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Artykuły w czasopismach na temat „Corneal endothelium”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 25 lipca 2025

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1

Chirila, Traian V., Peter W. Madden, and Lawrie W. Hirst. "Replacement of the Corneal Endothelium and the Conceptual Framework for an Artificial Substitute." Journal of Biomimetics, Biomaterials and Tissue Engineering 5 (February 2010): 13–29. http://dx.doi.org/10.4028/www.scientific.net/jbbte.5.13.

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Dysfunction of the corneal endothelium due to cell loss caused by aging, disease or trauma can lead to severe visual impairment and blindness. Traditionally, dysfunctional endothelia are managed surgically, by removing the entire central cornea and transplanting either donor corneal tissue (penetrating keratoplasty), or just endothelia isolated from donor corneas. As in many cases it is only the corneal endothelium requiring replacement, many attempts were made over the last decades to develop an endothelial substitute, thereby precluding the need for the use of full donor corneas. This articl

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Ong, Keith. "SLT may compromise the corneal endothelium." Asian Journal of Ophthalmology 13, no. 3 (2014): 80–85. http://dx.doi.org/10.35119/asjoo.v13i3.129.

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Purpose: Whitish spots are sometimes noted in the corneal endothelium after Selective Laser Trabeculoplasty (SLT). One wonders whether this could be laser burns to the corneal endothelium. To evaluate the corneal endothelium after SLT, corneal specular microscopy was performed before and after SLT.Method: 20 patients with open angle glaucoma, who had SLT in February-March 2012, had their corneal endothelium examined with specular microscopy before and after SLT.Results: 4 of the 20 patients showed numerous dark patches/spots on specular microscopy photographs of corneal endothelium after SLT.

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Ostrovski, D. S., S. A. Borzenok, B. E. Malyugin, O. P. Antonova, M. Kh Khubetsova та T. Z. Kerimov. "Проблема получения клеточной культуры эндотелиальных клеток роговицы для регенеративных целей". Russian Journal of Transplantology and Artificial Organs 26, № 2 (2024): 135–44. http://dx.doi.org/10.15825/1995-1191-2024-2-135-144.

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Human posterior corneal epithelium (corneal endothelium) has limited proliferative activity both in vivo and in vitro. Disease or dysfunction in these cells leads to impaired corneal transparency of varying degrees of severity, up to blindness. Currently, the only effective standard treatment for corneal endothelial dysfunction is transplantation of donor cornea that contains a pool of healthy and functionally active cells. However, there is a global shortage of donor corneas, which has led to an unmet clinical need and the fact that only 1 patient out of 10 in need receives surgical treatment

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Guimarães, Celeste B., Luciane Albuquerque, Marcela Torikachvili, et al. "Effects of atracurium besylate on corneal endothelium of chickens: in vitro study." Pesquisa Veterinária Brasileira 39, no. 1 (2019): 70–74. http://dx.doi.org/10.1590/1678-5150-pvb-5595.

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ABSTRACT: The aim of this study was to investigate the acute effects of atracurium besylate on cellular damage in corneal endothelium of chickens. Twenty healthy chicken eyes were assigned to the following groups: Group 1 (G1), experimental group (n=10); and Group 2 (G2), control (n=10). Excised corneoscleral buttons were immediately placed on glass microscopy slides with endothelial region faced up. Corneal endothelium of eyes in G1 were covered with AB (0.2mL, 10mg/mL) for 3 min and then rinsed with balanced salt solution (BSS), while the corneal endothelium of eyes in G2 were covered with B

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Wang, Xuemei, Yanlin Zhong, Minghui Liang, Zhirong Lin, Huping Wu, and Cheng Li. "Crosslinking-Induced Corneal Endothelium Dysfunction and Its Protection by Topical Ripasudil Treatment." Disease Markers 2022 (January 13, 2022): 1–12. http://dx.doi.org/10.1155/2022/5179247.

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Purpose. To investigate the changes of corneal endothelium under different crosslinking conditions and the protective effect of ripasudil. Methods. Corneal crosslinking groups were infiltrated with riboflavin and subsequently irradiated with 0.54 J/cm2 or 1.08 J/cm2 UVA, while noncrosslinking groups included neither UVA nor riboflavin treatment, only 1.08 J/cm2 UVA and only riboflavin treatment. Corneal opacity, variations in corneal endothelial cells, and corneal thickness of all groups were observed by slit lamp, in vivo confocal microscopy, and optical coherence tomography. Immunofluorescen

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Andrade, M. C. C., T. M. Moreno, M. S. Muccillo, J. A. T. Pigatto, and E. V. Camilo. "Evaluation of equine corneal endothelium after exposure to 0.05% brilliant blue - an in vitro study." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 71, no. 4 (2019): 1158–64. http://dx.doi.org/10.1590/1678-4162-9969.

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ABSTRACT The aim of this study was to evaluate the immediate effects of 0.05% brilliant blue on corneal endothelium of horses. Thirty-eight corneas of 19 horses, male or female, of different ages were studied. Corneas were randomly divided into two groups. Group 1: Corneal endothelium was covered with 0.3mL of brilliant blue 0.05% for 60 seconds followed by rinsing with a balanced salt solution. Group 2: Corneal endothelium was covered with BSS for 60 seconds. The corneas were excised with an 8mm trephine and prepared to analyze posterior endothelial surface using a light microscope (24 cornea

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Hussain, Noor Ahmed, Francisco C. Figueiredo, and Che J. Connon. "Use of biomaterials in corneal endothelial repair." Therapeutic Advances in Ophthalmology 13 (January 2021): 251584142110582. http://dx.doi.org/10.1177/25158414211058249.

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Human corneal endothelium (HCE) is a single layer of hexagonal cells that lines the posterior surface of the cornea. It forms the barrier that separates the aqueous humor from the rest of the corneal layers (stroma and epithelium layer). This layer plays a fundamental role in maintaining the hydration and transparency of the cornea, which in turn ensures a clear vision. In vivo, human corneal endothelial cells (HCECs) are generally believed to be nonproliferating. In many cases, due to their nonproliferative nature, any damage to these cells can lead to further issues with Descemet’s membrane

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Chowdhary, Aakash, Anshu Sharma, Marisha Bishnoi, and Priyanka Dubey. "Scratched cornea: Case report on incidental bilateral posterior polymorphous corneal dystrophy." Indian Journal of Ophthalmology - Case Reports 3, no. 4 (2023): 988–90. http://dx.doi.org/10.4103/ijo.ijo_564_23.

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Posterior polymorphous corneal dystrophy (PPCD) is a dominantly inherited corneal endothelial disorder, typically considered bilateral and asymptomatic. We report a case of bilateral PPCD that presented as an incidental finding on routine ophthalmic examination. Related visual prognosis is discussed. A 27-year-old male presented for a routine ophthalmic examination. Visual acuity was recorded for both eyes. A detailed slit-lamp examination and anterior-segment optical coherence tomography (AS-OCT) was done. Best-corrected visual acuity was recorded as 6/6 for both eyes. Slit-lamp examination r

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9

Luo, Xiaoying, Xin He, Hui Zhao, et al. "Research Progress of Polymer Biomaterials as Scaffolds for Corneal Endothelium Tissue Engineering." Nanomaterials 13, no. 13 (2023): 1976. http://dx.doi.org/10.3390/nano13131976.

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Nowadays, treating corneal diseases arising from injury to the corneal endothelium necessitates donor tissue, but these corneas are extremely scarce. As a result, researchers are dedicating significant efforts to exploring alternative approaches that do not rely on donor tissues. Among these, creating a tissue-engineered scaffold on which corneal endothelial cells can be transplanted holds particular fascination. Numerous functional materials, encompassing natural, semi-synthetic, and synthetic polymers, have already been studied in this regard. In this review, we present a comprehensive overv

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Terzariol, Mariana, Paula S. Hünning, Gustavo Brambatti, Luciane de Albuquerque, Carolina Neumann, and João A. T. Pigatto. "Effects of intracameral brilliant blue on the corneal endothelium of swine: in vitro study." Pesquisa Veterinária Brasileira 36, no. 8 (2016): 775–80. http://dx.doi.org/10.1590/s0100-736x2016000800016.

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Abstract: The aim was to investigate the ultrastructural changes in the corneal endothelium of pigs induced by intracameral 0.05% brilliant blue. Twenty swine corneas were separated into two groups, the right eye bulbs (control group) and the left eye bulbs (experimental group) of the same animal. All the eye bulbs were evaluated with specular microscopy. The cornea of the right eye bulbs was excised and in the left eye bulbs 0.2ml of 0.05% brilliant blue vital dye (OPTH-blue±) was injected into the anterior chamber, where it remained for one minute. Then the anterior chamber was cleaned with

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Azevedo, Mariane, Nat ndez, Lu Cargnin, et al. "Specular microscopy of the corneal endothelial cells of bovines: An ex vivo study." Open Veterinary Journal 13, no. 12 (2023): 1554. http://dx.doi.org/10.5455/ovj.2023.v13.i12.5.

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Background: The endothelium is the most posterior layer of the cornea and essential for maintaining corneal transparency. Due to variations in corneal endothelial parameters among different species, knowledge of the normal parameters for each species is crucial. Aim: To evaluate the corneal endothelium of bovines using contact specular microscopy. Methods: Twenty eyeballs from 10 male Brangus (Bos taurus) aged 24 months were evaluated. Contact specular microscopy was performed on the central corneal area. The analysed parameters were endothelial cell density (ECD) and endothelial cell morpholo

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12

Baturina, G. S., I. G. Palchikova, A. A. Konev, et al. "STUDY OF THE EFFECT OF HYPOTHERMIC CONSERVATION ON THE INTRACELLULAR SODIUM CONCENTRATION IN THE ENDOTHELIUM OF CORNEAL TRANSPLANTS." Vavilov Journal of Genetics and Breeding 22, no. 4 (2018): 433–37. http://dx.doi.org/10.18699/vj18.379.

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Endothelial keratoplasty has become the treatment of choice for corneal endothelial dysfunction. Advancements in the surgical treatment of corneal endothelial diseases depend on progress in graft conservation and its related advantages in assessing the suitability of grafts for transplantation. Transport of water and ions by cornea endothelium is important for the optic properties of cornea. In this work, we study the intracellular sodium concentration in cornea endothelial cells in samples of pig cornea that underwent hypothermic conservation for 1 and 10 days and endothelial cells of human c

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Feizi, Sepehr. "Corneal endothelial cell dysfunction: etiologies and management." Therapeutic Advances in Ophthalmology 10 (January 2018): 251584141881580. http://dx.doi.org/10.1177/2515841418815802.

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A transparent cornea is essential for the formation of a clear image on the retina. The human cornea is arranged into well-organized layers, and each layer plays a significant role in maintaining the transparency and viability of the tissue. The endothelium has both barrier and pump functions, which are important for the maintenance of corneal clarity. Many etiologies, including Fuchs’ endothelial corneal dystrophy, surgical trauma, and congenital hereditary endothelial dystrophy, lead to endothelial cell dysfunction. The main treatment for corneal decompensation is replacement of the abnormal

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Zhao, Can, Wenjing Li, Haoyun Duan, et al. "NAD+ precursors protect corneal endothelial cells from UVB-induced apoptosis." American Journal of Physiology-Cell Physiology 318, no. 4 (2020): C796—C805. http://dx.doi.org/10.1152/ajpcell.00445.2019.

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Excessive exposure of the eye to ultraviolet B light (UVB) leads to corneal edema and opacification because of the apoptosis of the corneal endothelium. Our previous study found that nicotinamide (NIC), the precursor of nicotinamide adenine dinucleotide (NAD), could inhibit the endothelial-mesenchymal transition and accelerate healing the wound to the corneal endothelium in the rabbit. Here we hypothesize that NIC may possess the capacity to protect the cornea from UVB-induced endothelial apoptosis. Therefore, a mouse model and a cultured cell model were used to examine the effect of NAD+ prec

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Bryant, M. R., and P. J. McDonnell. "A Triphasic Analysis of Corneal Swelling and Hydration Control." Journal of Biomechanical Engineering 120, no. 3 (1998): 370–81. http://dx.doi.org/10.1115/1.2798004.

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Physiological studies strongly support the view that hydration control in the cornea is dependent on active ion transport at the corneal endothelium. However, the mechanism by which endothelial ion transport regulates corneal thickness has not been elaborated in detail. In this study, the corneal stroma is modeled as a triphasic material under steady-state conditions. An ion flux boundary condition is developed to represent active transport at the endothelium. The equations are solved in cylindrical coordinates for confined compression and in spherical coordinates to represent an intact cornea

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Рыков, С. А., М. Г. Лысенко, И. В. Шаргородская, et al. "Endothelial Cells Survival in Human Corneal Grafts When Using the Modified Method Treatment of Keratoconus." Офтальмология. Восточная Европа, no. 3 (November 27, 2020): 311–27. http://dx.doi.org/10.34883/pi.2020.10.3.019.

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Проведена лазерная сканирующая конфокальная микроскопия донорских трупных роговиц, предназначенных для кератопластики. С помощью программного обеспечения ImageJ проанализирована рефлективность эндотелия и введено понятие коэффициента рефлективности эндотелиальных клеток роговицы (КРЭКР). В ходе статистической обработки определены морфометрические свойства эндотелиоцитов при конфокальной микроскопии, которые существенно влияют на потерю эндотелиальных клеток на трансплантате и, возможно, являются признаками апоптоза эндотелиоцитов. Разработана методика вископротекции эндотелия кадаверной рогови

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Sun, Xing Cai, Joseph A. Bonanno, Sergey Jelamskii, and Qiang Xie. "Expression and localization of Na+-HCO3 − cotransporter in bovine corneal endothelium." American Journal of Physiology-Cell Physiology 279, no. 5 (2000): C1648—C1655. http://dx.doi.org/10.1152/ajpcell.2000.279.5.c1648.

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Functional studies support the presence of the Na+-HCO3 −cotransporter (NBC) in corneal endothelium and possibly corneal epithelium; however, molecular identification and membrane localization have not been reported. To test whether NBC is expressed in bovine cornea, Western blotting was performed, which showed a single band at ∼130 kDa for freshly isolated and cultured endothelial cells, but no band for epithelium. Two isoforms of NBC have recently been cloned in kidney (kNBC) and pancreas (pNBC). RT-PCR was run using cultured and fresh bovine corneal endothelial and fresh corneal epithelial

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Lovatt, Matthew, Khadijah Adnan, Gary Peh, and Jodhbir Mehta. "Regulation of Oxidative Stress in Corneal Endothelial Cells by Prdx6." Antioxidants 7, no. 12 (2018): 180. http://dx.doi.org/10.3390/antiox7120180.

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The inner layer of the cornea, the corneal endothelium, is post-mitotic and unable to regenerate if damaged. The corneal endothelium is one of the most transplanted tissues in the body. Fuchs’ endothelial corneal dystrophy (FECD) is the leading indication for corneal endothelial transplantation. FECD is thought to be an age-dependent disorder, with a major component related to oxidative stress. Prdx6 is an antioxidant with particular affinity for repairing peroxidised cell membranes. To address the role of Prdx6 in corneal endothelial cells, we used a combination of biochemical and functional

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Vallabh, Neeru A., Stephnie Kennedy, Riccardo Vinciguerra, et al. "Corneal Endothelial Cell Loss in Glaucoma and Glaucoma Surgery and the Utility of Management with Descemet Membrane Endothelial Keratoplasty (DMEK)." Journal of Ophthalmology 2022 (January 30, 2022): 1–17. http://dx.doi.org/10.1155/2022/1315299.

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The corneal endothelium has a crucial role in maintaining a clear and healthy cornea. Corneal endothelial cell loss occurs naturally with age; however, a diagnosis of glaucoma and surgical intervention for glaucoma can exacerbate a decline in cell number and impairment in morphology. In glaucoma, the mechanisms for this are not well understood, and this accelerated cell loss can result in corneal decompensation. Given the high prevalence of glaucoma worldwide, this review aims to explore the abnormalities observed in the corneal endothelium in differing glaucoma phenotypes and glaucoma therapi

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Mishra, Neeta, Neelam Prasad, Rekha Chotalia, Uday Bajare, and Veshal V. Madan. "Millennial Generation’s Choice of Contact Lens as the Preference Over Spectacles: A Study of Corneal Endothelial Cell Density." Medical Journal of Dr. D.Y. Patil Vidyapeeth 17, no. 5 (2024): 1004–6. http://dx.doi.org/10.4103/mjdrdypu.mjdrdypu_750_23.

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ABSTRACT Introduction: The cornea of the eye is the most vulnerable organ to external insult. Contact lens is no exception. Changes in corneal epithelium, stroma, and loss of hexagonal shape in the endothelium have been taught in contact lens classes. We wanted to understand the cell density of endothelium in prolonged contact lens wear. Methods: It was a prospective, observational, cross-sectional study. The volunteers were divided into two groups: group 1 wore their contact lenses for over 5 years and group 2 never wore contact lenses. Specular microscopy was performed using Topcon SP-3000P.

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Malyugin, B. E., S. A. Borzenok, O. P. Antonova, D. S. Ostrovskii, and Z. R. Ebzeeva. "Transplantation of endothelial cell suspension in an ex vivoexperiment." Fyodorov journal of ophthalmic surgery, no. 4 (December 28, 2023): 86–92. http://dx.doi.org/10.25276/0235-4160-2023-4-86-92.

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Relevance. Corneal transplantation with entire cornea or anormal layers replacement remains the only treatment option for patients with corneal endothelium pathology. Selective endothelial replacement was introduced into practice two decades ago, namely posterior lamellar keratoplasty, which has advantages over penetrating keratoplasty. Improvements of this method led to technique creation where isolated Descemet's membrane with endothelium monolayer without stromal layer is transplanted. This technology is associated with risks of intra- and postoperative complications, and is difficult to pe

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Wang, De-An, Haiming Du, Jonathan H. Jaggar, David N. Brindley, Gabor J. Tigyi, and Mitchell A. Watsky. "Injury-elicited differential transcriptional regulation of phospholipid growth factor receptors in the cornea." American Journal of Physiology-Cell Physiology 283, no. 6 (2002): C1646—C1654. http://dx.doi.org/10.1152/ajpcell.00323.2002.

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The phospholipid growth factors (PLGFs), including lysophosphatidic acid (LPA), have been implicated in corneal wound healing. PLGF concentrations and activities are elevated after corneal injury. Using real-time PCR, we quantified receptor mRNA levels in the healing rabbit cornea. In intact corneas, transcripts for S1P1, LPA1, and LPA3 receptor subtypes were detected, as was lipid phosphate phosphatase 1 (LPP1). After wounding, the trend for endothelium and keratocytes was for significant decreases in transcript numbers for the three receptor subtypes, whereas epithelial cells showed increase

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Yam, Seah, Yusoff, et al. "Characterization of Human Transition Zone Reveals a Putative Progenitor-Enriched Niche of Corneal Endothelium." Cells 8, no. 10 (2019): 1244. http://dx.doi.org/10.3390/cells8101244.

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: The corneal endothelium regulates corneal hydration to maintain the transparency of cornea. Lacking regenerative capacity, corneal endothelial cell loss due to aging and diseases can lead to corneal edema and vision loss. There is limited information on the existence of corneal endothelial progenitors. We conducted ultrastructural examinations and expression analyses on the human transition zone (TZ) at the posterior limbus of corneal periphery, to elucidate if the TZ harbored progenitor-like cells, and to reveal their niche characteristics. Within the narrow TZ (~190 μm width), the inner TZ

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Chi, Miaomiao, Bowei Yuan, Zijun Xie, and Jing Hong. "The Innovative Biomaterials and Technologies for Developing Corneal Endothelium Tissue Engineering Scaffolds: A Review and Prospect." Bioengineering 10, no. 11 (2023): 1284. http://dx.doi.org/10.3390/bioengineering10111284.

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Corneal transplantation is the only treatment for corneal endothelial blindness. However, there is an urgent need to find substitutes for corneal endothelium grafts due to the global shortage of donor corneas. An emerging research field focuses on the construction of scaffold-based corneal endothelium tissue engineering (CETE). Long-term success in CETE transplantation may be achieved by selecting the appropriate biomaterials as scaffolds of corneal endothelial cells and adding bioactive materials to promote cell activity. This article reviews the research progress of CETE biomaterials in the

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Tang, Q., and R. L. Hendricks. "Interferon gamma regulates platelet endothelial cell adhesion molecule 1 expression and neutrophil infiltration into herpes simplex virus-infected mouse corneas." Journal of Experimental Medicine 184, no. 4 (1996): 1435–47. http://dx.doi.org/10.1084/jem.184.4.1435.

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In a mouse model of herpes simplex virus (HSV) 1 corneal infection, tissue destruction results from a CD4+ T cell-mediated chronic inflammation, in which interleukin 2 and interferon (IFN) gamma are requisite inflammatory mediators and polymorphonuclear leukocytes (PMN) are the predominant infiltrating cells. In vivo neutralization of IFN-gamma relieved inflammation at least in part through a specific block of PMN extravasation into HSV-1-infected corneas. Intercellular adhesion molecule (ICAM) 1 and platelet endothelial cell adhesion molecule (PECAM) 1 were upregulated on the vascular endothe

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Singasandra, Sanjana Marijogaiah, Radhika Srinivasagupta Mohan, Dakshayini Mallaiah, and Shwetha Bennavara Venkataswamy. "A comparative study of endothelial cell loss in small incision cataract surgery versus phacoemulsification cataract surgery by using specular microscope at tertiary care ophthalmic centre in Bengaluru, Karnataka." IP International Journal of Ocular Oncology and Oculoplasty 7, no. 4 (2022): 399–405. http://dx.doi.org/10.18231/j.ijooo.2021.084.

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Most commonly performed surgical procedure in Ophthalmology is cataract extraction by MSICS and Phacoemulsification. It has always been associated with damage to endothelium of cornea, a layer which is vital for maintaining corneal transparency. The corneal endothelium regulates stromal hydration and maintains the transparency of the cornea by constantly removing the fluid out of the corneal stroma. The density of corneal endothelial cells and its integrity is an important determinant for corneal transparency. Post-operative corneal decompensation leading to reduced visual acuity can occur as

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Khodair, Aya A. I., Mouhamed Elsaadany Ghoraba, Rabab M. Elseht, and Dina Tadros. "Effect of intracameral injection of ceftazidime on corneal endothelium by specular microscope after uncomplicated phacoemulsification cataract surgery." Journal of the Egyptian Ophthalmological Society 117, no. 1 (2024): 59–62. http://dx.doi.org/10.4103/ejos.ejos_85_23.

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Background Loss of endothelial cells is inevitable after cataract surgery. Therefore, it is critical to protect the corneal endothelium during cataract surgery to reach good visual outcomes afterward. Maintenance of corneal clarity depends on the good function of healthy endothelial cells in addition to an intact pumping system intended to remove fluid from the corneal stoma. Purpose Our study aimed to assess changes in the corneal endothelium using specular microscopy, after intracameral injection of ceftazidime in uncomplicated phacoemulsification and compared with a placebo. The changes wer

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Ganekal, Sunil, and Varun Ganekal. "Anterior capsule sparing parsplana lensectomy in cataract with severely compromised corneal endothelium." Indian Journal of Clinical and Experimental Ophthalmology 8, no. 2 (2022): 224–27. http://dx.doi.org/10.18231/j.ijceo.2022.045.

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To evaluate the results of parsplana lensectomy in the management of cataract with severely compromised or abnormal corneal endothelium. Retrospective interventional case study comprised 22 patients (9 women, 13 men; 22 eyes) with severely abnormal corneal endothelium ut still had transparent cornea. Of which 14 had history of penetrating keratoplasty, 6 had history of trabeculectomy and 2 were diagnosed with Fuch’s endothelial dystrophy. Postoperative est corrected visual acuity (BCVA), mean density of corneal endothelial cell and other complications were analyzed. Mean follow-up period was 1

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Han, Jonathan, Nathan Wright, Sydni K. Davis Farhat, Florencia Yeh, and Robert E. Fintelmann. "Postcataract surgery gray-white lines of the corneal endothelium viewed with confocal microscopy and review of literature." Journal of Cataract & Refractive Surgery Online Case Reports 12, no. 2 (2024): e00120. http://dx.doi.org/10.1097/j.jcro.0000000000000120.

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Introduction: Cataract surgery rarely causes 1-day postoperative corneal endothelial changes visibly seen as patches or linear streaks. Previously described as “snailtracks,” these changes have been studied using slitlamp examination (SLE), scanning electron microscopy, and specular microscopy, but to the best of the authors' knowledge, it has yet to be analyzed using confocal microscopy. Patient and Clinical Findings: 3 patients who underwent cataract surgery through phacoemulsification with intraocular lens implant were found to have snailtracks on the corneal endothelium during postoperativ

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Yildirim, Timur Mert, Gerd U. Auffarth, Hyeck-Soo Son, Ramin Khoramnia, Donald John Munro, and Patrick R. Merz. "Dispersive viscosurgical devices demonstrate greater efficacy in protecting corneal endothelium in vitro." BMJ Open Ophthalmology 4, no. 1 (2019): e000227. http://dx.doi.org/10.1136/bmjophth-2018-000227.

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ObjectiveDuring phacoemulsification, the corneal endothelium is protected by an ophthalmic viscosurgical device (OVD). In this in vitro study, we assessed six different OVDs for their effectiveness in protecting the corneal endothelium.Methods and analysisPhacoemulsification was performed in cadaver eyes of young pigs. Five syringe units of six different OVDs were tested (Healon EndoCoat, Viscoat, Methylvisc, Healon, Healon GV, ProVisc). After surgery, the area of endothelium coated with OVD was determined in relation to the total endothelial surface. Additionally, an endothelial cell count wa

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Нijran, Namazova, and Sailova Gunel. "Changes in corneal endothelium and risk factors for surgical treatment of cataracts in the presence of pseudoexfoliation syndrome." Journal of Life Sciences and Biomedicine 2023, no. 1 (2023): 31–37. https://doi.org/10.5281/zenodo.8004184.

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Characteristic of pseudoexfoliative syndrome (PEX) corneal changes in the form of keratopathy which affects all layers of the cornea as the process progresses is a risk factor in cataract surgery especially in countries with a large elderly population. In cataract surgery, the corneal endothelium deserves increased attention because cells do not recover from their damage (Schlotzer-Schrehardt et al., 1993; Zheng, 2013). We studied corneal endothelial changes in 58 patients (58 eyes) with PEX from the perspective of the progression of the syndrome, risk factors of cataract surgery. From the sta

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Ostrovsky, D. S., S. A. Borzenok, B. E. Malyugin, O. P. Antonova, M. Kh Khubetsova, and T. Z. Kerimov. "Preparation and evaluation of a suspension of human corneal endothelial cells isolated from the eyes of cadaveric donors for transplantation in an <em>ex vivo</em> experiment." Russian Journal of Transplantology and Artificial Organs 26, no. 1 (2023): 103–12. http://dx.doi.org/10.15825/1995-1191-2024-1-103-112.

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Background. According to the World Health Organization, corneal diseases are one of the major causes of blindness globally. Endothelial dystrophy is one of the etiological factors leading to corneal diseases. The corneal endothelium is a monolayer of cells with virtually no mitotic activity. When the density of corneal endothelial cells falls below a critical threshold, the endothelium loses its ability to regulate corneal stromal hydration. This leads to corneal clouding and, consequently, to reduced visual acuity and quality of life of the patient. In this regard, various keratoplasty method

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Vilkelyte, Virginija, Polly Thompson, Maria Coelho, Małgorzata Woronkowicz, Piotr Skopinski, and Harry Roberts. "Challenges and Advances in Magnetic Nanoparticle-Guided Delivery of Cultured Human Corneal Endothelial Cells—A Review." Applied Sciences 14, no. 13 (2024): 5877. http://dx.doi.org/10.3390/app14135877.

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The cornea relies on a healthy endothelium to maintain transparency, and damage to endothelial cells can result in corneal oedema and vision loss. Current treatments, which often involve the use of donor corneas, face significant limitations due to a shortage of donor tissue. Although human corneal endothelial cells (HCECs) can be cultured and transplanted, their low attachment rates limit the effectiveness of these treatments. In this review, we examined studies that explore the use of magnetic nanoparticles (MNPs) to enhance the attachment of HCECs to the cornea. We evaluated the effectivene

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Fisenko, N. V. "Cornea: anatomical and functional features, new methods of in vivo diagnostics of abnormalities." Journal of Anatomy and Histopathology 11, no. 2 (2022): 78–86. http://dx.doi.org/10.18499/2225-7357-2022-11-2-78-86.

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The cornea is a highly organized, transparent part of fibrous tunic of an eyeball. It acts as the primary infectious and structural barrier of the eye. The cornea is the major refractive element of an adult eye. It consists of epithelium, Bowman's membrane, stroma, Descemet's membrane and endothelium. Although the normal human cornea is avascular, it is supplied via perilimbal blood vessels, the aqueous humor (AqH) and tear film. Afferent innervation to the cornea is provided by long ciliary nerves, which form subepithelial and subbasal nerve plexus. Epithelium is a stratified, non-keratinizin

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Reinstein Merjava, Stanislava, Jan Kossl, Ales Neuwirth, et al. "Presence of Protease Inhibitor 9 and Granzyme B in Healthy and Pathological Human Corneas." Biology 11, no. 5 (2022): 793. http://dx.doi.org/10.3390/biology11050793.

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The aim of this study was to find out whether protease inhibitor 9 (PI-9) and granzyme B (GrB) molecules that contribute to immune response and the immunological privilege of various tissues are expressed in healthy and pathological human corneas. Using cryosections, cell imprints of control corneoscleral discs, we showed that PI-9 was expressed particularly in the endothelium, the superficial and suprabasal epithelium of healthy corneas, limbus, and conjunctiva. GrB was localized in healthy corneal and conjunctival epithelium, while the endothelium showed weak immunostaining. The expression o

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Watsky, M. A. "Nonselective cation channel activation during wound healing in the corneal endothelium." American Journal of Physiology-Cell Physiology 268, no. 5 (1995): C1179—C1185. http://dx.doi.org/10.1152/ajpcell.1995.268.5.c1179.

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Rabbit corneas were injured by mechanical or thermal trauma. At several time points after wounding, corneal endothelial cells were isolated and their ion channels examined using standard and amphotericin perforated-patch whole cell patch-clamp configurations. Within 15-24 h after mechanical or thermal trauma, a nonselective cation current was observed in 79% of the cells examined that was not present in unwounded or sham-wounded corneas. By 73 h postwounding, the current was present in only 10% of the cells examined. The wound healing-induced current is outwardly rectifying, activates at depol

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Riley, Michael V., Barry S. Winkler, Catherine A. Starnes, and Margaret I. Peters. "Fluid and ion transport in corneal endothelium: insensitivity to modulators of Na+-K+-2Cl−cotransport." American Journal of Physiology-Cell Physiology 273, no. 5 (1997): C1480—C1486. http://dx.doi.org/10.1152/ajpcell.1997.273.5.c1480.

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The role of Na+-K+-2Cl−cotransport in ion and fluid transport of the corneal endothelium was examined by measuring changes in corneal hydration and uptake of86Rb by the endothelial cell layer. Isolated, intact rabbit corneas maintain normal hydration when they are superfused at the endothelial surface with bicarbonate ([Formula: see text])-Ringer solutions as a result of equilibrium between active ion and fluid transport out of the stromal tissue and leak of fluid into stromal tissue from the aqueous humor. Furosemide and bumetanide did not alter this equilibrium when they were added to the su

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Westin, Ida Maria, Andreas Viberg, Berit Byström, and Irina Golovleva. "Lower Fractions of TCF4 Transcripts Spanning over the CTG18.1 Trinucleotide Repeat in Human Corneal Endothelium." Genes 12, no. 12 (2021): 2006. http://dx.doi.org/10.3390/genes12122006.

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Fuchs’ endothelial corneal dystrophy (FECD) is a bilateral disease of the cornea caused by gradual loss of corneal endothelial cells. Late-onset FECD is strongly associated with the CTG18.1 trinucleotide repeat expansion in the Transcription Factor 4 gene (TCF4), which forms RNA nuclear foci in corneal endothelial cells. To date, 46 RefSeq transcripts of TCF4 are annotated by the National Center of Biotechnology information (NCBI), however the effect of the CTG18.1 expansion on expression of alternative TCF4 transcripts is not completely understood. To investigate this, we used droplet digital

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Siska, Siska, Gede Eka Wiratnaya, I. Made Bakta, et al. "The Role of Mesenchymal Stem Cells for Corneal Endothelial Regeneration: A Systematic Review." Rambam Maimonides Medical Journal 15, no. 4 (2024): e0017. http://dx.doi.org/10.5041/rmmj.10531.

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Objective: A single layer of tightly spaced cells, known as the endothelium, rests on the posterior side of the cornea. This endothelium regulates the stroma’s relative dehydration, which is essential for corneal clarity. Cell therapy is an innovative method being used to repair various corneal abnormalities. Mesenchymal stem cells (MSCs) are now one of the most significant types of stem cells scientists have studied. This study aimed to evaluate the role of MSCs for corneal endothelial regeneration. Methods: A systematic review was performed by searching for articles from reputable databases

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Nam, Ga Hee, Da Ran Kim, Young Chae Yoon, et al. "Development of an Instrument for Slit-lamp Examination of Donor Corneas in Preservation Medium." Journal of the Korean Ophthalmological Society 65, no. 2 (2024): 108–16. http://dx.doi.org/10.3341/jkos.2024.65.2.108.

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Purpose: To evaluate the effectiveness of an instrument devised for slit-lamp examination of donor corneas suspended in preservation medium.Methods: The study examined two donor corneas received at Yeouido St. Mary's Hospital in February 2023 and March 2023. The instrument has three main components: a plastic holder to hold the preservation medium bottle, a cube with a mirror for reflecting the slit beam, and a stand to attach the device to the slit-lamp. Using the instrument, the donor corneas were examined via slit-lamp: microscopy with the endothelium facing upward and downward. Specular mi

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Cerro-Tello, G., S. Benito-Martínez, C. Cacho-Navas, S. Barroso, G. de Rivas-Hidalgo, and J. Millán. "A unique F-actin and junctional organization that maintains the corneal endothelial barrier." IBJ Plus 1, s5 (2022): 6. http://dx.doi.org/10.24217/2531-0151.22v1s5.00006.

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Introduction: The corneal endothelium is responsible for the correct hydration of the corneal stroma and for an adequate transport through this tissue. Corneal endothelial layer has a particular organization to maintain its curved shape and function. The corneal endothelium has low proliferative capacity and form a monolayer that is subjected to an even mechanical tension coming from the positive pressure of the aqueous humor. Preserving their barrier function under suboptimal conditions, such as corneal pathologies, age and transplantation, is essential for maintaining corneal transparency. M

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Hsueh, Yi-Jen, Yaa-Jyuhn James Meir, Lung-Kun Yeh, et al. "Topical Ascorbic Acid Ameliorates Oxidative Stress-Induced Corneal Endothelial Damage via Suppression of Apoptosis and Autophagic Flux Blockage." Cells 9, no. 4 (2020): 943. http://dx.doi.org/10.3390/cells9040943.

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Compromised pumping function of the corneal endothelium, due to loss of endothelial cells, results in corneal edema and subsequent visual problems. Clinically and experimentally, oxidative stress may cause corneal endothelial decompensation after phacoemulsification. Additionally, in vitro and animal studies have demonstrated the protective effects of intraoperative infusion of ascorbic acid (AA). Here, we established a paraquat-induced cell damage model, in which paraquat induced reactive oxygen species (ROS) production and apoptosis in the B4G12 and ARPE-19 cell lines. We demonstrate that ox

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Malyugin, B. E., O. P. Antonova, and Z. R. Ebzeeva. "The comorbidity of keratoconus and Fuchs' endothelial corneal dystrophy (clinical cases)." Fyodorov journal of ophthalmic surgery, no. 3 (October 8, 2021): 95–103. http://dx.doi.org/10.25276/0235-4160-2021-3-95-103.

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Purpose. To describe the clinical cases and surgery results in patients with concomitant of keratoconus and Fuchs' corneal endothelial dystrophy. Material and methods. Retrospective analysis of 3 patients using different surgical techniques. All patients were female, they underwent diagnostic procedures including: keratotopography, optical coherence tomography of the anterior segment of the eye, Scheimpflug keratotomography, endothelial microscopy. First patient underwent penetrating keratoplasty (PKP), the second one – descemet stripping automated endothelial keratoplasty (DSEK), and the thir

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Parthasarathi, Priyadarshini, Venipriya, Justin Prashanth, and Hannah Ranjee Prasanth. "Atypical presentation of macular corneal dystrophy." Indian Journal of Clinical and Experimental Ophthalmology 8, no. 3 (2022): 428–30. http://dx.doi.org/10.18231/j.ijceo.2022.086.

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A 65-year old male patient presented to our ophthalmology OPD for regular check up. On examination visual acuity of the right eye was 6/24 improving to 6/12p with pinhole and left eye was 6/18 improving with pinhole 6/9. On examination of anterior segment both eyes cornea showed multiple white round deposits at deep posterior stroma and Descemet membrane – endothelium complex scattered circumferentially in the peripheral cornea and the central cornea clear and lens showed Immature cataract. Fundus examination was within normal limits. A differential diagnosis of stromal corneal dystrophy or en

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Winkler, B. S., M. V. Riley, M. I. Peters, and F. J. Williams. "Chloride is required for fluid transport by the rabbit corneal endothelium." American Journal of Physiology-Cell Physiology 262, no. 5 (1992): C1167—C1174. http://dx.doi.org/10.1152/ajpcell.1992.262.5.c1167.

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The role of chloride in fluid transport of the rabbit corneal endothelium was examined by measuring changes in corneal thickness following ion substitutions or addition of ion transport inhibitors in media superfusing the isolated tissue. Normal fluid transport is indicated by maintenance of constant thickness in a fresh cornea or thinning (deturgescence) of a preswollen deepithelialized cornea to its initial thickness at approximately 40 microns/h. These patterns are seen when tissues are superfused with HCO(3-)-Ringer containing 114 mM Cl-. When Cl- was substituted with gluconate, glucuronat

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Wieben, Eric D., Ross A. Aleff, Tommy A. Rinkoski, et al. "Comparison of TCF4 repeat expansion length in corneal endothelium and leukocytes of patients with Fuchs endothelial corneal dystrophy." PLOS ONE 16, no. 12 (2021): e0260837. http://dx.doi.org/10.1371/journal.pone.0260837.

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Expansion of CTG trinucleotide repeats (TNR) in the transcription factor 4 (TCF4) gene is highly associated with Fuchs Endothelial Corneal Dystrophy (FECD). Due to limitations in the availability of DNA from diseased corneal endothelium, sizing of CTG repeats in FECD patients has typically been determined using DNA samples isolated from peripheral blood leukocytes. However, it is non-feasible to extract enough DNA from surgically isolated FECD corneal endothelial tissue to determine repeat length based on current technology. To circumvent this issue, total RNA was isolated from FECD corneal en

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Ahmedov, A. K., T. Z. Kerimov, Kh D. Tonaeva, B. E. Malygin, and S. A. Borzenok. "Technology for obtaining an ultrathin posterior lamellar corneal graft at the Eye Tissue Bank." Russian Journal of Transplantology and Artificial Organs 22, no. 3 (2020): 167–73. http://dx.doi.org/10.15825/1995-1191-2020-3-167-173.

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Objective: to develop technologies for preoperative preparation of the posterior lamellar corneal graft based on our own formulation of the preservation medium for optimal dehydration of the donor cornea and a technique for cutting out an ultrathin flap using an optimized method at the Eye Tissue Bank. Materials methods. In a series of experimental studies, we obtained data on the hydration level of cadaveric donor corneas that were preserved in various solutions at different observation periods. Using 16 corneas, analytical weighing and pachymetry were performed via optical coherence tomograp

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Fontes, Bruno Machado, Francisco Bandeira, Ricardo Menon Nosé, and Patrick Frensel Tzelikis. "Essentials of the corneal endothelium for the cataract surgeon." Global Journal of Cataract Surgery and Research in Ophthalmology 1 (September 21, 2022): 64–80. http://dx.doi.org/10.25259/gjcsro_13_2022.

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The human eye is an optical system with two lenses in parallel, with complementary functions essential to vision: the cornea and the lens. There is an intimate relationship between these two structures, especially during cataract surgery when there is inevitably corneal endothelium injury at different severity levels. Every cataract surgeon should be aware of the functioning of the fragile corneal tissue, especially its noblest layer and responsible for corneal transparency: the endothelium. It is of paramount importance to be able to identify the different endothelial pathologies and local co

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Tkachenko, I. S., B. E. Malyugin, S. A. Borzenok, D. S. Ostrovskiy, and S. Y. Kalinnikova. "Experimental rationale for using a viscoprotection of the corneal endothelium on the graft formed with a femtosecond laser for the descemet stripping endothelial keratoplasty." Modern technologies in ophtalmology, no. 2 (June 15, 2021): 94–96. http://dx.doi.org/10.25276/2312-4911-2021-2-94-96.

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Purpose. To rationale experimentally the use of a viscoprotection of the corneal endothelium on the graft formed with a femtosecond laser for the descemet stripping endothelial keratoplasty. Material and methods. In our study, we used 12 pig's corneoscleral discs. The preservation time before the experiment averaged 12±4 hours. The corneas were divided into 2 groups. In the operating room, the graft was formed using an LDV Z8 femtosecond laser (Ziemer, Switzerland) from the endothelial side. Before applanation of the donor cornea and femtolaser head, a 1% solution of hydroxypropyl methylcellul

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Stoskopf, Michael K., Steven Zimmerman, Lawrence W. Hirst, and Richard Green. "Ocular anterior segment disease in northern fur seals." Journal of the American Veterinary Medical Association 187, no. 11 (1985): 1141–44. https://doi.org/10.2460/javma.1985.187.11.1141.

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SUMMARY Eyes of 1,716 wild northern fur seals (Callorhinus ursinus; 1,501 subadults and 215 adults) were examined for the prevalence of ocular anterior segment disease; 4.6% of the seals had clinically recognizable eye lesions. Six seals had bilateral eye lesions. Corneal scars were the most frequently observed lesion, followed by prominent lens sutures, cataracts, and iris depigmentations. Corneal edema, active corneal ulceration, or inflammation was not observed. Examination of normal corneas indicated an epithelial structure similar to that of the California sea lion, with 11 to 14 layers o

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