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Rozprawy doktorskie na temat „Cell microscopy”

Autor: Grafiati
Data publikacji: 21 grudnia 2024
Data aktualizacji: 31 lipca 2025

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1

Jaritz, Fritz Simon. "Single Cell Expansion Microscopy." Thesis, KTH, Tillämpad fysik, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-279445.

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Raabe, Isabel. "Visualization of cell-to-cell communication by advanced microscopy techniques." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-178404.

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In order to maintain a multicellular organism cells need to interact and communicate with each other. Signalling cascades such as the Bone Morphogenic Protein (BMP) and Hedgehog (Hh) signalling pathways therefore play essential roles in development and disease. Intercellular signalling also underlies the function of stem cell niches, signalling microenvironments that regulate behaviour of associated stem cells. Range and intensity of the niche signal controls stem cell proliferation and differentation and must therefore be strictly regulated. The testis and ovary of the fruit fly Drosophila me
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Ronteix, Gustave. "Inferring cell-cell interactions from quantitative analysis of microscopy images." Thesis, Institut polytechnique de Paris, 2021. http://www.theses.fr/2021IPPAX111.

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Les systèmes biologiques sont bien plus que la somme de leurs constituants. En effet, ils sont souvent caractérisés par des comportements macroscopiques complexes résultant de boucles d'interactions et de rétroactions. Par exemple, la régulation et le rejet éventuel des tumeurs par le système immunitaire est le résultat de multiples réseaux de régulation, influençant à la fois le comportement des cellules cancéreuses et immunitaires. Pour simuler ces effets complexes in-vitro, j'ai conçu une puce microfluidique permettant de confronter des sphéroïdes de mélanome à de multiples cellules T et d'
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Sjögren, Florence. "Dermal cell trafficking : from microscopy to microdialysis /." Linköping : Univ, 2005. http://www.bibl.liu.se/liupubl/disp/disp2005/med883s.pdf.

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5

Samsuri, Fahmi B. "Single Cell analysis using AtomicForce Microscopy (AFM)." Thesis, University of Canterbury. Electrical and Computer Engineering, 2010. http://hdl.handle.net/10092/5516.

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Replication of biological cells for the purpose of imaging and analysis under electron and scanning probe microscopy has facilitated the opportunity to study and examine some molecular processes and structures of living cells in a manner that were not possible before. The difficulties faced in direct cellular analysis when using and operating Atomic Force Microscopy (AFM) in situ for morphological studies of biological cells have led to the development of a novel method for biological cell studies based on nanoimprint lithography. The realization of the full potential of high resolution AFM im
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6

Sun, Mingzhai. "Cell mechanics studied using atomic force microscopy." Diss., Columbia, Mo. : University of Missouri-Columbia, 2008. http://hdl.handle.net/10355/5499.

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Thesis (Ph. D.)--University of Missouri-Columbia, 2008.<br>The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file (viewed on June 17, 2009) Vita. Includes bibliographical references.
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7

Nguyen, Tran Thien Dat. "Bayesian Multi-Object Tracking for Cell Microscopy." Thesis, Curtin University, 2021. http://hdl.handle.net/20.500.11937/86947.

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Cell tracking is an essential tool for studying how cells behave and divide under different conditions. This thesis proposes new approaches to track cells and their lineages using random finite set, which allows the tracking errors to be statistically quantified. Additionally, this thesis also explores criteria to rank performance of basic vision task algorithms (e.g., object detection, instance-level segmentation, and tracking), which have not been received proportionate attention from the scientific community.
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8

López, Ayón Gabriela. "Applying a commercial atomic force microscope for scanning near-field optical microscopy techniques and investigation of Cell-cell signalling." Thesis, McGill University, 2010. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=92400.

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The field of research of this thesis is Condensed Matter Physics applied to Biology. Specifically it describes the development of different Atomic Force Microscopy techniques and tools towards the study of living cells in physiological solution. Particular interest is put into the understanding of the influence of noise in the determination of ordered liquid layers above a mica surface - as work towards the study of the role of water and ions in biological processes - and the influence of "diving bell" to boost the Q factor and allow stable imaging and force spectroscopy with tips based on Sca
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9

Makarchuk, Stanislaw. "Measurement of cell adhesion forces by holographic microscopy." Thesis, Strasbourg, 2016. http://www.theses.fr/2016STRAE034/document.

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Les forces mécaniques, générées par la cellule jouent un rôle crucial dans l'adhésion cellulaire, qui est un processus commun à un grand nombre de lignées cellulaires. Afin de mesurer la champ des forces pendant l'adhérence cellulaire, nous utilisons la microscopie de force de traction, où la cellule adhère à la surface plane d'un substrat souple dans le plan. Les forces sont calculées à partir du champ de déplacement mesuré à l'intérieur du substrat sous la cellule. Nous avons construit le microscope, dans lequel nous utilisons des billes sphériques en polystyrène pour mesurer le champ de dép
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10

Magnusson, Klas. "Cell tracking for automated analysis of timelapse microscopy." Thesis, KTH, Signalbehandling, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-53772.

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This document presents an algorithm to automatically perform two dimensional tracking of cells in in-vitro cultures. The developed software handles all the necessary data processing, from preprocessing the images to automaticallytracking the cells and it also provides an interface to manually correct the obtained cell trajectories and functions to analyze the data. The system is developed for, and tested on, muscle stem cells (MuSCs) but it can also be applied to other cell types that look and behave similarly. The software was used in a bio-medical study to investigate the effects on mouse Mu
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11

Round, Andrew Neal. "Atomic force microscopy of plant cell wall polysaccharides." Thesis, University of East Anglia, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.297475.

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12

Pulleine, Ellie Mui Mui. "Developing cell identification methods using atomic force microscopy." Thesis, University of Glasgow, 2017. http://theses.gla.ac.uk/8074/.

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This body of work describes the development of a non-invasive and label-free method for characterization of cell surface markers. The motivation for such a method is the ability to measure cells whilst maintaining function, minimizing contamination and disturbance but enabling downstream applications. The technique would impact on life sciences applications including; phenotype identification of both individual and populations of cells, dynamic measurement of cellular response and monitoring cell-microenvironment interactions. The method described centers on molecular recognition interactions
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13

Rong, Guoxin. "Probing cell membrane dynamics using plasmon coupling microscopy." Thesis, Boston University, 2013. https://hdl.handle.net/2144/12840.

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Thesis (Ph.D.)--Boston University PLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you.<br>The plasma membrane of mammalian cells is depicted as a two-dimensional hybrid material which is compartmentalized into submicron-sized domains. These membrane domains play a pivotal role in cellular signaling
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14

Tomalik, Edyta. "Image-based Microscale Particle Velocimetry in Live Cell Microscopy." Thesis, Blekinge Tekniska Högskola, Institutionen för programvaruteknik, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:bth-2564.

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Background: Nowadays, one of the medical problem is rolling cell adhesion. Rolling cell adhesion is a complex process that requires the analysis of the challenging environment such as body fluid and is the process responsible for recruiting the cell to specific organs. In order to explore the rolling cell adhesion, mathematical model is proposed. Different image processing methods are created, such as optical flow - Lucas Kanade algorithm, and other type of methods related to mechanical fluid, namely PIV (Particle Image Velocimetry). Aim: The aim of this master thesis is the identification of
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15

Morris, Sheila. "Atomic force microscopy studies of plant cell wall components." Thesis, University of Bristol, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.420915.

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16

Bartolini, Luca <1989&gt. "Investigation of Cell-material Interactions by Scanning Probe Microscopy." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2017. http://amsdottorato.unibo.it/8198/1/bartolini_luca_tesi.pdf.

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The morphology of cells changes consistently with the surface where they adhere. As reported in the literature, surfaces with micrometric and nanometric patterns affect the cell morphology, as well as surfaces with peculiar chemical functionalities. In order to control both morphology and chemistry of the surface, mono-molecular layers of small organic molecules (specifically Pentacene, α-sexithiophene and PDI8-CN2) were deposited on SiOx substrates by means of Organic Molecular Beam Epitaxy (OMBE). Through the partial annealing method, SiOx substrates were fully covered with a mono-molecular
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17

Arora, Bhupinder S. "Detection of polysaccharides on a bacterial cell surface using Atomic Force Microscopy." Link to electronic thesis, 2003. http://www.wpi.edu/Pubs/ETD/Available/etd-0826103-011111.

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Thesis (M.S.)--Worcester Polytechnic Institute.<br>Keywords: Leuconostoc mesenteroides NIRC1542; Atomic Force Microscope; Pseudomonas putida KT2442; Adhesion. Includes bibliographical references (p. 75-83).
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18

Harvey, Taylor R. "Assessment of VE-Cadherin Stability at Endothelial Cell-Cell Junctions Using Photoconvertible Fluorescence Microscopy." Thesis, Albany Medical College, 2018. http://pqdtopen.proquest.com/#viewpdf?dispub=13422975.

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<p> Regulation of barrier function is critical for patients who suffer from inflammatory diseases such as acute respiratory distress syndrome (ARDS) and sepsis. A major regulator of endothelial barrier function is vascular endothelial cadherin (VE-cad). Cellular levels of VE-cad are known to be regulated by p120 catenin. Loss of p120 leads to decreased barrier function as a result of the endocytosis of VE-cad. However, recent work from our lab shows that expression of an endocytic defective VE-cad mutant was not able to rescue barrier function, as measured using transendothelial electrical res
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19

Kosmacek, Elizabeth Anne Ianzini Fiorenza Mackey Michael A. "Live cell imaging technology development for cancer research." [Iowa City, Iowa] : University of Iowa, 2009. http://ir.uiowa.edu/etd/388.

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20

Cheng, Eric. "Investigations into inkjet cell printing hydrodynamics through microscopy imaging techniques." Thesis, University of British Columbia, 2015. http://hdl.handle.net/2429/52776.

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Inkjet bioprinting technology aims to accurately and precisely dispense biological materials in a spatially predefined pattern within a three-dimensional space. The technology has a multitude of applications in the biomedical field such as in drug discovery and tissue or organ engineering. However, there are known limitations in an inkjet nozzle's capabilities in dispensing cells as the cell ejection rate does not follow any predictable distributions. In this work, the cell behaviors within a piezoelectric nozzle due to droplet ejection were classified through high speed brightfield imaging. W
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21

Panday, Namuna. "Scanning Ion Conductance Microscopy for Single Cell Imaging and Analysis." FIU Digital Commons, 2017. http://digitalcommons.fiu.edu/etd/3477.

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Most biological experiments are performed on an ensemble of cells under the assumption that all cells are identical. However, recent evidence from single cells studies reveals that this assumption is incorrect. Individual cells within the same generation may differ dramatically, and these differences have important consequences for the health and function of the entire living body. I have used Scanning Ion Conductance Microscopy (SICM) for imaging and analysis of topographical change of single cell membrane, which is difficult to be revealed by optical microscopes. Morphological change in the
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22

CHIGHIZOLA, MATTEO. "INVESTIGATION OF CELL-MICROENVIRONMENT INTERACTIONS BY ATOMIC FORCE MICROSCOPY TECHNIQUES." Doctoral thesis, Università degli Studi di Milano, 2021. http://hdl.handle.net/2434/819943.

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The role of forces is fundamental in a wide variety of biological processes, such as cell adhesion, migration, proliferation and differentiation. The ability of cells to perceive the nanotopographical features of the surrounding microenvironment (i.e. the extracellular matrix, ECM), called mechanotransduction, is mediated by specific trans-membrane proteins, called integrins, clustered together in Integrin Adhesion Complexes (IAC). Unraveling which mechanical and nanoscale morphological properties of the ECM determine the IAC composition and tune specific cellular response, is particularly cha
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23

Altinoglu, Ipek. "Organization of Bacterial Cell Pole." Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS367/document.

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Chez les bactéries, les pôles cellulaires servent de domaines subcellulaires impliqués dans plusieurs processus cellulaires. Chez l’agent pathogène du choléra, Vibrio cholerae, en forme de bâtonnet incurvé, le pole contenant l’unique flagelle est impliqué dans la virulence. La protéine d’ancrage polaire HubP interagit avec plusieurs ATPases telles que ParA1 (ségrégation des chromosomes), ParC (localisation polaire du système de chimiotaxie) et FlhG (biosynthèse des flagelles), organisant ainsi l'identité polaire de V. cholerae. Cependant, les mécanismes moléculaires exacts de cet ancrage polai
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24

Lähdesmäki, Ilkka Johannes. "Flow injection methods for drug-receptor interaction studies, based on probing cell metabolism /." Thesis, Connect to this title online; UW restricted, 1999. http://hdl.handle.net/1773/8590.

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25

Zhang, Weimin. "Topics in living cell miultiphoton laser scanning microscopy (MPLSM) image analysis." Texas A&M University, 2006. http://hdl.handle.net/1969.1/4412.

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Multiphoton laser scanning microscopy (MPLSM) is an advanced fluorescence imaging technology which can produce a less noisy microscope image and minimize the damage in living tissue. The MPLSM image in this research is the dehydroergosterol (DHE, a fluorescent sterol which closely mimics those of cholesterol in lipoproteins and membranes) on living cell's plasma membrane area. The objective is to use a statistical image analysis method to describe how cholesterol is distributed on a living cell's membrane. Statistical image analysis methods applied in this research include image segmentation/c
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26

Lindmark, Sofia. "Cell Tracking in Microscopy Images Using a Rao-Blackwellized Particle Filter." Thesis, Uppsala universitet, Avdelningen för visuell information och interaktion, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-236769.

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Analysing migrating cells in microscopy time-lapse images has already helped the understanding of many biological processes and may be of importance in the development of new medical treatments. Today’s biological experiments tend to produce a huge amount of dynamic image data and tracking the individual cells by hand has become a bottleneck for the further analysis work. A number of cell tracking methods have therefore been developed over the past decades, but still many of the techniques have a limited performance. The aim of this Master Project is to develop a particle filter algorithm that
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Siamantouras, Eleftherios. "Nanomechanical investigation of soft biological cell adhesion using atomic force microscopy." Thesis, University of Warwick, 2014. http://wrap.warwick.ac.uk/62745/.

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Cell-to-cell adhesion is critically important for the improved secretory function of endocrine pancreatic beta (β)-cells and for the progression of fibrosis in the renal proximal tubule in Diabetic Nephropathy. In this research project the effects of specific biochemical treatment on functional cell-to-cell adhesion and single cell mechanics were systematically investigated. Atomic Force Microscopy (AFM) Single Cell Force Spectroscopy was applied to quantitatively characterise E-cadherin mediated surface ligation and cytoskeletal reorganisation in the pancreatic mouse insulinoma MIN6 and human
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28

Harriman, Oliver Leon Jacobs. "A system-level approach to single-molecule live-cell fluorescence microscopy." Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:81425bd2-6bc3-489e-b159-a2590ffffbb1.

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In this work a system-level approach was taken to the single-molecule fluorescence microscopy of living cells. This primarily involved the unification of relevant information within appropriately structured artefacts that were used to inform and enhance experimentation. Initially the diversity of emerging single-molecule techniques was reviewed and presented with a novel article structure to suit the purpose of designing an experiment (Harriman and Leake 2011). Techniques were grouped by the type of information they could access, rather than the standard organisation centred on the techniques
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MacKay, Gillian E. "Analysis of cell allocation in GFP chimeric blastocysts by confocal microscopy." Thesis, University of Edinburgh, 2003. http://hdl.handle.net/1842/29238.

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This study combined confocal microscopy with the use of a tau-GFP (green fluorescent protein) transgenic mouse strain to study cell fate in two main types of chimeric blastocysts. In each case one component of the chimera was known to contribute poorly to the fetal lineage at later stages. The experiments were designed to test whether this was due to non-random allocation to different tissues at the blastocyst stage. The initial part of this thesis involved the establishment of confocal microscopy techniques and the characterisation of two novel tau-GFP transgenic mouse strains. A method of cu
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30

Wong, Tsz-wai Terence, and 黃子維. "Optical time-stretch microscopy: a new tool for ultrafast and high-throughput cell imaging." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hub.hku.hk/bib/B5066234X.

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The exponential expansion in the field of biophotonics over the past half-century has been leading to ubiquitous basic science investigations, ranging from single cell to brain networking analysis. There is also one biophotonics technology used in clinic, which is optical coherence tomography, mostly for high-speed and high-resolution endoscopy. To keep up such momentum, new biophotonics technologies should be aiming at improving either the spatial resolution or temporal resolution of optical imaging. To this end, this thesis will address a new imaging technique which has an ultra-high tempor
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31

Dahan, Sophie. "Intracellular proteinmembrane trafficking : evaluation of the Golgi and endosomal apparatus by cryoimmune electron microscopy." Thesis, McGill University, 1995. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=29387.

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Protein trafficking events in the secretory and endosomal apparatus were evaluated in rat liver hepatocytes by EM immunogold cytochemistry. The cellular distribution of apolipoprotein E and other abundant hepatic secretory and endosomal proteins was quantitatively examined in ultrathin cryosections of liver hepatocytes. Under steady-state conditions, apoE was concentrated within the Golgi apparatus, along sinusoidal plasma membrane microvilli and within all components of the endosomal apparatus, as evaluated immunocytochemically and confirmed by quantitative immunoblotting of organelles isolat
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32

Khanduja, Nimisha. "Processive Acceleration of Actin Barbed End Assembly by N-WASP." Diss., Virginia Tech, 2014. http://hdl.handle.net/10919/54933.

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Actin-based cell motility plays crucial roles throughout the lifetime of an organism. The dynamic rearrangement of the actin cytoskeleton triggers a plethora of cellular processes including cellular migration. Neural Wiskott Aldrich syndrome protein (N-WASP) is involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. N-WASP activated actin polymerization drives extension of invadopodia and podosomes into the basement layer. In addition to activating Arp2/3 complex, N-WASP binds actin filament barbed ends, and both N-WASP and barbed ends are tightly clus
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33

Balanant, Marie-Anne. "Experimental studies of red blood cells during storage." Thesis, Queensland University of Technology, 2018. https://eprints.qut.edu.au/119221/1/Marie-Anne_Balanant_Thesis.pdf.

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This project used mechanical testing and imaging techniques to understand how red blood cells age in an in vitro environment, and identified markers of red blood cell product quality. The damage caused to the cells by cold storage could lead to a reduced transfusion efficiency and potential improvements to current storage protocols were proposed as a result of this research.
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34

Tobin, Mark James. "Hormone-receptor interaction by time resolved fluorescence and image analysis." Thesis, University of Salford, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.238743.

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35

Olofsson, Per Erik. "Microscopy-based single-cell in vitro assays for NK cell function in 2-D and 3-D." Doctoral thesis, KTH, Cellulär biofysik, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-199571.

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Natural killer (NK) cells are effector cells of the innate immune system that are responsible for mediating cellular cytotoxicity against virally infected or neoplastically transformed cells. NK cell subsets are defined by their expression of certain cell-surface markers, and are usually related to activation and developmental status. However, how distinct NK cell phenotypes correlate with behavior in NK-target interactions is less widely characterized. There is therefore a need to study NK cell behavior down at the single-cell level. One aim of this thesis is to approach methods that quantita
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36

Stayton, Isaac Alexander. "Investigation of the interactions between selected nanoparticles and human lung carcinoma cells at the single cell and single particle level." Diss., Rolla, Mo. : Missouri University of Science and Technology, 2009. http://scholarsmine.mst.edu/thesis/pdf/Stayton_09007dcc8065344d.pdf.

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Thesis (Ph. D.)--Missouri University of Science and Technology, 2009.<br>Vita. The entire thesis text is included in file. Title from title screen of thesis/dissertation PDF file (viewed April 29, 2009) Includes bibliographical references.
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Joensuu, Jenny. "Online Image Analysis of Jurkat T Cells using in situ Microscopy." Thesis, Linköpings universitet, Institutionen för fysik, kemi och biologi, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-153313.

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Cell cultivation in bioreactors would benefit from developed monitoring systems with online real-time imaging to evaluate cell culture conditions and processes. This opportunity can be provided with the newly developed in situ Microscope also called ISM. The ISM probe is mounted into the wall of a bioreactor and consists of a measurement zone with an illuminating light source to obtain real-time images of moving cells in suspension. The instrument is linked to advanced imaging analysis software which can be specifically adapted for the objects in study. The aim of this project is to analyze th
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38

Spanoudis, Catherine M. "Cell Division Regulation in Staphylococcus aureus." Scholar Commons, 2017. http://scholarcommons.usf.edu/etd/7090.

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Cell division is a fundamental biological process that occurs in all kingdoms of life. Our understanding of cell division in bacteria stems from studies in the rod-shaped model organisms: Gram-negative Escherichia coli and Gram-positive Bacillus subtilis. The molecular underpinnings of cell division regulation in non-rod-shaped bacteria remain to be studied in detail. Rod-shaped bacteria possess many positive and negative regulatory proteins that are essential to the proper placement of the division septa and ultimately the production of two identical daughter cells, many
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Thomas, Gawain M. "The Role of Integrins in Cellular Response to Mechanical Stimuli." Digital WPI, 2017. https://digitalcommons.wpi.edu/etd-theses/114.

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Tissue cells exhibit varying responses according to the stiffness of their extracellular matrix (ECM). The mechanism of this stiffness sensing is not fully understood; however, it is known that cells probe stiffness by applying intracellular force to the ECM via integrin-mediated focal adhesions. The bonds between integrins and ECM have been described as “catch bonds�, and it is unclear how ECM viscoelasticity affects these bonds. We have observed the effects of ECM stiffness on the binding strength of integrins to ECM ligands by measuring the dissociation force of individual integrin-liga
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40

Shojaeizadeh, Mina. "An improved approach for cell traction force microscopy using a continuous hydrogel." Digital WPI, 2013. https://digitalcommons.wpi.edu/etd-theses/1199.

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"In this thesis, a cell traction force microscopy method is developed for measuring traction forces of connective tissue cells. This method includes an improved methodology in traction force microscopy of live cells cultured on an elastic substrate. Tissue cells, such as skin and muscle cells respond to the mechanical stimuli of their microenvironment by adhering to their substrate and exerting forces on the proteins of the extracellular matrix (ECM). These forces are called cell traction forces. Fibroblasts are grown on polyacrylamide (PA) gels embedded with fluorescent beads and coated with
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41

Ray, Lucille Alexandria. "Live single cell fluorescence microscopy; from antibiotic resistance detection to mitochondrial dysfunction." University of Akron / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=akron1597342775751888.

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42

Karuna, Arnica. "Applications of coherent anti-Stokes Raman scattering (CARS) microscopy to cell biology." Thesis, Cardiff University, 2016. http://orca.cf.ac.uk/94088/.

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Traditionally, many advances in the field of biology have been driven by optical microscopy based techniques which reveal morphological information about the samples under study [1, 2, 3, 4]. The scope of the applications of these methods is limited due to the lack of contrast from most biological materials (cells and tissues) which are transparent to visible light. The introduction of extraneous materials (such as fluorescent quantum dots or other fluorescent proteins/labels) with affinity towards certain sub-cellular components which are then imaged, has emerged as a popular and powerful met
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Huh, Seungil. "Toward an Automated System for the Analysis of Cell Behavior| Cellular Event Detection and Cell Tracking in Time-lapse Live Cell Microscopy." Thesis, Carnegie Mellon University, 2013. http://pqdtopen.proquest.com/#viewpdf?dispub=3538985.

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<p>Time-lapse live cell imaging has been increasingly employed by biological and biomedical researchers to understand the underlying mechanisms in cell physiology and development by investigating behavior of cells. This trend has led to a huge amount of image data, the analysis of which becomes a bottleneck in related research. Consequently, how to efficiently analyze the data is emerging as one of the major challenges in the fields. </p><p> Computer vision analysis of non-fluorescent microscopy images, representatively phase-contrast microscopy images, promises to realize a long-term monito
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Wang, Ruixing. "STED-fluorescence correlation spectroscopy for dynamic observations in cell biology : from theoretical to practical approaches." Thesis, Aix-Marseille, 2018. http://www.theses.fr/2018AIXM0163/document.

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Les techniques de super-résolution offrent un nouvel aperçu de la description de l'organisation moléculaire dynamique de la membrane plasmique. Parmi ces techniques, la microscopie par déplétion d'émission stimulée (stimulated emission depletion, STED) dépasse la limite de diffraction optique et atteint une résolution de quelques dizaines de nanomètres. Il est une technique polyvalente qui peut être combinée avec d'autres techniques telles que la spectroscopie par corrélation de fluorescence (fluorescence correlation spectroscopy, FCS), fournissant des résolutions spatiales et temporelles élev
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Everett, William Neil. "Evanescent wave and video microscopy methods for directly measuring interactions between surface-immobilized biomolecules." Thesis, [College Station, Tex. : Texas A&M University, 2007. http://hdl.handle.net/1969.1/ETD-TAMU-1585.

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Ball, Francis John. "Development of a microfluidic device for single cell analysis using FT-IR microscopy." Thesis, University of Manchester, 2013. https://www.research.manchester.ac.uk/portal/en/theses/development-of-a-microfluidic-device-for-single-cell-analysis-using-ftir-microscopy(595222c5-f908-49a2-8fa9-6cfddc96e462).html.

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Prostate cancer is the second most common cause of cancer fatalities in males in the UK (2006) [1]. Therefore any advances in the diagnosis or screening for this form of cancer will yield significant benefits in the treatment of this disease. FT-IR has already been successfully used to assess and grade prostate biopsies by Gazi et al 2006 [2]. The collection of prostate biopsy is however a highly invasive procedure and as current screening methods are highly sensitive, but not very specific, large numbers of patients are referred for biopsy procedures that later come back as negative for prost
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Myers, Janette Bernadette. "Application of Single Particle Electron Microscopy to Native Lens Gap Junctions and Intrinsically Disordered Signaling Complexes." PDXScholar, 2019. https://pdxscholar.library.pdx.edu/open_access_etds/5008.

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Gap junctions are a class of membrane proteins that facilitate cell-to-cell communication by forming channels that directly couple the cytoplasm of neighboring cells. The channels are composed of monomers called connexins. Humans express 21 connexin isoforms in a cell-type specific fashion, and each isoform has distinct mechanisms of permeation and regulation. Co-assembly of multiple isoforms into a single intercellular channel can change channel properties, such as conductance and selectivity to substrates (e.g., ions, metabolites and signaling molecules). However, the mechanistic basis for t
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Wang, Congchao. "Automated Tracking of Mouse Embryogenesis from Large-scale Fluorescence Microscopy Data." Diss., Virginia Tech, 2021. http://hdl.handle.net/10919/103595.

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Recent breakthroughs in microscopy techniques and fluorescence probes enable the recording of mouse embryogenesis at the cellular level for days, easily generating terabyte-level 3D time-lapse data. Since millions of cells are involved, this information-rich data brings a natural demand for an automated tool for its comprehensive analysis. This tool should automatically (1) detect and segment cells at each time point and (2) track cell migration across time. Most existing cell tracking methods cannot scale to the data with such large size and high complexity. For those purposely designed for e
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Distasi, Matthew R. "The 3D characterization of the annulate lamellae : the development of a new methodology incorporating 3D-anaglyph techniques and serial transmission electron microscopy." Virtual Press, 2003. http://liblink.bsu.edu/uhtbin/catkey/1266020.

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CRESTANI, MICHELE. "MECHANOPROPERTIES, HETEROGENEITY AND CELL MIGRATION IN GLIOBLASTOMA." Doctoral thesis, Università degli Studi di Milano, 2022. https://hdl.handle.net/2434/946015.

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Glioblastomas (GBMs) are primary brain tumors endowed with inter- and intra-patient heterogeneity and extreme di↵usivity. As heterogeneity is studied with genomic and transcriptomic analysis, little is known on how it is reflected on cell migration, mechanoproperties and motility modes. Generally, the tumor cells invade the brain moving on brain vasculature or white matter tracks: Patient-Derived Xenograft (PDX) has been a standard to reproduce them in order to study GBM invasion. However, PDX presents many disadvantages, including time consumption, hard standardization, high cost and e
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