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Rozprawy doktorskie na temat „De novo leaf transcriptome”

Autor: Grafiati
Data publikacji: 3 czerwca 2025
Data aktualizacji: 31 lipca 2025

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1

Der, Joshua, Michael Barker, Norman Wickett, Claude dePamphilis, and Paul Wolf. "De novo characterization of the gametophyte transcriptome in bracken fern, Pteridium aquilinum." BioMed Central, 2011. http://hdl.handle.net/10150/610016.

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BACKGROUND:Because of their phylogenetic position and unique characteristics of their biology and life cycle, ferns represent an important lineage for studying the evolution of land plants. Large and complex genomes in ferns combined with the absence of economically important species have been a barrier to the development of genomic resources. However, high throughput sequencing technologies are now being widely applied to non-model species. We leveraged the Roche 454 GS-FLX Titanium pyrosequencing platform in sequencing the gametophyte transcriptome of bracken fern (Pteridium aquilinum) to de
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Lafond, Lapalme Joël. "Étude et décontamination du transcriptome de novo du nématode doré Globodera rostochiensis." Mémoire, Université de Sherbrooke, 2016. http://hdl.handle.net/11143/8882.

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Le nématode doré, Globodera rostochiensis, est un nématode phytoparasite qui peut infecter des plantes agricoles telles la pomme de terre, la tomate et l’aubergine. En raison des pertes de rendement considérables associées à cet organisme, il est justifiable de quarantaine dans plusieurs pays, dont le Canada. Les kystes du nématode doré protègent les œufs qu’ils contiennent, leur permettant de survivre (en état de dormance) jusqu’à 20 ans dans le sol. L’éclosion des œufs n’aura lieu qu’en présence d’exsudats racinaires d’une plante hôte compatible à proximité. Malheureusement, très peu de conn
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Veselovská, Lenka. "Defining the oocyte transcriptome and its relationship to de novo DNA methylation." Thesis, University of Cambridge, 2015. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.709331.

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Meng, Arnaud. "Étude de la symbiose dans le plancton marin par une approche transcriptome et méta-transcriptome." Thesis, Paris 6, 2017. http://www.theses.fr/2017PA066478/document.

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Les relations symbiotiques entre organismes sont essentielles pour l’évolution de la bio- diversité et le fonctionnement des écosystèmes. En milieu terrestre ou en milieu marin benthique les symbioses sont assez bien décrites. Si dans le plancton marin, les relations entre hôtes hétérotrophes et symbiontes photosynthétiques sont des phénomènes observés dès le 19ème siècle, les mécanismes fonctionnels qui régissent ces symbioses restent largement inconnus. C’est le cas de la symbiose entre certaines espèces de radiolaires et leurs symbiontes dinoflagellés. Il s’agit d’un mode
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Meng, Arnaud. "Étude de la symbiose dans le plancton marin par une approche transcriptome et méta-transcriptome." Electronic Thesis or Diss., Paris 6, 2017. http://www.theses.fr/2017PA066478.

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Les relations symbiotiques entre organismes sont essentielles pour l’évolution de la bio- diversité et le fonctionnement des écosystèmes. En milieu terrestre ou en milieu marin benthique les symbioses sont assez bien décrites. Si dans le plancton marin, les relations entre hôtes hétérotrophes et symbiontes photosynthétiques sont des phénomènes observés dès le 19ème siècle, les mécanismes fonctionnels qui régissent ces symbioses restent largement inconnus. C’est le cas de la symbiose entre certaines espèces de radiolaires et leurs symbiontes dinoflagellés. Il s’agit d’un mode
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Tassone, Erica E., Gina Zastrow-Hayes, John Mathis, et al. "Sequencing, de novo assembly and annotation of a pink bollworm larval midgut transcriptome." BioMed Central, 2016. http://hdl.handle.net/10150/618722.

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Background: The pink bollworm Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae) is one of the world's most important pests of cotton. Insecticide sprays and transgenic cotton producing toxins of the bacterium Bacillus thuringiensis (Bt) are currently used to manage this pest. Bt toxins kill susceptible insects by specifically binding to and destroying midgut cells, but they are not toxic to most other organisms. Pink bollworm is useful as a model for understanding insect responses to Bt toxins, yet advances in understanding at the molecular level have been limited because basic ge
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Khan, Hamza. "De novo annotation of non-model organisms using whole genome and transcriptome shotgun sequencing." Thesis, University of British Columbia, 2016. http://hdl.handle.net/2429/60152.

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Fonseca, Natália Risso. "Etiology and de novo transcriptome analysis of the powdery mildew pathogen on Eucalyptus in Brazil." Universidade Federal de Viçosa, 2016. http://www.locus.ufv.br/handle/123456789/10763.

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Submitted by Reginaldo Soares de Freitas (reginaldo.freitas@ufv.br) on 2017-06-20T17:30:34Z No. of bitstreams: 1 texto completo.pdf: 1425926 bytes, checksum: ee624a74ef4a36d197d9316ae8fe4072 (MD5)<br>Made available in DSpace on 2017-06-20T17:30:34Z (GMT). No. of bitstreams: 1 texto completo.pdf: 1425926 bytes, checksum: ee624a74ef4a36d197d9316ae8fe4072 (MD5) Previous issue date: 2016-08-25<br>Conselho Nacional de Desenvolvimento Científico e Tecnológico<br>Oídio do eucalipto é uma importante doença que ocorre principalmente em casas de vegetação e minijardins clonais protegidos de eucali
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Pandreka, A. "De novo sequencing and analysis of transcriptome from azadirachta indica to characterize the genes involved in limonoid biosynthesis." Thesis(Ph.D.), CSIR-Institute of Genomics and Integrative Biology, New Delhi, 2018. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/5846.

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Smith, Emily. "De novo Transcriptome Analysis of the Marine Sponge Cinachyrella spp: A Potential Model Organism for Oil and Dispersant Ecotoxicology." NSUWorks, 2013. http://nsuworks.nova.edu/occ_stuetd/141.

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In order to study the potential effects of an oil spill on coral reef organisms, the marine sponge, Cinachyrella spp. was investigated. In this study, Cinachyrella spp. was placed in a closed aquaculture system and exposed to sub-lethal water-accommodated fractions (WAFs) of Macondo crude oil and chemically-enhanced water accommodated fractions (CE-WAFs) of the dispersant, Corexit 9500, over a 24-hour time course, in order to model the BP Deepwater Horizon oil spill and oil spill sponge response. Illumina RNA sequencing and gene expression analysis utilizing hierarchical clustering, principal
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Wimberley, James. "De novo Sequencing and Analysis of Salvia hispanica Transcriptome and Identification of Genes Involved in the Biosynthesis of Secondary Metabolites." Chapman University Digital Commons, 2019. https://digitalcommons.chapman.edu/cads_theses/5.

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Salvia hispanica L. (commonly known as chia) is gaining popularity worldwide and specially in US as a healthy oil and food supplement for human and animal consumption due to its favorable oil composition, and high protein, fiber, and antioxidant contents. Despite these benefits and its growing public demand, very limited gene sequence information is currently available in public databases. In this project, we generated 90 million high quality 150 bp paired-end sequences from the chia leaf and root tissues. The sequences were de novo assembled into 103,367 contigs with average length of 1,445 b
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Moreira, Raulzito Fernandes. "AlteraÃÃo no perfil de expressÃo dos genes de folhas de Cajueiro CCP76 (Anacardium occidentale L.) em resposta ao estresse salino." Universidade Federal do CearÃ, 2014. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=12901.

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CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior<br>O estresse salino à apontado como tendo impactos negativos sobre a produÃÃo de cultivares de interesse comercial. Nos Ãltimos anos os estudos tÃm focado na pesquisa e no entendimento dos mecanismos utilizados pelas plantas que toleram esse tipo de estresse naturalmente. Dentre as espÃcies que toleram ao estresse salino encontra-se Anacardium occidentale L. ou popularmente conhecida como Cajueiro. Trabalhos na literatura jà descrevem mecanismos que esse cultivar pode utilizar para suportar a condiÃÃo de estresse. Contudo, estudos ad
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Esquivel, Palma Carlos Josue. "Effects of Blood Feeding on The Transcriptome of The Malpighian Tubules in The Asian Tiger Mosquito Aedes albopictus." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1429619765.

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14

Sturtevant, Drew. "Compartmentalization of Jojoba Seed Lipid Metabolites." Thesis, University of North Texas, 2018. https://digital.library.unt.edu/ark:/67531/metadc1404575/.

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Seeds from the desert shrub Simmondsia chinensis (jojoba) are one of the only known natural plant sources to store a majority of its oil in the form of liquid wax esters (WE) instead of triacylglycerols (TAGs) and these oils account for ~55% of the seed weight. Jojoba oil is highly valued as cosmetic additives and mechanical lubricants, yet despite its value much is still unknown about its neutral lipid biosynthetic pathways and lipid droplet packaging machinery. Here, we have used a multi-"omics" approach to study how spatial differences in lipid metabolites, gene expression, and lipid drople
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15

Valderrama, Escallon Eugenio. "Explaining the differences in African and Neotropical species richness by comparing diversification rates in Renealmia L.f. (Zingiberaceae)." Thesis, University of Edinburgh, 2016. http://hdl.handle.net/1842/25791.

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The well-known high species richness of the tropical forests is not uniform through its different regions; Africa is species-poor when compared to Southeast Asia and the Neotropical region. One of the hypotheses for differences between the richness in the Neotropics and Africa points to the importance of recent speciation in the Neotropics. This is considered in particular in Andean-centred taxa that probably diversified in response to the opportunities for speciation offered by the final uplift of the tropical Andes (during the past c. 25 million years [Ma] to the present, with higher rates o
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16

Amin, Shorash. "Investigating genetic, gene expression and proteomic changes over temperature gradients in intertidal Nerita species." Thesis, Queensland University of Technology, 2018. https://eprints.qut.edu.au/121483/2/Shorash_Amin_Thesis.pdf.

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This project undertook a comparative study to investigate how marine snails respond to acute temperature stress conditions that are expected as a result of global climate change. It was found that each snail species employed a different strategy to cope with temperature stress, but not all strategies were successful under extreme stress conditions. This information can be used to predict which species will be most susceptible to future climate changes and therefore more efficiently manage these species.
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Stock, Magdalena [Verfasser], Wilhelm [Akademischer Betreuer] Boland, Reinhard [Akademischer Betreuer] Guthke, and Karl [Akademischer Betreuer] Schmid. "Expression analysis and phylogeny studies based on de novo assembled transcript libraries of leaf beetle larvae / Magdalena Stock. Gutachter: Wilhelm Boland ; Reinhard Guthke ; Karl Schmid." Jena : Thüringer Universitäts- und Landesbibliothek Jena, 2014. http://d-nb.info/1058857967/34.

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18

Campagna, Davide. "Gene expression study in the non-model organism Botryllus schlosseri through SOLiD RNA-seqs." Doctoral thesis, Università degli studi di Padova, 2013. http://hdl.handle.net/11577/3423362.

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Botryllus schlosseri is a colonial ascidian widespread in temperate, shallow seas of the world. The organism is widely used for the study, in an evolutionary perspective, of a variety of biological processes ranging from sexual and asexual reproduction to regeneration, allorecognition and immune responses. However, despite its importance as model organism, no sequenced genome is available today. We undertook the analysis of the transcriptome of B. schlosseri in various colonial developmental phases and during tunic regeneration. The asexual reproduction by continuous palleal budding and the va
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19

Kim, Gunjune. "Massive Exchange of mRNA between a Parasitic Plant and its Hosts." Diss., Virginia Tech, 2014. http://hdl.handle.net/10919/50509.

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Cuscuta pentagona is an obligate parasitic plant that hinders production of crops throughout the world. Parasitic plants have unique morphological and physiological features, the most prominent being the haustorium, a specialized organ that functions to connect them with their host's vascular system. The Cuscuta haustorium is remarkable in that it enables mRNA movement to occur between hosts and parasite, but little is known about the mechanisms regulating cross-species mRNA transfer or its biological significance to the parasite. These questions were addressed with genomics approaches tha
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20

Souza, Vinicius Carius de. "Análise do transcriptoma de Lippia alba (Mill.) N.E.Br. (Verbenaceae) por RNAseq visando a identificação de enzimas terpeno sintases." Universidade Federal de Juiz de Fora (UFJF), 2016. https://repositorio.ufjf.br/jspui/handle/ufjf/5286.

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NEGRINI, Pietro. "Molecular and Bioinformatic Analysis of the Circadian Clock in Phreatichthys andruzzii." Doctoral thesis, Università degli studi di Ferrara, 2015. http://hdl.handle.net/11392/2389077.

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Daily cycles of light and temperature imposed by the rotation of the Earth on its axis have had a major impact on the evolution of all living organisms. Fascinating demonstrations of this fact can be seen in extreme environments such as caves or in the deep sea, where some species have evolved in complete isolation from daily light-dark cycles for millions of years, sharing a range of striking physical characters acquired by convergent evolution such as loss of eyes and pigmentation. One fundamental issue is to investigate whether these “hypogean” species still retain a functional circadian cl
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22

Ambu, Ali Aisha. "Morphological and functional aspects of feeding in the freshwater fish louse Argulus foliaceus (Linnaeus, 1758)." Thesis, University of Stirling, 2017. http://hdl.handle.net/1893/26045.

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Argulus foliaceus (Linnaeus, 1758) is a member of the branchiuran family Argulidae and has a worldwide distribution, causing major economic impacts for freshwater aquacultured fish species worldwide. In the UK, it has economic impacts for both aquaculture and sports fishing industries. Previous studies observed haemorrhagic and inflammatory responses after Argulus infection, which has been taken to support the idea that the parasite secretes chemicals during the feeding process to assist with the ingestion of blood and epithelial tissue. The present study suggests that the blood-feeding ectopa
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Guiguet, Antoine. "Origine évolutive et bases moléculaires du mode de vie galligène chez les Gracillariidae." Thesis, Tours, 2019. http://www.theses.fr/2019TOUR4005.

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L’objectif de ma thèse visait à étudier les processus évolutifs ayant conduit à l’évolution du mode de vie galligène et à rechercher des effecteurs impliqués dans l’induction des galles dans la famille des Gracillariidae (Lepidoptera) avec un accent particulier sur deux espèces, Borboryctis euryae et Caloptilia cecidophora. Nous avons ainsi démontré que ces deux espèces présentent la particularité de posséder un mode de vie intermédiaire entre mineur de feuille et inducteur de galle. Le tissu prolifératif présent dans la mine de B. euryae s’apparente en effet à une galle et les larves de C. ce
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Chen, Yi-Ying, and 陳怡穎. "OrchidWeb: A Comprehensive Web Server for De Novo Orchid Transcriptome." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/18379724138929880224.

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碩士<br>國立中興大學<br>基因體暨生物資訊學研究所<br>100<br>Orchids belong to one of the largest, most diverse flowering plant families. There are over 800 genera of Orchids in the world and more than 25,000 species as well. Orchids are the high value crops in Taiwan. Nowadays the best production is the Phalaenopsis. Phalaenopsis is a beautiful flower in the world, and many people love it. Until now it has a lot of studies for planting technology and plant breeding. The orchid research includes functional genomics, Expressed sequence tag (ESTs), Complementary DNA (cDNA) researches with the databases that helps mol
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Lu, Kuan-Chieh, and 呂冠杰. "De novo assembly and characterization of the transcriptome of Neocaridina denticulata." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/78432603059346291866.

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碩士<br>國立交通大學<br>生物資訊及系統生物研究所<br>103<br>Neocaridina denticulata, known as black cherry shrimps, has been as test subjects in Taiwan for water toxicity texting experiments for its tribute tendency to response to a variety of chronic or acute toxicity. However, both the genomic and transcriptomic information of such an organism is limited. To further improve the current experiment design and elucidate the mechanism of Neocaridina denticulata’s response to pollutants, we reconstructed the transcriptome through high throughput sequencing. From the sequencing of mRNA from whole body tissues of Neoca
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Hsieh, Ping-Han, and 謝秉翰. "Effect of de novo transcriptome assembly on quality of read mapping and transcript quantification." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/5m3zmn.

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碩士<br>國立臺灣大學<br>生醫電子與資訊學研究所<br>105<br>Correct quantification of transcript abundance is essential to understand the functional products of the genome in different physiological conditions and developmental stages. Recently, the development of high-throughput RNA sequencing (RNA-Seq) allows the researchers to perform transcriptome analysis for the organisms without the reference genome and transcriptome. For these practical projects, de novo transcriptome assembly must be carried out prior to quantification. However, a large number of fragmented contigs and redundant sequences produced by the a
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Pei-YuanCheng and 鄭培元. "De novo Assembly and Functional Analyses of Skin Transcriptome of a Stranded Dwarf Sperm Whale (Kogia sima)." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/9h6399.

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Chang, Ching, and 張勤. "Improving completeness of de novo transcriptome assembly and gene annotation by comparison of species within the same genus." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/63057171993384642514.

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碩士<br>國立臺灣大學<br>生物產業機電工程學研究所<br>103<br>The revolutionary advances of next-generation sequencing technology not only provide high-throughput sequencing data, but also considerably facilitate studies with regard to transcriptome without a reference genome. By means of de novo assembly, assembled transcripts can be retrieved from the sequencing reads. In order to infer the protein function of the assembled sequences, one conventional approach is to utilize the sequence similarity against the protein database by BLASTx. In this study, only 49% (approximately 24,800 sequences) of the assembled Bact
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Peloakgosi-Shikwambani, Keneilwe. "Analysis of Babesia rossi transcriptome in dogs diagnosed with canine babesiosis." Diss., 2018. http://hdl.handle.net/10500/24424.

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Background: Canine babesiosis is a tick-borne disease causing detrimental health effects on the domestic dogs with huge economic impact on the owners. The most complicated form of canine babesiosis is caused by a pathogenic Babesia rossi parasite. Canine babesiosis induced by B. rossi still remains the cause of mortality and morbidity in South African dogs, yet, the transcriptomic and genomic information of this parasite species is still not available. The transcriptomic and genomic information is essential in the disease development and processes for the design of effective disease control st
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Andrews, Marc. "Signal-dependent Translation of the Platelet Transcriptome: The Roles of αIIbβ3 Integrin, Fibrinogen and Fibronectin in Platelet de novo Protein Synthesis". Thesis, 2012. http://hdl.handle.net/1807/32225.

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Although platelets are anucleate, they do inherit 1500-3000 mRNA transcripts from their megakaryocyte progenitors, in addition to all the machinery essential for protein synthesis; however, there is little understanding why platelets initiate de novo synthesis of these transcripts. Our group demonstrated that fibrinogen (Fg), a ligand of platelet Glycoprotein (GP)IIb-IIIa (αIIbβ3 integrin), is required for platelet P-selectin expression and that engagement of Fg with GPIIb-IIIa is essential for this process. The present study shows that murine platelets incubated with Fg synthesize P-selectin
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De, Castro Minique Hilda. "Sialotranscriptomics of the brown ear ticks, Rhipicephalus appendiculatus Neumann, 1901 and R. Zambeziensis Walker, Norval and Corwin, 1981, vectors of Corridor disease." Thesis, 2017. http://hdl.handle.net/10500/24735.

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Text in English<br>Corridor disease is an economically important tick-borne disease of cattle in southern Africa. The disease is caused by Theileria parva and transmitted by the vectors, Rhipicephalus appendiculatus and R. zambeziensis. There is currently no vaccine to protect cattle against T. parva that is permitted in South Africa. To develop recombinant anti-tick vaccines against Corridor disease, comprehensive databases of genes expressed in the tick’s salivary glands are required. Therefore, in Chapters 2 and 3, mRNA from the salivary glands of R. appendiculatus and R. zambeziensis was s
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