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1

Takizawa, T., and T. Saito. "Freeze-Fracture Enzyme Cytochemistry: Application of Enzyme Cytochemistry to Freeze-Fracture Cytochemistry." Journal of Electron Microscopy 45, no. 3 (1996): 242–46. http://dx.doi.org/10.1093/oxfordjournals.jmicro.a023440.

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2

Cuevas, P., J. A. Gutierrez-Diaz, D. Reimers, M. Dujovny, F. G. Diaz, and J. I. Ausman. "Intramembranous cytochemistry." Neurosurgery 20, no. 2 (1987): 243???8. http://dx.doi.org/10.1097/00006123-198702000-00008.

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3

Farhi, Diane C. "Haematological Cytochemistry." American Journal of Clinical Pathology 104, no. 2 (1995): 230.2–230. http://dx.doi.org/10.1093/ajcp/104.2.230a.

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4

Neame, PB, P. Soamboonsrup, GP Browman, et al. "Classifying acute leukemia by immunophenotyping: a combined FAB- immunologic classification of AML." Blood 68, no. 6 (1986): 1355–62. http://dx.doi.org/10.1182/blood.v68.6.1355.1355.

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Abstract A panel of commercially available monoclonal antibodies and five heteroantisera were used to distinguish and subtype 138 cases of acute leukemia (AL). The immunophenotype was compared with the French- American-British (FAB) classification obtained on the cases. The immunophenotype discriminated acute myelogenous leukemia (AML) from acute lymphoblastic leukemia (ALL) and recognized cases not distinguished by cytochemistry (22% of cases), mixed lineage phenotypes (13% of cases), and cases with separate populations of lymphoblasts and myeloblasts (one case). Using the immunologic panel a
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5

Neame, PB, P. Soamboonsrup, GP Browman, et al. "Classifying acute leukemia by immunophenotyping: a combined FAB- immunologic classification of AML." Blood 68, no. 6 (1986): 1355–62. http://dx.doi.org/10.1182/blood.v68.6.1355.bloodjournal6861355.

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A panel of commercially available monoclonal antibodies and five heteroantisera were used to distinguish and subtype 138 cases of acute leukemia (AL). The immunophenotype was compared with the French- American-British (FAB) classification obtained on the cases. The immunophenotype discriminated acute myelogenous leukemia (AML) from acute lymphoblastic leukemia (ALL) and recognized cases not distinguished by cytochemistry (22% of cases), mixed lineage phenotypes (13% of cases), and cases with separate populations of lymphoblasts and myeloblasts (one case). Using the immunologic panel and derive
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6

Takizawa, Toshihiro. "5′-Nucleotidase in Rat Photoreceptor Cells and Pigment Epithelial Cells Processed by Rapid-freezing Enzyme Cytochemistry." Journal of Histochemistry & Cytochemistry 46, no. 9 (1998): 1091–95. http://dx.doi.org/10.1177/002215549804600914.

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This report describes the subcellular distribution of 5′-nucleotidase (5′-NT) in rat photoreceptor cells and pigment epithelial cells processed by rapid-freeze enzyme cytochemistry. There was a striking difference in the ultrastructural localization of 5′-NT activity between rod outer segments after freeze-substitution fixation and conventional fixation. By rapid-freezing enzyme cytochemistry, 5′-NT activity was localized in the extradiscal space of intact nonvacuolated discs, whereas by conventional cytochemistry it was shown in the intradiscal space of artifactual vacuolated discs. In the fr
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7

Kitazawa, Sohei, Teruyuki Ohno, Ryuma Haraguchi, and Riko Kitazawa. "Histochemistry, Cytochemistry and Epigenetics." ACTA HISTOCHEMICA ET CYTOCHEMICA 55, no. 1 (2022): 1–7. http://dx.doi.org/10.1267/ahc.21-00095.

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8

Amiry-Moghaddam, Mahmood, and Ole Petter Ottersen. "Immunogold cytochemistry in neuroscience." Nature Neuroscience 16, no. 7 (2013): 798–804. http://dx.doi.org/10.1038/nn.3418.

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9

Roels, F., B. De Prest, and G. De Pestel. "Liver and chorion cytochemistry." Journal of Inherited Metabolic Disease 18, S1 (1995): 155–71. http://dx.doi.org/10.1007/bf00711437.

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10

Gossrau, R. "Cytochemistry of membrane proteases." Histochemical Journal 17, no. 7 (1985): 737–71. http://dx.doi.org/10.1007/bf01003312.

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11

Takizawa, Toshihiro, Takuma Saito, and John M. Robinson. "Freeze-fracture Cytochemistry: A New Method Combining Immunocytochemistry and Enzyme Cytochemistry on Replicas." Journal of Histochemistry & Cytochemistry 46, no. 1 (1998): 11–17. http://dx.doi.org/10.1177/002215549804600103.

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We describe a new freeze-fracture cytochemical technique consisting of combined immunocytochemistry and enzyme cytochemistry. This technique reveals the relationship between molecules in biological membranes by double labeling with two different cytochemical markers (i.e., immunogold probes and cerium). In this method, antigens were detected with specific primary antibodies and appropriate secondary immunoprobes. Subsequently, alkaline phosphatase activity was detected with cerium as the capture agent on the same replicas. Octyl-glucoside (OG) digestion before the cytochemical reactions was cr
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12

Tamaki, H., and S. Yamashina. "Improved method for post-embedding cytochemistry using reduced osmium and LR white resin." Journal of Histochemistry & Cytochemistry 42, no. 9 (1994): 1285–93. http://dx.doi.org/10.1177/42.9.8064136.

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We established an improved method for post-embedding cytochemistry by which highly specific cytochemical reactions on excellent cellular ultrastructures are possible. The method is a combination of post-fixation in potassium ferrocyanide-reduced OsO4 and embedment in acrylic-based LR White resin. It permits both immuno- and lectin-gold cytochemistry with fine ultrastructures comparable to those obtained by conventionally osmicated and epoxy-embedded tissues. Fixation with reduced osmium appeared to contribute to the preservation of immunoreactivity and membranous structures. By this method, th
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13

Kan, Frederick W. K. "Applications of freeze-fracture cytochemistry as applied to the study of tissues and cells." Proceedings, annual meeting, Electron Microscopy Society of America 45 (August 1987): 1006–9. http://dx.doi.org/10.1017/s0424820100129280.

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The applications of freeze-fracture cytochemistry to the study of tissues and cells are described. Two approaches will be presented: label-fracture and fracture-label. In both techniques, colloidal gold probe is used as the marker system. The use of cytochemistry in combination with freeze-fracture for the study of distribution of receptor sites, antigens and glyco- conjugates on freeze-fractured plasma membranes as well as on fractured intracellular organelles is illustrated respectively by the two models described below. A variant of the fracture-label technique, Backscattered Electron Imagi
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14

Bendix-Hansen, Knud. "ENZYME CYTOCHEMISTRY OF NEUTROPHIL GRANULOCYTES." British Journal of Haematology 65, no. 2 (1987): 127–29. http://dx.doi.org/10.1111/j.1365-2141.1987.tb02253.x.

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15

Fitzsimrnons, E. I. "Leukaemia Cytochemistry-Principles and Practice." Histopathology 16, no. 3 (1990): 313–14. http://dx.doi.org/10.1111/j.1365-2559.1990.tb01127.x.

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16

Mizuhira, V., and H. Hasegawa. "Microwave fixation method for cytochemistry." European Journal of Morphology 34, no. 5 (1996): 385–92. http://dx.doi.org/10.1076/ejom.34.5.385.13055.

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17

Weis, Kitren G., Karin R. Jacobsen, and Judith A. Jernstedt. "Cytochemistry of developing cotton fibers:." Field Crops Research 62, no. 2-3 (1999): 107–17. http://dx.doi.org/10.1016/s0378-4290(99)00004-0.

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18

Nepi, M., and G. G. Franchi. "Cytochemistry of mature angiosperm pollen." Plant Systematics and Evolution 222, no. 1-4 (2000): 45–62. http://dx.doi.org/10.1007/bf00984095.

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19

Coulton, Gary. "Are histochemistry and cytochemistry 'Omics'?" Histochemical Journal 35, no. 6 (2004): 603–13. http://dx.doi.org/10.1007/s10735-004-2193-7.

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20

Chatterjee, Tathagata, Manoranjan Mahapatra, Hara P. Pati, et al. "Use of Transmission Electron Microscopy in Diagnosis of Acute Leukemias: A Prospective Study of Fifty Cases." Blood 106, no. 11 (2005): 4509. http://dx.doi.org/10.1182/blood.v106.11.4509.4509.

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Abstract Ultrastructural studies have contributed substantially to the understanding of the cellular morphology of the acute leukemias. We present here 50 cases (aged 3-55 years, M: F-32: 18) of acute leukemias, which were studied for morphology, conventional cyto chemistry, immunophenotyping, and transmission electron microscopy (TEM) including ultrastructural cytochemistry using myeloperoxidase (MPO) and platelet peroxidase activity. TEM morphology using ultrastructural cytochemistry of MPO helped diagnose three cases of acute myeloid leukemia with minimal myeloid differentiation (AML M0). T
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21

Pargney, Jean-Claude. "Essais de caractérisation cytochimique des structures de l'interface au niveau du réseau de Hartig dans l'association ectomycorhizienne entre la truffe (Tuber melanosporum) et le noisetier (Corylus avellana)." Canadian Journal of Botany 68, no. 12 (1990): 2722–28. http://dx.doi.org/10.1139/b90-345.

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The interface established between Tuber melanosporum and Corylus avellana was studied cytochemically (PATAg test, Swift's reaction, wheat germ agglutinin – colloidal gold labelling) to characterize cell wall and matrix components. By combining ultrastructural cytochemistry and selective extractions of polysaccharides by various solvents (EDTA, dimethyl sulfoxide, methylamine) or enzymes (pectinase, cellulase, cytohelicase), some ultrastructural features were made evident. Ultrastructural cytochemical tests demonstrate different domains in the matrix. Cell wall and matrix components are similar
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22

Willis, Clinton, Johanna Nyffeler, and Joshua Harrill. "Phenotypic Profiling of Reference Chemicals across Biologically Diverse Cell Types Using the Cell Painting Assay." SLAS DISCOVERY: Advancing the Science of Drug Discovery 25, no. 7 (2020): 755–69. http://dx.doi.org/10.1177/2472555220928004.

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Cell Painting is a high-throughput phenotypic profiling assay that uses fluorescent cytochemistry to visualize a variety of organelles and high-content imaging to derive a large number of morphological features at the single-cell level. Most Cell Painting studies have used the U-2 OS cell line for chemical or functional genomics screening. The Cell Painting assay can be used with many other human-derived cell types, given that the assay is based on the use of fluoroprobes that label organelles that are present in most (if not all) human cells. Questions remain, however, regarding the optimizat
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23

Robinson, J. M., and M. J. Karnovsky. "Rapid-freezing cytochemistry: preservation of tubular lysosomes and enzyme activity." Journal of Histochemistry & Cytochemistry 39, no. 6 (1991): 787–92. http://dx.doi.org/10.1177/39.6.2033237.

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We show that tubular structures present in phorbol ester-stimulated macrophages are sensitive to commonly used chemical fixatives (i.e., they usually become fragmented during fixation). These structures are well preserved in macrophages that are physically fixed by rapid-freezing and subsequent freeze-substitution in osmium-acetone. We have developed methods that combine rapid-freezing, freeze-substitution, and enzyme cytochemistry for preservation of these tubular structures and for detection of endocytosed material (i.e., horseradish peroxidase). This method of rapid-freeze cytochemistry may
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24

Rendayan, Moise. "Facts and artifacts in colloidal gold postembedding cytochemistry." Proceedings, annual meeting, Electron Microscopy Society of America 44 (August 1986): 44–47. http://dx.doi.org/10.1017/s0424820100141962.

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The colloidal gold marker was introduced in immunocytochemistry by Faulk and Taylor, in 1971, for the ultrastructural localization of surface antigens. Since then, application of this marker in light and electron microscopy has been growing rapidly. In particular, it has been applied for postembedding labeling of intracellular binding sites and its use has been extended to the various fields of cytochemistry: immunocytochemistry (protein A-gold), IgG-gold), enzyme-cytochemistry and lectincytochemistry. Several reviews have been recently published on colloidal gold labeling techniques and we re
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25

Arshad A., Abu, Narmatha M., and Ulaganathan S. "HEMATOLOGICAL AND CLINICAL EVALUATION OF LEUKEMIAS, USING CYTOCHEMICAL STAINS AND IMMUNOPHENOTYPING." International Journal of Advanced Research 10, no. 09 (2022): 973–78. http://dx.doi.org/10.21474/ijar01/15444.

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Background: Leukemias are abnormal proliferation of hematopoietic cells, causing progressive infiltration of the marrow. It is the eleventh most common cancer in the world, and increasingly found now in developing countries. Two widely used classifications are used now, the FAB, and the WHO classification, which has got supplanted now, with increasing knowledge on cytomorphology and cytogenetics. This study, attempts to evaluate the role of cytochemistry as a cost-effective tool, in the various types of leukemias, and the role of immunophenotyping in a select few cases. Aim:- The main aim of t
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26

MASUZAWA, Toshio, Toshiko OHTA, and Fumiaki SATO. "Enzyme Cytochemistry of Ventricular Choroid Plexus." Neurologia medico-chirurgica 25, no. 11 (1985): 881–85. http://dx.doi.org/10.2176/nmc.25.881.

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27

Kurosawa, Hidemitsu, Mitsuoki Eguchi, Hitoshi Sakakibara, Hiroshi Takahashi, and Toshiharu Furukawa. "Ultrastructural Cytochemistry of Congenital Basophilic Leukemia." Journal of Pediatric Hematology/Oncology 9, no. 1 (1987): 27–32. http://dx.doi.org/10.1097/00043426-198721000-00006.

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28

Glick, D. "Fifty years with histochemistry and cytochemistry." Journal of Histochemistry & Cytochemistry 33, no. 7 (1985): 720–28. http://dx.doi.org/10.1177/33.7.2409132.

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29

Vrčić, Hrvoje, Božidar Horvat, and Ivan Damjanov. "Lectin Cytochemistry of Mouse Vaginal Smears." Gynecologic and Obstetric Investigation 33, no. 2 (1992): 69–74. http://dx.doi.org/10.1159/000294851.

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30

Ishiyama, Gail, Ivan A. Lopez, Ali R. Sepahdari, and Akira Ishiyama. "Meniere's disease: histopathology, cytochemistry, and imaging." Annals of the New York Academy of Sciences 1343, no. 1 (2015): 49–57. http://dx.doi.org/10.1111/nyas.12699.

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31

Yamashita, Shuji. "Histochemistry and Cytochemistry of Nuclear Receptors." Progress in Histochemistry and Cytochemistry 36, no. 2 (2001): 91–176. http://dx.doi.org/10.1016/s0079-6336(01)80004-8.

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32

Holland, G. R. "Nociceptor sprouting and cytochemistry in injury." Pathophysiology 5 (June 1998): 164. http://dx.doi.org/10.1016/s0928-4680(98)80929-2.

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33

da Silva, Pedro Pinto. "Molecular cytochemistry of freeze-fractured cells." Proceedings, annual meeting, Electron Microscopy Society of America 44 (August 1986): 894–97. http://dx.doi.org/10.1017/s0424820100145807.

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I will describe four approaches that combine cytochemistry with freeze-fracture: 1) FREEZE-ETCHING; 2) FRACTURE-LABEL; 3) FRACTURE-PERMEATION; and 4) LABEL-FRACTURE. These techniques, in particular fracture-label, involve delicate points of interpretation and numerous validating controls. In the publications listed at the end, these issues have been addressed in detail.1. FREEZE-ETCHING. I developed freeze-etching as a cytochemical approach to prove that membranes were split by freeze-fracture and to show that biological membranes were comprised of a bilayer membrane continuum interrupted by i
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34

Li, Chin-Yang, and Lung T. Yam. "Cytochemistry and Immunochemistry in Hematologic Diagnoses." Hematology/Oncology Clinics of North America 8, no. 4 (1994): 665–81. http://dx.doi.org/10.1016/s0889-8588(18)30153-9.

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35

Van Noorden, Cornelis J. F. "Molecular probes in histochemistry and cytochemistry." Acta Histochemica 100, no. 4 (1998): 337. http://dx.doi.org/10.1016/s0065-1281(98)80030-5.

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36

Andersson Forsman, C. "Freeze-fracture cytochemistry of sympathetic ganglia." Histochemistry 82, no. 3 (1985): 209–18. http://dx.doi.org/10.1007/bf00501397.

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37

Ploem, J. S., A. M. J. van Driel-Kulker, L. Goyarts-Veldstra, J. J. Ploem-Zaaijer, N. P. Verwoerd, and M. van der Zwan. "Image analysis combined with quantitative cytochemistry." Histochemistry 84, no. 4-6 (1986): 549–55. http://dx.doi.org/10.1007/bf00482990.

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38

Fujimoto, K., K. S. Ogawa, and K. Ogawa. "Gastric K+-stimulated p-nitrophenylphosphatase cytochemistry." Histochemistry 84, no. 4-6 (1986): 600–608. http://dx.doi.org/10.1007/bf00482998.

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39

VANMEIR, F., and D. SCHEUERMANN. "Catalase cytochemistry of interalveolar septal cells." Cell Biology International Reports 14 (September 1990): 212. http://dx.doi.org/10.1016/0309-1651(90)90949-y.

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40

Moore, Michael N. "Lysosomal cytochemistry in marine environmental monitoring." Histochemical Journal 22, no. 4 (1990): 187–91. http://dx.doi.org/10.1007/bf02386003.

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41

Sanna, Pietro Paolo, Gustav F. Jirikowski, Gail A. Lewandowski, and Floyd E. Bloom. "Applications of DAPI Cytochemistry to Neurobiology." Biotechnic & Histochemistry 67, no. 6 (1992): 346–50. http://dx.doi.org/10.3109/10520299209110047.

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42

Gahan, P. B. "Quantitative enzyme cytochemistry in plant biotechnology." Phytochemical Analysis 2, no. 3 (1991): 97–106. http://dx.doi.org/10.1002/pca.2800020302.

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43

Beesley, J. E. "Immunolabelling and electron microscopy in cytochemistry." Current Opinion in Immunology 2, no. 6 (1989): 927–31. http://dx.doi.org/10.1016/0952-7915(89)90180-5.

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44

Severs, Nicholas J. "Freeze-fracture cytochemistry: Review of methods." Journal of Electron Microscopy Technique 13, no. 3 (1989): 175–203. http://dx.doi.org/10.1002/jemt.1060130306.

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45

Invernizzi, R., R. Nano, O. Perugini, et al. "Tetrahydrofolate dehydrogenase cytochemistry in acute lymphoblastic leukemia." European Journal of Haematology 41, no. 2 (2009): 109–14. http://dx.doi.org/10.1111/j.1600-0609.1988.tb00879.x.

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46

Derenzini, M., M. Thiry, and G. Goessens. "Ultrastructural cytochemistry of the mammalian cell nucleolus." Journal of Histochemistry & Cytochemistry 38, no. 9 (1990): 1237–56. http://dx.doi.org/10.1177/38.9.2201735.

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In the present review on the organization of the mammalian cell nucleolus, we report and discuss data obtained during the past 10 years by means of cytochemical and immunocytochemical ultrastructural techniques. Particular emphasis is placed on the following topics: location of the nucleolus organizer regions in interphasic nucleolar components, structure of nucleolar chromatin in situ, and the structure-function relationship of the nucleolar components. The cytochemical and immunocytochemical results are compared and the concordant data are stressed for each topic.
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47

Maraldi, N. M., N. Zini, S. Squarzoni, R. Del Coco, P. Sabatelli, and F. A. Manzoli. "Intranuclear localization of phospholipids by ultrastructural cytochemistry." Journal of Histochemistry & Cytochemistry 40, no. 9 (1992): 1383–92. http://dx.doi.org/10.1177/40.9.1506675.

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The presence of phospholipids within the interphase nucleus and in isolated chromatin, previously demonstrated by analytical biochemical methods, has been only rarely documented by cytochemical procedures, especially at the ultrastructural level. By means of a gold-conjugated phospholipase technique, we investigated the fine localization of endogenous phospholipids in the different nuclear domains in rat pancreas and in cell cultures. To reduce possible removal or displacement of phospholipids, different specimen preparation procedures such as cryofixation, cryosectioning, and freeze-fracturin
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48

Burry, Richard W., and Denis G. Baskin. "The Online Journal of Histochemistry and Cytochemistry." Journal of Histochemistry & Cytochemistry 46, no. 1 (1998): 1–2. http://dx.doi.org/10.1177/002215549804600101.

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49

Bojović-Cvetić, Dubravka, and Radmila Vujičić. "Polysaccharide cytochemistry in maturing Aspergillus flavus sclerotia." Transactions of the British Mycological Society 91, no. 4 (1988): 619–24. http://dx.doi.org/10.1016/s0007-1536(88)80036-6.

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50

Stussi-Garaud, Christiane, and Odette Rohfritsch. "Methods in Plant Electron Microscopy and Cytochemistry." Plant Science 160, no. 4 (2001): 753–54. http://dx.doi.org/10.1016/s0168-9452(00)00434-9.

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