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Rozprawy doktorskie na temat „Cell suspension”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 25 lipca 2025

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1

Menges, Margit. "Synchronisation of Arabidopsis cell suspension cultures." Thesis, University of Cambridge, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.620602.

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Green, Martha Alexandra. "Apoplastic ascorbate metabolism in rose cell suspension cultures." Thesis, University of Edinburgh, 2003. http://hdl.handle.net/1842/14944.

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Endogenous intraprotoplasmic ascorbate in rose cell suspension cultures as a model system ranged from 0.05 mmol kg<sup>-1</sup> in 0-d-old cultures to 1.1 mmol kg<sup>-1</sup> in 5-d-old cultures. Apoplastic ascorbate was estimated as 0.5 and 8 μM in 0- and 5-d-old cultures respectively, indicating that ascorbate is endogenous to, and may be metabolised within, the apoplast. Exogenous (apoplastic) 1 mM L-[1-<sup>14</sup>C]ascorbate was almost completely consumed (metabolised and/or taken up) by rose cultures within 8 hours of administration. Total <sup>14</sup>C was removed from medium but slo

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Chakrabortee, Sohini. "Characterisation of cytokinin responses in arabidopsis cell suspension culture." Thesis, University of Cambridge, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.613927.

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Smith, Rachel C. "Xyloglucan endotransglycosylation in the apoplast of plant cell suspension cultures." Thesis, University of Edinburgh, 1992. http://hdl.handle.net/1842/12139.

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Xyloglucan is thought to form cross-links between cellulose microfibrils within the plant cell wall. The hydrolysis of these 'tethers' may be involved in wall loosening, and a control of cell expansion. Transglycosylation reactions between xyloglucan 'tethers' has for some years been suggested as an alternative mechanism to hydrolysis by which the plant cell wall may be loosened. The work described in this thesis is an investigation of this hypothesis. Spinach (<i>Spinacia oleracea</i> L.) cell suspension cultures were incubated with [<i>xylosyl</i>-<SUP>3</SUP>H]xyloglucan nonasaccharide (XG9

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5

Eberhardt, Thomas Leonard. "Characterization of lignin deposition in Pinus taeda L. cell suspension cultures." Diss., This resource online, 1992. http://scholar.lib.vt.edu/theses/available/etd-07282008-134210/.

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Athwal, Gurdeep Singh. "Glutamate dehydrogenase, its role, regulation and characterisation in carrot cell suspension cultures." Thesis, University College London (University of London), 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307584.

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7

Van, der Westhuizen Andries P. P. "The evaluation of solids suspension in a pilot scale mechanical flotation cell." Master's thesis, University of Cape Town, 2004. http://hdl.handle.net/11427/5390.

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Includes bibliographical references.<br>The central step in flotation is particle collection, with solids suspension together with gas dispersion and reagent mixing as necessary preconditions for particle collection. Solids suspension is therefore often identified as an important subprocess for effective flotation. Yet, surprisingly little work has been published on solids suspension in mechanical flotation cells, especially more recent studies since the advent of round mechanical flotation cells and the subsequent dramatic increases in maximum cell sizes are largely lacking.

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Riley, Bryan Scott. "The Suspension Cultivation of, and the use of Alternative Cell lines for the In Vitro Cultivation of, Treponema Pallidum Subspecies Pallidum." Thesis, University of North Texas, 1989. https://digital.library.unt.edu/ark:/67531/metadc798117/.

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This study had two objectives: to achieve suspension cultivation of Sf1Ep cells and to develop procedures for achieving the replication of T. pallidum in those cell cultures. Sf1Ep cells have been the sole cell line used for the in vitro cultivation of T. pallidum. A study was undertaken to determine if other cell lines can support growth of T. pallidum. Rabbit skin fibroblasts (RAB-9), nude mouse ear (NME) cells, and normal rebbit testis fibroblasts (RT) were compared to Sf1Ep cells for their ability to support in vitro multiplication of T. pallidum. RAB-9 cells supported multiplication of tr

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9

Kang, Jane. "Migration of blood cells in non-uniform suspension for a dialyzer design." Diss., Georgia Institute of Technology, 2015. http://hdl.handle.net/1853/53871.

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Hemodialysis is a renal replacement therapy that removes waste solutes from the blood stream using concentration gradients across a membrane. In order to overcome several shortcomings and increase the waste removal rate, a new dialyzer (filter) design is proposed in this study. In the new dialyzer design, the blood concurrently flows with a sheath fluid in a micro-fluidic channel. Because the blood stream directly contacts the sheath stream, it is important to prevent blood cell migration from the blood stream to the sheath stream while providing enough time for the waste solutes to diffuse

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10

Moraes, Trevor F. "Purification and characterization of phosphoenolpyruvate carboxylase from Brassica napus (canola) suspension cell cultures." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0001/MQ42669.pdf.

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Waldbillig, David. "Aqueous suspension plasma spraying of yttria stabilized zirconia solid oxide fuel cell electrolytes." Thesis, University of British Columbia, 2010. http://hdl.handle.net/2429/27910.

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In order to meet increasing world energy demand in a sustainable manner, clean and efficient new energy technologies need to be developed. Fuel cells have been proposed as a potential energy conversion technology to help facilitate this transition to cleaner energy. Solid oxide fuel cells (SOFC) in particular are thought to be a practical, near term clean energy technology; however, current state-of-the-art wet ceramic fabrication techniques make SOFC manufacturing labour-intensive, fairly expensive and difficult to automate, and the high firing temperatures required limit the usable materials

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12

Åslund, Ingrid, Samo Lasič, Agnieszka Nowacka, Markus Nilsson, and Daniel Topgaard. "Measuring molecular exchange for water in a yeast cell suspension through NMR diffusometry." Universitätsbibliothek Leipzig, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-190551.

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Kerr, Ellen M. "The synthesis, wall-binding and breakdown of hemicelluloses in maize cell-suspension cultures." Thesis, University of Edinburgh, 2001. http://hdl.handle.net/1842/12355.

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A method was developed to separate protoplasmic contents from the cell wall causing polymer degradation. Alkali treatment (6 M NaOH at 37°C) was shown then to solubilise essentially all remaining (wall-bound) <sup>3</sup>H-polymers. Gel-permeation chromatography (GPC) on Sepharose CL-4B was used to size-fractionate the isolated <sup>3</sup>H-polymers. Alkali treatments, similar to those employed to extract wall-bound <sup>3</sup>H-hemicellulose, were found to minimise aggregation of xylan and xyloglucan caused by sample storage and such treatments were applied to <sup>3</sup>H-polymers from ea

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Åslund, Ingrid, Samo Lasič, Agnieszka Nowacka, Markus Nilsson, and Daniel Topgaard. "Measuring molecular exchange for water in a yeast cell suspension through NMR diffusometry." Diffusion fundamentals 11 (2009) 69, S. 1-2, 2009. https://ul.qucosa.de/id/qucosa%3A14035.

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15

Lagares, Lemos Miguel. "Matlab Based Specific Impedance Spectroscopy Simulator for Suspension of Cells." Thesis, Högskolan i Borås, Institutionen Ingenjörshögskolan, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-19348.

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By means of the analytical formula of specific impedance for the spherical cells in suspensionintroduced by Kenneth S. Cole in 1928, a bioimpedance simulator has been developed for thegeneration of specific impedance spectrums of suspension of cells. With the help of the simulatorthe user can obtain different impedance spectrums according with the biophysical parameters ofthe cell suspension. Then, generate different kind of plots in order to understand and interpret allthe resulting information.With the selection of different values and range of the biophysical parameters to obtain thespectru

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Nealey, Luke T. "The isolation, characterization, and biological testing of xyloglucan from suspension cultured lobloly pine cell spent medium." Diss., Georgia Institute of Technology, 1987. http://hdl.handle.net/1853/5749.

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Zandstra, Peter William. "Cytokine-dependent regulation of human hematopoietic cell self-renewal and differentiation in suspension cultures." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq25194.pdf.

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Montgomery, Sarah Lynn. "Impedance measurement system for embryonic stem cell and embryoid body cultures." Thesis, Atlanta, Ga. : Georgia Institute of Technology, 2008. http://hdl.handle.net/1853/24661.

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19

Gerasymenko, Iryna. "Molecular cloning, heterologous expression and characterization of strictosidine glucosidase from Rauvolfia serpentina cell suspension cultures." [S.l.] : [s.n.], 2002. http://ArchiMeD.uni-mainz.de/pub/2002/0036/diss.pdf.

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20

Byers, Susan D. "Stimulation of microsomal diacylglycerol acyltransferase activity from microspore-derived cell suspension cultures of oilseed rape." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0016/MQ49143.pdf.

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21

Byers, Susan D., and University of Lethbridge Faculty of Arts and Science. "Stimulation of microsomal diacylglycerol acyltransferase activity from microspore-derived cell suspension cultures of oilseed rape." Thesis, Lethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 1999, 1999. http://hdl.handle.net/10133/101.

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Several factors including an unidentified endogenous substance were found to stimulate microsomal diacylglycerol acyltransferase (DGAT, EC 2.3.1.20) from a microspore-derived cell suspension culture of oilseed rape (Brassica napus L. cv Jet Neuf). Mg2+ salts were found to stimulate microsomal DGAT 14 to 23-fold. ATP and CoA were also found to stimulate the enzyme 2.4 and 12 fold respectively, although the effects were decreased in the presence of high Mg2+ concentrations. While microsomal DGAT activity was only slightly increased by the concentration of exogenous diacylglycerol in the reaction

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22

Alyousuf, Saeed Habib Hassan. "Comparison of free amino acid profiles in carrot cell suspension cultures resistant to stress conditions." Diss., The University of Arizona, 1989. http://hdl.handle.net/10150/184631.

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Plant cells resistant to specific amino acid analogs have been reported to accumulate the corresponding free amino acids. The purpose of this study was to determine the concentrations of fifteen free amino acids: alanine, valine, leucine, isoleucine, glutamate, proline, arginine, aspartate, threonine, methionine, lysine, serine, glycine, tryptophan and phenylalanine in Daucus carota cell lines, resistant either to the proline analog azetidine-2 carboxylic acid (A2C), or to the tryptophan analog 5-methyltryptophan (5-MT), or to both the analogs combined. This study also intended to determine if

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23

Lam, Ming-Chi. "In silico dynamic optimisation studies for batch/fed-batch mammalian cell suspension cultures producing biopharmaceuticals." Thesis, Imperial College London, 2008. http://hdl.handle.net/10044/1/45465.

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Mammalian cell cultures are valuable for synthesis of therapeutic proteins and antibodies. They are commonly cultivated in bioindustry in form of large-scale suspension fed-batch cultures. The structure and regulatory responses of mammalian cells are complex, making it challenging to model them for practical process optimisation. The adjustable degrees of freedom in the cell cultures can be continuous variables as well as binary-type variables. The binary-type variables may be irreversible in cases such as cell-cycle arrest. The main aim of this study was to develop a general model for mammali

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24

Parisi, Ann Margaret. "Investigation of Secondary Metabolite Production in Selected Australian Native Species via Plant Cell Suspension Culture." Thesis, Griffith University, 2006. http://hdl.handle.net/10072/366129.

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Natural products and natural-product-derived substances comprised about 35% of the total pharmaceuticals market volume of US$230 billion in 1996 (Wessjohann, 2000). The success of natural-based drugs can be attributed to nature’s ability to induce effects by chemical means and many of these chemicals are able to pass species boundaries to cause an effect. Since plant secondary metabolites have evolved in the interaction with other organisms, many of them have interesting biological or therapeutical activities that are useful to man. In addition to their intriguing chemistry a number of these c

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25

Zagallo, Monica. "Multiscale technologies for human stem cell culture in suspension: applications for hematopoietic and cardiac differentiation." Doctoral thesis, Università degli studi di Padova, 2011. http://hdl.handle.net/11577/3427393.

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Stem cell based therapies have been proposed as promising solutions for the regeneration or replacement of diseased tissues, but their potential benefits are limited mainly by scarse cell availability and clinical concern related to cell quality. Aim of the presented research is to develop technologies for human stem cell culture in order to improve the efficiency and the quality of stem cell products. In particular, a bioeractor with a working volume of 10ml/well and a microstructured hydrogel have been designed to perform cultures of human cord blood-derived hematopoietic stem cells (UCB-der

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Davoren, Jonathan M., and University of Lethbridge Faculty of Arts and Science. "Gene expression in a microspore-derived cell suspension culture of Brassica Napus exhibiting enhanced oil production." Thesis, Lethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 1997, 1997. http://hdl.handle.net/10133/345.

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Triacylglycerol (TAG) production in the microspore derived (MD) cell suspension culture ofBrassica napus L. cv Jet Neuf was enhanced when the sucrose concentration in the growth medium was increased from 2 to 14 % (w/v). mRNA differential display by polymerase chain reaction was used to examine gene expression in cells grown at different sucrose concentrations in order to identify mRNAs which could be associated with oil formation. The anchored primer, T12AA, was used to screen one subset, representing approximately one twelfth of the transcript population, isolated from cultures grown in medi

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Chang, Yu-Hsiang David. "Augmentation of mass transfer through electrical means and nutrient enrichment for suspension and entrapment cell cultures." Thesis, Massachusetts Institute of Technology, 1994. http://hdl.handle.net/1721.1/33503.

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Hao, Dong-Yun. "Studies on nicotine N-demethylation in cell suspension cultures of Nicotiana tabacum L cv. Wisconsin-38." Thesis, University of Edinburgh, 1994. http://hdl.handle.net/1842/13992.

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Radioactive feeding experiments, in which <I>DL</I>-[pyrrolidine-2'-<SUP>14</SUP>C]-nicotine was added to 10 day old cultures, confirmed that the kinetic pattern of this <I>N-</I>demethylation was similar to that of non-radioactive nicotine, and that nornicotine was the major product and was produced intracellularly with a maximum percentage conversion of approximately 70%. The appearance of nornicotine paralleled the disappearance of the added nicotine, although small amounts of four other radioactive-metabolites were observed. One of these metabolites was tentatively identified by GC-MS as <

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Toubal, Malika. "Caractérisation ultrasonore de milieux biologiques en suspension : application aux hématies." Valenciennes, 1996. https://ged.uphf.fr/nuxeo/site/esupversions/0adf1de9-0c5e-4d57-b4c8-ff9d51b13204.

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Notre travail porte sur la caractérisation ultrasonore de suspensions d'hématies à travers l'analyse de l'atténuation et de la vitesse de propagation dans une gamme de fréquences comprise entre 30 et 75 mhz. Parmi les milieux biologiques, nous avons choisi le sang du fait que l'on peut aisément modifier sa composition et ses propriétés physico-chimiques pour en vérifier l'impact sur la propagation de l'onde élastique. Dans le sang, l'absorption résulte de deux processus distincts: l'un macromoléculaire, l'autre corpusculaire. C’est à l'absorption corpusculaire que nous nous sommes intéressés p

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Terrasso, Ana Paula Barreto. "Development of novel human cellular models for neurotoxicity studies." Master's thesis, Faculdade de Ciências e Tecnologia, 2012. http://hdl.handle.net/10362/8488.

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Dissertation to obtain master degree in Genética Molecular e Biomedicina<br>Information currently available on neurotoxicity of chemicals is scarce and there are a growing number of new compounds to be tested. Therefore, new strategies are necessary to identify neurotoxic agents with speed, reliability and respect for animal welfare. The limited availability of primary human brain cells means that there is a need for human cell lines that reliably model human neurons and astrocytes. Despite the advances in stem cell research, numerous challenges must be overcome before this technology can be

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Toler, Tanikka. "Effects of Light Exposure on the Release of Oxygen from Hemoglobin in a Red Blood Cell Suspension." VCU Scholars Compass, 2008. http://scholarscompass.vcu.edu/etd/1637.

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The main function of the cardiovascular system is to deliver a sufficient quantity of oxygenated blood to the tissues, cells, and organs of the body in order to provide the cells with essential nutrients for metabolism and for the removal of waste products. All cells require and utilize oxygen. Oxygen is transported to various cells and tissues via red blood cells flowing through the microcirculation of an organism. Measurement of oxygen transport in the microcirculation has shown that about ten times more oxygen appears to leave the blood of arterioles than can be accounted for by diffusio

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Case, Natasha D. "Oscillatory Compressive Loading Effects On Mesenchymal Progenitor Cells Undergoing Chondrogenic Differentiation In Hydrogel Suspension." Diss., Georgia Institute of Technology, 2005. http://hdl.handle.net/1853/6939.

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Articular cartilage functions to maintain joint mobility. The loss of healthy, functional articular cartilage due to osteoarthritis or injury can severely compromise quality of life. To address this issue, cartilage tissue engineering approaches are currently in development. Bone marrow-derived mesenchymal progenitor cells (MPCs) hold much promise as an alternative cell source for cartilage tissue engineering. While previous studies have established that MPCs from humans and multiple other species undergo in vitro chondrogenic differentiation, additional research is needed to define condit

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Mamdoh, Magdy Nashed Gaid Mariam [Verfasser], and Ludger [Akademischer Betreuer] Beerhues. "Benzoic acid metabolism in Sorbus aucuparia cell suspension cultures / Mariam Mamdoh Magdy Nashed Gaid ; Betreuer: Ludger Beerhues." Braunschweig : Technische Universität Braunschweig, 2010. http://d-nb.info/1175827193/34.

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Trexler, Melody May. "A cyclical semi-continuous process for production of heterologous proteins in metabolically regulated plant cell suspension cultures /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2003. http://uclibs.org/PID/11984.

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Gouws, Anton. "Optimum temperatures for colour development in apples." Thesis, Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/5164.

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Thesis (MScAgric (Horticulture))--University of Stellenbosch, 2010.<br>ENGLISH ABSTRACT: Peel colour is an important quality factor in the production of bi-coloured apple fruit. Most markets set minimum requirements for red colour coverage. Fruit that do not meet these requirements are downgraded and has a major impact on the profitability of apple production in South Africa. South African apple production areas are amongst the warmest in the world. Since anthocyanin accumulation requires induction at low temperature and synthesis require mild temperatures, experiments were conducted to inv

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Velez, Suberbie L. "Characterisation of the bioreactor environment and its effect on mammalian cell performance in suspension culture during antibody production." Thesis, University College London (University of London), 2013. http://discovery.ucl.ac.uk/1410199/.

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The production of therapeutic proteins with complex post-translational modifications is typically performed with suspension adapted mammalian cells, in stirred tank bioreactors (STRs). In this environment cells are exposed to hydrodynamic forces derived from both agitation and aeration. An improved understanding of these forces and the resulting cellular response is therefore critical. This thesis has characterised the hydrodynamic conditions within a STR using computational fluid dynamics and investigated the lethal and sub-lethal effects of this environment on CHO cells during the production

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Lai, Ying-Hui, and 賴穎慧. "Production of the mGMCSF in rice cell suspension culture." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/19499568135528010150.

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碩士<br>國立中央大學<br>生命科學研究所<br>97<br>After the production of insulin by E. coli in 1970s, more and more therapeutical proteins were produced by different organisms, such as microorganism, insect and mammal. In the past few years, the plant expression system had been used to produce many kinds of recombinant proteins because of low cost, safety and post-translational modification. The rice cell production system is one of the most efficient plant expression systems. To improve the yield of the rice cell expression system, different signal peptides including αAmy3, CIN1 and 33kD have fused to the N-

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Cheng, Yuan-Shun, and 振芫舜. "Fuel Cell Hybrid Scooter Suspension Design and Vibration Analysis." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/34236347903018266342.

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碩士<br>國立臺灣大學<br>機械工程學研究所<br>97<br>Fuel cell system is an important part to extend mileage and reduce recharging time for electrical vehicle. However, there are some limits under operation; the vibration tolerance is much lower than internal combustion engine. Although fuel cell stack operates with few moving parts and almost no vibration, and the vibration from road could still cause damage to the fuel cell stack. In this study, a combination of fuel cell hybrid scooter vibrating simulation analysis and suspension system adjustments were proposed to reduce the road impact acceleration on fuel

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Lin, Jin-Lan, and 林錦蘭. "Cell suspension culture of panax ginseng C. A. meyer." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/21117958435956403079.

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碩士<br>國立臺灣大學<br>園藝學研究所<br>84<br>Embryogenic cell suspension culture was established from long -time cultured root drieved embryogenic callus. Embryogenic callus cultured in modified MS liquid medium with 5% coconut milk and 0.5 g/l casein hydrolysate produced shoots.The shooting callus transfered to B5 solid medium which is contained 1 ppm GA and 1 ppm BA produced green plants with normal shoots and roots. Transfer the callus to MS solid me

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Chin, Chou Yun, and 周昀瑾. "Establishment of a rice tPA-transgenic cell suspension culture system." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/37555902184986500299.

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碩士<br>輔仁大學<br>生物學系<br>89<br>tPA( tissue-type plasminogen activator) protein, known to be able to activate and convert plasminogen into plasmin in blood and functionally dissolve blood clots, has been succefully used to treat heal acute ischemic stroke and myocardial infraction clinically. Commercially available tPA is commonly recombinant tPA lacking glycosylation. Completely glycosylation will , however, enhance tPA’s bioactivity up to 40-50% according to the research by Sinniger et al. This research work has been aimed to use transgenic plant which has a glycosylation machinery comparable to

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何佳靜. "studies on callus and suspension cell culture of Ginkgo biloba." Thesis, 1998. http://ndltd.ncl.edu.tw/handle/52722092894223431275.

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碩士<br>國立東華大學<br>生物技術研究所<br>87<br>Ginkgo (Ginkgo biloba), a species of ginkgoaceae, was first recorded in Zea-Ung-Pen-Ts''ao-Ching. Its fruit, one of the Chinese medicinal herbs, was used mainly for treating cough. The purpose of this study was to establish the suspension culture of Ginkgo biloba for producing its secondary metabolites, such as ginkgolide B (GKB) and quercetin. Cell suspension culture was established from the leaves- derived callus. The culture conditions for both cell growth and secondary metabolites production were then evaluated. It was found that MS basal medium

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Nims, Nathan Ezekiel. "The regulation of Taxol production in Taxus suspension cell culture." 2008. https://scholarworks.umass.edu/dissertations/AAI3336965.

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Plant-derived specialized metabolites are used as pharmaceuticals, dyes and fragrances. Due to the complex nature of these compounds, the continued dependence on plants to synthesize these products has persisted. Many of these products are found at low abundance in plants, so increasing the production of these compounds through metabolic engineering is desirable. An example of a plant-derived pharmaceutical found at low abundance is taxol (Taxol® - Bristol-Myers Squib). Taxol is an anti-cancer agent that is in high demand. Plants in the genus Taxus produce Taxol. Taxus cell suspension cultures

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Liu, Chung-Min, and 劉重民. "Studies on Callus nad Cell Suspension Culture of Bupleurum marginatum Willd." Thesis, 1997. http://ndltd.ncl.edu.tw/handle/24614137613480253510.

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吳國隆. "Production of L-DOPA via cell suspension cultures of Stizolobium hassjoo." Thesis, 1992. http://ndltd.ncl.edu.tw/handle/43575671641576336047.

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Liu, Shi-Hua, and 劉士華. "Studies on cell suspension culture and somatic embryogenesisi of Musa formosana." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/44187355552827515211.

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碩士<br>國立臺灣大學<br>園藝學研究所<br>97<br>Summary The young male inflorescence of Taiwan native diploid banana (Musa formosana) was used as explant, and cultured in modified MS medium supplemented with complex auxin including IAA 1 mg/L, NAA 1 mg/L, and 2,4-D 1 mg/L and Picloram 3 mg/L suitable for callus formation. The top male hand portions ranged from the 13th to 17th sections were capable to give rise 64-82% callus formation on the medium as above. The zygote embryos were aptly to generate embryogenic callus on MS medium added with NAA 1 mg/L, IAA 1 mg/L combined Picloram 1 mg/L or Dicamba 1 mg/L in

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Liao, Hui-fan, and 廖慧帆. "Application of light weight PLGA microcarrier to adhesive cell suspension culture." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/56041819437618535983.

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碩士<br>大同大學<br>生物工程學系(所)<br>96<br>Glycosylation and post-translational modification of recombinant proteins are the unique feature of mammalian cell system. However, the productivity of recombinant protein by mammalian cells is much lower than that of microorganisms. Chinese hamster ovary cell line is the most popular mammalian host for the commercial production of therapeutic proteins. We used Chinese hamster ovary cells (5/9 M alpha 3-18, BCRC 60185, CHO cells) to express macrophage colony-stimulating factor (M-CSF), which can stimulate macrophage proliferaction, differenciation and su

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QUE, FU-XIN, and 闕甫伈. "tudies on the cell suspension culture of angelica sinensis (Oliv.)Diel." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/55731045186869173620.

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Liu, Chi-Han, and 劉季函. "Suspension cell culture and berberine production of Phellodendron wislonii Hayata＆Kanehira." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/69274716429124656030.

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碩士<br>中臺科技大學<br>藥物科技研究所<br>99<br>Berberine is an important medicine used for stomach and intestinal illness. Whang-Po( Phellodendro wislonii Hayata et Kanehira ), a deciduous tree of Rutaceae, was first recorded in Shen- Nung-Pen-Ts,ao- Ching under the inferior category and it was listed in Pen-Ts,ao- Gang–Mu as superior medicine the middle woody category. Whang-Po is used for curing fevers, inflammation, etc. Taiwan Whang-Po( P. wilsonii Hayata et Kanehira) , a native plant in Taiwan, is an excellent source of Whang-Po material because of high berberine content. Taiwan Whang-Po must be grown

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Han, Shih-Cheng, and 韓士晟. "Studies on the cell suspension culture ofSaussurea involucrata Karelin et Kirilov." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/22xv5t.

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碩士<br>國立中興大學<br>農藝學系所<br>106<br>Saussurea involucrate is one of the most important medicinal plants in China. It has used in the Chinese system of traditional medicine for the treatment of many disease such as arthritis, hypertension, cancer, gynecological diseases, etc. Owing to over-exploitation of the wild plants for commercial purposes, ecological destruction of the natural habits, and the difficulty of cultivation. The species has become endangered. Therefore, we need a more efficient way to propagate this plant. Plant tissue culture is a technique for rapid multiplication of the whole pl

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Chi-Ming, Yu, and 余齊明. "Prediction of Operational Strategy for Plant Cell Suspension Culture by Neural Network." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/01383783623790850133.

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碩士<br>國立臺灣大學<br>化學工程學研究所<br>87<br>Artificial Neural networks were employed to simulate the cell growth and secondary metabolite production of plant cell culture system. Using experimental data of plant suspension cell culture, the optimal operation condition and the parameters for scaling up the bioreactor were investigated. Feed forward back propagation artificial networks was adopted for network structure. One or two hidden layers which were connected with the complexity of the input data was tried. Sigmoid type transfer function was implemented to the hidden layer while pure line

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