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Rozprawy doktorskie na temat „Corneal endothelium”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 25 lipca 2025

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1

Sheng, Huan. "Factors affecting corneal endothelial morphology." Columbus, Ohio : Ohio State University, 2006. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1141395542.

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McGowan, Sara L. "Stem cell markers in the posterior limbus and cornea." Thesis, Birmingham, Ala. : University of Alabama at Birmingham, 2007. https://www.mhsl.uab.edu/dt/2007r/mcgowan.pdf.

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Arancibia, Carcamo Carolina Virgina. "Class II MHC on corneal endothelium : implications for corneal transplantation." Thesis, Imperial College London, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.395028.

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Parker, Douglas George Anthony, and park0290@flinders edu au. "Lentivirus-mediated gene expression in corneal endothelium." Flinders University. Medicine, 2008. http://catalogue.flinders.edu.au./local/adt/public/adt-SFU20081204.094431.

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Modulation of corneal transplant rejection using gene therapy shows promise in experimental models but the most appropriate vector for gene transfer is yet to be determined. The overarching aim of the thesis was to evaluate the potential of a lentiviral vector for use in human corneal transplantation. Specific aims were: (i) to assess the ability of an HIV-1-based lentiviral vector to mediate expression of the enhanced yellow fluorescent protein (eYFP), and a model secreted protein interleukin-10 (IL10), in ovine and human corneal endothelium; and (ii) to examine the influence of lentivirus-me

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Martins, Luís Carlos [UNESP]. "Avaliação ultra-estrutural do endotélio corneal de ratos normais e de diabéticos aloxânicos." Universidade Estadual Paulista (UNESP), 2002. http://hdl.handle.net/11449/88910.

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Made available in DSpace on 2014-06-11T19:23:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2002Bitstream added on 2014-06-13T19:09:41Z : No. of bitstreams: 1 martins_lc_me_botfm.pdf: 1919468 bytes, checksum: d4a42d395d3517a07ebafa18c26a4e02 (MD5)<br>O objetivo do estudo foi avaliar a influência do diabetes experimental sobre a ultra-estrutura do endotélio corneal de ratos. O estudo foi prospectivo, utilizando 20 ratos da raça Wistar, com 3 meses de idade, divididos em: grupo controle (GC), contendo 10 ratos, e grupo diabético (GD), contendo 10 ratos. A indução do diabetes foi feita co

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Parekh, Mohit. "Human corneal endothelial cell culture and corneal transplantation." Doctoral thesis, Università degli studi di Padova, 2017. http://hdl.handle.net/11577/3422398.

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Cornea is the front transparent window of the eye which is responsible for optimal and clear vision. Transparency of this tissue is highly inevitable and cannot be compromised. Human cornea is made up of multiple layers out of which the posterior layer ‘endothelium’ is responsible for the transparency of the cornea. Endothelium is a monolayer of cells that allow the ions and solutes to transport from aqueous humour to the cornea and back which in turn maintains the transparency of the cornea by preserving the homeostasis between the anterior and posterior cornea. Earlier, it was observed that

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Martins, Luís Carlos. "Avaliação ultra-estrutural do endotélio corneal de ratos normais e de diabéticos aloxânicos /." Botucatu : [s.n.], 2002. http://hdl.handle.net/11449/88910.

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Orientador: Silvana Artioli Schellini<br>Resumo: O objetivo do estudo foi avaliar a influência do diabetes experimental sobre a ultra-estrutura do endotélio corneal de ratos. O estudo foi prospectivo, utilizando 20 ratos da raça Wistar, com 3 meses de idade, divididos em: grupo controle (GC), contendo 10 ratos, e grupo diabético (GD), contendo 10 ratos. A indução do diabetes foi feita com injeção de Aloxana endovenosa 42 mg/kg de peso (M0), após o que os animais foram observados por 15 dias para confirmar a presença de diabetes grave (M1). Um mês após M1 (M2) e 12 meses após M1 (M3) os animais

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8

Jones, Frances E. "The corneal endothelium in development, disease and surgery." Thesis, Cardiff University, 2013. http://orca.cf.ac.uk/49911/.

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Aims: The cornea is a tough, transparent tissue providing the primary refractive element of the eye. The stroma consists of specially arranged collagen required for corneal transparency. Correct stromal hydration is important in the maintenance of transparency, a feature controlled by the endothelial cells on the posterior surface of the cornea. The aims of this research were firstly to investigate the morphology of corneal endothelial cells and their expression of the sodium bicarbonate cotransporter during avian embryonic development and secondly, to clarify the effect of disease, surgery an

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Painter, Geoffrey Thomas. "Corneal Protection in Cataract Surgery." Thesis, The University of Sydney, 2019. https://hdl.handle.net/2123/21214.

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Protection of the corneal endothelium is one of the most important aspects of cataract surgery. This thesis describes two studies that examine new products and techniques to protect the cornea in cataract surgery. The first study examines the corneal protective effect of a new viscoelastic, DisCoVisc, compared to two established products in a randomised control trial of 180 patients. While no objective difference in corneal protection could be found between DisCoVisc and the other two viscoelastics, DisCoVisc compared more favourably in subjective surgical behaviour when compared to Healon alt

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Painter, Geoffrey Thomas. "Corneal Protection in Cataract Surgery." Thesis, The University of Sydney, 2019. https://hdl.handle.net/2123/21389.

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Protection of the corneal endothelium is one of the most important aspects of cataract surgery. This thesis describes two studies that examine new products and techniques to protect the cornea in cataract surgery. The first study examines the corneal protective effect of a new viscoelastic, DisCoVisc, compared to two established products in a randomised control trial of 180 patients. While no objective difference in corneal protection could be found between DisCoVisc and the other two viscoelastics, DisCoVisc compared more favourably in subjective surgical behaviour when compared to Healon alt

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11

Ojo, Victor Temidayo. "Postnatal Cell Shape development of the Corneal Endothelium in Mice." Digital Commons @ East Tennessee State University, 2017. https://dc.etsu.edu/etd/3301.

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Corneal endothelial cells have been shown to possess a uniform polygonal and complex multipolar shape at their apical and basolateral surface respectively. We established a morphological timetable to study how this complex shape arises postnatally. Corneas were collected from mice between postnatal day 8 to postnatal day 35 and labelled with antibodies specific for ZO-1 and NCAM at apical and basolateral region, respectively. Images were collected using wide-field fluorescence microscopy and morphometrically analyzed. Results showed that apical cell sizes increase linearly over the first 3 wee

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Bosch, Canals Begoña María. "A bioengineering approach for corneal endothelial regeneration." Doctoral thesis, Universitat Internacional de Catalunya, 2019. http://hdl.handle.net/10803/667398.

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Nowadays, there are approximately 10 million people worldwide with visual impairment due to corneal diseases. Currently, the main therapeutic solution is the transplant of a donor's cornea. The great majority of transplants is due to some failure in the inner layer of the cornea, which is called the corneal endothelium and this is mainly related with the inability of this layer to regenerate in vivo. However, transplants present several limitations such as the low number of healthy donors or immunological rejection by the patient. In order to overcome these problems, several researchers have

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13

Shivanna, Mahesh. "Breakdown of the barrier integrity in corneal endothelium by inflammatory stress." [Bloomington, Ind.] : Indiana University, 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3386723.

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Thesis (Ph.D.)--Indiana University, School of Optometry, 2009.<br>Title from PDF t.p. (viewed on Jul 22, 2010). Source: Dissertation Abstracts International, Volume: 70-12, Section: B, page: 7504. Adviser: Sangly P. Srinivas.

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Hünning, Paula Stieven. "Avaliação da morfologia pós-operatória das células do endotélio corneano de coelhos na região periférica perincisional comparativamente à região central." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2011. http://hdl.handle.net/10183/30846.

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A manutenção da morfologia normal do endotélio da córnea é um importante indicador da integridade funcional. A reparação endotelial frente a um trauma, em coelhos, ocorre por migração, hipertrofia e mitose celular. Objetivou-se comparar a morfologia das células do endotélio, da região periférica perincisional à região central, da córnea de coelhos (Oryctolagus cuniculus) em diferentes períodos pós-operatórios. Foram designados três grupos, com 5 animais cada, para avaliação pós-operatória, sendo G1 (7 dias); G2 (15 dias) e G3 (45 dias). Trinta bulbos dos olhos de coelhos, da raça Nova Zelândia

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15

Mgwebi, Thandiswa. "Morphological investigations into the development of the mammalian corneal endothelium using the mouse model." Doctoral thesis, University of Cape Town, 2004. http://hdl.handle.net/11427/3268.

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Includes bibliographical references (leaves 83-89).<br>The corneal endothelium (CE), a mesenchyme-derived tissue, is a monolayer of squamous cells on the inner corneal surface. In Foxc1-1• mice, the CE fails to form. The understanding of the cause of this defect has implications for the study of human eye disorders that are related to FOXC1 mutations. To understand the basis of CE defects in Foxc1-1- mice, an analysis of normal CE development was performed using scanning electron microscopy. Results showed that in normal mice the transformation from mesenchyme to endothelium was initiated at e

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Rudnick, David Jr. "Hyperadaptation - Another Missing Term in the Science of Form." Thesis, Virginia Tech, 1997. http://hdl.handle.net/10919/36886.

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In a 1982 paper, Gould and Vrba argue that a conflation of the two components of adaptation of a feature, historical development of the feature and present utility, has caused evolutionists to overlook a missing term in the science of form, which they call 'exaptation'. In the present project, I show that evolutionary biology still contains a confusion in the use of 'adaptation' due to an inappropriate perception of the interaction between the two components of adaptation. Because of the confusion, evolutionists have missed another term in the science of form. Evolutionary theory, specifica

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Pedemonte, Sarrias Eduard. "Tècnica de Muraine per a DMEK: anàlisi comparativa amb la tècnica estàndard." Doctoral thesis, Universitat Autònoma de Barcelona, 2017. http://hdl.handle.net/10803/405259.

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La queratoplàstia endotelial de la membrana de Descemet (DMEK) és la tècnica d'elecció actual per al tractament de l’edema corneal irreversible. Després que Melles la desenvolupés el 2006, Muraine va proposar el 2013 una tècnica alternativa per a la dissecció i implantació de l’empelt. Aquesta tècnica aportava dues novetats: la hidrodissecció amb trepanació parcial i curvatura corneal invertida del teixit donant i el plegament de l’empelt amb l'endoteli a la seva cara interna, que afavoria la protecció de l’endoteli i la tendència natural de l’empelt a desplegar-se a la cambra anterior del rec

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Kocaba, Viridiana. "Tissue engineering pour la reconstruction cornéenne." Thesis, Lyon, 2018. http://www.theses.fr/2018LYSE1078.

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En France, les dysfonctions endothéliales représentent environ la moitié des indications de greffes de cornée réalisées chaque année. Cependant, les problématiques liées à la pénurie de greffon, aux difficultés des techniques chirurgicales de greffes endothéliales ainsi qu’aux risques d’échec ou de rejet de greffe poussent les chercheurs à développer de nouvelles thérapies moins invasives et plus efficaces. La thérapie cellulaire cornéenne endothéliale est une des voies de recherche actuellement explorées dont le but est de s’affranchir des aléas de la greffe de cornée. La cornée humaine est u

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Mueller, Andreas. "Assessment of eye growth-related changes in the corneal endothelium of children and young teenagers." Thesis, Glasgow Caledonian University, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.341552.

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PINA, Fábio Luiz Silva. "Preservação de córneas de felinos domésticos (Felis catus - Linnaeus, 1758) em conservante utilizando a água de coco como meio nutritivo." Universidade Federal Rural de Pernambuco, 2007. http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5619.

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Submitted by (edna.saturno@ufrpe.br) on 2016-10-06T16:32:53Z No. of bitstreams: 1 Fabio Luiz Silva Pina.pdf: 1678514 bytes, checksum: add96ec2da4d0e8edf4922a29af1bb8c (MD5)<br>Made available in DSpace on 2016-10-06T16:32:53Z (GMT). No. of bitstreams: 1 Fabio Luiz Silva Pina.pdf: 1678514 bytes, checksum: add96ec2da4d0e8edf4922a29af1bb8c (MD5) Previous issue date: 2007-02-05<br>Corneal endotelium cells present the important function of osmotic pump and barrier, thus keeping it transparent. The loss of these cells, either by any reason, becomes the cornea cloudy, influencing negativety in the

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Oblak, Emil. "Assessment of welding ultraviolet radiation on the corneal endothelium using a newly developed computerized morphometric system." Thesis, Glasgow Caledonian University, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.289519.

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Zhang, Yan. "Studies on the calcium-regulated bicarbonate ion permeability in the apical membrane of bovine corneal endothelium." [Bloomington, Ind.] : Indiana University, 2004. http://wwwlib.umi.com/dissertations/fullcit/3162273.

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Thesis (Ph.D.)--Indiana University, School of Optometry, 2004.<br>Title from PDF t.p. (viewed Dec. 2, 2008). Source: Dissertation Abstracts International, Volume: 66-01, Section: B, page: 0050. Chair: Joseph A. Bonanno.

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Al, Abdulsalam Najla Khaled S. "Evaluation of silk fibroin as a scaffold for cultured corneal endothelial cell implants." Thesis, Queensland University of Technology, 2018. https://eprints.qut.edu.au/122231/1/Najla%20Khaled%20S_Al%20Abdulsalam_Thesis.pdf.

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Corneal transplants are a safe and effective treatment for corneal disease, but are hampered by the limited supply and quality of donor tissue. The goal of this project was therefore to evaluate the use of membranes prepared from silk protein as a scaffold on which to grow corneal tissue substitutes in the laboratory from corneal endothelial cells.

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Huang, Lan. "Endothelial Colony Forming Cells (ECFCs): Identification, Specification and Modulation in Cardiovascular Diseases." Thesis, Connect to resource online, 2009. http://hdl.handle.net/1805/2063.

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Thesis (Ph.D.)--Indiana University, 2009.<br>Title from screen (viewed on February 2, 2010). Department of Biochemistry and Molecular Biology, Indiana University-Purdue University Indianapolis (IUPUI). Advisor(s): Mervin C. Yoder, Jr., David A. Ingram, Jr., Lawrence A. Quilliam, Mark D. Pescovitz. Includes vitae. Includes bibliographical references (leaves 171-194).

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Corrêa, Luis Felipe Dutra. "Morfologia e morfometria das células do endotélio corneal de ovinos (Ovis aires) em diferentes faixas etárias obtidas pela microscopia especular." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2011. http://hdl.handle.net/10183/132917.

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Baseado na carência de documentação com respeito ao endotélio da córnea de ovinos, a pesquisa teve como objetivo documentar o endotélio corneal de ovinos em diferentes faixas etárias – jovem, adulto e velho – utilizando a microscopia especular. Logo, buscou-se investigar o efeito da idade sobre a morfologia e morfometria do endotélio da córnea de ovinos. O endotélio corneano foi avaliado com auxilio do microscópio especular de contato. No total, foram avaliados 18 ovinos (Ovis aires) neste estudo. Os animais foram distribuídos em três grupos de 6 animais cada um em função da idade. O grupo I c

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Hammadi, Shumoos T. H. "Novel medical imaging technologies for processing epithelium and endothelium layers in corneal confocal images. Developing automated segmentation and quantification algorithms for processing sub-basal epithelium nerves and endothelial cells for early diagnosis of diabetic neuropathy in corneal confocal microscope images." Thesis, University of Bradford, 2018. http://hdl.handle.net/10454/16924.

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Diabetic Peripheral Neuropathy (DPN) is one of the most common types of diabetes that can affect the cornea. An accurate analysis of the corneal epithelium nerve structures and the corneal endothelial cell can assist early diagnosis of this disease and other corneal diseases, which can lead to visual impairment and then to blindness. In this thesis, fully-automated segmentation and quantification algorithms for processing and analysing sub-basal epithelium nerves and endothelial cells are proposed for early diagnosis of diabetic neuropathy in Corneal Confocal Microscopy (CCM) images. Firstly,

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Franzen, Angela Aguiar [UNESP]. "Morfologia e morfometria do endotélio corneal de gatos (Felis catus - Linnaeus, 1758) de diferentes idades à microscopia especular." Universidade Estadual Paulista (UNESP), 2008. http://hdl.handle.net/11449/89021.

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Made available in DSpace on 2014-06-11T19:23:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-08-04Bitstream added on 2014-06-13T18:51:10Z : No. of bitstreams: 1 franzen_aa_me_jabo.pdf: 432317 bytes, checksum: 6e76f84b35fefc0d8afc5bb52873a43f (MD5)<br>Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)<br>O endotélio corneal é essencial para a manutenção da transparência da córnea, é necessário um número mínimo de células endoteliais para a manutenção desta transparência. As alterações morfológicas e morfométricas do endotélio da córnea de gatos decorrentes do avanç

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Franzen, Angela Aguiar. "Morfologia e morfometria do endotélio corneal de gatos (Felis catus - Linnaeus, 1758) de diferentes idades à microscopia especular /." Jaboticabal : [s.n.], 2008. http://hdl.handle.net/11449/89021.

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Resumo: O endotélio corneal é essencial para a manutenção da transparência da córnea, é necessário um número mínimo de células endoteliais para a manutenção desta transparência. As alterações morfológicas e morfométricas do endotélio da córnea de gatos decorrentes do avanço da idade não estão ainda compreendidas e elucidadas. Objetivou-se estudar os parâmetros morfológicos e morfométricos do endotélio da córnea de gatos de diferentes idades, utilizando-se a microscopia especular. Avaliaram-se a densidade celular, a área celular média, o pleomorfismo e o polimegatismo. Empregaram-se 18 animais

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Albuquerque, Luciane de. "Avaliação das repercussões do transplante lamelar posterior sobre o endotélio corneano de suínos utilizando a microscopia eletrônica de varredura. Estudo in vitro." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2014. http://hdl.handle.net/10183/103436.

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O transplante endotelial tem sido cada vez mais utilizado como uma alternativa ao transplante penetrante no tratamento das desordens do endotélio da córnea. No entanto, ao consultar a literatura não foram encontradas referências identificando em qual momento da cirurgia de transplante lamelar posterior ocorre maior dano às células endoteliais do botão doador. Objetivou-se avaliar e comparar as repercussões de duas etapas do transplante lamelar posterior sobre o endotélio da córnea de suínos utilizando a microscopia eletrônica de varredura. Utilizaram-se 30 bulbos oculares de 20 suínos, mestiço

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Faganello, Cláudia Skilhan. "Morfologia celular endotelial de diferentes regiões da córnea de equinos (Equus caballus) com a coloração vermelho de alizarina." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2014. http://hdl.handle.net/10183/108179.

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Objetivo: O endotélio é uma monocamada de células achatadas, poligonais e interligadas que recobrem a superfície posterior da córnea, sendo fundamental na manutenção da transparência desta estrutura. Objetivou-se avaliar a morfologia de diferentes regiões da córnea de equinos após coloração com vermelho de alizarina utilizando a microscopia óptica. Procedimentos: Foram estudados 16 bulbos oculares de oito equinos, machos ou fêmeas, de diferentes faixas etárias. O endotélio da córnea foi corado com o corante vital vermelho de alizarina (Alizarin Red S, Sigma Aldrich), dissolvido previamente em

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Valtink, Monika, Rita Gruschwitz, Richard H. W. Funk, and Katrin Engelmann. "Two Clonal Cell Lines of Immortalized Human Corneal Endothelial Cells Show either Differentiated or Precursor Cell Characteristics." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-136199.

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Access to primary human corneal endothelial cells (HCEC) is limited and donor-derived differences between cultures exacerbate the issue of data reproducibility, whereas cell lines can provide sufficient numbers of homogenous cells for multiple experiments. An immortalized HCEC population was adapted to serum-free culture medium and repeated cloning was performed. Clonally grown cells were propagated under serum-free conditions and growth curves were recorded. Cells were characterized immunocytochemically for junctional proteins, collagens, Na,K-ATPase and HCEC-specific 9.3.E-antigen. Ultrastru

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Valtink, Monika, Rita Gruschwitz, Richard H. W. Funk, and Katrin Engelmann. "Two Clonal Cell Lines of Immortalized Human Corneal Endothelial Cells Show either Differentiated or Precursor Cell Characteristics." Karger, 2008. https://tud.qucosa.de/id/qucosa%3A27701.

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Access to primary human corneal endothelial cells (HCEC) is limited and donor-derived differences between cultures exacerbate the issue of data reproducibility, whereas cell lines can provide sufficient numbers of homogenous cells for multiple experiments. An immortalized HCEC population was adapted to serum-free culture medium and repeated cloning was performed. Clonally grown cells were propagated under serum-free conditions and growth curves were recorded. Cells were characterized immunocytochemically for junctional proteins, collagens, Na,K-ATPase and HCEC-specific 9.3.E-antigen. Ultrastru

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Almeida, Ana Carolina da Veiga Rodarte de. "Avaliação clínica e pela microscopia eletrônica de varredura do adesivo de fibrina, comparativamente ao fio de sutura na oclusão da incisão de córnea: estudo experimental em coelhos." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2009. http://hdl.handle.net/10183/16293.

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As técnicas de remoção de catarata evoluíram nas últimas décadas. Na tentativa de oclusão da córnea após incisão para remoção da catarata, diversas técnicas têm sido propostas. Objetivou-se avaliar experimentalmente a viabilidade do emprego do adesivo de fibrina na oclusão da incisão de córnea em coelhos. Além disso, comparar os efeitos do adesivo de fibrina e do fio de sutura na oclusão da incisão de córnea em coelhos, utilizando-se os aspectos clínicos, a microscopia eletrônica de varredura e a morfometria. Dezesseis coelhos (Oryctolagus cuniculus) da raça Nova Zelândia foram submetidos à in

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Marcon, Alexandre Seminoti. "Influência da espessura corneana na acuidade visual corrigida após transplante de córnea endotelial lamelar profundo (TCELP)." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/5/5149/tde-16102014-085907/.

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Objetivo: Analisar a influência da espessura corneana central na acuidade visual (AV) corrigida após transplante de córnea endotelial lamelar profundo (TCELP). Métodos: Foram estudados de forma prospectiva 155 olhos de 127 pacientes portadores de ceratopatia bolhosa ou distrofia endotelial de Fuchs no sexto mês de pós-operatório do TCELP, entre março de 2000 e março de 2005. Foram excluídos pacientes com outras alterações oculares que justificassem baixa AV. Todos os pacientes foram submetidos à avaliação oftálmica, quando foram determinadas AV corrigida, por meio de exame refratométrico, e es

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Andrade, Maria Cristina Caldart de. "Avaliação do endotélio corneano de equinos após exposição ao corante azul brilhante 0,05% - estudo in vitro." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2016. http://hdl.handle.net/10183/143947.

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Os corantes vitais têm sido utilizados em diversas áreas da oftalmologia para melhorar a visibilização de diferentes tecidos. Com o objetivo de avaliar os efeitos imediatos do corante Azul brilhante a 0,05% no endotélio da córnea de equinos, foram utilizadas 38 córneas de 19 cavalos, machos ou fêmeas, de diferentes idades e provenientes de abatedouro comercial licenciado. As córneas foram aleatoriamente divididas em dois grupos. Grupo1: o endotélio da córnea foi exposto a solução salina balanceada por 60 segundos. Grupo 2: o endotélio da córnea foi exposto ao corante Azul brilhante 0,05% (Opht

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Silva, Vanessa Ruiz Moura da. "Avaliação do endotélio corneano de equinos após exposição à indocianina verde 0,5% : estudo in vitro." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2014. http://hdl.handle.net/10183/108171.

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A capsulotomia curvilínea contínua (CCC) é uma das etapas mais importantes da técnica de facoemulsificação. Em cataratas brancas e com reflexo de fundo de olho deficiente ou ausente, a identificação da cápsula anterior do cristalino é dificultada, e é necessária a utilização de substâncias, como os corantes vitais, para permitir a sua diferenciação. Contudo, antes da utilização de substâncias intraoculares, é necessário determinar se elas são seguras para as estruturas do globo ocular, principalmente para as células do endotélio corneano, que podem sofrer lesões irreversíveis. A indocianina ve

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Freitas, Luciana Vicente Rosa Pacicco de. "Avaliação do endotélio da córnea de galinhas (Gallus gallus domesticus) em diferentes faixas etárias utilizando a microscopia especular." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2012. http://hdl.handle.net/10183/55971.

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O endotélio corneano é uma monocamada de células poligonais localizadas na face posterior da córnea e é essencial para a manutenção da transparência corneana. Não foram encontradas referências na literatura a respeito dos parâmetros morfológicos e morfométricos do endotélio da córnea de galinhas, apesar destes animais serem amplamente utilizados como modelo experimental em estudos oftálmicos, devido à similitude com a córnea de humanos. O objetivo deste estudo foi o de avaliar os parâmetros morfométricos e o pleomorfismo da região central do endotélio da córnea de galinhas (Gallus gallus domes

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Tessarioli, Mariana. "Avaliação do endotélio corneano suíno por microscopia eletrônica de varredura após aplicação de azul brilhante a 0,05% na câmara anterior – Estudo in vitro." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2013. http://hdl.handle.net/10183/76541.

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Diversos corantes vitais vêm sendo estudados e utilizados para a facilitação da capsulotomia curvilínea contínua (CCC) nas cirurgias de catarata no homem e nos animais. Além de corar adequadamente a cápsula anterior da lente e favorecer um melhor desempenho do cirurgião durante a realização da CCC, os corantes vitais devem ser seguros quanto aos seus efeitos sobre as estruturas oculares, em especial ao endotélio corneano, quando empregados com esta finalidade. O azul brilhante é um corante vital já empregado em cirurgias oculares do segmento posterior para coloração da retina e atualmente estu

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Bollu, Lakshmi Reddy. "The Effect of Endothelin-1 on the expression of CDK Inhibitors p21 & p27 in Bovine Corneal Endothelial Cells." TopSCHOLAR®, 2009. http://digitalcommons.wku.edu/theses/112.

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Mammalian corneal endothelial cells are considered to be non-proliferative due to the arrest of cells at the G1 phase of the cell cycle. The purpose of this study was to determine whether the down regulation of cyclin dependant kinase inhibitors (p21cip1 and p27kip1) levels by Endothelin-1 (ET-1), would overcome the G1 phase arrest and promote cell cycle progression and proliferation in cultured BCECs (Bovine corneal endothelial cells). BCECs were isolated from bovine corneas and cultured in DMEM supplemented with 10% serum. 5-Bromo 2-deoxy Uridine (BrdU) incorporation was determined in serum

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Bethi, Akhila. "Endothelin-1 Induced Phosphorylation of ERK1/2 in Bovine Corneal Endothelial Cells." TopSCHOLAR®, 2012. http://digitalcommons.wku.edu/theses/1191.

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The purpose of this study was to determine whether Endothelin-1 (ET-1) induced cellular responses in bovine corneal endothelial cells (BCECs) involves MAPK pathway by phosphorylating ERK1/2 protein kinase and to find out the phosphorylation patterns of ERK1/2 in confluent and sub-confluent cells. BCECs were isolated from bovine corneas and cultured in medium supplemented with 10% serum. Confluent (contact inhibited) and sub-confluent (actively growing cells) serum starved cells grown in T-75 flasks were treated with 10nM Endothelin-1. The control cells were left untreated. Total cellular prote

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Silva, Géssica Maria Ribeiro da. "Morfologia e morfometria das células do endotélio da córnea de equinos utilizando a microscopia eletrônica de varredura." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2018. http://hdl.handle.net/10183/181327.

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O conhecimento da morfologia endotelial nas diferentes regiões da córnea é de suma importância para avaliação de endotélios corneanos saudáveis e doentes e de suas respostas ao uso de medicações. O objetivo do presente estudo foi descrever a morfologia do endotélio nas regiões central e periférica superior da córnea saudável de equinos, medir a área média das células pentagonais, hexagonais e heptagonais presentes nas regiões avaliadas, calcular o polimegatismo de cada tipo celular e correlacionar estes parâmetros entre os diferentes formatos celulares. Foram estudados dez equinos, machos ou f

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Holzchuh, Ricardo. "Estudo da reprodutibilidade do exame de microscopia especular de córnea em amostras com diferentes números de células." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/5/5149/tde-01122011-111704/.

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INTRODUÇÃO: O endotélio corneal exerce papel primordial para a fisiologia da córnea. Seus dados morfológicos gerados pelo microscópio especular (MEC) como densidade endotelial (DE), área celular média (ACM), coeficiente de variação (CV) e porcentagem de células hexagonais (HEX) são importantes para avaliar sua vitalidade. Para interpretar estes dados de forma padronizada e reprodutível, foi utilizado um programa estatístico de análise amostral, Cells Analyzer PAT. REQ.(CA). OBJETIVO: Demonstrar valores de referência para DE, ACM, CV e HEX. Demonstrar o percentual de células endoteliais marcada

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Chan, Giulia. "Regulation of viability in corneal endothelial cells." Thesis, University College London (University of London), 2005. http://discovery.ucl.ac.uk/1444592/.

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The major cause of corneal opacity and resultant visual loss is a critical decrease in corneal endothelial cell density. Due to the fact that corneal endothelial cells do not generally proliferate, cell densities gradually decrease in the corneal endothelium with age. Before we can begin to aid patients with decreasing endothelial cell densities, by inhibiting cell death or stimulating cell proliferation, it is necessary to understand the basic cell signalling that underlies these processes. As human tissue is difficult to obtain, a representative animal model of corneal endothelium was devise

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Rolev, Kostadin Dimov. "Evaluation of corneal endothelial cell therapy using an in vitro human corneal model." Thesis, Anglia Ruskin University, 2017. http://arro.anglia.ac.uk/702583/.

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Aim: To establish an in vitro human corneal decompensation model and to use it for the evaluation of a cell-therapy approach for treating corneal endothelial (CE) disorders and to test the expression profile of positive regulatory domain proteins (PRDMs) as potential markers for corneal endothelial cells (CECs). Materials and Methods: Human cadaveric corneas were obtained from Bristol and Manchester Eye Banks, UK. A CE decompensation model was established by removal of the Descemet’s membrane (DM)/Endothelium complex from donor corneas and placing them in air-interface organ culture. The corne

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Wenzel, Daniel Alexander [Verfasser]. "Auswirkungen von Perfluorbutylpentan (F4H5) auf corneale Endothelzellen im porcinen Hornhautmodell : Effects of perfluorobutylpentan (F4H5) on corneal endothelial cells / Daniel Alexander Wenzel." Hamburg : Staats- und Universitätsbibliothek Hamburg Carl von Ossietzky, 2019. http://d-nb.info/1223621057/34.

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Elzawia, Omar Rajab. "Corneal graft outcome, endothelial monolayer survival following corneal grafting : a prospective and retrospective study." Thesis, University of Bristol, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.322567.

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Dannowski, Haike. "Gentransfer in korneale Endothelzellen." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2004. http://dx.doi.org/10.18452/15137.

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Bei der Hornhauttransplantation (Keratoplastik) handelt es sich um die häufigste Transplantation humanen Gewebes. Das Hornhautendothel, eine empfindliche Zellschicht auf der Innenseite der Kornea, verfügt nicht über die Fähigkeit, Zellverluste durch Proliferation auszugleichen. Zwei grundsätzliche Probleme ergeben sich aus dieser Eigenschaft des kornealen Endothels für die Keratoplastik: ein Endothelzellverlust tritt zum einen während der Hornhautkonservierung vor Keratoplastik auf und führt zu einem Ausschluss vieler Transplantate, zum anderen ist er oftmals im Zuge von immunvermi

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Yu, Wing-yan, and 余泳欣. "Study of stem/progenitor cells located in the posterior limbus of the eye." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/197119.

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Some stem-like cells that have received limited attention but may be of considerable clinical value reside in the transition zone between the corneal endothelium (CE) and trabecular meshwork (TM) at the posterior limbus of the eyes. A growing body of evidence has revealed that these cells may be able to rejuvenate the CE, TM or both. However, these stem-like cells have not been clearly defined and we have thus named them PET cells (Progenitor cells of the Endothelium and Trabeculum). Both the CE and TM cells are unique cell types in the eye that do not normally replace themselves once they

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Laverne-Acquart, Sophie. "Banque de cornée : 10 ans d’innovations en contrôle qualité du greffon cornéen et projets d’avenir." Thesis, Saint-Etienne, 2013. http://www.theses.fr/2013STET012T/document.

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Le contrôle qualité réalisé sur les cornées en organoculture dans les banques de cornées est primordial pour l'évaluation de la qualité des greffons. Dans une première partie, nous avons montré, sur 505 cornées en routine, que les paramètres mesurés avec notre analyseur d'images Sambacornea (CV de taille et hexagonalité cellulaire endothéliale) étaient tout à fait cohérents avec ceux mesurés en microscopie spéculaire in vivo chez les patients. Notre dispositif fournit une méthode fiable, validée pour la mesure de la densité cellulaire endothéliale DCE et de la morphométrie cellulaire. Dans une

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Valtink, Monika, Mirko Nitschke, Thomas Götze, Katrin Engelmann, and Carsten Werner. "Kultivierung transplantierbarer Zellverbände aus cornealem Endothel." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2008. http://nbn-resolving.de/urn:nbn:de:bsz:14-ds-1223722766870-16827.

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Die In-vitro-Kultivierung von cornealem Endothel, einer funktionalen, beim Menschen nicht regenerierbaren Schicht in der Hornhaut des Auges, eröffnet weitreichende Möglichkeiten zum Zell- und Gewebeersatz. Dieser Artikel beschreibt aktuelle und künftige Optionen für zellbasierte Therapieansätze sowie die Bedeutung unbegrenzt proliferationsfähiger (immortalisierter) Zellpopulationen als Modellsystem für die Entwicklung neuartiger Methoden. In diesem Zusammenhang werden schaltbare Zellkulturträger als Möglichkeit zur schonenden Gewinnung transplantierbarer „cell sheets“ vorgestellt. Darüber hina

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