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Artykuły w czasopismach na temat „Centrifugation technique”

Autor: Grafiati

Data publikacji: 2 czerwca 2025

Data aktualizacji: 31 lipca 2025

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1

Maharani, Eva Ayu, and Dewi Astuti. "Comparison of Single Centrifugation, Double Centrifugation and Turn down-Turn up Techniques for Platelet-Rich Plasma Quality." Althea Medical Journal 9, no. 3 (2022): 180–84. http://dx.doi.org/10.15850/amj.v9n3.2628.

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Background: Platelet-rich plasma (PRP) is a new concept used in medical world, especially for wound healing. The main process that affects the PRP quality is the centrifugation process. This study aimed to assess the PRP separation process and determine the best technique of various centrifugation process.Methods: This experimental study used acid citrate dextrose (ACD) blood taken from 11 healthy respondents and compared three-techniques including the single centrifugation (SC), the double centrifugation (DC), and the double centrifugation turn down - turn up (DC-TDTU) techniques. The quality

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Walker, Randall C. "Lysis-Centrifugation Blood Culture Technique." Archives of Internal Medicine 146, no. 12 (1986): 2341. http://dx.doi.org/10.1001/archinte.1986.00360240055010.

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Novia, Diana, and Binarwan Halim. "#302 : Shorter Centrifugation Time Leads to Better Sperm Quality in Swim-Up Processing Technique." Fertility & Reproduction 05, no. 04 (2023): 700. http://dx.doi.org/10.1142/s2661318223744120.

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Background and Aims: Centrifugation is one of the many factors that cause sperm DNA damage in IVF semen preparation. This can occur due to the formation of reactive oxygen species (ROS) that are uncontrollable, so that the quality of sperm DNA can be damaged. This study aims to compare sperm quality results with swim-up technique under centrifugation times of 5 and 10 minutes. Method: This study involved 50 patients at the Halim Fertility Center clinic from June 2020 to October 2020. The samples obtained were processed using the swim-up method. Samples were divided into 3 groups, namely contro

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Fallatah, Majed Hashem, Aaied Falah Al Otaibi, Randa Rajallah Aljohani, Waad Jamal Ghabban, and Alaa Ali Alshehri. "Evaluation of Centrifugation Technique and the Effect of Centrifugation Condition on the Laboratory Samples." JOURNAL OF HEALTHCARE SCIENCES 04, no. 12 (2024): 1003–9. https://doi.org/10.52533/johs.2024.41248.

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Centrifugation is a critical laboratory technique used to separate components within a mixture based on density differences. The efficiency and reliability of this process depend on the precise control of variables such as speed, time, temperature, and rotor design. Improper centrifugation settings can compromise sample integrity, leading to analytical inaccuracies. High-speed centrifugation, while effective for rapid separation, often imposes mechanical stress on samples, resulting in hemolysis or cellular damage. Time duration is equally significant, as insufficient centrifugation can cause

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Taglioretti, V., N. Sardella, and M. Fugassa. "Effectiveness of coproscopic concentration techniques." Helminthologia 51, no. 3 (2014): 210–14. http://dx.doi.org/10.2478/s11687-014-0231-x.

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AbstractThe aim of the present study was to compare the effectiveness of the concentration techniques of flotation-centrifugation with zinc chloride (FZn) (δ = 1.45) with the spontaneous sedimentation (SSed) and the sucrose flotation-centrifugation (FSuc) (δ = 1.2) to recuperate gastrointestinal parasites from camelid fecal samples. The technique with more positive results for the detection of Nematodirus sp., Trichuris sp., strongyle-type eggs and Eimeria macusaniensis oocysts was the FZn. For Trichuris sp. and Eimeria macusaniensis, the higher coverglass counts were detected by FZn procedure

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Lebl, Michal. "Centrifugation Based Automated Synthesis Technologies." JALA: Journal of the Association for Laboratory Automation 8, no. 3 (2003): 30–35. http://dx.doi.org/10.1016/s1535-5535-04-00267-9.

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Centrifugation is a powerful method for solid-liquid separation. It can be applied in numerous ways to simplify solid phase synthetic procedures. At the same time, centrifugation is the only totally parallel technique which can be scaled up for processing volume or number of simultaneously run reactions, without the limitation of overpressure or vacuum-driven filtration-based systems. We have developed synthesizers based on the power of centrifugation — peptide and small organic molecule synthesizers utilizing cotton as the synthetic substrate and inclusion volume chemistry, synthesizers for a

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7

Matas, C., J. Gadea, and G. Decuadro-Hansen. "89 EVALUATION OF A CUSHIONED CENTRIFUGATION TECHNIQUE FOR PROCESSING BOAR SEMEN FOR FREEZING." Reproduction, Fertility and Development 17, no. 2 (2005): 195. http://dx.doi.org/10.1071/rdv17n2ab89.

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Boar semen freezing procedures include the use of centrifugation to concentrate sperm and remove seminal plasma prior to dilution in freezing extender. The centrifugation techniques employed have necessarily been a compromise between the need to recover as many spermatozoa as possible after centrifugation and the damage caused by pelleting the sperm. The use of an inert, dense, and isotonic solution as a cushion in the bottom of the tube leads to the use of higher-speed centrifugation to ensure maximum sperm recovery. However, it is necessary to know the viability and functionality of the samp

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Singhal, Ankita, Sheetal Goenka, and Dimple Kasana. "Revolutionizing Sterile Sample Culturing: Cost-Effective Centrifugation Techniques for Research in Resource- Constrained Settings." European Journal of Medical and Health Research 1, no. 1 (2023): 30–33. http://dx.doi.org/10.59324/ejmhr.2023.1(1).05.

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Introduction: The yield (culture positive rate) of sterile body fluids in patients was found to be less than 50%. It has been demonstrated that centrifuging body fluid before culturing increases yield. Using concentrated bacterial samples to enhance culture growth is a more effective alternative. We sought to determine whether sterile fluid culture yield was increased by centrifugation. Methods: Patients whose body fluids were received in sufficient quantity in the microbiology lab were enrolled. Sterile body fluids culture was carried out after centrifugation and by routine conventional inocu

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Singhal, Ankita, Sheetal Goenka, and Dimple Kasana. "Revolutionizing Sterile Sample Culturing: Cost-Effective Centrifugation Techniques for Research in Resource- Constrained Settings." European Journal of Medical and Health Research 1, no. 1 (2023): 30–33. https://doi.org/10.59324/ejmhr.2023.1(1).05.

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Introduction: The yield (culture positive rate) of sterile body fluids in patients was found to be less than 50%. It has been demonstrated that centrifuging body fluid before culturing increases yield. Using concentrated bacterial samples to enhance culture growth is a more effective alternative. We sought to determine whether sterile fluid culture yield was increased by centrifugation. Methods: Patients whose body fluids were received in sufficient quantity in the microbiology lab were enrolled. Sterile body fluids culture was carried out after centrifugation and by routine conventional inocu

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Santana, Tamara, Jorge Moreno, Guillermo Petzold, Roberto Santana, and Guido Sáez-Trautmann. "Evaluation of the Temperature and Time in Centrifugation-Assisted Freeze Concentration." Applied Sciences 10, no. 24 (2020): 9130. http://dx.doi.org/10.3390/app10249130.

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Centrifugation is a technique applied to assist in the freeze concentration of fruit juices and solutions. The aim of this work was to study the influence of the time–temperature parameters on the centrifugation process as a technique applied to assist in the first cycle of the freeze concentration of blueberry juice. A completely randomized 4 × 3 factorial design was performed using temperature and time as the factors, and the response variables included the percentage of concentrate, efficiency and solutes recovered. The results were evaluated using multiple linear regression, random forest

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Yin, X., L. R. Zeph, and G. Stotzky. "A simple method for enumerating bacteriophages in soil." Canadian Journal of Microbiology 43, no. 5 (1997): 461–66. http://dx.doi.org/10.1139/m97-065.

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A plaque technique that uses antibiotic-resistant bacteria growing on antibiotic-containing agar for the assay lawn resulted in significantly better recovery of bacteriophages P1 of Escherichia coli and F116 of Pseudomonas aeruginosa from nonsterile soil than standard membrane filtration or centrifugation techniques. Adsorption of the phages on soil particles appeared to be involved in their recovery and survival in soil.Key words: bacteriophages, soil, antibiotic-resistant bacteria, enumeration, filtration, centrifugation.

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Gadea, J., S. Martínez-Miró, G. Decuadro-Hansen, and C. Matás. "92 EVALUATION OF BOAR SPERM FUNCTIONALITY AFTER A CUSHIONED CENTRIFUGATION TECHNIQUE." Reproduction, Fertility and Development 18, no. 2 (2006): 154. http://dx.doi.org/10.1071/rdv18n2ab92.

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Separation of sperm from seminal plasma is required in most semen freezing procedures. Semen is typically subjected to centrifugation to concentrate sperm into a pellet and allow removal of the seminal plasma prior to dilution in freezing extender. Centrifugation is a relatively effective method to recover sperm, however, the process also causes considerable sperm damage. The use of a dense, inert, and isotonic solution as a cushion in the bottom of the centrifuge tube allows a greater centrifugation speed to be applied and results in greater sperm recovery. The aim of the present work was to

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13

Traynor, L., R. Sutton, J. Riley, FA Crawford, and RM Sade. "Comparing Ultrafiltration and Centrifugation During and After Pediatric Cardiopulmonary Bypass." Journal of ExtraCorporeal Technology 23, no. 3 (1991): 140–51. http://dx.doi.org/10.1051/ject/1991233140.

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Blood processing techniques related to pediatric cases have previously been generalized from adult experiences. Twenty-two pediatric patients (<25 kilograms) undergoing cardiopulmonary bypass (CPB) for various congenital defects were prospectively randomized into an ultrafiltration or centrifugation group. Sixty-one variables were compared. The blood infused from the ultrafiltration technique contained significantly more heparin (p=0.0001) and more plasma-free hemoglobin (PFH) (p=0.0001) levels than the centrifugation blood. Centrifugation patients had a significantly higher hematocrit (p=0

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14

Karina, Karina, Johannes A. Biben, Krista Ekaputri, et al. "Revisiting Fat Graft Harvesting and Processing Technique to Optimize Its Regenerative Potential." Plastic and Reconstructive Surgery - Global Open 13, no. 1 (2025): e6420. https://doi.org/10.1097/gox.0000000000006420.

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Background: The use of fat grafting has expanded to include cell and tissue regeneration, necessitating investigations to ensure the viability of stromal and adipose-derived mesenchymal stem cells (ASCs) within the transferred fat parcels. This study explored the impact of harvesting technique and centrifugation on the viability of stromal cells and ASCs in lipoaspirate. Methods: Fat was harvested from patients undergoing fat grafting using 2 types of liposuction cannula: (A) a 3-mm blunt tip cannula with 3 smooth holes and (B) a 2.4-mm, sharp point port, multihole blunt tip cannula. Fat from

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15

Rey, Marcel, Maximilian J. Uttinger, Wolfgang Peukert, Johannes Walter, and Nicolas Vogel. "Probing particle heteroaggregation using analytical centrifugation." Soft Matter 16, no. 14 (2020): 3407–15. http://dx.doi.org/10.1039/d0sm00026d.

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We study the aggregation of silica particles and poly(N-isopropylacrylamide) microgels by analytical centrifugation. We demonstrate that the technique can yield quantitative information on the formation of defined clusters and large aggregates.

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Liu, Yong De, Hai Lu Wu, Jie Zhang, Dong Jin Wan, and Ji Hong Zhao. "Study of Porcine Blood Decoloration Technique." Advanced Materials Research 518-523 (May 2012): 3980–83. http://dx.doi.org/10.4028/www.scientific.net/amr.518-523.3980.

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Livestock blood is a slaughtering by-product that has potential for animal protein sources, human consumption is limited due to its brown color causing dark hues to food and feed products. The aim of this work is to extract white and palatable protein from red cells of porcine blood. Red cells are hydrolyzed with papain and acidized with Hydrochloric acid, freed heme group is flocculated with Sodium Carboxy Methyl Cellulose (CMC) and separated by centrifugation, supernatant is the decoloring protein solution. The enzyme hydrolysis conditions and flocculation conditions are optimized. The optim

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Katzap, Roberta M., Vany Elisa Pagnussatti, Ana Elizabeth Figueiredo, et al. "Time to Positivity of Bacteria Cultures in Peritoneal Dialysis Fluid: Evaluation of Different Laboratory Techniques." Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis 37, no. 3 (2017): 342–44. http://dx.doi.org/10.3747/pdi.2016.00174.

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Patients with chronic kidney disease on peritoneal dialysis (PD) are susceptible to infections, with peritonitis being the primary cause of dropout. Peritoneal fluid culture is one of the essential elements for proper diagnosis and peritonitis treatment. The aim of this study was to compare the time required to obtain a positive culture using different laboratory methods. An in vitro cross-sectional study was conducted comparing different techniques for preparation and culture of bacteria in peritoneal fluid. The research was carried out with 21 sterile dialysis bags and 21 PD bags containing

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Denardin, Norimar D'Ávila, and Vanessa Andréia Agostini. "Detection and quantification of Xanthomonas axonopodis pv. phaseoli and its variant fuscans in common bean seeds." Journal of Seed Science 35, no. 4 (2013): 428–34. http://dx.doi.org/10.1590/s2317-15372013000400003.

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The common bacterial blight of common beans (CBCF), a disease caused by Xanthomonas axonopodis pv. phaseoli (Xap) and Xanthomonas axonopodis pv. phaseoli var. fuscans (Xapf), significantly reduces grain yield and seed quality. Because this bacterium is mainly disseminated through infected seeds, efficient detection of Xap and Xapf is important to assure the productivity and quality of the crop. In this study, various techniques that included different extraction techniques (two different incubation times, with and without centrifugation) and five culture media (Kado 523, GYCA, MXP, NSA, and PT

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Massing, Ulrich, Sanja Cicko, and Vittorio Ziroli. "Dual asymmetric centrifugation (DAC)—A new technique for liposome preparation." Journal of Controlled Release 125, no. 1 (2008): 16–24. http://dx.doi.org/10.1016/j.jconrel.2007.09.010.

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Nelissen, Xavier, Séverine Licciardi, Christophe Nizet, Emmanuel Delay, and Régis Roche. "Comparative Analysis of a New Automatic System and Four Existing Techniques for Autologous Fat Grafting." Plastic and Reconstructive Surgery - Global Open 11, no. 10 (2023): e5349. http://dx.doi.org/10.1097/gox.0000000000005349.

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Background: Autologous fat grafting is increasingly used worldwide and is a very attractive technique in many ways. However, treatment duration and postinjection tissue resorption remain problematic elements, which are largely related to the preparation method used. Moreover, few scientific studies objectively compare different fat preparation methods. This study analyzes the efficiency and quality of lipoaspirates prepared with a new filtration/centrifugation system (Adipure) in comparison with several existing techniques. Methods: Patient lipoaspirates were processed by five different techni

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Chandler, Ekta, Naveen Kakkar, and Rupinder Kaur. "Comparison of Rapid Centrifugation Technique with Conventional Centrifugation for Prothrombin Time (PT) and Activated Partial Thromboplastin Time (APTT) Testing." Indian Journal of Hematology and Blood Transfusion 35, no. 1 (2018): 161–66. http://dx.doi.org/10.1007/s12288-018-0983-4.

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Gebre-Selassie, Solomon. "Evaluation of the Concentration Sputum Smear Technique for the Laboratory Diagnosis of Pulmonary Tuberculosis." Tropical Doctor 33, no. 3 (2003): 160–62. http://dx.doi.org/10.1177/004947550303300313.

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The microbiological diagnosis of pulmonary tuberculosis (PTB) plays a key role in routine and Tuberculosis (TB) Control Programmes in developing countries. Concentration of acid-fast bacilli (AFB) in clinical specimens is an important step in the laboratory diagnosis of mycobacterial diseases. Microscopy of smears of sputum by direct and after mechanical sedimentation and centrifugation methods followed by treatment with 5% sodium hypochlorite (NaOCl) solution for concentration of the organisms were compared and evaluated. The rate of recovery of AFB from sputum was 8.5%, 25.5% and 38.0% for d

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Haraguchi, Yuji, Akiyuki Hasegawa, Katsuhisa Matsuura, et al. "Three-Dimensional Human Cardiac Tissue Engineered by Centrifugation of Stacked Cell Sheets and Cross-Sectional Observation of Its Synchronous Beatings by Optical Coherence Tomography." BioMed Research International 2017 (2017): 1–8. http://dx.doi.org/10.1155/2017/5341702.

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Three-dimensional (3D) tissues are engineered by stacking cell sheets, and these tissues have been applied in clinical regenerative therapies. The optimal fabrication technique of 3D human tissues and the real-time observation system for these tissues are important in tissue engineering, regenerative medicine, cardiac physiology, and the safety testing of candidate chemicals. In this study, for aiming the clinical application, 3D human cardiac tissues were rapidly fabricated by human induced pluripotent stem (iPS) cell-derived cardiac cell sheets with centrifugation, and the structures and bea

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Bisiau, Christian, Paula Moffett, James Graham, and Patrick McCue. "Comparison of Nanoparticles and Single-Layer Centrifugation for Separation of Dead from Live Stallion Spermatozoa." Veterinary Sciences 11, no. 7 (2024): 307. http://dx.doi.org/10.3390/vetsci11070307.

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The goal of this study was to compare the efficacy of coated iron-core nanoparticles and single-layer centrifugation for separation of dead from live stallion spermatozoa. Our hypothesis was that nanoparticles would bind to dead sperm and allow for separation from live sperm using a magnet, resulting in a population of spermatozoa with a high percentage of total and progressive motility. Treatment Group 1 was an untreated control. Treatment Group 2 (nanoparticles, NP) utilized sperm incubated with nanoparticles followed by application of a magnet to remove dead sperm adhered to the coated nano

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Webb, Gary W., and M. M. Dean. "Effect of Centrifugation Technique on Post-storage Characteristics of Stallion Spermatozoa." Journal of Equine Veterinary Science 29, no. 9 (2009): 675–80. http://dx.doi.org/10.1016/j.jevs.2009.07.016.

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Walker, R. C. "Lysis-centrifugation blood culture technique. Clinical impact in Staphylococcus aureus bacteremia." Archives of Internal Medicine 146, no. 12 (1986): 2341–43. http://dx.doi.org/10.1001/archinte.146.12.2341.

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Al-Timimi, Ihsan H. S. "Selection of male goat embryos in vitro by using swim-up of epididymal spermatozoa." Iraqi Journal of Veterinary Medicine 42, no. 1 (2018): 79–86. http://dx.doi.org/10.30539/iraqijvm.v42i1.35.

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The main objectives of this study is the separation of X from Y bearing epididymal spermatozoa of local buck by swim-up, and the use of this spermatozoa for in vitro fertilization to determine the percentage of produced male and female embryos. The sex of produced embryo was identified by polymerase chain reaction. Testis of the local buck were obtained from Al-Shu'alah abattoir and the epididymal spermatozoa were harvested from the cauda by and submitted to in vitro maturation prior to separation of X from Y bearing spermatozoa and prior to their use for in vitro fertilization. For the separa

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Hu, Qin, Xiaojuan Gong, Lizhen Liu, and Martin M. F. Choi. "Characterization and Analytical Separation of Fluorescent Carbon Nanodots." Journal of Nanomaterials 2017 (2017): 1–23. http://dx.doi.org/10.1155/2017/1804178.

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Carbon nanodots (C-dots) are recently discovered fluorescent carbon nanoparticles with typical sizes of <10 nm. The C-dots have been reported to have excellent photophysical and chemical characteristics. In recent years, the advances in the development and improvement in C-dots synthesis, characterization, and applications are burgeoning. In this review, we introduce the most commonly used techniques for the characterization of C-dots. The characterization techniques for C-dots are briefly classified, described, and illustrated with applied examples. In addition, the analytical separation m

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Yudiwati, Rina, MPBD Pramesti, Agustinus Agustinus, E. Pradana, and Bambang Purwanto. "IMPACT OF PREPARATION USING CONVENTIONAL AND MODIFIED DENSITY GRADIENT CENTRIFUGATION METHODS ON SPERM CONCENTRATION, MOTILITY AND NUMBER OF NORMAL MOTILE SPERM RECOVERY (NMSR)." Folia Medica Indonesiana 53, no. 3 (2017): 196. http://dx.doi.org/10.20473/fmi.v53i3.6447.

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Some preparation techniques, either conventional or advanced, have been provided. Advanced technique may overcome the limitations of conventional techniques. Recently, not all fertility clinics in Indonesia are able to provide advanced preparation techniques. Some techniques require expensive equipments and can only be used for intracytoplasmic sperm injection (ICSI). Some fertility clinics use a modified procedure, namely the combination of density gradient centrifugation with a swim-up method for the preparation of the sperm to be used in ART. This study aimed to determine whether the modifi

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Büscher, Philippe, Dieudonné Mumba Ngoyi, Jacques Kaboré, et al. "Improved Models of Mini Anion Exchange Centrifugation Technique (mAECT) and Modified Single Centrifugation (MSC) for Sleeping Sickness Diagnosis and Staging." PLoS Neglected Tropical Diseases 3, no. 11 (2009): e471. http://dx.doi.org/10.1371/journal.pntd.0000471.

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Chetverikov, Sergiy, and Dmitro Atanasov. "ORIGINAL EFFECTIVE, SAFE TECHNIQUE OF OBTAINING PLATELET RICH PLASMA BY CENTRIFUGATION OF THE BLOOD PLASMA IN MODIFIED SYRINGE CONTAINERS." EUREKA: Health Sciences 1 (January 31, 2019): 3–9. http://dx.doi.org/10.21303/2504-5679.2019.00844.

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The aim: to develop, substantiate an effective and safe technology for producing PRP (platelet rich plasma). To quantify the substrate based on the recommended centrifugation protocols. Materials and methods: the effectiveness of the original harvesting protocol was evaluated by quantifying the number of platelets. The proposed technique is formed basing on the basic principles of double centrifugation of whole blood in test tubes with anticoagulant, separation with the release of a plasma layer with a high content of platelets. The centrifuging mode for quantifying the effectiveness of the su

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Sergiy, Chetverikov, and Atanasov Dmitro. "ORIGINAL EFFECTIVE, SAFE TECHNIQUE OF OBTAINING PLATELET RICH PLASMA BY CENTRIFUGATION OF THE BLOOD PLASMA IN MODIFIED SYRINGE CONTAINERS." EUREKA: Health Sciences, no. 2 (March 31, 2019): 3–9. https://doi.org/10.21303/2504-5679.2019.00844.

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<strong>The aim:</strong> to develop, substantiate an effective and safe technology for producing PRP (platelet rich plasma). To quantify the substrate based on the recommended centrifugation protocols. <strong>Materials and methods:</strong> the effectiveness of the original harvesting protocol was evaluated by quantifying the number of platelets. The proposed technique is formed basing on the basic principles of double centrifugation of whole blood in test tubes with anticoagulant, separation with the release of a plasma layer with a high content of platelets. The centrifuging mode

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Bonilla-Gutiérrez, Andres Felipe, Catherine Aragón-Urrego, and Omar Leonardo Aristizábal-Páez. "Protocolo para la obtención de un Concentrado Autólogo de Plaquetas en conejos: estudio piloto." Revista de la Facultad de Medicina Veterinaria y de Zootecnia 64, no. 1 (2017): 24–31. http://dx.doi.org/10.15446/rfmvz.v64n1.65813.

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One of the main difficulties presented in surgical practice, is the restoration of the integrity of the tissues that are affected by the pathology or the surgical technique performed for its resolution. Currently, techniques and treatments are developed focused to improve scarring processes tissue and promote tissue regeneration; one of them is Autologous Platelets Concentrate (APC). The aim of the present study was evaluate the blood collection and processing technique for obtaining APC in a lagomorph model. New Zealand male rabbits (n=10), with weights ranging from 3 to 3.8 kg, were used as

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Sinha, K., U. Tendolkar, and M. Mathur. "Comparison of Conventional Broth Blood Culture Technique and Manual Lysis Centrifugation Technique for Detection of Fungemia." Indian Journal of Medical Microbiology 27, no. 1 (2009): 79–80. http://dx.doi.org/10.1016/s0255-0857(21)01766-7.

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Chetverikov, Sergiy, and Dmitro Atanasov. "EVALUATION OF CENTRIFUGING REGIMES FOR THE PURPOSE OF OPTIMIZING THE PLATELET RICH PLASMA HARVESTING PROTOCOL." EUREKA: Health Sciences 2 (March 31, 2019): 11–18. http://dx.doi.org/10.21303/2504-5679.2019.00881.

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Aim: Based on the classical principles, to determine the optimal conditions for centrifugation, PRP harvesing (platelet-rich plasma). To conduct a quantitative assessment of the substrate obtained under different conditions of centrifugation. Materials and methods. Based on the basic principles of obtaining platelet-rich plasma (PRP) by centrifuging in containers with an anticoagulant followed by phase separation to obtain the final substrate, the efficiency of the technique under the conditions of single and double centrifugation as well as under different conditions of acceleration and centr

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GUTIERREZ, CARLOS, JUAN A. CORBERA, FRANCISCO DORESTE, and PHILIPPE BÜSCHER. "Use of the Miniature Anion Exchange Centrifugation Technique to IsolateTrypanosoma evansifrom Goats." Annals of the New York Academy of Sciences 1026, no. 1 (2004): 149–51. http://dx.doi.org/10.1196/annals.1307.020.

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Proudman, C., and G. Edwards. "Validation of a centrifugation/flotation technique for the diagnosis of equine cestodiasis." Veterinary Record 131, no. 4 (1992): 71–72. http://dx.doi.org/10.1136/vr.131.4.71.

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van der Vegt, S. G. L., A. M. Th Ruben, J. M. Werre, et al. "Counterflow centrifugation of red cell populations: a cell age related separation technique." British Journal of Haematology 61, no. 3 (1985): 393–403. http://dx.doi.org/10.1111/j.1365-2141.1985.tb02843.x.

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Meruane, Manuel, Alison Ford, Alejandro Swett, and Alvaro Ibarra. "The Impact of Liposuction Technique, Centrifugation and Freezing in Adipose Tissue Viability." Plastic and Reconstructive Surgery 134 (October 2014): 53–54. http://dx.doi.org/10.1097/01.prs.0000455393.68681.6a.

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Fournier, P. E., M. Drancourt, H. Lepidi, M. J. Gevaudan, and D. Raoult. "Isolation of Mycobacteria from Clinical Samples Using the Centrifugation-Shell Vial Technique." European Journal of Clinical Microbiology & Infectious Diseases 19, no. 1 (2000): 69–70. http://dx.doi.org/10.1007/s100960050015.

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Hopkins, D. W., S. J. Macnaughton, and A. G. O'Donnell. "A dispersion and differential centrifugation technique for representatively sampling microorganisms from soil." Soil Biology and Biochemistry 23, no. 3 (1991): 217–25. http://dx.doi.org/10.1016/0038-0717(91)90055-o.

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Buhler, Douglas D., and Bruce D. Maxwell. "Seed Separation and Enumeration from Soil Using K2Co3-Centrifugation and Image Analysis." Weed Science 41, no. 2 (1993): 298–302. http://dx.doi.org/10.1017/s0043174500076207.

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This research presents a modified floatation method to extract weed seed from soil samples and an automated imaging technique to enumerate seed following extraction. Rapid floatation of weed seed from soil in a K2CO3solution was facilitated by centrifugation. High recovery of seed from soil samples was accomplished in a relatively short time. Recovery of seed of giant foxtail and velvetleaf from a silt loam soil was near 100% and recovery of redroot pigweed was 86%. Germination of common lambsquarters, giant foxtail, and velvetleaf seed was reduced by exposure to K2CO3under certain conditions.

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Harnnoi, T., A. Wijit, N. Morakote, V. Pipitgool, and W. Maleewong. "Specific gravity of Opisthorchis viverrini eggs." Journal of Helminthology 72, no. 4 (1998): 359–61. http://dx.doi.org/10.1017/s0022149x00016746.

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AbstractThe specific gravity of the eggs of the liver fluke Opisthorchis viverrini was determined using a sucrose gradient centrifugation and found to range from 1.2713 to 1.3043. The peak egg count was located at the sucrose fraction with a specific gravity of 1.2814. An attempt to float eggs in saturated sodium nitrate solution, sp.gr. 1.4, failed. Examination of human stool specimens for O. viverrini eggs by simple flotation in saturated sodium nitrate solution and the formol-ether sedimentation technique revealed that the flotation technique was not as efficient as the sedimentation techni

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Sergiy, Chetverikov, and Atanasov Dmitro. "EVALUATION OF CENTRIFUGING REGIMES FOR THE PURPOSE OF OPTIMIZING THE PLATELET RICH PLASMA HARVESTING PROTOCOL." EUREKA: Health Sciences, no. 2 (March 31, 2019): 11–18. https://doi.org/10.21303/2504-5679.2019.00881.

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<strong>Aim: </strong>Based on the classical principles, to determine the optimal conditions for centrifugation, PRP harvesing (platelet-rich plasma). To conduct a quantitative assessment of the substrate obtained under different conditions of centrifugation. <strong>Materials and methods. </strong>Based on the basic principles of obtaining platelet-rich plasma (PRP) by centrifuging in containers with an anticoagulant followed by phase separation to obtain the final substrate, the efficiency of the technique under the conditions of single and double centrifugation as well as under di

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Aukland, K. "Distribution volumes and macromolecular mobility in rat tail tendon interstitium." American Journal of Physiology-Heart and Circulatory Physiology 260, no. 2 (1991): H409—H419. http://dx.doi.org/10.1152/ajpheart.1991.260.2.h409.

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This study explores a centrifugation technique for estimating interstitial fluid composition and macromolecular transport. Rat tail tendon supported by a nylon net was subjected to three consecutive 15-min centrifugations at 3,000, 6,000, and 14,000 revolutions per minute (rpm) or 3, 10, and 20 min at 6,000 rpm. Colloid osmotic pressure (COP) and concentrations of albumin, total protein, and hyaluronan in subsequent centrifugate fell as exponential functions of accumulated centrifuged volume, reaching 10-30% of initial level at an accumulated volume corresponding to 8% of tendon volume. Interc

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Lamaalla, Y., L. Idelkheir, Z. Alami, et al. "Autologous Fat Grafting for Facial Volume Restoration in Parry-Romberg Syndrome: A Retrospective Study of 7 Cases." Scholars Journal of Medical Case Reports 13, no. 05 (2025): 832–36. https://doi.org/10.36347/sjmcr.2025.v13i05.013.

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Background: Parry-Romberg syndrome (PRS) is a rare craniofacial disorder characterized by progressive hemifacial atrophy. While autologous fat grafting (AFG) has emerged as a promising treatment, long-term volumetric outcomes remain poorly characterized. Methods: This retrospective study analyzed 7 PRS patients (6F:1M; mean age 22±7.2 years) treated between 2021-2024. Fat was harvested via Coleman technique, processed by centrifugation (3000 rpm × 3 min), and injected in three anatomical layers Results: Mean volume retention was 62.3±8.7% at 12 months (p<0.01). Adipocyte viability correlate

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Mailyan, Levon R., Sergey A. Stel’makh, Evgenii M. Shcherban’, et al. "Investigation of Integral and Differential Characteristics of Variatropic Structure Heavy Concretes by Ultrasonic Methods." Applied Sciences 11, no. 8 (2021): 3591. http://dx.doi.org/10.3390/app11083591.

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The article develops methods and methodology for experimental studies of centrifuged and vibro-centrifuged concrete products of annular cross-section. They assess the real variatropy of the structure and confirm the correctness of the accepted research. An original technique for experimental studies of the variatropy of the cross-sections of vibrated, centrifuged and vibro-centrifuged concretes is proposed to determine their integral (common) and differential (differing in layers) strength and strain characteristics and deformation diagrams. It has been proved that with vibro-centrifugation it

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Azcue, Jose M., and Fernando Rosa. "Effects of Sampling Technique on the Determination of Major and Trace Elements on Sediment Pore Water." Water Quality Research Journal 31, no. 4 (1996): 709–24. http://dx.doi.org/10.2166/wqrj.1996.038.

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Abstract Pore water samples obtained by squeezing, centrifuging followed by filtration, and in situ dialysis were compared. The effects of the three methods on the measured concentrations of eight elements (Ca, Fe, K, Mg, Mn, Na, Si and Sr) were studied. Iron and Mn proved to be extremely sensitive to oxygen exposure. Immediate centrifugation followed by filtration under nitrogen yield very similar results for almost all the elements as the in situ dialysis technique. The squeezing technique was the most susceptible to handling variables such as pressure-, oxygen- and temperature-related chang

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Koehler, Jonas K., Stefanie Schmager, Valentin Bender, Denise Steiner, and Ulrich Massing. "Preparation of Nanosized Pharmaceutical Formulations by Dual Centrifugation." Pharmaceuticals 16, no. 11 (2023): 1519. http://dx.doi.org/10.3390/ph16111519.

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Dual centrifugation (DC) is an innovative in-vial homogenization and in-vial nanomilling technique that has been in use for the preparation of liposomes for more than one decade. Since then, DC has continuously been developed for preparing various liposomes and other lipid nanoparticles including emulsions and solid lipid nanoparticles (SLNs) as well as polymersomes and nanocrystals. Improvements in equipment technology have been achieved over the past decade, so that DC is now on its way to becoming the quasi-standard for the simple, fast, and aseptic production of lipid nanoparticles and nan

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Niwa, Rieko, Shigenobu Yoshida, Naruto Furuya, Kenichi Tsuchiya, and Seiya Tsushima. "Method for simple and rapid enumeration of total epiphytic bacteria in the washing solution of rice plants." Canadian Journal of Microbiology 57, no. 1 (2011): 62–67. http://dx.doi.org/10.1139/w10-101.

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The phyllosphere is one of the most common habitats for terrestrial bacteria. However, little is known about the populations of bacteria, including unculturable bacteria, that thrive on plant surfaces. Here, we developed a fluorescent nuclear staining technique to easily and rapidly observe and enumerate populations of total and living epiphytic bacteria, with particular emphasis on the concentration by centrifugation and fixation of the epiphytic bacteria. An investigation on the optimal conditions for centrifugation and fixation revealed that centrifugation at 20 400g for 2 min and fixation

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