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Artykuły w czasopismach na temat „Degradation metabolites”

Autor: Grafiati

Data publikacji: 10 sierpnia 2024

Data aktualizacji: 31 lipca 2025

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1

Xia, Chaoran, Qiyuan Tian, Lingyu Kong, et al. "Metabolomics Analysis for Nitrite Degradation by the Metabolites of Limosilactobacillus fermentum RC4." Foods 11, no. 7 (2022): 1009. http://dx.doi.org/10.3390/foods11071009.

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Nitrite (NIT), a commonly used food additive, especially in pickled and cured vegetables and meat products, might cause acute and chronic diseases. Fermentation with lactic acid bacteria (LAB) is an effective method for degrading NIT and improving the flavor of pickled and cured foods. In this study, Limosilactobacillus fermentum (L. fermentum) RC4 with a high NIT degradation ability was found to degrade NIT in a new manner when compared with reported enzymatic and acid degradation, namely, metabolite degradation during fermentation in MRS broth, which shows a synergistic effect with acid to i

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Shen, C. F., J. A. Hawari, G. Ampleman, S. Thiboutot, and S. R. Guiot. "Origin ofp-cresol in the anaerobic degradation of trinitrotoluene." Canadian Journal of Microbiology 46, no. 2 (2000): 119–24. http://dx.doi.org/10.1139/w99-124.

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p-Cresol was repeatedly detected as a trace metabolite in anaerobic slurry reactors treating 2,4,6-trinitrotoluene (TNT)-contaminated soils. This study shows that p-cresol was not a metabolite of the anaerobic degradation of TNT, by using a combination of analytical techniques and13C-labelled TNT. Instead, p-cresol, an intermediate in the degradation pathway of some amino acids, was shown to be inhibited by TNT and its metabolites. The range and persistence of inhibition to p-cresol microbial degradation decreased with the level of amino-substitution of the derivatives. This explains why p-cre

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Carone, F. A., M. A. Stetler-Stevenson, V. May, A. LaBarbera, and G. Flouret. "Differences between in vitro and in vivo degradation of LHRH by rat brain and other organs." American Journal of Physiology-Endocrinology and Metabolism 253, no. 3 (1987): E317—E321. http://dx.doi.org/10.1152/ajpendo.1987.253.3.e317.

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Homogenates of brain, pituitary, liver, lung, ovary, and testes were incubated with [pyro Glu1-3,4-3H]luteinizing hormone-releasing hormone ([3H]LHRH), and the profiles of metabolites generated as a function of time were determined. After 5 min of incubation, 5 was the predominant metabolite in most homogenates. Although the profiles of metabolites varied at different time intervals, metabolites 2, 3, 4, and 5, and in some instances 7 and 9, appeared to form simultaneously and were detectable at 10 min. Neither metabolite 6 nor other larger metabolites formed initially as dominant degradation

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4

Kar, Soumya, Marinus te Pas, Leo Kruijt, Jacques Vervoort, Alfons Jansman, and Dirkjan Schokker. "Sanitary Conditions on the Farm Alters Fecal Metabolite Profile in Growing Pigs." Metabolites 12, no. 6 (2022): 538. http://dx.doi.org/10.3390/metabo12060538.

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The aim of this study was to use fecal metabolite profiling to evaluate the effects of contrasting sanitary conditions and the associated subclinical health status of pigs. We analyzed fecal metabolite profiles by nuclear magnetic resonance (1H NMR) from pigs aged 14 and 22 weeks. Pigs kept under low and high sanitary conditions differed in fecal metabolites related to the degradation of dietary starch, metabolism of the gut microbiome, and degradation of components of animal (host) origin. The metabolites that differed significantly (FDR < 0.1) were from metabolic processes involved in eit

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Wang, Qinghong, Siyu Li, Xin Wang, Zhuoyu Li, Yali Zhan, and Chunmao Chen. "Efficient Degradation of 4-Acetamidoantipyrin Using a Thermally Activated Persulfate System." Sustainability 14, no. 21 (2022): 14300. http://dx.doi.org/10.3390/su142114300.

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The extensive use of pharmaceuticals and personal care products (PPCPs) causes high concentrations of pharmaceutical metabolites to exist in aquatic environments. Though the removal of parent PPCPs has raised concerns, the degradation of pharmaceutical metabolites was rarely investigated. In this study, the degradation of 4-acetylaminoantipyrine (4-AAA), a typical dipyrone metabolite frequently detected worldwide in surface water and wastewater, was initially studied using persulfate (PDS)-based advanced oxidation processes (AOPs). Compared with commonly used activation methods of alkali, ultr

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6

Hong, Junting, Nadia Boussetta, Gérald Enderlin, Nabil Grimi, and Franck Merlier. "Real-Time Monitoring of the Atrazine Degradation by Liquid Chromatography and High-Resolution Mass Spectrometry: Effect of Fenton Process and Ultrasound Treatment." Molecules 27, no. 24 (2022): 9021. http://dx.doi.org/10.3390/molecules27249021.

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High resolution mass spectrometry (HRMS) was coupled with ultra-high-performance liquid chromatography (uHPLC) to monitor atrazine (ATZ) degradation process of Fenton/ultrasound (US) treatment in real time. Samples were automatically taken through a peristaltic pump, and then analysed by HPLC-HRMS. The injection in the mass spectrometer was performed every 4 min for 2 h. ATZ and its degradation metabolites were sampled and identified. Online Fenton experiments in different equivalents of Fenton reagents, online US experiments with/without Fe2+ and offline Fenton experiments were conducted. Hig

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Chen, Xiao-Jun, Zhi-Yuan Meng, Li Ren, et al. "Determination and Safety Assessment of Residual Spirotetramat and Its Metabolites in Amaranth (Amaranthus tricolor) and Soil by Liquid Chromatography Triple-Quadrupole Tandem Mass Spectrometry." Journal of AOAC INTERNATIONAL 101, no. 3 (2018): 848–57. http://dx.doi.org/10.5740/jaoacint.17-0216.

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Abstract With the purpose of guaranteeing the safe use of spirotetramat and preventing its potential health threats to consumers, a QuEChERS extraction method coupled with LC triple-quadrupole tandem MS was applied in this study to determine residual spirotetramat metabolites in different tissues of amaranth (Amaranthus tricolor) and in soil. The results indicate that the spirotetramat degraded into different types of metabolites that were located in different tissues of amaranth and in soil. B-keto, B-glu, and B-enol were the three most representative degradation products in the leaf of amara

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Wetzstein, Heinz-Georg, Marc Stadler, Hans-Volker Tichy, Axel Dalhoff, and Wolfgang Karl. "Degradation of Ciprofloxacin by Basidiomycetes and Identification of Metabolites Generated by the Brown Rot FungusGloeophyllum striatum." Applied and Environmental Microbiology 65, no. 4 (1999): 1556–63. http://dx.doi.org/10.1128/aem.65.4.1556-1563.1999.

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ABSTRACT Ciprofloxacin (CIP), a fluoroquinolone antibacterial drug, is widely used in the treatment of serious infections in humans. Its degradation by basidiomycetous fungi was studied by monitoring14CO2 production from [14C]CIP in liquid cultures. Sixteen species inhabiting wood, soil, humus, or animal dung produced up to 35% 14CO2 during 8 weeks of incubation. Despite some low rates of14CO2 formation, all species tested had reduced the antibacterial activity of CIP in supernatants to between 0 and 33% after 13 weeks. Gloeophyllum striatum was used to identify the metabolites formed from CIP

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9

Hosseini, Parastou Khalessi, and Sonia Michail. "COMPARING THE GUT METABOLOMIC PROFILES IN HISPANIC AND NON-HISPANIC PEDIATRIC ULCERATIVE COLITIS PATIENTS." Inflammatory Bowel Diseases 28, Supplement_1 (2022): S67. http://dx.doi.org/10.1093/ibd/izac015.109.

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Abstract BACKGROUND Diagnosis of ulcerative colitis (UC), a subgroup of inflammatory bowel disease (IBD), is frequently overlooked among the Hispanic population. Although previously thought to impact predominantly white populations, epidemiological studies have shown an increased incidence of UC among Hispanics. Other studies have also noted variations in disease phenotype when comparing Hispanic UC patients to non-Hispanic patients. These variations could be linked to differences in the gut microbiome and shifts in the metabolomic profile. This study aims to compare the profiles of Hispanic U

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Urbaniak, Magdalena, Elżbieta Mierzejewska, and Maciej Tankiewicz. "The stimulating role of syringic acid, a plant secondary metabolite, in the microbial degradation of structurally-related herbicide, MCPA." PeerJ 7 (April 10, 2019): e6745. http://dx.doi.org/10.7717/peerj.6745.

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The ability of microorganisms to degrade xenobiotics can be exploited to develop cost-effective and eco-friendly bioremediation technologies. Microorganisms can degrade almost all organic pollutants, but this process might be very slow in some cases. A promising way to enhance removal of recalcitrant xenobiotics from the environment lies in the interactions between plant exudates such as plant secondary metabolites (PSMs) and microorganisms. Although there is a considerable body of evidence that PSMs can alter the microbial community composition and stimulate the microbial degradation of xenob

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Park, Min-Kyung, Soyeon Lee, and Young-Suk Kim. "Effects of pH and Osmotic Changes on the Metabolic Expressions of Bacillus subtilis Strain 168 in Metabolite Pathways including Leucine Metabolism." Metabolites 12, no. 2 (2022): 112. http://dx.doi.org/10.3390/metabo12020112.

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Bacillus subtilis is often exposed to diverse culture conditions with the aim of improving hygiene or food quality. This can lead to changes in the volatile metabolite profiles related to the quality of fermented foods. To comprehensively interpret the associated metabolic expressions, changes in intracellular primary and extracellular secondary volatile metabolites were investigated by exposing B. subtilis to an alkaline pH (BP, pH 8.0) and a high salt concentration (BS, 1 M). In particular, B. subtilis was cultured in a leucine-enriched medium to investigate the formation of leucine-derived

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12

Sari, Ira Puspita, and Khanom Simarani. "Comparative static and shaking culture of metabolite derived from methyl red degradation by Lysinibacillus fusiformis strain W1B6." Royal Society Open Science 6, no. 7 (2019): 190152. http://dx.doi.org/10.1098/rsos.190152.

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This paper reports on the comparative characteristics and properties of the metabolites derived from methyl red (MR) decolorization by Lysinibacillus fusiformis strain W1B6 under static and shaking conditions. A batch culture system was used to investigate the effect of aeration on azoreductase activity in the biodegradation process, transformation of colour removal and the metabolite products. Biodegradation analysis was monitored using Fourier transform infrared spectroscopy and high-performance liquid chromatography while metabolites were determined using gas chromatography–mass spectroscop

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13

Katayama-Hirayama, K., S. Tobita, and K. Hirayama. "Aromatic Degradation in Yeast Rhodotorula rubra." Water Science and Technology 26, no. 3-4 (1992): 773–81. http://dx.doi.org/10.2166/wst.1992.0458.

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Aromatic degradation by two yeast strains of Rhodotorula (R.) rubra was examined. Separation and identification of phenol and protocatechuic acid (PCA) metabolites were carried out using high performance liquid chromatography (HPLC) and gas chromatography/mass spectrometry (GC/MS). For HPLC analysis of β -ketoadipic acid, 2,4-dinitrophenylhydrazone derivative was prepared. For GC/MS analysis, metabolites in the cultured broth were extracted with ethyl acetate and trimethylsilylated by N-o-bis(trimethylsilyl)acetoamide. Based on HPLC and GC/MS analyses, phenol metabolites were identified as cat

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Nag, Abhishek, Yuko Kurushima, Ruth C. E. Bowyer, et al. "Genome-wide scan identifies novel genetic loci regulating salivary metabolite levels." Human Molecular Genetics 29, no. 5 (2020): 864–75. http://dx.doi.org/10.1093/hmg/ddz308.

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Abstract Saliva, as a biofluid, is inexpensive and non-invasive to obtain, and provides a vital tool to investigate oral health and its interaction with systemic health conditions. There is growing interest in salivary biomarkers for systemic diseases, notably cardiovascular disease. Whereas hundreds of genetic loci have been shown to be involved in the regulation of blood metabolites, leading to significant insights into the pathogenesis of complex human diseases, little is known about the impact of host genetics on salivary metabolites. Here we report the first genome-wide association study

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15

Kutanovas, Simonas, Jonita Stankeviciute, Gintaras Urbelis, Daiva Tauraite, Rasa Rutkiene, and Rolandas Meskys. "Identification and Characterization of a Tetramethylpyrazine Catabolic Pathway in Rhodococcus jostii TMP1." Applied and Environmental Microbiology 79, no. 12 (2013): 3649–57. http://dx.doi.org/10.1128/aem.00011-13.

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ABSTRACTAt present, there are no published data on catabolic pathways ofN-heterocyclic compounds, in which all carbon atoms carry a substituent. We identified the genetic locus and characterized key reactions in the aerobic degradation of tetramethylpyrazine inRhodococcus jostiistrain TMP1. By comparing protein expression profiles, we identified a tetramethylpyrazine-inducible protein of 40 kDa and determined its identity by tandem mass spectrometry (MS-MS)de novosequencing. Searches against anR. jostiiTMP1 genome database allowed the identification of the tetramethylpyrazine-inducible protein

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Ronen, Zeev, and Aharon Abeliovich. "Anaerobic-Aerobic Process for Microbial Degradation of Tetrabromobisphenol A." Applied and Environmental Microbiology 66, no. 6 (2000): 2372–77. http://dx.doi.org/10.1128/aem.66.6.2372-2377.2000.

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ABSTRACT Tetrabromobisphenol A (TBBPA) is a flame retardant that is used as an additive during manufacturing of plastic polymers and electronic circuit boards. Little is known about the fate of this compound in the environment. In the current study we investigated biodegradation of TBBPA, as well as 2,4,6-tribromophenol (TBP), in slurry of anaerobic sediment from a wet ephemeral desert stream bed contaminated with chemical industry waste. Anaerobic incubation of the sediment with TBBPA and peptone-tryptone-glucose-yeast extract medium resulted in a 80% decrease in the TBBPA concentration and a

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Stratmann, Bernd, Katrin Richter, Ruichao Wang, et al. "Metabolomic Signature of Coronary Artery Disease in Type 2 Diabetes Mellitus." International Journal of Endocrinology 2017 (2017): 1–9. http://dx.doi.org/10.1155/2017/7938216.

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Coronary artery disease (CAD) is a common complication of type 2 diabetes mellitus (T2D). This case-control study was done to identify metabolites with different concentrations between T2D patients with and without CAD and to characterise implicated metabolic mechanisms relating to CAD. Fasting serum samples of 57 T2D subjects, 26 with (cases) and 31 without CAD (controls), were targeted for metabolite profiling of 163 metabolites. To assess the association between metabolite levels and CAD, partial least squares (PLS) analysis and multivariate logistic regression analysis with adjustment for

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18

Garai, Edina, Anita Risa, Emese Varga, et al. "Qualifying the T-2 Toxin-Degrading Properties of Seven Microbes with Zebrafish Embryo Microinjection Method." Toxins 12, no. 7 (2020): 460. http://dx.doi.org/10.3390/toxins12070460.

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T-2 mycotoxin degradation and detoxification efficiency of seven bacterial strains were investigated with zebrafish microinjection method in three steps ((1) determination of mycotoxin toxicity baseline, (2) examination of bacterial metabolites toxicity, (3) identification of degradation products toxicity). Toxicity of T-2 was used as a baseline of toxic effects, bacterial metabolites of strains as control of bacterial toxicity and degradation products of toxin as control of biodegradation were injected into one-cell stage embryos in the same experiment. The results of in vivo tests were check

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Birmole, Radhika, and K. Aruna. "ELUCIDATING A PATHWAY FOR DEGRADATION OF AZO DYE REACTIVE RED 120 BY BACTERIAL CONSORTIUM." Journal of Applied Biological Sciences 16, no. 3 (2022): 396–417. https://doi.org/10.5281/zenodo.7113999.

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A detailed characterization of degraded metabolites is essential for understanding the mechanisms of complex dye degradation, as a result of metabolic activity of micro-organisms. The resulting knowledge may prove insightful for designing microbial tools for the purpose of bioremediation. In the current study, an azo dye RR120, completely decolorized by a bacterial consortium RAR, was analysed for identification of degraded metabolites. For this purpose, the dye degradation was first confirmed using qualitative techniques like UV-Vis spectrophotometer and HPTLC. On confirmation of biodegradati

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Radhika, Birmole, and Aruna K. "ELUCIDATING A PATHWAY FOR DEGRADATION OF AZO DYE REACTIVE RED 120 BY BACTERIAL CONSORTIUM." Journal of Applied Biological Sciences 16, no. 3 (2022): 396–417. https://doi.org/10.71336/jabs.1042.

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A detailed characterization of degraded metabolites is essential for understanding the mechanisms of complex dye degradation, as a result of metabolic activity of micro-organisms. The resulting knowledge may prove insightful for designing microbial tools for the purpose of bioremediation. In the current study, an azo dye RR120, completely decolorized by a bacterial consortium RAR, was analysed for identification of degraded metabolites. For this purpose, the dye degradation was first confirmed using qualitative techniques like UV-Vis spectrophotometer and HPTLC. On confirmation of biodegradati

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Manna, Suman, Neera Singh, and Shashi Bala Singh. "In-vitro evaluation of rice and wheat straw biochars’ effect on pyrazosulfuron-ethyl degradation and microbial activity in rice-planted soil." Soil Research 56, no. 6 (2018): 579. http://dx.doi.org/10.1071/sr18014.

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A pot-culture evaluation of 0.5% wheat (WBC600) and rice straw (RBC600) biochar amendments on degradation of pyrazosulfuron-ethyl (PYRAZO) in rice-planted sandy loam soil was undertaken. Results suggest that PYRAZO and its metabolites persisted for longer in biochar-amended soils and degradation followed biphasic dissipation. Three metabolites of PYRAZO viz. 5-(aminosulfonyl)-1-methyl-1 H-pyrazole-4-carboxylic acid, ethyl 5-(aminosulfonyl)-1-methyl-1 H-pyrazol-4-carboxylate and 2-amino-4,6- dimethoxy pyrimidine (AP) were detected in soil. There was no significant difference in the amounts of m

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Valente-Silva, Paula, Igor Cervenka, Duarte M. S. Ferreira, et al. "Effects of Tryptophan Supplementation and Exercise on the Fate of Kynurenine Metabolites in Mice and Humans." Metabolites 11, no. 8 (2021): 508. http://dx.doi.org/10.3390/metabo11080508.

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The kynurenine pathway of tryptophan (TRP) degradation (KP) generates metabolites with effects on metabolism, immunity, and mental health. Endurance exercise training can change KP metabolites by changing the levels of KP enzymes in skeletal muscle. This leads to a metabolite pattern that favors energy expenditure and an anti-inflammatory immune cell profile and reduces neurotoxic metabolites. Here, we aimed to understand if TRP supplementation in untrained vs. trained subjects affects KP metabolite levels and biological effects. Our data show that chronic TRP supplementation in mice increases

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Sugumar, R. Wilfred, and Sandhya Sadanandan. "Combined Anaerobic-Aerobic Bacterial Degradation of Dyes." E-Journal of Chemistry 7, no. 3 (2010): 739–44. http://dx.doi.org/10.1155/2010/987362.

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Wastewaters from the dye baths of a non-formal textile-dyeing unit containing C.I. Acid Orange 7 and C.I. Reactive Red 2 were subjected to degradation in a sequential anaerobic-aerobic treatment process based on mixed culture of bacteria. The technical samples of the dyestuffs and the dye bath wastes were treated in an anaerobic reactor, using an adapted mixed culture of anaerobic microorganisms. The dyestuffs were biotransformed into colourless substituted amine metabolites in the reactor. The biotransformation was assisted by co-metabolic process. The amine metabolites did not undergo furthe

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Stefaniak, Szymon, Łukasz Wojtyla, Małgorzata Pietrowska-Borek, and Sławomir Borek. "Completing Autophagy: Formation and Degradation of the Autophagic Body and Metabolite Salvage in Plants." International Journal of Molecular Sciences 21, no. 6 (2020): 2205. http://dx.doi.org/10.3390/ijms21062205.

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Autophagy is an evolutionarily conserved process that occurs in yeast, plants, and animals. Despite many years of research, some aspects of autophagy are still not fully explained. This mostly concerns the final stages of autophagy, which have not received as much interest from the scientific community as the initial stages of this process. The final stages of autophagy that we take into consideration in this review include the formation and degradation of the autophagic bodies as well as the efflux of metabolites from the vacuole to the cytoplasm. The autophagic bodies are formed through the

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Banerjee, Priyanka, Victor Adriano Okstoft Carmelo, and Haja N. Kadarmideen. "Integrative Analysis of Metabolomic and Transcriptomic Profiles Uncovers Biological Pathways of Feed Efficiency in Pigs." Metabolites 10, no. 7 (2020): 275. http://dx.doi.org/10.3390/metabo10070275.

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Feed efficiency (FE) is an economically important trait. Thus, reliable predictors would help to reduce the production cost and provide sustainability to the pig industry. We carried out metabolome-transcriptome integration analysis on 40 purebred Duroc and Landrace uncastrated male pigs to identify potential gene-metabolite interactions and explore the molecular mechanisms underlying FE. To this end, we applied untargeted metabolomics and RNA-seq approaches to the same animals. After data quality control, we used a linear model approach to integrate the data and find significant differently c

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Zeng, Li, Nian Chen, Junlin Liao, Xu Shen, Shenghua Song, and Feng Wang. "Metabolic Analysis of Potential Key Genes Associated with Systemic Lupus Erythematosus Using Liquid Chromatography-Mass Spectrometry." Computational and Mathematical Methods in Medicine 2021 (October 4, 2021): 1–17. http://dx.doi.org/10.1155/2021/5799348.

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The biological mechanism underlying the pathogenesis of systemic lupus erythematosus (SLE) remains unclear. In this study, we found 21 proteins upregulated and 38 proteins downregulated by SLE relative to normal protein metabolism in our samples using liquid chromatography-mass spectrometry. By PPI network analysis, we identified 9 key proteins of SLE, including AHSG, VWF, IGF1, ORM2, ORM1, SERPINA1, IGF2, IGFBP3, and LEP. In addition, we identified 4569 differentially expressed metabolites in SLE sera, including 1145 reduced metabolites and 3424 induced metabolites. Bioinformatics analysis sh

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Huang, Jih-Kai, Ping-Hsun Wu, Zhao-Feng Chen, et al. "Identification of Gut Microbiome Signatures Associated with Indole Pathway in Tryptophan Metabolism in Patients Undergoing Hemodialysis." Biomolecules 14, no. 6 (2024): 623. http://dx.doi.org/10.3390/biom14060623.

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Microbiota tryptophan metabolism and the biosynthesis of indole derivatives play an important role in homeostasis and pathogenesis in the human body and can be affected by the gut microbiota. However, studies on the interplay between gut microbiota and tryptophan metabolites in patients undergoing dialysis are lacking. This study aimed to identify the gut microbiota, the indole pathway in tryptophan metabolism, and significant functional differences in ESRD patients with regular hemodialysis. We performed the shotgun metagenome sequencing of stool samples from 85 hemodialysis patients. Using t

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Qu, Chunpu, Jinyuan Chen, Lina Cao, et al. "Non-Targeted Metabolomics Reveals Patterns of Metabolic Changes during Poplar Seed Germination." Forests 10, no. 8 (2019): 659. http://dx.doi.org/10.3390/f10080659.

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Research Highlights: This study was the first to use metabolomics techniques to investigate seed germination in poplar, a model woody plant. Our results lay a foundation for uncovering changes in metabolite levels during woody plant seed germination and for understanding the underlying mechanism. Background and Objectives: Poplar is a model woody plant. Because poplar can be easily propagated asexually, the molecular mechanism of poplar seed germination has not been well studied. However, long-term asexual reproduction of poplar leads to seedlings with weak resistance, high vulnerability to de

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Witting, Michael. "Suggestions for Standardized Identifiers for Fatty Acyl Compounds in Genome Scale Metabolic Models and Their Application to the WormJam Caenorhabditis elegans Model." Metabolites 10, no. 4 (2020): 130. http://dx.doi.org/10.3390/metabo10040130.

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Genome scale metabolic models (GSMs) are a representation of the current knowledge on the metabolism of a given organism or superorganism. They group metabolites, genes, enzymes and reactions together to form a mathematical model and representation that can be used to analyze metabolic networks in silico or used for analysis of omics data. Beside correct mass and charge balance, correct structural annotation of metabolites represents an important factor for analysis of these metabolic networks. However, several metabolites in different GSMs have no or only partial structural information associ

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J, Sowmia, Dhinek A, R. Ragunathan, and Jesteena Johney. "Evaluation of Biocontrol Potentiality of Bacillus subtilis on Aflatoxins Production in Grains." Journal of Biochemistry International 12, no. 1 (2025): 70–84. https://doi.org/10.56557/jobi/2025/v12i19220.

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Aims: This study aimed to isolate Aspergillus species from peanut (Arachis hypogaea) and toor dhal (Cajanus cajan), extraction and characterize of aflatoxin, assessment of its degradation by Bacillus subtilis metabolites. Study Design: The study was conducted involving sample collection, fungal isolation, toxin extraction, and bacterial degradation analysis. Place and Duration of Study: The study was carried out at Centre for Bioscience and Nanoscience Research, Coimbatore District from December 2024 to February 2025. Methodology: Peanut and Toor dhal samples were collected from the local mark

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Miao, Dongzhi, Xuebei Wu, Kui Zuo, et al. "Non-Targeted Metabolomics Analysis of Small Molecular Metabolites in Refrigerated Goose Breast Meat." Veterinary Sciences 11, no. 12 (2024): 637. https://doi.org/10.3390/vetsci11120637.

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Poultry represents a rich source of multiple nutrients. Refrigeration is commonly employed for poultry preservation, although extended storage duration can adversely affect the meat quality. Current research on this topic has focused on the analysis of biochemical indices in chilled goose meat, with limited information on changes in metabolites that influence the quality of the meat during storage. This study used non-targeted metabolomics and the random forest algorithm to investigate metabolite changes in goose meat over an extended storage period. The results showed a significant change in

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Zhang, Wen, Yilei Wen, Zhequan Wang, Chenyang Diao, and Zhiwei Liu. "The Fungi–Bacteria Interaction Mechanism of Microbial Consortium During Efficient Lignin Degradation Based on Metabolomics Analysis." Molecules 30, no. 3 (2025): 508. https://doi.org/10.3390/molecules30030508.

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Microbial consortium degradation technology can improve the degradation efficiency and adaptability through fungi–bacteria synergism, but the mechanism of the fungi–bacteria interaction is still unclear, making it difficult to optimize the degradation process. The microbial consortium J-6, with high lignin degradation efficiency and strong environmental adaptability, was obtained in our previous research. In this study, the fungi–bacteria interacting mechanism of the microbial consortium J-6 was inferred based on metabolomics technology. The results showed that the positive interaction between

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Li, Juying, Laurel Dodgen, Qingfu Ye, and Jay Gan. "Degradation Kinetics and Metabolites of Carbamazepine in Soil." Environmental Science & Technology 47, no. 8 (2013): 3678–84. http://dx.doi.org/10.1021/es304944c.

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Barceló, Damià. "Advanced MS analysis of metabolites and degradation products." TrAC Trends in Analytical Chemistry 27, no. 10 (2008): 805–6. http://dx.doi.org/10.1016/j.trac.2008.09.005.

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DebMandal, Manisha, Shyamapada Mandal, Nishith Kumar Pal, and Aniruddha Aich. "Potential metabolites of dimethoate produced by bacterial degradation." World Journal of Microbiology and Biotechnology 24, no. 1 (2007): 69–72. http://dx.doi.org/10.1007/s11274-007-9440-5.

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Yao, Xie-Feng, Fazlurrahman Khan, Rinku Pandey, et al. "Degradation of dichloroaniline isomers by a newly isolated strain, Bacillus megaterium IMT21." Microbiology 157, no. 3 (2011): 721–26. http://dx.doi.org/10.1099/mic.0.045393-0.

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An efficient 3,4-dichloroaniline (3,4-DCA)-mineralizing bacterium has been isolated from enrichment cultures originating from a soil sample with a history of repeated exposure to diuron, a major metabolite of which is 3,4-DCA. This bacterium, Bacillus megaterium IMT21, also mineralized 2,3-, 2,4-, 2,5- and 3,5-DCA as sole sources of carbon and energy. These five DCA isomers were degraded via two different routes. 2,3-, 2,4- and 2,5-DCA were degraded via previously unknown dichloroaminophenol metabolites, whereas 3,4- and 3,5-DCA were degraded via dichloroacetanilide.

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Annweiler, E., H. H. Richnow, G. Antranikian, et al. "Naphthalene Degradation and Incorporation of Naphthalene-Derived Carbon into Biomass by the ThermophileBacillus thermoleovorans." Applied and Environmental Microbiology 66, no. 2 (2000): 518–23. http://dx.doi.org/10.1128/aem.66.2.518-523.2000.

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ABSTRACT The thermophilic aerobic bacterium Bacillus thermoleovorans Hamburg 2 grows at 60°C on naphthalene as the sole source of carbon and energy. In batch cultures, an effective substrate degradation was observed. The carbon balance, including naphthalene, metabolites, biomass, and CO2, was determined by the application of [1-13C]naphthalene. The incorporation of naphthalene-derived carbon into the bulk biomass as well as into specified biomass fractions such as fatty acids and amino acids was confirmed by coupled gas chromatography-mass spectrometry (GC-MS) and isotope analyses. Metabolite

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Loh, Zhi Hung, Diane Ouwerkerk, Athol V. Klieve, Natasha L. Hungerford, and Mary T. Fletcher. "Toxin Degradation by Rumen Microorganisms: A Review." Toxins 12, no. 10 (2020): 664. http://dx.doi.org/10.3390/toxins12100664.

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Animal feeds may contain exogenous compounds that can induce toxicity when ruminants ingest them. These toxins are secondary metabolites originating from various sources including plants, bacteria, algae and fungi. Animal feed toxins are responsible for various animal poisonings which negatively impact the livestock industry. Poisoning is more frequently reported in newly exposed, naïve ruminants while ‘experienced’ ruminants are observed to better tolerate toxin-contaminated feed. Ruminants can possess detoxification ability through rumen microorganisms with the rumen microbiome able to adapt

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Loh, Zhi Hung, Natasha L. Hungerford, Diane Ouwerkerk, Athol V. Klieve, and Mary T. Fletcher. "Identification of Acid Hydrolysis Metabolites of the Pimelea Toxin Simplexin for Targeted UPLC-MS/MS Analysis." Toxins 15, no. 9 (2023): 551. http://dx.doi.org/10.3390/toxins15090551.

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Pimelea poisoning of cattle is a unique Australian toxic condition caused by the daphnane orthoester simplexin present in native Pimelea pasture plants. Rumen microorganisms have been proposed to metabolise simplexin by enzymatic reactions, likely at the orthoester and epoxide moieties of simplexin, but a metabolic pathway has not been confirmed. This study aimed to investigate this metabolic pathway through the analysis of putative simplexin metabolites. Purified simplexin was hydrolysed with aqueous hydrochloric acid and sulfuric acid to produce target metabolites for UPLC-MS/MS analysis of

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He, Hui, Zhengfei Cao, Tao Wang, Chuyu Tang, Yuling Li, and Xiuzhang Li. "Metabolomics Combined with Physiology and Transcriptomics Reveal the Response of Samsoniella hepiali to Key Metabolic Pathways and Its Degradation Mechanism during Subculture." Antioxidants 13, no. 7 (2024): 780. http://dx.doi.org/10.3390/antiox13070780.

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During the subculture of filamentous fungi, obvious signs of degradation occur which affect the growth and development of the strain, change the content of metabolites, and interfere with gene expression. However, the specific molecular mechanism of filamentous fungi degradation is still unclear. In this study, a filamentous fungus Samsoniella hepiali was used as the research object, and it was continuously subcultured. The results showed that when the strain was subcultured to the F8 generation, the strain began to show signs of degradation, which was manifested by affecting the apparent morp

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Beauchesne, I., S. Barnabé, D. G. Cooper, and J. A. Nicell. "Plasticizers and related toxic degradation products in wastewater sludges." Water Science and Technology 57, no. 3 (2008): 367–74. http://dx.doi.org/10.2166/wst.2008.001.

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Plasticizers can persist during the treatment of wastewaters in sewage treatment plants (STPs) and can be discharged in effluents and/or accumulated in sewage sludges. For example, di-2-ethylhexyl phthalate (DEHP) is a common plasticizer that is now considered a priority pollutant and is known to accumulate in sludges. This may add constraints to the exploitation of the beneficial uses of sludges that contain significant quantities of plasticizers. Recently, it was demonstrated in studies with pure cultures that the biodegradation of plasticizers including DEHP and di-ethylhexyl adipate (DEHA)

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Billingsley, K. A., S. M. Backus, and O. P. Ward. "Production of metabolites from chlorobiphenyls by resting cells ofPseudomonasstrain LB400 after growth on different carbon sources." Canadian Journal of Microbiology 45, no. 2 (1999): 178–84. http://dx.doi.org/10.1139/w98-217.

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Cells of Pseudomonas strain LB400, grown on biphenyl, glucose, or glycerol, transformed polychlorinated biphenyl (PCB) congeners into chlorobenzoic acid (CBA) metabolites. Transformation of the PCB congeners, 2,3-chlorobiphenyl (CBP), 2,2'-CBP, 2,5,4'-CBP, and 2,4,2',4'-CBP, produced the metabolites, 2,3-CBA, 2-CBA, 4-CBA, and 2,4-CBA, respectively. Rates and extents of PCB transformation and metabolite formation were highest with biphenyl-grown cells. Intermediate rates of metabolite production were observed with glycerol-grown cells, and lowest rates of production were found with glucose-gro

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Fetyan, Nashwa A. H., Ayan A. Asair, Ismail M. Ismail, Tamer A. Elsakhawy, Sherif M. Elnagdy, and Mahmoud S. M. Mohamed. "Bacterial Degradation of Ibuprofen: Insights into Metabolites, Enzymes, and Environmental Fate Biodegradation of Ibuprofen by Achromobacter Species." Microbiology Research 15, no. 4 (2024): 2298–315. http://dx.doi.org/10.3390/microbiolres15040154.

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In recent years, pharmaceuticals have emerged as pollutants due to their incomplete degradation in sewage treatment plants and their ability to cause physiological problems in humans even at low doses. Understanding the environmental fate of pharmaceutical pollutants and the mechanisms involved in their degradation is crucial for developing strategies to mitigate their impact on ecosystems and human health. In this study, the degradation of pharmaceutical compound ibuprofen was achieved by employing two bacterial strains, Achromobacter spanius strain S11 and Achromobacter piechaudii S18, previ

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Martin, Margarita, Gerardo Mengs, Jose Luis Allende, Javier Fernandez, Ramon Alonso, and Estrella Ferrer. "Characterization of Two Novel Propachlor Degradation Pathways in Two Species of Soil Bacteria." Applied and Environmental Microbiology 65, no. 2 (1999): 802–6. http://dx.doi.org/10.1128/aem.65.2.802-806.1999.

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ABSTRACT Propachlor (2-chloro-N-isopropylacetanilide) is an acetamide herbicide used in preemergence. In this study, we isolated and characterized a soil bacterium, Acinetobacter strain BEM2, that was able to utilize this herbicide as the sole and limiting carbon source. Identification of the intermediates of propachlor degradation by this strain and characterization of new metabolites in the degradation of propachlor by a previously reported strain ofPseudomonas (PEM1) support two different propachlor degradation pathways. Washed-cell suspensions of strain PEM1 with propachlor accumulated N-i

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Xue, Moyong, Xu Gu, Yuchang Qin, Junguo Li, Qingshi Meng, and Ming Jia. "Enantioselective Behavior of Flumequine Enantiomers and Metabolites’ Identification in Sediment." Journal of Analytical Methods in Chemistry 2022 (December 2, 2022): 1–12. http://dx.doi.org/10.1155/2022/2184024.

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The enantioselective adsorption, degradation, and transformation of flumequine (FLU) enantiomers in sediment were investigated to elucidate the enantioselective environmental behaviors. The results of adsorption test showed that stereoselective differences of FLU enantiomers in sediment samples and the adsorbing capacity of S-(−)-FLU and R-(+)-FLU are higher than the racemate, and the pH values of the sediment determined the adsorption capacity. Enantioselective degradation behaviors were found under nonsterilized conditions and followed pseudo-first-order kinetic. The R-(+)-FLU was preferenti

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Fraser, Karl, Hanna Lagstrom, Shikha Pundir, David Cameron-Smith, and Nicole Roy. "Infant Feeding Frequency Impacts Human Milk Composition: A Metabolomic Analysis." Current Developments in Nutrition 4, Supplement_2 (2020): 986. http://dx.doi.org/10.1093/cdn/nzaa054_058.

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Abstract Objectives The nutritional composition of human milk is affected by many factors, including stage of lactation and time of day. Metabolomic profiling of milk provides a biochemical fingerprint of hundreds of metabolites being consumed by the infant, which may help to understand potential factors affecting infant health, growth and nutritional status. We hypothesized that frequency of daily lactations would alter the profile of metabolites and lipids in the milk. Methods Human milk samples from 630 individuals [378 mothers exclusively breast feeding and 252 mothers partially breast fee

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Barnes, V. M., S. G. Ciancio, O. Shibly, et al. "Metabolomics Reveals Elevated Macromolecular Degradation in Periodontal Disease." Journal of Dental Research 90, no. 11 (2011): 1293–97. http://dx.doi.org/10.1177/0022034511416240.

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Periodontitis is a chronic inflammatory disease characterized by tissue destruction. In the diseased oral environment, saliva has primarily been considered to act as a protectant by lubricating the tissue, mineralizing the bones, neutralizing the pH, and combating microbes. To understand the metabolic role that saliva plays in the diseased state, we performed untargeted metabolomic profiling of saliva from healthy and periodontitic individuals. Several classes of biochemicals, including dipeptide, amino acid, carbohydrate, lipids, and nucleotide metabolites, were altered, consistent with incre

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van Herwijnen, René, Dirk Springael, Pieter Slot, Harrie A. J. Govers, and John R. Parsons. "Degradation of Anthracene by Mycobacterium sp. Strain LB501T Proceeds via a Novel Pathway, through o-Phthalic Acid." Applied and Environmental Microbiology 69, no. 1 (2003): 186–90. http://dx.doi.org/10.1128/aem.69.1.186-190.2003.

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ABSTRACT Mycobacterium sp. strain LB501T utilizes anthracene as a sole carbon and energy source. We analyzed cultures of the wild-type strain and of UV-generated mutants impaired in anthracene utilization for metabolites to determine the anthracene degradation pathway. Identification of metabolites by comparison with authentic standards and transient accumulation of o-phthalic acid by the wild-type strain during growth on anthracene suggest a pathway through o-phthalic acid and protocatechuic acid. As the only productive degradation pathway known so far for anthracene proceeds through 2,3-dihy

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Gao, Xiang, Bin Liu, and Boyang Ji. "Profiling of Small Molecular Metabolites in Nostoc flagelliforme during Periodic Desiccation." Marine Drugs 17, no. 5 (2019): 298. http://dx.doi.org/10.3390/md17050298.

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The mass spectrometry-based metabolomics approach has become a powerful tool for the quantitative analysis of small-molecule metabolites in biological samples. Nostoc flagelliforme, an edible cyanobacterium with herbal value, serves as an unexploited bioresource for small molecules. In natural environments, N. flagelliforme undergoes repeated cycles of rehydration and dehydration, which are interrupted by either long- or short-term dormancy. In this study, we performed an untargeted metabolite profiling of N. flagelliforme samples at three physiological states: Dormant (S1), physiologically fu

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NEUHAUS, H. Ekkehard, and Norbert SCHULTE. "Starch degradation in chloroplasts isolated from C3 or CAM (crassulacean acid metabolism)-induced Mesembryanthemum crystallinum L." Biochemical Journal 318, no. 3 (1996): 945–53. http://dx.doi.org/10.1042/bj3180945.

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C3 or crassulacean acid metabolism (CAM)-induced Mesembryanthemum crystallinum plants perform nocturnal starch degradation which is linear with time. To analyse the composition of metabolites released by isolated leaf chloroplasts during starch degradation we developed a protocol for the purification of starch-containing plastids. Isolated chloroplasts from C3 or CAM-induced M. crystallinum plants are also able to degrade starch. With respect to the endogenous starch content of isolated plastids the rate of starch degradation in these organelles is close to the observed rates of starch degrada

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