its metabolism. The mechanisms of 4HBA tolerance seem to involve increased rigidity of the cell membrane as a result of a decrease in the cis/trans ratio of unsaturated fatt

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Lhuguenot, J. C., A. C. Macherey, and M. Paitry. "Quantitative structure-activity relationship (QSAR) for peroxisome proliferation induced by branched carboxylic acids issued from diester plasticizers metabolism in rats." Toxicology Letters 95 (July 1998): 87. http://dx.doi.org/10.1016/s0378-4274(98)80345-7.

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Chatzigianni, Martina, Konstantinos A. Aliferis, Georgia Ntatsi, and Dimitrios Savvas. "Effect of N Supply Level and N Source Ratio on Cichorium spinosum L. Metabolism." Agronomy 10, no. 7 (July 2, 2020): 952. http://dx.doi.org/10.3390/agronomy10070952.

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Cichorium spinosum L. is considered a health-promoting vegetable that has been recently introduced in cultivation, and thus information on the responses of its different ecotypes to N supply level and source is largely fragmented. To cover this gap of knowledge, seeds of two different local ecotypes of C. spinosum L. originating from a coastal and a montane habitat of the island of Crete were propagated, and the obtained seedlings were grown hydroponically. The supplied nutrient solution differed in the total-N level (4 or 16 mmol L−1) and N source (NH4-N/-N/total-N: 0.05, 0.25, or 0.50). The

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Chovanec, Peter, Michal Kaliňák, Tibor Liptaj, Naďa Pronayová, Tibor Jakubík, Daniela Hudecová, and Ľudovít Varečka. "Study ofTrichoderma viridemetabolism under conditions of the restriction of oxidative processes." Canadian Journal of Microbiology 51, no. 10 (September 1, 2005): 853–62. http://dx.doi.org/10.1139/w05-075.

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Trichoderma viride was capable of growth and conidiation in the presence of high concentrations of the uncoupler 3,3′,4′,5-tetrachlorosalicylanilide (up to 100 µmol·L–1) and of the respiration inhibitor mucidin (up to 100 µmol·L–1) in both submerged and surface cultivation. When vegetative mycelia were cultivated on the solid Czapek-Dox medium with yeast autolysate under an anaerobic and CO2-containing atmosphere, the growth was observed only rarely but the microorganism survived as long as 3 months under these conditions. Major products of metabolism of both aerobic and anaerobic submerged my

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Schühle, Karola, and Georg Fuchs. "Phenylphosphate Carboxylase: a New C-C Lyase Involved in Anaerobic Phenol Metabolism in Thauera aromatica." Journal of Bacteriology 186, no. 14 (July 15, 2004): 4556–67. http://dx.doi.org/10.1128/jb.186.14.4556-4567.2004.

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ABSTRACT The anaerobic metabolism of phenol in the beta-proteobacterium Thauera aromatica proceeds via carboxylation to 4-hydroxybenzoate and is initiated by the ATP-dependent conversion of phenol to phenylphosphate. The subsequent para carboxylation of phenylphosphate to 4-hydroxybenzoate is catalyzed by phenylphosphate carboxylase, which was purified and studied. This enzyme consists of four proteins with molecular masses of 54, 53, 18, and 10 kDa, whose genes are located adjacent to each other in the phenol gene cluster which codes for phenol-induced proteins. Three of the subunits (54, 53,

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Gaudêncio, Fabrício Nascimento, Vinícius Menezes Tunholi-Alves, Mariana Gomes Lima, Patrícia Silva Gôlo, Isabele da Costa Angelo, Rosane Nora Castro, Adivaldo Henrique da Fonseca, Fabio Barbour Scott, and Jairo Pinheiro. "Alterations in the oxidative metabolism of Rhipicephalus (Boophilus) microplus ticks in response to exposure to the insect growth regulator fluazuron." Revista Brasileira de Parasitologia Veterinária 25, no. 1 (March 11, 2016): 54–60. http://dx.doi.org/10.1590/s1984-29612016006.

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Abstract Aiming to characterize the potential off-target effects of fluazuron on ticks, biochemical analyses were conducted to evaluate changes in the carbohydrate metabolism of Rhipicephalus (Boophilus) microplus ticks after exposure to fluazuron. Hemolymph and fat body were collected from female ticks before and after (4, 8 and 15 days) exposure to fluazuron. Spectrophotometric analyses were done to quantify glucose concentration and lactate dehydrogenase (LDH) activity in the hemolymph and the concentration of glycogen in the tick’s fat body. High Performance Liquid Chromatography (HPLC) wa

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Morton, Allyson M., Jeremy D. Furtado, Jane Lee, William Amerine, Michael H. Davidson, and Frank M. Sacks. "The effect of omega-3 carboxylic acids on apolipoprotein CIII−containing lipoproteins in severe hypertriglyceridemia." Journal of Clinical Lipidology 10, no. 6 (November 2016): 1442–51. http://dx.doi.org/10.1016/j.jacl.2016.09.005.

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Varynskyi, B. О. "Optimization of detection range of hydrazides of carboxylic acids and 2-acylhydrazinсarbothioamids by HPLC-ECI-MC". Farmatsevtychnyi zhurnal, № 4 (4 вересня 2018): 59–64. http://dx.doi.org/10.32352/0367-3057.4.15.02.

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Elaboration of control steps of hydrazids and сarbothioamids, raw materials in the synthesis of 1,2,4-triazol-3-yl-thioacetate acids, potential drug substances on research and production stage is an important task. The study of adsorption, distribution, metabolism and excretion of these substances has great importance. The HPLC-MS is the most universal and selective method used in these cases. The aim of our study was to determine the optimal conditions for mass spectrometric detection of ionization in electrospray in HPLC-MS іzonіcotinohydrazide, furan-2-carbohydrazide, 2-morpholinoacet

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Ribot, Joan, Andrea Arreguín, Ondrej Kuda, Jan Kopecky, Andreu Palou, and Maria Luisa Bonet. "Novel Markers of the Metabolic Impact of Exogenous Retinoic Acid with A Focus on Acylcarnitines and Amino Acids." International Journal of Molecular Sciences 20, no. 15 (July 25, 2019): 3640. http://dx.doi.org/10.3390/ijms20153640.

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Treatment with all-trans retinoic acid (ATRA), the carboxylic form of vitamin A, lowers body weight in rodents by promoting oxidative metabolism in multiple tissues including white and brown adipose tissues. We aimed to identify novel markers of the metabolic impact of ATRA through targeted blood metabolomics analyses, with a focus on acylcarnitines and amino acids. Blood was obtained from mice treated with a high ATRA dose (50 mg/kg body weight/day, subcutaneous injection) or placebo (controls) during the 4 days preceding collection. LC-MS/MS analyses with a focus on acylcarnitines and amino

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Paiva, Sandra, Dita Strachotová, Helena Kučerová, Otakar Hlaváček, Sandra Mota, Margarida Casal, Zdena Palková, and Libuše Váchová. "The transport of carboxylic acids and important role of the Jen1p transporter during the development of yeast colonies." Biochemical Journal 454, no. 3 (August 29, 2013): 551–58. http://dx.doi.org/10.1042/bj20120312.

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On solid substrates, yeast colonies pass through distinct developmental phases characterized by the changes in pH of their surroundings from acidic to nearly alkaline and vice versa. At the beginning of the alkali phase colonies start to produce ammonia, which functions as a quorum-sensing molecule inducing the reprogramming of cell metabolism. Such reprogramming includes, among others, the activation of several plasma membrane transporters and is connected with colony differentiation. In the present study, we show that colony cells can use two transport mechanisms to import lactic acid: a ‘sa

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De Bruin, Tjerk, Hong Yang, Mats Kvarnström, Torbjörn Lundström, and Michael Davidson. "Omega-3 Carboxylic Acids In Severe Hypertriglyceridemia With Triglycerides Greater Than 885mg/Dl (>10mmol/L)." Journal of Clinical Lipidology 10, no. 3 (May 2016): 702. http://dx.doi.org/10.1016/j.jacl.2016.03.077.

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Wu, Qikui, Xue Zhao, Chen Chen, Zihan Zhang, and Fangyuan Yu. "Metabolite Profiling and Classification of Developing Styrax tonkinensis Kernels." Metabolites 10, no. 1 (January 1, 2020): 21. http://dx.doi.org/10.3390/metabo10010021.

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Background: Styrax tonkinensis is an economic tree species with high timber, medicine, oil, and ornamental value. Its seed, containing a particularly high oil content, are widely studied for their biodiesel properties by nutritional components and oil body ultrastructure. However, their comprehensive biochemical compositions have not been studied. Methods: During S. tonkinensis kernel development, we collected samples from four time points for metabolite profiling and classification through gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry. Results: A total of 18

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Klyachenko, O. L., and A. F. Likhanov. "Content of Triterpene saponins and phenolics compounds in leaves of in vitro Beta vulgaris L. genotypes." Ukrainian Journal of Ecology 7, no. 4 (December 28, 2017): 564–68. http://dx.doi.org/10.15421/2017_161.

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<p>An important element in plants adaptation to adverse environmental stress factors is the synthesis of secondary metabolites which involved in formation for the constitutional stability of plant organism. Saponins play an important role in the regulation of metabolic processes and the development of adaptive and defense plant reactions. Triterpene saponins have pronounced surface-active properties, increase activity of certain enzymes and perform antioxidant function, which in general determines their important role in formation of a common nonspecific plant resistance system. Sugar be

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Peters, Franziska, Michael Rother, and Matthias Boll. "Selenocysteine-Containing Proteins in Anaerobic Benzoate Metabolism of Desulfococcus multivorans." Journal of Bacteriology 186, no. 7 (April 1, 2004): 2156–63. http://dx.doi.org/10.1128/jb.186.7.2156-2163.2004.

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ABSTRACT The sulfate-reducing bacterium Desulfococcus multivorans uses various aromatic compounds as sources of cell carbon and energy. In this work, we studied the initial steps in the aromatic metabolism of this strictly anaerobic model organism. An ATP-dependent benzoate coenzyme A (CoA) ligase (AMP plus PPi forming) composed of a single 59-kDa subunit was purified from extracts of cells grown on benzoate. Specific activity was highest with benzoate and some benzoate derivatives, whereas aliphatic carboxylic acids were virtually unconverted. The N-terminal amino acid sequence showed high si

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Kuzina, Vera, and Enrique Cerdá-Olmedo. "Modification of Sexual Development and Carotene Production by Acetate and Other Small Carboxylic Acids in Blakeslea trispora and Phycomyces blakesleeanus." Applied and Environmental Microbiology 72, no. 7 (July 2006): 4917–22. http://dx.doi.org/10.1128/aem.02845-05.

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ABSTRACT In Phycomyces blakesleeanus and Blakeslea trispora (order Mucorales, class Zygomycetes), sexual interaction on solid substrates leads to zygospore development and to increased carotene production (sexual carotenogenesis). Addition of small quantities of acetate, propionate, lactate, or leucine to mated cultures on minimal medium stimulated zygospore production and inhibited sexual carotenogenesis in both Phycomyces and Blakeslea. In Blakeslea, the threshold acetate concentration was <1 mmol/liter for both effects, and the concentrations that had one-half of the maximal effect were

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Pretorius, Chanel J., Fidele Tugizimana, Paul A. Steenkamp, Lizelle A. Piater, and Ian A. Dubery. "Metabolomics for Biomarker Discovery: Key Signatory Metabolic Profiles for the Identification and Discrimination of Oat Cultivars." Metabolites 11, no. 3 (March 12, 2021): 165. http://dx.doi.org/10.3390/metabo11030165.

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The first step in crop introduction—or breeding programmes—requires cultivar identification and characterisation. Rapid identification methods would therefore greatly improve registration, breeding, seed, trade and inspection processes. Metabolomics has proven to be indispensable in interrogating cellular biochemistry and phenotyping. Furthermore, metabolic fingerprints are chemical maps that can provide detailed insights into the molecular composition of a biological system under consideration. Here, metabolomics was applied to unravel differential metabolic profiles of various oat (Avena sat

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Wu, Miao, Ming Zhang, Wei Ding, Lin Lan, Zhilin Liu, Lingzhan Miao, and Jun Hou. "Microbial Carbon Metabolic Functions in Sediments Influenced by Resuspension Event." Water 13, no. 1 (December 23, 2020): 7. http://dx.doi.org/10.3390/w13010007.

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Microorganisms in sediments are an important part of the aquatic ecosystem, and their functional activities are sensitive to external environmental pressure. Shallow lakes are characterized by frequent sediment resuspension events, leading to large amounts of nutrients being released. However, information about the potential impacts of sediment resuspension events on the functional activities of microbial communities is limited. In this study, the responses of microbial carbon metabolism in sediments under different wind–wave disturbance were analyzed by BIOLOG ECO microplates. The results sho

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Reithuber, Elisabeth, Priyanka Nannapaneni, Olena Rzhepishevska, Anders E. G. Lindgren, Oleksandr Ilchenko, Staffan Normark, Fredrik Almqvist, Birgitta Henriques-Normark, and Peter Mellroth. "The Bactericidal Fatty Acid Mimetic 2CCA-1 Selectively Targets Pneumococcal Extracellular Polyunsaturated Fatty Acid Metabolism." mBio 11, no. 6 (December 15, 2020): e03027-20. http://dx.doi.org/10.1128/mbio.03027-20.

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ABSTRACTStreptococcus pneumoniae, a major cause of pneumonia, sepsis, and meningitis worldwide, has the nasopharynges of small children as its main ecological niche. Depletion of pneumococci from this niche would reduce the disease burden and could be achieved using small molecules with narrow-spectrum antibacterial activity. We identified the alkylated dicyclohexyl carboxylic acid 2CCA-1 as a potent inducer of autolysin-mediated lysis of S. pneumoniae, while having low activity against Staphylococcus aureus. 2CCA-1-resistant strains were found to have inactivating mutations in fakB3, known to

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Sener, A., F. Malaisse-Lagae, C. G. Östenson, and W. J. Malaisse. "Metabolism of endogenous nutrients in islets of Goto-Kakizaki (GK) rats." Biochemical Journal 296, no. 2 (December 1, 1993): 329–34. http://dx.doi.org/10.1042/bj2960329.

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The metabolism of endogenous nutrients was examined in pancreatic islets of control and Goto-Kakizaki (GK) rats. At the ultrastructural level, no glycogen was found in the islet cells of GK rats, a situation similar to that prevailing in normal islets. Likewise, by measuring the output of L-lactate from islets first incubated at 16.7 mM D-glucose and then at 2.8 mM D-glucose, no evidence of glycogenolysis was found in the islets of GK rats. The production of NH4+ and that of 14CO2 from islets prelabelled with either L-[U-14C]glutamine or [U-14C]palmitate were higher, however, in GK than in con

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Gimeno, Isabel, Pablo García-Manrique, Susana Carrocera, Cristina López-Hidalgo, Luis Valledor, David Martín-González, and Enrique Gómez. "The Metabolic Signature of In Vitro Produced Bovine Embryos Helps Predict Pregnancy and Birth after Embryo Transfer." Metabolites 11, no. 8 (July 27, 2021): 484. http://dx.doi.org/10.3390/metabo11080484.

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In vitro produced (IVP) embryos show large metabolic variability induced by breed, culture conditions, embryonic stage and sex and gamete donors. We hypothesized that the birth potential could be accurately predicted by UHPLC-MS/MS in culture medium (CM) with the discrimination of factors inducing metabolic variation. Day-6 embryos were developed in single CM (modified synthetic oviduct fluid) for 24 h and transferred to recipients as fresh (28 ETs) or frozen/thawed (58 ETs) Day-7 blastocysts. Variability was induced with seven bulls, slaughterhouse oocyte donors, culture conditions (serum + B

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Tola, Adesola J., Amal Jaballi, Hugo Germain, and Tagnon D. Missihoun. "Recent Development on Plant Aldehyde Dehydrogenase Enzymes and Their Functions in Plant Development and Stress Signaling." Genes 12, no. 1 (December 31, 2020): 51. http://dx.doi.org/10.3390/genes12010051.

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Abiotic and biotic stresses induce the formation of reactive oxygen species (ROS), which subsequently causes the excessive accumulation of aldehydes in cells. Stress-derived aldehydes are commonly designated as reactive electrophile species (RES) as a result of the presence of an electrophilic α, β-unsaturated carbonyl group. Aldehyde dehydrogenases (ALDHs) are NAD(P)+-dependent enzymes that metabolize a wide range of endogenous and exogenous aliphatic and aromatic aldehyde molecules by oxidizing them to their corresponding carboxylic acids. The ALDH enzymes are found in nearly all organisms,

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Šindelář, Karel, Jiří Holubek, Emil Svátek, Miroslav Ryska, Martin Valchář, and Miroslav Protiva. "Butaclamol-like neuroleptic agents: Synthesis of 1-(11H-dibenz[b,f]-1,4-oxathiepin-11-yl)methyl-4-isobutylpiperidin-4-ol and of some related compounds." Collection of Czechoslovak Chemical Communications 50, no. 7 (1985): 1484–97. http://dx.doi.org/10.1135/cccc19851484.

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1-(11H-Dibenz[b,f]-1,4-oxathiepin-11-yl)methyl-4-piperidone (XIII), which was obtained from 11H-dibenz[b,f]-1,4-oxathiepin-11-carboxylic acid (VIII) in four steps, was treated with isobutylmagnesium bromide and gave the title compound V in addition to the prevailing quantity of the secondary alcohol VI, i.e. the product of reduction. Synthesis of a series of trisubstituted benzyl phenyl sulfide derivatives XVIII-XXIV, XXVI-XXXI is described; these compounds are potential intermediates in the preparation of 11H-dibenz[b,f]-1,4-oxathiepinacetic acids XVI and XVII. Chloromethylation of 11H-dibenz

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